TY  - CONF
AU  - Rajesekhran, Divya
AU  - Ponnupandian, Siva
AU  - Bhagabati, Purabi
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Babu, Ramesh
PY  - 2023
UR  - https://pps2023india.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2286
AB  - In a world where unsustainable anthropogenic activities are drastically affecting Earth’s
ecosystems and natural resources, threatening animal (including human) health and safety, the
sustainable management and use of resources, including preventing plastic pollution, have
become of paramount importance. Plastics, being at the core of many low cost and high
performing materials, are widely used despite being based on non-renewable petrochemicals.
Currently, in EU, only 7% of plastics are recycled out of 57.2 million tonnes produced, and
93% end up in incineration, landfills and oceans, releasing persistent, bio-accumulative, toxic
and hazardous chemicals.
Bio Innovation of a Circular Economy for Plastics 1 (BioICEP) an EU Horizon project focuses
on reducing the burden of plastic waste in the environment and also changing the linear value
chains of current plastic products. The project's overall objective is to demonstrate a seamless,
sustainable route to a circular economy for plastics by developing cost-effective and low-
energy demand processes for waste plastic biotransformation into high-value marketable
products such as biodegradable plastics. To address these challenges, the BioICEP project
focuses on developing physical, enzymatic and biocatalytic pre-treatments to convert the
mixed plastic waste to biodegradable plastics focusing on recycling and upcycling of the
products with a zero-waste and regenerative approach.
C3  - Polymer Processing Society Asia-Australasia Regional Conference
T1  - Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2286
ER  - 

@conference{
author = "Rajesekhran, Divya and Ponnupandian, Siva and Bhagabati, Purabi and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Babu, Ramesh",
year = "2023",
abstract = "In a world where unsustainable anthropogenic activities are drastically affecting Earth’s
ecosystems and natural resources, threatening animal (including human) health and safety, the
sustainable management and use of resources, including preventing plastic pollution, have
become of paramount importance. Plastics, being at the core of many low cost and high
performing materials, are widely used despite being based on non-renewable petrochemicals.
Currently, in EU, only 7% of plastics are recycled out of 57.2 million tonnes produced, and
93% end up in incineration, landfills and oceans, releasing persistent, bio-accumulative, toxic
and hazardous chemicals.
Bio Innovation of a Circular Economy for Plastics 1 (BioICEP) an EU Horizon project focuses
on reducing the burden of plastic waste in the environment and also changing the linear value
chains of current plastic products. The project's overall objective is to demonstrate a seamless,
sustainable route to a circular economy for plastics by developing cost-effective and low-
energy demand processes for waste plastic biotransformation into high-value marketable
products such as biodegradable plastics. To address these challenges, the BioICEP project
focuses on developing physical, enzymatic and biocatalytic pre-treatments to convert the
mixed plastic waste to biodegradable plastics focusing on recycling and upcycling of the
products with a zero-waste and regenerative approach.",
journal = "Polymer Processing Society Asia-Australasia Regional Conference",
title = "Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2286"
}

Rajesekhran, D., Ponnupandian, S., Bhagabati, P., Milovanović, J., Nikodinović-Runić, J.,& Babu, R.. (2023). Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics. in Polymer Processing Society Asia-Australasia Regional Conference.
https://hdl.handle.net/21.15107/rcub_imagine_2286

Rajesekhran D, Ponnupandian S, Bhagabati P, Milovanović J, Nikodinović-Runić J, Babu R. Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics. in Polymer Processing Society Asia-Australasia Regional Conference. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2286 .

Rajesekhran, Divya, Ponnupandian, Siva, Bhagabati, Purabi, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Babu, Ramesh, "Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics" in Polymer Processing Society Asia-Australasia Regional Conference (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2286 .

TY  - JOUR
AU  - Pekmezović, Marina
AU  - Krusić, Melina Kalagasidis
AU  - Malagurski, Ivana
AU  - Milovanović, Jelena
AU  - Stepien, Karolina
AU  - Guzik, Maciej
AU  - Charifou, Romina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1440
AB  - Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency
IS  - 6
VL  - 10
DO  - 10.3390/antibiotics10060737
ER  - 

@article{
author = "Pekmezović, Marina and Krusić, Melina Kalagasidis and Malagurski, Ivana and Milovanović, Jelena and Stepien, Karolina and Guzik, Maciej and Charifou, Romina and Babu, Ramesh and O'Connor, Kevin and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Novel biodegradable and biocompatible formulations of "old" but "gold" drugs such as nystatin (Nys) and amphotericin B (AmB) were made using a biopolymer as a matrix. Medium chain length polyhydroxyalkanoates (mcl-PHA) were used to formulate both polyenes (Nys and AmB) in the form of films (similar to 50 mu m). Thermal properties and stability of the materials were not significantly altered by the incorporation of polyenes in mcl-PHA, but polyene containing materials were more hydrophobic. These formulations were tested in vitro against a panel of pathogenic fungi and for antibiofilm properties. The films containing 0.1 to 2 weight % polyenes showed good activity and sustained polyene release for up to 4 days. A PHA monomer, namely 3-hydroxydecanoic acid (C10-OH), was added to the films to achieve an enhanced synergistic effect with polyenes against fungal growth. Mcl-PHA based polyene formulations showed excellent growth inhibitory activity against both Candida yeasts (C. albicans ATCC 1023, C. albicans SC5314 (ATCC MYA-2876), C. parapsilosis ATCC 22019) and filamentous fungi (Aspergillus fumigatus ATCC 13073; Trichophyton mentagrophytes ATCC 9533, Microsporum gypseum ATCC 24102). All antifungal PHA film preparations prevented the formation of a C. albicans biofilm, while they were not efficient in eradication of mature biofilms, rendering them suitable for the transdermal application or as coatings of implants.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency",
number = "6",
volume = "10",
doi = "10.3390/antibiotics10060737"
}

Pekmezović, M., Krusić, M. K., Malagurski, I., Milovanović, J., Stepien, K., Guzik, M., Charifou, R., Babu, R., O'Connor, K.,& Nikodinović-Runić, J.. (2021). Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel
MDPI, Basel., 10(6).
https://doi.org/10.3390/antibiotics10060737

Pekmezović M, Krusić MK, Malagurski I, Milovanović J, Stepien K, Guzik M, Charifou R, Babu R, O'Connor K, Nikodinović-Runić J. Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency. in Antibiotics-Basel. 2021;10(6).
doi:10.3390/antibiotics10060737 .

Pekmezović, Marina, Krusić, Melina Kalagasidis, Malagurski, Ivana, Milovanović, Jelena, Stepien, Karolina, Guzik, Maciej, Charifou, Romina, Babu, Ramesh, O'Connor, Kevin, Nikodinović-Runić, Jasmina, "Polyhydroxyalkanoate/Antifungal Polyene Formulations with Monomeric Hydroxyalkanoic Acids for Improved Antifungal Efficiency" in Antibiotics-Basel, 10, no. 6 (2021),
https://doi.org/10.3390/antibiotics10060737 . .

