TY  - THES
AU  - Bosnić, Dragana
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7762
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22993/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=24873737
UR  - https://nardus.mpn.gov.rs/handle/123456789/17638
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/60
AB  - Bakar (Cu) je esencijalan mikrolement za biljke, dok je prisustvo Cu u višku fitotoksično i izaziva oksidativni stres. Silicijum (Si) ne pripada grupi esencijalnih već korisnih elemenata, a jedini je element koji ispoljava protektivan efekat na biljke izložene stresnim uslovima. Uloga Si u stresu izazvanom toksičnim koncentracijama Cu nije dovoljno istražena. Cilj ovog rada bio je da se ispitaju mehanizmi delovanja Si u takvim uslovima kod krastavca, akumulatora Si. Rezultati su pokazali da primena Si povećava toleranciju biljaka na toksičnost Cu. Mehanizmi delovanja Si su usmereni ka snižavanju ekspresije gena odgovornih za usvajanje Cu i, posledično, smanjenju koncentracije Cu u biljkama, kao i ka ublažavanju disbalansa drugih esencijalnih mikroelemenata, pre svega gvožđa. Na taj način, Si je smanjio hlorozu i povećao biomasu listova biljaka. Si je doprineo preraspodeli Cu na nivou ćelija korena, povećavajući imobilizaciju Cu u ćelijskim zidovima. Povećanom akumulacijom liganada koji vezuju Cu, kao što su organske kiseline (citrat i malat u korenu, a akonitat u listu) i aminokiseline (nikocijanamin i histidin u listu), Si je ublažio toksične efekte Cu unutar ćelije. Pored toga, stimulacijom aktivnosti antioksidativnih enzima, smanjen je nivo oksidativnog stresa. Primena Si je uticala na glavne regulatore homeostaze Cu, mikro RNK (miR398 i miR408), čija je snižena ekspresija u korenu omogućila povišenu ekspresiju njihovih targeta: Cu/Zn superoksid-dismutaze 2 (CSD2) i lakaze 3. U skladu sa tim, akumulacija Cu-proteina, CSD2 u korenu i plastocijanina u listu, kao primarnih depoa Cu jona je izraženija kod biljaka gajenih sa Si. Razumevanje mehanizama delovanja Si u biljkama će doprineti njegovoj široj primeni u cilju povećanja tolerancije biljaka na stres.
AB  - Copper (Cu) is an essential microelement for plants, although it is phytotoxic in excess, causing oxidative stress. Silicon (Si) does not belong to the essential but beneficial elements, and it is the only known element with protective effect to plants exposed to stress. The role of Si in plants exposed to Cu-stress has not been thoroughly studied so far. The aim of this study was to investigate the Si-mediated mechanisms under such conditions in cucumber plants, a Si-accumulating species. The results showed that Si application enhanced plant tolerance to Cu in excess. Si-mediated mechanisms were directed towards downregulation of the genes involved in Cu uptake, thus decreasing Cu concentration in the treated plants, as well as alleviating imbalance of other microelements, particularly Fe. Consequently, Si alleviated chlorosis supporting higher leaf biomass production in these plants. Si contributed to the redistribution of Cu at the root cell level, by increasing the immobilization of Cu in the cell walls. Higher accumulation of Cu-binding ligands such as organic acids (citrate and malate in the roots and aconitate in the leaves) and amino acids (nicotianamine and histidine in the leaves) diminished Cu-toxicity effects inside the cells. Additionally, a decreased level of oxidative stress was achieved by Si-stimulated activities of antioxidative enzymes. Si-application downregulated the expression of micro RNAs (miR398 and miR408), the main regulators of Cu homeostasis, thus enabling higher expression of their target genes: Cu/Zn superoxide dismutase 2 (CSD2) and laccase 3. Accordingly, accumulation of Cu-proteins, CSD2 in the roots and plastocyanine in the leaves, as “buffering sinks” for Cu, was obtained in the plants grown with Si. Understanding the Si-mediated mechanisms in plants will contribute to its broader application with the aim to increase plant tolerance to stress.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Uloga silicijumove kiseline u modulaciji odgovora krastavca (Cucumis sativus L.) na oksidativni stres izazvan toksičnim koncentracijama bakra
T1  - The role of silicic acid in modulation of oxidative stress responses in cucumber (Cucumis sativus L.) exposed to toxic copper
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17638
ER  - 

@phdthesis{
author = "Bosnić, Dragana",
year = "2020",
abstract = "Bakar (Cu) je esencijalan mikrolement za biljke, dok je prisustvo Cu u višku fitotoksično i izaziva oksidativni stres. Silicijum (Si) ne pripada grupi esencijalnih već korisnih elemenata, a jedini je element koji ispoljava protektivan efekat na biljke izložene stresnim uslovima. Uloga Si u stresu izazvanom toksičnim koncentracijama Cu nije dovoljno istražena. Cilj ovog rada bio je da se ispitaju mehanizmi delovanja Si u takvim uslovima kod krastavca, akumulatora Si. Rezultati su pokazali da primena Si povećava toleranciju biljaka na toksičnost Cu. Mehanizmi delovanja Si su usmereni ka snižavanju ekspresije gena odgovornih za usvajanje Cu i, posledično, smanjenju koncentracije Cu u biljkama, kao i ka ublažavanju disbalansa drugih esencijalnih mikroelemenata, pre svega gvožđa. Na taj način, Si je smanjio hlorozu i povećao biomasu listova biljaka. Si je doprineo preraspodeli Cu na nivou ćelija korena, povećavajući imobilizaciju Cu u ćelijskim zidovima. Povećanom akumulacijom liganada koji vezuju Cu, kao što su organske kiseline (citrat i malat u korenu, a akonitat u listu) i aminokiseline (nikocijanamin i histidin u listu), Si je ublažio toksične efekte Cu unutar ćelije. Pored toga, stimulacijom aktivnosti antioksidativnih enzima, smanjen je nivo oksidativnog stresa. Primena Si je uticala na glavne regulatore homeostaze Cu, mikro RNK (miR398 i miR408), čija je snižena ekspresija u korenu omogućila povišenu ekspresiju njihovih targeta: Cu/Zn superoksid-dismutaze 2 (CSD2) i lakaze 3. U skladu sa tim, akumulacija Cu-proteina, CSD2 u korenu i plastocijanina u listu, kao primarnih depoa Cu jona je izraženija kod biljaka gajenih sa Si. Razumevanje mehanizama delovanja Si u biljkama će doprineti njegovoj široj primeni u cilju povećanja tolerancije biljaka na stres., Copper (Cu) is an essential microelement for plants, although it is phytotoxic in excess, causing oxidative stress. Silicon (Si) does not belong to the essential but beneficial elements, and it is the only known element with protective effect to plants exposed to stress. The role of Si in plants exposed to Cu-stress has not been thoroughly studied so far. The aim of this study was to investigate the Si-mediated mechanisms under such conditions in cucumber plants, a Si-accumulating species. The results showed that Si application enhanced plant tolerance to Cu in excess. Si-mediated mechanisms were directed towards downregulation of the genes involved in Cu uptake, thus decreasing Cu concentration in the treated plants, as well as alleviating imbalance of other microelements, particularly Fe. Consequently, Si alleviated chlorosis supporting higher leaf biomass production in these plants. Si contributed to the redistribution of Cu at the root cell level, by increasing the immobilization of Cu in the cell walls. Higher accumulation of Cu-binding ligands such as organic acids (citrate and malate in the roots and aconitate in the leaves) and amino acids (nicotianamine and histidine in the leaves) diminished Cu-toxicity effects inside the cells. Additionally, a decreased level of oxidative stress was achieved by Si-stimulated activities of antioxidative enzymes. Si-application downregulated the expression of micro RNAs (miR398 and miR408), the main regulators of Cu homeostasis, thus enabling higher expression of their target genes: Cu/Zn superoxide dismutase 2 (CSD2) and laccase 3. Accordingly, accumulation of Cu-proteins, CSD2 in the roots and plastocyanine in the leaves, as “buffering sinks” for Cu, was obtained in the plants grown with Si. Understanding the Si-mediated mechanisms in plants will contribute to its broader application with the aim to increase plant tolerance to stress.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Uloga silicijumove kiseline u modulaciji odgovora krastavca (Cucumis sativus L.) na oksidativni stres izazvan toksičnim koncentracijama bakra, The role of silicic acid in modulation of oxidative stress responses in cucumber (Cucumis sativus L.) exposed to toxic copper",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17638"
}

Bosnić, D.. (2020). Uloga silicijumove kiseline u modulaciji odgovora krastavca (Cucumis sativus L.) na oksidativni stres izazvan toksičnim koncentracijama bakra. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_17638

Bosnić D. Uloga silicijumove kiseline u modulaciji odgovora krastavca (Cucumis sativus L.) na oksidativni stres izazvan toksičnim koncentracijama bakra. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_17638 .

Bosnić, Dragana, "Uloga silicijumove kiseline u modulaciji odgovora krastavca (Cucumis sativus L.) na oksidativni stres izazvan toksičnim koncentracijama bakra" (2020),
https://hdl.handle.net/21.15107/rcub_nardus_17638 .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Kojić, Milorad
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1392
AB  - The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adri, did4, kell and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Fungal Genetics and Biology
T1  - A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations
VL  - 134
DO  - 10.1016/j.fgb.2019.103284
ER  - 

@article{
author = "Milisavljević, Mira and Kojić, Milorad",
year = "2020",
abstract = "The ability of Saccharomyces cerevisiae to reconstitute viability after strong peroxide-induced oxidative stress during liquid holding (LH) in non-nutrient medium has been compared with that of Ustilago maydis. It was found that like U. maydis, S. cerevisiae was capable of reconstituting viability through multiplication of the survivors. However, differences were observed in the pattern of their response: (i) the reconstitution of viability was slower in S. cerevisiae; (ii) before the viability was progressively increasing the treated samples of this fungus reproducibly passed through a phase of additional decrease of the surviving fraction and (iii) the final yields of viable cells attained in S. cerevisiae were below those achieved by U. maydis. The reason for the relative superiority of U. maydis is twofold: (1) early initiated and faster degradation and leakage of the intracellular biomolecules and (2) greater ability of U. maydis cells to recycle damaged and released intracellular compounds. Conceptually similar studies extended to another oxidative-stress-inducing condition, namely desiccation, indicated that the marked differences between these fungi in their patterns of the post-stress regrowth, cellular leakage and macromolecule decomposition are reproduced during LH of desiccated cells. The concordance of the findings obtained upon these two approaches was also corroborated by an analysis of the post-desiccation LH response of U. maydis mutants (adri, did4, kell and tbp1) that were previously identified as defective in post-peroxide LH restitution of viability. We discuss the findings in terms of their possible relevance to the mechanisms of the ecological and evolutionary adaptation of free-living microorganisms to fluctuating and severely inhospitable environments.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Fungal Genetics and Biology",
title = "A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations",
volume = "134",
doi = "10.1016/j.fgb.2019.103284"
}

Milisavljević, M.,& Kojić, M.. (2020). A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. in Fungal Genetics and Biology
Academic Press Inc Elsevier Science, San Diego., 134.
https://doi.org/10.1016/j.fgb.2019.103284

Milisavljević M, Kojić M. A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations. in Fungal Genetics and Biology. 2020;134.
doi:10.1016/j.fgb.2019.103284 .

