TY  - JOUR
AU  - Novković, Mirjana
AU  - Banović Đeri, Bojana
AU  - RistivojeviĆ, Bojan
AU  - Knežević, Aleksandra
AU  - Janković, Marko
AU  - Tanasić, Vanja
AU  - Radojičić, Verica
AU  - Keckarević, Dusan
AU  - Vidanović, Dejan
AU  - Tešović, Bojana
AU  - Skakić, Anita
AU  - Tolinački, Maja
AU  - Morić, Ivana
AU  - Đorđević, Valentina
PY  - 2024
UR  - https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2024.1332276
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2327
AB  - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19-positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide.
AB  - The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19- positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide.
PB  - Frontiers
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study
VL  - 15
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2327
ER  - 

@article{
author = "Novković, Mirjana and Banović Đeri, Bojana and RistivojeviĆ, Bojan and Knežević, Aleksandra and Janković, Marko and Tanasić, Vanja and Radojičić, Verica and Keckarević, Dusan and Vidanović, Dejan and Tešović, Bojana and Skakić, Anita and Tolinački, Maja and Morić, Ivana and Đorđević, Valentina",
year = "2024",
abstract = "The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19-positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide., The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), responsible for the COVID-19 pandemic, has been evolving rapidly causing emergence of new variants and health uncertainties. Monitoring the evolution of the virus was of the utmost importance for public health interventions and the development of national and global mitigation strategies. Here, we report national data on the emergence of new variants, their distribution, and dynamics in a 3-year study conducted from March 2020 to the end of January 2023 in the Republic of Serbia. Nasopharyngeal and oropharyngeal swabs from 2,398 COVID-19- positive patients were collected and sequenced using three different next generation technologies: Oxford Nanopore, Ion Torrent, and DNBSeq. In the subset of 2,107 SARS-CoV-2 sequences which met the quality requirements, detection of mutations, assignment to SARS-CoV-2 lineages, and phylogenetic analysis were performed. During the 3-year period, we detected three variants of concern, namely, Alpha (5.6%), Delta (7.4%), and Omicron (70.3%) and one variant of interest—Omicron recombinant “Kraken” (XBB1.5) (<1%), whereas 16.8% of the samples belonged to other SARS-CoV-2 (sub)lineages. The detected SARS-CoV-2 (sub)lineages resulted in eight COVID-19 pandemic waves in Serbia, which correspond to the pandemic waves reported in Europe and the United States. Wave dynamics in Serbia showed the most resemblance with the profile of pandemic waves in southern Europe, consistent with the southeastern European location of Serbia. The samples were assigned to sixteen SARS-CoV-2 Nextstrain clades: 20A, 20B, 20C, 20D, 20E, 20G, 20I, 21J, 21K, 21L, 22A, 22B, 22C, 22D, 22E, and 22F and six different Omicron recombinants (XZ, XAZ, XAS, XBB, XBF, and XBK). The 10 most common mutations detected in the coding and untranslated regions of the SARS-CoV-2 genomes included four mutations affecting the spike protein (S:D614G, S:T478K, S:P681H, and S:S477N) and one mutation at each of the following positions: 5′-untranslated region (5’UTR:241); N protein (N:RG203KR); NSP3 protein (NSP3:F106F); NSP4 protein (NSP4:T492I); NSP6 protein (NSP6: S106/G107/F108 - triple deletion), and NSP12b protein (NSP12b:P314L). This national-level study is the most comprehensive in terms of sequencing and genomic surveillance of SARS-CoV-2 during the pandemic in Serbia, highlighting the importance of establishing and maintaining good national practice for monitoring SARS-CoV-2 and other viruses circulating worldwide.",
publisher = "Frontiers",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study",
volume = "15",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2327"
}

Novković, M., Banović Đeri, B., RistivojeviĆ, B., Knežević, A., Janković, M., Tanasić, V., Radojičić, V., Keckarević, D., Vidanović, D., Tešović, B., Skakić, A., Tolinački, M., Morić, I.,& Đorđević, V.. (2024). Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study. in Frontiers in Microbiology
Frontiers., 15.
https://hdl.handle.net/21.15107/rcub_imagine_2327

Novković M, Banović Đeri B, RistivojeviĆ B, Knežević A, Janković M, Tanasić V, Radojičić V, Keckarević D, Vidanović D, Tešović B, Skakić A, Tolinački M, Morić I, Đorđević V. Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study. in Frontiers in Microbiology. 2024;15.
https://hdl.handle.net/21.15107/rcub_imagine_2327 .

Novković, Mirjana, Banović Đeri, Bojana, RistivojeviĆ, Bojan, Knežević, Aleksandra, Janković, Marko, Tanasić, Vanja, Radojičić, Verica, Keckarević, Dusan, Vidanović, Dejan, Tešović, Bojana, Skakić, Anita, Tolinački, Maja, Morić, Ivana, Đorđević, Valentina, "Genome sequence diversity of SARS-CoV-2 in Serbia: insights gained from a 3-year pandemic study" in Frontiers in Microbiology, 15 (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2327 .

TY  - JOUR
AU  - Brkušanin, Miloš
AU  - Kosać, Ana
AU  - Branković-Srećković, Vesna
AU  - Jovanović, Kristina
AU  - Perić, Stojan
AU  - Karanović, Jelena
AU  - Matijašević Joković, Suzana
AU  - Garai, Nemanja
AU  - Pešović, Jovan
AU  - Nikolić, Dimitrije
AU  - Stević, Zorica
AU  - Brajušković, Goran
AU  - Milić-Rašić, Vedrana
AU  - Savić-Pavićević, Dušanka
PY  - 2024
UR  - https://www.frontiersin.org/journals/neurology/articles/10.3389/fneur.2024.1394001/full
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2388
AB  - IntroductionBiomarkers capable of reflecting disease onset and short- and long-term therapeutic effects in individuals with spinal muscular atrophy (SMA) are still an unmet need and phosphorylated neurofilament heavy chain (pNF-H) holds significant promise.MethodsWe conducted a longitudinal prospective study to evaluate pNF-H levels in the cerebrospinal fluid (CSF) and plasma of 29 individuals with childhood-onset SMA treated with Nuinersen (SMA type 1: n = 6, 2: n = 17, 3: n = 6). pNF-H levels before and during treatment were compared with the levels of controls (n = 22), patients with Duchenne muscular dystrophy (n = 17), myotonic dystrophy type 1 (n = 11), untreated SMA individuals with chronic type 3 disease (n = 8), and children with presymptomatic SMA (n = 3).ResultsSMA type 1 showed the highest mean CSF pNF-H levels before treatment initiation. All Nusinersen-treated individuals (types 1, 2, and 3) showed significantly elevated mean baseline CSF pNF-H compared to controls, which inversely correlated with age at disease onset, age at first dose, disease duration and the initial CHOP INTEND result (SMA type 1 and 2). During 22 months of treatment, CSF pNF-H levels declined during loading doses, stabilizing at reduced levels from the initial maintenance dose in all individuals. Baseline plasma pNF-H levels in type 1 and 2 SMA were significantly increased compared to other cohorts and decreased notably in type 1 after 2 months of treatment and type 2 after 14 months. Conversely, SMA type 3, characterized by lower baseline pNF-H levels, did not show significant fluctuations in plasma pNF-H levels after 14 months of treatment.ConclusionOur findings suggest that CSF pNF-H levels in untreated SMA individuals are significantly higher than in controls and that monitoring of CSF pNF-H levels may serve as an indicator of rapid short-term treatment response in childhood-onset SMA individuals, irrespective of the subtype of the disease, while also suggesting its potential for assessing long-term suppression of neurodegeneration. Plasma pNF-H may serve as an appropriate outcome measure for disease progression and/or response to treatment in types 1 and 2 but not in type 3. Presymptomatic infants with SMA may show elevated pNF-H levels, confirming early neuronal degeneration.
PB  - Frontiers
T2  - Frontiers in Neurology
T1  - Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia
VL  - 15
DO  - 10.3389/fneur.2024.1394001
ER  - 

@article{
author = "Brkušanin, Miloš and Kosać, Ana and Branković-Srećković, Vesna and Jovanović, Kristina and Perić, Stojan and Karanović, Jelena and Matijašević Joković, Suzana and Garai, Nemanja and Pešović, Jovan and Nikolić, Dimitrije and Stević, Zorica and Brajušković, Goran and Milić-Rašić, Vedrana and Savić-Pavićević, Dušanka",
year = "2024",
abstract = "IntroductionBiomarkers capable of reflecting disease onset and short- and long-term therapeutic effects in individuals with spinal muscular atrophy (SMA) are still an unmet need and phosphorylated neurofilament heavy chain (pNF-H) holds significant promise.MethodsWe conducted a longitudinal prospective study to evaluate pNF-H levels in the cerebrospinal fluid (CSF) and plasma of 29 individuals with childhood-onset SMA treated with Nuinersen (SMA type 1: n = 6, 2: n = 17, 3: n = 6). pNF-H levels before and during treatment were compared with the levels of controls (n = 22), patients with Duchenne muscular dystrophy (n = 17), myotonic dystrophy type 1 (n = 11), untreated SMA individuals with chronic type 3 disease (n = 8), and children with presymptomatic SMA (n = 3).ResultsSMA type 1 showed the highest mean CSF pNF-H levels before treatment initiation. All Nusinersen-treated individuals (types 1, 2, and 3) showed significantly elevated mean baseline CSF pNF-H compared to controls, which inversely correlated with age at disease onset, age at first dose, disease duration and the initial CHOP INTEND result (SMA type 1 and 2). During 22 months of treatment, CSF pNF-H levels declined during loading doses, stabilizing at reduced levels from the initial maintenance dose in all individuals. Baseline plasma pNF-H levels in type 1 and 2 SMA were significantly increased compared to other cohorts and decreased notably in type 1 after 2 months of treatment and type 2 after 14 months. Conversely, SMA type 3, characterized by lower baseline pNF-H levels, did not show significant fluctuations in plasma pNF-H levels after 14 months of treatment.ConclusionOur findings suggest that CSF pNF-H levels in untreated SMA individuals are significantly higher than in controls and that monitoring of CSF pNF-H levels may serve as an indicator of rapid short-term treatment response in childhood-onset SMA individuals, irrespective of the subtype of the disease, while also suggesting its potential for assessing long-term suppression of neurodegeneration. Plasma pNF-H may serve as an appropriate outcome measure for disease progression and/or response to treatment in types 1 and 2 but not in type 3. Presymptomatic infants with SMA may show elevated pNF-H levels, confirming early neuronal degeneration.",
publisher = "Frontiers",
journal = "Frontiers in Neurology",
title = "Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia",
volume = "15",
doi = "10.3389/fneur.2024.1394001"
}

Brkušanin, M., Kosać, A., Branković-Srećković, V., Jovanović, K., Perić, S., Karanović, J., Matijašević Joković, S., Garai, N., Pešović, J., Nikolić, D., Stević, Z., Brajušković, G., Milić-Rašić, V.,& Savić-Pavićević, D.. (2024). Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia. in Frontiers in Neurology
Frontiers., 15.
https://doi.org/10.3389/fneur.2024.1394001

Brkušanin M, Kosać A, Branković-Srećković V, Jovanović K, Perić S, Karanović J, Matijašević Joković S, Garai N, Pešović J, Nikolić D, Stević Z, Brajušković G, Milić-Rašić V, Savić-Pavićević D. Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia. in Frontiers in Neurology. 2024;15.
doi:10.3389/fneur.2024.1394001 .

Brkušanin, Miloš, Kosać, Ana, Branković-Srećković, Vesna, Jovanović, Kristina, Perić, Stojan, Karanović, Jelena, Matijašević Joković, Suzana, Garai, Nemanja, Pešović, Jovan, Nikolić, Dimitrije, Stević, Zorica, Brajušković, Goran, Milić-Rašić, Vedrana, Savić-Pavićević, Dušanka, "Phosphorylated neurofilament heavy chain in cerebrospinal fluid and plasma as a Nusinersen treatment response marker in childhood-onset SMA individuals from Serbia" in Frontiers in Neurology, 15 (2024),
https://doi.org/10.3389/fneur.2024.1394001 . .

TY  - CONF
AU  - Mesaroš, Aleksandra
AU  - Atanasković, Iva
AU  - Jakovljević, Stefan
AU  - Stević, Tatjana
AU  - Dinić, Miroslav
AU  - Lozo, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2152
AB  - Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Determination of hydrogen cyanide producing strains as potential biocontrol agents
EP  - 116
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2152
ER  - 

@conference{
author = "Mesaroš, Aleksandra and Atanasković, Iva and Jakovljević, Stefan and Stević, Tatjana and Dinić, Miroslav and Lozo, Jelena",
year = "2023",
abstract = "Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Determination of hydrogen cyanide producing strains as potential biocontrol agents",
pages = "116-116",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2152"
}

Mesaroš, A., Atanasković, I., Jakovljević, S., Stević, T., Dinić, M.,& Lozo, J.. (2023). Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2152

Mesaroš A, Atanasković I, Jakovljević S, Stević T, Dinić M, Lozo J. Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2152 .

Mesaroš, Aleksandra, Atanasković, Iva, Jakovljević, Stefan, Stević, Tatjana, Dinić, Miroslav, Lozo, Jelena, "Determination of hydrogen cyanide producing strains as potential biocontrol agents" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):116-116,
https://hdl.handle.net/21.15107/rcub_imagine_2152 .

