TY  - JOUR
AU  - Vojnović, Sandra
AU  - Aleksić, Ivana
AU  - Ilić-Tomić, Tatjana
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://doi.org/10.1007/s00253-023-12900-x
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2314
AB  - The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.
PB  - Springer Nature
T2  - Applied Microbiology and Biotechnology
T1  - Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes
IS  - 1
SP  - 185
VL  - 108
DO  - 10.1007/s00253-023-12900-x
ER  - 

@article{
author = "Vojnović, Sandra and Aleksić, Ivana and Ilić-Tomić, Tatjana and Stevanović, Milena and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.",
publisher = "Springer Nature",
journal = "Applied Microbiology and Biotechnology",
title = "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes",
number = "1",
pages = "185",
volume = "108",
doi = "10.1007/s00253-023-12900-x"
}

Vojnović, S., Aleksić, I., Ilić-Tomić, T., Stevanović, M.,& Nikodinović-Runić, J.. (2024). Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology
Springer Nature., 108(1), 185.
https://doi.org/10.1007/s00253-023-12900-x

Vojnović S, Aleksić I, Ilić-Tomić T, Stevanović M, Nikodinović-Runić J. Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology. 2024;108(1):185.
doi:10.1007/s00253-023-12900-x .

Vojnović, Sandra, Aleksić, Ivana, Ilić-Tomić, Tatjana, Stevanović, Milena, Nikodinović-Runić, Jasmina, "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes" in Applied Microbiology and Biotechnology, 108, no. 1 (2024):185,
https://doi.org/10.1007/s00253-023-12900-x . .

TY  - JOUR
AU  - Andrejević, Tina
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Počkaj, Marta
AU  - Zlatar, Matija
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1789
AB  - Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T2  - RSC Advances
T1  - Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex
EP  - 4393
IS  - 7
SP  - 4376
VL  - 13
DO  - 10.1039/D2RA07401J
ER  - 

@article{
author = "Andrejević, Tina and Aleksić, Ivana and Kljun, Jakob and Počkaj, Marta and Zlatar, Matija and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances, RSC Advances",
title = "Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex",
pages = "4393-4376",
number = "7",
volume = "13",
doi = "10.1039/D2RA07401J"
}

Andrejević, T., Aleksić, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnović, S., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex. in RSC Advances
Royal Society of Chemistry., 13(7), 4376-4393.
https://doi.org/10.1039/D2RA07401J

Andrejević T, Aleksić I, Kljun J, Počkaj M, Zlatar M, Vojnović S, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex. in RSC Advances. 2023;13(7):4376-4393.
doi:10.1039/D2RA07401J .

Andrejević, Tina, Aleksić, Ivana, Kljun, Jakob, Počkaj, Marta, Zlatar, Matija, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Miloš, Glišić, Biljana, "Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex" in RSC Advances, 13, no. 7 (2023):4376-4393,
https://doi.org/10.1039/D2RA07401J . .

TY  - DATA
AU  - Andrejević, Tina
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Počkaj, Marta
AU  - Zlatar, Matija
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1791
AB  - Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T2  - RSC Advances
T1  - Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J
EP  - 4393
IS  - 7
SP  - 4376
VL  - 13
DO  - 10.1039/D2RA07401J
ER  - 

@misc{
author = "Andrejević, Tina and Aleksić, Ivana and Kljun, Jakob and Počkaj, Marta and Zlatar, Matija and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances, RSC Advances",
title = "Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J",
pages = "4393-4376",
number = "7",
volume = "13",
doi = "10.1039/D2RA07401J"
}

Andrejević, T., Aleksić, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnović, S., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2023). Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J. in RSC Advances
Royal Society of Chemistry., 13(7), 4376-4393.
https://doi.org/10.1039/D2RA07401J

Andrejević T, Aleksić I, Kljun J, Počkaj M, Zlatar M, Vojnović S, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J. in RSC Advances. 2023;13(7):4376-4393.
doi:10.1039/D2RA07401J .

Andrejević, Tina, Aleksić, Ivana, Kljun, Jakob, Počkaj, Marta, Zlatar, Matija, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Miloš, Glišić, Biljana, "Supplementary data for article:Andrejević, T. P., Aleksic, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnovic, S., Nikodinovic-Runic, J., Turel, I., Djuran, M. I., & Glišić, B. Đ. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): The influence of the metal ion on the antimicrobial potential of the complex. RSC Advances, 13(7), 4376–4393. https://doi.org/10.1039/D2RA07401J" in RSC Advances, 13, no. 7 (2023):4376-4393,
https://doi.org/10.1039/D2RA07401J . .

TY  - JOUR
AU  - Başpınar, Yasemin
AU  - Gürbüz, Perihan
AU  - Dilem Doğan, Şengül
AU  - Gözde Gündüz, Miyase
AU  - Aleksić, Ivana
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Yavuz Paksoy, Mehmet
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/cbdv.202300134
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1798
AB  - This is the first report on the separation and biological assessment of all metabolites derived from Pulicaria armena (Asteraceae) which is an endemic species narrowly distributed in the eastern part of Turkey. The phytochemical analysis of P. armena resulted in the identification of one simple phenolic glucoside together with eight flavon and flavonol derivatives whose chemical structures were elucidated by NMR experiments and by the comparison of the spectral data with the relevant literature. The screening of all molecules for their antimicrobial, anti-quorum sensing, and cytotoxic activities revealed the biological potential of some of the isolated compounds. Additionally, quorum sensing inhibitory activity of quercetagetin 5,7,3’ trimethyl ether was supported by molecular docking studies in the active site of LasR which is the primary regulator of this cell-to-cell communication system in bacteria. Lastly, the critical molecular properties indicating drug-likeness of the compounds isolated from P. armena were predicted. As microbial infections can be a serious problem for cancer patients with compromised immune systems, this comprehensive phytochemical research on P. armena with its anti-quorum sensing and cytotoxic compounds can provide a new approach to the treatment.
T2  - Chemistry & Biodiversity
T1  - Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)
SP  - e202300134
VL  - 20
DO  - 10.1002/cbdv.202300134
ER  - 

@article{
author = "Başpınar, Yasemin and Gürbüz, Perihan and Dilem Doğan, Şengül and Gözde Gündüz, Miyase and Aleksić, Ivana and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Yavuz Paksoy, Mehmet",
year = "2023",
abstract = "This is the first report on the separation and biological assessment of all metabolites derived from Pulicaria armena (Asteraceae) which is an endemic species narrowly distributed in the eastern part of Turkey. The phytochemical analysis of P. armena resulted in the identification of one simple phenolic glucoside together with eight flavon and flavonol derivatives whose chemical structures were elucidated by NMR experiments and by the comparison of the spectral data with the relevant literature. The screening of all molecules for their antimicrobial, anti-quorum sensing, and cytotoxic activities revealed the biological potential of some of the isolated compounds. Additionally, quorum sensing inhibitory activity of quercetagetin 5,7,3’ trimethyl ether was supported by molecular docking studies in the active site of LasR which is the primary regulator of this cell-to-cell communication system in bacteria. Lastly, the critical molecular properties indicating drug-likeness of the compounds isolated from P. armena were predicted. As microbial infections can be a serious problem for cancer patients with compromised immune systems, this comprehensive phytochemical research on P. armena with its anti-quorum sensing and cytotoxic compounds can provide a new approach to the treatment.",
journal = "Chemistry & Biodiversity",
title = "Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)",
pages = "e202300134",
volume = "20",
doi = "10.1002/cbdv.202300134"
}

Başpınar, Y., Gürbüz, P., Dilem Doğan, Ş., Gözde Gündüz, M., Aleksić, I., Vojnović, S., Nikodinović-Runić, J.,& Yavuz Paksoy, M.. (2023). Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae). in Chemistry & Biodiversity, 20, e202300134.
https://doi.org/10.1002/cbdv.202300134

Başpınar Y, Gürbüz P, Dilem Doğan Ş, Gözde Gündüz M, Aleksić I, Vojnović S, Nikodinović-Runić J, Yavuz Paksoy M. Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae). in Chemistry & Biodiversity. 2023;20:e202300134.
doi:10.1002/cbdv.202300134 .

Başpınar, Yasemin, Gürbüz, Perihan, Dilem Doğan, Şengül, Gözde Gündüz, Miyase, Aleksić, Ivana, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Yavuz Paksoy, Mehmet, "Investigation of Antimicrobial, Anti-Quorum Sensing, and Cytotoxic Activities of Flavonoids Isolated from Pulicaria armena Boiss. & Kotschy ex Boiss. (Asteraceae)" in Chemistry & Biodiversity, 20 (2023):e202300134,
https://doi.org/10.1002/cbdv.202300134 . .

TY  - JOUR
AU  - Duarah, Rituparna
AU  - Aleksić, Ivana
AU  - Milivojević, Dušan
AU  - Rameshkumar, Saranya
AU  - Nikodinović-Runić, Jasmina
AU  - Padamati, Ramesh Babu
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2079
AB  - Novel bio-based, biodegradable packaging material was developed with multi-functional properties of good mechanical strength, potent biocompatibility, and antifungal attributes, predominantly against fungi vital in food spoilage. Biodegradable polymer composites were prepared with a natural antifungal agent, nystatin (Nyst), by melt processing technique for the first time. Initially, Polycaprolactone (PCL) was melt-mixed with various percentages of nystatin to produce nystatin-encapsulated PCL composites (PCL/Nyst). The as-prepared PCL/Nyst composites were melt-mixed with polylactic acid (PLA) to produce nystatin PLA/PCL blend composites. Subsequently, the prepared composites were compression molded in the form of films (1 mm) for further characterization. The composite's structural properties were evaluated by SEM, FTIR, mechanical and thermal studies. In addition, the composites were tested in vitro against a panel of pathogenic fungi and for antibiofilm attributes, specifically against three Candida species (C. albicans ATCC10231, C. parapsilosis ATCC22019, and C. glabrata ATCC2001), and foodborne Penicillium sp. All the composites containing 2 to 20 wt% nystatin displayed good activity and sustained nystatin release for up to 4 days. Thus, the overall study demonstrates the potential application of natural antifungal agents in biodegradable polymers to produce novel composite films for antimicrobial packaging without inducing any toxicity, judged from the toxicity assay using nematode Caenorhabditis elegans.
PB  - Wiley
T2  - Journal of Applied Polymer Science
T1  - Development of nystatin-based antifungal, biodegradable polymer composite materials for food packaging via. melt processing approach
SP  - e54663
DO  - 10.1002/app.54663
ER  - 

@article{
author = "Duarah, Rituparna and Aleksić, Ivana and Milivojević, Dušan and Rameshkumar, Saranya and Nikodinović-Runić, Jasmina and Padamati, Ramesh Babu",
year = "2023",
abstract = "Novel bio-based, biodegradable packaging material was developed with multi-functional properties of good mechanical strength, potent biocompatibility, and antifungal attributes, predominantly against fungi vital in food spoilage. Biodegradable polymer composites were prepared with a natural antifungal agent, nystatin (Nyst), by melt processing technique for the first time. Initially, Polycaprolactone (PCL) was melt-mixed with various percentages of nystatin to produce nystatin-encapsulated PCL composites (PCL/Nyst). The as-prepared PCL/Nyst composites were melt-mixed with polylactic acid (PLA) to produce nystatin PLA/PCL blend composites. Subsequently, the prepared composites were compression molded in the form of films (1 mm) for further characterization. The composite's structural properties were evaluated by SEM, FTIR, mechanical and thermal studies. In addition, the composites were tested in vitro against a panel of pathogenic fungi and for antibiofilm attributes, specifically against three Candida species (C. albicans ATCC10231, C. parapsilosis ATCC22019, and C. glabrata ATCC2001), and foodborne Penicillium sp. All the composites containing 2 to 20 wt% nystatin displayed good activity and sustained nystatin release for up to 4 days. Thus, the overall study demonstrates the potential application of natural antifungal agents in biodegradable polymers to produce novel composite films for antimicrobial packaging without inducing any toxicity, judged from the toxicity assay using nematode Caenorhabditis elegans.",
publisher = "Wiley",
journal = "Journal of Applied Polymer Science",
title = "Development of nystatin-based antifungal, biodegradable polymer composite materials for food packaging via. melt processing approach",
pages = "e54663",
doi = "10.1002/app.54663"
}

Duarah, R., Aleksić, I., Milivojević, D., Rameshkumar, S., Nikodinović-Runić, J.,& Padamati, R. B.. (2023). Development of nystatin-based antifungal, biodegradable polymer composite materials for food packaging via. melt processing approach. in Journal of Applied Polymer Science
Wiley., e54663.
https://doi.org/10.1002/app.54663

Duarah R, Aleksić I, Milivojević D, Rameshkumar S, Nikodinović-Runić J, Padamati RB. Development of nystatin-based antifungal, biodegradable polymer composite materials for food packaging via. melt processing approach. in Journal of Applied Polymer Science. 2023;:e54663.
doi:10.1002/app.54663 .

