TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Cosović, Vladan
AU  - Atanase, Leonard Ionut
AU  - Freitas, Filomena
AU  - Matos, Mariana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1714
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
IS  - 3
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Cosović, Vladan and Atanase, Leonard Ionut and Freitas, Filomena and Matos, Mariana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
number = "3",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Cosović, V., Atanase, L. I., Freitas, F., Matos, M.,& Nikodinović-Runić, J.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences, 24(3), 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Cosović V, Atanase LI, Freitas F, Matos M, Nikodinović-Runić J. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24(3):1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Cosović, Vladan, Atanase, Leonard Ionut, Freitas, Filomena, Matos, Mariana, Nikodinović-Runić, Jasmina, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24, no. 3 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - DATA
AU  - Solarz, Daria
AU  - Witko, Tomasz
AU  - Karcz, Robert
AU  - Malagurski, Ivana
AU  - Ponjavić, Marijana
AU  - Levic, Steva
AU  - Nešić, Aleksandra
AU  - Guzik, Maciej
AU  - Savić, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra03021k
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2060
AB  - Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.
T2  - RSC Advances
T1  - Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2060
ER  - 

@misc{
author = "Solarz, Daria and Witko, Tomasz and Karcz, Robert and Malagurski, Ivana and Ponjavić, Marijana and Levic, Steva and Nešić, Aleksandra and Guzik, Maciej and Savić, Sanja and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.",
journal = "RSC Advances",
title = "Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2060"
}

Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K. in RSC Advances.
https://hdl.handle.net/21.15107/rcub_imagine_2060

Solarz D, Witko T, Karcz R, Malagurski I, Ponjavić M, Levic S, Nešić A, Guzik M, Savić S, Nikodinović-Runić J. Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K. in RSC Advances. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2060 .

Solarz, Daria, Witko, Tomasz, Karcz, Robert, Malagurski, Ivana, Ponjavić, Marijana, Levic, Steva, Nešić, Aleksandra, Guzik, Maciej, Savić, Sanja, Nikodinović-Runić, Jasmina, "Supplementary data for article: Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128. https://doi.org/10.1039/D3RA03021K" in RSC Advances (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2060 .

TY  - JOUR
AU  - Solarz, Daria
AU  - Witko, Tomasz
AU  - Karcz, Robert
AU  - Malagurski, Ivana
AU  - Ponjavić, Marijana
AU  - Levic, Steva
AU  - Nešić, Aleksandra
AU  - Guzik, Maciej
AU  - Savić, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://pubs.rsc.org/en/content/articlelanding/2023/ra/d3ra03021k
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2059
AB  - Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.
T2  - RSC Advances
T1  - Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications
EP  - 24128
IS  - 34
SP  - 24112
VL  - 13
DO  - 10.1039/D3RA03021K
ER  - 

@article{
author = "Solarz, Daria and Witko, Tomasz and Karcz, Robert and Malagurski, Ivana and Ponjavić, Marijana and Levic, Steva and Nešić, Aleksandra and Guzik, Maciej and Savić, Sanja and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyoctanoate, as a biocompatible and biodegradable biopolymer, represents an ideal candidate for biomedical applications. However, physical properties make it unsuitable for electrospinning, currently the most widely used technique for fabrication of fibrous scaffolds. To overcome this, it was blended with polylactic acid and polymer blend fibrous biomaterials were produced by electrospinning. The obtained PLA/PHO fibers were cylindrical, smaller in size, more hydrophilic and had a higher degree of biopolymer crystallinity and more favorable mechanical properties in comparison to the pure PLA sample. Cytotoxicity evaluation with human lung fibroblasts (MRC5 cells) combined with confocal microscopy were used to visualize mouse embryonic fibroblasts (MEF 3T3 cell line) migration and distribution showed that PLA/PHO samples support exceptional cell adhesion and viability, indicating excellent biocompatibility. The obtained results suggest that PLA/PHO fibrous biomaterials can be potentially used as biocompatible, biomimetic scaffolds for tissue engineering applications.",
journal = "RSC Advances",
title = "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications",
pages = "24128-24112",
number = "34",
volume = "13",
doi = "10.1039/D3RA03021K"
}

Solarz, D., Witko, T., Karcz, R., Malagurski, I., Ponjavić, M., Levic, S., Nešić, A., Guzik, M., Savić, S.,& Nikodinović-Runić, J.. (2023). Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances, 13(34), 24112-24128.
https://doi.org/10.1039/D3RA03021K

Solarz D, Witko T, Karcz R, Malagurski I, Ponjavić M, Levic S, Nešić A, Guzik M, Savić S, Nikodinović-Runić J. Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications. in RSC Advances. 2023;13(34):24112-24128.
doi:10.1039/D3RA03021K .

