TY  - JOUR
AU  - Rakocević, Jelena
AU  - Kojić, Snežana
AU  - Orlić, Dejan
AU  - Stanković, Goran
AU  - Ostojić, Miodrag
AU  - Petrović, Olga
AU  - Zaletel, Ivan
AU  - Puskas, Nela
AU  - Todorović, Vera
AU  - Labudović-Borović, Milica
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/979
AB  - Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental and Molecular Pathology
T1  - Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction
EP  - 38
IS  - 1
SP  - 31
VL  - 100
DO  - 10.1016/j.yexmp.2015.11.028
ER  - 

@article{
author = "Rakocević, Jelena and Kojić, Snežana and Orlić, Dejan and Stanković, Goran and Ostojić, Miodrag and Petrović, Olga and Zaletel, Ivan and Puskas, Nela and Todorović, Vera and Labudović-Borović, Milica",
year = "2016",
abstract = "Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental and Molecular Pathology",
title = "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction",
pages = "38-31",
number = "1",
volume = "100",
doi = "10.1016/j.yexmp.2015.11.028"
}

Rakocević, J., Kojić, S., Orlić, D., Stanković, G., Ostojić, M., Petrović, O., Zaletel, I., Puskas, N., Todorović, V.,& Labudović-Borović, M.. (2016). Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 100(1), 31-38.
https://doi.org/10.1016/j.yexmp.2015.11.028

Rakocević J, Kojić S, Orlić D, Stanković G, Ostojić M, Petrović O, Zaletel I, Puskas N, Todorović V, Labudović-Borović M. Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology. 2016;100(1):31-38.
doi:10.1016/j.yexmp.2015.11.028 .

Rakocević, Jelena, Kojić, Snežana, Orlić, Dejan, Stanković, Goran, Ostojić, Miodrag, Petrović, Olga, Zaletel, Ivan, Puskas, Nela, Todorović, Vera, Labudović-Borović, Milica, "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction" in Experimental and Molecular Pathology, 100, no. 1 (2016):31-38,
https://doi.org/10.1016/j.yexmp.2015.11.028 . .

TY  - JOUR
AU  - Tomić, Sergej
AU  - Đokić, Jelena
AU  - Vasilijić, Sasa
AU  - Vucević, Dragana
AU  - Todorović, Vera
AU  - Supić, Gordana
AU  - Čolić, Miodrag
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/529
AB  - Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources of MSCs have been sought. In this work, we studied MSCs from dental pulp (DP-MSCs) and dental follicle (DF-MSCs), isolated from the same tooth/donor, to define differences in their phenotypic properties, differentiation potential, and immunomodulatory activities. Both cell types showed colony-forming ability and expressed typical MSCs markers, but differed in the levels of their expression. DF-MSCs proliferated faster, contained cells larger in diameter, exhibited a higher potential to form adipocytes and a lower potential to form chondrocytes and osteoblasts, compared with DP-MSCs. In contrast to DF-MSCs, DP-MSCs produced the transforming growth factor (TGF)-beta and suppressed proliferation of peripheral blood mononuclear cells, which could be neutralized with anti-TGF-beta antibody. The treatment with toll-like receptor 3 (TLR3) agonist augmented the suppressive potential of both cell types and potentiated TGF-beta and interleukin-6 secretions by these cells. TLR4 agonist augmented the suppressive potential of DF-MSCs and increased TGF-beta production, but abrogated the immunosuppressive activity of DP-MSCs by inhibiting TGF-beta production and the expression of indolamine-2,3-dioxygenase-1. Some of these effects correlated with the higher expression of TLR3 and TLR4 by DP-MSCs compared with DF-MSCs. When transplanted in imunocompetent xenogenic host, both cell types induced formation of granulomatous tissue. In conclusion, our results suggest that dental MSCs are functionally different and each of these functions should be further explored in vivo before their specific biomedical applications.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Stem Cells and Development
T1  - Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists
EP  - 708
IS  - 4
SP  - 695
VL  - 20
DO  - 10.1089/scd.2010.0145
ER  - 

@article{
author = "Tomić, Sergej and Đokić, Jelena and Vasilijić, Sasa and Vucević, Dragana and Todorović, Vera and Supić, Gordana and Čolić, Miodrag",
year = "2011",
abstract = "Adult mesenchymal stem cells (MSCs) have recently become a potent tool in regenerative medicine. Due to certain shortcomings of obtaining bone marrow MSCs, alternate sources of MSCs have been sought. In this work, we studied MSCs from dental pulp (DP-MSCs) and dental follicle (DF-MSCs), isolated from the same tooth/donor, to define differences in their phenotypic properties, differentiation potential, and immunomodulatory activities. Both cell types showed colony-forming ability and expressed typical MSCs markers, but differed in the levels of their expression. DF-MSCs proliferated faster, contained cells larger in diameter, exhibited a higher potential to form adipocytes and a lower potential to form chondrocytes and osteoblasts, compared with DP-MSCs. In contrast to DF-MSCs, DP-MSCs produced the transforming growth factor (TGF)-beta and suppressed proliferation of peripheral blood mononuclear cells, which could be neutralized with anti-TGF-beta antibody. The treatment with toll-like receptor 3 (TLR3) agonist augmented the suppressive potential of both cell types and potentiated TGF-beta and interleukin-6 secretions by these cells. TLR4 agonist augmented the suppressive potential of DF-MSCs and increased TGF-beta production, but abrogated the immunosuppressive activity of DP-MSCs by inhibiting TGF-beta production and the expression of indolamine-2,3-dioxygenase-1. Some of these effects correlated with the higher expression of TLR3 and TLR4 by DP-MSCs compared with DF-MSCs. When transplanted in imunocompetent xenogenic host, both cell types induced formation of granulomatous tissue. In conclusion, our results suggest that dental MSCs are functionally different and each of these functions should be further explored in vivo before their specific biomedical applications.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Stem Cells and Development",
title = "Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists",
pages = "708-695",
number = "4",
volume = "20",
doi = "10.1089/scd.2010.0145"
}

Tomić, S., Đokić, J., Vasilijić, S., Vucević, D., Todorović, V., Supić, G.,& Čolić, M.. (2011). Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists. in Stem Cells and Development
Mary Ann Liebert, Inc, New Rochelle., 20(4), 695-708.
https://doi.org/10.1089/scd.2010.0145

Tomić S, Đokić J, Vasilijić S, Vucević D, Todorović V, Supić G, Čolić M. Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists. in Stem Cells and Development. 2011;20(4):695-708.
doi:10.1089/scd.2010.0145 .

Tomić, Sergej, Đokić, Jelena, Vasilijić, Sasa, Vucević, Dragana, Todorović, Vera, Supić, Gordana, Čolić, Miodrag, "Immunomodulatory Properties of Mesenchymal Stem Cells Derived from Dental Pulp and Dental Follicle are Susceptible to Activation by Toll-Like Receptor Agonists" in Stem Cells and Development, 20, no. 4 (2011):695-708,
https://doi.org/10.1089/scd.2010.0145 . .