Milisavljević, Mira, Kojić, Milorad, "A comparative study of liquid holding restitution of viability after oxidative stress in Ustilago maydis and Saccharomyces cerevisiae cell populations" in Fungal Genetics and Biology, 134 (2020),
https://doi.org/10.1016/j.fgb.2019.103284 . .

TY  - JOUR
AU  - Bosnić, Dragana
AU  - Bosnić, Predrag
AU  - Nikolić, Dragana
AU  - Nikolić, Miroslav
AU  - Samardžić, Jelena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1214
AB  - Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves
IS  - 12
VL  - 8
DO  - 10.3390/plants8120554
ER  - 

@article{
author = "Bosnić, Dragana and Bosnić, Predrag and Nikolić, Dragana and Nikolić, Miroslav and Samardžić, Jelena",
year = "2019",
abstract = "Copper (Cu) toxicity in plants may lead to iron (Fe), zinc (Zn) and manganese (Mn) deficiencies. Here, we investigated the effect of Si and Fe supply on the concentrations of micronutrients and metal-chelating amino acids nicotianamine (NA) and histidine (His) in leaves of cucumber plants exposed to Cu in excess. Cucumber (Cucumis sativus L.) was treated with 10 mu M Cu, and additional 100 mu M Fe or/and 1.5 mM Si for five days. High Cu and decreased Zn, Fe and Mn concentrations were found in Cu treatment. Additional Fe supply had a more pronounced effect in decreasing Cu accumulation and improving the molar ratio between micronutrients as compared to the Si supply. However, the simultaneous supply of Fe and Si was the most effective treatment in alleviation of Cu-induced deficiency of Fe, Zn and Mn. Additional Fe supply increased the His but not NA concentration, while Si supply significantly increased both NA and His whereby the NA:Cu and His:Cu molar ratios exceeded the control values indicating that Si recruits Cu-chelation to achieve Cu tolerance. In conclusion, Si-mediated alleviation of Cu toxicity was directed toward Cu tolerance while Fe-alleviative effect was due to a dramatic decrease in Cu accumulation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves",
number = "12",
volume = "8",
doi = "10.3390/plants8120554"
}

Bosnić, D., Bosnić, P., Nikolić, D., Nikolić, M.,& Samardžić, J.. (2019). Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel
MDPI, Basel., 8(12).
https://doi.org/10.3390/plants8120554

Bosnić D, Bosnić P, Nikolić D, Nikolić M, Samardžić J. Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves. in Plants-Basel. 2019;8(12).
doi:10.3390/plants8120554 .

Bosnić, Dragana, Bosnić, Predrag, Nikolić, Dragana, Nikolić, Miroslav, Samardžić, Jelena, "Silicon and Iron Differently Alleviate Copper Toxicity in Cucumber Leaves" in Plants-Basel, 8, no. 12 (2019),
https://doi.org/10.3390/plants8120554 . .

TY  - JOUR
AU  - Nikolić, Dragana
AU  - Nešić, Sofija
AU  - Bosnić, Dragana
AU  - Kostić, Ljiljana
AU  - Nikolić, Miroslav
AU  - Samardžić, Jelena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1289
AB  - The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Plant Science
T1  - Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution
VL  - 10
DO  - 10.3389/fpls.2019.00416
ER  - 

@article{
author = "Nikolić, Dragana and Nešić, Sofija and Bosnić, Dragana and Kostić, Ljiljana and Nikolić, Miroslav and Samardžić, Jelena",
year = "2019",
abstract = "The beneficial effects of silicon (Si) have been shown on plants using reduction-based strategy for iron (Fe) acquisition. Here we investigated the influence of Si on Fe deficiency stress alleviation in barley (Hordeum vulgare), a crop plant which uses the chelation-based strategy for Fe acquisition. Analyses of chlorophyll content, ROS accumulation, antioxidative status, concentrations of Fe and other micronutrients, along with the expression of Strategy II genes were studied in response to Si supply. Si successfully ameliorated Fe deficiency in barley, diminishing chlorophyll and biomass loss, and improving the activity of antioxidative enzymes, resulting in lowered reactive oxidative species accumulation in the youngest leaves. Alleviation of Fe deficiency stress correlated well with the Si-induced increase of Fe content in the youngest leaves, while it was decreased in root. Moreover, Si nutrition lowered accumulation of other micronutrients in the youngest leaves of Fe deprived plants, by retaining them in the root. On the transcriptional level, Si led to an expedient increase in the expression of genes involved in Strategy II Fe acquisition in roots at the early stage of Fe deficiency stress, while decreasing their expression in a prolonged stress response. Expression of Strategy II genes was remarkably upregulated in the leaves of Si supplied plants. This study broadens the perspective of mechanisms of Si action, providing evidence for ameliorative effects of Si on Strategy II plants, including its influence on accumulation and distribution of microelements, as well as on the expression of the Strategy II genes.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Plant Science",
title = "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution",
volume = "10",
doi = "10.3389/fpls.2019.00416"
}

Nikolić, D., Nešić, S., Bosnić, D., Kostić, L., Nikolić, M.,& Samardžić, J.. (2019). Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science
Frontiers Media Sa, Lausanne., 10.
https://doi.org/10.3389/fpls.2019.00416

Nikolić D, Nešić S, Bosnić D, Kostić L, Nikolić M, Samardžić J. Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution. in Frontiers in Plant Science. 2019;10.
doi:10.3389/fpls.2019.00416 .

Nikolić, Dragana, Nešić, Sofija, Bosnić, Dragana, Kostić, Ljiljana, Nikolić, Miroslav, Samardžić, Jelena, "Silicon Alleviates Iron Deficiency in Barley by Enhancing Expression of Strategy II Genes and Metal Redistribution" in Frontiers in Plant Science, 10 (2019),
https://doi.org/10.3389/fpls.2019.00416 . .

TY  - JOUR
AU  - Cujić-Nikolić, Nada
AU  - Stanisavljević, Nemanja
AU  - Savikin, Katarina
AU  - Kaluević, Ana
AU  - Nedović, Viktor
AU  - Samardžić, Jelena
AU  - Janković, Teodora
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1215
AB  - Aim: Microencapsulation of chokeberry extracts was performed in order to improve functionality, stability, and bioavailability of extracted polyphenols. Methods: Chokeberry fruits and juice by-product (waste) extracts were spray-dried by using two carriers, maltodextrin and skimmed milk. Morphological and physicochemical characteristics of the obtained powders were analysed. In vitro simulated digestion model was used as an indicator of polyphenolics bioavailability. Results: The moisture content varied between 3.39 and 4.61%, zeta potential had negative values (35-39 mV), maltodetrin powders were smaller (4.27-5.12 mu m) compared to skimmed ones (8.50-11.01 mu m). All microparticles exhibited high encapsulation efficiency of total polyphenols and anthocyanins (73-97% and 63-96%, respectively). For both extract types, maltodextrin powders released higher phenolics content compared to skimmed milk. During in vitro digestion, maltodextrin exhibited a higher protective effect on both active compounds. Conclusion: Taking into account the obtained results, chokeberry polyphenols stability might be improved using spray drying technique, and maltodextrin showed better properties.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion
EP  - 703
IS  - 8
SP  - 693
VL  - 36
DO  - 10.1080/02652048.2019.1667448
ER  - 

@article{
author = "Cujić-Nikolić, Nada and Stanisavljević, Nemanja and Savikin, Katarina and Kaluević, Ana and Nedović, Viktor and Samardžić, Jelena and Janković, Teodora",
year = "2019",
abstract = "Aim: Microencapsulation of chokeberry extracts was performed in order to improve functionality, stability, and bioavailability of extracted polyphenols. Methods: Chokeberry fruits and juice by-product (waste) extracts were spray-dried by using two carriers, maltodextrin and skimmed milk. Morphological and physicochemical characteristics of the obtained powders were analysed. In vitro simulated digestion model was used as an indicator of polyphenolics bioavailability. Results: The moisture content varied between 3.39 and 4.61%, zeta potential had negative values (35-39 mV), maltodetrin powders were smaller (4.27-5.12 mu m) compared to skimmed ones (8.50-11.01 mu m). All microparticles exhibited high encapsulation efficiency of total polyphenols and anthocyanins (73-97% and 63-96%, respectively). For both extract types, maltodextrin powders released higher phenolics content compared to skimmed milk. During in vitro digestion, maltodextrin exhibited a higher protective effect on both active compounds. Conclusion: Taking into account the obtained results, chokeberry polyphenols stability might be improved using spray drying technique, and maltodextrin showed better properties.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion",
pages = "703-693",
number = "8",
volume = "36",
doi = "10.1080/02652048.2019.1667448"
}

Cujić-Nikolić, N., Stanisavljević, N., Savikin, K., Kaluević, A., Nedović, V., Samardžić, J.,& Janković, T.. (2019). Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion. in Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 36(8), 693-703.
https://doi.org/10.1080/02652048.2019.1667448

Cujić-Nikolić N, Stanisavljević N, Savikin K, Kaluević A, Nedović V, Samardžić J, Janković T. Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion. in Journal of Microencapsulation. 2019;36(8):693-703.
doi:10.1080/02652048.2019.1667448 .

Cujić-Nikolić, Nada, Stanisavljević, Nemanja, Savikin, Katarina, Kaluević, Ana, Nedović, Viktor, Samardžić, Jelena, Janković, Teodora, "Chokeberry polyphenols preservation using spray drying: effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion" in Journal of Microencapsulation, 36, no. 8 (2019):693-703,
https://doi.org/10.1080/02652048.2019.1667448 . .

