TY  - CONF
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2181
AB  - Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.
PB  - Springer Nature
C3  - European Journal of Human Genetics
T1  - Detection rate of 22q11.2 microdeletion using strict diagnostic criteria
EP  - 240
IS  - Suppl 1
SP  - 240
VL  - 31
DO  - 10.1038/s41431-023-01339-3
ER  - 

@conference{
author = "Drakulić, Danijela and Cuturilo, Goran and Jovanović, Ida and Krstić, Aleksandar and Milivojević, Milena and Stevanović, Milena",
year = "2023",
abstract = "Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.",
publisher = "Springer Nature",
journal = "European Journal of Human Genetics",
title = "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria",
pages = "240-240",
number = "Suppl 1",
volume = "31",
doi = "10.1038/s41431-023-01339-3"
}

Drakulić, D., Cuturilo, G., Jovanović, I., Krstić, A., Milivojević, M.,& Stevanović, M.. (2023). Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics
Springer Nature., 31(Suppl 1), 240-240.
https://doi.org/10.1038/s41431-023-01339-3

Drakulić D, Cuturilo G, Jovanović I, Krstić A, Milivojević M, Stevanović M. Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics. 2023;31(Suppl 1):240-240.
doi:10.1038/s41431-023-01339-3 .

Drakulić, Danijela, Cuturilo, Goran, Jovanović, Ida, Krstić, Aleksandar, Milivojević, Milena, Stevanović, Milena, "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria" in European Journal of Human Genetics, 31, no. Suppl 1 (2023):240-240,
https://doi.org/10.1038/s41431-023-01339-3 . .

TY  - JOUR
AU  - Topalović, Vladanka
AU  - Krstić, Aleksandar
AU  - Schwirtlich, Marija
AU  - Dolfini, Diletta
AU  - Mantovani, Roberto
AU  - Stevanović, Milena
AU  - Mojsin, Marija
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1005
AB  - Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells
IS  - 9
VL  - 12
DO  - 10.1371/journal.pone.0184099
ER  - 

@article{
author = "Topalović, Vladanka and Krstić, Aleksandar and Schwirtlich, Marija and Dolfini, Diletta and Mantovani, Roberto and Stevanović, Milena and Mojsin, Marija",
year = "2017",
abstract = "Sox3/SOX3 is one of the earliest neural markers in vertebrates. Together with the Sox1/SOX1 and Sox2/SOX2 genes it is implicated in the regulation of stem cell identity. In the present study, we performed the first analysis of epigenetic mechanisms (DNA methylation and histone marks) involved in the regulation of the human SOX3 gene expression during RA-induced neural differentiation of NT2/D1 cells. We show that the promoter of the human SOX3 gene is extremely hypomethylated both in undifferentiated NT2/D1 cells and during the early phases of RA-induced neural differentiation. By employing chromatin immunopre-cipitation, we analyze several histone modifications across different regions of the SOX3 gene and their dynamics following initiation of differentiation. In the same timeframe we investigate profiles of selected histone marks on the promoters of human SOX1 and SOX2 genes. We demonstrate differences in histone signatures of SOX1, SOX2 and SOX3 genes. Considering the importance of SOXB1 genes in the process of neural differentiation, the present study contributes to a better understanding of epigenetic mechanisms implicated in the regulation of pluripotency maintenance and commitment towards the neural lineage.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells",
number = "9",
volume = "12",
doi = "10.1371/journal.pone.0184099"
}

Topalović, V., Krstić, A., Schwirtlich, M., Dolfini, D., Mantovani, R., Stevanović, M.,& Mojsin, M.. (2017). Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. in PLoS One
Public Library Science, San Francisco., 12(9).
https://doi.org/10.1371/journal.pone.0184099

Topalović V, Krstić A, Schwirtlich M, Dolfini D, Mantovani R, Stevanović M, Mojsin M. Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. in PLoS One. 2017;12(9).
doi:10.1371/journal.pone.0184099 .

Topalović, Vladanka, Krstić, Aleksandar, Schwirtlich, Marija, Dolfini, Diletta, Mantovani, Roberto, Stevanović, Milena, Mojsin, Marija, "Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells" in PLoS One, 12, no. 9 (2017),
https://doi.org/10.1371/journal.pone.0184099 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Đukić, Milan
AU  - Skorić, Dejan
AU  - Mijović, Marija
AU  - Stefanović, Igor
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/961
AB  - Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries.
PB  - Springer India
T2  - Indian Pediatrics
T1  - Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia
EP  - 789
IS  - 9
SP  - 786
VL  - 53
DO  - 10.1007/s13312-016-0931-z
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Jovanović, Ida and Krstić, Aleksandar and Đukić, Milan and Skorić, Dejan and Mijović, Marija and Stefanović, Igor and Milivojević, Milena and Stevanović, Milena",
year = "2016",
abstract = "Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries.",
publisher = "Springer India",
journal = "Indian Pediatrics",
title = "Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia",
pages = "789-786",
number = "9",
volume = "53",
doi = "10.1007/s13312-016-0931-z"
}

Cuturilo, G., Drakulić, D., Jovanović, I., Krstić, A., Đukić, M., Skorić, D., Mijović, M., Stefanović, I., Milivojević, M.,& Stevanović, M.. (2016). Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia. in Indian Pediatrics
Springer India., 53(9), 786-789.
https://doi.org/10.1007/s13312-016-0931-z

Cuturilo G, Drakulić D, Jovanović I, Krstić A, Đukić M, Skorić D, Mijović M, Stefanović I, Milivojević M, Stevanović M. Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia. in Indian Pediatrics. 2016;53(9):786-789.
doi:10.1007/s13312-016-0931-z .

