TY  - JOUR
AU  - Veljović Jovanović, Sonja
AU  - Kasalica, Bećko
AU  - Miletić, Katarina
AU  - Vidović, Marija
AU  - Šušić, Nikola
AU  - Jeremić, Dejan
AU  - Belča, Ivan
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/18/14265
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2169
AB  - Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency
IS  - 18
SP  - 14265
VL  - 24
DO  - 10.3390/ijms241814265
ER  - 

@article{
author = "Veljović Jovanović, Sonja and Kasalica, Bećko and Miletić, Katarina and Vidović, Marija and Šušić, Nikola and Jeremić, Dejan and Belča, Ivan",
year = "2023",
abstract = "Chloroplast movement rapidly ameliorates the effects of suboptimal light intensity by accumulating along the periclinal cell walls, as well as the effects of excess light by shifting to the anticlinal cell walls. These acclimation responses are triggered by phototropins located at the plasma membrane and chloroplast envelope. Here, we used a recently developed non-invasive system sensitive to very small changes in red light leaf transmittance to perform long-term continuous measurements of dark–light transitions. As a model system, we used variegated Pelargonium zonale leaves containing green sectors (GS) with fully developed chloroplasts and achlorophyllous, white sectors (WS) with undifferentiated plastids, and higher phototropin expression levels. We observed biphasic changes in the red-light transmittance and oscillations triggered by medium intensities of white light, described by a transient peak preceded by a constant decrease in transmittance level. A slight change in red-light transmittance was recorded even in WS. Furthermore, the chloroplast position at lower light intensities affected the rapid light curves, while high light intensity decreased saturated electron transport, maximum quantum efficiency of photosystem II, and increased non-photochemical quenching of chlorophyll fluorescence and epidermal flavonoids. Our results extend the knowledge of light-dependent chloroplast movements and thus contribute to a better understanding of their role in regulating photosynthesis under fluctuating light conditions.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency",
number = "18",
pages = "14265",
volume = "24",
doi = "10.3390/ijms241814265"
}

Veljović Jovanović, S., Kasalica, B., Miletić, K., Vidović, M., Šušić, N., Jeremić, D.,& Belča, I.. (2023). Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences, 24(18), 14265.
https://doi.org/10.3390/ijms241814265

Veljović Jovanović S, Kasalica B, Miletić K, Vidović M, Šušić N, Jeremić D, Belča I. Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency. in International Journal of Molecular Sciences. 2023;24(18):14265.
doi:10.3390/ijms241814265 .

Veljović Jovanović, Sonja, Kasalica, Bećko, Miletić, Katarina, Vidović, Marija, Šušić, Nikola, Jeremić, Dejan, Belča, Ivan, "Red-Light Transmittance Changes in Variegated Pelargonium zonale—Diurnal Variation in Chloroplast Movement and Photosystem II Efficiency" in International Journal of Molecular Sciences, 24, no. 18 (2023):14265,
https://doi.org/10.3390/ijms241814265 . .

TY  - JOUR
AU  - Milić, Dejana
AU  - Živanović, Bojana
AU  - Samardžić, Jelena
AU  - Nikolić, Nenad
AU  - Cukier, Caroline
AU  - Limami, Anis M.
AU  - Vidović, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1712
AB  - Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance between the amount of absorbed energy and their ability to process it. Variegated leaves with photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue (WL) are an excellent model system to study source–sink interactions within the same leaf under the same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE) conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved maximum efficiency of photosystem II (ФPSII) at the end of the experiment. GL of the EEE-treated plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine, asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides. Thus, WL acts as an important energy escape valve that is required in order to maintain the successful performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of variegation, as no consistent conclusions about its ecological benefits have been proposed so far.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions
IS  - 3
SP  - 2269
VL  - 24
DO  - 10.3390/ijms24032269
ER  - 

@article{
author = "Milić, Dejana and Živanović, Bojana and Samardžić, Jelena and Nikolić, Nenad and Cukier, Caroline and Limami, Anis M. and Vidović, Marija",
year = "2023",
abstract = "Plants are inevitably exposed to extreme climatic conditions that lead to a disturbed balance between the amount of absorbed energy and their ability to process it. Variegated leaves with photosynthetically active green leaf tissue (GL) and photosynthetically inactive white leaf tissue (WL) are an excellent model system to study source–sink interactions within the same leaf under the same microenvironmental conditions. We demonstrated that under excess excitation energy (EEE) conditions (high irradiance and lower temperature), regulated metabolic reprogramming in both leaf tissues allowed an increased consumption of reducing equivalents, as evidenced by preserved maximum efficiency of photosystem II (ФPSII) at the end of the experiment. GL of the EEE-treated plants employed two strategies: (i) the accumulation of flavonoid glycosides, especially cyanidin glycosides, as an alternative electron sink, and (ii) cell wall stiffening by cellulose, pectin, and lignin accumulation. On the other hand, WL increased the amount of free amino acids, mainly arginine, asparagine, branched-chain and aromatic amino acids, as well as kaempferol and quercetin glycosides. Thus, WL acts as an important energy escape valve that is required in order to maintain the successful performance of the GL sectors under EEE conditions. Finally, this role could be an adaptive value of variegation, as no consistent conclusions about its ecological benefits have been proposed so far.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions",
number = "3",
pages = "2269",
volume = "24",
doi = "10.3390/ijms24032269"
}

Milić, D., Živanović, B., Samardžić, J., Nikolić, N., Cukier, C., Limami, A. M.,& Vidović, M.. (2023). Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences, 24(3), 2269.
https://doi.org/10.3390/ijms24032269

Milić D, Živanović B, Samardžić J, Nikolić N, Cukier C, Limami AM, Vidović M. Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions. in International Journal of Molecular Sciences. 2023;24(3):2269.
doi:10.3390/ijms24032269 .

Milić, Dejana, Živanović, Bojana, Samardžić, Jelena, Nikolić, Nenad, Cukier, Caroline, Limami, Anis M., Vidović, Marija, "Carbon and Nitrogen Allocation between the Sink and Source Leaf Tissue in Response to the Excess Excitation Energy Conditions" in International Journal of Molecular Sciences, 24, no. 3 (2023):2269,
https://doi.org/10.3390/ijms24032269 . .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Veljović Jovanović, Sonja
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1867
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods
without water, enter a state of anhydrobiosis, and fully recover upon water arrival.
Ramonda serbica is a relic and endemic species that belong to a very small group of
desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental
conditions have been an appealing subject to many researchers. Most of the genes
responsible for this amazing ability are present in other plants, and this path of research
where those genes could be activated in crops is growing much more attention because of
the imminent crisis regarding food supplies in the near future. Key components involved in
the response to dehydration in R. serbica plants were analysed through a comprehensive
transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis
abundant proteins play a significant role in the complex defence processes involved in
desiccation tolerance. Defining physicochemical characteristics and specific physiological
functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability
in other areas of research.
PB  - Belgrade : Faculty of Chemistry, Serbian Biochemical Societ
C3  - Amazing Biochemistry
T1  - Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein
SP  - 37
VL  - 11
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1867
ER  - 