TY  - JOUR
AU  - Nikolaivits, Efstratios
AU  - Pantelić, Brana
AU  - Azeem, Muhammad
AU  - Taxeidis, George
AU  - Babu, Ramesh
AU  - Topakas, Evangelos
AU  - Fournet, Margaret Brennan
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1479
AB  - Inspirational concepts, and the transfer of analogs from natural biology to science and engineering, has produced many excellent technologies to date, spanning vaccines to modern architectural feats. This review highlights that answers to the pressing global petroleum-based plastic waste challenges, can be found within the mechanics and mechanisms natural ecosystems. Here, a suite of technological and engineering approaches, which can be implemented to operate in tandem with nature's prescription for regenerative material circularity, is presented as a route to plastics sustainability. A number of mechanical/green chemical (pre)treatment methodologies, which simulate natural weathering and arthropodal dismantling activities are reviewed, including: mechanical milling, reactive extrusion, ultrasonic-, UV- and degradation using supercritical CO2. Akin to natural mechanical degradation, the purpose of the pretreatments is to render the plastic materials more amenable to microbial and biocatalytic activities, to yield effective depolymerization and (re)valorization. While biotechnological based degradation and depolymerization of both recalcitrant and bioplastics are at a relatively early stage of development, the potential for acceleration and expedition of valuable output monomers and oligomers yields is considerable. To date a limited number of independent mechano-green chemical approaches and a considerable and growing number of standalone enzymatic and microbial degradation studies have been reported. A convergent strategy, one which forges mechano-green chemical treatments together with the enzymatic and microbial actions, is largely lacking at this time. An overview of the reported microbial and enzymatic degradations of petroleum-based synthetic polymer plastics, specifically: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polystyrene (PS), polyethylene terephthalate (PET), polyurethanes (PU) and polycaprolactone (PCL) and selected prevalent bio-based or bio-polymers [polylactic acid (PLA), polyhydroxyalkanoates (PHAs) and polybutylene succinate (PBS)], is detailed. The harvesting of depolymerization products to produce new materials and higher-value products is also a key endeavor in effectively completing the circle for plastics. Our challenge is now to effectively combine and conjugate the requisite cross disciplinary approaches and progress the essential science and engineering technologies to categorically complete the life-cycle for plastics.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization
VL  - 9
DO  - 10.3389/fbioe.2021.696040
ER  - 

@article{
author = "Nikolaivits, Efstratios and Pantelić, Brana and Azeem, Muhammad and Taxeidis, George and Babu, Ramesh and Topakas, Evangelos and Fournet, Margaret Brennan and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Inspirational concepts, and the transfer of analogs from natural biology to science and engineering, has produced many excellent technologies to date, spanning vaccines to modern architectural feats. This review highlights that answers to the pressing global petroleum-based plastic waste challenges, can be found within the mechanics and mechanisms natural ecosystems. Here, a suite of technological and engineering approaches, which can be implemented to operate in tandem with nature's prescription for regenerative material circularity, is presented as a route to plastics sustainability. A number of mechanical/green chemical (pre)treatment methodologies, which simulate natural weathering and arthropodal dismantling activities are reviewed, including: mechanical milling, reactive extrusion, ultrasonic-, UV- and degradation using supercritical CO2. Akin to natural mechanical degradation, the purpose of the pretreatments is to render the plastic materials more amenable to microbial and biocatalytic activities, to yield effective depolymerization and (re)valorization. While biotechnological based degradation and depolymerization of both recalcitrant and bioplastics are at a relatively early stage of development, the potential for acceleration and expedition of valuable output monomers and oligomers yields is considerable. To date a limited number of independent mechano-green chemical approaches and a considerable and growing number of standalone enzymatic and microbial degradation studies have been reported. A convergent strategy, one which forges mechano-green chemical treatments together with the enzymatic and microbial actions, is largely lacking at this time. An overview of the reported microbial and enzymatic degradations of petroleum-based synthetic polymer plastics, specifically: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polystyrene (PS), polyethylene terephthalate (PET), polyurethanes (PU) and polycaprolactone (PCL) and selected prevalent bio-based or bio-polymers [polylactic acid (PLA), polyhydroxyalkanoates (PHAs) and polybutylene succinate (PBS)], is detailed. The harvesting of depolymerization products to produce new materials and higher-value products is also a key endeavor in effectively completing the circle for plastics. Our challenge is now to effectively combine and conjugate the requisite cross disciplinary approaches and progress the essential science and engineering technologies to categorically complete the life-cycle for plastics.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization",
volume = "9",
doi = "10.3389/fbioe.2021.696040"
}

Nikolaivits, E., Pantelić, B., Azeem, M., Taxeidis, G., Babu, R., Topakas, E., Fournet, M. B.,& Nikodinović-Runić, J.. (2021). Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fbioe.2021.696040

Nikolaivits E, Pantelić B, Azeem M, Taxeidis G, Babu R, Topakas E, Fournet MB, Nikodinović-Runić J. Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization. in Frontiers in Bioengineering and Biotechnology. 2021;9.
doi:10.3389/fbioe.2021.696040 .

Nikolaivits, Efstratios, Pantelić, Brana, Azeem, Muhammad, Taxeidis, George, Babu, Ramesh, Topakas, Evangelos, Fournet, Margaret Brennan, Nikodinović-Runić, Jasmina, "Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization" in Frontiers in Bioengineering and Biotechnology, 9 (2021),
https://doi.org/10.3389/fbioe.2021.696040 . .

TY  - JOUR
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani K.
AU  - Neels, Antonia
AU  - Anđelković, Boban
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh
AU  - O'Connor, Kevin 
AU  - Witko, Tomasz
AU  - Solarz, Daria
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1481
AB  - New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.
PB  - Elsevier, Amsterdam
T2  - Materials Letters
T1  - Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application
VL  - 285
DO  - 10.1016/j.matlet.2020.129100
ER  - 

@article{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani K. and Neels, Antonia and Anđelković, Boban and Senthamaraikannan, Ramsankar and Babu, Ramesh and O'Connor, Kevin  and Witko, Tomasz and Solarz, Daria and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "New polyhydroxyoctanoate based composites with incorporated TiO2 microfibers were produced. The presence of the inorganic constituent influenced morphology, physical properties and functionality of the obtained biomaterials. The degree of PHO crystallinity decreased in the composites in a TiO2 concentration dependent manner. The composites were stiffer than the neat PHO, however they preserved their flexibility. Biocompatibility and cellular migration studies showed that composites support cell viability and migration. The obtained results suggest that PHO/TiO2 composites could be used as novel biomaterials with tunable properties for biomedical applications.",
publisher = "Elsevier, Amsterdam",
journal = "Materials Letters",
title = "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application",
volume = "285",
doi = "10.1016/j.matlet.2020.129100"
}

Malagurski, I., Frison, R., Maurya, A. K., Neels, A., Anđelković, B., Senthamaraikannan, R., Babu, R., O'Connor, K., Witko, T., Solarz, D.,& Nikodinović-Runić, J.. (2021). Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters
Elsevier, Amsterdam., 285.
https://doi.org/10.1016/j.matlet.2020.129100

Malagurski I, Frison R, Maurya AK, Neels A, Anđelković B, Senthamaraikannan R, Babu R, O'Connor K, Witko T, Solarz D, Nikodinović-Runić J. Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application. in Materials Letters. 2021;285.
doi:10.1016/j.matlet.2020.129100 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani K., Neels, Antonia, Anđelković, Boban, Senthamaraikannan, Ramsankar, Babu, Ramesh, O'Connor, Kevin , Witko, Tomasz, Solarz, Daria, Nikodinović-Runić, Jasmina, "Polyhydroxyoctanoate films reinforced with titanium dioxide microfibers for biomedical application" in Materials Letters, 285 (2021),
https://doi.org/10.1016/j.matlet.2020.129100 . .