Solarz, Daria, Witko, Tomasz, Karcz, Robert, Malagurski, Ivana, Ponjavić, Marijana, Levic, Steva, Nešić, Aleksandra, Guzik, Maciej, Savić, Sanja, Nikodinović-Runić, Jasmina, "Biological and physiochemical studies of electrospun polylactid/polyhydroxyoctanoate PLA/ P(3HO) scaffolds for tissue engineering applications" in RSC Advances, 13, no. 34 (2023):24112-24128,
https://doi.org/10.1039/D3RA03021K . .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Jeremić, Sanja
AU  - Malagurski, Ivana
AU  - Babu P., Ramesh
AU  - Rajasekaran, Divya
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2236
AB  - The rapid increase in global plastics production is
also causing an accelerated environmental
pollution. Recently, biotechnological solutions and
enzymatic recycling of poly(ethylene terephthalate)
(PET) waste stream have been put forward and
commercialized1. Increasing recycling and
upcycling rates is the most effective model
approach to plastic circularity. However, mixed
plastic waste is still quite a challenge for both
recycling and upcycling technologies. This study is
focused on the eco-conversion of plastic waste
containing poly(ethylene terephthalate), PET, into
biopolymer, bacterial nanocellulose. Polymer mix
contained selection of commercial biodegradable
plastics (poly(lactic acid), PLA, poly(ε-caprolactone),
PCL, poly(hyoxyl butyrate), PHB) and PET. This
mixture was hydrolysed under aqueous conditions
and hydrolysate was used as carbon source for
Komagataeibacter medellinensis ID13488 and
bacterial nanocellulose (BNC) production. HPLC
analysis confirmed the presence of monomers and
dimers of polymer mix components indicating
existence of potential substrates for BNC
production. BNC production by K. medellinensis
was investigated and optimized in ter of the
amount of carbon source and growth conditions.
Under the most efficient rate in ter of yield, BNC
production was scaled up and the obtained
biopolymer was characterized. The structure of
produced BNC was confirmed by FTIR analysis,
thermal properties by DSC/TG analysis, and the
morphology of material by optical microscopy and
SEM analysis. This research demonstrates how to
put the mixed plastic waste stream into a circular
loop through the biotechnological conversion into
valuable biopolymer.
C3  - 10th Conference of Mikrobiokosmos
T1  - Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2236
ER  - 

@conference{
author = "Ponjavić, Marijana and Jeremić, Sanja and Malagurski, Ivana and Babu P., Ramesh and Rajasekaran, Divya and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The rapid increase in global plastics production is
also causing an accelerated environmental
pollution. Recently, biotechnological solutions and
enzymatic recycling of poly(ethylene terephthalate)
(PET) waste stream have been put forward and
commercialized1. Increasing recycling and
upcycling rates is the most effective model
approach to plastic circularity. However, mixed
plastic waste is still quite a challenge for both
recycling and upcycling technologies. This study is
focused on the eco-conversion of plastic waste
containing poly(ethylene terephthalate), PET, into
biopolymer, bacterial nanocellulose. Polymer mix
contained selection of commercial biodegradable
plastics (poly(lactic acid), PLA, poly(ε-caprolactone),
PCL, poly(hyoxyl butyrate), PHB) and PET. This
mixture was hydrolysed under aqueous conditions
and hydrolysate was used as carbon source for
Komagataeibacter medellinensis ID13488 and
bacterial nanocellulose (BNC) production. HPLC
analysis confirmed the presence of monomers and
dimers of polymer mix components indicating
existence of potential substrates for BNC
production. BNC production by K. medellinensis
was investigated and optimized in ter of the
amount of carbon source and growth conditions.
Under the most efficient rate in ter of yield, BNC
production was scaled up and the obtained
biopolymer was characterized. The structure of
produced BNC was confirmed by FTIR analysis,
thermal properties by DSC/TG analysis, and the
morphology of material by optical microscopy and
SEM analysis. This research demonstrates how to
put the mixed plastic waste stream into a circular
loop through the biotechnological conversion into
valuable biopolymer.",
journal = "10th Conference of Mikrobiokosmos",
title = "Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2236"
}

Ponjavić, M., Jeremić, S., Malagurski, I., Babu P., R., Rajasekaran, D., Topakas, E.,& Nikodinović-Runić, J.. (2023). Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose. in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2236

Ponjavić M, Jeremić S, Malagurski I, Babu P. R, Rajasekaran D, Topakas E, Nikodinović-Runić J. Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose. in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2236 .

Ponjavić, Marijana, Jeremić, Sanja, Malagurski, Ivana, Babu P., Ramesh, Rajasekaran, Divya, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2236 .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Salevic-Jelic, Ana
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2106
AB  - New film materials based on bacterial biomolecules polyhydroxyalkanoate (poly(3-
hydroxybutyrate-co-3-hydroxyvalerate) PHBV) and prodigiosin (PG) were produced by
solvent casting as a potential food packaging material. Film precursors were obtained in a
sustainable manner via microbial fermentation using waste stream-based substrates (cooking oil
and second-grade canned meat, after the expiry date). The incorporation of PG into the PHBV has
influenced the morphology and functionality of the obtained materials. PG acted as a nucleating
agent, affecting in turn PHBV/PG film surface morphology. The films were intensively colored,
transparent and blocked UV-light. An increase in PG content decreased film transparency but it
did not affect UV-blocking ability. Migration experiments have shown that films possess the
potential to release PG into lipophilic food simulant media where it has exhibited antioxidative
action. The obtained results suggest that PHBV/PG films can be potentially used as sustainable
and active food packaging materials.
PB  - Kragujevac : Institute for Information Technologies
C3  - 2nd International Conference on Chemo and Bioinformatics
T1  - UV-blocking sustainable food packaging based on polyhydroxyalkanoate and bacterial pigment prodigiosin
EP  - 1354
SP  - 1351
DO  - 10.46793/ICCBI23.351P
ER  - 