Duarah, Rituparna, Aleksić, Ivana, Milivojević, Dušan, Rameshkumar, Saranya, Nikodinović-Runić, Jasmina, Padamati, Ramesh Babu, "Development of nystatin-based antifungal, biodegradable polymer composite materials for food packaging via. melt processing approach" in Journal of Applied Polymer Science (2023):e54663,
https://doi.org/10.1002/app.54663 . .

TY  - JOUR
AU  - Garcia, Eduardo Lanzagorta
AU  - Mojićević, Marija
AU  - Milivojević, Dušan
AU  - Aleksic, Ivana
AU  - Vojnović, Sandra
AU  - Stevanović, Milena
AU  - Murray, James
AU  - Attallah, Olivia Adly
AU  - Devine, Declan
AU  - Fournet, Margaret Brennan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1658
AB  - Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates
IS  - 20
SP  - 12198
VL  - 23
DO  - 10.3390/ijms232012198
ER  - 

@article{
author = "Garcia, Eduardo Lanzagorta and Mojićević, Marija and Milivojević, Dušan and Aleksic, Ivana and Vojnović, Sandra and Stevanović, Milena and Murray, James and Attallah, Olivia Adly and Devine, Declan and Fournet, Margaret Brennan",
year = "2022",
abstract = "Curcumin and triangular silver nanoplates (TSNP)-incorporated bacterial cellulose (BC) films present an ideal antimicrobial material for biomedical applications as they afford a complete set of requirements, including a broad range of long-lasting potency and superior efficacy antimicrobial activity, combined with low toxicity. Here, BC was produced by Komagataeibacter medellinensis ID13488 strain in the presence of curcumin in the production medium (2 and 10%). TSNP were incorporated in the produced BC/curcumin films using ex situ method (21.34 ppm) and the antimicrobial activity was evaluated against Escherichia coli ATCC95922 and Staphylococcus aureus ATCC25923 bacterial strains. Biological activity of these natural products was assessed in cytotoxicity assay against lung fibroblasts and in vivo using Caenorhabditis elegans and Danio rerio as model organisms. Derived films have shown excellent antimicrobial performance with growth inhibition up to 67% for E. coli and 95% for S. aureus. In a highly positive synergistic interaction, BC films with 10% curcumin and incorporated TSNP have shown reduced toxicity with 80% MRC5 cells survival rate. It was shown that only 100% concentrations of film extracts induce low toxicity effect on model organisms’ development. The combined and synergistic advanced anti-infective functionalities of the curcumin and TSNP incorporated in BC have a high potential for development for application within the clinical setting.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates",
number = "20",
pages = "12198",
volume = "23",
doi = "10.3390/ijms232012198"
}

Garcia, E. L., Mojićević, M., Milivojević, D., Aleksic, I., Vojnović, S., Stevanović, M., Murray, J., Attallah, O. A., Devine, D.,& Fournet, M. B.. (2022). Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences, 23(20), 12198.
https://doi.org/10.3390/ijms232012198

Garcia EL, Mojićević M, Milivojević D, Aleksic I, Vojnović S, Stevanović M, Murray J, Attallah OA, Devine D, Fournet MB. Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates. in International Journal of Molecular Sciences. 2022;23(20):12198.
doi:10.3390/ijms232012198 .

Garcia, Eduardo Lanzagorta, Mojićević, Marija, Milivojević, Dušan, Aleksic, Ivana, Vojnović, Sandra, Stevanović, Milena, Murray, James, Attallah, Olivia Adly, Devine, Declan, Fournet, Margaret Brennan, "Enhanced Antimicrobial Activity of Biocompatible Bacterial Cellulose Films via Dual Synergistic Action of Curcumin and Triangular Silver Nanoplates" in International Journal of Molecular Sciences, 23, no. 20 (2022):12198,
https://doi.org/10.3390/ijms232012198 . .

TY  - JOUR
AU  - Ferrero, Pablo
AU  - Attallah, Olivia A.
AU  - Angel Valera, Miguel
AU  - Aleksić, Ivana
AU  - Azeem, Muhammad
AU  - Nikodinović-Runić, Jasmina
AU  - Fournet, Margaret Brennan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1757
AB  - An energy-efficient high throughput pre-treatment of low-density polyethylene (LDPE) using a fast, reactive extrusion (REX) assisted oxidation technique followed by bacterial attachment as an indicator for bio-amenability was studied. Silicon dioxide (SiO2) was selected as a model oxidizing and catalytic reagent with the REX process demonstrated to be effective both in the presence and absence of the catalyst. Optimized 5-min duration pre-treatment conditions were determined using Box-Behnken design (BBD) with respect to screws speed, operating temperature, and concentration of SiO2. The crystallinity index, carbonyl index and weight loss (%) of LDPE were used as the studied responses for BDD. FTIR and DSC spectra of the residual LDPE obtained after pre-treatment with the REX assisted oxidation technique showed a significant increase in residual LDPE carbonyl index from 0 to 1.04 and a decrease of LDPE crystallinity index from 29 to 18%. Up to fivefold molecular weight reductions were also demonstrated using gel permeation chromatography. Optimum LDPE pre-treatment with a duration of 5 min was obtained at low screw speed (50 rpm), operating temperature of 380-390 degrees C and variable concentration of SiO2 (0 and 2% (w/w)) indicating that effective pre-treatment can occur under noncatalytic and catalysed conditions. Biofilms were successfully formed on pre-treated LDPE samples after 14 days of incubation. Furthermore, the technique proposed in this study is expected to provide a high throughput approach for pre-treatment of pervasive recalcitrant PE-based plastics to reduce their bio inertness.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation
EP  - 2846
IS  - 7
SP  - 2837
VL  - 30
DO  - 10.1007/s10924-022-02400-w
ER  - 

@article{
author = "Ferrero, Pablo and Attallah, Olivia A. and Angel Valera, Miguel and Aleksić, Ivana and Azeem, Muhammad and Nikodinović-Runić, Jasmina and Fournet, Margaret Brennan",
year = "2022",
abstract = "An energy-efficient high throughput pre-treatment of low-density polyethylene (LDPE) using a fast, reactive extrusion (REX) assisted oxidation technique followed by bacterial attachment as an indicator for bio-amenability was studied. Silicon dioxide (SiO2) was selected as a model oxidizing and catalytic reagent with the REX process demonstrated to be effective both in the presence and absence of the catalyst. Optimized 5-min duration pre-treatment conditions were determined using Box-Behnken design (BBD) with respect to screws speed, operating temperature, and concentration of SiO2. The crystallinity index, carbonyl index and weight loss (%) of LDPE were used as the studied responses for BDD. FTIR and DSC spectra of the residual LDPE obtained after pre-treatment with the REX assisted oxidation technique showed a significant increase in residual LDPE carbonyl index from 0 to 1.04 and a decrease of LDPE crystallinity index from 29 to 18%. Up to fivefold molecular weight reductions were also demonstrated using gel permeation chromatography. Optimum LDPE pre-treatment with a duration of 5 min was obtained at low screw speed (50 rpm), operating temperature of 380-390 degrees C and variable concentration of SiO2 (0 and 2% (w/w)) indicating that effective pre-treatment can occur under noncatalytic and catalysed conditions. Biofilms were successfully formed on pre-treated LDPE samples after 14 days of incubation. Furthermore, the technique proposed in this study is expected to provide a high throughput approach for pre-treatment of pervasive recalcitrant PE-based plastics to reduce their bio inertness.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation",
pages = "2846-2837",
number = "7",
volume = "30",
doi = "10.1007/s10924-022-02400-w"
}

Ferrero, P., Attallah, O. A., Angel Valera, M., Aleksić, I., Azeem, M., Nikodinović-Runić, J.,& Fournet, M. B.. (2022). Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation. in Journal of Polymers and the Environment
Springer, New York., 30(7), 2837-2846.
https://doi.org/10.1007/s10924-022-02400-w

Ferrero P, Attallah OA, Angel Valera M, Aleksić I, Azeem M, Nikodinović-Runić J, Fournet MB. Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation. in Journal of Polymers and the Environment. 2022;30(7):2837-2846.
doi:10.1007/s10924-022-02400-w .

Ferrero, Pablo, Attallah, Olivia A., Angel Valera, Miguel, Aleksić, Ivana, Azeem, Muhammad, Nikodinović-Runić, Jasmina, Fournet, Margaret Brennan, "Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation" in Journal of Polymers and the Environment, 30, no. 7 (2022):2837-2846,
https://doi.org/10.1007/s10924-022-02400-w . .

TY  - JOUR
AU  - Lazić, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Vojnović, Sandra
AU  - Aleksić, Ivana
AU  - Milivojević, Dušan
AU  - Kretzschmar, Martin
AU  - Gulder, Tanja
AU  - Petković, Milos
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1591
AB  - Prodigiosins (prodiginines) are a class of bacterial secondary metabolites with remarkable biological activities and color. In this study, optimized production, purification, and characterization of prodigiosin (PG) from easily accessible Serratia marcescens ATCC 27117 strain has been achieved to levels of 14 mg/L of culture within 24 h. Furthermore, environmentally friendly bromination of produced PG was used to afford both novel mono- and dibrominated derivatives of PG. PG and its Br derivatives showed anticancer potential with IC50 values range 0.62-17.00 mu g/mL for all tested cancer cell lines and induction of apoptosis but low selectivity against healthy cell lines. All compounds did not affect Caenorhabditis elegans at concentrations up to 50 mu g/mL. However, an improved toxicity profile of Br derivatives in comparison to parent PG was observed in vivo using zebrafish (Danio rerio) model system, when 10 mu g/mL applied at 6 h post fertilization caused death rate of 100%, 30% and 0% by PG, PG-Br, and PG-Br-2,Br- respectively, which is a significant finding for further structural optimizations of bacterial prodigiosins. The drug-likeness of PG and its Br derivatives was examined, and the novel Br derivatives obey the Lipinski's "rule of five", with an exemption of being more lipophilic than PG, which still makes them good targets for further structural optimization.
PB  - MDPI, Basel
T2  - Molecules
T1  - Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117
IS  - 12
VL  - 27
DO  - 10.3390/molecules27123729
ER  - 

@article{
author = "Lazić, Jelena and Škaro Bogojević, Sanja and Vojnović, Sandra and Aleksić, Ivana and Milivojević, Dušan and Kretzschmar, Martin and Gulder, Tanja and Petković, Milos and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Prodigiosins (prodiginines) are a class of bacterial secondary metabolites with remarkable biological activities and color. In this study, optimized production, purification, and characterization of prodigiosin (PG) from easily accessible Serratia marcescens ATCC 27117 strain has been achieved to levels of 14 mg/L of culture within 24 h. Furthermore, environmentally friendly bromination of produced PG was used to afford both novel mono- and dibrominated derivatives of PG. PG and its Br derivatives showed anticancer potential with IC50 values range 0.62-17.00 mu g/mL for all tested cancer cell lines and induction of apoptosis but low selectivity against healthy cell lines. All compounds did not affect Caenorhabditis elegans at concentrations up to 50 mu g/mL. However, an improved toxicity profile of Br derivatives in comparison to parent PG was observed in vivo using zebrafish (Danio rerio) model system, when 10 mu g/mL applied at 6 h post fertilization caused death rate of 100%, 30% and 0% by PG, PG-Br, and PG-Br-2,Br- respectively, which is a significant finding for further structural optimizations of bacterial prodigiosins. The drug-likeness of PG and its Br derivatives was examined, and the novel Br derivatives obey the Lipinski's "rule of five", with an exemption of being more lipophilic than PG, which still makes them good targets for further structural optimization.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117",
number = "12",
volume = "27",
doi = "10.3390/molecules27123729"
}

Lazić, J., Škaro Bogojević, S., Vojnović, S., Aleksić, I., Milivojević, D., Kretzschmar, M., Gulder, T., Petković, M.,& Nikodinović-Runić, J.. (2022). Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117. in Molecules
MDPI, Basel., 27(12).
https://doi.org/10.3390/molecules27123729

Lazić J, Škaro Bogojević S, Vojnović S, Aleksić I, Milivojević D, Kretzschmar M, Gulder T, Petković M, Nikodinović-Runić J. Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117. in Molecules. 2022;27(12).
doi:10.3390/molecules27123729 .

Lazić, Jelena, Škaro Bogojević, Sanja, Vojnović, Sandra, Aleksić, Ivana, Milivojević, Dušan, Kretzschmar, Martin, Gulder, Tanja, Petković, Milos, Nikodinović-Runić, Jasmina, "Synthesis, Anticancer Potential and Comprehensive Toxicity Studies of Novel Brominated Derivatives of Bacterial Biopigment Prodigiosin from Serratia marcescens ATCC 27117" in Molecules, 27, no. 12 (2022),
https://doi.org/10.3390/molecules27123729 . .