@conference{
author = "Milić Komić, Sonja and Veljović Jovanović, Sonja and Pantelić, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods
without water, enter a state of anhydrobiosis, and fully recover upon water arrival.
Ramonda serbica is a relic and endemic species that belong to a very small group of
desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental
conditions have been an appealing subject to many researchers. Most of the genes
responsible for this amazing ability are present in other plants, and this path of research
where those genes could be activated in crops is growing much more attention because of
the imminent crisis regarding food supplies in the near future. Key components involved in
the response to dehydration in R. serbica plants were analysed through a comprehensive
transcriptomic, proteomic, metabolite and photosynthetic study. Late embryogenesis
abundant proteins play a significant role in the complex defence processes involved in
desiccation tolerance. Defining physicochemical characteristics and specific physiological
functions of late embryogenesis abundant proteins – LEAPs may lead to their applicability
in other areas of research.",
publisher = "Belgrade : Faculty of Chemistry, Serbian Biochemical Societ",
journal = "Amazing Biochemistry",
title = "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein",
pages = "37",
volume = "11",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1867"
}

Milić Komić, S., Veljović Jovanović, S., Pantelić, A.,& Vidović, M.. (2022). Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Amazing Biochemistry
Belgrade : Faculty of Chemistry, Serbian Biochemical Societ., 11, 37.
https://hdl.handle.net/21.15107/rcub_imagine_1867

Milić Komić S, Veljović Jovanović S, Pantelić A, Vidović M. Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein. in Amazing Biochemistry. 2022;11:37.
https://hdl.handle.net/21.15107/rcub_imagine_1867 .

Milić Komić, Sonja, Veljović Jovanović, Sonja, Pantelić, Ana, Vidović, Marija, "Late embryogenesis abundant proteins: Structural characterisation and interaction with α-synuclein" in Amazing Biochemistry, 11 (2022):37,
https://hdl.handle.net/21.15107/rcub_imagine_1867 .

TY  - CONF
AU  - Nikolić, Ljiljana
AU  - Vidović, Marija
AU  - Todorović, Nataša
AU  - Petković, Branka
AU  - Stojadinović, Gordana
AU  - Martać, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2168
AB  - Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.
PB  - Belgrade : Society of Physical Chemists of Serbia
C3  - Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia
T1  - Protoplast patch-clamping using an upright microscope with a movable stage
EP  - 274
SP  - 271
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5508
ER  - 

@conference{
editor = "Čupić, Željko, Anić, Slobodan",
author = "Nikolić, Ljiljana and Vidović, Marija and Todorović, Nataša and Petković, Branka and Stojadinović, Gordana and Martać, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success ofexperiments is highly dependent on the possibility to isolate metabolically active protoplasts thatcan withstand membrane current recordings. The aim of the present work is to obtain viableprotoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patchclamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplastswith preserved membrane integrity suitable for electrophysiological experiments. We applied acustom approach for patch-clamping protoplasts using a microscope with a movable microscopestage. We recorded prominent inward and prominent outward types of membrane current profiles ofprotoplasts. Obtained data indicate that optimized isolation protocol and custom system for patchclamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Belgrade : Society of Physical Chemists of Serbia",
journal = "Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia",
title = "Protoplast patch-clamping using an upright microscope with a movable stage",
pages = "274-271",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5508"
}

Čupić, Ž., Anić, S., Nikolić, L., Vidović, M., Todorović, N., Petković, B., Stojadinović, G., Martać, L.,& Bogdanović Pristov, J.. (2022). Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia
Belgrade : Society of Physical Chemists of Serbia., 271-274.
https://hdl.handle.net/21.15107/rcub_ibiss_5508

Čupić Ž, Anić S, Nikolić L, Vidović M, Todorović N, Petković B, Stojadinović G, Martać L, Bogdanović Pristov J. Protoplast patch-clamping using an upright microscope with a movable stage. in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia. 2022;:271-274.
https://hdl.handle.net/21.15107/rcub_ibiss_5508 .

Čupić, Željko, Anić, Slobodan, Nikolić, Ljiljana, Vidović, Marija, Todorović, Nataša, Petković, Branka, Stojadinović, Gordana, Martać, Ljiljana, Bogdanović Pristov, Jelena, "Protoplast patch-clamping using an upright microscope with a movable stage" in Proceedings: Physical Chemistry 2022, Vol. 1.: 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry; 2022 Sep 26-30; Belgrade, Serbia (2022):271-274,
https://hdl.handle.net/21.15107/rcub_ibiss_5508 .

TY  - CONF
AU  - Živanović, Bojana
AU  - Prokić, Ljiljana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1842
AB  - The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1842
ER  - 

@conference{
author = "Živanović, Bojana and Prokić, Ljiljana and Milić Komić, Sonja and Nikolić, Nenad and Sedlarević Zorić, Ana and Vidović, Marija and Veljović Jovanović, Sonja",
year = "2022",
abstract = "The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1842"
}

Živanović, B., Prokić, L., Milić Komić, S., Nikolić, N., Sedlarević Zorić, A., Vidović, M.,& Veljović Jovanović, S.. (2022). Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 83.
https://hdl.handle.net/21.15107/rcub_imagine_1842

Živanović B, Prokić L, Milić Komić S, Nikolić N, Sedlarević Zorić A, Vidović M, Veljović Jovanović S. Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:83.
https://hdl.handle.net/21.15107/rcub_imagine_1842 .

Živanović, Bojana, Prokić, Ljiljana, Milić Komić, Sonja, Nikolić, Nenad, Sedlarević Zorić, Ana, Vidović, Marija, Veljović Jovanović, Sonja, "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca" in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):83,
https://hdl.handle.net/21.15107/rcub_imagine_1842 .

TY  - JOUR
AU  - Pantelić, Ana
AU  - Stevanović, Strahinja
AU  - Komić, Sonja Milic
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1560
AB  - Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic alpha-helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation-upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic alpha-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc
IS  - 7
VL  - 23
DO  - 10.3390/ijms23073547
ER  - 

@article{
author = "Pantelić, Ana and Stevanović, Strahinja and Komić, Sonja Milic and Kilibarda, Nataša and Vidović, Marija",
year = "2022",
abstract = "Ramonda serbica Panc. is an ancient resurrection plant able to survive a long desiccation period and recover metabolic functions upon watering. The accumulation of protective late embryogenesis abundant proteins (LEAPs) is a desiccation tolerance hallmark. To propose their role in R. serbica desiccation tolerance, we structurally characterised LEAPs and evaluated LEA gene expression levels in hydrated and desiccated leaves. By integrating de novo transcriptomics and homologues LEAP domains, 318 R. serbica LEAPs were identified and classified according to their conserved motifs and phylogeny. The in silico analysis revealed that hydrophilic LEA4 proteins exhibited an exceptionally high tendency to form amphipathic alpha-helices. The most abundant, atypical LEA2 group contained more hydrophobic proteins predicted to fold into the defined globular domains. Within the desiccation-upregulated LEA genes, the majority encoded highly disordered DEH1, LEA1, LEA4.2, and LEA4.3 proteins, while the greatest portion of downregulated genes encoded LEA2.3 and LEA2.5 proteins. While dehydrins might chelate metals and bind DNA under water deficit, other intrinsically disordered LEAPs might participate in forming intracellular proteinaceous condensates or adopt amphipathic alpha-helical conformation, enabling them to stabilise desiccation-sensitive proteins and membranes. This comprehensive LEAPs structural characterisation is essential to understanding their function and regulation during desiccation aiming at crop drought tolerance improvement.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc",
number = "7",
volume = "23",
doi = "10.3390/ijms23073547"
}

Pantelić, A., Stevanović, S., Komić, S. M., Kilibarda, N.,& Vidović, M.. (2022). In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc. in International Journal of Molecular Sciences
MDPI, Basel., 23(7).
https://doi.org/10.3390/ijms23073547

Pantelić A, Stevanović S, Komić SM, Kilibarda N, Vidović M. In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc. in International Journal of Molecular Sciences. 2022;23(7).
doi:10.3390/ijms23073547 .