@conference{
author = "Ponjavić, Marijana and Malagurski, Ivana and Salevic-Jelic, Ana and Lazić, Jelena and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "New film materials based on bacterial biomolecules polyhydroxyalkanoate (poly(3-
hydroxybutyrate-co-3-hydroxyvalerate) PHBV) and prodigiosin (PG) were produced by
solvent casting as a potential food packaging material. Film precursors were obtained in a
sustainable manner via microbial fermentation using waste stream-based substrates (cooking oil
and second-grade canned meat, after the expiry date). The incorporation of PG into the PHBV has
influenced the morphology and functionality of the obtained materials. PG acted as a nucleating
agent, affecting in turn PHBV/PG film surface morphology. The films were intensively colored,
transparent and blocked UV-light. An increase in PG content decreased film transparency but it
did not affect UV-blocking ability. Migration experiments have shown that films possess the
potential to release PG into lipophilic food simulant media where it has exhibited antioxidative
action. The obtained results suggest that PHBV/PG films can be potentially used as sustainable
and active food packaging materials.",
publisher = "Kragujevac : Institute for Information Technologies",
journal = "2nd International Conference on Chemo and Bioinformatics",
title = "UV-blocking sustainable food packaging based on polyhydroxyalkanoate and bacterial pigment prodigiosin",
pages = "1354-1351",
doi = "10.46793/ICCBI23.351P"
}

Ponjavić, M., Malagurski, I., Salevic-Jelic, A., Lazić, J.,& Nikodinović-Runić, J.. (2023). UV-blocking sustainable food packaging based on polyhydroxyalkanoate and bacterial pigment prodigiosin. in 2nd International Conference on Chemo and Bioinformatics
Kragujevac : Institute for Information Technologies., 1351-1354.
https://doi.org/10.46793/ICCBI23.351P

Ponjavić M, Malagurski I, Salevic-Jelic A, Lazić J, Nikodinović-Runić J. UV-blocking sustainable food packaging based on polyhydroxyalkanoate and bacterial pigment prodigiosin. in 2nd International Conference on Chemo and Bioinformatics. 2023;:1351-1354.
doi:10.46793/ICCBI23.351P .

Ponjavić, Marijana, Malagurski, Ivana, Salevic-Jelic, Ana, Lazić, Jelena, Nikodinović-Runić, Jasmina, "UV-blocking sustainable food packaging based on polyhydroxyalkanoate and bacterial pigment prodigiosin" in 2nd International Conference on Chemo and Bioinformatics (2023):1351-1354,
https://doi.org/10.46793/ICCBI23.351P . .

TY  - JOUR
AU  - Pavić, Aleksandar
AU  - Stojanović, Zoran
AU  - Pekmezović, Marina
AU  - Veljović, Đorđe
AU  - O'Connor, Kevin
AU  - Malagurski, Ivana
AU  - Nikodinović-Runić, Jasmina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1554
AB  - Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7-8 mu m in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 x MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.
PB  - MDPI, Basel
T2  - Pharmaceutics
T1  - Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model
IS  - 4
VL  - 14
DO  - 10.3390/pharmaceutics14040696
ER  - 

@article{
author = "Pavić, Aleksandar and Stojanović, Zoran and Pekmezović, Marina and Veljović, Đorđe and O'Connor, Kevin and Malagurski, Ivana and Nikodinović-Runić, Jasmina",
year = "2022",
abstract = "Immobilizing antifungal polyenes such as nystatin (Nys) and amphotericin B (AmB) into biodegradable formulations is advantageous compared to free drug administration providing sustained release, reduced dosing due to localized targeting and overall reduced systemic drug toxicity. In this study, we encapsulated Nys and AmB in medium chain length polyhydroxyalkanoates (mcl-PHA) microspheres (7-8 mu m in diameter). The obtained formulations have been validated for antifungal activity in vitro against a panel of pathogenic fungi including species of Candida, Aspergillus, Microsporum and Trichophyton genera and toxicity and efficacy in vivo using the zebrafish model of disseminated candidiasis. While free polyenes, especially AmB, were highly toxic to zebrafish embryos at the effective (MIC) doses, after their loading into mcl-PHA microspheres, inner organ toxicity and teratogenicity associated with both drugs were not observed, even at 100 x MIC doses. The obtained mcl-PHA/polyene formulations have successfully eradicated C. albicans infection and showed an improved therapeutic profile in zebrafish by enhancing infected embryos survival. This approach is contributing to the antifungal arsenal as polyenes, although the first broad-spectrum antifungals on the market are still the gold standard for treatment of fungal infections.",
publisher = "MDPI, Basel",
journal = "Pharmaceutics",
title = "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model",
number = "4",
volume = "14",
doi = "10.3390/pharmaceutics14040696"
}

Pavić, A., Stojanović, Z., Pekmezović, M., Veljović, Đ., O'Connor, K., Malagurski, I.,& Nikodinović-Runić, J.. (2022). Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics
MDPI, Basel., 14(4).
https://doi.org/10.3390/pharmaceutics14040696

Pavić A, Stojanović Z, Pekmezović M, Veljović Đ, O'Connor K, Malagurski I, Nikodinović-Runić J. Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model. in Pharmaceutics. 2022;14(4).
doi:10.3390/pharmaceutics14040696 .

Pavić, Aleksandar, Stojanović, Zoran, Pekmezović, Marina, Veljović, Đorđe, O'Connor, Kevin, Malagurski, Ivana, Nikodinović-Runić, Jasmina, "Polyenes in Medium Chain Length Polyhydroxyalkanoate (mcl-PHA) Biopolymer Microspheres with Reduced Toxicity and Improved Therapeutic Effect against Candida Infection in Zebrafish Model" in Pharmaceutics, 14, no. 4 (2022),
https://doi.org/10.3390/pharmaceutics14040696 . .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Krusić, Melina Kalagasidis
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1440
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Krusić, Melina Kalagasidis and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Krusić, M. K., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel
MDPI, Basel., 10(6).
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Krusić MK, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel. 2021;10(6).
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Krusić, Melina Kalagasidis, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics-Basel, 10, no. 6 (2021),
https://doi.org/10.3390/antibiotics10060737 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani K.
AU  - Neels, Antonia
AU  - Anđelković, Boban
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh
AU  - O'Connor, Kevin 
AU  - Witko, Tomasz
AU  - Solarz, Daria
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1481
AB  - New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application
VL  - 285
DO  - 10.1016/j.matlet.2020.129100
ER  - 