TY  - JOUR
AU  - Andrejević, Tina P.
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Pantović, Bojana, V
AU  - Milivojević, Dušan
AU  - Vojnović, Sandra
AU  - Turel, Iztok
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1589
AB  - Two zinc(II) complexes with dimethyl 2,2 '-bipyridine-4,5-dicarboxylate (py-2py) of the general formula [Zn(py-2py)X-2], X = Cl- (1) and Br- (2) were synthesized and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. Complexes 1 and 2 are isostructural and adopt a slightly distorted tetrahedral geometry with values of tetrahedral indices tau(4) and tau'(4) in the range of 0.80-0.85. The complexes were evaluated for their in vitro antimicrobial activity against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two fungal strains (Candida albicans and Candida parapsilosis), while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5) and the model organism Caenorhabditis elegans. Complex 1 showed moderate activity against both Candida strains. However, this complex was twofold more cytotoxic compared to complex 2. The complexes tested had no effect on the survival rate of C. elegans. Complex 2 showed the ability to inhibit filamentation of C. albicans, while complex 1 was more effective than complex 2 in inhibiting biofilm formation. The interactions of complexes 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) were studied to evaluate their binding affinity toward these biomolecules.
PB  - MDPI, Basel
T2  - Inorganics
T1  - Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study
IS  - 6
VL  - 10
DO  - 10.3390/inorganics10060071
ER  - 

@article{
author = "Andrejević, Tina P. and Aleksić, Ivana and Kljun, Jakob and Pantović, Bojana, V and Milivojević, Dušan and Vojnović, Sandra and Turel, Iztok and Djuran, Milos and Glišić, Biljana",
year = "2022",
abstract = "Two zinc(II) complexes with dimethyl 2,2 '-bipyridine-4,5-dicarboxylate (py-2py) of the general formula [Zn(py-2py)X-2], X = Cl- (1) and Br- (2) were synthesized and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. Complexes 1 and 2 are isostructural and adopt a slightly distorted tetrahedral geometry with values of tetrahedral indices tau(4) and tau'(4) in the range of 0.80-0.85. The complexes were evaluated for their in vitro antimicrobial activity against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two fungal strains (Candida albicans and Candida parapsilosis), while their cytotoxicity was tested on the normal human lung fibroblast cell line (MRC-5) and the model organism Caenorhabditis elegans. Complex 1 showed moderate activity against both Candida strains. However, this complex was twofold more cytotoxic compared to complex 2. The complexes tested had no effect on the survival rate of C. elegans. Complex 2 showed the ability to inhibit filamentation of C. albicans, while complex 1 was more effective than complex 2 in inhibiting biofilm formation. The interactions of complexes 1 and 2 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) were studied to evaluate their binding affinity toward these biomolecules.",
publisher = "MDPI, Basel",
journal = "Inorganics",
title = "Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study",
number = "6",
volume = "10",
doi = "10.3390/inorganics10060071"
}

Andrejević, T. P., Aleksić, I., Kljun, J., Pantović, B. V., Milivojević, D., Vojnović, S., Turel, I., Djuran, M.,& Glišić, B.. (2022). Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study. in Inorganics
MDPI, Basel., 10(6).
https://doi.org/10.3390/inorganics10060071

Andrejević TP, Aleksić I, Kljun J, Pantović BV, Milivojević D, Vojnović S, Turel I, Djuran M, Glišić B. Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study. in Inorganics. 2022;10(6).
doi:10.3390/inorganics10060071 .

Andrejević, Tina P., Aleksić, Ivana, Kljun, Jakob, Pantović, Bojana, V, Milivojević, Dušan, Vojnović, Sandra, Turel, Iztok, Djuran, Milos, Glišić, Biljana, "Zinc(II) Complexes with Dimethyl 2,2 '-Bipyridine-4,5-dicarboxylate: Structure, Antimicrobial Activity and DNA/BSA Binding Study" in Inorganics, 10, no. 6 (2022),
https://doi.org/10.3390/inorganics10060071 . .

TY  - JOUR
AU  - Stevanović, Nevena Lj.
AU  - Kljun, Jakob
AU  - Aleksić, Ivana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Veselinović, Aleksandar
AU  - Turel, Iztok
AU  - Djuran, Milos
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić, Biljana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1568
AB  - In a search for novel antimicrobial metal-based therapeutic agents, mononuclear gold(III) complexes 1-7 of the general formula [AuCl3(azole)], where azole stands for imidazole (im, 1), 1-isopropylimidazole (ipim, 2), 1-phenylimidazole (phim, 3), clotrimazole (ctz, 4), econazole (ecz, 5), tioconazole (tcz, 6) and voriconazole (vcz, 7) were synthesized, characterized and biologically evaluated. In all complexes, the corresponding azole ligand is monodentately coordinated to the Au(III) via the imidazole or triazole nitrogen atom, while the remaining coordination sites are occupied by chloride anions leading to the square-planar arrangement. In vitro antimicrobial assays showed that the complexation of inactive azoles, imidazole, 1-isopropylimidazole and 1-phenylimidazole, to the Au(III) ion led to complexes 1-3, respectively, with moderate activity against the investigated strains and low cytotoxicity on the human normal lung fibroblast cell line (MRC-5). Moreover, gold(III) complexes 4-7 with clinically used antifungal agents clotrimazole, econazole, tioconazole and voriconazole, respectively, have, in most cases, enhanced antimicrobial effectiveness relative to the corresponding azoles, with the best improvement achieved after complexation of tioconazole (6) and voriconazole (7). The complexes 4-7 and the corresponding antifungal azoles inhibited the growth of dermatophyte Microsporum canis at 50 and 25 mu g mL(-1). Gold(III) complexes 1-3 significantly reduced the amount of ergosterol in the cell membrane of Candida albicans at the subinhibitory concentration of 0.5 x MIC (minimal inhibitory concentration), while the corresponding imidazole ligands did not significantly affect the ergosterol content, indicating that the mechanism of action of the gold(III)-azole complexes is associated with inhibition of ergosterol biosynthesis. Finally, complexes 5 and 6 significantly reduced the production of pyocyanin, a virulence factor in Pseudomonas aeruginosa controlled by quorum sensing, and increased cell survival after exposure to this bacterium. These findings could be of importance for the development of novel gold(III)-based antivirulence therapeutic agents that attenuate virulence without pronounced effect on the growth of the pathogens, offering a lower risk for resistance development.
PB  - Royal Soc Chemistry, Cambridge
T2  - Dalton Transactions
T1  - Clinically used antifungal azoles as ligands for gold(III) complexes: the influence of the Au(III) ion on the antimicrobial activity of the complex
EP  - 5334
IS  - 13
SP  - 5322
VL  - 51
DO  - 10.1039/d2dt00411a
ER  - 

@article{
author = "Stevanović, Nevena Lj. and Kljun, Jakob and Aleksić, Ivana and Škaro Bogojević, Sanja and Milivojević, Dušan and Veselinović, Aleksandar and Turel, Iztok and Djuran, Milos and Nikodinović-Runić, Jasmina and Glišić, Biljana",
year = "2022",
abstract = "In a search for novel antimicrobial metal-based therapeutic agents, mononuclear gold(III) complexes 1-7 of the general formula [AuCl3(azole)], where azole stands for imidazole (im, 1), 1-isopropylimidazole (ipim, 2), 1-phenylimidazole (phim, 3), clotrimazole (ctz, 4), econazole (ecz, 5), tioconazole (tcz, 6) and voriconazole (vcz, 7) were synthesized, characterized and biologically evaluated. In all complexes, the corresponding azole ligand is monodentately coordinated to the Au(III) via the imidazole or triazole nitrogen atom, while the remaining coordination sites are occupied by chloride anions leading to the square-planar arrangement. In vitro antimicrobial assays showed that the complexation of inactive azoles, imidazole, 1-isopropylimidazole and 1-phenylimidazole, to the Au(III) ion led to complexes 1-3, respectively, with moderate activity against the investigated strains and low cytotoxicity on the human normal lung fibroblast cell line (MRC-5). Moreover, gold(III) complexes 4-7 with clinically used antifungal agents clotrimazole, econazole, tioconazole and voriconazole, respectively, have, in most cases, enhanced antimicrobial effectiveness relative to the corresponding azoles, with the best improvement achieved after complexation of tioconazole (6) and voriconazole (7). The complexes 4-7 and the corresponding antifungal azoles inhibited the growth of dermatophyte Microsporum canis at 50 and 25 mu g mL(-1). Gold(III) complexes 1-3 significantly reduced the amount of ergosterol in the cell membrane of Candida albicans at the subinhibitory concentration of 0.5 x MIC (minimal inhibitory concentration), while the corresponding imidazole ligands did not significantly affect the ergosterol content, indicating that the mechanism of action of the gold(III)-azole complexes is associated with inhibition of ergosterol biosynthesis. Finally, complexes 5 and 6 significantly reduced the production of pyocyanin, a virulence factor in Pseudomonas aeruginosa controlled by quorum sensing, and increased cell survival after exposure to this bacterium. These findings could be of importance for the development of novel gold(III)-based antivirulence therapeutic agents that attenuate virulence without pronounced effect on the growth of the pathogens, offering a lower risk for resistance development.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Dalton Transactions",
title = "Clinically used antifungal azoles as ligands for gold(III) complexes: the influence of the Au(III) ion on the antimicrobial activity of the complex",
pages = "5334-5322",
number = "13",
volume = "51",
doi = "10.1039/d2dt00411a"
}

Stevanović, N. Lj., Kljun, J., Aleksić, I., Škaro Bogojević, S., Milivojević, D., Veselinović, A., Turel, I., Djuran, M., Nikodinović-Runić, J.,& Glišić, B.. (2022). Clinically used antifungal azoles as ligands for gold(III) complexes: the influence of the Au(III) ion on the antimicrobial activity of the complex. in Dalton Transactions
Royal Soc Chemistry, Cambridge., 51(13), 5322-5334.
https://doi.org/10.1039/d2dt00411a

Stevanović NL, Kljun J, Aleksić I, Škaro Bogojević S, Milivojević D, Veselinović A, Turel I, Djuran M, Nikodinović-Runić J, Glišić B. Clinically used antifungal azoles as ligands for gold(III) complexes: the influence of the Au(III) ion on the antimicrobial activity of the complex. in Dalton Transactions. 2022;51(13):5322-5334.
doi:10.1039/d2dt00411a .

Stevanović, Nevena Lj., Kljun, Jakob, Aleksić, Ivana, Škaro Bogojević, Sanja, Milivojević, Dušan, Veselinović, Aleksandar, Turel, Iztok, Djuran, Milos, Nikodinović-Runić, Jasmina, Glišić, Biljana, "Clinically used antifungal azoles as ligands for gold(III) complexes: the influence of the Au(III) ion on the antimicrobial activity of the complex" in Dalton Transactions, 51, no. 13 (2022):5322-5334,
https://doi.org/10.1039/d2dt00411a . .