Pantelić, Ana, Stevanović, Strahinja, Komić, Sonja Milic, Kilibarda, Nataša, Vidović, Marija, "In Silico Characterisation of the Late Embryogenesis Abundant (LEA) Protein Families and Their Role in Desiccation Tolerance in Ramonda serbica Panc" in International Journal of Molecular Sciences, 23, no. 7 (2022),
https://doi.org/10.3390/ijms23073547 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Veljković Jovanović, Sonja
AU  - Pantelić, Ana
AU  - Vidović, Marija
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1775
AB  - Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter
a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species
that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing
subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and
research of those genes which could be activated in crops is growing much more attention because of the imminent
crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica
plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study.
Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation
tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their
applicability in other areas of research.
PB  - Novi Sad : Faculty of Sciences, Department of Biology
T2  - Biologia Serbica
T1  - Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.
EP  - 66
IS  - 1
SP  - 59
VL  - 41
DO  - 10.5281/zenodo.7075212
ER  - 

@article{
author = "Milić Komić, Sonja and Veljković Jovanović, Sonja and Pantelić, Ana and Vidović, Marija",
year = "2022",
abstract = "Ressurection plants are extraordinary because of their ability to withstand long periods without water, enter
a state of anhydrobiosis, and fully recover upon water arrival. Ramonda serbica Panč. is a relic and endemic species
that belongs to a very small group of desiccation-tolerant plants in Europe. Underlying physiological, molecular and
morphological mechanisms that enable these plants to survive harsh environmental conditions have been an appealing
subject of many researchers. Most of the genes responsible for this amazing ability are present in other plants, and
research of those genes which could be activated in crops is growing much more attention because of the imminent
crisis regarding food supplies in the near future. Key components involved in the response to dehydration in R. serbica
plants were analysed through a comprehensive transcriptomic, proteomic, metabolite and photosynthetic study.
Late embryogenesis abundant proteins play a significant role in the complex defence processes involved in desiccation
tolerance. Defining LEAPs physicochemical characteristics and specific physiological functions may lead us to their
applicability in other areas of research.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology",
journal = "Biologia Serbica",
title = "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.",
pages = "66-59",
number = "1",
volume = "41",
doi = "10.5281/zenodo.7075212"
}

Milić Komić, S., Veljković Jovanović, S., Pantelić, A.,& Vidović, M.. (2022). Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology., 41(1), 59-66.
https://doi.org/10.5281/zenodo.7075212

Milić Komić S, Veljković Jovanović S, Pantelić A, Vidović M. Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč.. in Biologia Serbica. 2022;41(1):59-66.
doi:10.5281/zenodo.7075212 .

Milić Komić, Sonja, Veljković Jovanović, Sonja, Pantelić, Ana, Vidović, Marija, "Structural characterisation of late embryogenesis abundant proteins in Ramonda serbica Panč." in Biologia Serbica, 41, no. 1 (2022):59-66,
https://doi.org/10.5281/zenodo.7075212 . .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Živanović, Bojana
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1841
AB  - Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimumbasilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent themost abundant class of secondary metabolites, and their function in plants involves protectionagainst numerous abiotic stresses, role in growth and development, flowering, reproduction andseed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activityand decreased ascorbate content were characteristic responses to pro-oxidative effect of the highUV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulationof epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc andGSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that weused as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transferfrom initial 50 to 250 µmol photons m-2s-1.
PB  - Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting
T1  - Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1841
ER  - 

@conference{
author = "Milić Komić, Sonja and Živanović, Bojana and Sedlarević Zorić, Ana and Vidović, Marija and Veljović Jovanović, Sonja",
year = "2022",
abstract = "Three varieties of basil (Ocimum x citriodorum, Ocimum basilicum var. Genovese and Ocimumbasilicum var. purpurascens) were used to examine the effect of different PAR intensities (100, 400and 1400 µmol photons m-2s-1), as well as different UV-B/PAR ratios, on leaf phenolics accumulation, and components of the ascorbate-glutathione cycle. Phenolic compounds represent themost abundant class of secondary metabolites, and their function in plants involves protectionagainst numerous abiotic stresses, role in growth and development, flowering, reproduction andseed dispersion. A preferential accumulation of HCAs over Flav, induction of class III POD activityand decreased ascorbate content were characteristic responses to pro-oxidative effect of the highUV-B/PAR ratio in all three basil varieties. The most remarkable result was the lack of accumulationof epidermal UV absorbing substances, which appeared to be a crucial photoprotective mechanism in sunlight. The contrasting effect of ecologically relevant UV-B radiation on basil plants,pro-oxidative vs. acclimative, was determined by the quality and intensity of the background light.Acclimation of basil to high light comprises a number of processes, among which are the accumulation of epidermal flavonoids and total leaf phenolics, antioxidant response (increase in Asc andGSH) and lack of downregulation of PSII upon increasing light intensity. Among varieties that weused as model system in this study, purple basil, with the highest constitutive amount of anthocyanidins, showed the least induction of epidermal flavonoids and lack of light effect upon transferfrom initial 50 to 250 µmol photons m-2s-1.",
publisher = "Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting",
title = "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1841"
}

Milić Komić, S., Živanović, B., Sedlarević Zorić, A., Vidović, M.,& Veljović Jovanović, S.. (2022). Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology and 23rd SPPS Meeting
Serbian Plant Physiology Society  Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia,  University of Belgrade  Faculty of Biology, University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_imagine_1841

Milić Komić S, Živanović B, Sedlarević Zorić A, Vidović M, Veljović Jovanović S. Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties. in 4th International Conference on Plant Biology and 23rd SPPS Meeting. 2022;:43.
https://hdl.handle.net/21.15107/rcub_imagine_1841 .

Milić Komić, Sonja, Živanović, Bojana, Sedlarević Zorić, Ana, Vidović, Marija, Veljović Jovanović, Sonja, "Distinctive regulation of different phenolics biosynthesis by high light and UV-B in three basil varieties" in 4th International Conference on Plant Biology and 23rd SPPS Meeting (2022):43,
https://hdl.handle.net/21.15107/rcub_imagine_1841 .