TY  - JOUR
AU  - Bosnić, Dragana
AU  - Nikolić, Dragana
AU  - Timotijević, Gordana
AU  - Pavlović, Jelena
AU  - Vaculik, Marek
AU  - Samardžić, Jelena
AU  - Nikolić, Miroslav
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1204
AB  - Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity
EP  - 641
IS  - 1-2
SP  - 629
VL  - 441
DO  - 10.1007/s11104-019-04151-5
ER  - 

@article{
author = "Bosnić, Dragana and Nikolić, Dragana and Timotijević, Gordana and Pavlović, Jelena and Vaculik, Marek and Samardžić, Jelena and Nikolić, Miroslav",
year = "2019",
abstract = "Aims Although silicon (Si) is known to increase plant resistance to metal toxicity stress, the mechanisms responsible for alleviation of copper (Cu) toxicity are still insufficiently clear. We investigated the role of Si on Cu-binding processes involved in buffering excessive Cu in cucumber (Cucumis sativus L.) tissues. Methods Cucumber plants were subjected to moderate Cu toxicity stress (10 mu M Cu) without (-Si) or with (+Si) supply of 1.5 mM Si. We analyzed total and cell wall concentrations of Cu and Cu-binding compounds (organic acids and Cu-proteins) along with parameters of oxidative stress (e.g. lipid peroxidation and lignification). Results Supply of Si decreased total Cu concentration in both root and leaf tissues, but increased the root cell wall Cu fraction. Also, Si increased superoxide dismutase (SOD) activity in 10 mu M Cu-treated plants. Concomitantly, protein levels of Cu/Zn SOD isoforms (CSD1 and CSD2) in root tissues also increased in +Si plants. The leaf Cu-binding compounds, such as aconitate and plastocyanin (including the expression of CsPC gene) were higher in the +Si plants. Consequently, Si supply effectively lowered lipid peroxidation in both roots and leaves of Cu-stressed plants. Conclusions Supply of Si enhanced both the accumulation of Cu-binding molecules (Zn/Cu SOD in roots; aconitate and plastocyanin in leaves), and the Cu-binding capacity of the root cell wall.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity",
pages = "641-629",
number = "1-2",
volume = "441",
doi = "10.1007/s11104-019-04151-5"
}

Bosnić, D., Nikolić, D., Timotijević, G., Pavlović, J., Vaculik, M., Samardžić, J.,& Nikolić, M.. (2019). Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil
Springer, Dordrecht., 441(1-2), 629-641.
https://doi.org/10.1007/s11104-019-04151-5

Bosnić D, Nikolić D, Timotijević G, Pavlović J, Vaculik M, Samardžić J, Nikolić M. Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity. in Plant and Soil. 2019;441(1-2):629-641.
doi:10.1007/s11104-019-04151-5 .

Bosnić, Dragana, Nikolić, Dragana, Timotijević, Gordana, Pavlović, Jelena, Vaculik, Marek, Samardžić, Jelena, Nikolić, Miroslav, "Silicon alleviates copper (Cu) toxicity in cucumber by increased Cu-binding capacity" in Plant and Soil, 441, no. 1-2 (2019):629-641,
https://doi.org/10.1007/s11104-019-04151-5 . .

TY  - JOUR
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
AU  - Holloman, William K.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1190
AB  - Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.
PB  - Elsevier Science Bv, Amsterdam
T2  - DNA Repair
T1  - Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis
EP  - 55
SP  - 47
VL  - 63
DO  - 10.1016/j.dnarep.2018.01.010
ER  - 

@article{
author = "Kojić, Milorad and Milisavljević, Mira and Holloman, William K.",
year = "2018",
abstract = "Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "DNA Repair",
title = "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis",
pages = "55-47",
volume = "63",
doi = "10.1016/j.dnarep.2018.01.010"
}

Kojić, M., Milisavljević, M.,& Holloman, W. K.. (2018). Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair
Elsevier Science Bv, Amsterdam., 63, 47-55.
https://doi.org/10.1016/j.dnarep.2018.01.010

Kojić M, Milisavljević M, Holloman WK. Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair. 2018;63:47-55.
doi:10.1016/j.dnarep.2018.01.010 .

Kojić, Milorad, Milisavljević, Mira, Holloman, William K., "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis" in DNA Repair, 63 (2018):47-55,
https://doi.org/10.1016/j.dnarep.2018.01.010 . .

TY  - JOUR
AU  - Ćujić-Nikolić, Nada
AU  - Stanisavljević, Nemanja
AU  - Šavikin, Katarina
AU  - Kalušević, Ana
AU  - Nedović, Viktor
AU  - Bigović, Dubravka
AU  - Janković, Teodora
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1155
AB  - Due to its high content of phenolics, black chokeberry has been proposed as a health-promoting material, especially use of chokeberry extract as a food or pharmaceuticals. Chokeberry extract was spray-dried using gum Arabic as a carrier. The optimal conditions for the efficient microencapsulation of chokeberry extract using spray-drying technique have been investigated. The aim of our study was to obtain microparticles with the best potential to improve functionality and stability of extracted chokeberry polyphenols, and to investigate the possibility of microbeads to protect active compounds during simulated digestion process. Morphological characteristics of microbeads were analyzed using FTIR and SEM techniques. Zeta potential, particle size and moisture content were determined. Released total phenolics and total anthocyanins content as well as concentration of individual anthocyanins were quantified before and after digestion process. Microparticles exhibited high encapsulation efficiency up to 87%, and high content of released polyphenols was achieved. After in vitro simulated digestion phenolic compounds decreased by 11-24%, exhibited higher protective effect of gum Arabic. Our results showed that chokeberry microparticles obtained by spray drying method could be useful supplements or functional food. .
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method
EP  - 16
IS  - 38
SP  - 9
DO  - 10.5937/leksir1838009C
ER  - 

@article{
author = "Ćujić-Nikolić, Nada and Stanisavljević, Nemanja and Šavikin, Katarina and Kalušević, Ana and Nedović, Viktor and Bigović, Dubravka and Janković, Teodora",
year = "2018",
abstract = "Due to its high content of phenolics, black chokeberry has been proposed as a health-promoting material, especially use of chokeberry extract as a food or pharmaceuticals. Chokeberry extract was spray-dried using gum Arabic as a carrier. The optimal conditions for the efficient microencapsulation of chokeberry extract using spray-drying technique have been investigated. The aim of our study was to obtain microparticles with the best potential to improve functionality and stability of extracted chokeberry polyphenols, and to investigate the possibility of microbeads to protect active compounds during simulated digestion process. Morphological characteristics of microbeads were analyzed using FTIR and SEM techniques. Zeta potential, particle size and moisture content were determined. Released total phenolics and total anthocyanins content as well as concentration of individual anthocyanins were quantified before and after digestion process. Microparticles exhibited high encapsulation efficiency up to 87%, and high content of released polyphenols was achieved. After in vitro simulated digestion phenolic compounds decreased by 11-24%, exhibited higher protective effect of gum Arabic. Our results showed that chokeberry microparticles obtained by spray drying method could be useful supplements or functional food. .",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method",
pages = "16-9",
number = "38",
doi = "10.5937/leksir1838009C"
}

Ćujić-Nikolić, N., Stanisavljević, N., Šavikin, K., Kalušević, A., Nedović, V., Bigović, D.,& Janković, T.. (2018). Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(38), 9-16.
https://doi.org/10.5937/leksir1838009C

Ćujić-Nikolić N, Stanisavljević N, Šavikin K, Kalušević A, Nedović V, Bigović D, Janković T. Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method. in Lekovite sirovine. 2018;(38):9-16.
doi:10.5937/leksir1838009C .

Ćujić-Nikolić, Nada, Stanisavljević, Nemanja, Šavikin, Katarina, Kalušević, Ana, Nedović, Viktor, Bigović, Dubravka, Janković, Teodora, "Application of gum Arabic in the production of spray-dried chokeberry polyphenols, microparticles characterisation and in vitro digestion method" in Lekovite sirovine, no. 38 (2018):9-16,
https://doi.org/10.5937/leksir1838009C . .

TY  - JOUR
AU  - Kojić, Milorad
AU  - Milisavljević, Mira
AU  - Holloman, William K.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1626
AB  - Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.
PB  - Elsevier Science Bv, Amsterdam
T2  - DNA Repair
T1  - Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis
EP  - 55
SP  - 47
VL  - 63
DO  - 10.1016/j.dnarep.2018.01.010
ER  - 

@article{
author = "Kojić, Milorad and Milisavljević, Mira and Holloman, William K.",
year = "2018",
abstract = "Cells maintain a small arsenal of resolving functions to process and eliminate complex DNA intermediates that result as a consequence of homologous recombination and distressed replication. Ordinarily the homologous recombination system serves as a high-fidelity mechanism to restore the integrity of a damaged genome, but in the absence of the appropriate resolving function it can turn DNA intermediates resulting from replication stress into pathological forms that are toxic to cells. Here we have investigated how the nucleases Mus81 and Gen1 and the helicase Blm contribute to survival after DNA damage or replication stress in Ustilago maydis cells with crippled yet homologous recombination-proficient forms of Brh2, the BRCA2 ortholog and primary Rad51 mediator. We found collaboration among the factors. Notable were three findings. First, the ability of Gen1 to rescue hydroxyurea sensitivity of dysfunctional Blm requires the absence of Mus81. Second, the response of mutants defective in Blm and Gen1 to hydroxyurea challenge is markedly similar suggesting cooperation of these factors in the same pathway. Third, the repair proficiency of Brh2 mutant variants deleted of its N-terminal DNA binding region requires not only Rad52 but also Gen1. and Mus81. We suggest these factors comprise a sub pathway for channeling repair when Brh2 is compromised in its interplay with DNA.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "DNA Repair",
title = "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis",
pages = "55-47",
volume = "63",
doi = "10.1016/j.dnarep.2018.01.010"
}

Kojić, M., Milisavljević, M.,& Holloman, W. K.. (2018). Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair
Elsevier Science Bv, Amsterdam., 63, 47-55.
https://doi.org/10.1016/j.dnarep.2018.01.010

Kojić M, Milisavljević M, Holloman WK. Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis. in DNA Repair. 2018;63:47-55.
doi:10.1016/j.dnarep.2018.01.010 .

Kojić, Milorad, Milisavljević, Mira, Holloman, William K., "Collaboration in the actions of Brh2 with resolving functions during DNA repair and replication stress in Ustilago maydis" in DNA Repair, 63 (2018):47-55,
https://doi.org/10.1016/j.dnarep.2018.01.010 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Petković, Jelena
AU  - Samardžić, Jelena
AU  - Kojić, Milorad
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1186
AB  - After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations
VL  - 9
DO  - 10.3389/fmicb.2018.00990
ER  - 

@article{
author = "Milisavljević, Mira and Petković, Jelena and Samardžić, Jelena and Kojić, Milorad",
year = "2018",
abstract = "After heavy exposure of Ustilago maydis cells to clastogens, a great increase in viability was observed if the treated cells were kept under starvation conditions This restitution of viability is based on cell multiplication at the expense of the intracellular compounds freed from the damaged cells. Analysis of the effect of the leaked material on the growth of undamaged cells revealed opposing biological activity, indicating that U. maydis must possess cellular mechanisms involved not only in reabsorption of the released compounds from external environment but also in contending with their treatment-induced toxicity. From a screen for mutants defective in the restitution of viability, we identified four genes (adr1, did4, kel1, and tbp1) that contribute to the process The mutants in did4, kel1, and tbp1 exhibited sensitivity to different genotoxic agents implying that the gene products are in some overlapping fashion involved in the protection of genome integrity The genetic determinants identified by our analysis have already been known to play roles in growth regulation, protein turnover, cytoskeleton structure, and transcription. We discuss ecological and evolutionary implications of these results.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations",
volume = "9",
doi = "10.3389/fmicb.2018.00990"
}

Milisavljević, M., Petković, J., Samardžić, J.,& Kojić, M.. (2018). Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fmicb.2018.00990

Milisavljević M, Petković J, Samardžić J, Kojić M. Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations. in Frontiers in Microbiology. 2018;9.
doi:10.3389/fmicb.2018.00990 .