TY  - JOUR
AU  - Novović, Katarina
AU  - Jovčić, Branko
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1786
AB  - : Acinetobacter baumannii is recognized as a clinically significant pathogen causing a wide
spectrum of nosocomial infections. Colistin was considered a last-resort antibiotic for the treatment of
infections caused by multidrug-resistant A. baumannii. Since the reintroduction of colistin, a number
of mechanisms of colistin resistance in A. baumannii have been reported, including complete loss of
LPS by inactivation of the biosynthetic pathway, modifications of target LPS driven by the addition
of phosphoethanolamine (PEtN) moieties to lipid A mediated by the chromosomal pmrCAB operon
and eptA gene-encoded enzymes or plasmid-encoded mcr genes and efflux of colistin from the cell.
In addition to resistance to colistin, widespread heteroresistance is another feature of A. baumannii
that leads to colistin treatment failure. This review aims to present a critical assessment of relevant
published (>50 experimental papers) up-to-date knowledge on the molecular mechanisms of colistin
resistance in A. baumannii with a detailed review of implicated mutations and the global distribution
of colistin-resistant strains.
PB  - MDPI
T2  - Antibiotics
T1  - Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology
IS  - 3
SP  - 516
VL  - 12
DO  - 10.3390/antibiotics12030516
ER  - 

@article{
author = "Novović, Katarina and Jovčić, Branko",
year = "2023",
abstract = ": Acinetobacter baumannii is recognized as a clinically significant pathogen causing a wide
spectrum of nosocomial infections. Colistin was considered a last-resort antibiotic for the treatment of
infections caused by multidrug-resistant A. baumannii. Since the reintroduction of colistin, a number
of mechanisms of colistin resistance in A. baumannii have been reported, including complete loss of
LPS by inactivation of the biosynthetic pathway, modifications of target LPS driven by the addition
of phosphoethanolamine (PEtN) moieties to lipid A mediated by the chromosomal pmrCAB operon
and eptA gene-encoded enzymes or plasmid-encoded mcr genes and efflux of colistin from the cell.
In addition to resistance to colistin, widespread heteroresistance is another feature of A. baumannii
that leads to colistin treatment failure. This review aims to present a critical assessment of relevant
published (>50 experimental papers) up-to-date knowledge on the molecular mechanisms of colistin
resistance in A. baumannii with a detailed review of implicated mutations and the global distribution
of colistin-resistant strains.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology",
number = "3",
pages = "516",
volume = "12",
doi = "10.3390/antibiotics12030516"
}

Novović, K.,& Jovčić, B.. (2023). Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology. in Antibiotics
MDPI., 12(3), 516.
https://doi.org/10.3390/antibiotics12030516

Novović K, Jovčić B. Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology. in Antibiotics. 2023;12(3):516.
doi:10.3390/antibiotics12030516 .

Novović, Katarina, Jovčić, Branko, "Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology" in Antibiotics, 12, no. 3 (2023):516,
https://doi.org/10.3390/antibiotics12030516 . .

TY  - JOUR
AU  - Rajovski, Srećko
AU  - Vučić, Nemanja
AU  - Karanović, Jelena
AU  - Matijašević, Suzana
AU  - Savić-Pavićević, Dušanka
AU  - Dobrijević, Zorana
AU  - Brajušković, Goran
PY  - 2023
UR  - https://doiserbia.nb.rs/Article.aspx?ID=0534-00122301355R
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2212
AB  - PRMT6, PEX10 and SOX5 genetic variants were identified as male infertility-associated loci in a genome-wide association study and further validated in various populations. Still, the results of previous case-control studies varied, which could be due to differences in participants’ ethnic backgrounds. The main purpose of the present study was to evaluate the supposed association of these variants with idiopathic male infertility in North Macedonian population. Furthermore, we aimed to conduct the systematic quantitative data synthesis which includes the results of previous studies on the same issue in other European and non-European populations. A total of 137 men from North Macedonia diagnosed with idiopathic infertility and 130 age-matched fertile controls were included in the present case-control study. PCR-RFLP method was used for genotyping. Meta-analysis was performed by OpenMeta-analyst statistical software. Variants rs10842262 in SOX5, rs2477686 in PEX10 and rs12097821 in PRMT6 showed the lack of statistically significant differences in genotype distributions between men diagnosed with idiopathic infertility and the control group. Still, rs10842262 allele G frequency was significantly increased in men with poor sperm concentration (P= 0.024, OR = 2.10, 95%CI 1.08-4.06). Meta-analysis further showed the association of rs10842262 and rs12097821 with the risk of idiopathic male infertility. Our results obtained in North Macedonian population supported the previous reports on the involvement of rs10842262 in the genetic basis of male infertility. The meta-analysis confirmed the association of rs10842262 and rs12097821 with male infertility occurrence. Still, additional studies are needed to support the present findings.
AB  - Asocijacija varijanti u genima PRMT6, PEX10 i SOX5 sa muškim sterilitetom identifikovana je u studiji genetičke asocijacije na čitavom genomu i kasnije analizirana u studijama slučajeva i kontrola u različitim populacijama. Rezultati prethodnih studija su pokazali značajnu varijabilnost, što može biti posledica razlika u etničkom poreklu studijskih grupa. Osnovni cilj ovog istraživanja je analiza asocijacije navedenih genetičkih varijanti sa rizikom za pojavu idiopatskog muškog steriliteta u populaciji Severne Makedonije. Takođe, naš cilj je bio i sprovođenje sistematske kvantitativne sinteze podataka iz studija sa istom ili sličnom temom istraživanja sprovedenim u drugim evropskim i neevropskim populacijama. Ukupno 137 muškaraca sa idiopatskim sterilitetom iz Severne Makedonije i 130 fertilnih kontrola slične starosti uključeno je u studiju slučajeva i kontrola. Genotipizacaija je vršena PCR-RFLP metodom, dok je za meta-analizu korišćen statistički softver OpenMeta-analyst. Za varijante rs10842262 u SOX5, rs2477686 u PEX10 i rs12097821 u PRMT6 nije utvrđena statistički značajna razlika u distribucijama genotipova između grupe ispitanika sa idiopatskim sterilitetom i kontrolne grupe. Međutim, učestalost alela G varijante rs10842262 bila je značajno povećana kod muškaraca sa niskom koncentracijom spermatozoida (P= 0.024, OR = 2.10, 95%CI 1.08– 4.06). Meta-analizom pokazana je asocijacija rs10842262, ali i rs12097821, sa rizikom za razvoj idiopatskog muškog steriliteta. Naši rezultati ustanovljeni u populaciji Severne Makedonije idu u prilog prethodnim navodima o učešću rs10842262 u genetičkoj osnovi muškog steriliteta. Ipak, dodatne studije su neophodne kako bi potvrdile značaj rezultata ovog istraživanja.
T2  - Genetika
T1  - Association of PRMT6, PEX10 and SOX5 genetic variants with idiopathic male infertility: Evidence from North Macedonian population and an updated meta-analysis
T1  - Asocijacija varijanti u genima PRMT6, PEX10 I SOX5 sa idiopatskim muškim sterilitetom: dokazi iz populacije Severne Makedonije i ažurirana meta-analiza
EP  - 372
IS  - 1
SP  - 355
VL  - 55
DO  - 10.2298/GENSR2301355R
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2212
ER  - 

@article{
author = "Rajovski, Srećko and Vučić, Nemanja and Karanović, Jelena and Matijašević, Suzana and Savić-Pavićević, Dušanka and Dobrijević, Zorana and Brajušković, Goran",
year = "2023",
abstract = "PRMT6, PEX10 and SOX5 genetic variants were identified as male infertility-associated loci in a genome-wide association study and further validated in various populations. Still, the results of previous case-control studies varied, which could be due to differences in participants’ ethnic backgrounds. The main purpose of the present study was to evaluate the supposed association of these variants with idiopathic male infertility in North Macedonian population. Furthermore, we aimed to conduct the systematic quantitative data synthesis which includes the results of previous studies on the same issue in other European and non-European populations. A total of 137 men from North Macedonia diagnosed with idiopathic infertility and 130 age-matched fertile controls were included in the present case-control study. PCR-RFLP method was used for genotyping. Meta-analysis was performed by OpenMeta-analyst statistical software. Variants rs10842262 in SOX5, rs2477686 in PEX10 and rs12097821 in PRMT6 showed the lack of statistically significant differences in genotype distributions between men diagnosed with idiopathic infertility and the control group. Still, rs10842262 allele G frequency was significantly increased in men with poor sperm concentration (P= 0.024, OR = 2.10, 95%CI 1.08-4.06). Meta-analysis further showed the association of rs10842262 and rs12097821 with the risk of idiopathic male infertility. Our results obtained in North Macedonian population supported the previous reports on the involvement of rs10842262 in the genetic basis of male infertility. The meta-analysis confirmed the association of rs10842262 and rs12097821 with male infertility occurrence. Still, additional studies are needed to support the present findings., Asocijacija varijanti u genima PRMT6, PEX10 i SOX5 sa muškim sterilitetom identifikovana je u studiji genetičke asocijacije na čitavom genomu i kasnije analizirana u studijama slučajeva i kontrola u različitim populacijama. Rezultati prethodnih studija su pokazali značajnu varijabilnost, što može biti posledica razlika u etničkom poreklu studijskih grupa. Osnovni cilj ovog istraživanja je analiza asocijacije navedenih genetičkih varijanti sa rizikom za pojavu idiopatskog muškog steriliteta u populaciji Severne Makedonije. Takođe, naš cilj je bio i sprovođenje sistematske kvantitativne sinteze podataka iz studija sa istom ili sličnom temom istraživanja sprovedenim u drugim evropskim i neevropskim populacijama. Ukupno 137 muškaraca sa idiopatskim sterilitetom iz Severne Makedonije i 130 fertilnih kontrola slične starosti uključeno je u studiju slučajeva i kontrola. Genotipizacaija je vršena PCR-RFLP metodom, dok je za meta-analizu korišćen statistički softver OpenMeta-analyst. Za varijante rs10842262 u SOX5, rs2477686 u PEX10 i rs12097821 u PRMT6 nije utvrđena statistički značajna razlika u distribucijama genotipova između grupe ispitanika sa idiopatskim sterilitetom i kontrolne grupe. Međutim, učestalost alela G varijante rs10842262 bila je značajno povećana kod muškaraca sa niskom koncentracijom spermatozoida (P= 0.024, OR = 2.10, 95%CI 1.08– 4.06). Meta-analizom pokazana je asocijacija rs10842262, ali i rs12097821, sa rizikom za razvoj idiopatskog muškog steriliteta. Naši rezultati ustanovljeni u populaciji Severne Makedonije idu u prilog prethodnim navodima o učešću rs10842262 u genetičkoj osnovi muškog steriliteta. Ipak, dodatne studije su neophodne kako bi potvrdile značaj rezultata ovog istraživanja.",
journal = "Genetika",
title = "Association of PRMT6, PEX10 and SOX5 genetic variants with idiopathic male infertility: Evidence from North Macedonian population and an updated meta-analysis, Asocijacija varijanti u genima PRMT6, PEX10 I SOX5 sa idiopatskim muškim sterilitetom: dokazi iz populacije Severne Makedonije i ažurirana meta-analiza",
pages = "372-355",
number = "1",
volume = "55",
doi = "10.2298/GENSR2301355R",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2212"
}

Rajovski, S., Vučić, N., Karanović, J., Matijašević, S., Savić-Pavićević, D., Dobrijević, Z.,& Brajušković, G.. (2023). Association of PRMT6, PEX10 and SOX5 genetic variants with idiopathic male infertility: Evidence from North Macedonian population and an updated meta-analysis. in Genetika, 55(1), 355-372.
https://doi.org/10.2298/GENSR2301355R
https://hdl.handle.net/21.15107/rcub_imagine_2212

Rajovski S, Vučić N, Karanović J, Matijašević S, Savić-Pavićević D, Dobrijević Z, Brajušković G. Association of PRMT6, PEX10 and SOX5 genetic variants with idiopathic male infertility: Evidence from North Macedonian population and an updated meta-analysis. in Genetika. 2023;55(1):355-372.
doi:10.2298/GENSR2301355R
https://hdl.handle.net/21.15107/rcub_imagine_2212 .

Rajovski, Srećko, Vučić, Nemanja, Karanović, Jelena, Matijašević, Suzana, Savić-Pavićević, Dušanka, Dobrijević, Zorana, Brajušković, Goran, "Association of PRMT6, PEX10 and SOX5 genetic variants with idiopathic male infertility: Evidence from North Macedonian population and an updated meta-analysis" in Genetika, 55, no. 1 (2023):355-372,
https://doi.org/10.2298/GENSR2301355R .,
https://hdl.handle.net/21.15107/rcub_imagine_2212 .

TY  - CONF
AU  - Karanović, Jelena
AU  - Beraković, Doroteja
AU  - Katrašnik, Mojca
AU  - Pantović-Stefanović, Maja
AU  - Radenković, Lana
AU  - Garai, Nemanja
AU  - Ivković, Maja
AU  - Savić-Pavićević, Dušanka
AU  - Zupanc, Tomaž
AU  - Videtič Paska, Alja
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2197
AB  - Introduction: Suicidal behavior ranges between suicidal ideation and completed suicide. Completed
suicide accounts for over 700,000 deaths worldwide, while attempted suicide is 20 times more frequent.
Genetic background is an important factor contributing to suicidal behavior, and candidate genes linked
to several neurotransmitter systems have been investigated. Alternations in glutamate, γ-aminobutyric
acid (GABA) and polyamine systems have been detected in suicidal behavior. Our aim was to differentiate
genetic predispositions underlying two different types of suicidal behavior, attempted and completed
suicide, in two Balkan populations.
Methods: The study sample included 173 suicide attempters with comorbid psychiatric disorders (major
depressive disorder, bipolar affective disorder, or schizophrenia), 216 non-suicidal psychiatric patients and
172 healthy controls from Serbia, and 333 suicide completers and 356 non-suicidal autopsy controls
from Slovenia. Variants in the genes GRIN2B (rs2268115 and rs220557), GABRG2 (rs424740), and ODC1
(rs1049500 and rs2302614) were genotyped by TaqMan assays and analyzed using PLINK.
Results: The CA genotype of rs220557 in the GRIN2B gene increases the risk for completed suicide
(OR=1.51, p=0.021), and particularly violent suicide (OR=1.49, p=0.037), compared to controls. In the
ODC1 gene, the CA genotype of rs2302614 decreases the risk for completed suicide compared to suicide
attempt (OR=0.32, p=0.012). Marginally, the AC haplotype for variants rs1049500-rs2302614 in the ODC1
gene decreases the risk for completed suicide compared to suicide attempt (OR=0.50, p=0.052).
Conclusion: Specific genetic variants of the glutamate and the polyamine systems are differently distributed
among diverse suicidal phenotypes, thus providing further information on the implication of
these systems in suicidality.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Genetic predisposition of suicidal behavior: variants in GRIN2B, GABRG2, and ODC1 genes in suicide attempt and completed suicide In two Balkan populations
EP  - 50
SP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2197
ER  - 