TY  - JOUR
AU  - Keskin, Selbi
AU  - Dogan, Sengul Dilem
AU  - Gunduz, Miyase Gozde
AU  - Aleksić, Ivana
AU  - Vojnović, Sandra
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1514
AB  - In the present study, we report the synthesis, cytotoxicity determination and molecular modeling studies of twelve novel indole-based hydrazone derivatives ( IH1 - IH12 ). To obtain the target molecules, initially, 1 H -indole-2-carboxylic acid and ethanol were heated in the presence of an acid catalyst to yield ethyl 1 H -indole-2-carboxylate. Following the hydrazinolyzation of the ester moiety, the resulting compound, 1 H -indole-2-carbohydrazide reacted with appropriate benzaldehyde derivatives to obtain IH1 - IH12 . The proposed chemical structures of all compounds were confirmed by their 1 H NMR, 13 C NMR, IR, and HRMS data. Additionally, the configuration of C = N bond in IH8 was determined as ( E ) by applying 2D NMR technique, NOESY. Subsequently, the compounds were tested against both colon cancer (HCT116) and lung cancer (A549), as well as healthy lung fibroblast (MRC-5) cell lines to determine their potential as anticancer agents and their selectivity indexes (SI). Based on the obtained data from the antiproliferative MTT assay, HCT116 cell line was more sensitive to our molecules compared to A549. Furthermore, lipophilic halogens were preferable substituents on the phenyl ring for the selective toxicity against cancer cell lines. Drug-likeness analysis carried out by calculating important physicochemical properties of IH1 - IH12 confirmed that they all obey Lipinski's rule of five. Finally, hypoxia inducible factor (HIF)-1 alpha was suggested as the potential biological target of the compounds through molecular docking studies.
PB  - Elsevier, Amsterdam
T2  - Journal of Molecular Structure
T1  - Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies
VL  - 1270
DO  - 10.1016/j.molstruc.2022.133936
ER  - 

@article{
author = "Keskin, Selbi and Dogan, Sengul Dilem and Gunduz, Miyase Gozde and Aleksić, Ivana and Vojnović, Sandra and Lazić, Jelena and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "In the present study, we report the synthesis, cytotoxicity determination and molecular modeling studies of twelve novel indole-based hydrazone derivatives ( IH1 - IH12 ). To obtain the target molecules, initially, 1 H -indole-2-carboxylic acid and ethanol were heated in the presence of an acid catalyst to yield ethyl 1 H -indole-2-carboxylate. Following the hydrazinolyzation of the ester moiety, the resulting compound, 1 H -indole-2-carbohydrazide reacted with appropriate benzaldehyde derivatives to obtain IH1 - IH12 . The proposed chemical structures of all compounds were confirmed by their 1 H NMR, 13 C NMR, IR, and HRMS data. Additionally, the configuration of C = N bond in IH8 was determined as ( E ) by applying 2D NMR technique, NOESY. Subsequently, the compounds were tested against both colon cancer (HCT116) and lung cancer (A549), as well as healthy lung fibroblast (MRC-5) cell lines to determine their potential as anticancer agents and their selectivity indexes (SI). Based on the obtained data from the antiproliferative MTT assay, HCT116 cell line was more sensitive to our molecules compared to A549. Furthermore, lipophilic halogens were preferable substituents on the phenyl ring for the selective toxicity against cancer cell lines. Drug-likeness analysis carried out by calculating important physicochemical properties of IH1 - IH12 confirmed that they all obey Lipinski's rule of five. Finally, hypoxia inducible factor (HIF)-1 alpha was suggested as the potential biological target of the compounds through molecular docking studies.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Molecular Structure",
title = "Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies",
volume = "1270",
doi = "10.1016/j.molstruc.2022.133936"
}

Keskin, S., Dogan, S. D., Gunduz, M. G., Aleksić, I., Vojnović, S., Lazić, J.,& Nikodinović-Runić, J.. (2022). Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies. in Journal of Molecular Structure
Elsevier, Amsterdam., 1270.
https://doi.org/10.1016/j.molstruc.2022.133936

Keskin S, Dogan SD, Gunduz MG, Aleksić I, Vojnović S, Lazić J, Nikodinović-Runić J. Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies. in Journal of Molecular Structure. 2022;1270.
doi:10.1016/j.molstruc.2022.133936 .

Keskin, Selbi, Dogan, Sengul Dilem, Gunduz, Miyase Gozde, Aleksić, Ivana, Vojnović, Sandra, Lazić, Jelena, Nikodinović-Runić, Jasmina, "Indole-based hydrazone derivatives: Synthesis, cytotoxicity assessment, and molecular modeling studies" in Journal of Molecular Structure, 1270 (2022),
https://doi.org/10.1016/j.molstruc.2022.133936 . .

TY  - JOUR
AU  - Ferrero, Pablo
AU  - Attallah, Olivia A.
AU  - Angel Valera, Miguel
AU  - Aleksić, Ivana
AU  - Azeem, Muhammad
AU  - Nikodinović-Runić, Jasmina
AU  - Fournet, Margaret Brennan
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1575
AB  - An energy-efficient high throughput pre-treatment of low-density polyethylene (LDPE) using a fast, reactive extrusion (REX) assisted oxidation technique followed by bacterial attachment as an indicator for bio-amenability was studied. Silicon dioxide (SiO2) was selected as a model oxidizing and catalytic reagent with the REX process demonstrated to be effective both in the presence and absence of the catalyst. Optimized 5-min duration pre-treatment conditions were determined using Box-Behnken design (BBD) with respect to screws speed, operating temperature, and concentration of SiO2. The crystallinity index, carbonyl index and weight loss (%) of LDPE were used as the studied responses for BDD. FTIR and DSC spectra of the residual LDPE obtained after pre-treatment with the REX assisted oxidation technique showed a significant increase in residual LDPE carbonyl index from 0 to 1.04 and a decrease of LDPE crystallinity index from 29 to 18%. Up to fivefold molecular weight reductions were also demonstrated using gel permeation chromatography. Optimum LDPE pre-treatment with a duration of 5 min was obtained at low screw speed (50 rpm), operating temperature of 380-390 degrees C and variable concentration of SiO2 (0 and 2% (w/w)) indicating that effective pre-treatment can occur under noncatalytic and catalysed conditions. Biofilms were successfully formed on pre-treated LDPE samples after 14 days of incubation. Furthermore, the technique proposed in this study is expected to provide a high throughput approach for pre-treatment of pervasive recalcitrant PE-based plastics to reduce their bio inertness.
PB  - Springer, New York
T2  - Journal of Polymers and the Environment
T1  - Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation
EP  - 2846
IS  - 7
SP  - 2837
VL  - 30
DO  - 10.1007/s10924-022-02400-w
ER  - 

@article{
author = "Ferrero, Pablo and Attallah, Olivia A. and Angel Valera, Miguel and Aleksić, Ivana and Azeem, Muhammad and Nikodinović-Runić, Jasmina and Fournet, Margaret Brennan",
year = "2022",
abstract = "An energy-efficient high throughput pre-treatment of low-density polyethylene (LDPE) using a fast, reactive extrusion (REX) assisted oxidation technique followed by bacterial attachment as an indicator for bio-amenability was studied. Silicon dioxide (SiO2) was selected as a model oxidizing and catalytic reagent with the REX process demonstrated to be effective both in the presence and absence of the catalyst. Optimized 5-min duration pre-treatment conditions were determined using Box-Behnken design (BBD) with respect to screws speed, operating temperature, and concentration of SiO2. The crystallinity index, carbonyl index and weight loss (%) of LDPE were used as the studied responses for BDD. FTIR and DSC spectra of the residual LDPE obtained after pre-treatment with the REX assisted oxidation technique showed a significant increase in residual LDPE carbonyl index from 0 to 1.04 and a decrease of LDPE crystallinity index from 29 to 18%. Up to fivefold molecular weight reductions were also demonstrated using gel permeation chromatography. Optimum LDPE pre-treatment with a duration of 5 min was obtained at low screw speed (50 rpm), operating temperature of 380-390 degrees C and variable concentration of SiO2 (0 and 2% (w/w)) indicating that effective pre-treatment can occur under noncatalytic and catalysed conditions. Biofilms were successfully formed on pre-treated LDPE samples after 14 days of incubation. Furthermore, the technique proposed in this study is expected to provide a high throughput approach for pre-treatment of pervasive recalcitrant PE-based plastics to reduce their bio inertness.",
publisher = "Springer, New York",
journal = "Journal of Polymers and the Environment",
title = "Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation",
pages = "2846-2837",
number = "7",
volume = "30",
doi = "10.1007/s10924-022-02400-w"
}

Ferrero, P., Attallah, O. A., Angel Valera, M., Aleksić, I., Azeem, M., Nikodinović-Runić, J.,& Fournet, M. B.. (2022). Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation. in Journal of Polymers and the Environment
Springer, New York., 30(7), 2837-2846.
https://doi.org/10.1007/s10924-022-02400-w

Ferrero P, Attallah OA, Angel Valera M, Aleksić I, Azeem M, Nikodinović-Runić J, Fournet MB. Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation. in Journal of Polymers and the Environment. 2022;30(7):2837-2846.
doi:10.1007/s10924-022-02400-w .

Ferrero, Pablo, Attallah, Olivia A., Angel Valera, Miguel, Aleksić, Ivana, Azeem, Muhammad, Nikodinović-Runić, Jasmina, Fournet, Margaret Brennan, "Rendering Bio-inert Low-Density Polyethylene Amenable for Biodegradation via Fast High Throughput Reactive Extrusion Assisted Oxidation" in Journal of Polymers and the Environment, 30, no. 7 (2022):2837-2846,
https://doi.org/10.1007/s10924-022-02400-w . .

TY  - JOUR
AU  - Stevanović, Nevena Lj.
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Škaro Bogojević, Sanja
AU  - Veselinović, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Milos 
AU  - Glišić, Biljana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1484
AB  - Copper(II) and zinc(II) complexes with clinically used antifungal drug fluconazole (fcz), {[CuCl2(fcz)(2)](.)5H(2)O}(n), 1, and {[ZnCl2(fcz)(2)]Greek ano teleia2C(2)H(5)OH}(n), 2, were prepared and characterized by spectroscopic and crystallographic methods. The polymeric structure of the complexes comprises four fluconazole molecules monodentately coordinated via the triazole nitrogen and two chlorido ligands. With respect to fluconazole, complex 2 showed significantly higher antifungal activity against Candida krusei and Candida parapsilosis. All tested compounds reduced the total amount of ergosterol at subinhibitory concentrations, indicating that the mode of activity of fluconazole was retained within the complexes, which was corroborated via molecular docking with cytochrome P450 sterol 14 alpha-demethylase (CYP51) as a target. Electrostatic, steric and internal energy interactions between the complexes and enzyme showed that 2 has higher binding potency to this target. Both complexes showed strong inhibition of C. albicans filamentation and biofilm formation at subinhibitory concentrations, with 2 being able to reduce the adherence of C. albicans to A549 cells in vitro. Complex 2 was able to reduce pyocyanin production in Pseudomonas aeruginosa between 10% and 25% and to inhibit its biofilm formation by 20% in comparison to the untreated control. These results suggest that complex 2 may be further examined in the mixed Candida-P. aeruginosa infections.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Copper(II) and Zinc(II) Complexes with the Clinically Used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential
IS  - 1
VL  - 14
DO  - 10.3390/ph14010024
ER  - 

@article{
author = "Stevanović, Nevena Lj. and Aleksić, Ivana and Kljun, Jakob and Škaro Bogojević, Sanja and Veselinović, Aleksandar and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Milos  and Glišić, Biljana",
year = "2021",
abstract = "Copper(II) and zinc(II) complexes with clinically used antifungal drug fluconazole (fcz), {[CuCl2(fcz)(2)](.)5H(2)O}(n), 1, and {[ZnCl2(fcz)(2)]Greek ano teleia2C(2)H(5)OH}(n), 2, were prepared and characterized by spectroscopic and crystallographic methods. The polymeric structure of the complexes comprises four fluconazole molecules monodentately coordinated via the triazole nitrogen and two chlorido ligands. With respect to fluconazole, complex 2 showed significantly higher antifungal activity against Candida krusei and Candida parapsilosis. All tested compounds reduced the total amount of ergosterol at subinhibitory concentrations, indicating that the mode of activity of fluconazole was retained within the complexes, which was corroborated via molecular docking with cytochrome P450 sterol 14 alpha-demethylase (CYP51) as a target. Electrostatic, steric and internal energy interactions between the complexes and enzyme showed that 2 has higher binding potency to this target. Both complexes showed strong inhibition of C. albicans filamentation and biofilm formation at subinhibitory concentrations, with 2 being able to reduce the adherence of C. albicans to A549 cells in vitro. Complex 2 was able to reduce pyocyanin production in Pseudomonas aeruginosa between 10% and 25% and to inhibit its biofilm formation by 20% in comparison to the untreated control. These results suggest that complex 2 may be further examined in the mixed Candida-P. aeruginosa infections.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Copper(II) and Zinc(II) Complexes with the Clinically Used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential",
number = "1",
volume = "14",
doi = "10.3390/ph14010024"
}

Stevanović, N. Lj., Aleksić, I., Kljun, J., Škaro Bogojević, S., Veselinović, A., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2021). Copper(II) and Zinc(II) Complexes with the Clinically Used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential. in Pharmaceuticals
MDPI, Basel., 14(1).
https://doi.org/10.3390/ph14010024

Stevanović NL, Aleksić I, Kljun J, Škaro Bogojević S, Veselinović A, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Copper(II) and Zinc(II) Complexes with the Clinically Used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential. in Pharmaceuticals. 2021;14(1).
doi:10.3390/ph14010024 .

Stevanović, Nevena Lj., Aleksić, Ivana, Kljun, Jakob, Škaro Bogojević, Sanja, Veselinović, Aleksandar, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Milos , Glišić, Biljana, "Copper(II) and Zinc(II) Complexes with the Clinically Used Fluconazole: Comparison of Antifungal Activity and Therapeutic Potential" in Pharmaceuticals, 14, no. 1 (2021),
https://doi.org/10.3390/ph14010024 . .