TY  - JOUR
AU  - Simić, Stefan
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Bozić, Nataša
AU  - Vujcić, Zoran
AU  - Loncar, Nikola
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh
AU  - Opsenica, Igor M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1406
AB  - Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7-24 h with good yields (70-99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 degrees C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.
PB  - New York : Elsevier Science Inc
T2  - Enzyme and Microbial Technology
T1  - Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
VL  - 132
DO  - 10.1016/j.enzmictec.2019.109411
ER  - 

@article{
author = "Simić, Stefan and Jeremić, Sanja and Đokić, Lidija and Bozić, Nataša and Vujcić, Zoran and Loncar, Nikola and Senthamaraikannan, Ramsankar and Babu, Ramesh and Opsenica, Igor M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7-24 h with good yields (70-99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 degrees C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.",
publisher = "New York : Elsevier Science Inc",
journal = "Enzyme and Microbial Technology",
title = "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines",
volume = "132",
doi = "10.1016/j.enzmictec.2019.109411"
}

Simić, S., Jeremić, S., Đokić, L., Bozić, N., Vujcić, Z., Loncar, N., Senthamaraikannan, R., Babu, R., Opsenica, I. M.,& Nikodinović-Runić, J.. (2020). Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology
New York : Elsevier Science Inc., 132.
https://doi.org/10.1016/j.enzmictec.2019.109411

Simić S, Jeremić S, Đokić L, Bozić N, Vujcić Z, Loncar N, Senthamaraikannan R, Babu R, Opsenica IM, Nikodinović-Runić J. Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology. 2020;132.
doi:10.1016/j.enzmictec.2019.109411 .

Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Bozić, Nataša, Vujcić, Zoran, Loncar, Nikola, Senthamaraikannan, Ramsankar, Babu, Ramesh, Opsenica, Igor M., Nikodinović-Runić, Jasmina, "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines" in Enzyme and Microbial Technology, 132 (2020),
https://doi.org/10.1016/j.enzmictec.2019.109411 . .

TY  - CONF
AU  - Malagurski, Ivana
AU  - Frison, Ruggero
AU  - Maurya, Anjani
AU  - Nikodinović-Runić, Jasmina
AU  - Babu, Ramesh
AU  - O'Connor, Kevin
AU  - Neels, Antonia
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1216
AB  - Medium-chain polyhydroxyalkanoates (mcl-PHA) are flexible, elastomeric polymers produced by wide range of
bacteria as intercellular storage of carbon and energy. They represent attractive components in biomaterial design
because they are biocompatible, biodegradable and can be obtained using variety of carbon sources including
waste streams[1]. However, being semi-crystalline, all mcl-PHAs are characterized by low melting temperature and
poor tensile strength which can interfere with processing methods and wider biomedical application. Simple way
to improve mcl-PHAs properties is to incorporate a nanophase within biopolymer to obtain nanocomposites.
Nano-sized constituents interact with biopolymer more intimately affecting in turn the obtained nanocomposite
properties as well as functionality. Among inorganic nanofillers, TiO2 nanostructures with high aspect ratio (e.g.
nanofibers) have unique properties that support osteogenic phenotype which makes them suitable for bone tissue
engineering [2].
PB  - Int Union Crystallography, Chester
C3  - Acta Crystallographica A-Foundation and Advances
T1  - Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd
VL  - 75
DO  - 10.1107/S2053273319089794
ER  - 

@conference{
author = "Malagurski, Ivana and Frison, Ruggero and Maurya, Anjani and Nikodinović-Runić, Jasmina and Babu, Ramesh and O'Connor, Kevin and Neels, Antonia",
year = "2019",
abstract = "Medium-chain polyhydroxyalkanoates (mcl-PHA) are flexible, elastomeric polymers produced by wide range of
bacteria as intercellular storage of carbon and energy. They represent attractive components in biomaterial design
because they are biocompatible, biodegradable and can be obtained using variety of carbon sources including
waste streams[1]. However, being semi-crystalline, all mcl-PHAs are characterized by low melting temperature and
poor tensile strength which can interfere with processing methods and wider biomedical application. Simple way
to improve mcl-PHAs properties is to incorporate a nanophase within biopolymer to obtain nanocomposites.
Nano-sized constituents interact with biopolymer more intimately affecting in turn the obtained nanocomposite
properties as well as functionality. Among inorganic nanofillers, TiO2 nanostructures with high aspect ratio (e.g.
nanofibers) have unique properties that support osteogenic phenotype which makes them suitable for bone tissue
engineering [2].",
publisher = "Int Union Crystallography, Chester",
journal = "Acta Crystallographica A-Foundation and Advances",
title = "Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd",
volume = "75",
doi = "10.1107/S2053273319089794"
}

Malagurski, I., Frison, R., Maurya, A., Nikodinović-Runić, J., Babu, R., O'Connor, K.,& Neels, A.. (2019). Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd. in Acta Crystallographica A-Foundation and Advances
Int Union Crystallography, Chester., 75.
https://doi.org/10.1107/S2053273319089794

Malagurski I, Frison R, Maurya A, Nikodinović-Runić J, Babu R, O'Connor K, Neels A. Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd. in Acta Crystallographica A-Foundation and Advances. 2019;75.
doi:10.1107/S2053273319089794 .

Malagurski, Ivana, Frison, Ruggero, Maurya, Anjani, Nikodinović-Runić, Jasmina, Babu, Ramesh, O'Connor, Kevin, Neels, Antonia, "Medium chain length (mcl)-pha-based nanocomposites for biomedical applications: system evaluation through xrd" in Acta Crystallographica A-Foundation and Advances, 75 (2019),
https://doi.org/10.1107/S2053273319089794 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Ajdačić, Vladimir
AU  - Bozinović, Nina
AU  - Pavlović, Vladimir
AU  - Manojlović, Dragan D.
AU  - Babu, Ramesh
AU  - Senthamaraikannan, Ramsankar
AU  - Rojas, Orlando
AU  - Opsenica, Igor
AU  - Nikodinović-Runić, Jasmina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1267
AB  - Bacterial nanocellulose (BNC) emerged as an attractive advanced biomaterial that provides desirable properties such as high strength, lightweight, tailorable surface chemistry, hydrophilicity, and biodegradability. BNC was successfully obtained from a wide range of carbon sources including sugars derived from grass biomass using Komagataeibacter medellinensis ID13488 strain with yields up to 6 g L-1 in static fermentation. Produced BNC was utilized in straightforward catalyst preparation as a solid support for two different transition metals, palladium and copper with metal loading of 20 and 3 wt%, respectively. Sustainable catalysts were applied in the synthesis of valuable fine chemicals, such as biphenyl-4-amine and 4'-fluorobiphenyl-4-amine, used in drug discovery, perfumes and dye industries with excellent product yields of up to 99%. Pd/BNC catalyst was reused 4 times and applied in two consecutive reactions, Suzuki-Miyaura cross-coupling reaction followed by hydrogenation of nitro to amino group while Cu/BNC catalyst was examined in Chan-Lam coupling reaction. Overall, the environmentally benign process of obtaining nanocellulose from biomass, followed by its utilisation as a solid support in metal-catalysed reactions and its recovery has been described. These findings reveal that BNC is a good support material, and it can be used as a support for different catalytic systems.
PB  - Elsevier, Amsterdam
T2  - International Journal of Biological Macromolecules
T1  - Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions
EP  - 360
SP  - 351
VL  - 129
DO  - 10.1016/j.ijbiomac.2019.01.154
ER  - 