TY  - JOUR
AU  - Perić, Mina
AU  - Bataveljić, Danijela
AU  - Bijelić, Dunja
AU  - Milicević, Katarina
AU  - Andjus, Pavle R.
AU  - Pristov, Jelena Bogdanovic
AU  - Nikolić, Ljiljana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1531
AB  - We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.
PB  - Wiley, Hoboken
T2  - Microscopy Research and Technique
T1  - Approach for patch-clamping using an upright microscope with z-axis movable stage
EP  - 2104
IS  - 6
SP  - 2095
VL  - 85
DO  - 10.1002/jemt.24066
ER  - 

@article{
author = "Perić, Mina and Bataveljić, Danijela and Bijelić, Dunja and Milicević, Katarina and Andjus, Pavle R. and Pristov, Jelena Bogdanovic and Nikolić, Ljiljana",
year = "2022",
abstract = "We describe an approach for studying the physiology of single live cells using the conceptionally novel upright microscope/patch-clamp configuration. Electrophysiology experiments typically require a microscope with the fixed stage position and the motion control of the microscope objective. Here, we demonstrate that a microscope with a z-axis movable stage and a fixed objective can also be efficiently used in combination with the patch-clamp technique. We define a set of underlying principles governing the operation of this microscope/patch-clamp configuration and demonstrate its performance in practice using cultured astrocytes, microglia, and oligodendrocytes. Experimental results show that our custom configuration provides stable recordings, has a high success rate of the whole-cell patch-clamp trials, can be effectively applied to study cellular physiology of glial cells, and provides comparable performance and usability to the commercially available systems. Our system can be easily replicated or adapted to suit the needs of the research groups and can be cost-effective in reducing the investments in purchasing additional equipment. We provide step-by-step instructions on implementing an upright microscope with z-axis movable stage as a routine workhorse for patch-clamping.",
publisher = "Wiley, Hoboken",
journal = "Microscopy Research and Technique",
title = "Approach for patch-clamping using an upright microscope with z-axis movable stage",
pages = "2104-2095",
number = "6",
volume = "85",
doi = "10.1002/jemt.24066"
}

Perić, M., Bataveljić, D., Bijelić, D., Milicević, K., Andjus, P. R., Pristov, J. B.,& Nikolić, L.. (2022). Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique
Wiley, Hoboken., 85(6), 2095-2104.
https://doi.org/10.1002/jemt.24066

Perić M, Bataveljić D, Bijelić D, Milicević K, Andjus PR, Pristov JB, Nikolić L. Approach for patch-clamping using an upright microscope with z-axis movable stage. in Microscopy Research and Technique. 2022;85(6):2095-2104.
doi:10.1002/jemt.24066 .

Perić, Mina, Bataveljić, Danijela, Bijelić, Dunja, Milicević, Katarina, Andjus, Pavle R., Pristov, Jelena Bogdanovic, Nikolić, Ljiljana, "Approach for patch-clamping using an upright microscope with z-axis movable stage" in Microscopy Research and Technique, 85, no. 6 (2022):2095-2104,
https://doi.org/10.1002/jemt.24066 . .

TY  - CHAP
AU  - Vidović, Marija
AU  - Komić Milić, Sonja
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1776
UR  - https://novapublishers.com/shop/a-closer-look-at-proteolysis/
AB  - Proteolysis represents a primary recycling system for amino acids.
Moreover, by controlling the protein turnover, proteolysis plays an
important role in key cellular processes such as control of cell cycle,
programmed cell death (including senescence) and response to various
stimuli. In eukaryotic cells, most proteins are degraded by autophagy and
26S proteasome machinery, composed in general of 20S proteolytic core and 19S regulatory particle. The protein susceptibility to proteolysis is
determined by its structural features. Folded globular proteins in their
native state are rarely degraded, however when misfolded, denatured or
when an unstructured region is attached, the degradation is promoted.
Proteins natively containing intrinsically disordered regions (IDRs) or
completely lacking the stable secondary and tertiary structures are
defined as intrinsically disordered proteins (IDPs). Due to their high
intramolecular flexibility and plasticity, IDPs are involved in DNA
metabolism, transcriptional activation, autophagy, and signalling
cascades related to response to various stimuli. In sessile organisms such
as plants, IDPs enable prompt acclimation to external factors, including
light perception, adaptation to oxidative stress and water loss, and
regulation of protective, antioxidative and secondary metabolism.
Binding of specific ligands and partners to particular IDP triggers
structural changes and affects the stability of IDP, its susceptibility to
proteolysis and aggregation-propensity. Theabnormal aggregation of
several IDPs and altered proteolysis pathways are closely connected with
serious neurodegenerative disorders, such as Alzheimer’s and Parkinson's
diseases. In this chapter, we discuss the current understanding of
proteolytic processes of specific, well-characterised IDPs under different
physiological states, emphasizing the influence of the microenvironment
and ligands/partners on their conformation.
PB  - Nova Science Publishers, Inc.
T2  - A closer look at proteolysis
T1  - Regulation of proteolysis of intrinsically disordered proteins: physiological consequences
EP  - 46
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1776
ER  - 

@inbook{
author = "Vidović, Marija and Komić Milić, Sonja",
year = "2021",
abstract = "Proteolysis represents a primary recycling system for amino acids.
Moreover, by controlling the protein turnover, proteolysis plays an
important role in key cellular processes such as control of cell cycle,
programmed cell death (including senescence) and response to various
stimuli. In eukaryotic cells, most proteins are degraded by autophagy and
26S proteasome machinery, composed in general of 20S proteolytic core and 19S regulatory particle. The protein susceptibility to proteolysis is
determined by its structural features. Folded globular proteins in their
native state are rarely degraded, however when misfolded, denatured or
when an unstructured region is attached, the degradation is promoted.
Proteins natively containing intrinsically disordered regions (IDRs) or
completely lacking the stable secondary and tertiary structures are
defined as intrinsically disordered proteins (IDPs). Due to their high
intramolecular flexibility and plasticity, IDPs are involved in DNA
metabolism, transcriptional activation, autophagy, and signalling
cascades related to response to various stimuli. In sessile organisms such
as plants, IDPs enable prompt acclimation to external factors, including
light perception, adaptation to oxidative stress and water loss, and
regulation of protective, antioxidative and secondary metabolism.
Binding of specific ligands and partners to particular IDP triggers
structural changes and affects the stability of IDP, its susceptibility to
proteolysis and aggregation-propensity. Theabnormal aggregation of
several IDPs and altered proteolysis pathways are closely connected with
serious neurodegenerative disorders, such as Alzheimer’s and Parkinson's
diseases. In this chapter, we discuss the current understanding of
proteolytic processes of specific, well-characterised IDPs under different
physiological states, emphasizing the influence of the microenvironment
and ligands/partners on their conformation.",
publisher = "Nova Science Publishers, Inc.",
journal = "A closer look at proteolysis",
booktitle = "Regulation of proteolysis of intrinsically disordered proteins: physiological consequences",
pages = "46-1",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1776"
}

Vidović, M.,& Komić Milić, S.. (2021). Regulation of proteolysis of intrinsically disordered proteins: physiological consequences. in A closer look at proteolysis
Nova Science Publishers, Inc.., 1-46.
https://hdl.handle.net/21.15107/rcub_imagine_1776

Vidović M, Komić Milić S. Regulation of proteolysis of intrinsically disordered proteins: physiological consequences. in A closer look at proteolysis. 2021;:1-46.
https://hdl.handle.net/21.15107/rcub_imagine_1776 .