TY  - JOUR
AU  - Andrejević, Tina P.
AU  - Aleksić, Ivana
AU  - Pockaj, Marta
AU  - Kljun, Jakob
AU  - Milivojević, Dušan
AU  - Stevanović, Nevena Lj.
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Milos
AU  - Glišić, Biljana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1492
AB  - Five novel copper(ii) complexes with pyridine-4,5-dicarboxylate esters as ligands, [Cu(NO3)(py-2tz)(H2O)(3)]NO3 (1), [Cu(NO3)(2)(py-2metz)(H2O)] (2), [Cu(NO3)(2)(py-2py)(H2O)]center dot H2O (3), [CuCl2(py-2tz)](2) (4) and [CuCl2(py-2metz)](n) (5) (py-2tz is dimethyl 2-(thiazol-2-yl)pyridine-4,5-dicarboxylate, py-2metz is dimethyl 2-(4-methylthiazol-2-yl)pyridine-4,5-dicarboxylate and py-2py is dimethyl 2,2 '-bipyridine-4,5-dicarboxylate), were synthesized and structurally characterized by different spectroscopic and electrochemical methods. The structure of these complexes was determined by single-crystal X-ray diffraction analysis, confirming the bidentate coordination mode of the corresponding pyridine-4,5-dicarboxylate ester to the Cu(ii) ion through the nitrogen atoms. The antimicrobial potential of copper(ii) complexes 1-5 was assessed against two bacterial and two Candida species. These complexes showed better growth inhibiting activity against Candida spp. with respect to the tested bacterial species, also being moderately toxic towards normal human lung fibroblast cells (MRC-5). Complexes 1 and 4 showed the greatest ability to inhibit the filamentation of C. albicans, which is an important process during fungal infection, and these two complexes efficiently inhibited the biofilm formation of C. albicans at subinhibitory concentrations. Complex 4 also successfully prevented the adhesion of C. albicans in an in vitro epithelial cell model. The mechanism of the antifungal activity of copper(ii) complexes 1-5 was studied through their interaction with ct-DNA, as one of the possible target biomolecules, by fluorescence spectroscopy and gel electrophoresis. Finally, the ability of these complexes to bind to bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy.
PB  - Royal Soc Chemistry, Cambridge
T2  - Dalton Transactions
T1  - Tailoring copper(II) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity
EP  - 2638
IS  - 7
SP  - 2627
VL  - 50
DO  - 10.1039/d0dt04061d
ER  - 

@article{
author = "Andrejević, Tina P. and Aleksić, Ivana and Pockaj, Marta and Kljun, Jakob and Milivojević, Dušan and Stevanović, Nevena Lj. and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Milos and Glišić, Biljana",
year = "2021",
abstract = "Five novel copper(ii) complexes with pyridine-4,5-dicarboxylate esters as ligands, [Cu(NO3)(py-2tz)(H2O)(3)]NO3 (1), [Cu(NO3)(2)(py-2metz)(H2O)] (2), [Cu(NO3)(2)(py-2py)(H2O)]center dot H2O (3), [CuCl2(py-2tz)](2) (4) and [CuCl2(py-2metz)](n) (5) (py-2tz is dimethyl 2-(thiazol-2-yl)pyridine-4,5-dicarboxylate, py-2metz is dimethyl 2-(4-methylthiazol-2-yl)pyridine-4,5-dicarboxylate and py-2py is dimethyl 2,2 '-bipyridine-4,5-dicarboxylate), were synthesized and structurally characterized by different spectroscopic and electrochemical methods. The structure of these complexes was determined by single-crystal X-ray diffraction analysis, confirming the bidentate coordination mode of the corresponding pyridine-4,5-dicarboxylate ester to the Cu(ii) ion through the nitrogen atoms. The antimicrobial potential of copper(ii) complexes 1-5 was assessed against two bacterial and two Candida species. These complexes showed better growth inhibiting activity against Candida spp. with respect to the tested bacterial species, also being moderately toxic towards normal human lung fibroblast cells (MRC-5). Complexes 1 and 4 showed the greatest ability to inhibit the filamentation of C. albicans, which is an important process during fungal infection, and these two complexes efficiently inhibited the biofilm formation of C. albicans at subinhibitory concentrations. Complex 4 also successfully prevented the adhesion of C. albicans in an in vitro epithelial cell model. The mechanism of the antifungal activity of copper(ii) complexes 1-5 was studied through their interaction with ct-DNA, as one of the possible target biomolecules, by fluorescence spectroscopy and gel electrophoresis. Finally, the ability of these complexes to bind to bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Dalton Transactions",
title = "Tailoring copper(II) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity",
pages = "2638-2627",
number = "7",
volume = "50",
doi = "10.1039/d0dt04061d"
}

Andrejević, T. P., Aleksić, I., Pockaj, M., Kljun, J., Milivojević, D., Stevanović, N. Lj., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2021). Tailoring copper(II) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity. in Dalton Transactions
Royal Soc Chemistry, Cambridge., 50(7), 2627-2638.
https://doi.org/10.1039/d0dt04061d

Andrejević TP, Aleksić I, Pockaj M, Kljun J, Milivojević D, Stevanović NL, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Tailoring copper(II) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity. in Dalton Transactions. 2021;50(7):2627-2638.
doi:10.1039/d0dt04061d .

Andrejević, Tina P., Aleksić, Ivana, Pockaj, Marta, Kljun, Jakob, Milivojević, Dušan, Stevanović, Nevena Lj., Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Milos, Glišić, Biljana, "Tailoring copper(II) complexes with pyridine-4,5-dicarboxylate esters for anti-Candida activity" in Dalton Transactions, 50, no. 7 (2021):2627-2638,
https://doi.org/10.1039/d0dt04061d . .

TY  - JOUR
AU  - Ašanin, Darko P.
AU  - Škaro Bogojević, Sanja
AU  - Perdih, Franc
AU  - Andrejević, Tina P.
AU  - Milivojević, Dušan
AU  - Aleksić, Ivana
AU  - Nikodinović-Runić, Jasmina
AU  - Glišić, Biljana
AU  - Turel, Iztok
AU  - Djuran, Milos
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1420
AB  - Three new silver(I) complexes [Ag(NO3)(tia)(H2O)](n) (Ag1), [Ag(CF3SO3)(1,8-naph)](n) (Ag2) and [Ag-2(1,8-naph)(2)(H2O)(1.2)](PF6)(2) (Ag3), where tia is thianthrene and 1,8-naph is 1,8-naphthyridine, were synthesized and structurally characterized by different spectroscopic and electrochemical methods and their crystal structures were determined by single-crystal X-ray diffraction analysis. Their antimicrobial potential was evaluated against four bacterial and three Candida species, and the obtained results revealed that these complexes showed significant activity toward the Gram-positive Staphylococcus aureus, Gram-negative Pseudomonas aeruginosa and the investigated Candida species with minimal inhibitory concentration (MIC) values in the range 1.56-7.81 mu g/mL. On the other hand, tia and 1,8-naph ligands were not active against the investigated strains, suggesting that their complexation with Ag(I) ion results in the formation of antimicrobial compounds. Moreover, low toxicity of the complexes was detected by in vivo model Caenorhabditis elegans. The interaction of the complexes with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied to evaluate their binding affinity towards these biomolecules for possible insights into the mode of antimicrobial activity. The binding affinity of Ag1-3 to BSA was higher than that for DNA, indicating that proteins could be more favorable binding sites for these complexes in comparison to the nucleic acids.
PB  - Basel : MDPI
T2  - Molecules
T1  - Structural Characterization, Antimicrobial Activity and BSA/DNA Binding Affinity of New Silver(I) Complexes with Thianthrene and 1,8-Naphthyridine
IS  - 7
VL  - 26
DO  - 10.3390/molecules26071871
ER  - 

@article{
author = "Ašanin, Darko P. and Škaro Bogojević, Sanja and Perdih, Franc and Andrejević, Tina P. and Milivojević, Dušan and Aleksić, Ivana and Nikodinović-Runić, Jasmina and Glišić, Biljana and Turel, Iztok and Djuran, Milos",
year = "2021",
abstract = "Three new silver(I) complexes [Ag(NO3)(tia)(H2O)](n) (Ag1), [Ag(CF3SO3)(1,8-naph)](n) (Ag2) and [Ag-2(1,8-naph)(2)(H2O)(1.2)](PF6)(2) (Ag3), where tia is thianthrene and 1,8-naph is 1,8-naphthyridine, were synthesized and structurally characterized by different spectroscopic and electrochemical methods and their crystal structures were determined by single-crystal X-ray diffraction analysis. Their antimicrobial potential was evaluated against four bacterial and three Candida species, and the obtained results revealed that these complexes showed significant activity toward the Gram-positive Staphylococcus aureus, Gram-negative Pseudomonas aeruginosa and the investigated Candida species with minimal inhibitory concentration (MIC) values in the range 1.56-7.81 mu g/mL. On the other hand, tia and 1,8-naph ligands were not active against the investigated strains, suggesting that their complexation with Ag(I) ion results in the formation of antimicrobial compounds. Moreover, low toxicity of the complexes was detected by in vivo model Caenorhabditis elegans. The interaction of the complexes with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied to evaluate their binding affinity towards these biomolecules for possible insights into the mode of antimicrobial activity. The binding affinity of Ag1-3 to BSA was higher than that for DNA, indicating that proteins could be more favorable binding sites for these complexes in comparison to the nucleic acids.",
publisher = "Basel : MDPI",
journal = "Molecules",
title = "Structural Characterization, Antimicrobial Activity and BSA/DNA Binding Affinity of New Silver(I) Complexes with Thianthrene and 1,8-Naphthyridine",
number = "7",
volume = "26",
doi = "10.3390/molecules26071871"
}

Ašanin, D. P., Škaro Bogojević, S., Perdih, F., Andrejević, T. P., Milivojević, D., Aleksić, I., Nikodinović-Runić, J., Glišić, B., Turel, I.,& Djuran, M.. (2021). Structural Characterization, Antimicrobial Activity and BSA/DNA Binding Affinity of New Silver(I) Complexes with Thianthrene and 1,8-Naphthyridine. in Molecules
Basel : MDPI., 26(7).
https://doi.org/10.3390/molecules26071871

Ašanin DP, Škaro Bogojević S, Perdih F, Andrejević TP, Milivojević D, Aleksić I, Nikodinović-Runić J, Glišić B, Turel I, Djuran M. Structural Characterization, Antimicrobial Activity and BSA/DNA Binding Affinity of New Silver(I) Complexes with Thianthrene and 1,8-Naphthyridine. in Molecules. 2021;26(7).
doi:10.3390/molecules26071871 .

Ašanin, Darko P., Škaro Bogojević, Sanja, Perdih, Franc, Andrejević, Tina P., Milivojević, Dušan, Aleksić, Ivana, Nikodinović-Runić, Jasmina, Glišić, Biljana, Turel, Iztok, Djuran, Milos, "Structural Characterization, Antimicrobial Activity and BSA/DNA Binding Affinity of New Silver(I) Complexes with Thianthrene and 1,8-Naphthyridine" in Molecules, 26, no. 7 (2021),
https://doi.org/10.3390/molecules26071871 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Ponjavić, Marijana
AU  - Janković, Vukašin
AU  - Aleksić, Ivana
AU  - Stevanović, Sanja
AU  - Murray, James
AU  - Fournet, Margaret Brennan
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1470
AB  - Meeting the challenge of circularity for plastics requires amenability to repurposing post-use, as equivalent or upcycled products. In a compelling advancement, complete circularity for a biodegradable polyvinyl alcohol/thermoplastic starch (PVA/TPS) food packaging film was demonstrated by bioconversion to high-market-value biopigments and polyhydroxybutyrate (PHB) polyesters. The PVA/TPS film mechanical properties (tensile strength (sigma(u)), 22.2 & PLUSMN; 4.3 MPa; strain at break (epsilon(u)), 325 & PLUSMN; 73%; and Young's modulus (E), 53-250 MPa) compared closely with low-density polyethylene (LDPE) grades used for food packaging. Strong solubility of the PVA/TPS film in water was a pertinent feature, facilitating suitability as a carbon source for bioprocessing and microbial degradation. Biodegradability of the film with greater than 50% weight loss occurred within 30 days of incubation at 37 & DEG;C in a model compost. Up to 22% of the PVA/TPS film substrate conversion to biomass was achieved using three bacterial strains, Ralstonia eutropha H16 (Cupriavidus necator ATCC 17699), Streptomyces sp. JS520, and Bacillus subtilis ATCC6633. For the first time, production of the valuable biopigment (undecylprodigiosin) by Streptomyces sp. JS520 of 5.3 mg/mL and the production of PHB biopolymer at 7.8% of cell dry weight by Ralstonia eutropha H16 from this substrate were reported. This low-energy, low-carbon post-use PVA/TPS film upcycling model approach to plastic circularity demonstrates marked progress in the quest for sustainable and circular plastic solutions.
PB  - MDPI, Basel
T2  - Polymers
T1  - Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer
IS  - 21
VL  - 13
DO  - 10.3390/polym13213692
ER  - 