@conference{
author = "Karanović, Jelena and Beraković, Doroteja and Katrašnik, Mojca and Pantović-Stefanović, Maja and Radenković, Lana and Garai, Nemanja and Ivković, Maja and Savić-Pavićević, Dušanka and Zupanc, Tomaž and Videtič Paska, Alja",
year = "2023",
abstract = "Introduction: Suicidal behavior ranges between suicidal ideation and completed suicide. Completed
suicide accounts for over 700,000 deaths worldwide, while attempted suicide is 20 times more frequent.
Genetic background is an important factor contributing to suicidal behavior, and candidate genes linked
to several neurotransmitter systems have been investigated. Alternations in glutamate, γ-aminobutyric
acid (GABA) and polyamine systems have been detected in suicidal behavior. Our aim was to differentiate
genetic predispositions underlying two different types of suicidal behavior, attempted and completed
suicide, in two Balkan populations.
Methods: The study sample included 173 suicide attempters with comorbid psychiatric disorders (major
depressive disorder, bipolar affective disorder, or schizophrenia), 216 non-suicidal psychiatric patients and
172 healthy controls from Serbia, and 333 suicide completers and 356 non-suicidal autopsy controls
from Slovenia. Variants in the genes GRIN2B (rs2268115 and rs220557), GABRG2 (rs424740), and ODC1
(rs1049500 and rs2302614) were genotyped by TaqMan assays and analyzed using PLINK.
Results: The CA genotype of rs220557 in the GRIN2B gene increases the risk for completed suicide
(OR=1.51, p=0.021), and particularly violent suicide (OR=1.49, p=0.037), compared to controls. In the
ODC1 gene, the CA genotype of rs2302614 decreases the risk for completed suicide compared to suicide
attempt (OR=0.32, p=0.012). Marginally, the AC haplotype for variants rs1049500-rs2302614 in the ODC1
gene decreases the risk for completed suicide compared to suicide attempt (OR=0.50, p=0.052).
Conclusion: Specific genetic variants of the glutamate and the polyamine systems are differently distributed
among diverse suicidal phenotypes, thus providing further information on the implication of
these systems in suicidality.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Genetic predisposition of suicidal behavior: variants in GRIN2B, GABRG2, and ODC1 genes in suicide attempt and completed suicide In two Balkan populations",
pages = "50-50",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2197"
}

Karanović, J., Beraković, D., Katrašnik, M., Pantović-Stefanović, M., Radenković, L., Garai, N., Ivković, M., Savić-Pavićević, D., Zupanc, T.,& Videtič Paska, A.. (2023). Genetic predisposition of suicidal behavior: variants in GRIN2B, GABRG2, and ODC1 genes in suicide attempt and completed suicide In two Balkan populations. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 50-50.
https://hdl.handle.net/21.15107/rcub_imagine_2197

Karanović J, Beraković D, Katrašnik M, Pantović-Stefanović M, Radenković L, Garai N, Ivković M, Savić-Pavićević D, Zupanc T, Videtič Paska A. Genetic predisposition of suicidal behavior: variants in GRIN2B, GABRG2, and ODC1 genes in suicide attempt and completed suicide In two Balkan populations. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:50-50.
https://hdl.handle.net/21.15107/rcub_imagine_2197 .

Karanović, Jelena, Beraković, Doroteja, Katrašnik, Mojca, Pantović-Stefanović, Maja, Radenković, Lana, Garai, Nemanja, Ivković, Maja, Savić-Pavićević, Dušanka, Zupanc, Tomaž, Videtič Paska, Alja, "Genetic predisposition of suicidal behavior: variants in GRIN2B, GABRG2, and ODC1 genes in suicide attempt and completed suicide In two Balkan populations" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):50-50,
https://hdl.handle.net/21.15107/rcub_imagine_2197 .

TY  - CONF
AU  - Garai, Nemanja
AU  - Petrović, Kristina
AU  - Karanović, Jelena
AU  - Dejanović, Ivana
AU  - Perić, Stojan
AU  - Basta, Ivana
AU  - Jovanović, Vladimir
AU  - Savić-Pavićević, Dušanka
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2196
AB  - Introduction: Genome-wide association studies (GWAS) identify genomic loci that contain genetic determinants
of complex diseases. Subsequent functional genomic approaches, such as bioinformatic finemapping
and transcriptome-wide association studies (TWAS), can reveal potentially causal single
nucleotide variants (SNVs) that can be tested on patient samples. We applied this approach to study
causal SNVs for acetylcholine receptor (AChR) seropositive myasthenia gravis (MG). We focused on
CHRNA1 and CHRNB1 loci, coding AChR subunits, and CTLA-4 locus, coding protein transmitting an inhibitory
signal to T cells.
Methods: CHRNA1 was fine-mapped by PAINTOR using data from GWAS summary statistics, 1000
genome and RegulomeDB. Alongside, rs4151121 identified by TWAS in CHRNB1, and rs231735 and
rs231770 identified by fine-mapping in CTLA-4 were studied. SNVs were genotyped using allele discrimination
assays in 447 Serbian AChR-MG patients (183 early-onset and 264 late-onset) and 447 sex- and
age-matched controls.
Results: CHRNA1 rs35274388 was fine-mapped as a potentially causal variant (PIP2=92%) exhibiting
transcription factor binding and chromatin accessibility peaks. CHRNA1 rs35274388 minor allele A and
CHRNAB1 rs4151121 minor allele G increased the risk for late-onset MG (OR=1.669, 95% CI=1.05-2.638,
p=0.027, p10e6 permutation=0.031 and OR=1.322, 95% CI=1.063-1.644, p10e6 permutation=0.014, respectively).
On the other hand, CTLA-4 rs231735 recessive genotype TT decreased, while rs231735-
rs231770 haplotype GC increased the susceptibility to early-onset MG (OR=0.548, 95% CI=0.339-0.888,
p=0.014, p10e6 permutation=0.014 and OR=1.360, p=0.027, p10e6 permutation=0.027, respectively).
Conclusion: CHRNA1 rs35274388 and CHRNAB1 rs4151121 loci could be causal genetic factors for lateonset
MG while CTLA-4 rs231735 and rs231770 could be causal genetic factors for early-onset MG in Serbian
population.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Identification of potentally causal variants for myasthenia gravis: a bioinformatics-driven fine-mapping approach combined with genetic association study
EP  - 39
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2196
ER  - 

@conference{
author = "Garai, Nemanja and Petrović, Kristina and Karanović, Jelena and Dejanović, Ivana and Perić, Stojan and Basta, Ivana and Jovanović, Vladimir and Savić-Pavićević, Dušanka",
year = "2023",
abstract = "Introduction: Genome-wide association studies (GWAS) identify genomic loci that contain genetic determinants
of complex diseases. Subsequent functional genomic approaches, such as bioinformatic finemapping
and transcriptome-wide association studies (TWAS), can reveal potentially causal single
nucleotide variants (SNVs) that can be tested on patient samples. We applied this approach to study
causal SNVs for acetylcholine receptor (AChR) seropositive myasthenia gravis (MG). We focused on
CHRNA1 and CHRNB1 loci, coding AChR subunits, and CTLA-4 locus, coding protein transmitting an inhibitory
signal to T cells.
Methods: CHRNA1 was fine-mapped by PAINTOR using data from GWAS summary statistics, 1000
genome and RegulomeDB. Alongside, rs4151121 identified by TWAS in CHRNB1, and rs231735 and
rs231770 identified by fine-mapping in CTLA-4 were studied. SNVs were genotyped using allele discrimination
assays in 447 Serbian AChR-MG patients (183 early-onset and 264 late-onset) and 447 sex- and
age-matched controls.
Results: CHRNA1 rs35274388 was fine-mapped as a potentially causal variant (PIP2=92%) exhibiting
transcription factor binding and chromatin accessibility peaks. CHRNA1 rs35274388 minor allele A and
CHRNAB1 rs4151121 minor allele G increased the risk for late-onset MG (OR=1.669, 95% CI=1.05-2.638,
p=0.027, p10e6 permutation=0.031 and OR=1.322, 95% CI=1.063-1.644, p10e6 permutation=0.014, respectively).
On the other hand, CTLA-4 rs231735 recessive genotype TT decreased, while rs231735-
rs231770 haplotype GC increased the susceptibility to early-onset MG (OR=0.548, 95% CI=0.339-0.888,
p=0.014, p10e6 permutation=0.014 and OR=1.360, p=0.027, p10e6 permutation=0.027, respectively).
Conclusion: CHRNA1 rs35274388 and CHRNAB1 rs4151121 loci could be causal genetic factors for lateonset
MG while CTLA-4 rs231735 and rs231770 could be causal genetic factors for early-onset MG in Serbian
population.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Identification of potentally causal variants for myasthenia gravis: a bioinformatics-driven fine-mapping approach combined with genetic association study",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2196"
}

Garai, N., Petrović, K., Karanović, J., Dejanović, I., Perić, S., Basta, I., Jovanović, V.,& Savić-Pavićević, D.. (2023). Identification of potentally causal variants for myasthenia gravis: a bioinformatics-driven fine-mapping approach combined with genetic association study. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 39-39.
https://hdl.handle.net/21.15107/rcub_imagine_2196

Garai N, Petrović K, Karanović J, Dejanović I, Perić S, Basta I, Jovanović V, Savić-Pavićević D. Identification of potentally causal variants for myasthenia gravis: a bioinformatics-driven fine-mapping approach combined with genetic association study. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:39-39.
https://hdl.handle.net/21.15107/rcub_imagine_2196 .

Garai, Nemanja, Petrović, Kristina, Karanović, Jelena, Dejanović, Ivana, Perić, Stojan, Basta, Ivana, Jovanović, Vladimir, Savić-Pavićević, Dušanka, "Identification of potentally causal variants for myasthenia gravis: a bioinformatics-driven fine-mapping approach combined with genetic association study" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):39-39,
https://hdl.handle.net/21.15107/rcub_imagine_2196 .

TY  - DATA
AU  - Kabić, Jovana
AU  - Novović, Katarina
AU  - Kekić, Dušan
AU  - Trudić, Anika
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Jovčić, Branko
AU  - Gajić, Ina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1785
T2  - Computational and Structural Biotechnology Journal
T2  - Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal
T1  - Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1785
ER  - 

@misc{
author = "Kabić, Jovana and Novović, Katarina and Kekić, Dušan and Trudić, Anika and Opavski, Nataša and Dimkić, Ivica and Jovčić, Branko and Gajić, Ina",
year = "2023",
journal = "Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal",
title = "Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1785"
}

Kabić, J., Novović, K., Kekić, D., Trudić, A., Opavski, N., Dimkić, I., Jovčić, B.,& Gajić, I.. (2023). Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045. in Computational and Structural Biotechnology Journal.
https://hdl.handle.net/21.15107/rcub_imagine_1785

Kabić J, Novović K, Kekić D, Trudić A, Opavski N, Dimkić I, Jovčić B, Gajić I. Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045. in Computational and Structural Biotechnology Journal. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1785 .

Kabić, Jovana, Novović, Katarina, Kekić, Dušan, Trudić, Anika, Opavski, Nataša, Dimkić, Ivica, Jovčić, Branko, Gajić, Ina, "Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045" in Computational and Structural Biotechnology Journal (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1785 .

TY  - JOUR
AU  - Kabić, Jovana
AU  - Novović, Katarina
AU  - Kekić, Dušan
AU  - Trudić, Anika
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Jovčić, Branko
AU  - Gajić, Ina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S200103702200602X
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1784
AB  - This study aimed to investigate the prevalence and resistance mechanisms of colistin-resistant Acinetobacter baumannii (ColRAB) isolates in Serbia, assess their genetic relatedness to other circulating A. baumannii isolates in the neighbouring European countries, and analyse the global genomic epidemiology of ColRAB isolates. A total of 784 isolates of A. baumannii were recovered from hospitalised patients in Serbia between 2018 and 2021. The antimicrobial susceptibility testing was performed using disk diffusion and broth microdilution. All ColRAB isolates were subjected to DNA isolation and whole-genome sequencing (WGS). Overall, 3.94 % (n = 30) isolates were confirmed as ColRAB. Results of mutational and transcriptional analysis of genes associated with colistin resistance indicate the central role of the two-component regulating system, PmrAB, and increased expression of the pmrC gene in ColRAB. Most of the isolates (n = 29, 96.6 %) belonged to international clone II, with the most common sequence type being STPas2 (n = 23, 76.6 %). Based on the WGS analysis, ColRAB isolates belonging to the same ST isolated in various countries were grouped into the same clusters, indicating the global dissemination of several high-risk clonal lineages. Phylogenomic analysis of ColRAB isolates, together with all previously published A. baumannii genomes from South-Eastern European countries, showed that colistin resistance arose independently in several clonal lineages. Comparative genomic analysis revealed multiple genes with various roles (transcriptional regulation, transmembrane transport, outer membrane assembly, etc.), which might be associated with colistin resistance in A. baumannii. The obtained findings serve as the basis for further studies, contributing to a better understanding of colistin resistance mechanisms in A. baumannii.
T2  - Computational and Structural Biotechnology Journal
T2  - Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal
T1  - Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii
EP  - 585
SP  - 574
VL  - 21
DO  - 10.1016/j.csbj.2022.12.045
DO  - 10.1016/j.csbj.2022.12.045
ER  - 

@article{
author = "Kabić, Jovana and Novović, Katarina and Kekić, Dušan and Trudić, Anika and Opavski, Nataša and Dimkić, Ivica and Jovčić, Branko and Gajić, Ina",
year = "2023",
abstract = "This study aimed to investigate the prevalence and resistance mechanisms of colistin-resistant Acinetobacter baumannii (ColRAB) isolates in Serbia, assess their genetic relatedness to other circulating A. baumannii isolates in the neighbouring European countries, and analyse the global genomic epidemiology of ColRAB isolates. A total of 784 isolates of A. baumannii were recovered from hospitalised patients in Serbia between 2018 and 2021. The antimicrobial susceptibility testing was performed using disk diffusion and broth microdilution. All ColRAB isolates were subjected to DNA isolation and whole-genome sequencing (WGS). Overall, 3.94 % (n = 30) isolates were confirmed as ColRAB. Results of mutational and transcriptional analysis of genes associated with colistin resistance indicate the central role of the two-component regulating system, PmrAB, and increased expression of the pmrC gene in ColRAB. Most of the isolates (n = 29, 96.6 %) belonged to international clone II, with the most common sequence type being STPas2 (n = 23, 76.6 %). Based on the WGS analysis, ColRAB isolates belonging to the same ST isolated in various countries were grouped into the same clusters, indicating the global dissemination of several high-risk clonal lineages. Phylogenomic analysis of ColRAB isolates, together with all previously published A. baumannii genomes from South-Eastern European countries, showed that colistin resistance arose independently in several clonal lineages. Comparative genomic analysis revealed multiple genes with various roles (transcriptional regulation, transmembrane transport, outer membrane assembly, etc.), which might be associated with colistin resistance in A. baumannii. The obtained findings serve as the basis for further studies, contributing to a better understanding of colistin resistance mechanisms in A. baumannii.",
journal = "Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal",
title = "Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii",
pages = "585-574",
volume = "21",
doi = "10.1016/j.csbj.2022.12.045, 10.1016/j.csbj.2022.12.045"
}

Kabić, J., Novović, K., Kekić, D., Trudić, A., Opavski, N., Dimkić, I., Jovčić, B.,& Gajić, I.. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. in Computational and Structural Biotechnology Journal, 21, 574-585.
https://doi.org/10.1016/j.csbj.2022.12.045

Kabić J, Novović K, Kekić D, Trudić A, Opavski N, Dimkić I, Jovčić B, Gajić I. Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. in Computational and Structural Biotechnology Journal. 2023;21:574-585.
doi:10.1016/j.csbj.2022.12.045 .