@article{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani K. and Neels, Antonia and Anđelković, Boban and Senthamaraikannan, Ramsankar and Babu, Ramesh and O'Connor, Kevin  and Witko, Tomasz and Solarz, Daria and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application",
volume = "285",
doi = "10.1016/j.matlet.2020.129100"
}

Malagurski, I., Frison, R., Maurya, A. K., Neels, A., Anđelković, B., Senthamaraikannan, R., Babu, R., O'Connor, K., Witko, T., Solarz, D.,& Nikodinović-Runić, J.. (2021). Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters
Elsevier, Amsterdam., 285.
https://doi.org/10.1016/j.matlet.2020.129100

Malagurski I, Frison R, Maurya AK, Neels A, Anđelković B, Senthamaraikannan R, Babu R, O'Connor K, Witko T, Solarz D, Nikodinović-Runić J. Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters. 2021;285.
doi:10.1016/j.matlet.2020.129100 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani K., Neels, Antonia, Anđelković, Boban, Senthamaraikannan, Ramsankar, Babu, Ramesh, O'Connor, Kevin , Witko, Tomasz, Solarz, Daria, Nikodinović-Runić, Jasmina, "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application" in Materials Letters, 285 (2021),
https://doi.org/10.1016/j.matlet.2020.129100 . .

TY  - CONF
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani
AU  - Nikodinović-Runić, Jasmina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Neels, Antonia
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1216
AB  - Medium-chain polyhydroxyalkanoates (mcl-PHA) are flexible, elastomeric polymers produced by wide range of
bacteria as intercellular storage of carbon and energy. They represent attractive components in biomaterial design
because they are biocompatible, biodegradable and can be obtained using variety of carbon sources including
waste streams[1]. However, being semi-crystalline, all mcl-PHAs are characterized by low melting temperature and
poor tensile strength which can interfere with processing methods and wider biomedical application. Simple way
to improve mcl-PHAs properties is to incorporate a nanophase within biopolymer to obtain nanocomposites.
Nano-sized constituents interact with biopolymer more intimately affecting in turn the obtained nanocomposite
properties as well as functionality. Among inorganic nanofillers, TiO2 nanostructures with high aspect ratio (e.g.
nanofibers) have unique properties that support osteogenic phenotype which makes them suitable for bone tissue
engineering [2].
PB  - Int Union Crystallography, Chester
C3  - Acta Crystallographica A-Foundation and Advances
T1  - Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd
VL  - 75
DO  - 10.1107/S2053273319089794
ER  - 

@conference{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani and Nikodinović-Runić, Jasmina and Babu, Ramesh and O'Connor, Kevin and Neels, Antonia",
year = "2019",
abstract = "Medium-chain polyhydroxyalkanoates (mcl-PHA) are flexible, elastomeric polymers produced by wide range of
bacteria as intercellular storage of carbon and energy. They represent attractive components in biomaterial design
because they are biocompatible, biodegradable and can be obtained using variety of carbon sources including
waste streams[1]. However, being semi-crystalline, all mcl-PHAs are characterized by low melting temperature and
poor tensile strength which can interfere with processing methods and wider biomedical application. Simple way
to improve mcl-PHAs properties is to incorporate a nanophase within biopolymer to obtain nanocomposites.
Nano-sized constituents interact with biopolymer more intimately affecting in turn the obtained nanocomposite
properties as well as functionality. Among inorganic nanofillers, TiO2 nanostructures with high aspect ratio (e.g.
nanofibers) have unique properties that support osteogenic phenotype which makes them suitable for bone tissue
engineering [2].",
publisher = "Int Union Crystallography, Chester",
journal = "Acta Crystallographica A-Foundation and Advances",
title = "Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd",
volume = "75",
doi = "10.1107/S2053273319089794"
}

Malagurski, I., Frison, R., Maurya, A., Nikodinović-Runić, J., Babu, R., O'Connor, K.,& Neels, A.. (2019). Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd. in Acta Crystallographica A-Foundation and Advances
Int Union Crystallography, Chester., 75.
https://doi.org/10.1107/S2053273319089794

Malagurski I, Frison R, Maurya A, Nikodinović-Runić J, Babu R, O'Connor K, Neels A. Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd. in Acta Crystallographica A-Foundation and Advances. 2019;75.
doi:10.1107/S2053273319089794 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani, Nikodinović-Runić, Jasmina, Babu, Ramesh, O'Connor, Kevin, Neels, Antonia, "Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd" in Acta Crystallographica A-Foundation and Advances, 75 (2019),
https://doi.org/10.1107/S2053273319089794 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nesić, Aleksandra
AU  - Cabrera-Barjas, Gustavo
AU  - Kalusević, Ana
AU  - Nedović, Viktor
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1237
AB  - Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films
VL  - 255
DO  - 10.1016/j.matlet.2019.126572
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Nesić, Aleksandra and Cabrera-Barjas, Gustavo and Kalusević, Ana and Nedović, Viktor and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "Agar-based composites with different Zn-carbonate mineral phase content were prepared by in situ mineralization and the solvent casting method. The mineral phase within the composite films was identified as hydrozincite, Zn-5(CO3)(2)(OH)(6). The presence of the mineral phase improved, both mechanical and water vapor permeability properties of the obtained composite films, in a concentration-dependent manner. The release of zinc ions from composite films is in accepted levels (up to 2.5%), and sufficient to provide complete inhibition growth of S. Aureus. The results of this study suggest that agar/Zn-carbonate composites could be potentially used as affordable, eco-friendly and functional materials with tunable properties for food packaging, agriculture or biomedical application. In situ procedure offers possibilities for tailoring the physical-chemical properties of composite films, by varying the Zn-mineral phase load.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films",
volume = "255",
doi = "10.1016/j.matlet.2019.126572"
}