@article{
author = "Pantelić, Brana and Ponjavić, Marijana and Janković, Vukašin and Aleksić, Ivana and Stevanović, Sanja and Murray, James and Fournet, Margaret Brennan and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Meeting the challenge of circularity for plastics requires amenability to repurposing post-use, as equivalent or upcycled products. In a compelling advancement, complete circularity for a biodegradable polyvinyl alcohol/thermoplastic starch (PVA/TPS) food packaging film was demonstrated by bioconversion to high-market-value biopigments and polyhydroxybutyrate (PHB) polyesters. The PVA/TPS film mechanical properties (tensile strength (sigma(u)), 22.2 & PLUSMN; 4.3 MPa; strain at break (epsilon(u)), 325 & PLUSMN; 73%; and Young's modulus (E), 53-250 MPa) compared closely with low-density polyethylene (LDPE) grades used for food packaging. Strong solubility of the PVA/TPS film in water was a pertinent feature, facilitating suitability as a carbon source for bioprocessing and microbial degradation. Biodegradability of the film with greater than 50% weight loss occurred within 30 days of incubation at 37 & DEG;C in a model compost. Up to 22% of the PVA/TPS film substrate conversion to biomass was achieved using three bacterial strains, Ralstonia eutropha H16 (Cupriavidus necator ATCC 17699), Streptomyces sp. JS520, and Bacillus subtilis ATCC6633. For the first time, production of the valuable biopigment (undecylprodigiosin) by Streptomyces sp. JS520 of 5.3 mg/mL and the production of PHB biopolymer at 7.8% of cell dry weight by Ralstonia eutropha H16 from this substrate were reported. This low-energy, low-carbon post-use PVA/TPS film upcycling model approach to plastic circularity demonstrates marked progress in the quest for sustainable and circular plastic solutions.",
publisher = "MDPI, Basel",
journal = "Polymers",
title = "Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer",
number = "21",
volume = "13",
doi = "10.3390/polym13213692"
}

Pantelić, B., Ponjavić, M., Janković, V., Aleksić, I., Stevanović, S., Murray, J., Fournet, M. B.,& Nikodinović-Runić, J.. (2021). Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer. in Polymers
MDPI, Basel., 13(21).
https://doi.org/10.3390/polym13213692

Pantelić B, Ponjavić M, Janković V, Aleksić I, Stevanović S, Murray J, Fournet MB, Nikodinović-Runić J. Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer. in Polymers. 2021;13(21).
doi:10.3390/polym13213692 .

Pantelić, Brana, Ponjavić, Marijana, Janković, Vukašin, Aleksić, Ivana, Stevanović, Sanja, Murray, James, Fournet, Margaret Brennan, Nikodinović-Runić, Jasmina, "Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer" in Polymers, 13, no. 21 (2021),
https://doi.org/10.3390/polym13213692 . .

TY  - JOUR
AU  - Simić, Milena
AU  - Petković, Milos
AU  - Jovanović, Predrag
AU  - Jovanović, Milos
AU  - Tasić, Gordana
AU  - Besu, Irina
AU  - Zizak, Zeljko
AU  - Aleksić, Ivana
AU  - Nikodinović-Runić, Jasmina
AU  - Savić, Vladimir
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1499
AB  - Several coumarin derivatives with a directly attached azole substituent at C-4 were synthesized and biologically studied for their anticancer properties. The cell lines used for this investigation (HeLa, K-562, MDA-MB-53, and MCF-7) demonstrated different sensitivities. The best response in the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay was shown by K-562 cells, with compounds displaying activity (3c, IC50 3.06 mu M; 4a, IC50 5.24 mu M; 4c, IC50 4.7 mu M) similar to that of cisplatin (IC50 similar to 6 mu M), which was used as the standard. The studied azole-substituted coumarins demonstrated weaker activity toward other cell lines, except for compound 4c, which was equally potent in the case of MCF-7 cells. Additional biological evaluations supported interference with the cell cycle as a potential mechanism of action and confirmed the absence of toxicity in zebrafish embryos. On the basis of these initial results, 4-azole coumarins should be explored further. Although their activity would need additional optimization, the fact that these compounds are fragment-like structures with MW P  lt 3 offers enough flexibility to fine-tune their drug-like properties.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Archiv Der Pharmazie
T1  - Fragment-type 4-azolylcoumarin derivatives with anticancer properties
IS  - 11
VL  - 354
DO  - 10.1002/ardp.202100238
ER  - 

@article{
author = "Simić, Milena and Petković, Milos and Jovanović, Predrag and Jovanović, Milos and Tasić, Gordana and Besu, Irina and Zizak, Zeljko and Aleksić, Ivana and Nikodinović-Runić, Jasmina and Savić, Vladimir",
year = "2021",
abstract = "Several coumarin derivatives with a directly attached azole substituent at C-4 were synthesized and biologically studied for their anticancer properties. The cell lines used for this investigation (HeLa, K-562, MDA-MB-53, and MCF-7) demonstrated different sensitivities. The best response in the MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide) assay was shown by K-562 cells, with compounds displaying activity (3c, IC50 3.06 mu M; 4a, IC50 5.24 mu M; 4c, IC50 4.7 mu M) similar to that of cisplatin (IC50 similar to 6 mu M), which was used as the standard. The studied azole-substituted coumarins demonstrated weaker activity toward other cell lines, except for compound 4c, which was equally potent in the case of MCF-7 cells. Additional biological evaluations supported interference with the cell cycle as a potential mechanism of action and confirmed the absence of toxicity in zebrafish embryos. On the basis of these initial results, 4-azole coumarins should be explored further. Although their activity would need additional optimization, the fact that these compounds are fragment-like structures with MW P  lt 3 offers enough flexibility to fine-tune their drug-like properties.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Archiv Der Pharmazie",
title = "Fragment-type 4-azolylcoumarin derivatives with anticancer properties",
number = "11",
volume = "354",
doi = "10.1002/ardp.202100238"
}

Simić, M., Petković, M., Jovanović, P., Jovanović, M., Tasić, G., Besu, I., Zizak, Z., Aleksić, I., Nikodinović-Runić, J.,& Savić, V.. (2021). Fragment-type 4-azolylcoumarin derivatives with anticancer properties. in Archiv Der Pharmazie
Wiley-V C H Verlag Gmbh, Weinheim., 354(11).
https://doi.org/10.1002/ardp.202100238

Simić M, Petković M, Jovanović P, Jovanović M, Tasić G, Besu I, Zizak Z, Aleksić I, Nikodinović-Runić J, Savić V. Fragment-type 4-azolylcoumarin derivatives with anticancer properties. in Archiv Der Pharmazie. 2021;354(11).
doi:10.1002/ardp.202100238 .

Simić, Milena, Petković, Milos, Jovanović, Predrag, Jovanović, Milos, Tasić, Gordana, Besu, Irina, Zizak, Zeljko, Aleksić, Ivana, Nikodinović-Runić, Jasmina, Savić, Vladimir, "Fragment-type 4-azolylcoumarin derivatives with anticancer properties" in Archiv Der Pharmazie, 354, no. 11 (2021),
https://doi.org/10.1002/ardp.202100238 . .

TY  - CONF
AU  - Stevanović, Nevena
AU  - Aleksic, Ivana
AU  - Kljun, Jakob
AU  - Ašanin, Darko
AU  - Andrejević, Tina
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1641
AB  - Over the last few decades, invasive fungal infections represent a serious problem for modern-day healthcare. Aspergillus, Candida and Cryptococcus species are the most common pathogens causing life-threatening infections. Therapeutic options for the treatment of fungal infections are presently limited to only four classes of compounds and each of these drug classes has significant therapeutic limitations, including serious toxic-side effects, resistance development and limited routes of administration. In order to overcome resistance of the clinically used antifungal triazole agents, we synthesized zinc(II) and copper(II) complexes with fluconazole (flz), {[ZnCl2(flz)2]·2C2H5OH}n (1) and {[CuCl2(flz)2].5H2O}n (2). These complexes were obtained from the reactions between ZnCl2 or CuCl2·2H2O with this antifungal agent in 1 : 2 molar ratio in ethanol at room temperature. The compounds were characterized by elemental analysis, NMR, IR and UV-Vis spectroscopy and mass spectrometry. The crystal structure of complex 1 was determined by a single-crystal X-ray diffraction analysis. The antimicrobial effect of both complexes and fluconazole was evaluated against different Candida species as well as Gram-positive and Gram-negative bacteria by means of minimal inhibitory concentrations (MICs). The obtained results have shown that, in most cases, the coordination of fluconazole to Zn(II) and Cu(II) ions leads to the enhancement of its antifungal activity. Both complexes showed strong inhibitory activity against C. albicans biofilm formation at concentrations lower than MIC values, as well as strong inhibition of C. albicans filamentation.
C3  - Electronic Conference on Medicinal Chemistry
T1  - Improvement of antifungal activity and therapeutic profile of fluconazole by its complexation with copper(II) and zinc(II) ions. Complex characterization and antimicrobial activity studies
DO  - 10.3390/ECMC2020-07373
ER  - 

@conference{
author = "Stevanović, Nevena and Aleksic, Ivana and Kljun, Jakob and Ašanin, Darko and Andrejević, Tina and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Miloš and Glišić, Biljana",
year = "2020",
abstract = "Over the last few decades, invasive fungal infections represent a serious problem for modern-day healthcare. Aspergillus, Candida and Cryptococcus species are the most common pathogens causing life-threatening infections. Therapeutic options for the treatment of fungal infections are presently limited to only four classes of compounds and each of these drug classes has significant therapeutic limitations, including serious toxic-side effects, resistance development and limited routes of administration. In order to overcome resistance of the clinically used antifungal triazole agents, we synthesized zinc(II) and copper(II) complexes with fluconazole (flz), {[ZnCl2(flz)2]·2C2H5OH}n (1) and {[CuCl2(flz)2].5H2O}n (2). These complexes were obtained from the reactions between ZnCl2 or CuCl2·2H2O with this antifungal agent in 1 : 2 molar ratio in ethanol at room temperature. The compounds were characterized by elemental analysis, NMR, IR and UV-Vis spectroscopy and mass spectrometry. The crystal structure of complex 1 was determined by a single-crystal X-ray diffraction analysis. The antimicrobial effect of both complexes and fluconazole was evaluated against different Candida species as well as Gram-positive and Gram-negative bacteria by means of minimal inhibitory concentrations (MICs). The obtained results have shown that, in most cases, the coordination of fluconazole to Zn(II) and Cu(II) ions leads to the enhancement of its antifungal activity. Both complexes showed strong inhibitory activity against C. albicans biofilm formation at concentrations lower than MIC values, as well as strong inhibition of C. albicans filamentation.",
journal = "Electronic Conference on Medicinal Chemistry",
title = "Improvement of antifungal activity and therapeutic profile of fluconazole by its complexation with copper(II) and zinc(II) ions. Complex characterization and antimicrobial activity studies",
doi = "10.3390/ECMC2020-07373"
}

Stevanović, N., Aleksic, I., Kljun, J., Ašanin, D., Andrejević, T., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2020). Improvement of antifungal activity and therapeutic profile of fluconazole by its complexation with copper(II) and zinc(II) ions. Complex characterization and antimicrobial activity studies. in Electronic Conference on Medicinal Chemistry.
https://doi.org/10.3390/ECMC2020-07373

Stevanović N, Aleksic I, Kljun J, Ašanin D, Andrejević T, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Improvement of antifungal activity and therapeutic profile of fluconazole by its complexation with copper(II) and zinc(II) ions. Complex characterization and antimicrobial activity studies. in Electronic Conference on Medicinal Chemistry. 2020;.
doi:10.3390/ECMC2020-07373 .

Stevanović, Nevena, Aleksic, Ivana, Kljun, Jakob, Ašanin, Darko, Andrejević, Tina, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Miloš, Glišić, Biljana, "Improvement of antifungal activity and therapeutic profile of fluconazole by its complexation with copper(II) and zinc(II) ions. Complex characterization and antimicrobial activity studies" in Electronic Conference on Medicinal Chemistry (2020),
https://doi.org/10.3390/ECMC2020-07373 . .

