TY  - CONF
AU  - Bisenić, Aleksandar
AU  - Tomić, Sergej
AU  - Bekić, Marina
AU  - Pavlović, Luka
AU  - Dinić, Miroslav
AU  - Terzić- Vidojević, Amarela
AU  - Radojević, Dušan
AU  - Soković Bajić, Svetlana
AU  - Mitrović, Hristina
AU  - Jakovljević, Stefan
AU  - Stevanović, Dušan
AU  - Golić, Nataša
AU  - Đokić, Jelena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2374
AB  - Alterations in gut microbiota and deregulation
of the gut immune system are recognized
as important events in autoimmune diseases.
The knowledge about the important role of anaerobic
gut bacteria that produce short-chain
fatty acids (SCFAs), in the regulation of intestinal
barrier and immune response made a way
for the development of microbiota-based interventions.
Our research aimed to isolate the
strains with the potential to produce SCFAs,
from healthy volunteer fecal material, and to
test their effects on IL-8 production in the culture
of intestinal epithelial cells (Caco2) as an in
vitro system imitating initial intestinal inflammation,
the effects on the expression of neuronal
activity-regulated genes of Caenorhabditis
elegans, and the effect on the development
of experimental autoimmune encephalomyelitis
(EAE), a mouse model of multiple  sclerosis.
Three isolated butyric acid (BA)-producing
strains, and three acetic acid (AA)-producing
strains diminished the production of IL-8 in Caco-
2 cells treated with IL-1β/TNF-α. Further, all
BA-producing strains stimulated the expression
of important neuro-related genes in C. elegans.
Based on the strongest effects in these
assays an isolate identified as Faecalimonas sp.
NGB245 strain was further tested in EAE model.
The oral treatment of EAE-induced mice with
this strain for 16h per day for 15 days resulted
in alleviated daily clinical scores, maximal
clinical scores, and the duration of the illness
in comparison to the effect of media used for
strain cultivation. These results point to the potential
of NGB245 to modify the gut-brain axis
opening the field for future development of microbiota-
based therapy for the diseases associated
with immune response dysfunctions.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS
EP  - 116
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2374
ER  - 

@conference{
author = "Bisenić, Aleksandar and Tomić, Sergej and Bekić, Marina and Pavlović, Luka and Dinić, Miroslav and Terzić- Vidojević, Amarela and Radojević, Dušan and Soković Bajić, Svetlana and Mitrović, Hristina and Jakovljević, Stefan and Stevanović, Dušan and Golić, Nataša and Đokić, Jelena",
year = "2024",
abstract = "Alterations in gut microbiota and deregulation
of the gut immune system are recognized
as important events in autoimmune diseases.
The knowledge about the important role of anaerobic
gut bacteria that produce short-chain
fatty acids (SCFAs), in the regulation of intestinal
barrier and immune response made a way
for the development of microbiota-based interventions.
Our research aimed to isolate the
strains with the potential to produce SCFAs,
from healthy volunteer fecal material, and to
test their effects on IL-8 production in the culture
of intestinal epithelial cells (Caco2) as an in
vitro system imitating initial intestinal inflammation,
the effects on the expression of neuronal
activity-regulated genes of Caenorhabditis
elegans, and the effect on the development
of experimental autoimmune encephalomyelitis
(EAE), a mouse model of multiple  sclerosis.
Three isolated butyric acid (BA)-producing
strains, and three acetic acid (AA)-producing
strains diminished the production of IL-8 in Caco-
2 cells treated with IL-1β/TNF-α. Further, all
BA-producing strains stimulated the expression
of important neuro-related genes in C. elegans.
Based on the strongest effects in these
assays an isolate identified as Faecalimonas sp.
NGB245 strain was further tested in EAE model.
The oral treatment of EAE-induced mice with
this strain for 16h per day for 15 days resulted
in alleviated daily clinical scores, maximal
clinical scores, and the duration of the illness
in comparison to the effect of media used for
strain cultivation. These results point to the potential
of NGB245 to modify the gut-brain axis
opening the field for future development of microbiota-
based therapy for the diseases associated
with immune response dysfunctions.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS",
pages = "116-116",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2374"
}

Bisenić, A., Tomić, S., Bekić, M., Pavlović, L., Dinić, M., Terzić- Vidojević, A., Radojević, D., Soković Bajić, S., Mitrović, H., Jakovljević, S., Stevanović, D., Golić, N.,& Đokić, J.. (2024). SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2374

Bisenić A, Tomić S, Bekić M, Pavlović L, Dinić M, Terzić- Vidojević A, Radojević D, Soković Bajić S, Mitrović H, Jakovljević S, Stevanović D, Golić N, Đokić J. SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2374 .

Bisenić, Aleksandar, Tomić, Sergej, Bekić, Marina, Pavlović, Luka, Dinić, Miroslav, Terzić- Vidojević, Amarela, Radojević, Dušan, Soković Bajić, Svetlana, Mitrović, Hristina, Jakovljević, Stefan, Stevanović, Dušan, Golić, Nataša, Đokić, Jelena, "SHORT-CHAIN FATTY ACID-PRODUCING FAECALIMONAS SP. NGB245 STRAIN REGULATES THE EXPRESSION OF NEURONAL ACTIVITY-REGULATED GENES AND ATTENUATES THE SYMPTOMS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):116-116,
https://hdl.handle.net/21.15107/rcub_imagine_2374 .

TY  - CONF
AU  - Dinić, Miroslav
AU  - L. Burgess, Jamie
AU  - Lukić, Jovanka
AU  - Catanuto, Paola
AU  - Radojević, Dušan
AU  - Marjanović, Jelena
AU  - Verpile, Rebecca
AU  - R. Thaller, Seth
AU  - Gonzalez, Tammy
AU  - Golić, Nataša
AU  - Tomić- Canić, Marjana
AU  - Strahinić, Ivana
AU  - Pastar, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2378
AB  - Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING
EP  - 133
SP  - 133
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2378
ER  - 

@conference{
author = "Dinić, Miroslav and L. Burgess, Jamie and Lukić, Jovanka and Catanuto, Paola and Radojević, Dušan and Marjanović, Jelena and Verpile, Rebecca and R. Thaller, Seth and Gonzalez, Tammy and Golić, Nataša and Tomić- Canić, Marjana and Strahinić, Ivana and Pastar, Irena",
year = "2024",
abstract = "Skin microbiome emerged as an important
factor which can balance tissue repair process
and wound healing. Recent evidence suggest
that intracellular bacterial localization could be
associated with the aberrant healing observed
in patients with chronic wounds, while therapeutics
targeting intracellular bacteria remain
limited. Probiotic lactobacilli and their bioactive
lysates (postbiotics) are well known for their role
in maintenance of gut epithelial homeostasis.
Hence, in this study we focused to understand
the mechanisms of cutaneous response to fourteen
postbiotics derived from different lactobacilli
to reduce intracellular Staphylococcus aureus
colonization and promote healing. Latilactobacillus
curvatus BGMK2-41 demonstrated the
most efficient capability to reduce intracellular infection by S. aureus in keratinocytes in vitro and
infection of human skin explants. Reduction of
bacterial number was followed by upregulation
of the expression of antimicrobial response
genes. Furthermore, BGMK2-41 postbiotic treatment
stimulates keratinocyte migration in vitro
and increases expression of anti-inflammatory
cytokine IL-10, promotes wound closure and
strengthens the epidermal barrier via upregulation
of tight junction proteins in a human ex vivo
wound model. Altogether, this study provided
evidence that postbiotics could stimulate fortification
of epithelial barrier to suppress dissemination
of intracellular pathogens which can be
used as a novel approach to treat dermatologic
and wound healing disorders associated with
persistent infections.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING",
pages = "133-133",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2378"
}

Dinić, M., L. Burgess, J., Lukić, J., Catanuto, P., Radojević, D., Marjanović, J., Verpile, R., R. Thaller, S., Gonzalez, T., Golić, N., Tomić- Canić, M., Strahinić, I.,& Pastar, I.. (2024). HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378

Dinić M, L. Burgess J, Lukić J, Catanuto P, Radojević D, Marjanović J, Verpile R, R. Thaller S, Gonzalez T, Golić N, Tomić- Canić M, Strahinić I, Pastar I. HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:133-133.
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

Dinić, Miroslav, L. Burgess, Jamie, Lukić, Jovanka, Catanuto, Paola, Radojević, Dušan, Marjanović, Jelena, Verpile, Rebecca, R. Thaller, Seth, Gonzalez, Tammy, Golić, Nataša, Tomić- Canić, Marjana, Strahinić, Ivana, Pastar, Irena, "HOST-MICROBIOTA INTERPLAY REGULATES EPITHELIAL BARRIER FUNCTION AND WOUND HEALING" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):133-133,
https://hdl.handle.net/21.15107/rcub_imagine_2378 .

TY  - CONF
AU  - Golić, Nataša
AU  - Terzić Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Dinić, Miroslav
AU  - Đokić, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Lukić, Jovanka
AU  - Živković, Milica
AU  - Nastasijević, Branislav
AU  - Soković, Svetlana
AU  - Brdarić, Emilija
AU  - Radojević, Dušan
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2372
AB  - There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faecalimonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS
EP  - 106
SP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2372
ER  - 

@conference{
author = "Golić, Nataša and Terzić Vidojević, Amarela and Tolinački, Maja and Dinić, Miroslav and Đokić, Jelena and Todorović Vukotić, Nevena and Lukić, Jovanka and Živković, Milica and Nastasijević, Branislav and Soković, Svetlana and Brdarić, Emilija and Radojević, Dušan",
year = "2024",
abstract = "There has been an epidemic of various non-communicable
degenerative and autoimmune diseases,
strongly associated with the modern
lifestyle. Among them, neurodegenerative and
psychiatric disorders represent a huge burden on
society. Recently, all these diseases have been associated
with the gut microbiota dysbiosis. Gut
microbiota-host interaction research has been
greatly improved due to development of molecular
high-throughput techniques based on
various ‘omics’ techniques coupled with bioinformatics
and data science developments. However,
the mechanisms of the host–microbiota crosstalk
are still poorly understood. The NextGenBiotics
project proposes an innovative integrative
multi-omics research strategy for deciphering
the mechanism behind the cross-talk among
microbiota and gut-brain-axis. The 118 novel
NGPs candidates belonging to Dorea sp., Blautia
sp., Bacteroides sp., Roseburia sp., Sellimonas
sp., Faecalicatena sp., Phascolarctobacterium faecium,
and Faecalimonas sp. were cultivated. The
25 NGPs with confirmed safe status and potential
probiotic potential were screened in C. elegans
model for their effects on behavioural and neuronal
activity. The most prominent candidates
with ability to upregulate expression of genes
involved in neurotransmiting are further tested
in EAE (an animal model for MS) and CUMS depression
model. The specific microbiota-derived
metabolites have been identified as potential
neuro- and psycho-biotics. The NextGenBiotics is
highly ambitious project, dedicated to pioneering
work in the field of multi-omics studies related
to the cultivation of novel anaerobic NGPs
and the studying of their effect on MGBA. This
concept enabled studying bidirectional communication
between gut microbiota and brain
on the functional level that will significantly
contribute to the growing body data related to
MGBA. The results obtained during NextGenBiotics
determined the genes/metabolites and the
associated mechanisms involved in health-promoting
effects of NGPs in MGBA beyond stateof-
the-art, broadening the scientific knowledge
and opening up the possible novel therapeutic
approaches in prevention and therapy of neurodegenerative
and psychiatric diseases.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS",
pages = "106-106",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2372"
}

Golić, N., Terzić Vidojević, A., Tolinački, M., Dinić, M., Đokić, J., Todorović Vukotić, N., Lukić, J., Živković, M., Nastasijević, B., Soković, S., Brdarić, E.,& Radojević, D.. (2024). THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 106-106.
https://hdl.handle.net/21.15107/rcub_imagine_2372

Golić N, Terzić Vidojević A, Tolinački M, Dinić M, Đokić J, Todorović Vukotić N, Lukić J, Živković M, Nastasijević B, Soković S, Brdarić E, Radojević D. THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:106-106.
https://hdl.handle.net/21.15107/rcub_imagine_2372 .

