TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 

@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}

Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364

Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

TY  - CONF
AU  - Bojić, Luka
AU  - Pejić, Jelena
AU  - Stojkovska, Jasmina
AU  - Stevanović, Milena
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Milivojević, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1262
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2366
AB  - Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.
C3  - Hemijska industrija (Chemical Industry)
T1  - Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems
EP  - 20
IS  - 1S
SP  - 20
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2366
ER  - 

@conference{
author = "Bojić, Luka and Pejić, Jelena and Stojkovska, Jasmina and Stevanović, Milena and Medić, Aleksandra and Petrović, Isidora and Milivojević, Milena",
year = "2024",
abstract = "Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems",
pages = "20-20",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2366"
}

Bojić, L., Pejić, J., Stojkovska, J., Stevanović, M., Medić, A., Petrović, I.,& Milivojević, M.. (2024). Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry), 78(1S), 20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366

Bojić L, Pejić J, Stojkovska J, Stevanović M, Medić A, Petrović I, Milivojević M. Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry). 2024;78(1S):20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

Bojić, Luka, Pejić, Jelena, Stojkovska, Jasmina, Stevanović, Milena, Medić, Aleksandra, Petrović, Isidora, Milivojević, Milena, "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):20-20,
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

TY  - CONF
AU  - Bojić, Luka
AU  - Schwirtlich, Marija
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Balint, Vanda
AU  - Stevanović, Milena
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2133
AB  - Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro
EP  - 143
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2133
ER  - 

@conference{
author = "Bojić, Luka and Schwirtlich, Marija and Lazić, Stefan and Stanisavljević Ninković, Danijela and Balint, Vanda and Stevanović, Milena and Milivojević, Milena",
year = "2023",
abstract = "Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2133"
}

Bojić, L., Schwirtlich, M., Lazić, S., Stanisavljević Ninković, D., Balint, V., Stevanović, M.,& Milivojević, M.. (2023). The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2133

Bojić L, Schwirtlich M, Lazić S, Stanisavljević Ninković D, Balint V, Stevanović M, Milivojević M. The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2133 .

Bojić, Luka, Schwirtlich, Marija, Lazić, Stefan, Stanisavljević Ninković, Danijela, Balint, Vanda, Stevanović, Milena, Milivojević, Milena, "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):143-143,
https://hdl.handle.net/21.15107/rcub_imagine_2133 .

TY  - CONF
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Aleksandra, Medić
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Erceg, Slaven
AU  - Stevanović, Milena
AU  - Švirtlih, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2287
AB  - Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2287
ER  - 

@conference{
author = "Lazić, Stefan and Stanisavljević Ninković, Danijela and Petrović, Isidora and Aleksandra, Medić and Milivojević, Milena and Bojić, Luka and Erceg, Slaven and Stevanović, Milena and Švirtlih, Marija",
year = "2023",
abstract = "Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2287"
}

Lazić, S., Stanisavljević Ninković, D., Petrović, I., Aleksandra, M., Milivojević, M., Bojić, L., Erceg, S., Stevanović, M.,& Švirtlih, M.. (2023). Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287

Lazić S, Stanisavljević Ninković D, Petrović I, Aleksandra M, Milivojević M, Bojić L, Erceg S, Stevanović M, Švirtlih M. Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society. 2023;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

Lazić, Stefan, Stanisavljević Ninković, Danijela, Petrović, Isidora, Aleksandra, Medić, Milivojević, Milena, Bojić, Luka, Erceg, Slaven, Stevanović, Milena, Švirtlih, Marija, "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21" in 8th Congress of the Serbian Neuroscience Society (2023):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2287 .