TY  - CONF
AU  - Ašanin, Darko P.
AU  - Andrejević, Tina P.
AU  - Škaro Bogojević, Sanja
AU  - Stevanović, Nevena Lj
AU  - Aleksic, Ivana
AU  - Milivojević, Dušan
AU  - Perdih, Franc
AU  - Turel, Iztok
AU  - Djuran, Miloš I.
AU  - Glišić, Biljana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1632
AB  - New silver(I) complex with thianthrene (tia), [Ag(NO3)(tia)(H2O)]n, was synthesized by the reaction of AgNO3 with an equimolar amount of tia in ethanol/dichloromethane (v/v 1:1) at room temperature, and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. The antimicrobial activity of the synthesized complex was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and Candida spp. This complex showed significant activity toward important human pathogens Gram-positive Staphylococcus aureus and Candida parapsilosis with minimal inhibitory concentrations (MICs) being 3.91 µg/mL. The interaction of [Ag(NO3)(tia)(H2O)]n with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied to evaluate the binding affinity towards these biomolecules for possible insights on the mode of antimicrobial activity. The binding affinity of the investigated complex to BSA is higher than that for DNA, indicating that proteins could be more favorable binding sites for this complex in comparison to the nucleic acids.
PB  - MDPI : Basel,Switzerland
C3  - The 1st International Electronic Conference on Applied Sciences
T1  - Polynuclear Silver(I) Complex with Thianthrene: Structural Characterization, Antimicrobial Activity and Interaction with Biomolecules
IS  - 1
SP  - 4
VL  - 67
DO  - 10.3390/ASEC2020-07534
ER  - 

@conference{
author = "Ašanin, Darko P. and Andrejević, Tina P. and Škaro Bogojević, Sanja and Stevanović, Nevena Lj and Aleksic, Ivana and Milivojević, Dušan and Perdih, Franc and Turel, Iztok and Djuran, Miloš I. and Glišić, Biljana",
year = "2020",
abstract = "New silver(I) complex with thianthrene (tia), [Ag(NO3)(tia)(H2O)]n, was synthesized by the reaction of AgNO3 with an equimolar amount of tia in ethanol/dichloromethane (v/v 1:1) at room temperature, and characterized by NMR, IR and UV-Vis spectroscopy and single-crystal X-ray diffraction analysis. The antimicrobial activity of the synthesized complex was evaluated against the broad panel of Gram-positive and Gram-negative bacteria and Candida spp. This complex showed significant activity toward important human pathogens Gram-positive Staphylococcus aureus and Candida parapsilosis with minimal inhibitory concentrations (MICs) being 3.91 µg/mL. The interaction of [Ag(NO3)(tia)(H2O)]n with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied to evaluate the binding affinity towards these biomolecules for possible insights on the mode of antimicrobial activity. The binding affinity of the investigated complex to BSA is higher than that for DNA, indicating that proteins could be more favorable binding sites for this complex in comparison to the nucleic acids.",
publisher = "MDPI : Basel,Switzerland",
journal = "The 1st International Electronic Conference on Applied Sciences",
title = "Polynuclear Silver(I) Complex with Thianthrene: Structural Characterization, Antimicrobial Activity and Interaction with Biomolecules",
number = "1",
pages = "4",
volume = "67",
doi = "10.3390/ASEC2020-07534"
}

Ašanin, D. P., Andrejević, T. P., Škaro Bogojević, S., Stevanović, N. L., Aleksic, I., Milivojević, D., Perdih, F., Turel, I., Djuran, M. I.,& Glišić, B.. (2020). Polynuclear Silver(I) Complex with Thianthrene: Structural Characterization, Antimicrobial Activity and Interaction with Biomolecules. in The 1st International Electronic Conference on Applied Sciences
MDPI : Basel,Switzerland., 67(1), 4.
https://doi.org/10.3390/ASEC2020-07534

Ašanin DP, Andrejević TP, Škaro Bogojević S, Stevanović NL, Aleksic I, Milivojević D, Perdih F, Turel I, Djuran MI, Glišić B. Polynuclear Silver(I) Complex with Thianthrene: Structural Characterization, Antimicrobial Activity and Interaction with Biomolecules. in The 1st International Electronic Conference on Applied Sciences. 2020;67(1):4.
doi:10.3390/ASEC2020-07534 .

Ašanin, Darko P., Andrejević, Tina P., Škaro Bogojević, Sanja, Stevanović, Nevena Lj, Aleksic, Ivana, Milivojević, Dušan, Perdih, Franc, Turel, Iztok, Djuran, Miloš I., Glišić, Biljana, "Polynuclear Silver(I) Complex with Thianthrene: Structural Characterization, Antimicrobial Activity and Interaction with Biomolecules" in The 1st International Electronic Conference on Applied Sciences, 67, no. 1 (2020):4,
https://doi.org/10.3390/ASEC2020-07534 . .

TY  - CHAP
AU  - Šenerović, Lidija
AU  - Opsenica, Dejan
AU  - Morić, Ivana
AU  - Aleksić, Ivana
AU  - Spasić, M.
AU  - Vasiljević, Branka
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1348
AB  - Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.
PB  - Springer
T2  - Biophysics of Infection
T1  - Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents
EP  - 69
SP  - 37
VL  - 1282
DO  - 10.1007/5584_2019_428
ER  - 

@inbook{
author = "Šenerović, Lidija and Opsenica, Dejan and Morić, Ivana and Aleksić, Ivana and Spasić, M. and Vasiljević, Branka",
year = "2020",
abstract = "Infective diseases have become health threat of a global proportion due to appearance and spread of microorganisms resistant to majority of therapeutics currently used for their treatment. Therefore, there is a constant need for development of new antimicrobial agents, as well as novel therapeutic strategies. Quinolines and quinolones, isolated from plants, animals, and microorganisms, have demonstrated numerous biological activities such as antimicrobial, insecticidal, anti-inflammatory, antiplatelet, and antitumor. For more than two centuries quinoline/quinolone moiety has been used as a scaffold for drug development and even today it represents an inexhaustible inspiration for design and development of novel semi-synthetic or synthetic agents exhibiting broad spectrum of bioactivities. The structural diversity of synthetized compounds provides high and selective activity attained through different mechanisms of action, as well as low toxicity on human cells. This review describes quinoline and quinolone derivatives with antibacterial, antifungal, anti-virulent, antiviral, and anti-parasitic activities with the focus on the last 10 years literature.",
publisher = "Springer",
journal = "Biophysics of Infection",
booktitle = "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents",
pages = "69-37",
volume = "1282",
doi = "10.1007/5584_2019_428"
}

Šenerović, L., Opsenica, D., Morić, I., Aleksić, I., Spasić, M.,& Vasiljević, B.. (2020). Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection
Springer., 1282, 37-69.
https://doi.org/10.1007/5584_2019_428

Šenerović L, Opsenica D, Morić I, Aleksić I, Spasić M, Vasiljević B. Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents. in Biophysics of Infection. 2020;1282:37-69.
doi:10.1007/5584_2019_428 .

Šenerović, Lidija, Opsenica, Dejan, Morić, Ivana, Aleksić, Ivana, Spasić, M., Vasiljević, Branka, "Quinolines and quinolones as antibacterial, antifungal, anti-virulence, antiviral and anti-parasitic agents" in Biophysics of Infection, 1282 (2020):37-69,
https://doi.org/10.1007/5584_2019_428 . .

TY  - THES
AU  - Aleksić, Ivana
PY  - 2019
UR  - https://nardus.mpn.gov.rs/handle/123456789/11827
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7181
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:20876/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1025237170
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/59
AB  - Rezistencija bakterija na antibiotike je rastući problem globalnih razmera tako da su pronalaženje i uvođenje novih terapijskih opcija u borbu protiv bakterijskih infekcija postali prioriteti i fundamentalnih i primenjenih istraživanja.  U potrazi za jedinjenjima prirodnog porekla sa anti-virulentnom aktivnošću izdvojen je nepatogeni soj iz rizosfere biljke dobračice (Glechoma hederacea), Lysinibacillus sp. BV152.1, čiji su metaboliti inhibirali formiranje biofilmova P. aeruginosa PAO1. Hemijske analize su pokazale da aktivnu komponentu etil acetatnog ekstrakta čini smeša ramnolipida. Uporednom analizom anti-biofilm aktivnosti utvrđeno je da su di-ramnolipidi soja Lysinibacillus sp. BV152.1 bolji inhibitori procesa formiranja biofilmova (adhezije i sazrevanja) kod P. aeruginosa PAO1 od komercijalno dostupnih ramnolipida, poreklom iz P. aeruginosa. Amidnom derivatizacijom di-ramnolipida poboljšana je njihova anti-biofilm aktivnost, gde je najaktivniji derivat di-ramnolipida iz Lysinibacillus sp. BV152.1 imao morfolinsku grupu i inhibirao je formiranje biofilmova P. aeruginosa PAO1 za 80% u koncentraciji od 100 μg/ml, a isti derivat u koncentraciji od 50 μg/ml inhibirao je formiranje biofilmova S. marcescens za 88%.  Hinolini, jedinjenja sa širokim spektrom bioloških aktivnosti, a takođe i autoinduceri PQS signalnog puta međubakterijske komunikacije P. aeruginosa predstavljaju dobru osnovu za razvoj anti-virulentnih jedinjenja. Derivati aminohinolina sintetisani u ovoj studiji nisu pokazali značajnu antibakterijsku aktivnost prema Gram negativnim patogenima. Najveću inhibitornu aktivnost na formiranje biofilmova kod P. aeruginosa i S. marcescens imala su jedinjenja koja su sadržala atom hlora ili CF3 grupu na poziciji C(7) i alifatični lanac sa 12 C atoma na poziciji C(4) (jedinjenja 5 i 10), sa BFIC50 koncentracijama od 69 μM odnosno 63 μM. Ova jedinjenja su prvi derivati hinolina za koje je utvrđena sposobnost da inhibiraju biofilmove S. marcescens. Detaljnom analizom odnosa strukture i aktivnosti jedinjenja 5 i 10 je pokazano da dužina alifatičnog lanca i lipofilnost jedinjenja imaju najveći uticaj na formiranja biofilomova kod S. marcescens. Jedinjenje 10 je izdvojeno kao najpotentniji inhibitor proizvodnje piocijanina kod P. aeruginosa hinolinske prirode opisan do sada...
AB  - Bacterial resistance to antibiotics is a growing problem on a global scale, so finding and introducing new therapeutic options to combat bacterial infections has become a priority in both fundamental and applied research.  In a search for structurally new compounds with anti-virulent activity, novel non-pathogenic strain was isolated from the rhizosphere of a Glechoma hederacea plant, named BV152.1, whose metabolites inhibited the formation of P. aeruginosa PAO1 biofilms. Chemical analyzes have shown that the active compound of ethyl acetate extract is a mixture of rhamnolipids. Comparative analysis of their anti-biofilm activity revealed that the di-rhamnolipids from the strain Lysinibacillus sp. BV152.1 are better inhibitors of P. aeruginosa PAO1 biofilm adhesion and maturation, than commercially available rhamnolipids, originated from P. aeruginosa. The amide derivatization of di-rhamnolipids enhanced the anti-biofilm activity of these compounds, where the most active di-rhamnolipid derivative from Lysinibacillus sp. BV152.1 had a morpholine group and inhibited the formation of P. aeruginosa PAO1 biofilms by 80% at a concentration at 100 μg/ml. The same derivative at 50 μg/ml inhibited formation of S. marcescens biofilm by 88%.  Quinolines, compounds with a wide range of biological activities, and autoinducers of the quorum sensing PQS signaling pathway of P. aeruginosa represent a good basis for the development of compounds with anti-virulent activity. The aminoquinoline derivatives synthesized in this study did not show significant antibacterial activity against Gram-negative pathogens, making them suitable chemical structures for the development of anti-virulent agents. The highest inhibitory activity of P. aeruginosa and S. marcescens biofilm formation showed compounds containing a chlorine atom (compound 5) or CF3 group (compound 10) at position C(7) and an aliphatic chain of 12 C atoms at position C(4) with BFIC50 concentrations of 69 μM and 63 μM, respectively. These compounds are the first quinoline derivatives identified to have the ability to inhibit S. marcescens biofilms formation. Detailed analysis of the structure and activity relationships of compounds 5 and 10 showed that the aliphatic chain length and lipophilicity of the compounds had the greatest influence on inhibition of biofilm formation in S. marcescens...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens
T1  - Derivatives of rhamnolipids and 4-aminoquinoline as Pseudomonas aeruginosa and Serratia marcescens virulence inhibitors
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11827
ER  - 