Golić, Nataša, Terzić Vidojević, Amarela, Tolinački, Maja, Dinić, Miroslav, Đokić, Jelena, Todorović Vukotić, Nevena, Lukić, Jovanka, Živković, Milica, Nastasijević, Branislav, Soković, Svetlana, Brdarić, Emilija, Radojević, Dušan, "THE USE OF INTEGRATIVE MULTI-OMICS APPROACH IN CULTIVATION AND CHARACTERIZATION OF GUT BACTERIA RELATED TO MICROBIOTA-GUT-BRAIN AXIS AS A SOURCE FOR NEXT GENERATION PROBIOTICS" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):106-106,
https://hdl.handle.net/21.15107/rcub_imagine_2372 .

TY  - CONF
AU  - Ćurčić, Jovana
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Stanisavljević, Nemanja
AU  - Jovčić, Branko
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2380
AB  - Pseudomonas aeruginosa is a Gram-negative
pathogen responsible for frequent hospital-acquired
infections of the bloodstream, the respiratory
tract, and the urinary tract. Quorum
quenching enzymes are recognized as an alternative
antivirulence approach targeting pathogenic
bacteria. The efficacy of YtnP lactonase in
reducing the virulence of P. aeruginosa MMA83
in vivo using Caenorhabditis elegans as a model
system was investigated. The recombinant YtnP
lactonase exhibits no cytotoxicity, demonstrated
by its lack of harmful effects on both the
immortalized human HaCaT cell line and two
strains of C. elegans (AU37 and N2 wild-type). In
a toxin-mediated killing liquid assay, the survival
rates of C. elegans AU37 mutant and N2 wildtype
strains infected with the clinical isolate P.
aeruginosa MMA83 significantly increased when
pre-treated with YtnP lactonase, compared to
untreated controls. Considering that virulence
factors expression is regulated by quorum sensing
(QS) signaling it is hypothesized that YtnP
lactonase prolongs the life span of C. elegans
by downregulating the QS and expression of
virulence factors of MMA83. The protective effects
of YtnP lactonase against MMA83-induced
pathogenicity in C. elegans, coupled with its absence
of cytotoxicity, position YtnP lactonase as
a promising prophylactic agent with antivirulence
properties.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION
EP  - 143
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2380
ER  - 

@conference{
author = "Ćurčić, Jovana and Malešević, Milka and Dinić, Miroslav and Novović, Katarina and Vasiljević, Zorica and Stanisavljević, Nemanja and Jovčić, Branko",
year = "2024",
abstract = "Pseudomonas aeruginosa is a Gram-negative
pathogen responsible for frequent hospital-acquired
infections of the bloodstream, the respiratory
tract, and the urinary tract. Quorum
quenching enzymes are recognized as an alternative
antivirulence approach targeting pathogenic
bacteria. The efficacy of YtnP lactonase in
reducing the virulence of P. aeruginosa MMA83
in vivo using Caenorhabditis elegans as a model
system was investigated. The recombinant YtnP
lactonase exhibits no cytotoxicity, demonstrated
by its lack of harmful effects on both the
immortalized human HaCaT cell line and two
strains of C. elegans (AU37 and N2 wild-type). In
a toxin-mediated killing liquid assay, the survival
rates of C. elegans AU37 mutant and N2 wildtype
strains infected with the clinical isolate P.
aeruginosa MMA83 significantly increased when
pre-treated with YtnP lactonase, compared to
untreated controls. Considering that virulence
factors expression is regulated by quorum sensing
(QS) signaling it is hypothesized that YtnP
lactonase prolongs the life span of C. elegans
by downregulating the QS and expression of
virulence factors of MMA83. The protective effects
of YtnP lactonase against MMA83-induced
pathogenicity in C. elegans, coupled with its absence
of cytotoxicity, position YtnP lactonase as
a promising prophylactic agent with antivirulence
properties.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2380"
}

Ćurčić, J., Malešević, M., Dinić, M., Novović, K., Vasiljević, Z., Stanisavljević, N.,& Jovčić, B.. (2024). YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2380

Ćurčić J, Malešević M, Dinić M, Novović K, Vasiljević Z, Stanisavljević N, Jovčić B. YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2380 .

Ćurčić, Jovana, Malešević, Milka, Dinić, Miroslav, Novović, Katarina, Vasiljević, Zorica, Stanisavljević, Nemanja, Jovčić, Branko, "YTNP LACTONASE IMPROVES THE ABILITY OF CAENORHABDITIS ELEGANS TO SURVIVE PSEUDOMONAS AERUGINOSA MMA83 INFECTION" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):143-143,
https://hdl.handle.net/21.15107/rcub_imagine_2380 .

TY  - JOUR
AU  - Ćurčić, Jovana
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Malešević, Milka
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0141813024012248
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2326
AB  - Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T2  - International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules
T1  - A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo
SP  - 130421
DO  - 10.1016/j.ijbiomac.2024.130421
ER  - 

@article{
author = "Ćurčić, Jovana and Dinić, Miroslav and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Malešević, Milka",
year = "2024",
abstract = "Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules, International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules",
title = "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo",
pages = "130421",
doi = "10.1016/j.ijbiomac.2024.130421"
}

Ćurčić, J., Dinić, M., Novović, K., Vasiljević, Z., Kojić, M., Jovčić, B.,& Malešević, M.. (2024). A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules
Elsevier., 130421.
https://doi.org/10.1016/j.ijbiomac.2024.130421

Ćurčić J, Dinić M, Novović K, Vasiljević Z, Kojić M, Jovčić B, Malešević M. A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules. 2024;:130421.
doi:10.1016/j.ijbiomac.2024.130421 .

Ćurčić, Jovana, Dinić, Miroslav, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Malešević, Milka, "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo" in International Journal of Biological Macromolecules (2024):130421,
https://doi.org/10.1016/j.ijbiomac.2024.130421 . .

TY  - CONF
AU  - Mitrović, Hristina
AU  - Brdarić, Emilija
AU  - Bisenić, Aleksandar
AU  - Jakovljević, Stefan
AU  - Dinić, Miroslav
AU  - Đokić, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Tolinački, Maja
AU  - Radojević, Dušan
AU  - Golić, Nataša
AU  - Soković Bajić, Svetlana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2389
AB  - Psychobiotics are live bacterial strains impacting the central nervous system, producing
neuroactive substances like GABA. GABA from
microbiota influences neural signals, affecting
neurological parameters, sleep, appetite, mood,
and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and
the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary
glutamate, with Lactobacillus rhamnosus shown
to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge
exists about anaerobic GABA producers, warranting further research for a comprehensive
understanding. Material for isolation comprised
fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within
a maximum of 1 hour after sampling. Isolated
bacteria were identified through sequencing
the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium
contains hematine and vitamin K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a
role in oxygen reduction, and 0.5% glutamate, a
precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial
species was determined using the TLC method.
Quantification of GABA was performed using the
HPLC method. Furthermore, the positive effects
observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential
anti-inflammatory effects of these postbiotics.
The study implies anti-inflammatory effects of
anaerobic GABA producers, offering insights into the complex interplay among gut microbiota,
immune function, and mental health. Recognizing inflammation’s role in depressive symptoms,
targeting anaerobic bacteria involved in GABA
synthesis could modulate neurotransmitters and
inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic
understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This
offers avenues for novel therapeutic approaches
and enhances overall quality of life.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA
EP  - 111
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2389
ER  - 

@conference{
author = "Mitrović, Hristina and Brdarić, Emilija and Bisenić, Aleksandar and Jakovljević, Stefan and Dinić, Miroslav and Đokić, Jelena and Terzić-Vidojević, Amarela and Tolinački, Maja and Radojević, Dušan and Golić, Nataša and Soković Bajić, Svetlana",
year = "2024",
abstract = "Psychobiotics are live bacterial strains impacting the central nervous system, producing
neuroactive substances like GABA. GABA from
microbiota influences neural signals, affecting
neurological parameters, sleep, appetite, mood,
and cognition, traversing the intestinal barrier to bind to receptors on enteric neurons and
the vagus nerve. Lactobacillus and Bifidobacterium species can synthesize GABA from dietary
glutamate, with Lactobacillus rhamnosus shown
to reduce anxiety and depressive behavior, elevating hippocampal GABA. Limited knowledge
exists about anaerobic GABA producers, warranting further research for a comprehensive
understanding. Material for isolation comprised
fecal samples from healthy donors, with isolation conducted in an anaerobic chamber within
a maximum of 1 hour after sampling. Isolated
bacteria were identified through sequencing
the 16S rRNA gene. For bacterial cultivation, different types of media were used. PYG medium
contains hematine and vitamin K, essential supplements for the cultivation of anaerobic bacteria. All media included 0.1% L-cysteine, playing a
role in oxygen reduction, and 0.5% glutamate, a
precursor for GABA production. After identification, the presence of GABA in 8 tested bacterial
species was determined using the TLC method.
Quantification of GABA was performed using the
HPLC method. Furthermore, the positive effects
observed in Caco2 cells with induced inflammation, after treatment with certain anaerobic postbiotics producing GABA, indicate the potential
anti-inflammatory effects of these postbiotics.
The study implies anti-inflammatory effects of
anaerobic GABA producers, offering insights into the complex interplay among gut microbiota,
immune function, and mental health. Recognizing inflammation’s role in depressive symptoms,
targeting anaerobic bacteria involved in GABA
synthesis could modulate neurotransmitters and
inflammatory responses, presenting a comprehensive approach to mental well-being. Advancing research in this area contributes to a holistic
understanding of anaerobic bacteria, GABA production, gut microbiota, and mental health. This
offers avenues for novel therapeutic approaches
and enhances overall quality of life.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA",
pages = "111-111",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2389"
}

Mitrović, H., Brdarić, E., Bisenić, A., Jakovljević, S., Dinić, M., Đokić, J., Terzić-Vidojević, A., Tolinački, M., Radojević, D., Golić, N.,& Soković Bajić, S.. (2024). FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 111-111.
https://hdl.handle.net/21.15107/rcub_imagine_2389

Mitrović H, Brdarić E, Bisenić A, Jakovljević S, Dinić M, Đokić J, Terzić-Vidojević A, Tolinački M, Radojević D, Golić N, Soković Bajić S. FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:111-111.
https://hdl.handle.net/21.15107/rcub_imagine_2389 .

Mitrović, Hristina, Brdarić, Emilija, Bisenić, Aleksandar, Jakovljević, Stefan, Dinić, Miroslav, Đokić, Jelena, Terzić-Vidojević, Amarela, Tolinački, Maja, Radojević, Dušan, Golić, Nataša, Soković Bajić, Svetlana, "FROM GUT TO LAB: UNLOCKING ANTI-INFLAMMATORY POTENTIAL WITH GABA-PRODUCING BACTERIA" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):111-111,
https://hdl.handle.net/21.15107/rcub_imagine_2389 .