Kabić, Jovana, Novović, Katarina, Kekić, Dušan, Trudić, Anika, Opavski, Nataša, Dimkić, Ivica, Jovčić, Branko, Gajić, Ina, "Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii" in Computational and Structural Biotechnology Journal, 21 (2023):574-585,
https://doi.org/10.1016/j.csbj.2022.12.045 . .

TY  - DATA
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Poledica, Mirjana
AU  - Nikolić, Gordana
AU  - Grujić, Bojana
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Filipić, Brankica
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1783
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1783
ER  - 

@misc{
author = "Novović, Katarina and Kuzmanović Nedeljković, Snežana and Poledica, Mirjana and Nikolić, Gordana and Grujić, Bojana and Jovčić, Branko and Kojić, Milan and Filipić, Brankica",
year = "2023",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1783"
}

Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M.,& Filipić, B.. (2023). Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184. in Frontiers in Microbiology.
https://hdl.handle.net/21.15107/rcub_imagine_1783

Novović K, Kuzmanović Nedeljković S, Poledica M, Nikolić G, Grujić B, Jovčić B, Kojić M, Filipić B. Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184. in Frontiers in Microbiology. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1783 .

Novović, Katarina, Kuzmanović Nedeljković, Snežana, Poledica, Mirjana, Nikolić, Gordana, Grujić, Bojana, Jovčić, Branko, Kojić, Milan, Filipić, Brankica, "Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184" in Frontiers in Microbiology (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1783 .

TY  - CONF
AU  - Ćurčić, Jovana
AU  - Jakovljević, Stefan
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Malešević, Milka
PY  - 2023
UR  - http://intor.torlakinstitut.com/handle/123456789/803
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2124
AB  - Introduction: Quorum quenching (QQ) isthe enzymatic degradation of cell-to-cellsignaling molecules.In this study, the potential of the novel YtnP lactonase, the quorum quenching enzyme derived from S.maltophilia, to reduce P. aeruginosa quorum sensing and virulence factor gene expression was investigated.Methods: MMA83 culture (adjusted to 1.5x105 CFU/ml) was treated with recombinant YtnP lactonase(final concentration 50 μg/ml) at 37°C for 12 hours under aeration. RNA isolation of the treated and untreated MMA83 culture was performed using the RNeasy Mini Kit (Qiagen, Germany) according to theprotocol. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR), was used to analyzethe effect ofYtnP lactonase on the relative mRNA levels of the LasI/LasR, RhiI/RhiR, and PQS signaling network genes of P. aeruginosa MMA83 and virulence factor genes. The rpsL was used as an endogenouscontrol to normalize obtained data following the 2-ΔΔCt method.Results: The QS genes belonging to three QS networks – LasI/LasR, RhiI/RhiR, and PQS of P. aeruginosaMMA83 treated with YtnP lactonase were significantly downregulated. The RT -qPCR results show thattreatment with YtnP-lactonase decreased the relative mRNA levels of genes involved in the productionof elastase (lasB approximately 2-fold), alginate (algK approximately 2.2-fold), pyocyanin (phzM approximately 3.5-fold), pyoverdin (pvdS approximately 2-fold), and rhamnolipid (rhlC approximately 4-fold).These results suggest that YtnP lactonase exerts an antivirulence effect at the transcription level.Conclusion: YtnP lactonase, a quorum quenching (QQ) enzyme, has the potential to be used as an innovative enzyme-based antivirulence therapeutic to combat infections caused by P. aeruginosa.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression
EP  - 121
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2124
ER  - 

@conference{
author = "Ćurčić, Jovana and Jakovljević, Stefan and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Malešević, Milka",
year = "2023",
abstract = "Introduction: Quorum quenching (QQ) isthe enzymatic degradation of cell-to-cellsignaling molecules.In this study, the potential of the novel YtnP lactonase, the quorum quenching enzyme derived from S.maltophilia, to reduce P. aeruginosa quorum sensing and virulence factor gene expression was investigated.Methods: MMA83 culture (adjusted to 1.5x105 CFU/ml) was treated with recombinant YtnP lactonase(final concentration 50 μg/ml) at 37°C for 12 hours under aeration. RNA isolation of the treated and untreated MMA83 culture was performed using the RNeasy Mini Kit (Qiagen, Germany) according to theprotocol. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR), was used to analyzethe effect ofYtnP lactonase on the relative mRNA levels of the LasI/LasR, RhiI/RhiR, and PQS signaling network genes of P. aeruginosa MMA83 and virulence factor genes. The rpsL was used as an endogenouscontrol to normalize obtained data following the 2-ΔΔCt method.Results: The QS genes belonging to three QS networks – LasI/LasR, RhiI/RhiR, and PQS of P. aeruginosaMMA83 treated with YtnP lactonase were significantly downregulated. The RT -qPCR results show thattreatment with YtnP-lactonase decreased the relative mRNA levels of genes involved in the productionof elastase (lasB approximately 2-fold), alginate (algK approximately 2.2-fold), pyocyanin (phzM approximately 3.5-fold), pyoverdin (pvdS approximately 2-fold), and rhamnolipid (rhlC approximately 4-fold).These results suggest that YtnP lactonase exerts an antivirulence effect at the transcription level.Conclusion: YtnP lactonase, a quorum quenching (QQ) enzyme, has the potential to be used as an innovative enzyme-based antivirulence therapeutic to combat infections caused by P. aeruginosa.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2124"
}

Ćurčić, J., Jakovljević, S., Novović, K., Vasiljević, Z., Kojić, M., Jovčić, B.,& Malešević, M.. (2023). A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2124

Ćurčić J, Jakovljević S, Novović K, Vasiljević Z, Kojić M, Jovčić B, Malešević M. A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2124 .

Ćurčić, Jovana, Jakovljević, Stefan, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Malešević, Milka, "A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):121-121,
https://hdl.handle.net/21.15107/rcub_imagine_2124 .

TY  - CONF
AU  - Šaraba, Vladimir
AU  - Nikodinović-Runić, Jasmina
AU  - Obradović, Vesna
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Ćirić, Milica
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2104
AB  - Targeted physiological groups of bacteria were cultivated and identified in the brackish
groundwaters of Obrenovačka Banja (OB), Lomnički Kiseljak (LK) and Velika Vrbnica (VV) using biological
activity reaction tests (BARTs) to assess the biocorrosion, biofouling and health risks. The highest density of
iron-related, sulfate-reducing, slime-forming, facultatively anaerobic heterotrophic, denitrifying bacteria and
representatives of Pseudomonas spp. was recorded in the OB sample, while the lowest density of the same
physiological groups of bacteria was recorded in the LK sample. Facultatively anaerobic heterotrophic
bacteria were the most abundant in the OB and LK samples, while, in contrast, heterotrophic aerobic bacteria
were the most abundant in the VV sample. All tested samples were characterized by a high degree of
biochemical activity associated with iron-related, sulfate-reducing, slime-forming, heterotrophic aerobic and
facultatively anaerobic bacteria. Also, high biochemical activity of denitrifying bacteria was recorded in the
OB sample, and the same activity of Pseudomonas species was recorded in the OB and VV samples. For OB
and LK groundwaters, the highest degree of risk was estimated for biocorrosion process, while for the OB
and VV occurrences, the highest degree of risk was estimated for biofouling process. The health risk was
present for all examined groundwaters. Caution is warranted in further use of all investigated occurrences
due to the established public health risk and an immediate revitalization of the OB, LK and VV wells is
necessary.
PB  - Kragujevac : Institute for Information Technologies
C3  - 2nd International Conference on Chemo and Bioinformatics
T1  - Biocorrosion, biofouling and health risk: biological activity reaction tests of selected brackish groundwater occurrences in Serbia
EP  - 190
SP  - 186
DO  - 10.46793/ICCBI23.086S
ER  - 

@conference{
author = "Šaraba, Vladimir and Nikodinović-Runić, Jasmina and Obradović, Vesna and Dimkić, Ivica and Janakiev, Tamara and Ćirić, Milica",
year = "2023",
abstract = "Targeted physiological groups of bacteria were cultivated and identified in the brackish
groundwaters of Obrenovačka Banja (OB), Lomnički Kiseljak (LK) and Velika Vrbnica (VV) using biological
activity reaction tests (BARTs) to assess the biocorrosion, biofouling and health risks. The highest density of
iron-related, sulfate-reducing, slime-forming, facultatively anaerobic heterotrophic, denitrifying bacteria and
representatives of Pseudomonas spp. was recorded in the OB sample, while the lowest density of the same
physiological groups of bacteria was recorded in the LK sample. Facultatively anaerobic heterotrophic
bacteria were the most abundant in the OB and LK samples, while, in contrast, heterotrophic aerobic bacteria
were the most abundant in the VV sample. All tested samples were characterized by a high degree of
biochemical activity associated with iron-related, sulfate-reducing, slime-forming, heterotrophic aerobic and
facultatively anaerobic bacteria. Also, high biochemical activity of denitrifying bacteria was recorded in the
OB sample, and the same activity of Pseudomonas species was recorded in the OB and VV samples. For OB
and LK groundwaters, the highest degree of risk was estimated for biocorrosion process, while for the OB
and VV occurrences, the highest degree of risk was estimated for biofouling process. The health risk was
present for all examined groundwaters. Caution is warranted in further use of all investigated occurrences
due to the established public health risk and an immediate revitalization of the OB, LK and VV wells is
necessary.",
publisher = "Kragujevac : Institute for Information Technologies",
journal = "2nd International Conference on Chemo and Bioinformatics",
title = "Biocorrosion, biofouling and health risk: biological activity reaction tests of selected brackish groundwater occurrences in Serbia",
pages = "190-186",
doi = "10.46793/ICCBI23.086S"
}

Šaraba, V., Nikodinović-Runić, J., Obradović, V., Dimkić, I., Janakiev, T.,& Ćirić, M.. (2023). Biocorrosion, biofouling and health risk: biological activity reaction tests of selected brackish groundwater occurrences in Serbia. in 2nd International Conference on Chemo and Bioinformatics
Kragujevac : Institute for Information Technologies., 186-190.
https://doi.org/10.46793/ICCBI23.086S

Šaraba V, Nikodinović-Runić J, Obradović V, Dimkić I, Janakiev T, Ćirić M. Biocorrosion, biofouling and health risk: biological activity reaction tests of selected brackish groundwater occurrences in Serbia. in 2nd International Conference on Chemo and Bioinformatics. 2023;:186-190.
doi:10.46793/ICCBI23.086S .

Šaraba, Vladimir, Nikodinović-Runić, Jasmina, Obradović, Vesna, Dimkić, Ivica, Janakiev, Tamara, Ćirić, Milica, "Biocorrosion, biofouling and health risk: biological activity reaction tests of selected brackish groundwater occurrences in Serbia" in 2nd International Conference on Chemo and Bioinformatics (2023):186-190,
https://doi.org/10.46793/ICCBI23.086S . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Poledica, Mirjana
AU  - Nikolić, Gordana
AU  - Grujić, Bojana
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Filipić, Brankica
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1782
AB  - Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia
VL  - 14
DO  - 10.3389/fmicb.2023.1094184
ER  - 

@article{
author = "Novović, Katarina and Kuzmanović Nedeljković, Snežana and Poledica, Mirjana and Nikolić, Gordana and Grujić, Bojana and Jovčić, Branko and Kojić, Milan and Filipić, Brankica",
year = "2023",
abstract = "Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia",
volume = "14",
doi = "10.3389/fmicb.2023.1094184"
}

Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M.,& Filipić, B.. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology, 14.
https://doi.org/10.3389/fmicb.2023.1094184

Novović K, Kuzmanović Nedeljković S, Poledica M, Nikolić G, Grujić B, Jovčić B, Kojić M, Filipić B. Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology. 2023;14.
doi:10.3389/fmicb.2023.1094184 .

Novović, Katarina, Kuzmanović Nedeljković, Snežana, Poledica, Mirjana, Nikolić, Gordana, Grujić, Bojana, Jovčić, Branko, Kojić, Milan, Filipić, Brankica, "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia" in Frontiers in Microbiology, 14 (2023),
https://doi.org/10.3389/fmicb.2023.1094184 . .

TY  - JOUR
AU  - Obradović, Mina
AU  - Malešević, Milka
AU  - Di Luca, Mariagrazia
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - McAuliffe, Olivia
AU  - Neve, Horst
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2023
UR  - https://www.mdpi.com/1999-4915/15/3/628
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2065
AB  - Klebsiella pneumoniae is a global health threat and bacteriophages are a potential solution in combating pandrug-resistant K. pneumoniae infections. Two lytic phages, LASTA and SJM3, active against several pandrug-resistant, nosocomial strains of K. pneumoniae were isolated and characterized. Their host range is narrow and latent period is particularly long; however, their lysogenic nature was refuted using both bioinformatic and experimental approaches. Genome sequence analysis clustered them with only two other phages into the new genus Lastavirus. Genomes of LASTA and SJM3 differ in only 13 base pairs, mainly located in tail fiber genes. Individual phages, as well as their cocktail, demonstrated significant bacterial reduction capacity in a time-dependent manner, yielding up to 4 log reduction against planktonic, and up to 2.59 log on biofilm-embedded, cells. Bacteria emerging from the contact with the phages developed resistance and achieved numbers comparable to the growth control after 24 h. The resistance to the phage seems to be of a transient nature and varies significantly between the two phages, as resistance to LASTA remained constant while resensitization to SJM3 was more prominent. Albeit with very few differences, SJM3 performed better than LASTA overall; however, more investigation is needed in order to consider them for therapeutic application.
T2  - Viruses
T1  - Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae
IS  - 3
SP  - 628
VL  - 15
DO  - 10.3390/v15030628
ER  - 

@article{
author = "Obradović, Mina and Malešević, Milka and Di Luca, Mariagrazia and Kekić, Dušan and Gajić, Ina and McAuliffe, Olivia and Neve, Horst and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan",
year = "2023",
abstract = "Klebsiella pneumoniae is a global health threat and bacteriophages are a potential solution in combating pandrug-resistant K. pneumoniae infections. Two lytic phages, LASTA and SJM3, active against several pandrug-resistant, nosocomial strains of K. pneumoniae were isolated and characterized. Their host range is narrow and latent period is particularly long; however, their lysogenic nature was refuted using both bioinformatic and experimental approaches. Genome sequence analysis clustered them with only two other phages into the new genus Lastavirus. Genomes of LASTA and SJM3 differ in only 13 base pairs, mainly located in tail fiber genes. Individual phages, as well as their cocktail, demonstrated significant bacterial reduction capacity in a time-dependent manner, yielding up to 4 log reduction against planktonic, and up to 2.59 log on biofilm-embedded, cells. Bacteria emerging from the contact with the phages developed resistance and achieved numbers comparable to the growth control after 24 h. The resistance to the phage seems to be of a transient nature and varies significantly between the two phages, as resistance to LASTA remained constant while resensitization to SJM3 was more prominent. Albeit with very few differences, SJM3 performed better than LASTA overall; however, more investigation is needed in order to consider them for therapeutic application.",
journal = "Viruses",
title = "Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae",
number = "3",
pages = "628",
volume = "15",
doi = "10.3390/v15030628"
}

Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628.
https://doi.org/10.3390/v15030628

Obradović M, Malešević M, Di Luca M, Kekić D, Gajić I, McAuliffe O, Neve H, Stanisavljević N, Vukotić G, Kojić M. Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses. 2023;15(3):628.
doi:10.3390/v15030628 .