@article{
author = "Jeremić, Sanja and Đokić, Lidija and Ajdačić, Vladimir and Bozinović, Nina and Pavlović, Vladimir and Manojlović, Dragan D. and Babu, Ramesh and Senthamaraikannan, Ramsankar and Rojas, Orlando and Opsenica, Igor and Nikodinović-Runić, Jasmina",
year = "2019",
abstract = "Bacterial nanocellulose (BNC) emerged as an attractive advanced biomaterial that provides desirable properties such as high strength, lightweight, tailorable surface chemistry, hydrophilicity, and biodegradability. BNC was successfully obtained from a wide range of carbon sources including sugars derived from grass biomass using Komagataeibacter medellinensis ID13488 strain with yields up to 6 g L-1 in static fermentation. Produced BNC was utilized in straightforward catalyst preparation as a solid support for two different transition metals, palladium and copper with metal loading of 20 and 3 wt%, respectively. Sustainable catalysts were applied in the synthesis of valuable fine chemicals, such as biphenyl-4-amine and 4'-fluorobiphenyl-4-amine, used in drug discovery, perfumes and dye industries with excellent product yields of up to 99%. Pd/BNC catalyst was reused 4 times and applied in two consecutive reactions, Suzuki-Miyaura cross-coupling reaction followed by hydrogenation of nitro to amino group while Cu/BNC catalyst was examined in Chan-Lam coupling reaction. Overall, the environmentally benign process of obtaining nanocellulose from biomass, followed by its utilisation as a solid support in metal-catalysed reactions and its recovery has been described. These findings reveal that BNC is a good support material, and it can be used as a support for different catalytic systems.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Biological Macromolecules",
title = "Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions",
pages = "360-351",
volume = "129",
doi = "10.1016/j.ijbiomac.2019.01.154"
}

Jeremić, S., Đokić, L., Ajdačić, V., Bozinović, N., Pavlović, V., Manojlović, D. D., Babu, R., Senthamaraikannan, R., Rojas, O., Opsenica, I.,& Nikodinović-Runić, J.. (2019). Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions. in International Journal of Biological Macromolecules
Elsevier, Amsterdam., 129, 351-360.
https://doi.org/10.1016/j.ijbiomac.2019.01.154

Jeremić S, Đokić L, Ajdačić V, Bozinović N, Pavlović V, Manojlović DD, Babu R, Senthamaraikannan R, Rojas O, Opsenica I, Nikodinović-Runić J. Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions. in International Journal of Biological Macromolecules. 2019;129:351-360.
doi:10.1016/j.ijbiomac.2019.01.154 .

Jeremić, Sanja, Đokić, Lidija, Ajdačić, Vladimir, Bozinović, Nina, Pavlović, Vladimir, Manojlović, Dragan D., Babu, Ramesh, Senthamaraikannan, Ramsankar, Rojas, Orlando, Opsenica, Igor, Nikodinović-Runić, Jasmina, "Production of bacterial nanocellulose (BNC) and its application as a solid support in transition metal catalysed cross-coupling reactions" in International Journal of Biological Macromolecules, 129 (2019):351-360,
https://doi.org/10.1016/j.ijbiomac.2019.01.154 . .

TY  - JOUR
AU  - Cerrone, Federico
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Walsh, Meg
AU  - Kenny, Shane T.
AU  - Babu, Ramesh
AU  - O'Connor, Kevin 
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1199
AB  - A sodium alkyl disulfate mixture (SADM) synthesised from microbially produced 3-hydroxy fatty acids methyl esters (HFAMEs), showed 13-fold surface tension decrease when compared with the reference surfactant sodium dodecyl sulfate (SDS). Polyhydroxyalkanoates, accumulated by bacteria intracellularly when supplied with a mixture of fatty acids derived from hydrolysed rapeseed oil, were isolated, depolymerised and methylated to produce HFAMEs in very high yield (90%). A sequential chemical reduction and sulfation of the HFAMEs produced the sodium alkyl disulfates in high yields ( gt  65%). SADM performs also 1.3-times better than dodecyl (1,3) disulfate, in surface tension tests. SADM shows also the formation of a specific critical micelle concentration (CMC) at a concentration 21-fold lower than SDS. The wettability of the SADM mixture is similar to SDS but the foaming volume of SADM is 1.5-fold higher. The foam is also more stable with its volume decreasing 3 times slower over time compared to SDS at their respective CMC values. Established sulfation technologies in chemical manufacturing could use the 3-hydroxy fatty acids methyl esters moiety (3-HFAME) given its origin from rapeseed oil and the extra OH residue on 3-position in the molecule, which affords the opportunity to produce disulfate surfactants with a proven superior performance to monosulphated surfactants. Thus, not only addressing environmental issues by avoiding threats of deforestation and monocultivation associated with palm oil use but also achieve a higher performance with lower use of surfactants.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters
VL  - 182
DO  - 10.1016/j.colsurfb.2019.06.062
ER  - 

@article{
author = "Cerrone, Federico and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Walsh, Meg and Kenny, Shane T. and Babu, Ramesh and O'Connor, Kevin ",
year = "2019",
abstract = "A sodium alkyl disulfate mixture (SADM) synthesised from microbially produced 3-hydroxy fatty acids methyl esters (HFAMEs), showed 13-fold surface tension decrease when compared with the reference surfactant sodium dodecyl sulfate (SDS). Polyhydroxyalkanoates, accumulated by bacteria intracellularly when supplied with a mixture of fatty acids derived from hydrolysed rapeseed oil, were isolated, depolymerised and methylated to produce HFAMEs in very high yield (90%). A sequential chemical reduction and sulfation of the HFAMEs produced the sodium alkyl disulfates in high yields ( gt  65%). SADM performs also 1.3-times better than dodecyl (1,3) disulfate, in surface tension tests. SADM shows also the formation of a specific critical micelle concentration (CMC) at a concentration 21-fold lower than SDS. The wettability of the SADM mixture is similar to SDS but the foaming volume of SADM is 1.5-fold higher. The foam is also more stable with its volume decreasing 3 times slower over time compared to SDS at their respective CMC values. Established sulfation technologies in chemical manufacturing could use the 3-hydroxy fatty acids methyl esters moiety (3-HFAME) given its origin from rapeseed oil and the extra OH residue on 3-position in the molecule, which affords the opportunity to produce disulfate surfactants with a proven superior performance to monosulphated surfactants. Thus, not only addressing environmental issues by avoiding threats of deforestation and monocultivation associated with palm oil use but also achieve a higher performance with lower use of surfactants.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters",
volume = "182",
doi = "10.1016/j.colsurfb.2019.06.062"
}

Cerrone, F., Milovanović, J., Nikodinović-Runić, J., Walsh, M., Kenny, S. T., Babu, R.,& O'Connor, K.. (2019). Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 182.
https://doi.org/10.1016/j.colsurfb.2019.06.062

Cerrone F, Milovanović J, Nikodinović-Runić J, Walsh M, Kenny ST, Babu R, O'Connor K. Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters. in Colloids and Surfaces B-Biointerfaces. 2019;182.
doi:10.1016/j.colsurfb.2019.06.062 .