Radovanović, N., Malagurski, I., Lević, S., Nesić, A., Cabrera-Barjas, G., Kalusević, A., Nedović, V., Pavlović, V.,& Dimitrijević-Branković, S.. (2019). Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters
Elsevier, Amsterdam., 255.
https://doi.org/10.1016/j.matlet.2019.126572

Radovanović N, Malagurski I, Lević S, Nesić A, Cabrera-Barjas G, Kalusević A, Nedović V, Pavlović V, Dimitrijević-Branković S. Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films. in Materials Letters. 2019;255.
doi:10.1016/j.matlet.2019.126572 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Nesić, Aleksandra, Cabrera-Barjas, Gustavo, Kalusević, Ana, Nedović, Viktor, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Influence of different concentrations of Zn-carbonate phase on physical-chemical properties of antimicrobial agar composite films" in Materials Letters, 255 (2019),
https://doi.org/10.1016/j.matlet.2019.126572 . .

TY  - JOUR
AU  - Radovanović, Neda
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Gordić, Milan
AU  - Petrović, Jelena
AU  - Pavlović, Vladimir
AU  - Mitrić, Miodrag
AU  - Nesić, Aleksandra
AU  - Dimitrijević-Branković, Suzana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1200
AB  - New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - European Polymer Journal
T1  - Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase
EP  - 358
SP  - 352
VL  - 119
DO  - 10.1016/j.eurpolymj.2019.08.004
ER  - 

@article{
author = "Radovanović, Neda and Malagurski, Ivana and Lević, Steva and Gordić, Milan and Petrović, Jelena and Pavlović, Vladimir and Mitrić, Miodrag and Nesić, Aleksandra and Dimitrijević-Branković, Suzana",
year = "2019",
abstract = "New agar-based composite films with increasing Cu-carbonate and Cu-phosphate mineral phase content were prepared by in situ mineralization and solvent casting method. SEM and optical analysis revealed that Cu-carbonate phase had better compatibility with agar matrix than Cu-phosphate phase. Incorporation of both mineral phases improved mechanical and water vapor barrier properties of the obtained mineralized films, in concentration dependent manner. When 5 mM of carbonate precursor was incorporated into agar matrix, mechanical resistance was enchanced for 44% and water vapor barrier property for 40%. The release of Cu (II) was higher in acidic conditions for both mineralized composites and remained in the range of specific release limits for this metal. In addition, both mineralized composite films exhibited distinctive antimicrobial activity against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Overall, the Cu-carbonate and Cu-phosphate mineralized agar films showed potential to be used for food packaging materials, agriculture or medical purposes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "European Polymer Journal",
title = "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase",
pages = "358-352",
volume = "119",
doi = "10.1016/j.eurpolymj.2019.08.004"
}

Radovanović, N., Malagurski, I., Lević, S., Gordić, M., Petrović, J., Pavlović, V., Mitrić, M., Nesić, A.,& Dimitrijević-Branković, S.. (2019). Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal
Pergamon-Elsevier Science Ltd, Oxford., 119, 352-358.
https://doi.org/10.1016/j.eurpolymj.2019.08.004

Radovanović N, Malagurski I, Lević S, Gordić M, Petrović J, Pavlović V, Mitrić M, Nesić A, Dimitrijević-Branković S. Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase. in European Polymer Journal. 2019;119:352-358.
doi:10.1016/j.eurpolymj.2019.08.004 .

Radovanović, Neda, Malagurski, Ivana, Lević, Steva, Gordić, Milan, Petrović, Jelena, Pavlović, Vladimir, Mitrić, Miodrag, Nesić, Aleksandra, Dimitrijević-Branković, Suzana, "Tailoring the physico-chemical and antimicrobial properties of agar-based films by in situ formation of Cu-mineral phase" in European Polymer Journal, 119 (2019):352-358,
https://doi.org/10.1016/j.eurpolymj.2019.08.004 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1169
AB  - Two bimetallic (Zn/Cu) alginate based nanocomposites, impregnated with carbonate or phosphate mineral phase, were prepared by a facile procedure. Mineralized samples exhibited different morphologies and properties when compared to the non-mineralized sample. Antimicrobial testing against Escherichia coil, Staphylococcus aureus and Candida albicans showed that mineralized samples are more efficient than non-mineralized in elimination of microorganisms. The results of this study suggest that bimetallic mineralized alginates could be potentially used as affordable, easy to produce antimicrobial materials.
PB  - Elsevier Science Bv, Amsterdam
T2  - Materials Letters
T1  - Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application
EP  - 36
SP  - 32
VL  - 212
DO  - 10.1016/j.matlet.2017.10.046
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Mitrić, Miodrag and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2018",
abstract = "Two bimetallic (Zn/Cu) alginate based nanocomposites, impregnated with carbonate or phosphate mineral phase, were prepared by a facile procedure. Mineralized samples exhibited different morphologies and properties when compared to the non-mineralized sample. Antimicrobial testing against Escherichia coil, Staphylococcus aureus and Candida albicans showed that mineralized samples are more efficient than non-mineralized in elimination of microorganisms. The results of this study suggest that bimetallic mineralized alginates could be potentially used as affordable, easy to produce antimicrobial materials.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Materials Letters",
title = "Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application",
pages = "36-32",
volume = "212",
doi = "10.1016/j.matlet.2017.10.046"
}

Malagurski, I., Lević, S., Mitrić, M., Pavlović, V.,& Dimitrijević-Branković, S.. (2018). Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application. in Materials Letters
Elsevier Science Bv, Amsterdam., 212, 32-36.
https://doi.org/10.1016/j.matlet.2017.10.046

Malagurski I, Lević S, Mitrić M, Pavlović V, Dimitrijević-Branković S. Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application. in Materials Letters. 2018;212:32-36.
doi:10.1016/j.matlet.2017.10.046 .