Obradović, Mina, Malešević, Milka, Di Luca, Mariagrazia, Kekić, Dušan, Gajić, Ina, McAuliffe, Olivia, Neve, Horst, Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, "Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae" in Viruses, 15, no. 3 (2023):628,
https://doi.org/10.3390/v15030628 . .

TY  - DATA
AU  - Obradović, Mina
AU  - Malešević, Milka
AU  - Di Luca, Mariagrazia
AU  - Kekić, Dušan
AU  - Gajić, Ina
AU  - McAuliffe, Olivia
AU  - Neve, Horst
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2023
UR  - https://www.mdpi.com/1999-4915/15/3/628
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2065
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2072
T2  - Viruses
T1  - Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2072
ER  - 

@misc{
author = "Obradović, Mina and Malešević, Milka and Di Luca, Mariagrazia and Kekić, Dušan and Gajić, Ina and McAuliffe, Olivia and Neve, Horst and Stanisavljević, Nemanja and Vukotić, Goran and Kojić, Milan",
year = "2023",
journal = "Viruses",
title = "Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2072"
}

Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628. in Viruses.
https://hdl.handle.net/21.15107/rcub_imagine_2072

Obradović M, Malešević M, Di Luca M, Kekić D, Gajić I, McAuliffe O, Neve H, Stanisavljević N, Vukotić G, Kojić M. Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628. in Viruses. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2072 .

Obradović, Mina, Malešević, Milka, Di Luca, Mariagrazia, Kekić, Dušan, Gajić, Ina, McAuliffe, Olivia, Neve, Horst, Stanisavljević, Nemanja, Vukotić, Goran, Kojić, Milan, "Supplementary data for the article: Obradović, M., Malešević, M., Di Luca, M., Kekić, D., Gajić, I., McAuliffe, O., Neve, H., Stanisavljević, N., Vukotić, G.,& Kojić, M.. (2023). Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae. in Viruses, 15(3), 628. https://doi.org/10.3390/v15030628" in Viruses (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2072 .

TY  - CONF
AU  - Gardijan, Lazar
AU  - Kojić, Milan
AU  - Jovanović, Goran
AU  - Malešević, Milka
PY  - 2023
UR  - http://intor.torlakinstitut.com/handle/123456789/804
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2125
AB  - Introduction: Lactolisterin BU (LBU) is a potent bacteriocin derived from Lactococcuslactis subsp. lactisbv. diacetylactis BGBU1-4. It exhibits antimicrobial properties against Gram-positive food spoilage andfoodborne pathogens. This research aimed to explore the impact of amino acid substitution in LBU onits antimicrobial activity by utilizing in silico prediction of LBU’ssecondary structure and amino acid substitutions.Methods: The secondary structure of LBU was predicted using Phyre2 software. Five variants of LBUwere selected and chemically synthesized, along with unaltered LBU and BHT-B,serving as controls. Peptides were twofold diluted in distilled water, resulting in final concentrations ranging from 1000 µg/mlto 0.5 µg/ml. An agarspot test, employing 5 µl of the dilution, was conducted on three indicatorstrains:Lactococcus lactis BGMN1-596, Listeria monocytogenes ATCC19111, and Staphylococcus aureusATCC25923. The presence of inhibition zones was analyzed after overnight incubation at 37°C (S. aureus)and 30°C (L. lactis and L. monocytogenes).Results: Phyre2 analysis unveiled the presence of two α-helices in LBU’s structure. The majority of LBUvariants displayed altered antimicrobial activity, with some changes being genusspecific, potentially attributable to variances in cell wall composition. Some variants completely lost their activity, underscoring the significance of native amino acids or their physicochemical properties in the correspondingpositions within LBU’s structure. Furthermore, it was confirmed that chemically synthesized LBU effectively retains its antimicrobial activity.Conclusion: Changesin amino acid composition give insight on structure-function relationship of LBU.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Influence of amino acid substitution on the antimicrobial activity of bacteriocin lactolisterin BU
EP  - 123
SP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2125
ER  - 

@conference{
author = "Gardijan, Lazar and Kojić, Milan and Jovanović, Goran and Malešević, Milka",
year = "2023",
abstract = "Introduction: Lactolisterin BU (LBU) is a potent bacteriocin derived from Lactococcuslactis subsp. lactisbv. diacetylactis BGBU1-4. It exhibits antimicrobial properties against Gram-positive food spoilage andfoodborne pathogens. This research aimed to explore the impact of amino acid substitution in LBU onits antimicrobial activity by utilizing in silico prediction of LBU’ssecondary structure and amino acid substitutions.Methods: The secondary structure of LBU was predicted using Phyre2 software. Five variants of LBUwere selected and chemically synthesized, along with unaltered LBU and BHT-B,serving as controls. Peptides were twofold diluted in distilled water, resulting in final concentrations ranging from 1000 µg/mlto 0.5 µg/ml. An agarspot test, employing 5 µl of the dilution, was conducted on three indicatorstrains:Lactococcus lactis BGMN1-596, Listeria monocytogenes ATCC19111, and Staphylococcus aureusATCC25923. The presence of inhibition zones was analyzed after overnight incubation at 37°C (S. aureus)and 30°C (L. lactis and L. monocytogenes).Results: Phyre2 analysis unveiled the presence of two α-helices in LBU’s structure. The majority of LBUvariants displayed altered antimicrobial activity, with some changes being genusspecific, potentially attributable to variances in cell wall composition. Some variants completely lost their activity, underscoring the significance of native amino acids or their physicochemical properties in the correspondingpositions within LBU’s structure. Furthermore, it was confirmed that chemically synthesized LBU effectively retains its antimicrobial activity.Conclusion: Changesin amino acid composition give insight on structure-function relationship of LBU.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Influence of amino acid substitution on the antimicrobial activity of bacteriocin lactolisterin BU",
pages = "123-123",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2125"
}

Gardijan, L., Kojić, M., Jovanović, G.,& Malešević, M.. (2023). Influence of amino acid substitution on the antimicrobial activity of bacteriocin lactolisterin BU. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 123-123.
https://hdl.handle.net/21.15107/rcub_imagine_2125

Gardijan L, Kojić M, Jovanović G, Malešević M. Influence of amino acid substitution on the antimicrobial activity of bacteriocin lactolisterin BU. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:123-123.
https://hdl.handle.net/21.15107/rcub_imagine_2125 .

Gardijan, Lazar, Kojić, Milan, Jovanović, Goran, Malešević, Milka, "Influence of amino acid substitution on the antimicrobial activity of bacteriocin lactolisterin BU" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):123-123,
https://hdl.handle.net/21.15107/rcub_imagine_2125 .

TY  - JOUR
AU  - Dobrijević, Zoran
AU  - Karanović, Jelena
AU  - Savić-Pavićević, Dušanka
AU  - Brajušković, Goran
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2211
AB  - Dobrijević Z., J. Karanović, D. Savić-Pavićević, G. Brajušković (2023). The effect of epistatic interactions between genetic variants located in microRNA and silencing complex genes on prostate cancer progression risk.- Genetika, Vol 55, No.1, 263-275.
Previous studies conducted in Asian and European populations have provided evidence of the association between microRNA-related genetic variants and prostate cancer (PCa) risk and/or progression. Nevertheless, the results obtained in these studies are inconsistent, which could be explained by the limitations of single-locus main effect evaluations to detect joint effects of multiple genetic variants, reflected in statistical epistases. Therefore, we conducted the analysis of potential epistatic interactions between variants located in microRNA genes and in genes encoding the components of RNA-induced silencing complex (RISC) in relation with PCa risk/aggressiveness. Raw data on genotyping results from our previous studies involving four microRNA polymorphisms and five variants in RISC genes were subjected to the exclusion of samples based on missing data criterion, followed by the re-evaluation of Hardy-Weinberg equilibrium. Afterwards, these genotyping results were included in the Multifactor dimensionality reduction (MDR) analysis. Permutation testing was conducted in order to assess statistical significance of the best models from MDR tests. MDR tests on the risk of developing PCa yielded statistically insignificant results. Nevertheless, the MDR results for comparison of PCa patients with high and low cancer progression risk were statistically significant for the analysis that included rs11614913, with the 3-locus best model comprising this genetic variant, rs7813 and rs784567. We conclude that statistical epistasis between rs11614913 in hsa-miR-196a2, rs7813 in GEMIN4 and rs784567 in TARBP2 shows association with the invasiveness of PCa.
AB  - Ranije studije u evropskim i azijskim populacijama ukazale su na značajnu asocijaciju genetičkih varijanti sa efektom na funkciju mikroRNK sa rizikom za razvoj i/ili progresiju karcinoma prostate (KP). Međutim, rezultati navedenih studija su nepodudarni, za šta je jedan od mogućih razloga nemogućnost pristupa baziranih na proceni pojedinačnih efekata genetičkih varijanti da detektuju značajne zajedničke efekte više varijanti, a koji se reflektuju u statističke epistaze. Iz tog razloga, cilj naše studije bila je analiza potencijalnih epistatičkih interakcija između varijanti lociranih u genima za mikroRNK molekule i u genima za komponente utišavajućeg kompleksa indukovanog sa RNK (RISC) kao faktora rizika za razvoj i/ili progresiju KP. Rezultati genotipizacije dobijeni tokom sprovođenja naših ranijih studija, a koji uključuju podatke za četiri varijante u genima za mikroRNK i pet u genima za komponente RICS, podvrgnuti su inicijalnoj obradi podataka u smislu isključivanja uzoraka sa nedostajućim rezultatima, nakon čega je procenjeno odstupanje od Hardi-Vajnbergove ravnoteže. Rezultati su zatim analizirani metodom redukcije dimenzionalnosti višestrukih faktora (Multifactor dimensionality reduction - MDR analysis). Permutacioni testovi su sprovedeni sa ciljem procene statističke značajnosti najboljih modela iz MDR analize. Dobijeni rezultati MDR testova koji su se odnosili na rizik za razvoj KP nisu bili statistički značajni. S druge strane, MDR rezultati koji se odnose na rizik za progresiju KP bili su značajni za model koji uključuje tri lokusa: rs11614913, rs7813 and rs784567. Stoga, zaključci naše studije ukazuju na značaj epistatičkih interakcija između varijanti rs11614913 u hsa-miR-196a2, rs7813 u GEMIN4 i rs784567 u TARBP2 kao faktora koji ispoljavaju efekat na invazivnost KP.
PB  - Drustvo Geneticara Srbije
T2  - Genetika
T1  - The effect of epistatic interactions between genetic variants located in microrna and silencing complex genes on prostate cancer progression risk
T1  - Efekat epistatičkih interakcija između varijanti u genima za mikrornk i genima za proteine utišavajućeg kompleksa na rizik za razvoj i progresiju karcinoma prostate
EP  - 275
IS  - 1
SP  - 263
VL  - 55
DO  - 10.2298/GENSR23010263D
ER  - 

@article{
author = "Dobrijević, Zoran and Karanović, Jelena and Savić-Pavićević, Dušanka and Brajušković, Goran",
year = "2023",
abstract = "Dobrijević Z., J. Karanović, D. Savić-Pavićević, G. Brajušković (2023). The effect of epistatic interactions between genetic variants located in microRNA and silencing complex genes on prostate cancer progression risk.- Genetika, Vol 55, No.1, 263-275.
Previous studies conducted in Asian and European populations have provided evidence of the association between microRNA-related genetic variants and prostate cancer (PCa) risk and/or progression. Nevertheless, the results obtained in these studies are inconsistent, which could be explained by the limitations of single-locus main effect evaluations to detect joint effects of multiple genetic variants, reflected in statistical epistases. Therefore, we conducted the analysis of potential epistatic interactions between variants located in microRNA genes and in genes encoding the components of RNA-induced silencing complex (RISC) in relation with PCa risk/aggressiveness. Raw data on genotyping results from our previous studies involving four microRNA polymorphisms and five variants in RISC genes were subjected to the exclusion of samples based on missing data criterion, followed by the re-evaluation of Hardy-Weinberg equilibrium. Afterwards, these genotyping results were included in the Multifactor dimensionality reduction (MDR) analysis. Permutation testing was conducted in order to assess statistical significance of the best models from MDR tests. MDR tests on the risk of developing PCa yielded statistically insignificant results. Nevertheless, the MDR results for comparison of PCa patients with high and low cancer progression risk were statistically significant for the analysis that included rs11614913, with the 3-locus best model comprising this genetic variant, rs7813 and rs784567. We conclude that statistical epistasis between rs11614913 in hsa-miR-196a2, rs7813 in GEMIN4 and rs784567 in TARBP2 shows association with the invasiveness of PCa., Ranije studije u evropskim i azijskim populacijama ukazale su na značajnu asocijaciju genetičkih varijanti sa efektom na funkciju mikroRNK sa rizikom za razvoj i/ili progresiju karcinoma prostate (KP). Međutim, rezultati navedenih studija su nepodudarni, za šta je jedan od mogućih razloga nemogućnost pristupa baziranih na proceni pojedinačnih efekata genetičkih varijanti da detektuju značajne zajedničke efekte više varijanti, a koji se reflektuju u statističke epistaze. Iz tog razloga, cilj naše studije bila je analiza potencijalnih epistatičkih interakcija između varijanti lociranih u genima za mikroRNK molekule i u genima za komponente utišavajućeg kompleksa indukovanog sa RNK (RISC) kao faktora rizika za razvoj i/ili progresiju KP. Rezultati genotipizacije dobijeni tokom sprovođenja naših ranijih studija, a koji uključuju podatke za četiri varijante u genima za mikroRNK i pet u genima za komponente RICS, podvrgnuti su inicijalnoj obradi podataka u smislu isključivanja uzoraka sa nedostajućim rezultatima, nakon čega je procenjeno odstupanje od Hardi-Vajnbergove ravnoteže. Rezultati su zatim analizirani metodom redukcije dimenzionalnosti višestrukih faktora (Multifactor dimensionality reduction - MDR analysis). Permutacioni testovi su sprovedeni sa ciljem procene statističke značajnosti najboljih modela iz MDR analize. Dobijeni rezultati MDR testova koji su se odnosili na rizik za razvoj KP nisu bili statistički značajni. S druge strane, MDR rezultati koji se odnose na rizik za progresiju KP bili su značajni za model koji uključuje tri lokusa: rs11614913, rs7813 and rs784567. Stoga, zaključci naše studije ukazuju na značaj epistatičkih interakcija između varijanti rs11614913 u hsa-miR-196a2, rs7813 u GEMIN4 i rs784567 u TARBP2 kao faktora koji ispoljavaju efekat na invazivnost KP.",
publisher = "Drustvo Geneticara Srbije",
journal = "Genetika",
title = "The effect of epistatic interactions between genetic variants located in microrna and silencing complex genes on prostate cancer progression risk, Efekat epistatičkih interakcija između varijanti u genima za mikrornk i genima za proteine utišavajućeg kompleksa na rizik za razvoj i progresiju karcinoma prostate",
pages = "275-263",
number = "1",
volume = "55",
doi = "10.2298/GENSR23010263D"
}

Dobrijević, Z., Karanović, J., Savić-Pavićević, D.,& Brajušković, G.. (2023). The effect of epistatic interactions between genetic variants located in microrna and silencing complex genes on prostate cancer progression risk. in Genetika
Drustvo Geneticara Srbije., 55(1), 263-275.
https://doi.org/10.2298/GENSR23010263D

Dobrijević Z, Karanović J, Savić-Pavićević D, Brajušković G. The effect of epistatic interactions between genetic variants located in microrna and silencing complex genes on prostate cancer progression risk. in Genetika. 2023;55(1):263-275.
doi:10.2298/GENSR23010263D .