Cerrone, Federico, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Walsh, Meg, Kenny, Shane T., Babu, Ramesh, O'Connor, Kevin , "Novel sodium alkyl-1,3-disulfates, anionic biosurfactants produced from microbial polyesters" in Colloids and Surfaces B-Biointerfaces, 182 (2019),
https://doi.org/10.1016/j.colsurfb.2019.06.062 . .

TY  - JOUR
AU  - Guzik, Maciej W.
AU  - Narancić, Tanja
AU  - Ilić-Tomić, Tatjana
AU  - Vojnović, Sandra
AU  - Kenny, Shane T.
AU  - Casey, William T.
AU  - Duane, Gearoid F.
AU  - Casey, Eoin
AU  - Woods, Trevor
AU  - Babu, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Kevin 
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/749
AB  - Diverse and elaborate pathways for nutrient utilization, as well as mechanisms to combat unfavourable nutrient conditions make Pseudomonas putida KT2440 a versatile micro-organism able to occupy a range of ecological niches. The fatty acid degradation pathway of P. putida is complex and correlated with biopolymer medium chain length polyhydroxyalkanoate (mcl-PHA) biosynthesis. Little is known about the second step of fatty acid degradation (beta-oxidation) in this strain. In silico analysis of its genome sequence revealed 21 putative acyl-CoA dehydrogenases (ACADs), four of which were functionally characterized through mutagenesis studies. Four mutants with insertionally inactivated ACADs (PP_1893, PP_2039, PP_2048 and PP_2437) grew and accumulated mcl-PHA on a range of fatty acids as the sole source of carbon and energy. Their ability to grow and accumulate biopolymer was differentially negatively affected on various fatty acids, in comparison to the wild-type strain. Inactive PP_2437 exhibited a pattern of reduced growth and PHA accumulation when fatty acids with lengths of 10 to 14 carbon chains were used as substrates. Recombinant expression and biochemical characterization of the purified protein allowed functional annotation in P. putida KT2440 as an ACAD showing clear preference for dodecanoyl-CoA ester as a substrate and optimum activity at 30 degrees C and pH 6.5-7.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids
EP  - 1771
SP  - 1760
VL  - 160
DO  - 10.1099/mic.0.078758-0
ER  - 

@article{
author = "Guzik, Maciej W. and Narancić, Tanja and Ilić-Tomić, Tatjana and Vojnović, Sandra and Kenny, Shane T. and Casey, William T. and Duane, Gearoid F. and Casey, Eoin and Woods, Trevor and Babu, Ramesh and Nikodinović-Runić, Jasmina and O'Connor, Kevin ",
year = "2014",
abstract = "Diverse and elaborate pathways for nutrient utilization, as well as mechanisms to combat unfavourable nutrient conditions make Pseudomonas putida KT2440 a versatile micro-organism able to occupy a range of ecological niches. The fatty acid degradation pathway of P. putida is complex and correlated with biopolymer medium chain length polyhydroxyalkanoate (mcl-PHA) biosynthesis. Little is known about the second step of fatty acid degradation (beta-oxidation) in this strain. In silico analysis of its genome sequence revealed 21 putative acyl-CoA dehydrogenases (ACADs), four of which were functionally characterized through mutagenesis studies. Four mutants with insertionally inactivated ACADs (PP_1893, PP_2039, PP_2048 and PP_2437) grew and accumulated mcl-PHA on a range of fatty acids as the sole source of carbon and energy. Their ability to grow and accumulate biopolymer was differentially negatively affected on various fatty acids, in comparison to the wild-type strain. Inactive PP_2437 exhibited a pattern of reduced growth and PHA accumulation when fatty acids with lengths of 10 to 14 carbon chains were used as substrates. Recombinant expression and biochemical characterization of the purified protein allowed functional annotation in P. putida KT2440 as an ACAD showing clear preference for dodecanoyl-CoA ester as a substrate and optimum activity at 30 degrees C and pH 6.5-7.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids",
pages = "1771-1760",
volume = "160",
doi = "10.1099/mic.0.078758-0"
}

Guzik, M. W., Narancić, T., Ilić-Tomić, T., Vojnović, S., Kenny, S. T., Casey, W. T., Duane, G. F., Casey, E., Woods, T., Babu, R., Nikodinović-Runić, J.,& O'Connor, K.. (2014). Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids. in Microbiology-Sgm
Microbiology Soc, London., 160, 1760-1771.
https://doi.org/10.1099/mic.0.078758-0

Guzik MW, Narancić T, Ilić-Tomić T, Vojnović S, Kenny ST, Casey WT, Duane GF, Casey E, Woods T, Babu R, Nikodinović-Runić J, O'Connor K. Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids. in Microbiology-Sgm. 2014;160:1760-1771.
doi:10.1099/mic.0.078758-0 .

Guzik, Maciej W., Narancić, Tanja, Ilić-Tomić, Tatjana, Vojnović, Sandra, Kenny, Shane T., Casey, William T., Duane, Gearoid F., Casey, Eoin, Woods, Trevor, Babu, Ramesh, Nikodinović-Runić, Jasmina, O'Connor, Kevin , "Identification and characterization of an acyl-CoA dehydrogenase from Pseudomonas putida KT2440 that shows preference towards medium to long chain length fatty acids" in Microbiology-Sgm, 160 (2014):1760-1771,
https://doi.org/10.1099/mic.0.078758-0 . .

TY  - JOUR
AU  - Guzik, Maciej W.
AU  - Kenny, Shane T.
AU  - Duane, Gearoid F.
AU  - Casey, Eoin
AU  - Woods, Trevor
AU  - Babu, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - Murray, Michael
AU  - O'Connor, Kevin 
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/784
AB  - A process for the conversion of post consumer (agricultural) polyethylene (PE) waste to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) is reported here. The thermal treatment of PE in the absence of air (pyrolysis) generated a complex mixture of low molecular weight paraffins with carbon chain lengths from C8 to C32 (PE pyrolysis wax). Several bacterial strains were able to grow and produce PHA from this PE pyrolysis wax. The addition of biosurfactant (rhamnolipids) allowed for greater bacterial growth and PHA accumulation of the tested strains. Some strains were only capable of growth and PHA accumulation in the presence of the biosurfactant. Pseudomonas aeruginosa PAO-1 accumulated the highest level of PHA with almost 25 % of the cell dry weight as PHA when supplied with the PE pyrolysis wax in the presence of rhamnolipids. The change of nitrogen source from ammonium chloride to ammonium nitrate resulted in faster bacterial growth and the earlier onset of PHA accumulation. To our knowledge, this is the first report where PE is used as a starting material for production of a biodegradable polymer.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate
EP  - 4232
IS  - 9
SP  - 4223
VL  - 98
DO  - 10.1007/s00253-013-5489-2
ER  - 