Malagurski, Ivana, Lević, Steva, Mitrić, Miodrag, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Bimetallic alginate nanocomposites: New antimicrobial biomaterials for biomedical application" in Materials Letters, 212 (2018):32-36,
https://doi.org/10.1016/j.matlet.2017.10.046 . .

TY  - THES
AU  - Malagurski, Ivana
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5663
UR  - https://nardus.mpn.gov.rs/handle/123456789/9303
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:17314/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49912079
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/46
AB  - U ovoj tezi je ispitana mogućnost sinteze novih, poboljšanih biomaterijala na bazi alginata, agara i cinka, primenom biomimetičkog principa dizajna – biomineralizacije. Cilj istraživanja je bio dizajn i karakterizacija novih nanokompozitnih biomaterijala, sa potencijalnom primenom u biomedicini i pakovanju hrane...
AB  - The aim of this study was to test the hypothesis whether biopolymer-based biomineralization, with essential metal salts, Zn-minerals, can be considered a good platform for designing nanocomposite biomaterials for potential biomedical and active food packaging applications...
PB  - Univerzitet u Beogradu, Tehnološko-metalurški fakultet
T1  - Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka
T1  - Production and characterization of non-mineralized and mineralized biomaterials based on seaweed polysaccharides and essential metal zinc
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9303
ER  - 

@phdthesis{
author = "Malagurski, Ivana",
year = "2017",
abstract = "U ovoj tezi je ispitana mogućnost sinteze novih, poboljšanih biomaterijala na bazi alginata, agara i cinka, primenom biomimetičkog principa dizajna – biomineralizacije. Cilj istraživanja je bio dizajn i karakterizacija novih nanokompozitnih biomaterijala, sa potencijalnom primenom u biomedicini i pakovanju hrane..., The aim of this study was to test the hypothesis whether biopolymer-based biomineralization, with essential metal salts, Zn-minerals, can be considered a good platform for designing nanocomposite biomaterials for potential biomedical and active food packaging applications...",
publisher = "Univerzitet u Beogradu, Tehnološko-metalurški fakultet",
title = "Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka, Production and characterization of non-mineralized and mineralized biomaterials based on seaweed polysaccharides and essential metal zinc",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9303"
}

Malagurski, I.. (2017). Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka. 
Univerzitet u Beogradu, Tehnološko-metalurški fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_9303

Malagurski I. Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_9303 .

Malagurski, Ivana, "Dobijanje i karakterizacija nemineralizovanih i mineralizovanih biomaterijala na bazi polisaharida morskih algi i esencijalnog metala cinka" (2017),
https://hdl.handle.net/21.15107/rcub_nardus_9303 .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Nesić, Aleksandra
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/995
AB  - New mineralized, agar-based nanocomposite films (Zn-carbonate and Zn-phosphate/agar) were produced by a combination of in situ precipitation and a casting method. The presence of minerals significantly influenced the morphology, properties and functionality of the obtained nanocomposites. Reinforcement with the Zn-mineral phase improved the mechanical properties of the carbonate-mineralized films, but had a negligible effect on the phosphate-mineralized samples. Both nanocomposites showed improved optical and thermal properties, better Zn(II) release potential in a slightly acidic environment and exhibited antimicrobial activity against S. aureus. These results suggest that Zn-mineralized agar nanocomposite films could be potentially used as affordable, eco-friendly and active food packaging materials.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties
EP  - 62
SP  - 55
VL  - 175
DO  - 10.1016/j.carbpol.2017.07.064
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Nesić, Aleksandra and Mitrić, Miodrag and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "New mineralized, agar-based nanocomposite films (Zn-carbonate and Zn-phosphate/agar) were produced by a combination of in situ precipitation and a casting method. The presence of minerals significantly influenced the morphology, properties and functionality of the obtained nanocomposites. Reinforcement with the Zn-mineral phase improved the mechanical properties of the carbonate-mineralized films, but had a negligible effect on the phosphate-mineralized samples. Both nanocomposites showed improved optical and thermal properties, better Zn(II) release potential in a slightly acidic environment and exhibited antimicrobial activity against S. aureus. These results suggest that Zn-mineralized agar nanocomposite films could be potentially used as affordable, eco-friendly and active food packaging materials.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties",
pages = "62-55",
volume = "175",
doi = "10.1016/j.carbpol.2017.07.064"
}

Malagurski, I., Lević, S., Nesić, A., Mitrić, M., Pavlović, V.,& Dimitrijević-Branković, S.. (2017). Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 175, 55-62.
https://doi.org/10.1016/j.carbpol.2017.07.064

Malagurski I, Lević S, Nesić A, Mitrić M, Pavlović V, Dimitrijević-Branković S. Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties. in Carbohydrate Polymers. 2017;175:55-62.
doi:10.1016/j.carbpol.2017.07.064 .