Cuturilo, Goran, Drakulić, Danijela, Jovanović, Ida, Krstić, Aleksandar, Đukić, Milan, Skorić, Dejan, Mijović, Marija, Stefanović, Igor, Milivojević, Milena, Stevanović, Milena, "Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia" in Indian Pediatrics, 53, no. 9 (2016):786-789,
https://doi.org/10.1007/s13312-016-0931-z . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Vicentić, Jelena Marjanovic
AU  - Schwirtlich, Marija
AU  - Tosić, Jelena
AU  - Krstić, Aleksandar
AU  - Lazić, Andrijana
AU  - Stevanović, Milena
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/889
AB  - The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis.
PB  - Acad Brasileira De Ciencias, Rio Janeiro
T2  - Anais da Academia Brasileira de Ciencias
T1  - The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1
EP  - 405
IS  - 1
SP  - 389
VL  - 87
DO  - 10.1590/0001-3765201520140352
ER  - 

@article{
author = "Drakulić, Danijela and Vicentić, Jelena Marjanovic and Schwirtlich, Marija and Tosić, Jelena and Krstić, Aleksandar and Lazić, Andrijana and Stevanović, Milena",
year = "2015",
abstract = "The altered expression of the SOX2 transcription factor is associated with oncogenic or tumor suppressor functions in human cancers. This factor regulates the migration and invasion of different cancer cells. In this study we investigated the effect of constitutive SOX2 overexpression on the migration and adhesion capacity of embryonal teratocarcinoma NT2/D1 cells derived from a metastasis of a human testicular germ cell tumor. We detected that increased SOX2 expression changed the speed, mode and path of cell migration, but not the adhesion ability of NT2/D1 cells. Additionally, we demonstrated that SOX2 overexpression increased the expression of the tumor suppressor protein p53 and the HDM2 oncogene. Our results contribute to the better understanding of the effect of SOX2 on the behavior of tumor cells originating from a human testicular germ cell tumor. Considering that NT2/D1 cells resemble cancer stem cells in many features, our results could contribute to the elucidation of the role of SOX2 in cancer stem cells behavior and the process of metastasis.",
publisher = "Acad Brasileira De Ciencias, Rio Janeiro",
journal = "Anais da Academia Brasileira de Ciencias",
title = "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1",
pages = "405-389",
number = "1",
volume = "87",
doi = "10.1590/0001-3765201520140352"
}

Drakulić, D., Vicentić, J. M., Schwirtlich, M., Tosić, J., Krstić, A., Lazić, A.,& Stevanović, M.. (2015). The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1. in Anais da Academia Brasileira de Ciencias
Acad Brasileira De Ciencias, Rio Janeiro., 87(1), 389-405.
https://doi.org/10.1590/0001-3765201520140352

Drakulić D, Vicentić JM, Schwirtlich M, Tosić J, Krstić A, Lazić A, Stevanović M. The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1. in Anais da Academia Brasileira de Ciencias. 2015;87(1):389-405.
doi:10.1590/0001-3765201520140352 .

Drakulić, Danijela, Vicentić, Jelena Marjanovic, Schwirtlich, Marija, Tosić, Jelena, Krstić, Aleksandar, Lazić, Andrijana, Stevanović, Milena, "The overexpression of SOX2 affects the migration of human teratocarcinoma cell line NT2/D1" in Anais da Academia Brasileira de Ciencias, 87, no. 1 (2015):389-405,
https://doi.org/10.1590/0001-3765201520140352 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Krstić, Aleksandar
AU  - Gradinac, Marija
AU  - Ilisić, Tamara
AU  - Parezanović, Vojislav
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Jovanović, Ida
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/678
AB  - Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients.
PB  - Cambridge Univ Press, Cambridge
T2  - Cardiology in the Young
T1  - The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2
EP  - 188
IS  - 2
SP  - 181
VL  - 23
DO  - 10.1017/S1047951112000571
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Krstić, Aleksandar and Gradinac, Marija and Ilisić, Tamara and Parezanović, Vojislav and Milivojević, Milena and Stevanović, Milena and Jovanović, Ida",
year = "2013",
abstract = "Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Cardiology in the Young",
title = "The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2",
pages = "188-181",
number = "2",
volume = "23",
doi = "10.1017/S1047951112000571"
}

Cuturilo, G., Drakulić, D., Krstić, A., Gradinac, M., Ilisić, T., Parezanović, V., Milivojević, M., Stevanović, M.,& Jovanović, I.. (2013). The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2. in Cardiology in the Young
Cambridge Univ Press, Cambridge., 23(2), 181-188.
https://doi.org/10.1017/S1047951112000571

Cuturilo G, Drakulić D, Krstić A, Gradinac M, Ilisić T, Parezanović V, Milivojević M, Stevanović M, Jovanović I. The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2. in Cardiology in the Young. 2013;23(2):181-188.
doi:10.1017/S1047951112000571 .