@article{
author = "Guzik, Maciej W. and Kenny, Shane T. and Duane, Gearoid F. and Casey, Eoin and Woods, Trevor and Babu, Ramesh and Nikodinović-Runić, Jasmina and Murray, Michael and O'Connor, Kevin ",
year = "2014",
abstract = "A process for the conversion of post consumer (agricultural) polyethylene (PE) waste to the biodegradable polymer medium chain length polyhydroxyalkanoate (mcl-PHA) is reported here. The thermal treatment of PE in the absence of air (pyrolysis) generated a complex mixture of low molecular weight paraffins with carbon chain lengths from C8 to C32 (PE pyrolysis wax). Several bacterial strains were able to grow and produce PHA from this PE pyrolysis wax. The addition of biosurfactant (rhamnolipids) allowed for greater bacterial growth and PHA accumulation of the tested strains. Some strains were only capable of growth and PHA accumulation in the presence of the biosurfactant. Pseudomonas aeruginosa PAO-1 accumulated the highest level of PHA with almost 25 % of the cell dry weight as PHA when supplied with the PE pyrolysis wax in the presence of rhamnolipids. The change of nitrogen source from ammonium chloride to ammonium nitrate resulted in faster bacterial growth and the earlier onset of PHA accumulation. To our knowledge, this is the first report where PE is used as a starting material for production of a biodegradable polymer.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate",
pages = "4232-4223",
number = "9",
volume = "98",
doi = "10.1007/s00253-013-5489-2"
}

Guzik, M. W., Kenny, S. T., Duane, G. F., Casey, E., Woods, T., Babu, R., Nikodinović-Runić, J., Murray, M.,& O'Connor, K.. (2014). Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 98(9), 4223-4232.
https://doi.org/10.1007/s00253-013-5489-2

Guzik MW, Kenny ST, Duane GF, Casey E, Woods T, Babu R, Nikodinović-Runić J, Murray M, O'Connor K. Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2014;98(9):4223-4232.
doi:10.1007/s00253-013-5489-2 .

Guzik, Maciej W., Kenny, Shane T., Duane, Gearoid F., Casey, Eoin, Woods, Trevor, Babu, Ramesh, Nikodinović-Runić, Jasmina, Murray, Michael, O'Connor, Kevin , "Conversion of post consumer polyethylene to the biodegradable polymer polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 98, no. 9 (2014):4223-4232,
https://doi.org/10.1007/s00253-013-5489-2 . .

TY  - JOUR
AU  - O'Connor, Stephen
AU  - Szwej, Emilia
AU  - Nikodinović-Runić, Jasmina
AU  - O'Connor, Aisling
AU  - Byrne, Annette T.
AU  - Devocelle, Marc
AU  - O'Donovan, Norma
AU  - Gallagher, William M.
AU  - Babu, Ramesh
AU  - Kenny, Shane T.
AU  - Zinn, Manfred
AU  - Zulian, Qun Ren
AU  - O'Connor, Kevin 
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/637
AB  - The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values compared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range.
PB  - Elsevier Sci Ltd, Oxford
T2  - Biomaterials
T1  - The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate
EP  - 2718
IS  - 11
SP  - 2710
VL  - 34
DO  - 10.1016/j.biomaterials.2012.12.032
ER  - 

@article{
author = "O'Connor, Stephen and Szwej, Emilia and Nikodinović-Runić, Jasmina and O'Connor, Aisling and Byrne, Annette T. and Devocelle, Marc and O'Donovan, Norma and Gallagher, William M. and Babu, Ramesh and Kenny, Shane T. and Zinn, Manfred and Zulian, Qun Ren and O'Connor, Kevin ",
year = "2013",
abstract = "The biodegradable polymer medium chain length polyhydroxyalkanoate (mclPHA), produced by Pseudomonas putida CA-3, was depolymerised and the predominant monomer (R)-3-hydroxydecanoic acid (R10) purified. R10 was conjugated to a D-peptide DP18 and its derivatives. All peptides conjugated with R10 exhibited greater anti-cancer activity compared to the unconjugated peptides. Unconjugated and conjugated peptides were cytocidal for cancer cells. Conjugation of R10 to peptides was essential for enhanced anti-proliferation activity, as unconjugated mixes did not result in enhancement of anti-cancer activity. The conjugation of R10 resulted in more rapid uptake of peptides into HeLa and MiaPaCa cells compared to unconjugated peptide. Both unconjugated and R10 conjugated peptides localized to the mitochondria of HeLa and MiaPaCa cells and induced apoptosis. Peptide conjugated with a terminally hydroxylated decanoic acid (omega-hydroxydecanoic acid) exhibited 3.3 and 6.3 fold higher IC50 values compared to R10 conjugated peptide indicating a role for the position of the hydroxyl moiety in enhancement of anti-cancer activity. Conjugation of decanoic acid (C10) to peptides resulted in similar or higher IC50 values compared to R10 conjugates but C10 conjugates did not exhibit any cancer selectivity. Combination studies showed that R10DP18L exhibited synergy with cisplatin, gemcitabine, and taxotere with IC50 values in the nanomolar range.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Biomaterials",
title = "The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate",
pages = "2718-2710",
number = "11",
volume = "34",
doi = "10.1016/j.biomaterials.2012.12.032"
}

O'Connor, S., Szwej, E., Nikodinović-Runić, J., O'Connor, A., Byrne, A. T., Devocelle, M., O'Donovan, N., Gallagher, W. M., Babu, R., Kenny, S. T., Zinn, M., Zulian, Q. R.,& O'Connor, K.. (2013). The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. in Biomaterials
Elsevier Sci Ltd, Oxford., 34(11), 2710-2718.
https://doi.org/10.1016/j.biomaterials.2012.12.032

O'Connor S, Szwej E, Nikodinović-Runić J, O'Connor A, Byrne AT, Devocelle M, O'Donovan N, Gallagher WM, Babu R, Kenny ST, Zinn M, Zulian QR, O'Connor K. The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate. in Biomaterials. 2013;34(11):2710-2718.
doi:10.1016/j.biomaterials.2012.12.032 .