Dobrijević, Zoran, Karanović, Jelena, Savić-Pavićević, Dušanka, Brajušković, Goran, "The effect of epistatic interactions between genetic variants located in microrna and silencing complex genes on prostate cancer progression risk" in Genetika, 55, no. 1 (2023):263-275,
https://doi.org/10.2298/GENSR23010263D . .

TY  - CHAP
AU  - Zeljić, Katarina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1825
AB  - Otkrivanje velikog sveta nekodirajućih RNK molekula i njihove regulatorne funkcije je rezultovalo
značajnim porastom broja publikacija i interesovanja istraživača za ovu oblast. Posebno su brojna
istraživanja jedne od klasa malih nekodirajućih RNK, mikroRNK (miRNK), kao biomarkera za
predikciju, dijagnozu i prognozu različitih humanih patologija. Razvoj i primena bioinformatičkih
alatki za analizu miRNK koje su slobodno dostupne na internetu su omogućile sprovođenje
funkcionalnih analiza miRNK, njihovih interakcija i asocijacija sa humanim bolestima. Primenom tzv.
in silico softvera za miRNK analizu istraživačima je omogućeno relativno jednostavno izvođenje zaključaka
i dalje preciznije usmeravanje toka istraživanja. U poglavlju će biti dat pregled nekoliko
odabranih i besplatno dostupnih bioinformatičkih alata čije korišćenje ne zahteva poznavanje programiranja,
a koje omogućavaju sprovođenje detaljnih analiza miRNK, funkcionalnih anotacija i asocijacija
sa humanim bolestima.
AB  - The discovery of a large world of non-coding RNA molecules and their regulatory functions has
resulted in a significant increase in publications and research interest in this field. There are numerous
studies of one of the classes of small non-coding RNA, microRNA (miRNA) as a biomarker for the
prediction, diagnosis and prognosis of various human pathologies. The development and application
of bioinformatics tools for miRNA analysis that are freely available on the internet have enabled
the implementation of functional analyzes of miRNAs, their interactions and associations with human
diseases. Applying the so-called in silico software for miRNA analysis allowed researchers to relatively
easily draw conclusions and precisely direct the flow of research. The chapter will provide an overview
of several selected and freely available bioinformatics tools whose use does not require knowledge
of programming, and which allow detailed analysis of miRNAs, functional annotations and associations
with human diseases.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Bioinformatički alati za analizu mikroRNK
T1  - Bioinformatics tools for the analysis of microRNA
EP  - 274
SP  - 255
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1825
ER  - 

@inbook{
author = "Zeljić, Katarina",
year = "2022",
abstract = "Otkrivanje velikog sveta nekodirajućih RNK molekula i njihove regulatorne funkcije je rezultovalo
značajnim porastom broja publikacija i interesovanja istraživača za ovu oblast. Posebno su brojna
istraživanja jedne od klasa malih nekodirajućih RNK, mikroRNK (miRNK), kao biomarkera za
predikciju, dijagnozu i prognozu različitih humanih patologija. Razvoj i primena bioinformatičkih
alatki za analizu miRNK koje su slobodno dostupne na internetu su omogućile sprovođenje
funkcionalnih analiza miRNK, njihovih interakcija i asocijacija sa humanim bolestima. Primenom tzv.
in silico softvera za miRNK analizu istraživačima je omogućeno relativno jednostavno izvođenje zaključaka
i dalje preciznije usmeravanje toka istraživanja. U poglavlju će biti dat pregled nekoliko
odabranih i besplatno dostupnih bioinformatičkih alata čije korišćenje ne zahteva poznavanje programiranja,
a koje omogućavaju sprovođenje detaljnih analiza miRNK, funkcionalnih anotacija i asocijacija
sa humanim bolestima., The discovery of a large world of non-coding RNA molecules and their regulatory functions has
resulted in a significant increase in publications and research interest in this field. There are numerous
studies of one of the classes of small non-coding RNA, microRNA (miRNA) as a biomarker for the
prediction, diagnosis and prognosis of various human pathologies. The development and application
of bioinformatics tools for miRNA analysis that are freely available on the internet have enabled
the implementation of functional analyzes of miRNAs, their interactions and associations with human
diseases. Applying the so-called in silico software for miRNA analysis allowed researchers to relatively
easily draw conclusions and precisely direct the flow of research. The chapter will provide an overview
of several selected and freely available bioinformatics tools whose use does not require knowledge
of programming, and which allow detailed analysis of miRNAs, functional annotations and associations
with human diseases.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Bioinformatički alati za analizu mikroRNK, Bioinformatics tools for the analysis of microRNA",
pages = "274-255",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1825"
}

Zeljić, K.. (2022). Bioinformatički alati za analizu mikroRNK. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo., 2, 255-274.
https://hdl.handle.net/21.15107/rcub_imagine_1825

Zeljić K. Bioinformatički alati za analizu mikroRNK. in Trendovi u molekularnoj Biologiji. 2022;2:255-274.
https://hdl.handle.net/21.15107/rcub_imagine_1825 .

Zeljić, Katarina, "Bioinformatički alati za analizu mikroRNK" in Trendovi u molekularnoj Biologiji, 2 (2022):255-274,
https://hdl.handle.net/21.15107/rcub_imagine_1825 .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Gardijan, Lazar
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1525
AB  - Signal transduction systems are the key players of bacterial adaptation and survival. The orthodox two-component signal transduction systems perceive diverse environmental stimuli and their regulatory response leads to cellular changes. Although rarely described, the unorthodox three-component systems are also implemented in the regulation of major bacterial behavior such as the virulence of clinically relevant pathogen P. aeruginosa. Previously, we described a novel three-component system in P. capeferrum WCS358 (RclSAR) where the sensor kinase RclS stimulates the intI1 transcription in stationary growth phase. In this study, using rclS knock-out mutant, we identified RclSAR regulon in P. capeferrum WCS358. The RNA sequencing revealed that activity of RclSAR signal transduction system is growth phase dependent with more pronounced regulatory potential in early stages of growth. Transcriptional analysis emphasized the role of RclSAR in global regulation and indicated the involvement of this system in regulation of diverse cellular activities such as RNA binding and metabolic and biocontrol processes. Importantly, phenotypic comparison of WCS358 wild type and Delta rclS mutant showed that RclS sensor kinase contributes to modulation of antibiotic resistance, production of AHLs and siderophore as well as host cell adherence and cytotoxicity. Finally, we proposed the improved model of interplay between RclSAR, RpoS and LasIR regulatory systems in P. capeferrum WCS358.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum
IS  - 15
VL  - 23
DO  - 10.3390/ijms23158232
ER  - 

@article{
author = "Novović, Katarina and Malešević, Milka and Dinić, Miroslav and Gardijan, Lazar and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "Signal transduction systems are the key players of bacterial adaptation and survival. The orthodox two-component signal transduction systems perceive diverse environmental stimuli and their regulatory response leads to cellular changes. Although rarely described, the unorthodox three-component systems are also implemented in the regulation of major bacterial behavior such as the virulence of clinically relevant pathogen P. aeruginosa. Previously, we described a novel three-component system in P. capeferrum WCS358 (RclSAR) where the sensor kinase RclS stimulates the intI1 transcription in stationary growth phase. In this study, using rclS knock-out mutant, we identified RclSAR regulon in P. capeferrum WCS358. The RNA sequencing revealed that activity of RclSAR signal transduction system is growth phase dependent with more pronounced regulatory potential in early stages of growth. Transcriptional analysis emphasized the role of RclSAR in global regulation and indicated the involvement of this system in regulation of diverse cellular activities such as RNA binding and metabolic and biocontrol processes. Importantly, phenotypic comparison of WCS358 wild type and Delta rclS mutant showed that RclS sensor kinase contributes to modulation of antibiotic resistance, production of AHLs and siderophore as well as host cell adherence and cytotoxicity. Finally, we proposed the improved model of interplay between RclSAR, RpoS and LasIR regulatory systems in P. capeferrum WCS358.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum",
number = "15",
volume = "23",
doi = "10.3390/ijms23158232"
}

Novović, K., Malešević, M., Dinić, M., Gardijan, L., Kojić, M.,& Jovčić, B.. (2022). RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum. in International Journal of Molecular Sciences
MDPI, Basel., 23(15).
https://doi.org/10.3390/ijms23158232

Novović K, Malešević M, Dinić M, Gardijan L, Kojić M, Jovčić B. RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum. in International Journal of Molecular Sciences. 2022;23(15).
doi:10.3390/ijms23158232 .

Novović, Katarina, Malešević, Milka, Dinić, Miroslav, Gardijan, Lazar, Kojić, Milan, Jovčić, Branko, "RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum" in International Journal of Molecular Sciences, 23, no. 15 (2022),
https://doi.org/10.3390/ijms23158232 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Stojković, Dejan
AU  - Jovčić, Branko
AU  - Soković, Marina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1566
AB  - The rising incidence of antibiotic resistant microorganisms urges novel antimicrobials development with polyphenols as appealing potential therapeutics. We aimed to reveal the most promising polyphenols among hesperetin, hesperidin, naringenin, naringin, taxifolin, rutin, isoquercitrin, morin, chlorogenic acid, ferulic acid, p-coumaric acid, and gallic acid based on antimicrobial capacity, antibiofilm potential, and lack of cytotoxicity towards HaCaT, and to further test its antivirulence mechanisms. Although the majority of studied polyphenols were able to inhibit bacterial growth and biofilm formation, the most promising activities were observed for rutin. Further investigation proved rutin's ability to prevent/eradicate Pseudomonas aeruginosa and MRSA urinary catheter biofilms. Besides reduction of biofilm biomass, rutin antibiofilm mechanisms included reduction of cell viability, exopolysaccharide, and extracellular DNA levels. Moderate reduction of bacterial adhesion to human keratinocytes upon treatment was observed. Rutin antivirulence mechanisms included an impact on P. aeruginosa protease, pyocyanin, rhamnolipid, and elastase production and the downregulation of the lasI, lasR, rhlI, rhlR, pqsA and mvfR genes. Rutin also interfered with membrane permeability. Polyphenols could repress antibiotic resistant bacteria. Rutin has shown wide antimicrobial and antibiofilm capacity employing a range of mechanisms that might be used for the development of novel antimicrobials.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence
IS  - 3
VL  - 15
DO  - 10.3390/ph15030385
ER  - 

@article{
author = "Ivanov, Marija and Novović, Katarina and Malešević, Milka and Dinić, Miroslav and Stojković, Dejan and Jovčić, Branko and Soković, Marina",
year = "2022",
abstract = "The rising incidence of antibiotic resistant microorganisms urges novel antimicrobials development with polyphenols as appealing potential therapeutics. We aimed to reveal the most promising polyphenols among hesperetin, hesperidin, naringenin, naringin, taxifolin, rutin, isoquercitrin, morin, chlorogenic acid, ferulic acid, p-coumaric acid, and gallic acid based on antimicrobial capacity, antibiofilm potential, and lack of cytotoxicity towards HaCaT, and to further test its antivirulence mechanisms. Although the majority of studied polyphenols were able to inhibit bacterial growth and biofilm formation, the most promising activities were observed for rutin. Further investigation proved rutin's ability to prevent/eradicate Pseudomonas aeruginosa and MRSA urinary catheter biofilms. Besides reduction of biofilm biomass, rutin antibiofilm mechanisms included reduction of cell viability, exopolysaccharide, and extracellular DNA levels. Moderate reduction of bacterial adhesion to human keratinocytes upon treatment was observed. Rutin antivirulence mechanisms included an impact on P. aeruginosa protease, pyocyanin, rhamnolipid, and elastase production and the downregulation of the lasI, lasR, rhlI, rhlR, pqsA and mvfR genes. Rutin also interfered with membrane permeability. Polyphenols could repress antibiotic resistant bacteria. Rutin has shown wide antimicrobial and antibiofilm capacity employing a range of mechanisms that might be used for the development of novel antimicrobials.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence",
number = "3",
volume = "15",
doi = "10.3390/ph15030385"
}

Ivanov, M., Novović, K., Malešević, M., Dinić, M., Stojković, D., Jovčić, B.,& Soković, M.. (2022). Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence. in Pharmaceuticals
MDPI, Basel., 15(3).
https://doi.org/10.3390/ph15030385

Ivanov M, Novović K, Malešević M, Dinić M, Stojković D, Jovčić B, Soković M. Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence. in Pharmaceuticals. 2022;15(3).
doi:10.3390/ph15030385 .

Ivanov, Marija, Novović, Katarina, Malešević, Milka, Dinić, Miroslav, Stojković, Dejan, Jovčić, Branko, Soković, Marina, "Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence" in Pharmaceuticals, 15, no. 3 (2022),
https://doi.org/10.3390/ph15030385 . .