Cuturilo, Goran, Drakulić, Danijela, Krstić, Aleksandar, Gradinac, Marija, Ilisić, Tamara, Parezanović, Vojislav, Milivojević, Milena, Stevanović, Milena, Jovanović, Ida, "The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2" in Cardiology in the Young, 23, no. 2 (2013):181-188,
https://doi.org/10.1017/S1047951112000571 . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Krstić, A.
AU  - Stevanović, Milena
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/561
AB  - SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and characterized SOX2-overexpressing NT2/D1 cell clones. Using Southern blot and semi-quantitative RT-PCR, we confirmed integration and expression of exogenous SOX2 in three NT2/D1 cell clones. Overexpression of the SOX2 gene was detected in two of these clones. SOX2 overexpression in NT2/D1 cell clones resulted in altered expression of key pluripotency genes OCT4 and NANOG. Furthermore, SOX2-overexpressing NT2/D1 cell clones entered into retinoic acid-dependent neural differentiation, even when there was elevated SOX2 expression. After 21 days of induction by retinoic acid, expression of neural markers (neuroD1 and synaptophysin) was higher in induced cell clones than in induced parental cells. The cell clone with SOX2 overexpression had an approximately 1.3-fold higher growth rate compared to parental cells. SOX2 overexpression did not increase the population of cells undergoing apoptosis. Taken together, we developed two SOX2-overexpressing cell clones, with constitutive SOX2 expression after three weeks of retinoic acid treatment. SOX2 overexpression resulted in altered expression of pluripotency-related genes, increased proliferation, and altered expression of neural markers after three weeks of retinoic acid treatment.
PB  - FUNPEC-Editora, Ribeirao Preto
T2  - Genetics and Molecular Research
T1  - Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones
EP  - 1400
IS  - 2
SP  - 1385
VL  - 11
DO  - 10.4238/2012.May.15.9
ER  - 

@article{
author = "Drakulić, Danijela and Krstić, A. and Stevanović, Milena",
year = "2012",
abstract = "SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and characterized SOX2-overexpressing NT2/D1 cell clones. Using Southern blot and semi-quantitative RT-PCR, we confirmed integration and expression of exogenous SOX2 in three NT2/D1 cell clones. Overexpression of the SOX2 gene was detected in two of these clones. SOX2 overexpression in NT2/D1 cell clones resulted in altered expression of key pluripotency genes OCT4 and NANOG. Furthermore, SOX2-overexpressing NT2/D1 cell clones entered into retinoic acid-dependent neural differentiation, even when there was elevated SOX2 expression. After 21 days of induction by retinoic acid, expression of neural markers (neuroD1 and synaptophysin) was higher in induced cell clones than in induced parental cells. The cell clone with SOX2 overexpression had an approximately 1.3-fold higher growth rate compared to parental cells. SOX2 overexpression did not increase the population of cells undergoing apoptosis. Taken together, we developed two SOX2-overexpressing cell clones, with constitutive SOX2 expression after three weeks of retinoic acid treatment. SOX2 overexpression resulted in altered expression of pluripotency-related genes, increased proliferation, and altered expression of neural markers after three weeks of retinoic acid treatment.",
publisher = "FUNPEC-Editora, Ribeirao Preto",
journal = "Genetics and Molecular Research",
title = "Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones",
pages = "1400-1385",
number = "2",
volume = "11",
doi = "10.4238/2012.May.15.9"
}

Drakulić, D., Krstić, A.,& Stevanović, M.. (2012). Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones. in Genetics and Molecular Research
FUNPEC-Editora, Ribeirao Preto., 11(2), 1385-1400.
https://doi.org/10.4238/2012.May.15.9

Drakulić D, Krstić A, Stevanović M. Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones. in Genetics and Molecular Research. 2012;11(2):1385-1400.
doi:10.4238/2012.May.15.9 .

Drakulić, Danijela, Krstić, A., Stevanović, Milena, "Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones" in Genetics and Molecular Research, 11, no. 2 (2012):1385-1400,
https://doi.org/10.4238/2012.May.15.9 . .

TY  - JOUR
AU  - Nikčević, Gordana
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Krstić, A.
AU  - Savić, T.
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/485
AB  - Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates. Despite the mounting evidence that Sox3/SOX3 is one of the key players in the development of the nervous system, limited data are available regarding the transcriptional regulation of its expression. This review is focused on the retinoic acid induced regulation of SOX3 gene expression, with particular emphasis on the involvement of retinoid receptors. Experiments with human embryonal carcinoma cells identified two response elements involved in retinoic acid/retinoid X receptor-dependent activation of the SOX3 gene expression: distal atypical retinoic acid-response element, consisting of two unique G-rich boxes separated by 49 bp, and proximal element comprising DR-3-like motif, composed of two imperfect hexameric half-sites. Importantly, the retinoic acid-induced SOX3 gene expression could be significantly down-regulated by a synthetic antagonist of retinoid receptors. This cell model provides a solid base for further studies on mechanism(s) underlying regulation of expression of SOX3 gene, which could improve the understanding of molecular signals that induce neurogenesis in the stem/progenitor cells both during development and in adulthood.
PB  - Acad Sciences Czech Republic, Inst Physiology, Prague 4
T2  - Physiological Research
T1  - Regulation of the SOX3 Gene Expression by Retinoid Receptors
EP  - S91
SP  - S83
VL  - 60
DO  - 10.33549/physiolres.932184
ER  - 