TY  - CONF
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Radojević, Dušan
AU  - Brdarić, Emilija
AU  - Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Golić, Nataša
PY  - 2023
UR  - https://www.microbiota-site.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2189
AB  - Introduction: New theories of ageing recognized gut microbiota as one of twelve hallmarks of ageing (1). 
Recent data conducted on Caenorhabditis elegans imply a potential role of Lactobacillus species and other 
commensal bacteria 
in regulation of ageing highlighting TFEB/HLH-30-dependent autophagy, p38 
MAPK/PMK-1 signalling and mitochondrial function as activated longevity-associated mechanisms (2,3). 
Here, we explore the potential of bacterial polysaccharides 
loosely attached to bacterial cell wall 
(exopolysaccharides), considering it is still unknown which bacterial molecules could activate longevity 
signalling pathways.
Materials & Methods: Caenorhabditis elegans was used as ageing model. Evaluation of worm’s lifespan 
and locomotion rate were performed by feeding worms with six exopolysaccharide-producing lactobacilli. 
Worms fed with two selected strains were subjected to RNAseq analysis. Identified upregulated genes were 
confirmed by qPCR and expression of their mammalian orthologs checked in human HepG2 cell.
Results: Two strains of lactobacilli showed the most pronounced effect on worms’ lifespan. RNAseq analysis 
identified core gene signature associate with exopolysaccharide-induced longevity. The expression of 
identified fmo-2, gsto-1, nlp-29, and clec-47 genes were confirmed by qPCR, while upregulation of FMO-5 
was confirmed in HepG2 cells.
Conclusion: Overall, our results imply that bacteria-derived exopolysaccharides could stimulate longevity-
promoting flavin-containing monooxygenase 2 to regulate lifespan in Caenorhabditis elegans
C3  - 10th ISM World Congress on Targeting Microbiota
T1  - EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING
EP  - 65
SP  - 65
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2189
ER  - 

@conference{
author = "Dinić, Miroslav and Jakovljević, Stefan and Radojević, Dušan and Brdarić, Emilija and Bajić, Svetlana and Đokić, Jelena and Golić, Nataša",
year = "2023",
abstract = "Introduction: New theories of ageing recognized gut microbiota as one of twelve hallmarks of ageing (1). 
Recent data conducted on Caenorhabditis elegans imply a potential role of Lactobacillus species and other 
commensal bacteria 
in regulation of ageing highlighting TFEB/HLH-30-dependent autophagy, p38 
MAPK/PMK-1 signalling and mitochondrial function as activated longevity-associated mechanisms (2,3). 
Here, we explore the potential of bacterial polysaccharides 
loosely attached to bacterial cell wall 
(exopolysaccharides), considering it is still unknown which bacterial molecules could activate longevity 
signalling pathways.
Materials & Methods: Caenorhabditis elegans was used as ageing model. Evaluation of worm’s lifespan 
and locomotion rate were performed by feeding worms with six exopolysaccharide-producing lactobacilli. 
Worms fed with two selected strains were subjected to RNAseq analysis. Identified upregulated genes were 
confirmed by qPCR and expression of their mammalian orthologs checked in human HepG2 cell.
Results: Two strains of lactobacilli showed the most pronounced effect on worms’ lifespan. RNAseq analysis 
identified core gene signature associate with exopolysaccharide-induced longevity. The expression of 
identified fmo-2, gsto-1, nlp-29, and clec-47 genes were confirmed by qPCR, while upregulation of FMO-5 
was confirmed in HepG2 cells.
Conclusion: Overall, our results imply that bacteria-derived exopolysaccharides could stimulate longevity-
promoting flavin-containing monooxygenase 2 to regulate lifespan in Caenorhabditis elegans",
journal = "10th ISM World Congress on Targeting Microbiota",
title = "EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING",
pages = "65-65",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2189"
}

Dinić, M., Jakovljević, S., Radojević, D., Brdarić, E., Bajić, S., Đokić, J.,& Golić, N.. (2023). EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING. in 10th ISM World Congress on Targeting Microbiota, 65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2189

Dinić M, Jakovljević S, Radojević D, Brdarić E, Bajić S, Đokić J, Golić N. EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING. in 10th ISM World Congress on Targeting Microbiota. 2023;:65-65.
https://hdl.handle.net/21.15107/rcub_imagine_2189 .

Dinić, Miroslav, Jakovljević, Stefan, Radojević, Dušan, Brdarić, Emilija, Bajić, Svetlana, Đokić, Jelena, Golić, Nataša, "EXOPOLYSACCHARIDE-PRODUCING GUT BACTERIA MODULATE HOST AGEING" in 10th ISM World Congress on Targeting Microbiota (2023):65-65,
https://hdl.handle.net/21.15107/rcub_imagine_2189 .

TY  - JOUR
AU  - Radojević, Dušan
AU  - Bajić, Svetlana Soković
AU  - Dinić, Miroslav
AU  - Bisenić, Aleksandar
AU  - Đokić, Jelena
AU  - Golić, Nataša
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/46986
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2294
AB  - The microbiome-gut-brain axis (MGBA) represents a close two-way relationship between the gut and the central nervous system (CNS) mediated by the immune system, the enteric nervous system (ENS), the vagus nerve, and the gut microbiome. Gut microbes, including bacteria, fungi, and viruses, can communicate with the CNS and modulate the physiology of the brain in health and disease, which marks them as an important MGBA factor. It is becoming increasingly evident that gut microbiome dysbiosis is implicated in the onset and severity of different neurodegenerative and psychiatric diseases including multiple sclerosis (MS). MS is a chronic disease of the CNS associated with different genetic and environmental risk factors. Neuroinflammation and demyelination in the brain and the spinal cord are hallmark features of MS. The accumulating evidence shows that the MGBA, although a relatively new concept, has an important role in MS. Therefore, the purpose of this article is to review recent research on the gut-brain connection in MS, and to highlight MS-associated gut microbiota constituents and the role of bacterial metabolites in MS.
AB  - Mikrobiom-crevo-mozak osovina (MGBA) predstavlja blisku dvosmernu vezu između creva i centralnog nervnog sistema (CNS) posredovanu imunskim sistemom, enteričnim nervnim sistemom   (ENS),   nervom   vagusom   i   mikrobiomom   creva.   Posredstvom   metabolita   koje   produkuju, mikroorganizmi creva, uključujući bakterije, gljive i viruse, komuniciraju sa CNS-om i tako utiču na funkcije mozga, zbog čega je mikrobiota creva prepoznata kao veoma važan faktor održavanja homeostaze MGBA. Takođe, veliki broj podataka ukazao je na povezanost disbioze mikrobioma creva i nastanka i težine simptoma različitih neurodegenerativnih i psihijatrijskih bolesti, uključujući multiplu sklerozu (MS), autoimunsku bolest nervnog sistema. MS je hronična bolest  CNS-a  povezana  sa  više  genetskih  faktora, kao i sa različitim sredinskim faktorima i životnim  navikama.  Najvažnija  obeležja  MS  su  neuroinflamacija  i  demijelinizacija  u  mozgu  i  kičmenoj moždini, a veliki broj istraživanja je ukazao i na specifične mikrobijalne markere ove bolesti. Cilj ovog rada je da pruži pregled najvažnijih podataka o povezanosti promena u sastavu i funkciji mikrobiote creva i patoloških promena karakterističnih za MS.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - The Microbiome-Gut-Brain Axis in Multiple Sclerosis
T1  - Mikrobiom-crevo-mozak osovina kod multiple skleroze
EP  - 462
IS  - Notebook 6
SP  - 441
VL  - 73
DO  - 10.5937/arhfarm73-46986
ER  - 

@article{
author = "Radojević, Dušan and Bajić, Svetlana Soković and Dinić, Miroslav and Bisenić, Aleksandar and Đokić, Jelena and Golić, Nataša",
year = "2023",
abstract = "The microbiome-gut-brain axis (MGBA) represents a close two-way relationship between the gut and the central nervous system (CNS) mediated by the immune system, the enteric nervous system (ENS), the vagus nerve, and the gut microbiome. Gut microbes, including bacteria, fungi, and viruses, can communicate with the CNS and modulate the physiology of the brain in health and disease, which marks them as an important MGBA factor. It is becoming increasingly evident that gut microbiome dysbiosis is implicated in the onset and severity of different neurodegenerative and psychiatric diseases including multiple sclerosis (MS). MS is a chronic disease of the CNS associated with different genetic and environmental risk factors. Neuroinflammation and demyelination in the brain and the spinal cord are hallmark features of MS. The accumulating evidence shows that the MGBA, although a relatively new concept, has an important role in MS. Therefore, the purpose of this article is to review recent research on the gut-brain connection in MS, and to highlight MS-associated gut microbiota constituents and the role of bacterial metabolites in MS., Mikrobiom-crevo-mozak osovina (MGBA) predstavlja blisku dvosmernu vezu između creva i centralnog nervnog sistema (CNS) posredovanu imunskim sistemom, enteričnim nervnim sistemom   (ENS),   nervom   vagusom   i   mikrobiomom   creva.   Posredstvom   metabolita   koje   produkuju, mikroorganizmi creva, uključujući bakterije, gljive i viruse, komuniciraju sa CNS-om i tako utiču na funkcije mozga, zbog čega je mikrobiota creva prepoznata kao veoma važan faktor održavanja homeostaze MGBA. Takođe, veliki broj podataka ukazao je na povezanost disbioze mikrobioma creva i nastanka i težine simptoma različitih neurodegenerativnih i psihijatrijskih bolesti, uključujući multiplu sklerozu (MS), autoimunsku bolest nervnog sistema. MS je hronična bolest  CNS-a  povezana  sa  više  genetskih  faktora, kao i sa različitim sredinskim faktorima i životnim  navikama.  Najvažnija  obeležja  MS  su  neuroinflamacija  i  demijelinizacija  u  mozgu  i  kičmenoj moždini, a veliki broj istraživanja je ukazao i na specifične mikrobijalne markere ove bolesti. Cilj ovog rada je da pruži pregled najvažnijih podataka o povezanosti promena u sastavu i funkciji mikrobiote creva i patoloških promena karakterističnih za MS.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "The Microbiome-Gut-Brain Axis in Multiple Sclerosis, Mikrobiom-crevo-mozak osovina kod multiple skleroze",
pages = "462-441",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-46986"
}

Radojević, D., Bajić, S. S., Dinić, M., Bisenić, A., Đokić, J.,& Golić, N.. (2023). The Microbiome-Gut-Brain Axis in Multiple Sclerosis. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 441-462.
https://doi.org/10.5937/arhfarm73-46986

Radojević D, Bajić SS, Dinić M, Bisenić A, Đokić J, Golić N. The Microbiome-Gut-Brain Axis in Multiple Sclerosis. in Archives of Pharmacy. 2023;73(Notebook 6):441-462.
doi:10.5937/arhfarm73-46986 .

Radojević, Dušan, Bajić, Svetlana Soković, Dinić, Miroslav, Bisenić, Aleksandar, Đokić, Jelena, Golić, Nataša, "The Microbiome-Gut-Brain Axis in Multiple Sclerosis" in Archives of Pharmacy, 73, no. Notebook 6 (2023):441-462,
https://doi.org/10.5937/arhfarm73-46986 . .