TY  - CHAP
AU  - Dakić, Tamara
AU  - Vujović, Predrag
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1819
AB  - Nakon njegovog otkrića početkom dvadesetog veka, dugo je smatrano da se insulin isključivo sintetiše
u pankreasu i da u centralni nervni sistem (CNS) dospeva prolaskom kroz krvno-moždanu barijeru.
Međutim, razvojem molekularno-bioloških metoda potvrđeno je prisustvo iRNK za preporoinsulin, samog
(prepro)insulina, kao i C-peptida u nekim regionima mozga. Time je višestruko potvrđeno da se insulin,
osim u pankreasu, sintetiše i u CNS. Nakon otkrića da se i receptor za insulin eksprimira u CNS, ispitivanja
su bila usmerena ka upoznavanju dejstva ovog hormona u mozgu. Pokazano je da je, između ostalog,
uključen u kontrolu apetita, regulaciji reprodukcije, ali i procesa vezanih za učenje i konsolidaciju memorije.
Takođe je utvrđeno da su poremećaji dejstva insulina u CNS povezani sa razvojem nekih neurodegenerativnih
bolesti.
Iako je većina pomenutih efekata prvobitno pripisana insulinu koji iz sistemske cirkulacije dospeva u
CNS, sve više se dizajniraju studije koje su orijentisane isključivo ka upoznavaju funkcije insulina koji nastaje
u mozgu. Do sada je pokazano da se produkcija insulina u hipotalamusu smanjuje tokom stresnih situacija,
što se negativno odražava na sintezu hormona rasta u adenohipofizi i njegovu sekreciju u sistemsku
cirkulaciju. Nasuprot tome, pokazano je da kratkotrajno gladovanje povećava ekspresiju insulina u neuronima
istog moždanog regiona ali i da ovaj fenomen nije povezan sa povećanjem stope ugradnje glukoznih
transportera u ćelijsku membranu, niti sinteze glikogena u astrocitima. Biće potrebno sprovesti
dodatne studije kako bi se u većoj meri upoznala uloga insulina koji nastaje unutar CNS.
AB  - After being discovered in the early twentieth century, insulin was long considered to be exclusively
produced in the β-pancreatic cells. Therefore the presence of this hormone in the central nervous system
(CNS) was accounted for solely by its transport across the blood-brain barrier. However, owing to the advances
in molecular biological techniques, it was established that the preproinsulin mRNA, the (pre)prounsulin
peptide and C-peptide were also present in the CNS parenchyma. These findings unequivocally proved
that, in addition to the pancreas, insulin was also produced in the CNS. After discovery that the insulin receptor
was also expressed in the brain, numerous studies aimed to discover the insulin actions within the
CNS. It was found that insulin was involved in the control of appetite, reproduction, learning and memory
consolidation. Moreover, it was confirmed that the impairment of insulin signaling within the certain brain
regions was associated with various neurodegenerative disorders. Although these effects were initially assigned
to the action of the insulin transported from the circulation into the CNS, more studies were designed
to uncover the roles of insulin produced in the CNS. It has been shown so far that stress decreases
hypothalamic insulin production which subsequently results in the decreased growth hormone production
and its secretion form adenohypophysis. Furthermore, short-term fasting increased hypothalamic insulin
expression in rats, albeit this phenomenon did not result in either increased glucose uptake nor
glycogen production in this brain region. More studies are needed to fully elucidate the role of the centrally
produced insulin.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Ekspresija i funkcija insulina u centralnom nervnom sistemu
T1  - Insulin expression and action in the central nervous system
EP  - 167
IS  - 2
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1819
ER  - 

@inbook{
author = "Dakić, Tamara and Vujović, Predrag",
year = "2022",
abstract = "Nakon njegovog otkrića početkom dvadesetog veka, dugo je smatrano da se insulin isključivo sintetiše
u pankreasu i da u centralni nervni sistem (CNS) dospeva prolaskom kroz krvno-moždanu barijeru.
Međutim, razvojem molekularno-bioloških metoda potvrđeno je prisustvo iRNK za preporoinsulin, samog
(prepro)insulina, kao i C-peptida u nekim regionima mozga. Time je višestruko potvrđeno da se insulin,
osim u pankreasu, sintetiše i u CNS. Nakon otkrića da se i receptor za insulin eksprimira u CNS, ispitivanja
su bila usmerena ka upoznavanju dejstva ovog hormona u mozgu. Pokazano je da je, između ostalog,
uključen u kontrolu apetita, regulaciji reprodukcije, ali i procesa vezanih za učenje i konsolidaciju memorije.
Takođe je utvrđeno da su poremećaji dejstva insulina u CNS povezani sa razvojem nekih neurodegenerativnih
bolesti.
Iako je većina pomenutih efekata prvobitno pripisana insulinu koji iz sistemske cirkulacije dospeva u
CNS, sve više se dizajniraju studije koje su orijentisane isključivo ka upoznavaju funkcije insulina koji nastaje
u mozgu. Do sada je pokazano da se produkcija insulina u hipotalamusu smanjuje tokom stresnih situacija,
što se negativno odražava na sintezu hormona rasta u adenohipofizi i njegovu sekreciju u sistemsku
cirkulaciju. Nasuprot tome, pokazano je da kratkotrajno gladovanje povećava ekspresiju insulina u neuronima
istog moždanog regiona ali i da ovaj fenomen nije povezan sa povećanjem stope ugradnje glukoznih
transportera u ćelijsku membranu, niti sinteze glikogena u astrocitima. Biće potrebno sprovesti
dodatne studije kako bi se u većoj meri upoznala uloga insulina koji nastaje unutar CNS., After being discovered in the early twentieth century, insulin was long considered to be exclusively
produced in the β-pancreatic cells. Therefore the presence of this hormone in the central nervous system
(CNS) was accounted for solely by its transport across the blood-brain barrier. However, owing to the advances
in molecular biological techniques, it was established that the preproinsulin mRNA, the (pre)prounsulin
peptide and C-peptide were also present in the CNS parenchyma. These findings unequivocally proved
that, in addition to the pancreas, insulin was also produced in the CNS. After discovery that the insulin receptor
was also expressed in the brain, numerous studies aimed to discover the insulin actions within the
CNS. It was found that insulin was involved in the control of appetite, reproduction, learning and memory
consolidation. Moreover, it was confirmed that the impairment of insulin signaling within the certain brain
regions was associated with various neurodegenerative disorders. Although these effects were initially assigned
to the action of the insulin transported from the circulation into the CNS, more studies were designed
to uncover the roles of insulin produced in the CNS. It has been shown so far that stress decreases
hypothalamic insulin production which subsequently results in the decreased growth hormone production
and its secretion form adenohypophysis. Furthermore, short-term fasting increased hypothalamic insulin
expression in rats, albeit this phenomenon did not result in either increased glucose uptake nor
glycogen production in this brain region. More studies are needed to fully elucidate the role of the centrally
produced insulin.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Ekspresija i funkcija insulina u centralnom nervnom sistemu, Insulin expression and action in the central nervous system",
pages = "167-155",
number = "2",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1819"
}

Dakić, T.,& Vujović, P.. (2022). Ekspresija i funkcija insulina u centralnom nervnom sistemu. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(2), 155-167.
https://hdl.handle.net/21.15107/rcub_imagine_1819

Dakić T, Vujović P. Ekspresija i funkcija insulina u centralnom nervnom sistemu. in Trendovi u molekularnoj Biologiji. 2022;(2):155-167.
https://hdl.handle.net/21.15107/rcub_imagine_1819 .

Dakić, Tamara, Vujović, Predrag, "Ekspresija i funkcija insulina u centralnom nervnom sistemu" in Trendovi u molekularnoj Biologiji, no. 2 (2022):155-167,
https://hdl.handle.net/21.15107/rcub_imagine_1819 .

TY  - CONF
AU  - Radmilović, Mihajlo
AU  - Drvenica, Ivana
AU  - Rabasović, Mihailo
AU  - Ilić, Vesna
AU  - Pavlović, Danica
AU  - Oasa, Sho
AU  - Vukojević, Vladana
AU  - Perić, Mina
AU  - Krmpot, Aleksandar
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1730
AB  - Oslikavanje eritrocita bez obeležavanja pomoću konfokalne ili
epifluorescentne mikroskopije je praktično nemoguće jer se fluorescencija od
hemoglobina (Hb) ne može detektovati zbog brzih neradijativnih raspada koji su
dominantni u odnosu na spontanu emisiju. Sa druge strane, demonstrirana je
značajna dvofotonska apsorptivnost Hb u infracrvenoj oblasti1 kao i
dvofotonski pobuđena fluorescencija (TPEF) od novoformiranog fotoprodukta2
nakon interakcije ultrakratkih laserskih impulsa sa Hb. TPEF mikroskopija je
korišćena u brojnim studijama za oslikavanje Hb i eritrocita bez obeležavanja.3
U ovom radu prikazane su fotofizičke osobine i potencijalne primene
novoformiranog fotoprodukta. Kreirane su fluorescentne mikrostrukture
pomoću direktnog laserskog upisivanja na tankim Hb slojevima (micropatterning).
Fotoprodukt je okarakterisan pomoću različitih (mikro)spektroskopskih
tehnika: emisije nakon dvofotonske apsorpcije, UV-VIS apsorpcije i spektralnog
oslikavanja. Razmatrane su spektroskopske osobine protoporfirina IX (PpIX)
pošto je on strukturni deo Hb i potencijalno može biti prekursor za formiranje
fotoprodukta. Takođe, Hb je tretiran vodonik peroksidom da bi se uočila
optička i spektroskopska sličnost tako dobijenog jedinjenja i fotoprodukta.
Potvrđena je dugovremena fotostabilnost fotoprodukta u periodima od nekoliko
meseci. Na osnovu toga što je fotoprodukt moguće dobiti na prostorno i
vremenski kontrolisan način, izvršeno je fotoobeležavanje i praćenje kretanja
pojedinačnih eritrocita u humanoj punoj krvi zdravog donora.
AB  - Осликавање еритроцита без обележавања помоћу конфокалне или
епифлуоресцентне микроскопије је практично немогуће јер се флуоресценција од
хемоглобина (Хб) не може детектовати због брзих нерадијативних распада који су
доминантни у односу на спонтану емисију. Са друге стране, демонстрирана је
значајна двофотонска апсорптивност Хб у инфрацрвеној области1 као и
двофотонски побуђена флуоресценција (ТPEF) од новоформираног фотопродукта2
након интеракције ултракратких ласерских импулса са Хб. ТPEF микроскопија је
коришћена у бројним студијама за осликавање Хб и еритроцита без обележавања.3
У овом раду приказане су фотофизичке особине и потенцијалне примене
новоформираног фотопродукта. Креиране су флуоресцентне микроструктуре
помоћу директног ласерског уписивања на танким Хб слојевима (micropatterning).
Фотопродукт је окарактерисан помоћу различитих (микро)спектроскопских
техника: емисије након двофотонске апсорпције, UV-VIS апсорпције и спектралног
осликавања. Разматране су спектроскопске особине протопорфирина IX (PpIX)
пошто је он структурни део Хб и потенцијално може бити прекурсор за формирање
фотопродукта. Такође, Хб је третиран водоник пероксидом да би се уочила
оптичка и спектроскопска сличност тако добијеног једињења и фотопродукта.
Потврђена је дуговремена фотостабилност фотопродукта у периодима од неколико
месеци. На основу тога што је фотопродукт могуће добити на просторно и
временски контролисан начин, извршено је фотообележавање и праћење кретања
појединачних еритроцита у хуманој пуној крви здравог донора.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita
T1  - Нелинеарна ласерска скенирајућа микроскопија хемоглобина и еритроцита
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1730
ER  - 

@conference{
author = "Radmilović, Mihajlo and Drvenica, Ivana and Rabasović, Mihailo and Ilić, Vesna and Pavlović, Danica and Oasa, Sho and Vukojević, Vladana and Perić, Mina and Krmpot, Aleksandar",
year = "2022",
abstract = "Oslikavanje eritrocita bez obeležavanja pomoću konfokalne ili
epifluorescentne mikroskopije je praktično nemoguće jer se fluorescencija od
hemoglobina (Hb) ne može detektovati zbog brzih neradijativnih raspada koji su
dominantni u odnosu na spontanu emisiju. Sa druge strane, demonstrirana je
značajna dvofotonska apsorptivnost Hb u infracrvenoj oblasti1 kao i
dvofotonski pobuđena fluorescencija (TPEF) od novoformiranog fotoprodukta2
nakon interakcije ultrakratkih laserskih impulsa sa Hb. TPEF mikroskopija je
korišćena u brojnim studijama za oslikavanje Hb i eritrocita bez obeležavanja.3
U ovom radu prikazane su fotofizičke osobine i potencijalne primene
novoformiranog fotoprodukta. Kreirane su fluorescentne mikrostrukture
pomoću direktnog laserskog upisivanja na tankim Hb slojevima (micropatterning).
Fotoprodukt je okarakterisan pomoću različitih (mikro)spektroskopskih
tehnika: emisije nakon dvofotonske apsorpcije, UV-VIS apsorpcije i spektralnog
oslikavanja. Razmatrane su spektroskopske osobine protoporfirina IX (PpIX)
pošto je on strukturni deo Hb i potencijalno može biti prekursor za formiranje
fotoprodukta. Takođe, Hb je tretiran vodonik peroksidom da bi se uočila
optička i spektroskopska sličnost tako dobijenog jedinjenja i fotoprodukta.
Potvrđena je dugovremena fotostabilnost fotoprodukta u periodima od nekoliko
meseci. Na osnovu toga što je fotoprodukt moguće dobiti na prostorno i
vremenski kontrolisan način, izvršeno je fotoobeležavanje i praćenje kretanja
pojedinačnih eritrocita u humanoj punoj krvi zdravog donora., Осликавање еритроцита без обележавања помоћу конфокалне или
епифлуоресцентне микроскопије је практично немогуће јер се флуоресценција од
хемоглобина (Хб) не може детектовати због брзих нерадијативних распада који су
доминантни у односу на спонтану емисију. Са друге стране, демонстрирана је
значајна двофотонска апсорптивност Хб у инфрацрвеној области1 као и
двофотонски побуђена флуоресценција (ТPEF) од новоформираног фотопродукта2
након интеракције ултракратких ласерских импулса са Хб. ТPEF микроскопија је
коришћена у бројним студијама за осликавање Хб и еритроцита без обележавања.3
У овом раду приказане су фотофизичке особине и потенцијалне примене
новоформираног фотопродукта. Креиране су флуоресцентне микроструктуре
помоћу директног ласерског уписивања на танким Хб слојевима (micropatterning).
Фотопродукт је окарактерисан помоћу различитих (микро)спектроскопских
техника: емисије након двофотонске апсорпције, UV-VIS апсорпције и спектралног
осликавања. Разматране су спектроскопске особине протопорфирина IX (PpIX)
пошто је он структурни део Хб и потенцијално може бити прекурсор за формирање
фотопродукта. Такође, Хб је третиран водоник пероксидом да би се уочила
оптичка и спектроскопска сличност тако добијеног једињења и фотопродукта.
Потврђена је дуговремена фотостабилност фотопродукта у периодима од неколико
месеци. На основу тога што је фотопродукт могуће добити на просторно и
временски контролисан начин, извршено је фотообележавање и праћење кретања
појединачних еритроцита у хуманој пуној крви здравог донора.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita, Нелинеарна ласерска скенирајућа микроскопија хемоглобина и еритроцита",
pages = "19",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1730"
}

Radmilović, M., Drvenica, I., Rabasović, M., Ilić, V., Pavlović, D., Oasa, S., Vukojević, V., Perić, M.,& Krmpot, A.. (2022). Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 19.
https://hdl.handle.net/21.15107/rcub_imagine_1730

Radmilović M, Drvenica I, Rabasović M, Ilić V, Pavlović D, Oasa S, Vukojević V, Perić M, Krmpot A. Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita. in Treći kongres biologa Srbije. 2022;:19.
https://hdl.handle.net/21.15107/rcub_imagine_1730 .