@article{
author = "Nikčević, Gordana and Kovačević Grujičić, Nataša and Mojsin, Marija and Krstić, A. and Savić, T. and Stevanović, Milena",
year = "2011",
abstract = "Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates. Despite the mounting evidence that Sox3/SOX3 is one of the key players in the development of the nervous system, limited data are available regarding the transcriptional regulation of its expression. This review is focused on the retinoic acid induced regulation of SOX3 gene expression, with particular emphasis on the involvement of retinoid receptors. Experiments with human embryonal carcinoma cells identified two response elements involved in retinoic acid/retinoid X receptor-dependent activation of the SOX3 gene expression: distal atypical retinoic acid-response element, consisting of two unique G-rich boxes separated by 49 bp, and proximal element comprising DR-3-like motif, composed of two imperfect hexameric half-sites. Importantly, the retinoic acid-induced SOX3 gene expression could be significantly down-regulated by a synthetic antagonist of retinoid receptors. This cell model provides a solid base for further studies on mechanism(s) underlying regulation of expression of SOX3 gene, which could improve the understanding of molecular signals that induce neurogenesis in the stem/progenitor cells both during development and in adulthood.",
publisher = "Acad Sciences Czech Republic, Inst Physiology, Prague 4",
journal = "Physiological Research",
title = "Regulation of the SOX3 Gene Expression by Retinoid Receptors",
pages = "S91-S83",
volume = "60",
doi = "10.33549/physiolres.932184"
}

Nikčević, G., Kovačević Grujičić, N., Mojsin, M., Krstić, A., Savić, T.,& Stevanović, M.. (2011). Regulation of the SOX3 Gene Expression by Retinoid Receptors. in Physiological Research
Acad Sciences Czech Republic, Inst Physiology, Prague 4., 60, S83-S91.
https://doi.org/10.33549/physiolres.932184

Nikčević G, Kovačević Grujičić N, Mojsin M, Krstić A, Savić T, Stevanović M. Regulation of the SOX3 Gene Expression by Retinoid Receptors. in Physiological Research. 2011;60:S83-S91.
doi:10.33549/physiolres.932184 .

Nikčević, Gordana, Kovačević Grujičić, Nataša, Mojsin, Marija, Krstić, A., Savić, T., Stevanović, Milena, "Regulation of the SOX3 Gene Expression by Retinoid Receptors" in Physiological Research, 60 (2011):S83-S91,
https://doi.org/10.33549/physiolres.932184 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Menten, Bjorn
AU  - Krstić, Aleksandar
AU  - Drakulić, Danijela
AU  - Jovanović, Ida
AU  - Parezanović, Vojislav
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/494
AB  - Small terminal or interstitial deletions involving bands 4q34 and 4q35 have been described in several patients with a relatively mild phenotype such as mild to moderate intellectual disability and minor dysmorphic features. We present a boy born from unrelated parents with a de novo 4q34.1-q35.2 deletion and clinical features resembling 22q11.2 deletion syndrome. To the best of our knowledge, this is the first reported patient with 4q34-q35 deletion and phenotype resembling 22q11.2 deletion syndrome without fifth finger anomalies as a specific feature of 4q- syndrome. G-banding karyotyping disclosed the deletion, which was further delineated by microarray comparative genomic hybridization. Fluorescence in situ hybridization and multiplex ligation-dependent probe amplification analyses did not reveal rearrangements of 22q11.2 region. MLPA confirmed the deletion within the 4q35.2 region. Conclusion: Given the considerable clinical overlaps between the 22q11.2 deletion syndrome and clinical manifestation of the patient described in this study, we propose that region 4q34.1-q35.2 should be considered as another region associated with phenotype resembling 22q11.2 deletion syndrome. We also propose that distal 4q deletions should be considered in the evaluation of patients with phenotypic manifestations resembling 22q11.2 deletion syndrome in whom no 22q11.2 micro-deletion was detected, even in the absence of distinctive fifth finger anomalies. Additionally, we underline the importance of applying array CGH that enables simultaneous genome-wide detection and delineation of copy number changes (e. g., deletions and duplications).
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome
EP  - 1470
IS  - 11
SP  - 1465
VL  - 170
DO  - 10.1007/s00431-011-1533-3
ER  - 