Milisavljević, Mira, Petković, Jelena, Samardžić, Jelena, Kojić, Milorad, "Bioavailability of Nutritional Resources From Cells Killed by Oxidation Supports Expansion of Survivors in Ustilago maydis Populations" in Frontiers in Microbiology, 9 (2018),
https://doi.org/10.3389/fmicb.2018.00990 . .

TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Pajić, Vesna
AU  - Dudić, Dragana
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1191
AB  - Primary goals of 21st century science involve eco-friendly solutions for detection, control and suppression of plant viruses. Even though we are accumulating knowledge and data on plant viruses' nucleotide sequences, we are still using a minimum of information available from the collected data. Applying bioinformatics tools and data mining approach to viral sequences is extremely useful in revealing the hidden knowledge, giving guidelines for further biological/bioinformatics studies and developing novel environmental-friendly virus specific defense strategies in crop protection. In this paper we tested to what extent modern bioinformatics methods are able to reveal new information that would bring us closer to our primary goals. On the date of the search (March 2015) we extracted all available PMMoV entries from publically available databases, represented by heterogeneous data set containing 231 nucleotide sequences covering different parts of the PMMoV genome, that were of different geographical origin, related to different time periods, associated with different pathotypes, and were not previously compared to each other. Results revealed that nucleotide content at genomic positions 552, 565, 639, 666, 708, 5921, 5975 and 6002 can be used to discern three distinct PMMoV genotype variants and their association to one of two virus pathotypes, P-1,P-2 or P-1,P-2,P-3. These sites have never been reported as informative before, probably because by being silent mutations they escaped usual research scrutiny of looking for pathotype determinants among nonsense, missense mutations and indels. Our model was further tested in predicting pathotype of ten newly deposited PMMoV sequences and the successful outcome of the test supported the model as an useful asset for discrimination among pathotypes P-1,P-2 and P-1,P-2,P-3 according to distinct nucleotide content in replicase and coat protein encoding genes. Based on the presented results, we also suggested new tests for fast and cost-effective screening of PMMoV pathotypes and eventually for inducing plant resistance against pepper mild mottle virus.
PB  - Elsevier Sci Ltd, Oxford
T2  - Crop Protection
T1  - Revealing new information from existing genomic data for pepper mild mottle virus pathotype determination
EP  - 103
SP  - 93
VL  - 107
DO  - 10.1016/j.cropro.2018.01.017
ER  - 

@article{
author = "Banović Đeri, Bojana and Pajić, Vesna and Dudić, Dragana",
year = "2018",
abstract = "Primary goals of 21st century science involve eco-friendly solutions for detection, control and suppression of plant viruses. Even though we are accumulating knowledge and data on plant viruses' nucleotide sequences, we are still using a minimum of information available from the collected data. Applying bioinformatics tools and data mining approach to viral sequences is extremely useful in revealing the hidden knowledge, giving guidelines for further biological/bioinformatics studies and developing novel environmental-friendly virus specific defense strategies in crop protection. In this paper we tested to what extent modern bioinformatics methods are able to reveal new information that would bring us closer to our primary goals. On the date of the search (March 2015) we extracted all available PMMoV entries from publically available databases, represented by heterogeneous data set containing 231 nucleotide sequences covering different parts of the PMMoV genome, that were of different geographical origin, related to different time periods, associated with different pathotypes, and were not previously compared to each other. Results revealed that nucleotide content at genomic positions 552, 565, 639, 666, 708, 5921, 5975 and 6002 can be used to discern three distinct PMMoV genotype variants and their association to one of two virus pathotypes, P-1,P-2 or P-1,P-2,P-3. These sites have never been reported as informative before, probably because by being silent mutations they escaped usual research scrutiny of looking for pathotype determinants among nonsense, missense mutations and indels. Our model was further tested in predicting pathotype of ten newly deposited PMMoV sequences and the successful outcome of the test supported the model as an useful asset for discrimination among pathotypes P-1,P-2 and P-1,P-2,P-3 according to distinct nucleotide content in replicase and coat protein encoding genes. Based on the presented results, we also suggested new tests for fast and cost-effective screening of PMMoV pathotypes and eventually for inducing plant resistance against pepper mild mottle virus.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Crop Protection",
title = "Revealing new information from existing genomic data for pepper mild mottle virus pathotype determination",
pages = "103-93",
volume = "107",
doi = "10.1016/j.cropro.2018.01.017"
}

Banović Đeri, B., Pajić, V.,& Dudić, D.. (2018). Revealing new information from existing genomic data for pepper mild mottle virus pathotype determination. in Crop Protection
Elsevier Sci Ltd, Oxford., 107, 93-103.
https://doi.org/10.1016/j.cropro.2018.01.017

Banović Đeri B, Pajić V, Dudić D. Revealing new information from existing genomic data for pepper mild mottle virus pathotype determination. in Crop Protection. 2018;107:93-103.
doi:10.1016/j.cropro.2018.01.017 .

Banović Đeri, Bojana, Pajić, Vesna, Dudić, Dragana, "Revealing new information from existing genomic data for pepper mild mottle virus pathotype determination" in Crop Protection, 107 (2018):93-103,
https://doi.org/10.1016/j.cropro.2018.01.017 . .

TY  - JOUR
AU  - Zhou, Qingwen
AU  - Holloman, William K.
AU  - Kojić, Milorad
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1138
AB  - Primary components of the homologous recombination pathway in eukaryotes include Rad51 whose function is to search for DNA sequence homology and promote strand exchange, its mediator BRCA2, and Dss1, a key regulator of BRCA2. We seek to understand the role of BRCA2 in governing the activity of Rad51 and to learn how BRCA2 function is regulated by Dss1. We use the microbe Ustilago maydis as a model system for experimentation because it has a well-conserved BRCA2-homolog, Brh2, and is amenable to biochemical and molecular genetic manipulations and analysis. The powerful attributes of this system open the way for gaining insight into BRCA2's molecular mechanism through avenues not immediately approachable in the vertebrate systems. Here we provide protocols for preparing Brh2, Dss1, and Rad51 as reagents for use in biochemical assays to monitor function and present methods for transposon-based mutational analysis of Brh2 for use in genetic dissection of function.
PB  - Elsevier Academic Press Inc, San Diego
T2  - Mechanisms of Dna Recombination and Genome Rearrangements: Methods To Study Homologous Recombination
T1  - Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis
EP  - 525
SP  - 513
VL  - 600
DO  - 10.1016/bs.mie.2017.11.019
ER  - 

@article{
author = "Zhou, Qingwen and Holloman, William K. and Kojić, Milorad",
year = "2018",
abstract = "Primary components of the homologous recombination pathway in eukaryotes include Rad51 whose function is to search for DNA sequence homology and promote strand exchange, its mediator BRCA2, and Dss1, a key regulator of BRCA2. We seek to understand the role of BRCA2 in governing the activity of Rad51 and to learn how BRCA2 function is regulated by Dss1. We use the microbe Ustilago maydis as a model system for experimentation because it has a well-conserved BRCA2-homolog, Brh2, and is amenable to biochemical and molecular genetic manipulations and analysis. The powerful attributes of this system open the way for gaining insight into BRCA2's molecular mechanism through avenues not immediately approachable in the vertebrate systems. Here we provide protocols for preparing Brh2, Dss1, and Rad51 as reagents for use in biochemical assays to monitor function and present methods for transposon-based mutational analysis of Brh2 for use in genetic dissection of function.",
publisher = "Elsevier Academic Press Inc, San Diego",
journal = "Mechanisms of Dna Recombination and Genome Rearrangements: Methods To Study Homologous Recombination",
title = "Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis",
pages = "525-513",
volume = "600",
doi = "10.1016/bs.mie.2017.11.019"
}

Zhou, Q., Holloman, W. K.,& Kojić, M.. (2018). Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis. in Mechanisms of Dna Recombination and Genome Rearrangements: Methods To Study Homologous Recombination
Elsevier Academic Press Inc, San Diego., 600, 513-525.
https://doi.org/10.1016/bs.mie.2017.11.019

Zhou Q, Holloman WK, Kojić M. Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis. in Mechanisms of Dna Recombination and Genome Rearrangements: Methods To Study Homologous Recombination. 2018;600:513-525.
doi:10.1016/bs.mie.2017.11.019 .

Zhou, Qingwen, Holloman, William K., Kojić, Milorad, "Approaches to Understanding the Mediator Function of Brh2 in Ustilago maydis" in Mechanisms of Dna Recombination and Genome Rearrangements: Methods To Study Homologous Recombination, 600 (2018):513-525,
https://doi.org/10.1016/bs.mie.2017.11.019 . .

TY  - JOUR
AU  - Selaković, Sara D.
AU  - Stanisavljević, Nemanja
AU  - Vujić, Vukica D.
AU  - Rubinjoni, Luka Z.
AU  - Jovanović, Živko
AU  - Radović, Svetlana R.
AU  - Cvetković, Dragana D.
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1096
AB  - Interactions between plants and herbivorous insects can be shaped by light environment, resulting in differential herbivory in sun and shade. In dioecious species, the combination of plant sex and light-induced changes in defense traits and nutritive value can alter the patterns of foliar damage. We explored the combined effects of light environment and plant sex on natural herbivore damage and plant traits in the dioecious understory forb Mercurialis perennis on Mt. Kopaonik (Serbia). The role of plant traits in predicting the extent of damage was examined as well. Male and female plants from contrasting light environments (shade vs. sun) were analyzed with respect to leaf damage, as well as plant morphological and biochemical traits (size, specific leaf area, carbon-based defensive compounds and nutritional quality). We found prominent differences in herbivore damage between sun and shade conditions, but not between the sexes. Plants from the sun-exposed site had a significantly larger leaf area removed. The specific leaf area co-varied with herbivore damage in an inverse fashion, while leaf nutritional value had a moderate effect. Contrasting light conditions influenced the patterns of intersexual differences in the contents of condensed tannins and soluble proteins, with females exhibiting higher trait values. We found that factors defining risk of consumption were related to plant morphological traits and nutritive value rather than to chemical defenses.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis
EP  - 479
IS  - 3
SP  - 469
VL  - 70
DO  - 10.2298/ABS171207007S
ER  - 

@article{
author = "Selaković, Sara D. and Stanisavljević, Nemanja and Vujić, Vukica D. and Rubinjoni, Luka Z. and Jovanović, Živko and Radović, Svetlana R. and Cvetković, Dragana D.",
year = "2018",
abstract = "Interactions between plants and herbivorous insects can be shaped by light environment, resulting in differential herbivory in sun and shade. In dioecious species, the combination of plant sex and light-induced changes in defense traits and nutritive value can alter the patterns of foliar damage. We explored the combined effects of light environment and plant sex on natural herbivore damage and plant traits in the dioecious understory forb Mercurialis perennis on Mt. Kopaonik (Serbia). The role of plant traits in predicting the extent of damage was examined as well. Male and female plants from contrasting light environments (shade vs. sun) were analyzed with respect to leaf damage, as well as plant morphological and biochemical traits (size, specific leaf area, carbon-based defensive compounds and nutritional quality). We found prominent differences in herbivore damage between sun and shade conditions, but not between the sexes. Plants from the sun-exposed site had a significantly larger leaf area removed. The specific leaf area co-varied with herbivore damage in an inverse fashion, while leaf nutritional value had a moderate effect. Contrasting light conditions influenced the patterns of intersexual differences in the contents of condensed tannins and soluble proteins, with females exhibiting higher trait values. We found that factors defining risk of consumption were related to plant morphological traits and nutritive value rather than to chemical defenses.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis",
pages = "479-469",
number = "3",
volume = "70",
doi = "10.2298/ABS171207007S"
}

Selaković, S. D., Stanisavljević, N., Vujić, V. D., Rubinjoni, L. Z., Jovanović, Ž., Radović, S. R.,& Cvetković, D. D.. (2018). Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 70(3), 469-479.
https://doi.org/10.2298/ABS171207007S

Selaković SD, Stanisavljević N, Vujić VD, Rubinjoni LZ, Jovanović Ž, Radović SR, Cvetković DD. Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis. in Archives of Biological Sciences. 2018;70(3):469-479.
doi:10.2298/ABS171207007S .