Radmilović, Mihajlo, Drvenica, Ivana, Rabasović, Mihailo, Ilić, Vesna, Pavlović, Danica, Oasa, Sho, Vukojević, Vladana, Perić, Mina, Krmpot, Aleksandar, "Nelinearna laserska skenirajuća mikroskopija hemoglobina i eritrocita" in Treći kongres biologa Srbije (2022):19,
https://hdl.handle.net/21.15107/rcub_imagine_1730 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Ilić, Bojan
AU  - Radulović, Niko
AU  - Mladenović, Marko
AU  - Makarov, Slobodan
AU  - Divac Rankov, Aleksandra
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1731
AB  - Najveći broj stonoga iz klase Diplopoda poseduje hemijsku zaštitu od predatora
i/ili patogenih mikroorganizama, koji podrazumeva prisustvo odbrambenih
žlezda (ozadena) na trupu čiji se sekreti izbacuju u spoljašnju sredinu preko
otvora koji se naziva ozopora. Predstavnici reda Julida su poznati po tome da su
najčešće dominantne komponente njihovih odbrambenih sekreta hinoni. Pored
hinona, u sekretima ozadena Julida registrovani su i alkoholi, aldehidi, ketoni,
fenolna jedinjenja, kao i brojni estri zasićenih i nezasićenih karboksilnih
kiselina. Dosadašnja istraživanja su pokazala da su ekstrakti odbrambenih
sekreta Julida, kao i pojedinačna jedinjenja koja u njihov sastav ulaze, biološki
aktivni prirodni proizvodi, ali njihov citotoksični potencijal nije dovoljno
istražen. U ovoj studiji je ispitivan uticaj različitih estara linoleinske
kiseline (butil-, pentil-, heksil-, heptil-, oktil-, nonil-, fenetil- i 3-
fenilpropil-linoleat) detektovanih u odbrambenim sekretima stonoga
Megaphyllum bosniense i M. unilineatum na vijabilnost normalnih (BEAS-2B) i
kancerskih (A549) ćelija pluća korišćenjem MTT testa. Svi ispitivani estri su
smanjivali vijabilnost ćelija, pri čemu je postojala značajna razlika u odgovoru
kancerskih u odnosu na normalne ćelije u slučaju tri estra (heksil-, fenetil- i 3-
fenilpropil-linoleat). Iako su estri karboksilnih kiselina poznati kao malo
reaktivna jedinjenja, rezultati ove studije pokazuju da predstavnici ove klase
hemijskih jedinjenja mogu imati citotoksični potencijal.
AB  - Највећи број стонога из класе Diplopoda поседује хемијску заштиту од предатора
и/или патогених микроорганизама, који подразумева присуство одбрамбених
жлезда (озадена) на трупу чији се секрети избацују у спољашњу средину преко
отвора који се назива озопора. Представници реда Julida су познати по томе да су
најчешће доминантне компоненте њихових одбрамбених секрета хинони. Поред
хинона, у секретима озадена Julida регистровани су и алкохоли, алдехиди, кетони,
фенолна једињења, као и бројни естри засићених и незасићених карбоксилних
киселина. Досадашња истраживања су показала да су екстракти одбрамбених
секрета Julida, као и појединачна једињења која у њихов састав улазе, биолошки
активни природни производи, али њихов цитотоксични потенцијал није довољно
истражен. У овој студији је испитиван утицај различитих естара линолеинске
киселине (бутил-, пентил-, хексил-, хептил-, октил-, нонил-, фенетил- и 3-
фенилпропил-линолеат) детектованих у одбрамбеним секретима стонога
Megaphyllum bosniense и M. unilineatum на вијабилност нормалних (BEAS-2B) и
канцeрских (A549) ћелија плућа коришћењем МТТ теста. Сви испитивани естри су
смањивали вијабилност ћелија, при чему је постојала значајна разлика у одговору
канцерских у односу на нормалне ћелије у случају три естра (хексил-, фенетил- и 3-
фенилпропил-линолеат). Иако су естри карбоксилних киселина познати као мало
реактивна једињења, резултати ове студије показују да представници ове класе
хемијских једињења могу имати цитотоксични потенцијал.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida)
T1  - Цитотоксични потенцијал естара линолеинске киселине детектованих у одбрамбеним секретима стонога Megaphyllum bosniense и M. unilineatum (Diplopoda: Julida)
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1731
ER  - 

@conference{
author = "Milovanović, Jelena and Ilić, Bojan and Radulović, Niko and Mladenović, Marko and Makarov, Slobodan and Divac Rankov, Aleksandra",
year = "2022",
abstract = "Najveći broj stonoga iz klase Diplopoda poseduje hemijsku zaštitu od predatora
i/ili patogenih mikroorganizama, koji podrazumeva prisustvo odbrambenih
žlezda (ozadena) na trupu čiji se sekreti izbacuju u spoljašnju sredinu preko
otvora koji se naziva ozopora. Predstavnici reda Julida su poznati po tome da su
najčešće dominantne komponente njihovih odbrambenih sekreta hinoni. Pored
hinona, u sekretima ozadena Julida registrovani su i alkoholi, aldehidi, ketoni,
fenolna jedinjenja, kao i brojni estri zasićenih i nezasićenih karboksilnih
kiselina. Dosadašnja istraživanja su pokazala da su ekstrakti odbrambenih
sekreta Julida, kao i pojedinačna jedinjenja koja u njihov sastav ulaze, biološki
aktivni prirodni proizvodi, ali njihov citotoksični potencijal nije dovoljno
istražen. U ovoj studiji je ispitivan uticaj različitih estara linoleinske
kiseline (butil-, pentil-, heksil-, heptil-, oktil-, nonil-, fenetil- i 3-
fenilpropil-linoleat) detektovanih u odbrambenim sekretima stonoga
Megaphyllum bosniense i M. unilineatum na vijabilnost normalnih (BEAS-2B) i
kancerskih (A549) ćelija pluća korišćenjem MTT testa. Svi ispitivani estri su
smanjivali vijabilnost ćelija, pri čemu je postojala značajna razlika u odgovoru
kancerskih u odnosu na normalne ćelije u slučaju tri estra (heksil-, fenetil- i 3-
fenilpropil-linoleat). Iako su estri karboksilnih kiselina poznati kao malo
reaktivna jedinjenja, rezultati ove studije pokazuju da predstavnici ove klase
hemijskih jedinjenja mogu imati citotoksični potencijal., Највећи број стонога из класе Diplopoda поседује хемијску заштиту од предатора
и/или патогених микроорганизама, који подразумева присуство одбрамбених
жлезда (озадена) на трупу чији се секрети избацују у спољашњу средину преко
отвора који се назива озопора. Представници реда Julida су познати по томе да су
најчешће доминантне компоненте њихових одбрамбених секрета хинони. Поред
хинона, у секретима озадена Julida регистровани су и алкохоли, алдехиди, кетони,
фенолна једињења, као и бројни естри засићених и незасићених карбоксилних
киселина. Досадашња истраживања су показала да су екстракти одбрамбених
секрета Julida, као и појединачна једињења која у њихов састав улазе, биолошки
активни природни производи, али њихов цитотоксични потенцијал није довољно
истражен. У овој студији је испитиван утицај различитих естара линолеинске
киселине (бутил-, пентил-, хексил-, хептил-, октил-, нонил-, фенетил- и 3-
фенилпропил-линолеат) детектованих у одбрамбеним секретима стонога
Megaphyllum bosniense и M. unilineatum на вијабилност нормалних (BEAS-2B) и
канцeрских (A549) ћелија плућа коришћењем МТТ теста. Сви испитивани естри су
смањивали вијабилност ћелија, при чему је постојала значајна разлика у одговору
канцерских у односу на нормалне ћелије у случају три естра (хексил-, фенетил- и 3-
фенилпропил-линолеат). Иако су естри карбоксилних киселина познати као мало
реактивна једињења, резултати ове студије показују да представници ове класе
хемијских једињења могу имати цитотоксични потенцијал.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida), Цитотоксични потенцијал естара линолеинске киселине детектованих у одбрамбеним секретима стонога Megaphyllum bosniense и M. unilineatum (Diplopoda: Julida)",
pages = "186",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1731"
}

Milovanović, J., Ilić, B., Radulović, N., Mladenović, M., Makarov, S.,& Divac Rankov, A.. (2022). Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida). in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 186.
https://hdl.handle.net/21.15107/rcub_imagine_1731

Milovanović J, Ilić B, Radulović N, Mladenović M, Makarov S, Divac Rankov A. Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida). in Treći kongres biologa Srbije. 2022;:186.
https://hdl.handle.net/21.15107/rcub_imagine_1731 .

Milovanović, Jelena, Ilić, Bojan, Radulović, Niko, Mladenović, Marko, Makarov, Slobodan, Divac Rankov, Aleksandra, "Citotoksični potencijal estara linoleinske kiseline detektovanih u odbrambenim sekretima stonoga Megaphyllum bosniense i M. unilineatum (Diplopoda: Julida)" in Treći kongres biologa Srbije (2022):186,
https://hdl.handle.net/21.15107/rcub_imagine_1731 .

TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Bozić, Bojan
AU  - Lunić, Tanja
AU  - Bacić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Nedeljković, Biljana Bozic
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1567
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - MDPI, Basel
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis
IS  - 6
VL  - 14
DO  - 10.3390/nu14061273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Bozić, Bojan and Lunić, Tanja and Bacić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Nedeljković, Biljana Bozic",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "MDPI, Basel",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis",
number = "6",
volume = "14",
doi = "10.3390/nu14061273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Bozić, B., Lunić, T., Bacić, A., Rajilić-Stojanović, M., Peković, S.,& Nedeljković, B. B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients
MDPI, Basel., 14(6).
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Bozić B, Lunić T, Bacić A, Rajilić-Stojanović M, Peković S, Nedeljković BB. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis. in Nutrients. 2022;14(6).
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Bozić, Bojan, Lunić, Tanja, Bacić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Nedeljković, Biljana Bozic, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis" in Nutrients, 14, no. 6 (2022),
https://doi.org/10.3390/nu14061273 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Gardijan, Lazar
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1572
AB  - The rapid and appropriate response of Pseudomonas spp. to environmental fluctuations has been enabled by numerous signal transduction regulatory systems. Regulatory systems in Pseudomonas aeruginosa are organized in a complex network which provides quick and fine-tuned cellular response through regulation of virulence and antibiotic resistance determinants production. Mobile integrons represent genetic elements included in the rapid dissemination of multiple antibiotic resistance determinants. The key factor of integron dynamics is enzyme integrase. So far, global regulators LexA, RpoS and PsrA have been recognized as regulators of the intI1 transcription. In this study, we discovered novel activator of the intI1 transcription, sensor kinase RclS, in Pseudomonas putida WCS358. This regulation is limited to stationary growth phase and appears to be indirect, at least through regulation of the rpoS expression. Sensor kinase RclS is a part of novel three-component system Rcl (Roc-like) together with two response regulators, RclR and RclA. RclS acted as a negative regulator of the rclA transcription, while the role in the rclR transcription regulation could not be defined. The RclSAR regulatory system seems to be a part of complex intI1 regulatory network which includes major stress response (SOS and RpoS) regulons.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase
IS  - 1-2
VL  - 173
DO  - 10.1016/j.resmic.2021.103885
ER  - 

@article{
author = "Novović, Katarina and Malešević, Milka and Gardijan, Lazar and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "The rapid and appropriate response of Pseudomonas spp. to environmental fluctuations has been enabled by numerous signal transduction regulatory systems. Regulatory systems in Pseudomonas aeruginosa are organized in a complex network which provides quick and fine-tuned cellular response through regulation of virulence and antibiotic resistance determinants production. Mobile integrons represent genetic elements included in the rapid dissemination of multiple antibiotic resistance determinants. The key factor of integron dynamics is enzyme integrase. So far, global regulators LexA, RpoS and PsrA have been recognized as regulators of the intI1 transcription. In this study, we discovered novel activator of the intI1 transcription, sensor kinase RclS, in Pseudomonas putida WCS358. This regulation is limited to stationary growth phase and appears to be indirect, at least through regulation of the rpoS expression. Sensor kinase RclS is a part of novel three-component system Rcl (Roc-like) together with two response regulators, RclR and RclA. RclS acted as a negative regulator of the rclA transcription, while the role in the rclR transcription regulation could not be defined. The RclSAR regulatory system seems to be a part of complex intI1 regulatory network which includes major stress response (SOS and RpoS) regulons.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase",
number = "1-2",
volume = "173",
doi = "10.1016/j.resmic.2021.103885"
}

Novović, K., Malešević, M., Gardijan, L., Kojić, M.,& Jovčić, B.. (2022). Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase. in Research in Microbiology
Elsevier, Amsterdam., 173(1-2).
https://doi.org/10.1016/j.resmic.2021.103885

Novović K, Malešević M, Gardijan L, Kojić M, Jovčić B. Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase. in Research in Microbiology. 2022;173(1-2).
doi:10.1016/j.resmic.2021.103885 .

Novović, Katarina, Malešević, Milka, Gardijan, Lazar, Kojić, Milan, Jovčić, Branko, "Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase" in Research in Microbiology, 173, no. 1-2 (2022),
https://doi.org/10.1016/j.resmic.2021.103885 . .