@article{
author = "Cuturilo, Goran and Menten, Bjorn and Krstić, Aleksandar and Drakulić, Danijela and Jovanović, Ida and Parezanović, Vojislav and Stevanović, Milena",
year = "2011",
abstract = "Small terminal or interstitial deletions involving bands 4q34 and 4q35 have been described in several patients with a relatively mild phenotype such as mild to moderate intellectual disability and minor dysmorphic features. We present a boy born from unrelated parents with a de novo 4q34.1-q35.2 deletion and clinical features resembling 22q11.2 deletion syndrome. To the best of our knowledge, this is the first reported patient with 4q34-q35 deletion and phenotype resembling 22q11.2 deletion syndrome without fifth finger anomalies as a specific feature of 4q- syndrome. G-banding karyotyping disclosed the deletion, which was further delineated by microarray comparative genomic hybridization. Fluorescence in situ hybridization and multiplex ligation-dependent probe amplification analyses did not reveal rearrangements of 22q11.2 region. MLPA confirmed the deletion within the 4q35.2 region. Conclusion: Given the considerable clinical overlaps between the 22q11.2 deletion syndrome and clinical manifestation of the patient described in this study, we propose that region 4q34.1-q35.2 should be considered as another region associated with phenotype resembling 22q11.2 deletion syndrome. We also propose that distal 4q deletions should be considered in the evaluation of patients with phenotypic manifestations resembling 22q11.2 deletion syndrome in whom no 22q11.2 micro-deletion was detected, even in the absence of distinctive fifth finger anomalies. Additionally, we underline the importance of applying array CGH that enables simultaneous genome-wide detection and delineation of copy number changes (e. g., deletions and duplications).",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome",
pages = "1470-1465",
number = "11",
volume = "170",
doi = "10.1007/s00431-011-1533-3"
}

Cuturilo, G., Menten, B., Krstić, A., Drakulić, D., Jovanović, I., Parezanović, V.,& Stevanović, M.. (2011). 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome. in European Journal of Pediatrics
Springer, New York., 170(11), 1465-1470.
https://doi.org/10.1007/s00431-011-1533-3

Cuturilo G, Menten B, Krstić A, Drakulić D, Jovanović I, Parezanović V, Stevanović M. 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome. in European Journal of Pediatrics. 2011;170(11):1465-1470.
doi:10.1007/s00431-011-1533-3 .

Cuturilo, Goran, Menten, Bjorn, Krstić, Aleksandar, Drakulić, Danijela, Jovanović, Ida, Parezanović, Vojislav, Stevanović, Milena, "4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome" in European Journal of Pediatrics, 170, no. 11 (2011):1465-1470,
https://doi.org/10.1007/s00431-011-1533-3 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Krstić, Aleksandar
AU  - Popović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/407
AB  - To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.
PB  - Springer/Plenum Publishers, New York
T2  - Biochemical Genetics
T1  - Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution
EP  - 623
IS  - 7-8
SP  - 612
VL  - 48
DO  - 10.1007/s10528-010-9343-2
ER  - 

@article{
author = "Mojsin, Marija and Kovačević Grujičić, Nataša and Krstić, Aleksandar and Popović, Jelena and Milivojević, Milena and Stevanović, Milena",
year = "2010",
abstract = "To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Biochemical Genetics",
title = "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution",
pages = "623-612",
number = "7-8",
volume = "48",
doi = "10.1007/s10528-010-9343-2"
}

Mojsin, M., Kovačević Grujičić, N., Krstić, A., Popović, J., Milivojević, M.,& Stevanović, M.. (2010). Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics
Springer/Plenum Publishers, New York., 48(7-8), 612-623.
https://doi.org/10.1007/s10528-010-9343-2

Mojsin M, Kovačević Grujičić N, Krstić A, Popović J, Milivojević M, Stevanović M. Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics. 2010;48(7-8):612-623.
doi:10.1007/s10528-010-9343-2 .

Mojsin, Marija, Kovačević Grujičić, Nataša, Krstić, Aleksandar, Popović, Jelena, Milivojević, Milena, Stevanović, Milena, "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution" in Biochemical Genetics, 48, no. 7-8 (2010):612-623,
https://doi.org/10.1007/s10528-010-9343-2 . .

TY  - JOUR
AU  - Krstić, Aleksandar
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2007
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/276
AB  - The presented results demonstrate that human SOX3 promoter possesses three CCAAT box control elements involved in the regulation of SOX3 gene expression in NT2/D1 cells. By mutational analysis we have shown that all three elements are of functional relevance for constitutive SOX3 expression. Electrophoretic mobility shift assays indicate that the active complexes at three sites involve the ubiquitously expressed CCAAT binding protein NF-Y. The involvement of NF-Y in the up-regulation of SOX3 expression in NT2/D1 cells was demonstrated in vivo by Northern and Western blot analyses. Furthermore, in co-transfection experiments we have shown that NF-Y mediates transcriptional activation of SOX3 promoter. Our data indicate that multiple CCAAT control elements are involved in the regulation of the SOX3 promoter, suggesting that NF-Y functions as a key regulator of SOX3 gene expression. Further, our results indicate that these elements can be recognized as modulators of retinoic acid induced activation of SOX3 expression.
PB  - Elsevier Science Inc, New York
T2  - Archives of Biochemistry and Biophysics
T1  - Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements
EP  - 173
IS  - 2
SP  - 163
VL  - 467
DO  - 10.1016/j.abb.2007.08.029
ER  - 