O'Connor, Stephen, Szwej, Emilia, Nikodinović-Runić, Jasmina, O'Connor, Aisling, Byrne, Annette T., Devocelle, Marc, O'Donovan, Norma, Gallagher, William M., Babu, Ramesh, Kenny, Shane T., Zinn, Manfred, Zulian, Qun Ren, O'Connor, Kevin , "The anti-cancer activity of a cationic anti-microbial peptide derived from monomers of polyhydroxyalkanoate" in Biomaterials, 34, no. 11 (2013):2710-2718,
https://doi.org/10.1016/j.biomaterials.2012.12.032 . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Guzik, Maciej
AU  - Kenny, Shane T.
AU  - Babu, Ramesh
AU  - Werker, Alan
AU  - Connor, Kevin E. O.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/650
AB  - Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.
PB  - Elsevier Academic Press Inc, San Diego
T2  - Advances in Applied Microbiology, Vol 84
T1  - Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria
EP  - 200
SP  - 139
VL  - 84
DO  - 10.1016/B978-0-12-407673-0.00004-7
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Guzik, Maciej and Kenny, Shane T. and Babu, Ramesh and Werker, Alan and Connor, Kevin E. O.",
year = "2013",
abstract = "Research into the production of biodegradable polymers has been driven by vision for the most part from changes in policy, in Europe and America. These policies have their origins in the Brundtland Report of 1987, which provides a platform for a more sustainable society. Biodegradable polymers are part of the emerging portfolio of renewable raw materials seeking to deliver environmental, social, and economic benefits. Polyhydroxyalkanoates (PHAs) are naturally-occurring biodegradable-polyesters accumulated by bacteria usually in response to inorganic nutrient limitation in the presence of excess carbon. Most of the early research into PHA accumulation and technology development for industrial-scale production was undertaken using virgin starting materials. For example, polyhydroxybutyrate and copolymers such as polyhydroxybutyrate-co-valerate are produced today at industrial scale from corn-derived glucose. However, in recent years, research has been undertaken to convert domestic and industrial wastes to PHA. These wastes in today's context are residuals seen by a growing body of stakeholders as platform resources for a biobased society. In the present review, we consider residuals from food, plastic, forest and lignocellulosic, and biodiesel manufacturing (glycerol). Thus, this review seeks to gain perspective of opportunities from literature reporting the production of PHA from carbon-rich residuals as feedstocks. A discussion on approaches and context for PHA production with reference to pure- and mixed-culture technologies is provided. Literature reports advocate results of the promise of waste conversion to PHA. However, the vast majority of studies on waste to PHA is at laboratory scale. The questions of surmounting the technical and political hurdles to industrialization are generally left unanswered. There are a limited number of studies that have progressed into fermentors and a dearth of pilot-scale demonstration. A number of fermentation studies show that biomass and PHA productivity can be increased, and sometimes dramatically, in a fermentor. The relevant application-specific properties of the polymers from the wastes studied and the effect of altered-waste composition on polymer properties are generally not well reported and would greatly benefit the progress of the research as high productivity is of limited value without the context of requisite case-specific polymer properties. The proposed use of a waste residual is advantageous from a life cycle viewpoint as it removes the direct or indirect effect of PHA production on land usage and food production. However, the question, of how economic drivers will promote or hinder advancements to demonstration scale, when wastes generally become understood as resources for a biobased society, hangs today in the balance due to a lack of shared vision and the legacy of mistakes made with first generation bioproducts.",
publisher = "Elsevier Academic Press Inc, San Diego",
journal = "Advances in Applied Microbiology, Vol 84",
title = "Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria",
pages = "200-139",
volume = "84",
doi = "10.1016/B978-0-12-407673-0.00004-7"
}

Nikodinović-Runić, J., Guzik, M., Kenny, S. T., Babu, R., Werker, A.,& Connor, K. E. O.. (2013). Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria. in Advances in Applied Microbiology, Vol 84
Elsevier Academic Press Inc, San Diego., 84, 139-200.
https://doi.org/10.1016/B978-0-12-407673-0.00004-7

Nikodinović-Runić J, Guzik M, Kenny ST, Babu R, Werker A, Connor KEO. Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria. in Advances in Applied Microbiology, Vol 84. 2013;84:139-200.
doi:10.1016/B978-0-12-407673-0.00004-7 .

Nikodinović-Runić, Jasmina, Guzik, Maciej, Kenny, Shane T., Babu, Ramesh, Werker, Alan, Connor, Kevin E. O., "Carbon-Rich Wastes as Feedstocks for Biodegradable Polymer (Polyhydroxyalkanoate) Production Using Bacteria" in Advances in Applied Microbiology, Vol 84, 84 (2013):139-200,
https://doi.org/10.1016/B978-0-12-407673-0.00004-7 . .

TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh
AU  - O'Connor, Kevin 
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/563
AB  - Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate
EP  - 633
IS  - 3
SP  - 623
VL  - 95
DO  - 10.1007/s00253-012-4058-4
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh and O'Connor, Kevin ",
year = "2012",
abstract = "Sodium terephthalate (TA) produced from a PET pyrolysis product and waste glycerol (WG) from biodiesel manufacture were supplied to Pseudomonas putida GO16 in a fed-batch bioreactor. Six feeding strategies were employed by altering the sequence of TA and WG feeding. P. putida GO16 reached 8.70 g/l cell dry weight (CDW) and 2.61 g/l PHA in 48 h when grown on TA alone. When TA and WG were supplied in combination, biomass productivity (g/l/h) was increased between 1.3- and 1.7-fold and PHA productivity (g/l/h) was increased 1.8- to 2.2-fold compared to TA supplied alone. The monomer composition of the PHA accumulated from TA or WG was predominantly composed of 3-hydroxydecanoic acid. PHA monomers 3-hydroxytetradeeanoic acid and 3-hydroxytetradecenoic acid were not present in PHA accumulated from TA alone but were present when WG was supplied to the fermentation. When WG was either the sole carbon source or the predominant carbon source supplied to the fermentation the molecular weight of PHA accumulated was lower compared to PHA accumulated when TA was supplied as the sole substrate. Despite similarities in data for the properties of the polymers, PHAs produced with WG present in the PHA accumulation phase were tacky while PHA produced where TA was the sole carbon substrate in the polymer accumulation phase exhibited little or no tackiness at room temperature. The co-feeding of WG to fermentations allows for increased utilisation of TA. The order of feeding of WG and TA has an effect on TA utilisation and polymer properties.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate",
pages = "633-623",
number = "3",
volume = "95",
doi = "10.1007/s00253-012-4058-4"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R.,& O'Connor, K.. (2012). Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 95(3), 623-633.
https://doi.org/10.1007/s00253-012-4058-4

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu R, O'Connor K. Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2012;95(3):623-633.
doi:10.1007/s00253-012-4058-4 .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh, O'Connor, Kevin , "Development of a bioprocess to convert PET derived terephthalic acid and biodiesel derived glycerol to medium chain length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 95, no. 3 (2012):623-633,
https://doi.org/10.1007/s00253-012-4058-4 . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Martin, Leona B.
AU  - Babu, Ramesh
AU  - Blau, Werner
AU  - O'Connor, Kevin 
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/353
AB  - Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates  gt  30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H2O2) exhibiting 1.5 times smaller zones of inhibition at 10 mM H2O2 compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.
PB  - Oxford Univ Press, Oxford
T2  - FEMS Microbiology Letters
T1  - Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6
EP  - 183
IS  - 2
SP  - 174
VL  - 298
DO  - 10.1111/j.1574-6968.2009.01716.x
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Martin, Leona B. and Babu, Ramesh and Blau, Werner and O'Connor, Kevin ",
year = "2009",
abstract = "Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates  gt  30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H2O2) exhibiting 1.5 times smaller zones of inhibition at 10 mM H2O2 compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.",
publisher = "Oxford Univ Press, Oxford",
journal = "FEMS Microbiology Letters",
title = "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6",
pages = "183-174",
number = "2",
volume = "298",
doi = "10.1111/j.1574-6968.2009.01716.x"
}

Nikodinović-Runić, J., Martin, L. B., Babu, R., Blau, W.,& O'Connor, K.. (2009). Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters
Oxford Univ Press, Oxford., 298(2), 174-183.
https://doi.org/10.1111/j.1574-6968.2009.01716.x

Nikodinović-Runić J, Martin LB, Babu R, Blau W, O'Connor K. Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. in FEMS Microbiology Letters. 2009;298(2):174-183.
doi:10.1111/j.1574-6968.2009.01716.x .

Nikodinović-Runić, Jasmina, Martin, Leona B., Babu, Ramesh, Blau, Werner, O'Connor, Kevin , "Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6" in FEMS Microbiology Letters, 298, no. 2 (2009):174-183,
https://doi.org/10.1111/j.1574-6968.2009.01716.x . .

