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Novel mechanism of bacteriocin secretion and immunity carried out by lactococcal multidrug resistance proteins
(Amer Soc Biochemistry Molecular Biology Inc, Bethesda, 2003)
A natural isolate of Lactococcus lactis was shown to produce two narrow spectrum class II bacteriocins, designated LsbA and LsbB. The cognate genes are located on a 5.6-kb plasmid within a gene cluster specifying LmrB, an ...
Use of induced sputum for the diagnosis of influenza and infections in asthma: a comparison of diagnostic techniques
(Elsevier, Amsterdam, 2003)
Background: Influenza (Flu) and respiratory syncytial virus (RSV) are important viral pathogens that cause lower respiratory tract infections and severe exacerbations of asthma. Molecular biological techniques are permitting ...
Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds
(Amer Chemical Soc, Washington, 2003)
The pepstatin A sensitive acidic proteolytic activity of total protein extracts of buckwheat seeds has been analyzed in developing, mature, and germinating seeds by activity measurements as well as by electrophoretic and ...
Expression and characterization of human interferon-beta I in the methylotrophic yeast Pichia pastoris
(Wiley, Hoboken, 2003)
We describe the heterologous expression of a human interferon-beta1 in the methylotrophic yeast Pichia pastoris. Biologically active recombinant human interferon-beta1 (rHulFN-beta1) was secreted from shake-flask-grown P ...
High yield preparation of genomic DNA from Streptomyces
(Eaton Publishing Co, Natick, 2003)
Modulation of SOX2 and SOX3 gene expression during differentiation of human neuronal precursor cell line NTERA2
(Springer, Dordrecht, 2003)
The SOX genes comprise a family of transcriptional regulators implicated in the control of nervous system development. The developing brain is the major site of expression of many Sox genes. Sox2 and Sox3 genes are ...
Identification and genetic characterization of a novel proteinase, PrtR, from the human isolate Lactobacillus rhamnosus BGT10
(Amer Soc Microbiology, Washington, 2003)
A novel proteinase, PrtR, produced by the human vaginal isolate Lactobacillus rhamnosus strain BGT10 was identified and genetically characterized. The prtR gene and flanking regions were cloned and sequenced. The deduced ...
The BRCA2-interacting protein DSS1 is vital for DNA repair, recombination, and genome stability in Ustilago maydis
(Cell Press, Cambridge, 2003)
DSS1 encodes a small acidic protein shown in recent structural studies to interact with the DNA binding domain of BRCA2. Here we report that an ortholog of DSS1 is present in Ustilago maydis and associates with Brh2, the ...
High frequency transformation of the amphotericin-producing bacterium Streptomyces nodosus
(Elsevier, Amsterdam, 2003)
This study has investigated DNA transformation in the Amphotericin-producing organism Streptomyces nodosus. Amphotericin B is an antifungal drug with severe side effects in humans and the availability of structural variants ...
The LysR-type transcriptional regulator CysB controls the repression of hslJ transcription in Escherichia coli
(Microbiology Soc, London, 2003)
The LysR-type transcriptional regulator (LTTR) CysB is a transcription factor in Escherichia coli cells, where as a homotetramer it binds the target promoter regions and activates the genes involved in sulphur utilization ...