Selaković, Sara D., Stanisavljević, Nemanja, Vujić, Vukica D., Rubinjoni, Luka Z., Jovanović, Živko, Radović, Svetlana R., Cvetković, Dragana D., "Light and sex interplay: differential herbivore damage in sun and shade in dioecious Mercurialis perennis" in Archives of Biological Sciences, 70, no. 3 (2018):469-479,
https://doi.org/10.2298/ABS171207007S . .

TY  - JOUR
AU  - Bosnić, Predrag
AU  - Bosnić, Dragana
AU  - Jasnić, Jovana
AU  - Nikolić, Miroslav
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1154
AB  - Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - Silicon mediates sodium transport and partitioning in maize under moderate salt stress
EP  - 687
SP  - 681
VL  - 155
DO  - 10.1016/j.envexpbot.2018.08.018
ER  - 

@article{
author = "Bosnić, Predrag and Bosnić, Dragana and Jasnić, Jovana and Nikolić, Miroslav",
year = "2018",
abstract = "Silicon (Si) is known to alleviate salt stress in various crops; however, the influence of Si on sodium (Na) transport and partitioning at the tissue, cell and organelle levels is poorly understood. Maize (Zea mays L.) hybrid sensitive to salt stress was exposed to moderate salt stress (40 mM NaCl; simulating conditions in salinized agricultural soils) without or with supply of 1.5 mM Si(OH)(4). We investigated the expression of SOS genes encoding Na+ efflux transporter in various root tissues of maize, paralleled by measurements of tissue Na concentration. In addition, subcellular localization of Na (using Na fluorescent dye) within the leaf mesophyll cells was also performed. Silicon supplied plants accumulate less Na in both root apex and cortex, but allocate more Na+ to the leaves via the xylem. This was accompanied by increased expression of ZrnSOS1 and ZmSOS2 in the root apex and cortex facilitating Na+ exclusion, and in the root stele for enhanced Na+ loading into the xylem. Also, Si down-regulated the expression of ZmHKT1 in the root stele, which further decreased Na+ unloading from the xylem. Consequently, Si increased accumulation of Na in leaves, but also enhances sequestration of Na+ into the vacuoles thereby decreasing Na+ accumulation in the chloroplasts. In response to moderate salt stress in maize, Si shifts the typical glycophyte behavior of this species towards that of halophytes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "Silicon mediates sodium transport and partitioning in maize under moderate salt stress",
pages = "687-681",
volume = "155",
doi = "10.1016/j.envexpbot.2018.08.018"
}

Bosnić, P., Bosnić, D., Jasnić, J.,& Nikolić, M.. (2018). Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 155, 681-687.
https://doi.org/10.1016/j.envexpbot.2018.08.018

Bosnić P, Bosnić D, Jasnić J, Nikolić M. Silicon mediates sodium transport and partitioning in maize under moderate salt stress. in Environmental and Experimental Botany. 2018;155:681-687.
doi:10.1016/j.envexpbot.2018.08.018 .

Bosnić, Predrag, Bosnić, Dragana, Jasnić, Jovana, Nikolić, Miroslav, "Silicon mediates sodium transport and partitioning in maize under moderate salt stress" in Environmental and Experimental Botany, 155 (2018):681-687,
https://doi.org/10.1016/j.envexpbot.2018.08.018 . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Savić, Jelena
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Sencanski, Jelena
AU  - Simonović, Mladen
AU  - Radović, Svetlana
AU  - Vinterhalter, Dragan
AU  - Vinterhalter, Branka
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1181
AB  - This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 x 6H(2)O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.
PB  - Springer Heidelberg, Heidelberg
T2  - Acta Physiologiae Plantarum
T1  - Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro
IS  - 6
VL  - 40
DO  - 10.1007/s11738-018-2677-9
ER  - 

@article{
author = "Stanisavljević, Nemanja and Savić, Jelena and Jovanović, Živko and Miljuš-Đukić, Jovanka and Sencanski, Jelena and Simonović, Mladen and Radović, Svetlana and Vinterhalter, Dragan and Vinterhalter, Branka",
year = "2018",
abstract = "This study investigated the role of antioxidant system of Alyssum markgrafii, during long-term exposure to 0.5 or 1 mM NiCl2 x 6H(2)O in vitro. Applied methodology included sample preparation protocol which reduces oxidation of key metabolites along with novel luminescent method and well-established photometric procedures. During 5-week treatments, plants accumulated 1121 and 2470 ppm of Ni2+ respectively, followed by severe growth retardation, chlorophyll degradation and peroxidation of lipids. These effects were more pronounced after 1 mM Ni2+ treatment and additionally accompanied by increased water loss. Activities of luminol-converting peroxidases and glutathione reductase upon 0.5 mM treatment were increased while catalase and superoxide dismutase were diminished. The fact that these two groups of enzymes run in antiparallel might suggest functional redistribution between antioxidant enzymes rather than orchestrated action to prevent oxidative damage. Total antioxidant capacity (TAC) was also increased after 0.5 mM treatment which coincided with increased GR activity and elevated glutathione content indicating this low molecular weight antioxidant as an important factor associated with nickel tolerance. This study also emphasizes the possible important role of luminol-converting peroxidases in nickel hyperaccumulation, although they are not considered as antioxidant enzymes sensu stricto since some of them can also produce reactive oxygen species as well.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Acta Physiologiae Plantarum",
title = "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro",
number = "6",
volume = "40",
doi = "10.1007/s11738-018-2677-9"
}

Stanisavljević, N., Savić, J., Jovanović, Ž., Miljuš-Đukić, J., Sencanski, J., Simonović, M., Radović, S., Vinterhalter, D.,& Vinterhalter, B.. (2018). Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum
Springer Heidelberg, Heidelberg., 40(6).
https://doi.org/10.1007/s11738-018-2677-9

Stanisavljević N, Savić J, Jovanović Ž, Miljuš-Đukić J, Sencanski J, Simonović M, Radović S, Vinterhalter D, Vinterhalter B. Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro. in Acta Physiologiae Plantarum. 2018;40(6).
doi:10.1007/s11738-018-2677-9 .

Stanisavljević, Nemanja, Savić, Jelena, Jovanović, Živko, Miljuš-Đukić, Jovanka, Sencanski, Jelena, Simonović, Mladen, Radović, Svetlana, Vinterhalter, Dragan, Vinterhalter, Branka, "Fingerprinting of the antioxidant status in Alyssum markgrafii shoots during nickel hyperaccumulation in vitro" in Acta Physiologiae Plantarum, 40, no. 6 (2018),
https://doi.org/10.1007/s11738-018-2677-9 . .

TY  - JOUR
AU  - Mitić, Nevena
AU  - Stanisić, Mariana
AU  - Savić, Jelena
AU  - Cosić, Tatjana
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Marin, Marija
AU  - Radović, Svetlana
AU  - Ninković, Slavica
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1139
AB  - Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate
EP  - 1692
IS  - 6
SP  - 1683
VL  - 255
DO  - 10.1007/s00709-018-1250-0
ER  - 

@article{
author = "Mitić, Nevena and Stanisić, Mariana and Savić, Jelena and Cosić, Tatjana and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Marin, Marija and Radović, Svetlana and Ninković, Slavica",
year = "2018",
abstract = "Chenopodium murale L. is an invasive weed species significantly interfering with wheat crop. However, the complete nature of its allelopathic influence on crops is not yet fully understood. In the present study, the focus is made on establishing the relation between plant morphophysiological changes and oxidative stress, induced by allelopathic extract. Phytotoxic medium of C. murale hairy root clone R5 reduced the germination rate (24% less than control value) of wheat cv. Nataa seeds, as well as seedling growth, diminishing shoot and root length significantly, decreased total chlorophyll content, and induced abnormal root gravitropism. The R5 treatment caused cellular structural abnormalities, reflecting on the root and leaf cell shape and organization. These abnormalities mostly included the increased number of mitochondria and reorganization of the vacuolar compartment, changes in nucleus shape, and chloroplast organization and distribution. The most significant structural changes were observed in cell wall in the form of amoeboid protrusions and folds leading to its irregular shape. These structural alterations were accompanied by an oxidative stress in tissues of treated wheat seedlings, reflected as increased level of H2O2 and other ROS molecules, an increase of radical scavenging capacity and total phenolic content. Accordingly, the retardation of wheat seedling growth by C. murale allelochemicals may represent a consequence of complex activity involving both cell structure alteration and physiological processes.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate",
pages = "1692-1683",
number = "6",
volume = "255",
doi = "10.1007/s00709-018-1250-0"
}

Mitić, N., Stanisić, M., Savić, J., Cosić, T., Stanisavljević, N., Miljuš-Đukić, J., Marin, M., Radović, S.,& Ninković, S.. (2018). Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate. in Protoplasma
Springer Wien, Wien., 255(6), 1683-1692.
https://doi.org/10.1007/s00709-018-1250-0

Mitić N, Stanisić M, Savić J, Cosić T, Stanisavljević N, Miljuš-Đukić J, Marin M, Radović S, Ninković S. Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate. in Protoplasma. 2018;255(6):1683-1692.
doi:10.1007/s00709-018-1250-0 .

Mitić, Nevena, Stanisić, Mariana, Savić, Jelena, Cosić, Tatjana, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Marin, Marija, Radović, Svetlana, Ninković, Slavica, "Physiological and cell ultrastructure disturbances in wheat seedlings generated by Chenopodium murale hairy root exudate" in Protoplasma, 255, no. 6 (2018):1683-1692,
https://doi.org/10.1007/s00709-018-1250-0 . .