TY  - CONF
AU  - Mesaroš, Aleksandra
AU  - Atanasković, Iva
AU  - Jakovljević, Stefan
AU  - Stević, Tatjana
AU  - Dinić, Miroslav
AU  - Lozo, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2152
AB  - Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Determination of hydrogen cyanide producing strains as potential biocontrol agents
EP  - 116
SP  - 116
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2152
ER  - 

@conference{
author = "Mesaroš, Aleksandra and Atanasković, Iva and Jakovljević, Stefan and Stević, Tatjana and Dinić, Miroslav and Lozo, Jelena",
year = "2023",
abstract = "Introduction: Hydrogen cyanide (HCN) is a volatile secondary metabolite synthesized by some bacteria, and this ability enablestheir activity against various pathogens. The aim of thisstudy wasto identify
HCN-producing bacteria and investigate their biocontrol potential.
Methods: Three HCN-producing strains were detected in a collection of bell pepper plant isolates using
a semi-quantitative assay with picric acid. The presence of hcnABC operon genes was confirmed by PCR.
The biological control potential of the HCN-producing strains wastested against three fungal (Fusarium
oxysporum, Rhizoctonia solani, Verticillium dahliae) and eight bacterial (genera Xanthomonas,
Pseudomonas and Clavibacter) pathogens of bell pepper plants in a split-section Petri dish experiment.
The potential nematocidal activity was demonstrated by using the Caenorhabditis elegans AU37 strain,
with temperature-sensitive sterility and enhanced sensitivity to pathogens.
Results: Detailed characterization of 300 isolates from our collection revealed that we have three different HCN-producing strains identified as Bacillussubtilis, Pseudomonas moraviensis, and P. putida, with
P. putida A32 being the most potent. Thisstrain is used for the deletion of the hcnB gene to confirm HCN
as a biocontrol agent.
Conclusion: The HCN-producing strains showed biocontrol potential against bacteria, fungi, and nematodes. It is concluded that the biological control activity isthe result of a volatile metabolite diffusing
through the air. Our future exp",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Determination of hydrogen cyanide producing strains as potential biocontrol agents",
pages = "116-116",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2152"
}

Mesaroš, A., Atanasković, I., Jakovljević, S., Stević, T., Dinić, M.,& Lozo, J.. (2023). Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2152

Mesaroš A, Atanasković I, Jakovljević S, Stević T, Dinić M, Lozo J. Determination of hydrogen cyanide producing strains as potential biocontrol agents. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:116-116.
https://hdl.handle.net/21.15107/rcub_imagine_2152 .

Mesaroš, Aleksandra, Atanasković, Iva, Jakovljević, Stefan, Stević, Tatjana, Dinić, Miroslav, Lozo, Jelena, "Determination of hydrogen cyanide producing strains as potential biocontrol agents" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):116-116,
https://hdl.handle.net/21.15107/rcub_imagine_2152 .

TY  - CONF
AU  - Dinić, Miroslav
AU  - Bisenić, Aleksandar
AU  - Jakovljević, Stefan
AU  - Nastasijević, Branislav
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Terzić-Vidojević, Amarela
AU  - Golić, Nataša
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2129
AB  - Introduction: Gut-brain axis has been identified as an important target for prevention of neurodegenerative and psychiatric disease. To date, specific microbial strains resident in the intestinal ecosystem
have been described to modulate several behaviour-related functions in the host. Faecalimonas sp. is
anaerobic bacteria affiliated with the family Lachnospiraceae, which represents a highly prevalent beneficial bacteria in the human gut and have potential to be used as next generation probiotic.
Methods: Faecalimonas sp. NGB245 was isolated from human fecal material by pre-inoculation in
BACTEC media followed by serial dilutions spreading on Columbia Blood Agar supplemented with cysteine and sodium thioglycolate in Whitley Anaerobic Workstation. Production of short chain fatty acid
(SCFA) was detected after bacterial growth in Columbia broth supplemented with cellobiose by HPLC.
Host response was followed on Caenorhabditis elegans model by evaluated expression of the genes involved in neurosignaling by qPCR.
Results: We showed that Faecalimonassp. NGB245 exhibits high capacity of production of SCFA including acetate (12,17 mM), propionate (3,02 mM) and butyrate (10,33 mM). Moreover, C. elegansfed with Faecalimonas sp. NGB245 showed higher expression of the genes involved in neurotransmitter synthesis
(tph-1, cat-2), neurotransmitter release (unc-64, snb-1, snt-1), neurotransmitter receptor (npr-1) and different classes of neuropeptides(flp-18, flp-21, nlp-28, nlp-29) in comparison to wormsfed with Escherichia
coli OP50, as a standard laboratory food.
Conclusion: The obtained results imply that Faecalimonas sp. NGB245 isolate could be considered as
next generation probiotic to be used in prevention and treatment of neurodegenerative and psychiatric
diseases.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans
EP  - 124
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2129
ER  - 

@conference{
author = "Dinić, Miroslav and Bisenić, Aleksandar and Jakovljević, Stefan and Nastasijević, Branislav and Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Terzić-Vidojević, Amarela and Golić, Nataša",
year = "2023",
abstract = "Introduction: Gut-brain axis has been identified as an important target for prevention of neurodegenerative and psychiatric disease. To date, specific microbial strains resident in the intestinal ecosystem
have been described to modulate several behaviour-related functions in the host. Faecalimonas sp. is
anaerobic bacteria affiliated with the family Lachnospiraceae, which represents a highly prevalent beneficial bacteria in the human gut and have potential to be used as next generation probiotic.
Methods: Faecalimonas sp. NGB245 was isolated from human fecal material by pre-inoculation in
BACTEC media followed by serial dilutions spreading on Columbia Blood Agar supplemented with cysteine and sodium thioglycolate in Whitley Anaerobic Workstation. Production of short chain fatty acid
(SCFA) was detected after bacterial growth in Columbia broth supplemented with cellobiose by HPLC.
Host response was followed on Caenorhabditis elegans model by evaluated expression of the genes involved in neurosignaling by qPCR.
Results: We showed that Faecalimonassp. NGB245 exhibits high capacity of production of SCFA including acetate (12,17 mM), propionate (3,02 mM) and butyrate (10,33 mM). Moreover, C. elegansfed with Faecalimonas sp. NGB245 showed higher expression of the genes involved in neurotransmitter synthesis
(tph-1, cat-2), neurotransmitter release (unc-64, snb-1, snt-1), neurotransmitter receptor (npr-1) and different classes of neuropeptides(flp-18, flp-21, nlp-28, nlp-29) in comparison to wormsfed with Escherichia
coli OP50, as a standard laboratory food.
Conclusion: The obtained results imply that Faecalimonas sp. NGB245 isolate could be considered as
next generation probiotic to be used in prevention and treatment of neurodegenerative and psychiatric
diseases.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans",
pages = "124-124",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2129"
}

Dinić, M., Bisenić, A., Jakovljević, S., Nastasijević, B., Brdarić, E., Soković Bajić, S., Đokić, J., Terzić-Vidojević, A.,& Golić, N.. (2023). Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 124-124.
https://hdl.handle.net/21.15107/rcub_imagine_2129

Dinić M, Bisenić A, Jakovljević S, Nastasijević B, Brdarić E, Soković Bajić S, Đokić J, Terzić-Vidojević A, Golić N. Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:124-124.
https://hdl.handle.net/21.15107/rcub_imagine_2129 .

Dinić, Miroslav, Bisenić, Aleksandar, Jakovljević, Stefan, Nastasijević, Branislav, Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Terzić-Vidojević, Amarela, Golić, Nataša, "Short chain fatty acid producing faecalimonas sp. NGB245 isolated from human gut modulates neurosignaling in Caenorhabditis elegans" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):124-124,
https://hdl.handle.net/21.15107/rcub_imagine_2129 .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Veljović, Katarina
AU  - Golić, Nataša
AU  - Terzić-Vidojević, Amarela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2091
AB  - Yogurt represent one of the oldest fermented foods containing viable lactic acid bacteria and many bioactive compounds that could exhibit beneficial effects on human health and train our immune system to better respond to invading pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are commonly used for yogurt preparation under controlled temperature and environmental conditions. In this study, we investigated probiotic features of S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains isolated from artisanal sour milk and yogurt by using Caenorhabditis elegans as an in vivo model system. Further, we evaluated content of total fat, saturated fatty acids, proteins, and lactose, as well as vitamins and AA of yogurt prepared from above-mentioned starter cultures during 21 d of storage at 4°C to get insights of final product stability. We showed that S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains applied in combination upregulated the expression of autophagy-related genes in C. elegans. Beside autophagy, we observed activation of TIR-1-dependent transcription of lysozyme-like antimicrobial genes involved in the immune defense of C. elegans. Upregulation of these genes strongly correlates with an increase in the longevity of the worms fed with yogurt culture bacteria. Further, we showed that yogurt prepared with S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21, as a final product, is rich with vitamin B2 and dominant AA known by their prolongevity properties. Taken together, our study pointed to the beneficial features of the tested starter cultures and yogurt and highlighted their potential to be used as a fermented food with added-value properties.
PB  - Elsevier
PB  - American Dairy Science Association
T2  - Journal of Dairy Science
T2  - Journal of Dairy ScienceJournal of Dairy Science
T1  - Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans
EP  - 7460
IS  - 11
SP  - 7447
VL  - 106
DO  - 10.3168/jds.2023-23342
ER  - 

@article{
author = "Dinić, Miroslav and Jakovljević, Stefan and Popović, Nikola and Radojević, Dušan and Veljović, Katarina and Golić, Nataša and Terzić-Vidojević, Amarela",
year = "2023",
abstract = "Yogurt represent one of the oldest fermented foods containing viable lactic acid bacteria and many bioactive compounds that could exhibit beneficial effects on human health and train our immune system to better respond to invading pathogens. Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus are commonly used for yogurt preparation under controlled temperature and environmental conditions. In this study, we investigated probiotic features of S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains isolated from artisanal sour milk and yogurt by using Caenorhabditis elegans as an in vivo model system. Further, we evaluated content of total fat, saturated fatty acids, proteins, and lactose, as well as vitamins and AA of yogurt prepared from above-mentioned starter cultures during 21 d of storage at 4°C to get insights of final product stability. We showed that S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21 strains applied in combination upregulated the expression of autophagy-related genes in C. elegans. Beside autophagy, we observed activation of TIR-1-dependent transcription of lysozyme-like antimicrobial genes involved in the immune defense of C. elegans. Upregulation of these genes strongly correlates with an increase in the longevity of the worms fed with yogurt culture bacteria. Further, we showed that yogurt prepared with S. thermophilus BGKMJ1-36 and L. bulgaricus BGVLJ1-21, as a final product, is rich with vitamin B2 and dominant AA known by their prolongevity properties. Taken together, our study pointed to the beneficial features of the tested starter cultures and yogurt and highlighted their potential to be used as a fermented food with added-value properties.",
publisher = "Elsevier, American Dairy Science Association",
journal = "Journal of Dairy Science, Journal of Dairy ScienceJournal of Dairy Science",
title = "Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans",
pages = "7460-7447",
number = "11",
volume = "106",
doi = "10.3168/jds.2023-23342"
}

Dinić, M., Jakovljević, S., Popović, N., Radojević, D., Veljović, K., Golić, N.,& Terzić-Vidojević, A.. (2023). Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans. in Journal of Dairy Science
Elsevier., 106(11), 7447-7460.
https://doi.org/10.3168/jds.2023-23342

Dinić M, Jakovljević S, Popović N, Radojević D, Veljović K, Golić N, Terzić-Vidojević A. Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans. in Journal of Dairy Science. 2023;106(11):7447-7460.
doi:10.3168/jds.2023-23342 .

Dinić, Miroslav, Jakovljević, Stefan, Popović, Nikola, Radojević, Dušan, Veljović, Katarina, Golić, Nataša, Terzić-Vidojević, Amarela, "Stability and bioactive compounds assessment of yogurt containing novel natural starter cultures with the ability to promote longevity in Caenorhabditis elegans" in Journal of Dairy Science, 106, no. 11 (2023):7447-7460,
https://doi.org/10.3168/jds.2023-23342 . .