Malagurski, Ivana, Lević, Steva, Nesić, Aleksandra, Mitrić, Miodrag, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Mineralized agar-based nanocomposite films: Potential food packaging materials with antimicrobial properties" in Carbohydrate Polymers, 175 (2017):55-62,
https://doi.org/10.1016/j.carbpol.2017.07.064 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Lević, Steva
AU  - Pantić, Milena
AU  - Matijašević, Danka
AU  - Mitrić, Miodrag
AU  - Pavlović, Vladimir
AU  - Dimitrijević-Branković, Suzana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1054
AB  - New bioactive and antimicrobial biomaterials were produced by alginate-mediated biomineralization with Zn-mineral phase. The synthesis procedure is simple, cost-effective and resulted in. two different Zn-mineralized alginate nanocomposites, Zn-carbonate/Zn-alginate and Zn-phosphate/Zn-alginate. The presence of Zn-mineral phase and its type, have significantly affected nanocomposite morphology, stability, total metallic loading and potential to release Zn(II) in physiological environment. Antimicrobial experiments showed that both types of Zn-mineralized nanocomposites exhibit strong antimicrobial effect against Escherichia coli, Staphylococcus aureus and Candida albicans. These results suggest that alginate biomineralization, where minerals are salts of essential metallic ions like Zn(II), represents a'good strategy for designing multifunctional biomaterials for potential biomedical applications.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites
EP  - 321
SP  - 313
VL  - 165
DO  - 10.1016/j.carbpol.2017.02.064
ER  - 

@article{
author = "Malagurski, Ivana and Lević, Steva and Pantić, Milena and Matijašević, Danka and Mitrić, Miodrag and Pavlović, Vladimir and Dimitrijević-Branković, Suzana",
year = "2017",
abstract = "New bioactive and antimicrobial biomaterials were produced by alginate-mediated biomineralization with Zn-mineral phase. The synthesis procedure is simple, cost-effective and resulted in. two different Zn-mineralized alginate nanocomposites, Zn-carbonate/Zn-alginate and Zn-phosphate/Zn-alginate. The presence of Zn-mineral phase and its type, have significantly affected nanocomposite morphology, stability, total metallic loading and potential to release Zn(II) in physiological environment. Antimicrobial experiments showed that both types of Zn-mineralized nanocomposites exhibit strong antimicrobial effect against Escherichia coli, Staphylococcus aureus and Candida albicans. These results suggest that alginate biomineralization, where minerals are salts of essential metallic ions like Zn(II), represents a'good strategy for designing multifunctional biomaterials for potential biomedical applications.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites",
pages = "321-313",
volume = "165",
doi = "10.1016/j.carbpol.2017.02.064"
}

Malagurski, I., Lević, S., Pantić, M., Matijašević, D., Mitrić, M., Pavlović, V.,& Dimitrijević-Branković, S.. (2017). Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 165, 313-321.
https://doi.org/10.1016/j.carbpol.2017.02.064

Malagurski I, Lević S, Pantić M, Matijašević D, Mitrić M, Pavlović V, Dimitrijević-Branković S. Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites. in Carbohydrate Polymers. 2017;165:313-321.
doi:10.1016/j.carbpol.2017.02.064 .

Malagurski, Ivana, Lević, Steva, Pantić, Milena, Matijašević, Danka, Mitrić, Miodrag, Pavlović, Vladimir, Dimitrijević-Branković, Suzana, "Synthesis and antimicrobial properties of Zn-mineralized alginate nanocomposites" in Carbohydrate Polymers, 165 (2017):313-321,
https://doi.org/10.1016/j.carbpol.2017.02.064 . .

TY  - JOUR
AU  - Kostić, Danijela D.
AU  - Malagurski, Ivana
AU  - Obradović, Bojana M.
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1037
AB  - The aim of this work was to assess phenomena occurring during AgNP transport from nanocomposite Ag/alginate hydrogels under conditions relevant for potential biomedical applications as antimicrobial soft tissue implants. First, we have studied AgNP migration from the nanocomposite to the adjacent alginate hydrogel mimicking soft tissue next to the implant. AgNP deposition was carried out by the initial burst release lasting for similar to 24 h yielding large aggregates on hydrogel surfaces and smaller clusters (similar to 400 nm in size) inside. However, the overall released content was low (0.67%) indicating high nanocomposite stability. In the next experimental series, release of AgNPs, 10-30 nm in size, from Ag/alginate microbeads in water was investigated under static conditions as well as under continuous perfusion mimicking vascularized tissues. Mathematical modeling has revealed AgNP release by diffusion under static conditions with the diffusion coefficient within the Ag/alginate hydrogel of 6.9x10(-19) m(2) s(-1). Conversely, continuous perfusion induced increased AgNP release by convection with the interstitial fluid velocity estimated as 4.6 nm s(-1). Overall, the obtained results indicated the influence of hydrodynamic conditions at the implantation site on silver release and potential implant functionality, which should be investigated at the experimentation beginning using appropriate in vitro systems.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation
EP  - 394
IS  - 5
SP  - 383
VL  - 71
DO  - 10.2298/HEMIND160713049K
ER  - 