TY  - JOUR
AU  - Kenny, Shane T.
AU  - Nikodinović-Runić, Jasmina
AU  - Kaminsky, Walter
AU  - Woods, Trevor
AU  - Babu, Ramesh
AU  - Keely, Chris M.
AU  - Blau, Werner
AU  - O'Connor, Kevin 
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/323
AB  - The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.
PB  - Amer Chemical Soc, Washington
T2  - Environmental Science & Technology
T1  - Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)
EP  - 7701
IS  - 20
SP  - 7696
VL  - 42
DO  - 10.1021/es801010e
ER  - 

@article{
author = "Kenny, Shane T. and Nikodinović-Runić, Jasmina and Kaminsky, Walter and Woods, Trevor and Babu, Ramesh and Keely, Chris M. and Blau, Werner and O'Connor, Kevin ",
year = "2008",
abstract = "The conversion of the petrochemical polymer polyethylene terephthalate (PET) to a biodegradable plastic polyhydroxyalkanoate (PHA) is described here. PET was pyrolised at 450 degrees C resulting in the production of a solid, liquid, and gaseous fraction. The liquid and gaseous fractions were burnt for energy recovery, whereas the solid fraction terephthalic acid (TA) was used as the feedstock for bacterial production of PHA. Strains previously reported to grow on TA were unable to accumulate PHA. We therefore isolated bacteria from soil exposed to PET granules at a PET bottle processing plant. From the 32 strains isolated, three strains capable of accumulation of medium chain length PHA (mclPHA) from TA as a sole source of carbon and energy were selected for further study. These isolates were identified using 16S rDNA techniques as P. putida (GO16), P. putida (GO19), and P. frederiksbergensis (GO23). P. putida GO16 and GO19 accumulate PHA composed predominantly of a 3-hydroxydecanoic acid monomer while P. frederiksbergensis GO23 accumulates 3-hydroxydecanoic acid as the predominant monomer with increased amounts of 3-hydroxydodecanoic acid and 3-hydroxydodecenoic acid compared to the other two strains. PHA was detected in all three strains when nitrogen depleted below detectable levels in the growth medium. Strains GO16 and GO19 accumulate PHA at a maximal rate of approximately 8.4 mg PHA/l/h for 12 h before the rate of PHA accumulation decreased dramatically. Strain GO23 accumulates PHA at a lower maximal rate of 4.4 mg PHA/l/h but there was no slow down in the rate of PHA accumulation over time. Each of the PHA polymers is a thermoplastic with the onset of thermal degradation occurring around 308 degrees C with the complete degradation occurring by 370 degrees C. The molecular weight ranged from 74 to 123 kDa. X-ray diffraction indicated crystallinity of the order of 18-31%. Thermal analysis shows a low glass transition (-53 degrees C) with a broad melting endotherm between 0 and 45 degrees C.",
publisher = "Amer Chemical Soc, Washington",
journal = "Environmental Science & Technology",
title = "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)",
pages = "7701-7696",
number = "20",
volume = "42",
doi = "10.1021/es801010e"
}

Kenny, S. T., Nikodinović-Runić, J., Kaminsky, W., Woods, T., Babu, R., Keely, C. M., Blau, W.,& O'Connor, K.. (2008). Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science & Technology
Amer Chemical Soc, Washington., 42(20), 7696-7701.
https://doi.org/10.1021/es801010e

Kenny ST, Nikodinović-Runić J, Kaminsky W, Woods T, Babu R, Keely CM, Blau W, O'Connor K. Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate). in Environmental Science & Technology. 2008;42(20):7696-7701.
doi:10.1021/es801010e .

Kenny, Shane T., Nikodinović-Runić, Jasmina, Kaminsky, Walter, Woods, Trevor, Babu, Ramesh, Keely, Chris M., Blau, Werner, O'Connor, Kevin , "Up-Cycling of PET (Polyethylene Terephthalate) to the Biodegradable Plastic PHA (Polyhydroxyalkanoate)" in Environmental Science & Technology, 42, no. 20 (2008):7696-7701,
https://doi.org/10.1021/es801010e . .

TY  - JOUR
AU  - Nikodinović-Runić, Jasmina
AU  - Kenny, Shane T.
AU  - Babu, Ramesh
AU  - Woods, Trevor
AU  - Blau, Werner J.
AU  - O'Connor, Kevin 
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/327
AB  - Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.
PB  - Springer, New York
T2  - Applied Microbiology and Biotechnology
T1  - The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate
EP  - 673
IS  - 4
SP  - 665
VL  - 80
DO  - 10.1007/s00253-008-1593-0
ER  - 

@article{
author = "Nikodinović-Runić, Jasmina and Kenny, Shane T. and Babu, Ramesh and Woods, Trevor and Blau, Werner J. and O'Connor, Kevin ",
year = "2008",
abstract = "Here, we report the use of petrochemical aromatic hydrocarbons as a feedstock for the biotechnological conversion into valuable biodegradable plastic polymers-polyhydroxyalkanoates (PHAs). We assessed the ability of the known Pseudomonas putida species that are able to utilize benzene, toluene, ethylbenzene, p-xylene (BTEX) compounds as a sole carbon and energy source for their ability to produce PHA from the single substrates. P. putida F1 is able to accumulate medium-chain-length (mcl) PHA when supplied with toluene, benzene, or ethylbenzene. P. putida mt-2 accumulates mcl-PHA when supplied with toluene or p-xylene. The highest level of PHA accumulated by cultures in shake flask was 26% cell dry weight for P. putida mt-2 supplied with p-xylene. A synthetic mixture of benzene, toluene, ethylbenzene, p-xylene, and styrene (BTEXS) which mimics the aromatic fraction of mixed plastic pyrolysis oil was supplied to a defined mixed culture of P. putida F1, mt-2, and CA-3 in the shake flasks and fermentation experiments. PHA was accumulated to 24% and to 36% of the cell dry weight of the shake flask and fermentation grown cultures respectively. In addition a three-fold higher cell density was achieved with the mixed culture grown in the bioreactor compared to shake flask experiments. A run in the 5-l fermentor resulted in the utilization of 59.6 g (67.5 ml) of the BTEXS mixture and the production of 6 g of mcl-PHA. The monomer composition of PHA accumulated by the mixed culture was the same as that accumulated by single strains supplied with single substrates with 3-hydroxydecanoic acid occurring as the predominant monomer. The purified polymer was partially crystalline with an average molecular weight of 86.9 kDa. It has a thermal degradation temperature of 350 degrees C and a glass transition temperature of -48.5 degrees C.",
publisher = "Springer, New York",
journal = "Applied Microbiology and Biotechnology",
title = "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate",
pages = "673-665",
number = "4",
volume = "80",
doi = "10.1007/s00253-008-1593-0"
}

Nikodinović-Runić, J., Kenny, S. T., Babu, R., Woods, T., Blau, W. J.,& O'Connor, K.. (2008). The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology
Springer, New York., 80(4), 665-673.
https://doi.org/10.1007/s00253-008-1593-0

Nikodinović-Runić J, Kenny ST, Babu R, Woods T, Blau WJ, O'Connor K. The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate. in Applied Microbiology and Biotechnology. 2008;80(4):665-673.
doi:10.1007/s00253-008-1593-0 .

Nikodinović-Runić, Jasmina, Kenny, Shane T., Babu, Ramesh, Woods, Trevor, Blau, Werner J., O'Connor, Kevin , "The conversion of BTEX compounds by single and defined mixed cultures to medium-chain-length polyhydroxyalkanoate" in Applied Microbiology and Biotechnology, 80, no. 4 (2008):665-673,
https://doi.org/10.1007/s00253-008-1593-0 . .