TY  - CONF
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Radojević, Dušan
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Golić, Nataša
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2146
AB  - Introduction: Microbial community established in the gut has been recognized as an important factor
which influence host aging. Bacteria from the gut co-evolved with the host resulting in mutually beneficial interactions essential for host’s wellbeing. This complex crosstalk reflects mainly through the interaction between bacterial macromolecules (e.g., exopolysaccharides) and the host receptors leading
to the activation of various cellular pathways. Here, we explore the potential of different lactobacilli,
commonly used as probiotics, to activate longevity signalling in Caenorhabditis elegans.
Methods: Evaluation of C. elegans lifespan and aging parameters (locomotion rate and pharyngeal
pumping) were performed by feeding N2 wild-type worms with different Lactobacillus species. Worms
fed with selected strains were subjected to RNAseq analysis, qPCR and Western blot to evaluate activation of autophagy, immunity, antioxidative response and mitochondrial function. Activation of autophagy was confirmed in DA2123 GFP-labelled LGG-1 transgenic strain and JIN1375 hlh-30 (tm1978)
mutant, while immunity activation was evaluated by using KU25 pmk-1 (km25) mutant and through
nematode killing assays.
Results: Selected strains of lactobacilli promoted health and lifespan of worms through activation of
TFEB/HLH-30 dependent autophagy and p38 MAPK/PMK-1 dependent immune response which provided resistance of worms exposed to pathogens. Moreover, RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity highlighting involvement of fmo-2, gsto-1,
nlp-29, and clec-47 genes in increased lifespan of the worms.
Conclusion: Analyzed lactobacilli showed potential to promote healthy aging and could be further investigated in order to better understand application of lactobacilli as pro-longevity probiotics.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - The role of the gut bacteria during host aging
EP  - 108
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2146
ER  - 

@conference{
author = "Dinić, Miroslav and Jakovljević, Stefan and Radojević, Dušan and Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Golić, Nataša",
year = "2023",
abstract = "Introduction: Microbial community established in the gut has been recognized as an important factor
which influence host aging. Bacteria from the gut co-evolved with the host resulting in mutually beneficial interactions essential for host’s wellbeing. This complex crosstalk reflects mainly through the interaction between bacterial macromolecules (e.g., exopolysaccharides) and the host receptors leading
to the activation of various cellular pathways. Here, we explore the potential of different lactobacilli,
commonly used as probiotics, to activate longevity signalling in Caenorhabditis elegans.
Methods: Evaluation of C. elegans lifespan and aging parameters (locomotion rate and pharyngeal
pumping) were performed by feeding N2 wild-type worms with different Lactobacillus species. Worms
fed with selected strains were subjected to RNAseq analysis, qPCR and Western blot to evaluate activation of autophagy, immunity, antioxidative response and mitochondrial function. Activation of autophagy was confirmed in DA2123 GFP-labelled LGG-1 transgenic strain and JIN1375 hlh-30 (tm1978)
mutant, while immunity activation was evaluated by using KU25 pmk-1 (km25) mutant and through
nematode killing assays.
Results: Selected strains of lactobacilli promoted health and lifespan of worms through activation of
TFEB/HLH-30 dependent autophagy and p38 MAPK/PMK-1 dependent immune response which provided resistance of worms exposed to pathogens. Moreover, RNAseq analysis identified core gene signature associate with exopolysaccharide-induced longevity highlighting involvement of fmo-2, gsto-1,
nlp-29, and clec-47 genes in increased lifespan of the worms.
Conclusion: Analyzed lactobacilli showed potential to promote healthy aging and could be further investigated in order to better understand application of lactobacilli as pro-longevity probiotics.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "The role of the gut bacteria during host aging",
pages = "108-108",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2146"
}

Dinić, M., Jakovljević, S., Radojević, D., Brdarić, E., Soković Bajić, S., Đokić, J.,& Golić, N.. (2023). The role of the gut bacteria during host aging. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 108-108.
https://hdl.handle.net/21.15107/rcub_imagine_2146

Dinić M, Jakovljević S, Radojević D, Brdarić E, Soković Bajić S, Đokić J, Golić N. The role of the gut bacteria during host aging. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:108-108.
https://hdl.handle.net/21.15107/rcub_imagine_2146 .

Dinić, Miroslav, Jakovljević, Stefan, Radojević, Dušan, Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Golić, Nataša, "The role of the gut bacteria during host aging" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):108-108,
https://hdl.handle.net/21.15107/rcub_imagine_2146 .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Đokić, Jelena
PY  - 2023
UR  - https://aseestant.ceon.rs/index.php/arhfarm/article/view/46614
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2297
AB  - Probiotic lactobacilli exhibit the potential to promote health benefits for the host. Thanks to its numerous beneficial effects on human health, Limosilactobacillus fermentum stood out as an excellent candidate for the development of commercial probiotic preparations aiming to prevent community-acquired infections. In this study, several in vitro tests, including biofilm formation assay, assessment of antibiotic susceptibility, survival in simulated gastrointestinal tract conditions and attachment to intestinal Caco-2 cells, were used to estimate the safety and probiotic potential of L. fermentum BGHV110 strain. Additionally, Caenorhabditis elegans was used as an in vivo model system for the evaluation of L. fermentum BGHV110 influence on the host’s innate immune response. The results revealed that L. fermentum BGHV110 strain showed an excellent capability to survive harsh conditions of the gut, to attach to intestinal Caco-2 cells and to stimulate conserved p38 MAPK immunity pathway and expression of the clc-1 claudin-like gene and antimicrobial peptides in C. elegans in order to enhance the immune response against pathogens. Finally, L. fermentum BGHV110 showed no virulence traits and susceptibility to tested antibiotics, confirming its safety status which enables it to be applied as a future probiotic.
PB  - Centre for Evaluation in Education and Science
T2  - Archives of Pharmacy
T1  - Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans
T1  - Probiotička karakterizacija soja Limosilactobacillus fermentum BGHV110 i njegov uticaj na urođeni imunski odgovor kod Caenorhabditis elegans
EP  - 585
IS  - Notebook 6
SP  - 571
VL  - 73
DO  - 10.5937/arhfarm73-46614
ER  - 

@article{
author = "Dinić, Miroslav and Popović, Nikola and Radojević, Dušan and Đokić, Jelena",
year = "2023",
abstract = "Probiotic lactobacilli exhibit the potential to promote health benefits for the host. Thanks to its numerous beneficial effects on human health, Limosilactobacillus fermentum stood out as an excellent candidate for the development of commercial probiotic preparations aiming to prevent community-acquired infections. In this study, several in vitro tests, including biofilm formation assay, assessment of antibiotic susceptibility, survival in simulated gastrointestinal tract conditions and attachment to intestinal Caco-2 cells, were used to estimate the safety and probiotic potential of L. fermentum BGHV110 strain. Additionally, Caenorhabditis elegans was used as an in vivo model system for the evaluation of L. fermentum BGHV110 influence on the host’s innate immune response. The results revealed that L. fermentum BGHV110 strain showed an excellent capability to survive harsh conditions of the gut, to attach to intestinal Caco-2 cells and to stimulate conserved p38 MAPK immunity pathway and expression of the clc-1 claudin-like gene and antimicrobial peptides in C. elegans in order to enhance the immune response against pathogens. Finally, L. fermentum BGHV110 showed no virulence traits and susceptibility to tested antibiotics, confirming its safety status which enables it to be applied as a future probiotic.",
publisher = "Centre for Evaluation in Education and Science",
journal = "Archives of Pharmacy",
title = "Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans, Probiotička karakterizacija soja Limosilactobacillus fermentum BGHV110 i njegov uticaj na urođeni imunski odgovor kod Caenorhabditis elegans",
pages = "585-571",
number = "Notebook 6",
volume = "73",
doi = "10.5937/arhfarm73-46614"
}

Dinić, M., Popović, N., Radojević, D.,& Đokić, J.. (2023). Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans. in Archives of Pharmacy
Centre for Evaluation in Education and Science., 73(Notebook 6), 571-585.
https://doi.org/10.5937/arhfarm73-46614

Dinić M, Popović N, Radojević D, Đokić J. Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans. in Archives of Pharmacy. 2023;73(Notebook 6):571-585.
doi:10.5937/arhfarm73-46614 .

Dinić, Miroslav, Popović, Nikola, Radojević, Dušan, Đokić, Jelena, "Probiotic characterization of Limosilactobacillus fermentum BGHV110 strain and its influence on innate immune response in Caenorhabditis elegans" in Archives of Pharmacy, 73, no. Notebook 6 (2023):571-585,
https://doi.org/10.5937/arhfarm73-46614 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Gardijan, Lazar
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1525
AB  - Signal transduction systems are the key players of bacterial adaptation and survival. The orthodox two-component signal transduction systems perceive diverse environmental stimuli and their regulatory response leads to cellular changes. Although rarely described, the unorthodox three-component systems are also implemented in the regulation of major bacterial behavior such as the virulence of clinically relevant pathogen P. aeruginosa. Previously, we described a novel three-component system in P. capeferrum WCS358 (RclSAR) where the sensor kinase RclS stimulates the intI1 transcription in stationary growth phase. In this study, using rclS knock-out mutant, we identified RclSAR regulon in P. capeferrum WCS358. The RNA sequencing revealed that activity of RclSAR signal transduction system is growth phase dependent with more pronounced regulatory potential in early stages of growth. Transcriptional analysis emphasized the role of RclSAR in global regulation and indicated the involvement of this system in regulation of diverse cellular activities such as RNA binding and metabolic and biocontrol processes. Importantly, phenotypic comparison of WCS358 wild type and Delta rclS mutant showed that RclS sensor kinase contributes to modulation of antibiotic resistance, production of AHLs and siderophore as well as host cell adherence and cytotoxicity. Finally, we proposed the improved model of interplay between RclSAR, RpoS and LasIR regulatory systems in P. capeferrum WCS358.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum
IS  - 15
VL  - 23
DO  - 10.3390/ijms23158232
ER  - 

@article{
author = "Novović, Katarina and Malešević, Milka and Dinić, Miroslav and Gardijan, Lazar and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "Signal transduction systems are the key players of bacterial adaptation and survival. The orthodox two-component signal transduction systems perceive diverse environmental stimuli and their regulatory response leads to cellular changes. Although rarely described, the unorthodox three-component systems are also implemented in the regulation of major bacterial behavior such as the virulence of clinically relevant pathogen P. aeruginosa. Previously, we described a novel three-component system in P. capeferrum WCS358 (RclSAR) where the sensor kinase RclS stimulates the intI1 transcription in stationary growth phase. In this study, using rclS knock-out mutant, we identified RclSAR regulon in P. capeferrum WCS358. The RNA sequencing revealed that activity of RclSAR signal transduction system is growth phase dependent with more pronounced regulatory potential in early stages of growth. Transcriptional analysis emphasized the role of RclSAR in global regulation and indicated the involvement of this system in regulation of diverse cellular activities such as RNA binding and metabolic and biocontrol processes. Importantly, phenotypic comparison of WCS358 wild type and Delta rclS mutant showed that RclS sensor kinase contributes to modulation of antibiotic resistance, production of AHLs and siderophore as well as host cell adherence and cytotoxicity. Finally, we proposed the improved model of interplay between RclSAR, RpoS and LasIR regulatory systems in P. capeferrum WCS358.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum",
number = "15",
volume = "23",
doi = "10.3390/ijms23158232"
}

Novović, K., Malešević, M., Dinić, M., Gardijan, L., Kojić, M.,& Jovčić, B.. (2022). RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum. in International Journal of Molecular Sciences
MDPI, Basel., 23(15).
https://doi.org/10.3390/ijms23158232

Novović K, Malešević M, Dinić M, Gardijan L, Kojić M, Jovčić B. RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum. in International Journal of Molecular Sciences. 2022;23(15).
doi:10.3390/ijms23158232 .

Novović, Katarina, Malešević, Milka, Dinić, Miroslav, Gardijan, Lazar, Kojić, Milan, Jovčić, Branko, "RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum" in International Journal of Molecular Sciences, 23, no. 15 (2022),
https://doi.org/10.3390/ijms23158232 . .