@article{
author = "Kostić, Danijela D. and Malagurski, Ivana and Obradović, Bojana M.",
year = "2017",
abstract = "The aim of this work was to assess phenomena occurring during AgNP transport from nanocomposite Ag/alginate hydrogels under conditions relevant for potential biomedical applications as antimicrobial soft tissue implants. First, we have studied AgNP migration from the nanocomposite to the adjacent alginate hydrogel mimicking soft tissue next to the implant. AgNP deposition was carried out by the initial burst release lasting for similar to 24 h yielding large aggregates on hydrogel surfaces and smaller clusters (similar to 400 nm in size) inside. However, the overall released content was low (0.67%) indicating high nanocomposite stability. In the next experimental series, release of AgNPs, 10-30 nm in size, from Ag/alginate microbeads in water was investigated under static conditions as well as under continuous perfusion mimicking vascularized tissues. Mathematical modeling has revealed AgNP release by diffusion under static conditions with the diffusion coefficient within the Ag/alginate hydrogel of 6.9x10(-19) m(2) s(-1). Conversely, continuous perfusion induced increased AgNP release by convection with the interstitial fluid velocity estimated as 4.6 nm s(-1). Overall, the obtained results indicated the influence of hydrodynamic conditions at the implantation site on silver release and potential implant functionality, which should be investigated at the experimentation beginning using appropriate in vitro systems.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation",
pages = "394-383",
number = "5",
volume = "71",
doi = "10.2298/HEMIND160713049K"
}

Kostić, D. D., Malagurski, I.,& Obradović, B. M.. (2017). Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 71(5), 383-394.
https://doi.org/10.2298/HEMIND160713049K

Kostić DD, Malagurski I, Obradović BM. Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation. in Hemijska Industrija. 2017;71(5):383-394.
doi:10.2298/HEMIND160713049K .

Kostić, Danijela D., Malagurski, Ivana, Obradović, Bojana M., "Transport of silver nanoparticles from nanocomposite Ag/alginate hydrogels under conditions mimicking tissue implantation" in Hemijska Industrija, 71, no. 5 (2017):383-394,
https://doi.org/10.2298/HEMIND160713049K . .

TY  - JOUR
AU  - Bassett, David C.
AU  - Malagurski, Ivana
AU  - Beckwith, Kai S.
AU  - Melo, Thor Bernt
AU  - Obradović, Bojana
AU  - Sikorski, Pawel
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/892
AB  - Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive and controversial growth factors for applications in tissue engineering. Precise control and sustained release of copper ions are important due to a narrow therapeutic window of this potentially toxic ion, and alginate would appear to be a good material of choice for this purpose. We found that aqueously insoluble copper minerals could be precipitated during gelling within or mixed into alginate hydrogels in the form of microbeads prior to gelling to serve as depots of copper. These minerals were found to be soluble in a variety of biological fluids relevant to in vitro and in vivo investigations, and the alginate carrier served as a barrier to diffusion of these ions and therefore offered control over the rate and duration of release (Cu2+ release rates observed between 10-750 mu Mol g(-1) h(-1) and duration for up to 32 d). Copper mineral and copper mineralized alginate microbeads were characterized using powder x-ray diffraction, FTIR, thermogravimetric analysis and scanning electron microscopy. Dissolution kinetics were studied based on measurements of copper ion concentrations using colourimetric methods. In addition we characterized the complexes formed between released copper ions and biological fluids by electron paramagnetic spectroscopy which offers an insight into the behaviour of these materials in the body.
PB  - IOP Publishing Ltd, Bristol
T2  - Biomedical Materials
T1  - Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels
IS  - 1
VL  - 10
DO  - 10.1088/1748-6041/10/1/015006
ER  - 

@article{
author = "Bassett, David C. and Malagurski, Ivana and Beckwith, Kai S. and Melo, Thor Bernt and Obradović, Bojana and Sikorski, Pawel",
year = "2015",
abstract = "Here we investigate the dissolution behaviour of copper minerals contained within biocompatible alginate hydrogels. Copper has a number of biological effects and has most recently been evaluated as an alternative to expensive and controversial growth factors for applications in tissue engineering. Precise control and sustained release of copper ions are important due to a narrow therapeutic window of this potentially toxic ion, and alginate would appear to be a good material of choice for this purpose. We found that aqueously insoluble copper minerals could be precipitated during gelling within or mixed into alginate hydrogels in the form of microbeads prior to gelling to serve as depots of copper. These minerals were found to be soluble in a variety of biological fluids relevant to in vitro and in vivo investigations, and the alginate carrier served as a barrier to diffusion of these ions and therefore offered control over the rate and duration of release (Cu2+ release rates observed between 10-750 mu Mol g(-1) h(-1) and duration for up to 32 d). Copper mineral and copper mineralized alginate microbeads were characterized using powder x-ray diffraction, FTIR, thermogravimetric analysis and scanning electron microscopy. Dissolution kinetics were studied based on measurements of copper ion concentrations using colourimetric methods. In addition we characterized the complexes formed between released copper ions and biological fluids by electron paramagnetic spectroscopy which offers an insight into the behaviour of these materials in the body.",
publisher = "IOP Publishing Ltd, Bristol",
journal = "Biomedical Materials",
title = "Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels",
number = "1",
volume = "10",
doi = "10.1088/1748-6041/10/1/015006"
}

Bassett, D. C., Malagurski, I., Beckwith, K. S., Melo, T. B., Obradović, B.,& Sikorski, P.. (2015). Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels. in Biomedical Materials
IOP Publishing Ltd, Bristol., 10(1).
https://doi.org/10.1088/1748-6041/10/1/015006

Bassett DC, Malagurski I, Beckwith KS, Melo TB, Obradović B, Sikorski P. Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels. in Biomedical Materials. 2015;10(1).
doi:10.1088/1748-6041/10/1/015006 .

Bassett, David C., Malagurski, Ivana, Beckwith, Kai S., Melo, Thor Bernt, Obradović, Bojana, Sikorski, Pawel, "Dissolution of copper mineral phases in biological fluids and the controlled release of copper ions from mineralized alginate hydrogels" in Biomedical Materials, 10, no. 1 (2015),
https://doi.org/10.1088/1748-6041/10/1/015006 . .