@article{
author = "Krstić, Aleksandar and Mojsin, Marija and Stevanović, Milena",
year = "2007",
abstract = "The presented results demonstrate that human SOX3 promoter possesses three CCAAT box control elements involved in the regulation of SOX3 gene expression in NT2/D1 cells. By mutational analysis we have shown that all three elements are of functional relevance for constitutive SOX3 expression. Electrophoretic mobility shift assays indicate that the active complexes at three sites involve the ubiquitously expressed CCAAT binding protein NF-Y. The involvement of NF-Y in the up-regulation of SOX3 expression in NT2/D1 cells was demonstrated in vivo by Northern and Western blot analyses. Furthermore, in co-transfection experiments we have shown that NF-Y mediates transcriptional activation of SOX3 promoter. Our data indicate that multiple CCAAT control elements are involved in the regulation of the SOX3 promoter, suggesting that NF-Y functions as a key regulator of SOX3 gene expression. Further, our results indicate that these elements can be recognized as modulators of retinoic acid induced activation of SOX3 expression.",
publisher = "Elsevier Science Inc, New York",
journal = "Archives of Biochemistry and Biophysics",
title = "Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements",
pages = "173-163",
number = "2",
volume = "467",
doi = "10.1016/j.abb.2007.08.029"
}

Krstić, A., Mojsin, M.,& Stevanović, M.. (2007). Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements. in Archives of Biochemistry and Biophysics
Elsevier Science Inc, New York., 467(2), 163-173.
https://doi.org/10.1016/j.abb.2007.08.029

Krstić A, Mojsin M, Stevanović M. Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements. in Archives of Biochemistry and Biophysics. 2007;467(2):163-173.
doi:10.1016/j.abb.2007.08.029 .

Krstić, Aleksandar, Mojsin, Marija, Stevanović, Milena, "Regulation of SOX3 gene expression is driven by multiple NF-Y binding elements" in Archives of Biochemistry and Biophysics, 467, no. 2 (2007):163-173,
https://doi.org/10.1016/j.abb.2007.08.029 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Nikčević, Gordana
AU  - Krstić, Aleksandar
AU  - Savić, Tijana
AU  - Stevanović, Milena
PY  - 2006
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/246
AB  - Sov3/SOX3 gene is implicated in the control of nervous system development and is considered to be one of the earliest neural markers. Expression of human SOX3 gene is modulated during the RA-induced neuronal differentiation cascade of NT2/D1 cells. Our present results demonstrate that the sequences responsible for RA-induced activation of SOX3 gene are localized within the 0.4 kb of its 5'-flanking region and implicate RXR alpha involvement in this regulation. The active RA/RXR alpha responsive region is pinned down to two regulatory elements. Only in the presence of both elements full RA/RXR alpha inducibility is achieved, suggesting they act synergistically. These elements comprise two unique G-rich boxes, separated by 49 bp, that could be considered as a novel, atypical RA-response element. Here, for the first time, we have demonstrated direct interaction of RXR alpha and SOX3 control elements. Furthermore, the functional in vivo analysis revealed that liganded RXR alpha is a potent activator of endogenous SOX3 protein expression. Since it is proven that Sox3 is critical determinant of neurogenesis our data may help in providing new insight into complex regulatory networks involved in retinoic acid induced neural differentiation of NT2/D1 cells.
PB  - Elsevier Ireland Ltd, Clare
T2  - Neuroscience Research
T1  - Mapping of the RXR alpha binding elements involved in retinoic acid induced transcriptional activation of the human SOX3 gene
EP  - 418
IS  - 4
SP  - 409
VL  - 56
DO  - 10.1016/j.neures.2006.08.010
ER  - 

@article{
author = "Mojsin, Marija and Kovačević Grujičić, Nataša and Nikčević, Gordana and Krstić, Aleksandar and Savić, Tijana and Stevanović, Milena",
year = "2006",
abstract = "Sov3/SOX3 gene is implicated in the control of nervous system development and is considered to be one of the earliest neural markers. Expression of human SOX3 gene is modulated during the RA-induced neuronal differentiation cascade of NT2/D1 cells. Our present results demonstrate that the sequences responsible for RA-induced activation of SOX3 gene are localized within the 0.4 kb of its 5'-flanking region and implicate RXR alpha involvement in this regulation. The active RA/RXR alpha responsive region is pinned down to two regulatory elements. Only in the presence of both elements full RA/RXR alpha inducibility is achieved, suggesting they act synergistically. These elements comprise two unique G-rich boxes, separated by 49 bp, that could be considered as a novel, atypical RA-response element. Here, for the first time, we have demonstrated direct interaction of RXR alpha and SOX3 control elements. Furthermore, the functional in vivo analysis revealed that liganded RXR alpha is a potent activator of endogenous SOX3 protein expression. Since it is proven that Sox3 is critical determinant of neurogenesis our data may help in providing new insight into complex regulatory networks involved in retinoic acid induced neural differentiation of NT2/D1 cells.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Neuroscience Research",
title = "Mapping of the RXR alpha binding elements involved in retinoic acid induced transcriptional activation of the human SOX3 gene",
pages = "418-409",
number = "4",
volume = "56",
doi = "10.1016/j.neures.2006.08.010"
}

Mojsin, M., Kovačević Grujičić, N., Nikčević, G., Krstić, A., Savić, T.,& Stevanović, M.. (2006). Mapping of the RXR alpha binding elements involved in retinoic acid induced transcriptional activation of the human SOX3 gene. in Neuroscience Research
Elsevier Ireland Ltd, Clare., 56(4), 409-418.
https://doi.org/10.1016/j.neures.2006.08.010

Mojsin M, Kovačević Grujičić N, Nikčević G, Krstić A, Savić T, Stevanović M. Mapping of the RXR alpha binding elements involved in retinoic acid induced transcriptional activation of the human SOX3 gene. in Neuroscience Research. 2006;56(4):409-418.
doi:10.1016/j.neures.2006.08.010 .