TY  - JOUR
AU  - Radojević, Dušan
AU  - Bekić, Marina
AU  - Gruden-Movsesijan, Alisa
AU  - Ilić, Nataša
AU  - Dinić, Miroslav
AU  - Bisenić, Aleksandar
AU  - Golić, Nataša
AU  - Vucević, Dragana
AU  - Đokić, Jelena
AU  - Tomić, Sergej
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1540
AB  - Over-activated myeloid cells and disturbance in gut microbiota composition are critical factors contributing to the pathogenesis of Multiple Sclerosis (MS). Myeloid-derived suppressor cells (MDSCs) emerged as promising regulators of chronic inflammatory diseases, including autoimmune diseases. However, it remained unclear whether MDSCs display any therapeutic potential in MS, and how this therapy modulates gut microbiota composition. Here, we assessed the potential of in vitro generated bone marrow-derived MDSCs to ameliorate experimental autoimmune encephalomyelitis (EAE) in Dark Agouti rats and investigated how their application associates with the changes in gut microbiota composition. MDSCs differentiated with prostaglandin (PG)E2 (MDSC-PGE2) and control MDSCs (differentiated without PGE2) displayed strong immunosuppressive properties in vitro, but only MDSC-PGE2 significantly ameliorated EAE symptoms. This effect correlated with a reduced infiltration of Th17 and IFN-gamma-producing NK cells, and an increased proportion of regulatory T cells in the CNS and spleen. Importantly, both MDSCs and MDSC-PGE2 prevented EAE-induced reduction of gut microbiota diversity, but only MDSC-PGE2 prevented the extensive alterations in gut microbiota composition following their early migration into Payer's patches and mesenteric lymph nodes. This phenomenon was related to the significant enrichment of gut microbial taxa with potential immunoregulatory properties, as well as higher levels of butyrate, propionate, and putrescine in feces. This study provides new insights into the host-microbiota interactions in EAE, suggesting that activated MDSCs could be potentially used as an efficient therapy for acute phases of MS. Considering a significant association between the efficacy of MDSC-PGE2 and gut microbiota composition, our findings also provide a rationale for further exploring the specific microbial metabolites in MS therapy.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Gut Microbes
T1  - Myeloid-derived suppressor cells prevent disruption of the gut barrier, preserve microbiota composition, and potentiate immunoregulatory pathways in a rat model of experimental autoimmune encephalomyelitis
IS  - 1
VL  - 14
DO  - 10.1080/19490976.2022.2127455
ER  - 

@article{
author = "Radojević, Dušan and Bekić, Marina and Gruden-Movsesijan, Alisa and Ilić, Nataša and Dinić, Miroslav and Bisenić, Aleksandar and Golić, Nataša and Vucević, Dragana and Đokić, Jelena and Tomić, Sergej",
year = "2022",
abstract = "Over-activated myeloid cells and disturbance in gut microbiota composition are critical factors contributing to the pathogenesis of Multiple Sclerosis (MS). Myeloid-derived suppressor cells (MDSCs) emerged as promising regulators of chronic inflammatory diseases, including autoimmune diseases. However, it remained unclear whether MDSCs display any therapeutic potential in MS, and how this therapy modulates gut microbiota composition. Here, we assessed the potential of in vitro generated bone marrow-derived MDSCs to ameliorate experimental autoimmune encephalomyelitis (EAE) in Dark Agouti rats and investigated how their application associates with the changes in gut microbiota composition. MDSCs differentiated with prostaglandin (PG)E2 (MDSC-PGE2) and control MDSCs (differentiated without PGE2) displayed strong immunosuppressive properties in vitro, but only MDSC-PGE2 significantly ameliorated EAE symptoms. This effect correlated with a reduced infiltration of Th17 and IFN-gamma-producing NK cells, and an increased proportion of regulatory T cells in the CNS and spleen. Importantly, both MDSCs and MDSC-PGE2 prevented EAE-induced reduction of gut microbiota diversity, but only MDSC-PGE2 prevented the extensive alterations in gut microbiota composition following their early migration into Payer's patches and mesenteric lymph nodes. This phenomenon was related to the significant enrichment of gut microbial taxa with potential immunoregulatory properties, as well as higher levels of butyrate, propionate, and putrescine in feces. This study provides new insights into the host-microbiota interactions in EAE, suggesting that activated MDSCs could be potentially used as an efficient therapy for acute phases of MS. Considering a significant association between the efficacy of MDSC-PGE2 and gut microbiota composition, our findings also provide a rationale for further exploring the specific microbial metabolites in MS therapy.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Gut Microbes",
title = "Myeloid-derived suppressor cells prevent disruption of the gut barrier, preserve microbiota composition, and potentiate immunoregulatory pathways in a rat model of experimental autoimmune encephalomyelitis",
number = "1",
volume = "14",
doi = "10.1080/19490976.2022.2127455"
}

Radojević, D., Bekić, M., Gruden-Movsesijan, A., Ilić, N., Dinić, M., Bisenić, A., Golić, N., Vucević, D., Đokić, J.,& Tomić, S.. (2022). Myeloid-derived suppressor cells prevent disruption of the gut barrier, preserve microbiota composition, and potentiate immunoregulatory pathways in a rat model of experimental autoimmune encephalomyelitis. in Gut Microbes
Taylor & Francis Inc, Philadelphia., 14(1).
https://doi.org/10.1080/19490976.2022.2127455

Radojević D, Bekić M, Gruden-Movsesijan A, Ilić N, Dinić M, Bisenić A, Golić N, Vucević D, Đokić J, Tomić S. Myeloid-derived suppressor cells prevent disruption of the gut barrier, preserve microbiota composition, and potentiate immunoregulatory pathways in a rat model of experimental autoimmune encephalomyelitis. in Gut Microbes. 2022;14(1).
doi:10.1080/19490976.2022.2127455 .

Radojević, Dušan, Bekić, Marina, Gruden-Movsesijan, Alisa, Ilić, Nataša, Dinić, Miroslav, Bisenić, Aleksandar, Golić, Nataša, Vucević, Dragana, Đokić, Jelena, Tomić, Sergej, "Myeloid-derived suppressor cells prevent disruption of the gut barrier, preserve microbiota composition, and potentiate immunoregulatory pathways in a rat model of experimental autoimmune encephalomyelitis" in Gut Microbes, 14, no. 1 (2022),
https://doi.org/10.1080/19490976.2022.2127455 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Stojković, Dejan
AU  - Jovčić, Branko
AU  - Soković, Marina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1566
AB  - The rising incidence of antibiotic resistant microorganisms urges novel antimicrobials development with polyphenols as appealing potential therapeutics. We aimed to reveal the most promising polyphenols among hesperetin, hesperidin, naringenin, naringin, taxifolin, rutin, isoquercitrin, morin, chlorogenic acid, ferulic acid, p-coumaric acid, and gallic acid based on antimicrobial capacity, antibiofilm potential, and lack of cytotoxicity towards HaCaT, and to further test its antivirulence mechanisms. Although the majority of studied polyphenols were able to inhibit bacterial growth and biofilm formation, the most promising activities were observed for rutin. Further investigation proved rutin's ability to prevent/eradicate Pseudomonas aeruginosa and MRSA urinary catheter biofilms. Besides reduction of biofilm biomass, rutin antibiofilm mechanisms included reduction of cell viability, exopolysaccharide, and extracellular DNA levels. Moderate reduction of bacterial adhesion to human keratinocytes upon treatment was observed. Rutin antivirulence mechanisms included an impact on P. aeruginosa protease, pyocyanin, rhamnolipid, and elastase production and the downregulation of the lasI, lasR, rhlI, rhlR, pqsA and mvfR genes. Rutin also interfered with membrane permeability. Polyphenols could repress antibiotic resistant bacteria. Rutin has shown wide antimicrobial and antibiofilm capacity employing a range of mechanisms that might be used for the development of novel antimicrobials.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence
IS  - 3
VL  - 15
DO  - 10.3390/ph15030385
ER  - 

@article{
author = "Ivanov, Marija and Novović, Katarina and Malešević, Milka and Dinić, Miroslav and Stojković, Dejan and Jovčić, Branko and Soković, Marina",
year = "2022",
abstract = "The rising incidence of antibiotic resistant microorganisms urges novel antimicrobials development with polyphenols as appealing potential therapeutics. We aimed to reveal the most promising polyphenols among hesperetin, hesperidin, naringenin, naringin, taxifolin, rutin, isoquercitrin, morin, chlorogenic acid, ferulic acid, p-coumaric acid, and gallic acid based on antimicrobial capacity, antibiofilm potential, and lack of cytotoxicity towards HaCaT, and to further test its antivirulence mechanisms. Although the majority of studied polyphenols were able to inhibit bacterial growth and biofilm formation, the most promising activities were observed for rutin. Further investigation proved rutin's ability to prevent/eradicate Pseudomonas aeruginosa and MRSA urinary catheter biofilms. Besides reduction of biofilm biomass, rutin antibiofilm mechanisms included reduction of cell viability, exopolysaccharide, and extracellular DNA levels. Moderate reduction of bacterial adhesion to human keratinocytes upon treatment was observed. Rutin antivirulence mechanisms included an impact on P. aeruginosa protease, pyocyanin, rhamnolipid, and elastase production and the downregulation of the lasI, lasR, rhlI, rhlR, pqsA and mvfR genes. Rutin also interfered with membrane permeability. Polyphenols could repress antibiotic resistant bacteria. Rutin has shown wide antimicrobial and antibiofilm capacity employing a range of mechanisms that might be used for the development of novel antimicrobials.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence",
number = "3",
volume = "15",
doi = "10.3390/ph15030385"
}

Ivanov, M., Novović, K., Malešević, M., Dinić, M., Stojković, D., Jovčić, B.,& Soković, M.. (2022). Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence. in Pharmaceuticals
MDPI, Basel., 15(3).
https://doi.org/10.3390/ph15030385

Ivanov M, Novović K, Malešević M, Dinić M, Stojković D, Jovčić B, Soković M. Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence. in Pharmaceuticals. 2022;15(3).
doi:10.3390/ph15030385 .

Ivanov, Marija, Novović, Katarina, Malešević, Milka, Dinić, Miroslav, Stojković, Dejan, Jovčić, Branko, Soković, Marina, "Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence" in Pharmaceuticals, 15, no. 3 (2022),
https://doi.org/10.3390/ph15030385 . .

TY  - JOUR
AU  - Ljubić, Verica
AU  - Milosević, Milena
AU  - Cvetković, Slobodan
AU  - Stojanović, Marijana
AU  - Novović, Katarina
AU  - Dinić, Miroslav
AU  - Popović, Mina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1523
AB  - As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.
PB  - Springer Heidelberg, Heidelberg
T2  - Biomass Conversion and Biorefinery
T1  - The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water
DO  - 10.1007/s13399-022-03292-5
ER  - 

@article{
author = "Ljubić, Verica and Milosević, Milena and Cvetković, Slobodan and Stojanović, Marijana and Novović, Katarina and Dinić, Miroslav and Popović, Mina",
year = "2022",
abstract = "As one of the most promising groups of microbes, lactic acid bacteria (LAB) can synthesize metabolites that can be used in different industries over the world, mainly in the pharmaceutical, food, and dairy industries. In this study, a novel exopolysaccharide was extracted and isolated from the probiotic strain Lactobacillus reuteri B2, and assessed on biological activity and its possible application as a biosorbent for the removal of Ni2+ ions from contaminated water. New exopolysaccharide was characterized using FTIR, SEM, XRD, NMR, MALDI-TOF MS, and TGA/DTG analysis. Biological assays included antioxidative activity, cytotoxic assay, and adhesion assay of L. reuteri B2 to HT29 cells. Our hypothesis was that if this exopolysaccharide is nontoxic, it can be used as a novel biomaterial for the possible application of the removal of Ni2+ ions from contaminated water. The scavenging effect of nontoxic exopolysaccharide was 76% at 2 mg/mL using ABTS assay, in biological assays, while the removal efficiency of nickel from the aqueous solution was 92.96% in biosorption study. According to these results, this exopolysaccharide can be considered a very promising biomaterial for potential application in different industries, from pharmacy to wastewater treatments.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Biomass Conversion and Biorefinery",
title = "The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water",
doi = "10.1007/s13399-022-03292-5"
}

Ljubić, V., Milosević, M., Cvetković, S., Stojanović, M., Novović, K., Dinić, M.,& Popović, M.. (2022). The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water. in Biomass Conversion and Biorefinery
Springer Heidelberg, Heidelberg..
https://doi.org/10.1007/s13399-022-03292-5

Ljubić V, Milosević M, Cvetković S, Stojanović M, Novović K, Dinić M, Popović M. The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water. in Biomass Conversion and Biorefinery. 2022;.
doi:10.1007/s13399-022-03292-5 .