Vidović, Marija, Komić Milić, Sonja, "Regulation of proteolysis of intrinsically disordered proteins: physiological consequences" in A closer look at proteolysis (2021):1-46,
https://hdl.handle.net/21.15107/rcub_imagine_1776 .

TY  - CONF
AU  - Marković, Nemanja
AU  - Milić Komić, Sonja
AU  - Radosavljević, Jelena
AU  - Pantelić, Ana
AU  - Kilibarda, Nataša
AU  - Vidović, Marija
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1870
AB  - Late embryogenesis abundant (LEA) proteins are induced in cellular dehydration, such as
freezing, drought, or desiccation. They can be involved in antioxidative defense, ion
sequestration, and structural stabilization of both membranes and enzymes during freezing
or drying, while by forming intracellular proteinaceous condensates they increase
structural integrity and intracellular viscosity of cells during desiccation 1. The genome of
the model plant Arabidopsis thaliana contains 51 genes encoding LEA proteins2. The
majority of these LEA proteins (35%) belongs to Pfam LEA_4 (PF02987) family. In silico
analysis suggested that these proteins are highly hydrophilic proteins with significant
intrinsically disordered protein (IDP) properties. In order to evaluate structural properties
and possible functions of LEA_4 protein family under different water content, a
representative AtLEA25 protein (At2g42560, 635 aa), naturally located in the cytoplasm
of seeds3 was obtained in Escherichia coli by recombinant DNA technology. Although this
technology has been traditionally used to over-express and purify various globular
proteins, numerous reports have shown that the IDPs, due to their structural plasicity are
naturally highly susceptible to proteolytic cleavage. To conduct structural and functional
studies we developed a robust method to produce highly purified (>95% pure) AtLEA25
with no detectable amount of protein breakdown products.
PB  - Belgrade : Serbian Biochemical Society
C3  - Biochemical Insights into Molecular Mechanisms
T1  - Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology
EP  - 99
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1870
ER  - 

@conference{
author = "Marković, Nemanja and Milić Komić, Sonja and Radosavljević, Jelena and Pantelić, Ana and Kilibarda, Nataša and Vidović, Marija",
year = "2021",
abstract = "Late embryogenesis abundant (LEA) proteins are induced in cellular dehydration, such as
freezing, drought, or desiccation. They can be involved in antioxidative defense, ion
sequestration, and structural stabilization of both membranes and enzymes during freezing
or drying, while by forming intracellular proteinaceous condensates they increase
structural integrity and intracellular viscosity of cells during desiccation 1. The genome of
the model plant Arabidopsis thaliana contains 51 genes encoding LEA proteins2. The
majority of these LEA proteins (35%) belongs to Pfam LEA_4 (PF02987) family. In silico
analysis suggested that these proteins are highly hydrophilic proteins with significant
intrinsically disordered protein (IDP) properties. In order to evaluate structural properties
and possible functions of LEA_4 protein family under different water content, a
representative AtLEA25 protein (At2g42560, 635 aa), naturally located in the cytoplasm
of seeds3 was obtained in Escherichia coli by recombinant DNA technology. Although this
technology has been traditionally used to over-express and purify various globular
proteins, numerous reports have shown that the IDPs, due to their structural plasicity are
naturally highly susceptible to proteolytic cleavage. To conduct structural and functional
studies we developed a robust method to produce highly purified (>95% pure) AtLEA25
with no detectable amount of protein breakdown products.",
publisher = "Belgrade : Serbian Biochemical Society",
journal = "Biochemical Insights into Molecular Mechanisms",
title = "Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology",
pages = "99-98",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1870"
}

Marković, N., Milić Komić, S., Radosavljević, J., Pantelić, A., Kilibarda, N.,& Vidović, M.. (2021). Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology. in Biochemical Insights into Molecular Mechanisms
Belgrade : Serbian Biochemical Society., 98-99.
https://hdl.handle.net/21.15107/rcub_imagine_1870

Marković N, Milić Komić S, Radosavljević J, Pantelić A, Kilibarda N, Vidović M. Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology. in Biochemical Insights into Molecular Mechanisms. 2021;:98-99.
https://hdl.handle.net/21.15107/rcub_imagine_1870 .

Marković, Nemanja, Milić Komić, Sonja, Radosavljević, Jelena, Pantelić, Ana, Kilibarda, Nataša, Vidović, Marija, "Efficient production of highly purified Late Embryogenesis Abundant (LEA) protein from Arabidopsis thaliana by recombinant DNA technology" in Biochemical Insights into Molecular Mechanisms (2021):98-99,
https://hdl.handle.net/21.15107/rcub_imagine_1870 .

TY  - CONF
AU  - Milić Komić, Sonja
AU  - Stevanović, Strahinja
AU  - Vidović, Marija
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1438
UR  - https://www.sciencedirect.com/science/article/pii/S0891584921006778
AB  - Ramonda serbica Panc. is a resurrection plant that can survive long desiccation
periods (extreme loss of cellular water). The accumulation of late embryogenesis abundant
proteins (LEAPs) is a crucial step in desiccation tolerance mechanism. Based on in vitro studies,
LEAPs can be involved in antioxidative defense, ion sequestration, structural stabilization
of both membranes and enzymes during freezing or drying, while by forming intracellular
proteinaceous condensates they increase structural integrity and intracellular viscosity of cells
during desiccation. Here we investigated the antioxidative potential of LEAPs identified by de
novo transcriptomics of R. serbica, based on their primary and secondary confirmation. In our
previous work [1], we displayed the antioxidative capacity of 20 free proteogenic amino acids
(FAA) through determining their hydroxyl radical (•
OH, generated in Fenton reaction) scavenging
rate by using electron paramagnetic resonance. These results served as a basis for generating
a model for prediction of •
OH scavenging activity for selected proteins. In addition, the model
was built based on protein primary sequences, hydrophobicity, 3D structure and predicted
solvent accessible area. Manually curated data for peptides and proteins with experimentally
determined •
OH scavenging rate were used for training and testing. The model was fed into
machine learning algorithm and •
OH scavenging potential scale was created using IC50 values.
By applying our model, we classified 164 LEAPs according to their potential for •
OH scavenging.
Further work will focus on the experimental evaluation of the obtained model by measuring of
the rate of •
OH scavenging in the presence of recombinantly produced LEAPs.
Keywords: desiccation tolerance; electron paramagnetic resonance (EPR); intrinsically disordered
proteins; machine learning; resurrection plants; secondary structure.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach
EP  - S130
IS  - Supplement 1
SP  - S129
VL  - 177
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.216
ER  - 