TY  - JOUR
AU  - Zivković, Jelena
AU  - Savikin, Katarina
AU  - Stanisavljević, Nemanja
AU  - Zdunić, Gordana
AU  - Stanojković, Tatjana
AU  - Samardžić, Jelena
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1133
AB  - Decoctions obtained from dried apple and pear fruits were subjected to in vitro digestion in the presence of a food matrix in order to determine changes in the polyphenol content and antiproliferative activity. The total phenolic and total proanthocyanidins contents were determined using spectrophotometrical methods, and the phenolic compounds were analyzed by RP-HPLC/DAD before and after digestion. Chlorogenic acid and phlorizin dihydrate were the major identified compounds. The addition of a food matrix immediately decreased the contents of individual and total phenolics. After digestion, they were slightly elevated but still lower than in the initial samples. Antiproliferative activity was investigated on human epithelial carcinoma cell line (HeLa), human colon carcinoma (LS174) and human foetal lung fibroblast (MRC-5) cells. The exhibited growth inhibition was not correlated with the content of phenolics in the tested samples, indicating that it could not be explained solely by their content. With the exception of the apple decoction effect on HeLa cells, the digestion process resulted in significant increases in the antiproliferative activity.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion
EP  - 1326
IS  - 12
SP  - 1315
VL  - 83
DO  - 10.2298/JSC180604073Z
ER  - 

@article{
author = "Zivković, Jelena and Savikin, Katarina and Stanisavljević, Nemanja and Zdunić, Gordana and Stanojković, Tatjana and Samardžić, Jelena",
year = "2018",
abstract = "Decoctions obtained from dried apple and pear fruits were subjected to in vitro digestion in the presence of a food matrix in order to determine changes in the polyphenol content and antiproliferative activity. The total phenolic and total proanthocyanidins contents were determined using spectrophotometrical methods, and the phenolic compounds were analyzed by RP-HPLC/DAD before and after digestion. Chlorogenic acid and phlorizin dihydrate were the major identified compounds. The addition of a food matrix immediately decreased the contents of individual and total phenolics. After digestion, they were slightly elevated but still lower than in the initial samples. Antiproliferative activity was investigated on human epithelial carcinoma cell line (HeLa), human colon carcinoma (LS174) and human foetal lung fibroblast (MRC-5) cells. The exhibited growth inhibition was not correlated with the content of phenolics in the tested samples, indicating that it could not be explained solely by their content. With the exception of the apple decoction effect on HeLa cells, the digestion process resulted in significant increases in the antiproliferative activity.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion",
pages = "1326-1315",
number = "12",
volume = "83",
doi = "10.2298/JSC180604073Z"
}

Zivković, J., Savikin, K., Stanisavljević, N., Zdunić, G., Stanojković, T.,& Samardžić, J.. (2018). Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 83(12), 1315-1326.
https://doi.org/10.2298/JSC180604073Z

Zivković J, Savikin K, Stanisavljević N, Zdunić G, Stanojković T, Samardžić J. Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion. in Journal of the Serbian Chemical Society. 2018;83(12):1315-1326.
doi:10.2298/JSC180604073Z .

Zivković, Jelena, Savikin, Katarina, Stanisavljević, Nemanja, Zdunić, Gordana, Stanojković, Tatjana, Samardžić, Jelena, "Chemical composition and antiproliferative potential of dried wild apple and pear tea before and after in vitro simulated digestion" in Journal of the Serbian Chemical Society, 83, no. 12 (2018):1315-1326,
https://doi.org/10.2298/JSC180604073Z . .

TY  - JOUR
AU  - Miljković, Danijela
AU  - Selaković, Sara
AU  - Vujić, Vukica
AU  - Stanisavljević, Nemanja
AU  - Radović, Svetlana
AU  - Cvetković, Dragana
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1147
AB  - Light environments can influence variation in plant morphology, development and susceptibility to herbivores. Our research interest was to investigate the patterns of herbivore damage and developmental stability in dioecious understory forb Mercurialis perennis in contrasting light habitats, located at 1700m a.s.l. on Mt. Kopaonik. Male and female plants from two light habitats, open (a sun-exposed field) and shaded (a spruce forest) were examined with respect to: herbivore damage (percentage of leaf area loss), fluctuating asymetry (FA) as a measurement of developmental stability, plant morphological and, specifically, leaf size traits, as well as biochemical traits relating to nutritional quality and defence, taking into account the possible presence of intersexual differences. Our results show that herbivore damage was significantly higher in open habitat, as well as one out of four univariate FA indices and the multivariate index. Morphological and biochemical traits, apart from defensive compounds, had higher values in the shade, pointing to sun-exposed habitat being more stressful for this species. Intersexual differences were observed for foliar damage, defensive compounds (phenolics and tannins), all leaf size traits, total leaf area, and protein content. Contrasting light habitats affected most of the analysed traits. Both foliar damage and FA were higher in a more stressful habitat; within habitats, no positive correlations were found. Herbivore damage was significantly male biased in open habitat. The analysis of intersexual differences in developmental stability measured by leaf asymmetry levels provided no evidence that female plants were more sensitive to environmental stress.
PB  - Springer Basel Ag, Basel
T2  - Alpine Botany
T1  - Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats
EP  - 206
IS  - 2
SP  - 193
VL  - 128
DO  - 10.1007/s00035-018-0203-8
ER  - 

@article{
author = "Miljković, Danijela and Selaković, Sara and Vujić, Vukica and Stanisavljević, Nemanja and Radović, Svetlana and Cvetković, Dragana",
year = "2018",
abstract = "Light environments can influence variation in plant morphology, development and susceptibility to herbivores. Our research interest was to investigate the patterns of herbivore damage and developmental stability in dioecious understory forb Mercurialis perennis in contrasting light habitats, located at 1700m a.s.l. on Mt. Kopaonik. Male and female plants from two light habitats, open (a sun-exposed field) and shaded (a spruce forest) were examined with respect to: herbivore damage (percentage of leaf area loss), fluctuating asymetry (FA) as a measurement of developmental stability, plant morphological and, specifically, leaf size traits, as well as biochemical traits relating to nutritional quality and defence, taking into account the possible presence of intersexual differences. Our results show that herbivore damage was significantly higher in open habitat, as well as one out of four univariate FA indices and the multivariate index. Morphological and biochemical traits, apart from defensive compounds, had higher values in the shade, pointing to sun-exposed habitat being more stressful for this species. Intersexual differences were observed for foliar damage, defensive compounds (phenolics and tannins), all leaf size traits, total leaf area, and protein content. Contrasting light habitats affected most of the analysed traits. Both foliar damage and FA were higher in a more stressful habitat; within habitats, no positive correlations were found. Herbivore damage was significantly male biased in open habitat. The analysis of intersexual differences in developmental stability measured by leaf asymmetry levels provided no evidence that female plants were more sensitive to environmental stress.",
publisher = "Springer Basel Ag, Basel",
journal = "Alpine Botany",
title = "Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats",
pages = "206-193",
number = "2",
volume = "128",
doi = "10.1007/s00035-018-0203-8"
}

Miljković, D., Selaković, S., Vujić, V., Stanisavljević, N., Radović, S.,& Cvetković, D.. (2018). Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats. in Alpine Botany
Springer Basel Ag, Basel., 128(2), 193-206.
https://doi.org/10.1007/s00035-018-0203-8

Miljković D, Selaković S, Vujić V, Stanisavljević N, Radović S, Cvetković D. Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats. in Alpine Botany. 2018;128(2):193-206.
doi:10.1007/s00035-018-0203-8 .

Miljković, Danijela, Selaković, Sara, Vujić, Vukica, Stanisavljević, Nemanja, Radović, Svetlana, Cvetković, Dragana, "Patterns of herbivore damage, developmental stability, morphological and biochemical traits in female and male Mercurialis perennis in contrasting light habitats" in Alpine Botany, 128, no. 2 (2018):193-206,
https://doi.org/10.1007/s00035-018-0203-8 . .

TY  - JOUR
AU  - Selaković, Sara
AU  - Vujić, Vukica
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Radović, Svetlana
AU  - Cvetković, Dragana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1022
AB  - Plant-herbivore interactions can be mediated by plant apparency, defensive and nutritional quality traits that change through plant ontogeny, resulting in age-specific herbivory. In dioecious species, opposing allocation patterns in defense may lead to sex-biased herbivory. Here, we examine how onto stage and plant sex determine levels of herbivore damage in understory herb Mercurialis perennis under field conditions. We analyzed variation in plant size (height, total leaf area), physical (specific leaf area) and chemical (total phenolic and condensed tannins contents) defense, and nutritional quality (total water, soluble protein and nonstructural carbohydrate contents) during the shift from reproductive to post-reproductive stage. Furthermore, we explored correlations between the analyzed traits and levels of foliar damage. Post-reproductive plants had lower levels of chemical defense, and larger leaf area removed, in spite of having lower nutritive quality. Opposing patterns of intersexual differences were detected in protein and phenolic contents during reproductive stage, while in post-reproductive stage total leaf area was sexually dimorphic. Female-biased herbivory was apparent only after reproduction. Plant size parameters combined with condensed tannins content determined levels of foliar damage during post reproductive stage, while the only trait covarying with herbivory in reproductive stage was total nonstructural carbohydrate content. Our results support claims of optimal defense theory - sensitive stage of reproduction was better defended. We conclude that different combinations of plant traits mediated interactions with herbivores in mature stages. Differences in reproductive allocation between the sexes may not immediately translate into different levels of damage, stressing the need for considering different ontogenetic stages when exploring sex bias in herbivory.
PB  - Elsevier, Amsterdam
T2  - Acta Oecologica-International Journal of Ecology
T1  - Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb
EP  - 190
SP  - 184
VL  - 85
DO  - 10.1016/j.actao.2017.11.006
ER  - 

@article{
author = "Selaković, Sara and Vujić, Vukica and Stanisavljević, Nemanja and Jovanović, Živko and Radović, Svetlana and Cvetković, Dragana",
year = "2017",
abstract = "Plant-herbivore interactions can be mediated by plant apparency, defensive and nutritional quality traits that change through plant ontogeny, resulting in age-specific herbivory. In dioecious species, opposing allocation patterns in defense may lead to sex-biased herbivory. Here, we examine how onto stage and plant sex determine levels of herbivore damage in understory herb Mercurialis perennis under field conditions. We analyzed variation in plant size (height, total leaf area), physical (specific leaf area) and chemical (total phenolic and condensed tannins contents) defense, and nutritional quality (total water, soluble protein and nonstructural carbohydrate contents) during the shift from reproductive to post-reproductive stage. Furthermore, we explored correlations between the analyzed traits and levels of foliar damage. Post-reproductive plants had lower levels of chemical defense, and larger leaf area removed, in spite of having lower nutritive quality. Opposing patterns of intersexual differences were detected in protein and phenolic contents during reproductive stage, while in post-reproductive stage total leaf area was sexually dimorphic. Female-biased herbivory was apparent only after reproduction. Plant size parameters combined with condensed tannins content determined levels of foliar damage during post reproductive stage, while the only trait covarying with herbivory in reproductive stage was total nonstructural carbohydrate content. Our results support claims of optimal defense theory - sensitive stage of reproduction was better defended. We conclude that different combinations of plant traits mediated interactions with herbivores in mature stages. Differences in reproductive allocation between the sexes may not immediately translate into different levels of damage, stressing the need for considering different ontogenetic stages when exploring sex bias in herbivory.",
publisher = "Elsevier, Amsterdam",
journal = "Acta Oecologica-International Journal of Ecology",
title = "Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb",
pages = "190-184",
volume = "85",
doi = "10.1016/j.actao.2017.11.006"
}

Selaković, S., Vujić, V., Stanisavljević, N., Jovanović, Ž., Radović, S.,& Cvetković, D.. (2017). Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb. in Acta Oecologica-International Journal of Ecology
Elsevier, Amsterdam., 85, 184-190.
https://doi.org/10.1016/j.actao.2017.11.006

Selaković S, Vujić V, Stanisavljević N, Jovanović Ž, Radović S, Cvetković D. Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb. in Acta Oecologica-International Journal of Ecology. 2017;85:184-190.
doi:10.1016/j.actao.2017.11.006 .