Ljubić, Verica, Milosević, Milena, Cvetković, Slobodan, Stojanović, Marijana, Novović, Katarina, Dinić, Miroslav, Popović, Mina, "The new exopolysaccharide produced by the probiotic strain L. reuteri B2: extraction, biological properties, and possible application for Ni2+ ion removal from the contaminated water" in Biomass Conversion and Biorefinery (2022),
https://doi.org/10.1007/s13399-022-03292-5 . .

TY  - CONF
AU  - Radojević, Dušan
AU  - Bekić, Marina
AU  - Gruden-Movsesijan, Alisa
AU  - Ilić, Nataša
AU  - Vasilev, Saša
AU  - Dinić, Miroslav
AU  - Golić, Nataša
AU  - Vučević, Dragana
AU  - Čolić, Miodrag
AU  - Tomić, Sergej
AU  - Đokić, Jelena
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1875
AB  - The role of gut microbiota composition in efficacy of various immune-based therapies is increasingly recognized.
Thus, the aim of our study was to investigate if the efficacy of myeloid-derived suppressor cells
(MDSC)-Prostaglandin E2 (PGE2) therapy for multiple sclerosis (MS) correlates with gut microbiota composition
and function. MDSC generated from bone marrow cells in the presence of PGE2 were applied to spinal
cord homogenate/CFA-induced experimental autoimmune encephalomyelitis (EAE) in Dark Agouti (DA)
rats, an animal model of MS. MDSC-PGE2 therapy resulted in a significant attenuation of EAE symptoms
over 30 days of disease monitoring. These results correlated with lower percentage of proinflammatory interferon-
gamma and interleukin-17 producing cells and higher percentage of anti-inflammatory IL-4 producing
cells in spinal cord and spleen. Gut microbial composition were studied using amplicon(16S rRNA)-based
metagenomic analyses of fecal samples collected prior to the induction of EAE and MDSC-PGE2 therapy application,
and at the peak of the disease. The induction of EAE resulted in a decrease of microbiota diversity,
whereas the MDSC-PGE2 therapy preserved the diversity in EAE-induced animals. The induction of EAE
in control group associated with a higher relative abundance of Peptococcaceae, but the lower levels of Veillonellaceae
and different groups of Prevotellaceae, known to produce immunosuppressive short chain fatty
acid (SCFA), and Lactobacillus reuteri, known for its anti-inflammatory function. In contrast, there were no
changes in levels of these immunoregulatory taxa in EAE-animals treated with MDSC-PGE2 therapy. Also,
SCFA producing Ruminococcaceae, and Coriobacteriaceae, known to metabolize phytoestrogens to immunosuppressive
metabolites were more abundant in EAE-animals treated with MDSC-PGE2 therapy. Predicted
metabolic profiling obtained by PICRUSt2 revealed that pathways involved in biosynthesis of polyamines,
metabolites known to contribute to homeostasis of gastrointestinal mucosa, were enriched in MDSC-PGE2
treated animals. Considering these results, the modification of gut microbiota composition and function
could further increase efficacy of MDSC-PGE-2 based therapy of autoimmune diseases.
PB  - Novi Sad : Faculty of Sciences, Department of Biology and Ecology
C3  - Biologia Serbica
T1  - Myeloid derived suppressor cells-therapy attenuates experimental autoimmune encephalomyelitis and modulates gut microbiota composition
IS  - 1 (Special Edition)
SP  - 98
VL  - 43
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1875
ER  - 

@conference{
author = "Radojević, Dušan and Bekić, Marina and Gruden-Movsesijan, Alisa and Ilić, Nataša and Vasilev, Saša and Dinić, Miroslav and Golić, Nataša and Vučević, Dragana and Čolić, Miodrag and Tomić, Sergej and Đokić, Jelena",
year = "2021",
abstract = "The role of gut microbiota composition in efficacy of various immune-based therapies is increasingly recognized.
Thus, the aim of our study was to investigate if the efficacy of myeloid-derived suppressor cells
(MDSC)-Prostaglandin E2 (PGE2) therapy for multiple sclerosis (MS) correlates with gut microbiota composition
and function. MDSC generated from bone marrow cells in the presence of PGE2 were applied to spinal
cord homogenate/CFA-induced experimental autoimmune encephalomyelitis (EAE) in Dark Agouti (DA)
rats, an animal model of MS. MDSC-PGE2 therapy resulted in a significant attenuation of EAE symptoms
over 30 days of disease monitoring. These results correlated with lower percentage of proinflammatory interferon-
gamma and interleukin-17 producing cells and higher percentage of anti-inflammatory IL-4 producing
cells in spinal cord and spleen. Gut microbial composition were studied using amplicon(16S rRNA)-based
metagenomic analyses of fecal samples collected prior to the induction of EAE and MDSC-PGE2 therapy application,
and at the peak of the disease. The induction of EAE resulted in a decrease of microbiota diversity,
whereas the MDSC-PGE2 therapy preserved the diversity in EAE-induced animals. The induction of EAE
in control group associated with a higher relative abundance of Peptococcaceae, but the lower levels of Veillonellaceae
and different groups of Prevotellaceae, known to produce immunosuppressive short chain fatty
acid (SCFA), and Lactobacillus reuteri, known for its anti-inflammatory function. In contrast, there were no
changes in levels of these immunoregulatory taxa in EAE-animals treated with MDSC-PGE2 therapy. Also,
SCFA producing Ruminococcaceae, and Coriobacteriaceae, known to metabolize phytoestrogens to immunosuppressive
metabolites were more abundant in EAE-animals treated with MDSC-PGE2 therapy. Predicted
metabolic profiling obtained by PICRUSt2 revealed that pathways involved in biosynthesis of polyamines,
metabolites known to contribute to homeostasis of gastrointestinal mucosa, were enriched in MDSC-PGE2
treated animals. Considering these results, the modification of gut microbiota composition and function
could further increase efficacy of MDSC-PGE-2 based therapy of autoimmune diseases.",
publisher = "Novi Sad : Faculty of Sciences, Department of Biology and Ecology",
journal = "Biologia Serbica",
title = "Myeloid derived suppressor cells-therapy attenuates experimental autoimmune encephalomyelitis and modulates gut microbiota composition",
number = "1 (Special Edition)",
pages = "98",
volume = "43",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1875"
}

Radojević, D., Bekić, M., Gruden-Movsesijan, A., Ilić, N., Vasilev, S., Dinić, M., Golić, N., Vučević, D., Čolić, M., Tomić, S.,& Đokić, J.. (2021). Myeloid derived suppressor cells-therapy attenuates experimental autoimmune encephalomyelitis and modulates gut microbiota composition. in Biologia Serbica
Novi Sad : Faculty of Sciences, Department of Biology and Ecology., 43(1 (Special Edition)), 98.
https://hdl.handle.net/21.15107/rcub_imagine_1875

Radojević D, Bekić M, Gruden-Movsesijan A, Ilić N, Vasilev S, Dinić M, Golić N, Vučević D, Čolić M, Tomić S, Đokić J. Myeloid derived suppressor cells-therapy attenuates experimental autoimmune encephalomyelitis and modulates gut microbiota composition. in Biologia Serbica. 2021;43(1 (Special Edition)):98.
https://hdl.handle.net/21.15107/rcub_imagine_1875 .

Radojević, Dušan, Bekić, Marina, Gruden-Movsesijan, Alisa, Ilić, Nataša, Vasilev, Saša, Dinić, Miroslav, Golić, Nataša, Vučević, Dragana, Čolić, Miodrag, Tomić, Sergej, Đokić, Jelena, "Myeloid derived suppressor cells-therapy attenuates experimental autoimmune encephalomyelitis and modulates gut microbiota composition" in Biologia Serbica, 43, no. 1 (Special Edition) (2021):98,
https://hdl.handle.net/21.15107/rcub_imagine_1875 .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Jakovljević, Stefan
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Radojević, Dušan
AU  - Strahinić, Ivana
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1473
AB  - The host-microbiota cross-talk represents an important factor contributing to innate immune response and host resistance during infection. It has been shown that probiotic lactobacilli exhibit the ability to modulate innate immunity and enhance pathogen elimination. Here we showed that heat-inactivated probiotic strain Lactobacillus curvatus BGMK2-41 stimulates immune response and resistance of the Caenorhabditis elegans against Staphylococcus aureus and Pseudomonas aeruginosa. By employing qRT-PCR and western blot analysis we showed that heat-inactivated BGMK2-41 activated PMK-1/p38 MAPK immunity pathway which prolongs the survival of C. elegans exposed to pathogenic bacteria in nematode killing assays. The C. elegans pmk-1 mutant was used to demonstrate a mechanistic basis for the antimicrobial potential of BGMK2-41, showing that BGMK2-41 upregulated PMK-1/p38 MAPK dependent transcription of C-type lectins, lysozymes and tight junction protein CLC-1. Overall, this study suggests that PMK-1/p38 MAPK-dependent immune regulation by BGMK2-41 is essential for probiotic-mediated C. elegans protection against gram-positive and gram-negative bacteria and could be further explored for development of probiotics with the potential to increase resistance of the host towards pathogens.
PB  - Nature Portfolio, Berlin
T2  - Scientific Reports
T1  - Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-00698-5
ER  - 

@article{
author = "Dinić, Miroslav and Jakovljević, Stefan and Đokić, Jelena and Popović, Nikola and Radojević, Dušan and Strahinić, Ivana and Golić, Nataša",
year = "2021",
abstract = "The host-microbiota cross-talk represents an important factor contributing to innate immune response and host resistance during infection. It has been shown that probiotic lactobacilli exhibit the ability to modulate innate immunity and enhance pathogen elimination. Here we showed that heat-inactivated probiotic strain Lactobacillus curvatus BGMK2-41 stimulates immune response and resistance of the Caenorhabditis elegans against Staphylococcus aureus and Pseudomonas aeruginosa. By employing qRT-PCR and western blot analysis we showed that heat-inactivated BGMK2-41 activated PMK-1/p38 MAPK immunity pathway which prolongs the survival of C. elegans exposed to pathogenic bacteria in nematode killing assays. The C. elegans pmk-1 mutant was used to demonstrate a mechanistic basis for the antimicrobial potential of BGMK2-41, showing that BGMK2-41 upregulated PMK-1/p38 MAPK dependent transcription of C-type lectins, lysozymes and tight junction protein CLC-1. Overall, this study suggests that PMK-1/p38 MAPK-dependent immune regulation by BGMK2-41 is essential for probiotic-mediated C. elegans protection against gram-positive and gram-negative bacteria and could be further explored for development of probiotics with the potential to increase resistance of the host towards pathogens.",
publisher = "Nature Portfolio, Berlin",
journal = "Scientific Reports",
title = "Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-00698-5"
}

Dinić, M., Jakovljević, S., Đokić, J., Popović, N., Radojević, D., Strahinić, I.,& Golić, N.. (2021). Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria. in Scientific Reports
Nature Portfolio, Berlin., 11(1).
https://doi.org/10.1038/s41598-021-00698-5

Dinić M, Jakovljević S, Đokić J, Popović N, Radojević D, Strahinić I, Golić N. Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-00698-5 .