@conference{
author = "Milić Komić, Sonja and Stevanović, Strahinja and Vidović, Marija",
year = "2021",
abstract = "Ramonda serbica Panc. is a resurrection plant that can survive long desiccation
periods (extreme loss of cellular water). The accumulation of late embryogenesis abundant
proteins (LEAPs) is a crucial step in desiccation tolerance mechanism. Based on in vitro studies,
LEAPs can be involved in antioxidative defense, ion sequestration, structural stabilization
of both membranes and enzymes during freezing or drying, while by forming intracellular
proteinaceous condensates they increase structural integrity and intracellular viscosity of cells
during desiccation. Here we investigated the antioxidative potential of LEAPs identified by de
novo transcriptomics of R. serbica, based on their primary and secondary confirmation. In our
previous work [1], we displayed the antioxidative capacity of 20 free proteogenic amino acids
(FAA) through determining their hydroxyl radical (•
OH, generated in Fenton reaction) scavenging
rate by using electron paramagnetic resonance. These results served as a basis for generating
a model for prediction of •
OH scavenging activity for selected proteins. In addition, the model
was built based on protein primary sequences, hydrophobicity, 3D structure and predicted
solvent accessible area. Manually curated data for peptides and proteins with experimentally
determined •
OH scavenging rate were used for training and testing. The model was fed into
machine learning algorithm and •
OH scavenging potential scale was created using IC50 values.
By applying our model, we classified 164 LEAPs according to their potential for •
OH scavenging.
Further work will focus on the experimental evaluation of the obtained model by measuring of
the rate of •
OH scavenging in the presence of recombinantly produced LEAPs.
Keywords: desiccation tolerance; electron paramagnetic resonance (EPR); intrinsically disordered
proteins; machine learning; resurrection plants; secondary structure.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach",
pages = "S130-S129",
number = "Supplement 1",
volume = "177, 177",
doi = "10.1016/j.freeradbiomed.2021.08.216"
}

Milić Komić, S., Stevanović, S.,& Vidović, M.. (2021). Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177(Supplement 1), S129-S130.
https://doi.org/10.1016/j.freeradbiomed.2021.08.216

Milić Komić S, Stevanović S, Vidović M. Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach. in Free Radical Biology and Medicine. 2021;177(Supplement 1):S129-S130.
doi:10.1016/j.freeradbiomed.2021.08.216 .

Milić Komić, Sonja, Stevanović, Strahinja, Vidović, Marija, "Hydroxyl radical scavenging potential of the late embryogenesis abundant proteins (LEA) proteins from Ramonda serbica - in silico approach" in Free Radical Biology and Medicine, 177, no. Supplement 1 (2021):S129-S130,
https://doi.org/10.1016/j.freeradbiomed.2021.08.216 . .

TY  - JOUR
AU  - Milenković, Ivana
AU  - Radotić, Ksenija
AU  - Despotović, Jovana
AU  - Loncarević, Branka
AU  - Ljesević, Marija
AU  - Spasić, Slađana Z.
AU  - Nikolić, Aleksandra
AU  - Beskoski, Vladimir P.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1449
AB  - Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)
PB  - Elsevier, Amsterdam
T2  - Aquatic Toxicology
T1  - Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio
VL  - 236
DO  - 10.1016/j.aquatox.2021.105867
ER  - 

@article{
author = "Milenković, Ivana and Radotić, Ksenija and Despotović, Jovana and Loncarević, Branka and Ljesević, Marija and Spasić, Slađana Z. and Nikolić, Aleksandra and Beskoski, Vladimir P.",
year = "2021",
abstract = "Cerium oxide nanoparticles (nCeO(2)) have widespread applications, but they can be hazardous to the environment. Some reports indicate the toxic effect of nCeO(2) on tested animals, but literature data are mainly contradictory. Coating of nCeO(2) can improve their suspension stability and change their interaction with the environment, which can consequently decrease their toxic effects. Herein, the exopolysaccharides levan and pullulan, due to their high water solubility, biocompatibility, and ability to form film, were used to coat nCeO(2). Additionally, the monosaccharide glucose was used, since it is a common material for nanoparticle coating. This is the first study investigating the impact of carbohydrate-coated nCeO(2) in comparison to uncoated nCeO(2) using different model organisms. The aim of this study was to test the acute toxicity of carbohydrate-coated nCeO(2) on the bacterium Vibrio fischeri NRRL B-11177, the crustacean Daphnia magna, and zebrafish Danio rerio. The second aim was to investigate the effects of nCeO(2) on respiration in Daphnia magna which was performed for the first time. Finally, it was important to see the relation between Ce bioaccumulation in Daphnia magna and Danio rerio and other investigated parameters. Our results revealed that the coating decreased the toxicity of nCeO(2) on Vibrio fischeri. The coating of nCeO(2) did not affect the nanoparticles' accumulation/adsorption or mortality in Daphnia magna or Danio rerio. Monitoring of respiration in Daphnia magna revealed changes in CO2 production after exposure to coated nCeO(2), while the crustacean's O-2 consumption was not affected by any of the coated nCeO(2). In summary, this study revealed that, at 200 mg L-1 uncoated and carbohydrate-coated nCeO(2) are not toxic for the tested organisms, however, the CO2 production in Daphnia magna is different when they are treated with coated and uncoated nCeO(2). The highest production was in glucose and levan-coated nCeO(2) according to their highest suspension stability. Daphnia magna (D. magna), Danio rerio (D. rerio), Vibrio fischeri (V. fischeri)",
publisher = "Elsevier, Amsterdam",
journal = "Aquatic Toxicology",
title = "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio",
volume = "236",
doi = "10.1016/j.aquatox.2021.105867"
}

Milenković, I., Radotić, K., Despotović, J., Loncarević, B., Ljesević, M., Spasić, S. Z., Nikolić, A.,& Beskoski, V. P.. (2021). Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology
Elsevier, Amsterdam., 236.
https://doi.org/10.1016/j.aquatox.2021.105867

Milenković I, Radotić K, Despotović J, Loncarević B, Ljesević M, Spasić SZ, Nikolić A, Beskoski VP. Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio. in Aquatic Toxicology. 2021;236.
doi:10.1016/j.aquatox.2021.105867 .

Milenković, Ivana, Radotić, Ksenija, Despotović, Jovana, Loncarević, Branka, Ljesević, Marija, Spasić, Slađana Z., Nikolić, Aleksandra, Beskoski, Vladimir P., "Toxicity investigation of CeO2 nanoparticles coated with glucose and exopolysaccharides levan and pullulan on the bacterium Vibrio fischeri and aquatic organisms Daphnia magna and Danio rerio" in Aquatic Toxicology, 236 (2021),
https://doi.org/10.1016/j.aquatox.2021.105867 . .

TY  - DATA
AU  - Vidović, Marija
AU  - Franchin, Cinzia
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
AU  - Masi, Antonio
AU  - Arrigoni, Giorgio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1837
PB  - SpringerLink
T2  - Analytical and Bioanalytical Chemistry
T1  - Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1837
ER  - 

@misc{
author = "Vidović, Marija and Franchin, Cinzia and Morina, Filis and Veljović-Jovanović, Sonja and Masi, Antonio and Arrigoni, Giorgio",
year = "2020",
publisher = "SpringerLink",
journal = "Analytical and Bioanalytical Chemistry",
title = "Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1837"
}

Vidović, M., Franchin, C., Morina, F., Veljović-Jovanović, S., Masi, A.,& Arrigoni, G.. (2020). Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.. in Analytical and Bioanalytical Chemistry
SpringerLink..
https://hdl.handle.net/21.15107/rcub_imagine_1837

Vidović M, Franchin C, Morina F, Veljović-Jovanović S, Masi A, Arrigoni G. Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2.. in Analytical and Bioanalytical Chemistry. 2020;.
https://hdl.handle.net/21.15107/rcub_imagine_1837 .

