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Characterization of an aspartic proteinase activity in buckwheat (Fagopyrum esculentum moench) seeds
(Amer Chemical Soc, Washington, 2003)
The pepstatin A sensitive acidic proteolytic activity of total protein extracts of buckwheat seeds has been analyzed in developing, mature, and germinating seeds by activity measurements as well as by electrophoretic and ...
Izolovanje i analiza strukture gena koji kodira novi tip aspartične proteinaze semena heljde (Fagopyrum esculentum Moench) / Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)
(Srpsko biološko društvo, Beograd, i dr., 2007)
Iz biblioteke cDNK semena heljde u srednjoj fazi razvića izolovan je gen koji kodira novi tip aspartične proteinaze. Analizom sekvence ove cDNK (Fe-APL1) uočeno je odsustvo domena karakterističnog samo za biljne aspartične ...
Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions
(Elsevier Gmbh, Munich, 2004)
The buckwheat metallothionein-like (MT3) gene expression was studied throughout seed and leaf development, as well as under the influence of different external stimuli. MT3 mRNAs were detected from the early stage of seed ...
Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds
(Amer Chemical Soc, Washington, 2004)
An 8S storage globulin from buckwheat seed, which resembles the structure and features common to the vicilin-like family of seed storage proteins, was analyzed for this paper. It was found that expression of the 8S globulin ...
Analiza strukture dva transkripta gena za metalotionein heljde i ispitivanje njihove ekspresije tokom hipoksije korišćenjem tehnologije Real Time RT-PCR / Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology
(Srpsko biološko društvo, Beograd, i dr., 2008)
Metalotioneini (MT) pripadaju velikoj grupi proteina male molekulske težine bogatih cisteinom, izražene sposobnosti za vezivanje jona metala, uključenih u procese održavanja homeostaze metalnih jona i detoksifikacije od ...
The metallothionein-like gene from buckwheat: Structural and functional analysis of the promoter region
(Univ Chicago Press, Chicago, 2005)
The buckwheat ( Fagopyrum esculentum Moench) metallothionein- like gene was cloned and its 59 regulatory region was examined. Computer analysis of this region using overlapping data from three different databases predicted ...
Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis
(Elsevier Gmbh, Urban & Fischer Verlag, Jena, 2008)
Two types of aspartic proteinase (AP) genes have been isolated from the cDNA library of developing buckwheat seeds. Analysis of their sequences showed that one of these, FeAP9, resembled the structure and shared high ...
Characterization and evolutionary relationship of methionine-rich legumin-like protein from buckwheat
(Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux, 2004)
We have isolated and characterized a full-length cDNA for legumin-like storage polypeptide from buckwheat seed (Fagopyrum esculentum Moench) and compared its deduced amino acid sequence with those from different representatives ...
Isolation and computer analysis of the 5 '-regulatory region of the seed storage protein gene from buckwheat (Fagopyrum esculentum Moench)
(Amer Chemical Soc, Washington, 2005)
Using the modified rapid amplification of cDNA ends (5'-RACE) approach, a fragment containing the 955 bp long 5'-regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of ...
Aspartične proteinaze semena heljde (Fagopyrum esculentum moench) - prečišćavanje i svojstva enzima 47 kDa / Proteinases from buckwheat (Fagopyrum esculentum moench) seeds: Purification and properties of the 47 kDa enzyme
(Srpsko biološko društvo, Beograd, i dr., 2006)
Analizirane su aspartične proteinaze semena heljde. Upotrebom pepstatin A afinitetne hromatografije, iz zrelog semena izdvojene su tri forme aspartičnih proteinaza, od 47 kDa, 40 kDa i 28 kDa, dok je u ekstraktu nezrelog ...