Selaković, Sara, Vujić, Vukica, Stanisavljević, Nemanja, Jovanović, Živko, Radović, Svetlana, Cvetković, Dragana, "Ontogenetic stage, plant vigor and sex mediate herbivory loads in a dioecious understory herb" in Acta Oecologica-International Journal of Ecology, 85 (2017):184-190,
https://doi.org/10.1016/j.actao.2017.11.006 . .

TY  - JOUR
AU  - Mihailović, Nevena
AU  - Marković, Violeta
AU  - Matić, Ivana Z.
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Trifunović, Snežana
AU  - Joksović, Ljubinka
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1058
AB  - Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.
PB  - Royal Soc Chemistry, Cambridge
T2  - RSC Advances
T1  - Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids
EP  - 8560
IS  - 14
SP  - 8550
VL  - 7
DO  - 10.1039/c6ra28787e
ER  - 

@article{
author = "Mihailović, Nevena and Marković, Violeta and Matić, Ivana Z. and Stanisavljević, Nemanja and Jovanović, Živko and Trifunović, Snežana and Joksović, Ljubinka",
year = "2017",
abstract = "Eight 1,3,4-oxadiazole derivatives containing phenolic acid moieties (7a-h) and eight of their diacylhydrazine precursors (6a-h) were synthesized, characterized using spectroscopic methods and examined by scavenging of stable DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals. The most potent phenolic 1,3,4-oxadiazoles showed better DPPH scavenging activity in comparison with their corresponding diacylhydrazine precursors as a result of participation of both aromatic rings and a 1,3,4-oxadiazole moiety in resonance stabilization of the formed phenoxyl radical. Four diacylhydrazines (6d, 6e, 6g, and 6h) and four 1,3,4-oxadiazoles (7d, 7e, 7g and 7h) with the best DPPH scavenging activity, were chosen for further evaluation of their antioxidant potential through various assays. The investigated compounds exerted pronounced ABTS radical scavenging capacity, moderate to good H2O2 scavenging properties and strong ferric ion reducing capacity. Further in vitro evaluation of the antioxidant properties of the most active compounds demonstrated their protective effects in normal lung fibroblasts MRC-5 against hydrogen peroxide induced oxidative stress. Diacylhydrazine 6h increased two times the activity of glutathione peroxidase in treated cells in comparison with a control sample and did not affect the superoxide dismutase activity.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "RSC Advances",
title = "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids",
pages = "8560-8550",
number = "14",
volume = "7",
doi = "10.1039/c6ra28787e"
}

Mihailović, N., Marković, V., Matić, I. Z., Stanisavljević, N., Jovanović, Ž., Trifunović, S.,& Joksović, L.. (2017). Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances
Royal Soc Chemistry, Cambridge., 7(14), 8550-8560.
https://doi.org/10.1039/c6ra28787e

Mihailović N, Marković V, Matić IZ, Stanisavljević N, Jovanović Ž, Trifunović S, Joksović L. Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids. in RSC Advances. 2017;7(14):8550-8560.
doi:10.1039/c6ra28787e .

Mihailović, Nevena, Marković, Violeta, Matić, Ivana Z., Stanisavljević, Nemanja, Jovanović, Živko, Trifunović, Snežana, Joksović, Ljubinka, "Synthesis and antioxidant activity of 1,3,4-oxadiazoles and their diacylhydrazine precursors derived from phenolic acids" in RSC Advances, 7, no. 14 (2017):8550-8560,
https://doi.org/10.1039/c6ra28787e . .

TY  - JOUR
AU  - Soukup, Milan
AU  - Martinka, Michal
AU  - Bosnić, Dragana
AU  - Caplovicova, Maria
AU  - Elbaum, Rivka
AU  - Lux, Alexander
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1038
AB  - Background and Aims Deposition of silica in plant cell walls improves their mechanical properties and helps plants to withstand various stress conditions. Its mechanism is still not understood and silica-cell wall interactions are elusive. The objective of this study was to investigate the effect of silica deposition on the development and structure of sorghum root endodermis and to identify the cell wall components involved in silicification. MethodsSorghum bicolor seedlings were grown hydroponically with (Si+) or without (Si-) silicon supplementation. Primary roots were used to investigate the transcription of silicon transporters by quantitative RT-PCR. Silica aggregation was induced also under in vitro conditions in detached root segments. The development and architecture of endodermal cell walls were analysed by histochemistry, microscopy and Raman spectroscopy. Water retention capability was compared between silicified and non-silicified roots. Raman spectroscopy analyses of isolated silica aggregates were also carried out. Key Results Active uptake of silicic acid is provided at the root apex, where silicon transporters Lsi1 and Lsi2 are expressed. The locations of silica aggregation are established during the development of tertiary endodermal cell walls, even in the absence of silicon. Silica aggregation takes place in non-lignified spots in the endodermal cell walls, which progressively accumulate silicic acid, and its condensation initiates at arabinoxylan-ferulic acid complexes. Silicification does not support root water retention capability; however, it decreases root growth inhibition imposed by desiccation. Conclusion A model is proposed in which the formation of silica aggregates in sorghum roots is predetermined by a modified cell wall architecture and takes place as governed by endodermal development. The interaction with silica is provided by arabinoxylan-ferulic acid complexes and interferes with further deposition of lignin. Due to contrasting hydrophobicity, silicification and lignification do not represent functionally equivalent modifications of plant cell walls.
PB  - Oxford Univ Press, Oxford
T2  - Annals of Botany
T1  - Formation of silica aggregates in sorghum root endodermis is predetermined by cell wall architecture and development
EP  - 753
IS  - 5
SP  - 739
VL  - 120
DO  - 10.1093/aob/mcx060
ER  - 

@article{
author = "Soukup, Milan and Martinka, Michal and Bosnić, Dragana and Caplovicova, Maria and Elbaum, Rivka and Lux, Alexander",
year = "2017",
abstract = "Background and Aims Deposition of silica in plant cell walls improves their mechanical properties and helps plants to withstand various stress conditions. Its mechanism is still not understood and silica-cell wall interactions are elusive. The objective of this study was to investigate the effect of silica deposition on the development and structure of sorghum root endodermis and to identify the cell wall components involved in silicification. MethodsSorghum bicolor seedlings were grown hydroponically with (Si+) or without (Si-) silicon supplementation. Primary roots were used to investigate the transcription of silicon transporters by quantitative RT-PCR. Silica aggregation was induced also under in vitro conditions in detached root segments. The development and architecture of endodermal cell walls were analysed by histochemistry, microscopy and Raman spectroscopy. Water retention capability was compared between silicified and non-silicified roots. Raman spectroscopy analyses of isolated silica aggregates were also carried out. Key Results Active uptake of silicic acid is provided at the root apex, where silicon transporters Lsi1 and Lsi2 are expressed. The locations of silica aggregation are established during the development of tertiary endodermal cell walls, even in the absence of silicon. Silica aggregation takes place in non-lignified spots in the endodermal cell walls, which progressively accumulate silicic acid, and its condensation initiates at arabinoxylan-ferulic acid complexes. Silicification does not support root water retention capability; however, it decreases root growth inhibition imposed by desiccation. Conclusion A model is proposed in which the formation of silica aggregates in sorghum roots is predetermined by a modified cell wall architecture and takes place as governed by endodermal development. The interaction with silica is provided by arabinoxylan-ferulic acid complexes and interferes with further deposition of lignin. Due to contrasting hydrophobicity, silicification and lignification do not represent functionally equivalent modifications of plant cell walls.",
publisher = "Oxford Univ Press, Oxford",
journal = "Annals of Botany",
title = "Formation of silica aggregates in sorghum root endodermis is predetermined by cell wall architecture and development",
pages = "753-739",
number = "5",
volume = "120",
doi = "10.1093/aob/mcx060"
}

Soukup, M., Martinka, M., Bosnić, D., Caplovicova, M., Elbaum, R.,& Lux, A.. (2017). Formation of silica aggregates in sorghum root endodermis is predetermined by cell wall architecture and development. in Annals of Botany
Oxford Univ Press, Oxford., 120(5), 739-753.
https://doi.org/10.1093/aob/mcx060

Soukup M, Martinka M, Bosnić D, Caplovicova M, Elbaum R, Lux A. Formation of silica aggregates in sorghum root endodermis is predetermined by cell wall architecture and development. in Annals of Botany. 2017;120(5):739-753.
doi:10.1093/aob/mcx060 .

Soukup, Milan, Martinka, Michal, Bosnić, Dragana, Caplovicova, Maria, Elbaum, Rivka, Lux, Alexander, "Formation of silica aggregates in sorghum root endodermis is predetermined by cell wall architecture and development" in Annals of Botany, 120, no. 5 (2017):739-753,
https://doi.org/10.1093/aob/mcx060 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Đedović, Neda
AU  - Stanisavljević, Suzana
AU  - Gašić, Uroš
AU  - Misić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Djordje
AU  - Timotijević, Gordana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1000
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
EP  - 262
SP  - 249
VL  - 37
DO  - 10.1016/j.jff.2017.07.060
ER  - 

@article{
author = "Jevtić, Bojan and Đedović, Neda and Stanisavljević, Suzana and Gašić, Uroš and Misić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Djordje and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
pages = "262-249",
volume = "37",
doi = "10.1016/j.jff.2017.07.060"
}

Jevtić, B., Đedović, N., Stanisavljević, S., Gašić, U., Misić, D., Despotović, J., Samardžić, J., Miljković, D.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtić B, Đedović N, Stanisavljević S, Gašić U, Misić D, Despotović J, Samardžić J, Miljković D, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtić, Bojan, Đedović, Neda, Stanisavljević, Suzana, Gašić, Uroš, Misić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Djordje, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Kostić, Ljiljana
AU  - Nikolić, Nina
AU  - Bosnić, Dragana
AU  - Samardžić, Jelena
AU  - Nikolić, Miroslav
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1029
AB  - Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions
EP  - 455
IS  - 1-2
SP  - 447
VL  - 419
DO  - 10.1007/s11104-017-3364-0
ER  - 