Vidović, Marija, Franchin, Cinzia, Morina, Filis, Veljović-Jovanović, Sonja, Masi, Antonio, Arrigoni, Giorgio, "Supplementary data for the article: Vidović, M.; Franchin, C.; Morina, F.; Veljović-Jovanović, S.; Masi, A.; Arrigoni, G. Efficient Protein Extraction for Shotgun Proteomics from Hydrated and Desiccated Leaves of Resurrection Ramonda Serbica Plants. Anal Bioanal Chem 2020, 412 (30), 8299–8312. https://doi.org/10.1007/s00216-020-02965-2." in Analytical and Bioanalytical Chemistry (2020),
https://hdl.handle.net/21.15107/rcub_imagine_1837 .

TY  - JOUR
AU  - Vidović, Marija
AU  - Franchin, Cinzia
AU  - Morina, Filis
AU  - Veljović-Jovanović, Sonja
AU  - Masi, Antonio
AU  - Arrigoni, Giorgio
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1364
AB  - Resurrection plantRamonda serbicais a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high amount of phenolics and polysaccharide, and possible protein modifications and aggregations during the extraction and purification steps. When applied toR.serbicaleaves, widely used protein extraction protocols containing polyvinylpolypyrrolidone and ascorbate, as well as the phenol/SDS/buffer-based protocol recommended for recalcitrant plant tissues failed to eliminate persistent contamination and ensure high protein quality. Here we compared three protein extraction approaches aiming to establish the optimal one for both hydrated and desiccatedR.serbicaleaves. To evaluate the efficacy of these protocols by shotgun proteomics, we also created the firstR.serbicaannotated transcriptome database, available at. The detergent-free phenol-based extraction combined with dodecyl-beta-D-maltoside-assisted extraction enabled high-yield and high-purity protein extracts. The phenol-based protocol improved the protein-band resolution, band number, and intensity upon electrophoresis, and increased the protein yield and the number of identified peptides and protein groups by LC-MS/MS. Additionally, dodecyl-beta-D-maltoside enabled solubilisation and identification of more membrane-associated proteins. The presented study paves the way for investigating the desiccation tolerance inR.serbica, and we recommend this protocol for similar recalcitrant plant material.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants
EP  - 8312
IS  - 30
SP  - 8299
VL  - 412
DO  - 10.1007/s00216-020-02965-2
ER  - 

@article{
author = "Vidović, Marija and Franchin, Cinzia and Morina, Filis and Veljović-Jovanović, Sonja and Masi, Antonio and Arrigoni, Giorgio",
year = "2020",
abstract = "Resurrection plantRamonda serbicais a suitable model to investigate vegetative desiccation tolerance. However, the detailed study of these mechanisms at the protein level is hampered by the severe tissue water loss, high amount of phenolics and polysaccharide, and possible protein modifications and aggregations during the extraction and purification steps. When applied toR.serbicaleaves, widely used protein extraction protocols containing polyvinylpolypyrrolidone and ascorbate, as well as the phenol/SDS/buffer-based protocol recommended for recalcitrant plant tissues failed to eliminate persistent contamination and ensure high protein quality. Here we compared three protein extraction approaches aiming to establish the optimal one for both hydrated and desiccatedR.serbicaleaves. To evaluate the efficacy of these protocols by shotgun proteomics, we also created the firstR.serbicaannotated transcriptome database, available at. The detergent-free phenol-based extraction combined with dodecyl-beta-D-maltoside-assisted extraction enabled high-yield and high-purity protein extracts. The phenol-based protocol improved the protein-band resolution, band number, and intensity upon electrophoresis, and increased the protein yield and the number of identified peptides and protein groups by LC-MS/MS. Additionally, dodecyl-beta-D-maltoside enabled solubilisation and identification of more membrane-associated proteins. The presented study paves the way for investigating the desiccation tolerance inR.serbica, and we recommend this protocol for similar recalcitrant plant material.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants",
pages = "8312-8299",
number = "30",
volume = "412",
doi = "10.1007/s00216-020-02965-2"
}

Vidović, M., Franchin, C., Morina, F., Veljović-Jovanović, S., Masi, A.,& Arrigoni, G.. (2020). Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 412(30), 8299-8312.
https://doi.org/10.1007/s00216-020-02965-2

Vidović M, Franchin C, Morina F, Veljović-Jovanović S, Masi A, Arrigoni G. Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants. in Analytical and Bioanalytical Chemistry. 2020;412(30):8299-8312.
doi:10.1007/s00216-020-02965-2 .

Vidović, Marija, Franchin, Cinzia, Morina, Filis, Veljović-Jovanović, Sonja, Masi, Antonio, Arrigoni, Giorgio, "Efficient protein extraction for shotgun proteomics from hydrated and desiccated leaves of resurrection Ramonda serbica plants" in Analytical and Bioanalytical Chemistry, 412, no. 30 (2020):8299-8312,
https://doi.org/10.1007/s00216-020-02965-2 . .

TY  - JOUR
AU  - Zivanović, Bojana
AU  - Komić, Sonja Milic
AU  - Tosti, Tomislav
AU  - Vidović, Marija
AU  - Prokić, Ljiljana
AU  - Veljović-Jovanović, Sonja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1342
AB  - Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.
PB  - MDPI : Basel
T2  - Plants
T1  - Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato
IS  - 9
SP  - 1147
VL  - 9
DO  - 10.3390/plants9091147
ER  - 

@article{
author = "Zivanović, Bojana and Komić, Sonja Milic and Tosti, Tomislav and Vidović, Marija and Prokić, Ljiljana and Veljović-Jovanović, Sonja",
year = "2020",
abstract = "Water deficit has a global impact on plant growth and crop yield. Climate changes are going to increase the intensity, duration and frequency of severe droughts, particularly in southern and south-eastern Europe, elevating the water scarcity issues. We aimed to assess the contribution of endogenous abscisic acid (ABA) in the protective mechanisms against water deficit, including stomatal conductance, relative water potential and the accumulation of osmoprotectants, as well as on growth parameters. To achieve that, we used a suitable model system, ABA-deficient tomato mutant, flacca and its parental line. Flacca mutant exhibited constitutively higher levels of soluble sugars (e.g., galactose, arabinose, sorbitol) and free amino acids (AAs) compared with the wild type (WT). Water deficit provoked the strong accumulation of proline in both genotypes, and total soluble sugars only in flacca. Upon re-watering, these osmolytes returned to the initial levels in both genotypes. Our results indicate that flacca compensated higher stomatal conductance with a higher constitutive level of free sugars and AAs. Additionally, we suggest that the accumulation of AAs, particularly proline and its precursors and specific branched-chain AAs in both, glucose and sucrose in flacca, and sorbitol in WT, could contribute to maintaining growth rate during water deficit and recovery in both tomato genotypes.",
publisher = "MDPI : Basel",
journal = "Plants",
title = "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato",
number = "9",
pages = "1147",
volume = "9",
doi = "10.3390/plants9091147"
}

Zivanović, B., Komić, S. M., Tosti, T., Vidović, M., Prokić, L.,& Veljović-Jovanović, S.. (2020). Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants
MDPI : Basel., 9(9), 1147.
https://doi.org/10.3390/plants9091147

Zivanović B, Komić SM, Tosti T, Vidović M, Prokić L, Veljović-Jovanović S. Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato. in Plants. 2020;9(9):1147.
doi:10.3390/plants9091147 .