Mojsin, Marija, Kovačević Grujičić, Nataša, Nikčević, Gordana, Krstić, Aleksandar, Savić, Tijana, Stevanović, Milena, "Mapping of the RXR alpha binding elements involved in retinoic acid induced transcriptional activation of the human SOX3 gene" in Neuroscience Research, 56, no. 4 (2006):409-418,
https://doi.org/10.1016/j.neures.2006.08.010 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Đurović, Jelena
AU  - Petrović, Isidora
AU  - Krstić, Aleksandar
AU  - Drakulić, Danijela
AU  - Savić, Tijana
AU  - Stevanović, Milena
PY  - 2006
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/262
AB  - U ovom radu je prikazana metoda za brzu izolaciju i identifikaciju proteina koji se vezuju za specifične sekvence na DNK, zasnovana na magnetnom razdvajanju. Ovom metodom je potvrđeno direktno vezivanje humanog rekombinantnog proteina USF1 za potencijalno vezivno mesto (E blok) u promotorskom regionu humanog SOX3 gena. Pokazano je da se rekombinantni USF1 protein, u prisustvu kompetitorske DNK, specifično vezuje za DNK fragment koji je obeležen biotinom i vezan za magnetne kuglice obložene streptavidinom. Takođe, pokazano je da se protein može eluirati sa kuglica u visokom prinosu i sa očuvanim afinitetom za vezivanje za DNK. Prednost ove metode je što se, uz manje modifikacije, može uspešno primeniti za preičišćavanje i identifikaciju svih proteina koji se vezuju za specifične sekvence na DNK.
AB  - In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented. This method was applied to confirm the binding of the human recombinant USF1 protein to its putative binding site (E-box) within the human SOX3 protomer. It has been shown that biotinylated DNA attached to streptavidin magnetic particles specifically binds the USF1 protein in the presence of competitor DNA. It has also been demonstrated that the protein could be successfully eluted from the beads, in high yield and with restored DNA binding activity. The advantage of these procedures is that they could be applied for the identification and purification of any high-affinity sequence-specific DNA binding protein with only minor modifications.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Primena magnetnog razdvajanja za brzu detekciju i prečišćavanje proteina koji se vezuju za specifične sekvence na DNK
T1  - Rapid detection and purification of sequence specific DNA binding proteins using magnetic separation
EP  - 141
IS  - 2
SP  - 135
VL  - 71
DO  - 10.2298/JSC0602135M
ER  - 

@article{
author = "Mojsin, Marija and Đurović, Jelena and Petrović, Isidora and Krstić, Aleksandar and Drakulić, Danijela and Savić, Tijana and Stevanović, Milena",
year = "2006",
abstract = "U ovom radu je prikazana metoda za brzu izolaciju i identifikaciju proteina koji se vezuju za specifične sekvence na DNK, zasnovana na magnetnom razdvajanju. Ovom metodom je potvrđeno direktno vezivanje humanog rekombinantnog proteina USF1 za potencijalno vezivno mesto (E blok) u promotorskom regionu humanog SOX3 gena. Pokazano je da se rekombinantni USF1 protein, u prisustvu kompetitorske DNK, specifično vezuje za DNK fragment koji je obeležen biotinom i vezan za magnetne kuglice obložene streptavidinom. Takođe, pokazano je da se protein može eluirati sa kuglica u visokom prinosu i sa očuvanim afinitetom za vezivanje za DNK. Prednost ove metode je što se, uz manje modifikacije, može uspešno primeniti za preičišćavanje i identifikaciju svih proteina koji se vezuju za specifične sekvence na DNK., In this paper, a method for the rapid identification and purification of sequence specific DNA binding proteins based on magnetic separation is presented. This method was applied to confirm the binding of the human recombinant USF1 protein to its putative binding site (E-box) within the human SOX3 protomer. It has been shown that biotinylated DNA attached to streptavidin magnetic particles specifically binds the USF1 protein in the presence of competitor DNA. It has also been demonstrated that the protein could be successfully eluted from the beads, in high yield and with restored DNA binding activity. The advantage of these procedures is that they could be applied for the identification and purification of any high-affinity sequence-specific DNA binding protein with only minor modifications.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Primena magnetnog razdvajanja za brzu detekciju i prečišćavanje proteina koji se vezuju za specifične sekvence na DNK, Rapid detection and purification of sequence specific DNA binding proteins using magnetic separation",
pages = "141-135",
number = "2",
volume = "71",
doi = "10.2298/JSC0602135M"
}

Mojsin, M., Đurović, J., Petrović, I., Krstić, A., Drakulić, D., Savić, T.,& Stevanović, M.. (2006). Primena magnetnog razdvajanja za brzu detekciju i prečišćavanje proteina koji se vezuju za specifične sekvence na DNK. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 71(2), 135-141.
https://doi.org/10.2298/JSC0602135M

Mojsin M, Đurović J, Petrović I, Krstić A, Drakulić D, Savić T, Stevanović M. Primena magnetnog razdvajanja za brzu detekciju i prečišćavanje proteina koji se vezuju za specifične sekvence na DNK. in Journal of the Serbian Chemical Society. 2006;71(2):135-141.
doi:10.2298/JSC0602135M .