@article{
author = "Kostić, Ljiljana and Nikolić, Nina and Bosnić, Dragana and Samardžić, Jelena and Nikolić, Miroslav",
year = "2017",
abstract = "Although silicon (Si) is known to improve plant growth under low phosphorus (P) conditions, the in planta mechanisms responsible for this effect are still unknown. Here, we investigated the role of Si on P uptake along with the expression of Pi transporters in wheat (Triticum aestivum L.) grown in low P acid soil in comparison with P fertilization and liming. A combined approach was performed including analyses of rhizosphere soil, tissue P content, the expression of the root Pi transporter genes (TaPHT1.1 and TaPHT1.2), and the root exudation of citrate and malate. Supply of Si in a form of Na2SiO3 increased shoot P concentration to an adequate level in the range of P-fertilized plants. Silicon ameliorated low soil pH and high Al3+ comparable to the effect of liming. The in planta effect of Si on up-regulating the expression of TaPHT1.1 and TaPHT1.2 was several fold higher and consequently P uptake doubled compared to both P fertilization and liming. In addition, Si directly stimulated root Pi acquisition by prominently increasing both malate and citrate exudation rate. Application of Si increased root exudation of organic acids that mobilize Pi in the rhizosphere and up-regulated Pi transporters in wheat roots.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions",
pages = "455-447",
number = "1-2",
volume = "419",
doi = "10.1007/s11104-017-3364-0"
}

Kostić, L., Nikolić, N., Bosnić, D., Samardžić, J.,& Nikolić, M.. (2017). Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil
Springer, Dordrecht., 419(1-2), 447-455.
https://doi.org/10.1007/s11104-017-3364-0

Kostić L, Nikolić N, Bosnić D, Samardžić J, Nikolić M. Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions. in Plant and Soil. 2017;419(1-2):447-455.
doi:10.1007/s11104-017-3364-0 .

Kostić, Ljiljana, Nikolić, Nina, Bosnić, Dragana, Samardžić, Jelena, Nikolić, Miroslav, "Silicon increases phosphorus (P) uptake by wheat under low P acid soil conditions" in Plant and Soil, 419, no. 1-2 (2017):447-455,
https://doi.org/10.1007/s11104-017-3364-0 . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Piotti, A.
AU  - Geburek, T.
AU  - Vendramin, G. G.
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1019
AB  - The last resort for conservation of rare tree populations in refugial areas under high risk of climate driven extinction may be ex situ conservation and assisted translocation. Although such actions require detailed knowledge about the spatial scale and heterogeneity of the within-population distribution of genetic diversity, it is still unknown whether fine-scale spatial genetic structure (FSGS) is present in refugial populations of forest trees. In order to address this issue, we carried out the first whole-population genetic characterisation of a small and isolated refugial population of the IUCN red-listed Serbian spruce [Picea omorika (Pan.) Purk.] from the Balkans. All 418 adult individuals were georeferenced and genotyped at nuclear EST-SSRs and at a mitochondrial (mtDNA) locus. Spatial autocorrelation analyses provided only a simplified description of FSGS, which is concordant with findings in wind-pollinated species with limited seed dispersal. However, Bayesian analysis revealed three heterogeneous, highly differentiated (pairwise G' (ST)  gt  0.3), and spatially localised sub-populations showing only partial overlap with the distribution of mtDNA haplotypes. Such complex structure in only 0.34 ha, resulting mainly from historical events, restrictions to gene flow and high local density, was undetected in previous work based on more traditional sampling schemes for population genetics surveys. We demonstrate the usefulness of sampling schemes leaning towards a whole-population genetic characterisation in mining the finest characteristics of FSGS, and argue that our understanding of genetic structuring in highly heterogeneous refugial regions at both macro- and micro-scales is still rather limited and often oversimplified. This has severe implications on conservation of plant biodiversity from these regions in terms of responses to global climate change.
PB  - Springer, Dordrecht
T2  - Conservation Genetics
T1  - Exploring and conserving a "microcosm": whole-population genetic characterization within a refugial area of the endemic, relict conifer Picea omorika
EP  - 788
IS  - 4
SP  - 777
VL  - 18
DO  - 10.1007/s10592-017-0926-x
ER  - 

@article{
author = "Aleksić, Jelena M. and Piotti, A. and Geburek, T. and Vendramin, G. G.",
year = "2017",
abstract = "The last resort for conservation of rare tree populations in refugial areas under high risk of climate driven extinction may be ex situ conservation and assisted translocation. Although such actions require detailed knowledge about the spatial scale and heterogeneity of the within-population distribution of genetic diversity, it is still unknown whether fine-scale spatial genetic structure (FSGS) is present in refugial populations of forest trees. In order to address this issue, we carried out the first whole-population genetic characterisation of a small and isolated refugial population of the IUCN red-listed Serbian spruce [Picea omorika (Pan.) Purk.] from the Balkans. All 418 adult individuals were georeferenced and genotyped at nuclear EST-SSRs and at a mitochondrial (mtDNA) locus. Spatial autocorrelation analyses provided only a simplified description of FSGS, which is concordant with findings in wind-pollinated species with limited seed dispersal. However, Bayesian analysis revealed three heterogeneous, highly differentiated (pairwise G' (ST)  gt  0.3), and spatially localised sub-populations showing only partial overlap with the distribution of mtDNA haplotypes. Such complex structure in only 0.34 ha, resulting mainly from historical events, restrictions to gene flow and high local density, was undetected in previous work based on more traditional sampling schemes for population genetics surveys. We demonstrate the usefulness of sampling schemes leaning towards a whole-population genetic characterisation in mining the finest characteristics of FSGS, and argue that our understanding of genetic structuring in highly heterogeneous refugial regions at both macro- and micro-scales is still rather limited and often oversimplified. This has severe implications on conservation of plant biodiversity from these regions in terms of responses to global climate change.",
publisher = "Springer, Dordrecht",
journal = "Conservation Genetics",
title = "Exploring and conserving a "microcosm": whole-population genetic characterization within a refugial area of the endemic, relict conifer Picea omorika",
pages = "788-777",
number = "4",
volume = "18",
doi = "10.1007/s10592-017-0926-x"
}

Aleksić, J. M., Piotti, A., Geburek, T.,& Vendramin, G. G.. (2017). Exploring and conserving a "microcosm": whole-population genetic characterization within a refugial area of the endemic, relict conifer Picea omorika. in Conservation Genetics
Springer, Dordrecht., 18(4), 777-788.
https://doi.org/10.1007/s10592-017-0926-x

Aleksić JM, Piotti A, Geburek T, Vendramin GG. Exploring and conserving a "microcosm": whole-population genetic characterization within a refugial area of the endemic, relict conifer Picea omorika. in Conservation Genetics. 2017;18(4):777-788.
doi:10.1007/s10592-017-0926-x .

Aleksić, Jelena M., Piotti, A., Geburek, T., Vendramin, G. G., "Exploring and conserving a "microcosm": whole-population genetic characterization within a refugial area of the endemic, relict conifer Picea omorika" in Conservation Genetics, 18, no. 4 (2017):777-788,
https://doi.org/10.1007/s10592-017-0926-x . .

TY  - JOUR
AU  - Aleksić, Jelena M.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/959
AB  - Mitochondrial genomes (mtDNAs or mitogenomes) of seed plants are characterized by a notoriously unstable organization on account of which available so-called universal or consensus primers may fail to fulfil their foreseen function-amplification of various mtDNA regions in a broad range of plant taxa. Thus, the primers developed for groups assumed to have similar organization of their mitogenomes, such as families, may facilitate a broader usage of more variable non-coding portions of these genomes in group members. Using in silico PCR method and six available complete mitogenomes of Fabaceae, it has been demonstrated that only three out of 36 published universal primer and three Medicago sativa-specific primer pairs that amplify various mtDNA regions are suitable for six representatives of the Fabaceae family upon minor modifications, and develop 21 Fabaceae-specific primer pairs for amplification of all 14 cis-splicing introns in genes of NADH subunits (nad genes) which represent the most commonly used noncoding mtDNA regions in various studies in plants. Using the same method and six available complete mitogenomes of representatives of related families Cucurbitaceae, Euphorbiaceae and Rosaceae and a model plant, Arabidopsis thaliana, it has further been demonstrated that applicability of newly developed primer pairs for amplification of nad introns in more or less related taxa was dependent not only on species evolutionary distances but also on their genome sizes. A reported set of 24 primer pairs is a valuable resource which may facilitate a broader usage of mtDNA variability in future studies at both intra- and inter-specific levels in Fabaceae, which is the third largest family of flowering plants rarely studied at the mtDNA level, and in other more or less related taxa.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Family-specific vs. Universal PCR primers for the study of mitochondrial DNA in plants
EP  - 798
IS  - 2
SP  - 777
VL  - 48
DO  - 10.2298/GENSR1602777A
ER  - 

@article{
author = "Aleksić, Jelena M.",
year = "2016",
abstract = "Mitochondrial genomes (mtDNAs or mitogenomes) of seed plants are characterized by a notoriously unstable organization on account of which available so-called universal or consensus primers may fail to fulfil their foreseen function-amplification of various mtDNA regions in a broad range of plant taxa. Thus, the primers developed for groups assumed to have similar organization of their mitogenomes, such as families, may facilitate a broader usage of more variable non-coding portions of these genomes in group members. Using in silico PCR method and six available complete mitogenomes of Fabaceae, it has been demonstrated that only three out of 36 published universal primer and three Medicago sativa-specific primer pairs that amplify various mtDNA regions are suitable for six representatives of the Fabaceae family upon minor modifications, and develop 21 Fabaceae-specific primer pairs for amplification of all 14 cis-splicing introns in genes of NADH subunits (nad genes) which represent the most commonly used noncoding mtDNA regions in various studies in plants. Using the same method and six available complete mitogenomes of representatives of related families Cucurbitaceae, Euphorbiaceae and Rosaceae and a model plant, Arabidopsis thaliana, it has further been demonstrated that applicability of newly developed primer pairs for amplification of nad introns in more or less related taxa was dependent not only on species evolutionary distances but also on their genome sizes. A reported set of 24 primer pairs is a valuable resource which may facilitate a broader usage of mtDNA variability in future studies at both intra- and inter-specific levels in Fabaceae, which is the third largest family of flowering plants rarely studied at the mtDNA level, and in other more or less related taxa.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Family-specific vs. Universal PCR primers for the study of mitochondrial DNA in plants",
pages = "798-777",
number = "2",
volume = "48",
doi = "10.2298/GENSR1602777A"
}

Aleksić, J. M.. (2016). Family-specific vs. Universal PCR primers for the study of mitochondrial DNA in plants. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 48(2), 777-798.
https://doi.org/10.2298/GENSR1602777A

Aleksić JM. Family-specific vs. Universal PCR primers for the study of mitochondrial DNA in plants. in Genetika-Belgrade. 2016;48(2):777-798.
doi:10.2298/GENSR1602777A .

Aleksić, Jelena M., "Family-specific vs. Universal PCR primers for the study of mitochondrial DNA in plants" in Genetika-Belgrade, 48, no. 2 (2016):777-798,
https://doi.org/10.2298/GENSR1602777A . .