Zivanović, Bojana, Komić, Sonja Milic, Tosti, Tomislav, Vidović, Marija, Prokić, Ljiljana, Veljović-Jovanović, Sonja, "Leaf Soluble Sugars and Free Amino Acids as Important Components of Abscisic Acid-Mediated Drought Response in Tomato" in Plants, 9, no. 9 (2020):1147,
https://doi.org/10.3390/plants9091147 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Cuković, Katarina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1332
AB  - Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additional challenge in total RNA extraction. Here, we evaluated and compared several already established TRIzol- and CTAB-based protocols aiming to develop the efficient, simple and low-cost methods for the extraction of the satisfactory yield RNA of great purity and integrity, required for the construction of high-quality cDNA libraries. Our results show that the CTAB-based protocol (i.e. CTAB 1b) enabled the extraction of high-quality RNA from photosynthetically active and non-photosynthetically active leaf sectors of P. zonale, with high RIN values. On the other hand, TRIzol-based protocol provided a high RNA yield with low contamination and high RNA integrity even in desiccated leaves of R. serbica. We envisage that the proposed protocol would be suitable for the RNA extractions from other desiccated organs (e.g. seeds, grains, pollen grains).
PB  - Heidelberg : Springer Heidelberg
T2  - 3 Biotech
T1  - Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants
IS  - 6
VL  - 10
DO  - 10.1007/s13205-020-02279-1
ER  - 

@article{
author = "Vidović, Marija and Cuković, Katarina",
year = "2020",
abstract = "Resurrection plant Ramonda serbica is a suitable model to investigate mechanisms of desiccation tolerance, while variegated Pelargonium zonale has been proven to serve as an excellent model for the metabolite allocation between sink tissue and source tissue within the same organ. However, the genomes of these plants are still not sequenced, limiting their application in molecular studies. To investigate the transcript abundance by next-generation sequencing, high-quality RNA input is required. Leaves of both P. zonale and R. serbica are rich in polyphenols that interfere with high-quality RNA extraction by common protocols. Moreover, low water content and high amount of sugars and other osmoprotectants in desiccated R. serbica leaves present the additional challenge in total RNA extraction. Here, we evaluated and compared several already established TRIzol- and CTAB-based protocols aiming to develop the efficient, simple and low-cost methods for the extraction of the satisfactory yield RNA of great purity and integrity, required for the construction of high-quality cDNA libraries. Our results show that the CTAB-based protocol (i.e. CTAB 1b) enabled the extraction of high-quality RNA from photosynthetically active and non-photosynthetically active leaf sectors of P. zonale, with high RIN values. On the other hand, TRIzol-based protocol provided a high RNA yield with low contamination and high RNA integrity even in desiccated leaves of R. serbica. We envisage that the proposed protocol would be suitable for the RNA extractions from other desiccated organs (e.g. seeds, grains, pollen grains).",
publisher = "Heidelberg : Springer Heidelberg",
journal = "3 Biotech",
title = "Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants",
number = "6",
volume = "10",
doi = "10.1007/s13205-020-02279-1"
}

Vidović, M.,& Cuković, K.. (2020). Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants. in 3 Biotech
Heidelberg : Springer Heidelberg., 10(6).
https://doi.org/10.1007/s13205-020-02279-1

Vidović M, Cuković K. Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants. in 3 Biotech. 2020;10(6).
doi:10.1007/s13205-020-02279-1 .

Vidović, Marija, Cuković, Katarina, "Isolation of high-quality RNA from recalcitrant leaves of variegated and resurrection plants" in 3 Biotech, 10, no. 6 (2020),
https://doi.org/10.1007/s13205-020-02279-1 . .

TY  - JOUR
AU  - Hajiboland, Roghieh
AU  - Sadeghzadeh, Noushin
AU  - Bosnić, Dragana
AU  - Bosnić, Predrag
AU  - Tolra, Roser
AU  - Poschenrieder, Charlotte
AU  - Nikolić, Miroslav
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1331
AB  - Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.
PB  - Springer, Dordrecht
T2  - Plant and Soil
T1  - Selenium activates components of iron acquisition machinery in oilseed rape roots
EP  - 586
IS  - 1-2
SP  - 569
VL  - 452
DO  - 10.1007/s11104-020-04599-w
ER  - 

@article{
author = "Hajiboland, Roghieh and Sadeghzadeh, Noushin and Bosnić, Dragana and Bosnić, Predrag and Tolra, Roser and Poschenrieder, Charlotte and Nikolić, Miroslav",
year = "2020",
abstract = "Aims Beneficial elements may stimulate plant growth by favoring the availability of essential nutrients for metabolic processes. This study addresses the still unexplored question whether the beneficial action of selenium (Se) on plants can be due to its interaction with iron (Fe). Methods Oilseed rape (Brassica napus) plants were grown hydroponically without (-Se) or with 10 mu M Na2SeO4(+Se) and exposed to either Fe adequate (+Fe) or starvation (-Fe) treatments. Results Selenium enhanced leaf chlorophyll concentration, photochemical parameters and CO(2)fixation. Although Se did not significantly increase total leaf Fe concentration, the concentration of cytoplasmic Fe(II)pool was enhanced under -Fe + Se conditions. However, the most conspicuous Se effects occurred in Fe-deficient roots. Selenium upregulated both the root expression levels of genes encodingFERRIC-REDUCTION OXIDASE (FRO1)and the activity of FRO and exacerbated the stimulatory effect of -Fe on the exudation rate of organic acids and phenolic compounds. Both -Se and + Se plants used Fe(III)EDTA in similar way, while only +Se plants were able to take advantage of the sparingly soluble Fe(OH)(3). In order to explore the mechanism of Se action, we recorded higher endogenous level of NO and upregulation ofETHYLENE RESPONSE FACTOR 2(ERF2) by Se irrespective of Fe conditions. Conclusions Our results show that Se augments root machinery for Fe acquisition mainly through activation of Fe signaling components.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Selenium activates components of iron acquisition machinery in oilseed rape roots",
pages = "586-569",
number = "1-2",
volume = "452",
doi = "10.1007/s11104-020-04599-w"
}

Hajiboland, R., Sadeghzadeh, N., Bosnić, D., Bosnić, P., Tolra, R., Poschenrieder, C.,& Nikolić, M.. (2020). Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil
Springer, Dordrecht., 452(1-2), 569-586.
https://doi.org/10.1007/s11104-020-04599-w

Hajiboland R, Sadeghzadeh N, Bosnić D, Bosnić P, Tolra R, Poschenrieder C, Nikolić M. Selenium activates components of iron acquisition machinery in oilseed rape roots. in Plant and Soil. 2020;452(1-2):569-586.
doi:10.1007/s11104-020-04599-w .

Hajiboland, Roghieh, Sadeghzadeh, Noushin, Bosnić, Dragana, Bosnić, Predrag, Tolra, Roser, Poschenrieder, Charlotte, Nikolić, Miroslav, "Selenium activates components of iron acquisition machinery in oilseed rape roots" in Plant and Soil, 452, no. 1-2 (2020):569-586,
https://doi.org/10.1007/s11104-020-04599-w . .