Mojsin, Marija, Đurović, Jelena, Petrović, Isidora, Krstić, Aleksandar, Drakulić, Danijela, Savić, Tijana, Stevanović, Milena, "Primena magnetnog razdvajanja za brzu detekciju i prečišćavanje proteina koji se vezuju za specifične sekvence na DNK" in Journal of the Serbian Chemical Society, 71, no. 2 (2006):135-141,
https://doi.org/10.2298/JSC0602135M . .

TY  - JOUR
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Krstić, A
AU  - Stevanović, Milena
PY  - 2005
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/238
AB  - SRY-related HMG-box genes (Sox genes) constitute a large family of developmentally regulated genes involved in the decision of cell fates during development and implicated in the control of diverse developmental processes. Sox3, an X-linked member of the family, is expressed in the central nervous system (CNS) from the earliest stages of development. It is considered to be one of the earliest neural markers in vertebrates playing the role in specifying neuronal fate. The aim of this study has been to determine and characterize the promoter of the human SOX3 gene and to elucidate molecular mechanisms underlying the regulation of its expression. In this study, we have isolated and performed the first characterization of the human SOX3 promoter. We have identified the transcription start point (tsp) and carried out the structural and functional analysis of the regulatory region responsible for SOX3 expression in NT2/D1 cell line. Using promoter reporter constructs, we have determined the minimal SOX3 promoter region that confers the basal promoter activity, as well as two regulatory elements which have positive effects on the promoter activity. We have investigated in detail the functional properties of three conserved motifs within the core promoter sequence that bind transcription factors specificity protein I (Sp1), upstream stimulatory factor (USF) and nuclear factor Y (NF-Y). By mutational analysis, we have shown that all three sites are of functional relevance for constitutive SOX3 expression in NT2/D1 cells. We have also shown that, besides the TATA motif, at least one other essential regulatory element is required for the basal transcription of the human SOX3. Taken together, data presented in this paper suggest that transcription factors such as Sp1, USF and NF-Y could function as key regulators for the basal activation of the human SOX3 gene.
PB  - Elsevier, Amsterdam
T2  - Gene
T1  - Functional characterization of the human SOX3 promoter: identification of transcription factors implicated in basal promoter activity
EP  - 297
SP  - 287
VL  - 344
DO  - 10.1016/j.gene.2004.11.006
ER  - 

@article{
author = "Kovačević Grujičić, Nataša and Mojsin, Marija and Krstić, A and Stevanović, Milena",
year = "2005",
abstract = "SRY-related HMG-box genes (Sox genes) constitute a large family of developmentally regulated genes involved in the decision of cell fates during development and implicated in the control of diverse developmental processes. Sox3, an X-linked member of the family, is expressed in the central nervous system (CNS) from the earliest stages of development. It is considered to be one of the earliest neural markers in vertebrates playing the role in specifying neuronal fate. The aim of this study has been to determine and characterize the promoter of the human SOX3 gene and to elucidate molecular mechanisms underlying the regulation of its expression. In this study, we have isolated and performed the first characterization of the human SOX3 promoter. We have identified the transcription start point (tsp) and carried out the structural and functional analysis of the regulatory region responsible for SOX3 expression in NT2/D1 cell line. Using promoter reporter constructs, we have determined the minimal SOX3 promoter region that confers the basal promoter activity, as well as two regulatory elements which have positive effects on the promoter activity. We have investigated in detail the functional properties of three conserved motifs within the core promoter sequence that bind transcription factors specificity protein I (Sp1), upstream stimulatory factor (USF) and nuclear factor Y (NF-Y). By mutational analysis, we have shown that all three sites are of functional relevance for constitutive SOX3 expression in NT2/D1 cells. We have also shown that, besides the TATA motif, at least one other essential regulatory element is required for the basal transcription of the human SOX3. Taken together, data presented in this paper suggest that transcription factors such as Sp1, USF and NF-Y could function as key regulators for the basal activation of the human SOX3 gene.",
publisher = "Elsevier, Amsterdam",
journal = "Gene",
title = "Functional characterization of the human SOX3 promoter: identification of transcription factors implicated in basal promoter activity",
pages = "297-287",
volume = "344",
doi = "10.1016/j.gene.2004.11.006"
}

Kovačević Grujičić, N., Mojsin, M., Krstić, A.,& Stevanović, M.. (2005). Functional characterization of the human SOX3 promoter: identification of transcription factors implicated in basal promoter activity. in Gene
Elsevier, Amsterdam., 344, 287-297.
https://doi.org/10.1016/j.gene.2004.11.006

Kovačević Grujičić N, Mojsin M, Krstić A, Stevanović M. Functional characterization of the human SOX3 promoter: identification of transcription factors implicated in basal promoter activity. in Gene. 2005;344:287-297.
doi:10.1016/j.gene.2004.11.006 .

Kovačević Grujičić, Nataša, Mojsin, Marija, Krstić, A, Stevanović, Milena, "Functional characterization of the human SOX3 promoter: identification of transcription factors implicated in basal promoter activity" in Gene, 344 (2005):287-297,
https://doi.org/10.1016/j.gene.2004.11.006 . .