@phdthesis{
author = "Aleksić, Ivana",
year = "2019",
abstract = "Rezistencija bakterija na antibiotike je rastući problem globalnih razmera tako da su pronalaženje i uvođenje novih terapijskih opcija u borbu protiv bakterijskih infekcija postali prioriteti i fundamentalnih i primenjenih istraživanja.  U potrazi za jedinjenjima prirodnog porekla sa anti-virulentnom aktivnošću izdvojen je nepatogeni soj iz rizosfere biljke dobračice (Glechoma hederacea), Lysinibacillus sp. BV152.1, čiji su metaboliti inhibirali formiranje biofilmova P. aeruginosa PAO1. Hemijske analize su pokazale da aktivnu komponentu etil acetatnog ekstrakta čini smeša ramnolipida. Uporednom analizom anti-biofilm aktivnosti utvrđeno je da su di-ramnolipidi soja Lysinibacillus sp. BV152.1 bolji inhibitori procesa formiranja biofilmova (adhezije i sazrevanja) kod P. aeruginosa PAO1 od komercijalno dostupnih ramnolipida, poreklom iz P. aeruginosa. Amidnom derivatizacijom di-ramnolipida poboljšana je njihova anti-biofilm aktivnost, gde je najaktivniji derivat di-ramnolipida iz Lysinibacillus sp. BV152.1 imao morfolinsku grupu i inhibirao je formiranje biofilmova P. aeruginosa PAO1 za 80% u koncentraciji od 100 μg/ml, a isti derivat u koncentraciji od 50 μg/ml inhibirao je formiranje biofilmova S. marcescens za 88%.  Hinolini, jedinjenja sa širokim spektrom bioloških aktivnosti, a takođe i autoinduceri PQS signalnog puta međubakterijske komunikacije P. aeruginosa predstavljaju dobru osnovu za razvoj anti-virulentnih jedinjenja. Derivati aminohinolina sintetisani u ovoj studiji nisu pokazali značajnu antibakterijsku aktivnost prema Gram negativnim patogenima. Najveću inhibitornu aktivnost na formiranje biofilmova kod P. aeruginosa i S. marcescens imala su jedinjenja koja su sadržala atom hlora ili CF3 grupu na poziciji C(7) i alifatični lanac sa 12 C atoma na poziciji C(4) (jedinjenja 5 i 10), sa BFIC50 koncentracijama od 69 μM odnosno 63 μM. Ova jedinjenja su prvi derivati hinolina za koje je utvrđena sposobnost da inhibiraju biofilmove S. marcescens. Detaljnom analizom odnosa strukture i aktivnosti jedinjenja 5 i 10 je pokazano da dužina alifatičnog lanca i lipofilnost jedinjenja imaju najveći uticaj na formiranja biofilomova kod S. marcescens. Jedinjenje 10 je izdvojeno kao najpotentniji inhibitor proizvodnje piocijanina kod P. aeruginosa hinolinske prirode opisan do sada..., Bacterial resistance to antibiotics is a growing problem on a global scale, so finding and introducing new therapeutic options to combat bacterial infections has become a priority in both fundamental and applied research.  In a search for structurally new compounds with anti-virulent activity, novel non-pathogenic strain was isolated from the rhizosphere of a Glechoma hederacea plant, named BV152.1, whose metabolites inhibited the formation of P. aeruginosa PAO1 biofilms. Chemical analyzes have shown that the active compound of ethyl acetate extract is a mixture of rhamnolipids. Comparative analysis of their anti-biofilm activity revealed that the di-rhamnolipids from the strain Lysinibacillus sp. BV152.1 are better inhibitors of P. aeruginosa PAO1 biofilm adhesion and maturation, than commercially available rhamnolipids, originated from P. aeruginosa. The amide derivatization of di-rhamnolipids enhanced the anti-biofilm activity of these compounds, where the most active di-rhamnolipid derivative from Lysinibacillus sp. BV152.1 had a morpholine group and inhibited the formation of P. aeruginosa PAO1 biofilms by 80% at a concentration at 100 μg/ml. The same derivative at 50 μg/ml inhibited formation of S. marcescens biofilm by 88%.  Quinolines, compounds with a wide range of biological activities, and autoinducers of the quorum sensing PQS signaling pathway of P. aeruginosa represent a good basis for the development of compounds with anti-virulent activity. The aminoquinoline derivatives synthesized in this study did not show significant antibacterial activity against Gram-negative pathogens, making them suitable chemical structures for the development of anti-virulent agents. The highest inhibitory activity of P. aeruginosa and S. marcescens biofilm formation showed compounds containing a chlorine atom (compound 5) or CF3 group (compound 10) at position C(7) and an aliphatic chain of 12 C atoms at position C(4) with BFIC50 concentrations of 69 μM and 63 μM, respectively. These compounds are the first quinoline derivatives identified to have the ability to inhibit S. marcescens biofilms formation. Detailed analysis of the structure and activity relationships of compounds 5 and 10 showed that the aliphatic chain length and lipophilicity of the compounds had the greatest influence on inhibition of biofilm formation in S. marcescens...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens, Derivatives of rhamnolipids and 4-aminoquinoline as Pseudomonas aeruginosa and Serratia marcescens virulence inhibitors",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11827"
}

Aleksić, I.. (2019). Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_11827

Aleksić I. Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11827 .

Aleksić, Ivana, "Derivati ramnolipida i 4-aminohinolina kao inhibitori virulencije kod vrsta Pseudomonas aeruginosa i Serratia marcescens" (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11827 .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Jeremić, Jelena
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Segan, Sandra
AU  - Zlatović, Mario
AU  - Opsenica, Dejan M.
AU  - Šenerović, Lidija
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1225
AB  - Pseudomonas aeruginosa is a leading cause of nosocomial infections that are becoming increasingly difficult to treat due to the occurrence of antibiotic resistant strains. Since P. aeruginosa virulence is controlled through quorum sensing, small molecule treatments inhibiting quorum sensing signaling pathways provide a promising therapeutic option. Consequently, we synthesized a series of N-octaneamino-4-aminoquinoline derivatives to optimize this chemotype's antivirulence activity against P. aeruginosa via inhibition of pyocyanin production. The most potent derivative, which possesses a benzofuran substituent, provided effective inhibition of pyocyanin production (IC50 = 12 mu M), biofilm formation (BFIC50 = 50 mu M), and motility. Experimentally, the compound's activity is achieved through competitive inhibition of PqsR, and structure-activity data were rationalized using molecular docking studies.
PB  - Amer Chemical Soc, Washington
T2  - Acs Chemical Biology
T1  - N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa
EP  - 2809
IS  - 12
SP  - 2800
VL  - 14
DO  - 10.1021/acschembio.9b00682
ER  - 

@article{
author = "Aleksić, Ivana and Jeremić, Jelena and Milivojević, Dušan and Ilić-Tomić, Tatjana and Segan, Sandra and Zlatović, Mario and Opsenica, Dejan M. and Šenerović, Lidija",
year = "2019",
abstract = "Pseudomonas aeruginosa is a leading cause of nosocomial infections that are becoming increasingly difficult to treat due to the occurrence of antibiotic resistant strains. Since P. aeruginosa virulence is controlled through quorum sensing, small molecule treatments inhibiting quorum sensing signaling pathways provide a promising therapeutic option. Consequently, we synthesized a series of N-octaneamino-4-aminoquinoline derivatives to optimize this chemotype's antivirulence activity against P. aeruginosa via inhibition of pyocyanin production. The most potent derivative, which possesses a benzofuran substituent, provided effective inhibition of pyocyanin production (IC50 = 12 mu M), biofilm formation (BFIC50 = 50 mu M), and motility. Experimentally, the compound's activity is achieved through competitive inhibition of PqsR, and structure-activity data were rationalized using molecular docking studies.",
publisher = "Amer Chemical Soc, Washington",
journal = "Acs Chemical Biology",
title = "N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa",
pages = "2809-2800",
number = "12",
volume = "14",
doi = "10.1021/acschembio.9b00682"
}

Aleksić, I., Jeremić, J., Milivojević, D., Ilić-Tomić, T., Segan, S., Zlatović, M., Opsenica, D. M.,& Šenerović, L.. (2019). N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa. in Acs Chemical Biology
Amer Chemical Soc, Washington., 14(12), 2800-2809.
https://doi.org/10.1021/acschembio.9b00682

Aleksić I, Jeremić J, Milivojević D, Ilić-Tomić T, Segan S, Zlatović M, Opsenica DM, Šenerović L. N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa. in Acs Chemical Biology. 2019;14(12):2800-2809.
doi:10.1021/acschembio.9b00682 .

Aleksić, Ivana, Jeremić, Jelena, Milivojević, Dušan, Ilić-Tomić, Tatjana, Segan, Sandra, Zlatović, Mario, Opsenica, Dejan M., Šenerović, Lidija, "N-Benzyl Derivatives of Long-Chained 4-Amino-7-chloro-quionolines as Inhibitors of Pyocyanin Production in Pseudomonas aeruginosa" in Acs Chemical Biology, 14, no. 12 (2019):2800-2809,
https://doi.org/10.1021/acschembio.9b00682 . .

TY  - DATA
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1768
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005
VL  - 222
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1768
ER  - 

@misc{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005",
volume = "222",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1768"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222.
https://hdl.handle.net/21.15107/rcub_imagine_1768

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005. in Journal of Ethnopharmacology. 2018;222.
https://hdl.handle.net/21.15107/rcub_imagine_1768 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Supplementary data for the article: Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D., & Šenerović, L. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. Journal of Ethnopharmacology, 222, 148–158. https://doi.org/10.1016/j.jep.2018.05.005" in Journal of Ethnopharmacology, 222 (2018),
https://hdl.handle.net/21.15107/rcub_imagine_1768 .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1762
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
EP  - 158
SP  - 148
VL  - 222
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
pages = "158-148",
volume = "222",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .

TY  - JOUR
AU  - Aleksić, Ivana
AU  - Ristivojević, Petar
AU  - Pavić, Aleksandar
AU  - Radojević, Ivana
AU  - Comić, Ljiljana R.
AU  - Vasiljević, Branka
AU  - Opsenica, Dejan
AU  - Milojkovic-Opsenica, Dusanka
AU  - Šenerović, Lidija
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1111
AB  - Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts
EP  - 158
SP  - 148
VL  - 222
DO  - 10.1016/j.jep.2018.05.005
ER  - 

@article{
author = "Aleksić, Ivana and Ristivojević, Petar and Pavić, Aleksandar and Radojević, Ivana and Comić, Ljiljana R. and Vasiljević, Branka and Opsenica, Dejan and Milojkovic-Opsenica, Dusanka and Šenerović, Lidija",
year = "2018",
abstract = "Ethnopharmacological relevance: Trapa natans L. (water chestnut or water caltrop) is a widespread aquatic plant, which has been cultivated for food and traditional medicine since ancient times. Pharmacological studies showed that water chestnut exhibits the wide range of biological activities, such as antimicrobial, antioxidative, analgesic, anti-inflammatory, as well as antiulcer. Aim of the study: Evaluation of anti-virulence potential and toxicity of T. natans methanol (TnM), acetone (TnA) and ethyl acetate (TnEA) leaf extracts. Materials and methods: The anti-quorum sensing activity of Tn extracts was addressed by measuring their effects on biofilm formation, swarming motility and pyocyanin and elastase production in Pseudomonas aeruginosa. Specific P. aeruginosa biosensors were used to identify which of the signaling pathways were affected. The lethal and developmental toxicity of extracts were addressed in vivo using the zebrafish (Danio rerio) model system. The phenolic composition of T. natans leafs extracts was analyzed by a linear ion trap-OrbiTrap hybrid mass spectrometer (LTQ OrbiTrapMS) and UHPLC system configured with a diode array detector (DAD) hyphenated with the triple quadrupole mass spectrometer. Results: Subinhibitory concentrations of Tn leaf extracts (0.2 MIC) inhibited pyocyanin and elastase production up to 50% and 60%, respectively, and reduced swarming zones, comparing to non-treated P. aeruginosa. TnA inhibited biofilm formation by 15%, TnM showed a stimulatory effect on biofilm formation up to 20%, while TnEA showed no effect. The bioactive concentrations of TnM and TnA were not toxic in the zebrafish model system. Twenty-two phenolic compounds were tentatively identified in TnM, where thirteen of them were identified in T. natans for the first time. Tn extracts, as well as their major components, ellagic and ferulic acids, demonstrated the ability to interfere with P. aeruginosa Las and PQS signaling pathways. Conclusions: This study demonstrates anti-virulence potential of Tn leaf extracts against medically important pathogen P. aeruginosa and confirms the ethnopharmacological application of this plant against microbial infections.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts",
pages = "158-148",
volume = "222",
doi = "10.1016/j.jep.2018.05.005"
}

Aleksić, I., Ristivojević, P., Pavić, A., Radojević, I., Comić, L. R., Vasiljević, B., Opsenica, D., Milojkovic-Opsenica, D.,& Šenerović, L.. (2018). Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 222, 148-158.
https://doi.org/10.1016/j.jep.2018.05.005

Aleksić I, Ristivojević P, Pavić A, Radojević I, Comić LR, Vasiljević B, Opsenica D, Milojkovic-Opsenica D, Šenerović L. Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts. in Journal of Ethnopharmacology. 2018;222:148-158.
doi:10.1016/j.jep.2018.05.005 .

Aleksić, Ivana, Ristivojević, Petar, Pavić, Aleksandar, Radojević, Ivana, Comić, Ljiljana R., Vasiljević, Branka, Opsenica, Dejan, Milojkovic-Opsenica, Dusanka, Šenerović, Lidija, "Anti-quorum sensing activity, toxicity in zebrafish (Danio rerio) embryos and phytochemical characterization of Trapa natans leaf extracts" in Journal of Ethnopharmacology, 222 (2018):148-158,
https://doi.org/10.1016/j.jep.2018.05.005 . .