Dinić, Miroslav, Jakovljević, Stefan, Đokić, Jelena, Popović, Nikola, Radojević, Dušan, Strahinić, Ivana, Golić, Nataša, "Probiotic-mediated p38 MAPK immune signaling prolongs the survival of Caenorhabditis elegans exposed to pathogenic bacteria" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-00698-5 . .

TY  - JOUR
AU  - Tomić, Sergej
AU  - Đokić, Jelena
AU  - Stevanović, Dejan
AU  - Ilić, Nataša
AU  - Gruden-Movsesijan, Alisa
AU  - Dinić, Miroslav
AU  - Radojević, Dušan
AU  - Bekić, Marina
AU  - Mitrović, Nebojša
AU  - Tomasević, Ratko
AU  - Mikić, Dragan
AU  - Stojanović, Dragos
AU  - Čolić, Miodrag
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1446
AB  - Widespread coronavirus disease (COVID)-19 is causing pneumonia, respiratory and multiorgan failure in susceptible individuals. Dysregulated immune response marks severe COVID-19, but the immunological mechanisms driving COVID-19 pathogenesis are still largely unknown, which is hampering the development of efficient treatments. Here we analyzed similar to 140 parameters of cellular and humoral immune response in peripheral blood of 41 COVID-19 patients and 16 age/gender-matched healthy donors by flow-cytometry, quantitative PCR, western blot and ELISA, followed by integrated correlation analyses with similar to 30 common clinical and laboratory parameters. We found that lymphocytopenia in severe COVID-19 patients (n=20) strongly affects T, NK and NKT cells, but not B cells and antibody production. Unlike increased activation of ICOS-1+ CD4+ T cells in mild COVID-19 patients (n=21), T cells in severe patients showed impaired activation, low IFN-gamma production and high functional exhaustion, which correlated with significantly down-regulated HLA-DR expression in monocytes, dendritic cells and B cells. The latter phenomenon was followed by lower interferon responsive factor (IRF)-8 and autophagy-related genes expressions, and the expansion of myeloid derived suppressor cells (MDSC). Intriguingly, PD-L1-, ILT-3-, and IDO-1-expressing monocytic MDSC were the dominant producers of IL-6 and IL-10, which correlated with the increased inflammation and accumulation of regulatory B and T cell subsets in severe COVID-19 patients. Overall, down-regulated IRF-8 and autophagy-related genes expression, and the expansion of MDSC subsets could play critical roles in dysregulating T cell response in COVID-19, which could have large implications in diagnostics and design of novel therapeutics for this disease.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Immunology
T1  - Reduced Expression of Autophagy Markers and Expansion of Myeloid-Derived Suppressor Cells Correlate With Poor T Cell Response in Severe COVID-19 Patients
VL  - 12
DO  - 10.3389/fimmu.2021.614599
ER  - 

@article{
author = "Tomić, Sergej and Đokić, Jelena and Stevanović, Dejan and Ilić, Nataša and Gruden-Movsesijan, Alisa and Dinić, Miroslav and Radojević, Dušan and Bekić, Marina and Mitrović, Nebojša and Tomasević, Ratko and Mikić, Dragan and Stojanović, Dragos and Čolić, Miodrag",
year = "2021",
abstract = "Widespread coronavirus disease (COVID)-19 is causing pneumonia, respiratory and multiorgan failure in susceptible individuals. Dysregulated immune response marks severe COVID-19, but the immunological mechanisms driving COVID-19 pathogenesis are still largely unknown, which is hampering the development of efficient treatments. Here we analyzed similar to 140 parameters of cellular and humoral immune response in peripheral blood of 41 COVID-19 patients and 16 age/gender-matched healthy donors by flow-cytometry, quantitative PCR, western blot and ELISA, followed by integrated correlation analyses with similar to 30 common clinical and laboratory parameters. We found that lymphocytopenia in severe COVID-19 patients (n=20) strongly affects T, NK and NKT cells, but not B cells and antibody production. Unlike increased activation of ICOS-1+ CD4+ T cells in mild COVID-19 patients (n=21), T cells in severe patients showed impaired activation, low IFN-gamma production and high functional exhaustion, which correlated with significantly down-regulated HLA-DR expression in monocytes, dendritic cells and B cells. The latter phenomenon was followed by lower interferon responsive factor (IRF)-8 and autophagy-related genes expressions, and the expansion of myeloid derived suppressor cells (MDSC). Intriguingly, PD-L1-, ILT-3-, and IDO-1-expressing monocytic MDSC were the dominant producers of IL-6 and IL-10, which correlated with the increased inflammation and accumulation of regulatory B and T cell subsets in severe COVID-19 patients. Overall, down-regulated IRF-8 and autophagy-related genes expression, and the expansion of MDSC subsets could play critical roles in dysregulating T cell response in COVID-19, which could have large implications in diagnostics and design of novel therapeutics for this disease.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Immunology",
title = "Reduced Expression of Autophagy Markers and Expansion of Myeloid-Derived Suppressor Cells Correlate With Poor T Cell Response in Severe COVID-19 Patients",
volume = "12",
doi = "10.3389/fimmu.2021.614599"
}

Tomić, S., Đokić, J., Stevanović, D., Ilić, N., Gruden-Movsesijan, A., Dinić, M., Radojević, D., Bekić, M., Mitrović, N., Tomasević, R., Mikić, D., Stojanović, D.,& Čolić, M.. (2021). Reduced Expression of Autophagy Markers and Expansion of Myeloid-Derived Suppressor Cells Correlate With Poor T Cell Response in Severe COVID-19 Patients. in Frontiers in Immunology
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fimmu.2021.614599

Tomić S, Đokić J, Stevanović D, Ilić N, Gruden-Movsesijan A, Dinić M, Radojević D, Bekić M, Mitrović N, Tomasević R, Mikić D, Stojanović D, Čolić M. Reduced Expression of Autophagy Markers and Expansion of Myeloid-Derived Suppressor Cells Correlate With Poor T Cell Response in Severe COVID-19 Patients. in Frontiers in Immunology. 2021;12.
doi:10.3389/fimmu.2021.614599 .

Tomić, Sergej, Đokić, Jelena, Stevanović, Dejan, Ilić, Nataša, Gruden-Movsesijan, Alisa, Dinić, Miroslav, Radojević, Dušan, Bekić, Marina, Mitrović, Nebojša, Tomasević, Ratko, Mikić, Dragan, Stojanović, Dragos, Čolić, Miodrag, "Reduced Expression of Autophagy Markers and Expansion of Myeloid-Derived Suppressor Cells Correlate With Poor T Cell Response in Severe COVID-19 Patients" in Frontiers in Immunology, 12 (2021),
https://doi.org/10.3389/fimmu.2021.614599 . .

TY  - JOUR
AU  - Klionsky, Daniel J.
AU  - Abdel-Aziz, Amal Kamal
AU  - Dini, Luciana
AU  - Dinić, Miroslav
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1422
AB  - In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Autophagy
T1  - Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
EP  - 382
IS  - 1
SP  - 1
VL  - 17
DO  - 10.1080/15548627.2020.1797280
ER  - 

@article{
author = "Klionsky, Daniel J. and Abdel-Aziz, Amal Kamal and Dini, Luciana and Dinić, Miroslav",
year = "2021",
abstract = "In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Autophagy",
title = "Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)",
pages = "382-1",
number = "1",
volume = "17",
doi = "10.1080/15548627.2020.1797280"
}

Klionsky, D. J., Abdel-Aziz, A. K., Dini, L.,& Dinić, M.. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). in Autophagy
Taylor & Francis Inc, Philadelphia., 17(1), 1-382.
https://doi.org/10.1080/15548627.2020.1797280

Klionsky DJ, Abdel-Aziz AK, Dini L, Dinić M. Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). in Autophagy. 2021;17(1):1-382.
doi:10.1080/15548627.2020.1797280 .

Klionsky, Daniel J., Abdel-Aziz, Amal Kamal, Dini, Luciana, Dinić, Miroslav, "Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)" in Autophagy, 17, no. 1 (2021):1-382,
https://doi.org/10.1080/15548627.2020.1797280 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Herholz, Marija
AU  - Kacarević, Uros
AU  - Radojević, Dušan
AU  - Novović, Katarina
AU  - Đokić, Jelena
AU  - Trifunović, Aleksandra
AU  - Golić, Nataša
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1431
AB  - Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host.
PB  - Impact Journals LLC
T2  - Aging
T1  - Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy
EP  - 8054
IS  - 6
SP  - 8040
VL  - 13
DO  - 10.18632/aging.202885
ER  - 

@article{
author = "Dinić, Miroslav and Herholz, Marija and Kacarević, Uros and Radojević, Dušan and Novović, Katarina and Đokić, Jelena and Trifunović, Aleksandra and Golić, Nataša",
year = "2021",
abstract = "Gut homeostasis is maintained by the close interaction between commensal intestinal microbiota and the host, affecting the most complex physiological processes, such as aging. Some commensal bacteria with the potential to promote healthy aging arise as attractive candidates for the development of pro-longevity probiotics. Here, we showed that heat-inactivated human commensal Lactobacillus fermentum BGHV110 (BGHV110) extends the lifespan of Caenorhabditis elegans and improves age-related physiological features, including locomotor function and lipid metabolism. Mechanistically, we found that BGHV110 promotes HLH30/TFEB-dependent autophagy to delay aging, as longevity assurance was completely abolished in the mutant lacking HLH-30, a major autophagy regulator in C. elegans. Moreover, we observed that BGHV110 partially decreased the content of lipid droplets in an HLH-30-dependent manner and, at the same time, slightly increased mitochondrial activity. In summary, this study demonstrates that specific factors from commensal bacteria can be used to exploit HLH-30/TFEB-mediated autophagy in order to promote longevity and fitness of the host.",
publisher = "Impact Journals LLC",
journal = "Aging",
title = "Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy",
pages = "8054-8040",
number = "6",
volume = "13",
doi = "10.18632/aging.202885"
}

Dinić, M., Herholz, M., Kacarević, U., Radojević, D., Novović, K., Đokić, J., Trifunović, A.,& Golić, N.. (2021). Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy. in Aging
Impact Journals LLC., 13(6), 8040-8054.
https://doi.org/10.18632/aging.202885

Dinić M, Herholz M, Kacarević U, Radojević D, Novović K, Đokić J, Trifunović A, Golić N. Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy. in Aging. 2021;13(6):8040-8054.
doi:10.18632/aging.202885 .

Dinić, Miroslav, Herholz, Marija, Kacarević, Uros, Radojević, Dušan, Novović, Katarina, Đokić, Jelena, Trifunović, Aleksandra, Golić, Nataša, "Host-commensal interaction promotes health and lifespan in Caenorhabditis elegans through the activation of HLH-30/TFEB-mediated autophagy" in Aging, 13, no. 6 (2021):8040-8054,
https://doi.org/10.18632/aging.202885 . .

TY  - JOUR
AU  - Brdarić, Emilija
AU  - Soković Bajić, Svetlana
AU  - Đokić, Jelena
AU  - Durdić, Sladana
AU  - Ruas-Madiedo, Patricia
AU  - Stevanović, Magdalena
AU  - Tolinački, Maja
AU  - Dinić, Miroslav
AU  - Mutić, Jelena
AU  - Golić, Nataša
AU  - Živković, Milica
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1471
AB  - Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells
VL  - 12
DO  - 10.3389/fmicb.2021.759378
ER  - 

@article{
author = "Brdarić, Emilija and Soković Bajić, Svetlana and Đokić, Jelena and Durdić, Sladana and Ruas-Madiedo, Patricia and Stevanović, Magdalena and Tolinački, Maja and Dinić, Miroslav and Mutić, Jelena and Golić, Nataša and Živković, Milica",
year = "2021",
abstract = "Cadmium (Cd) ranks seventh on the list of most significant potential threats to human health based on its suspected toxicity and the possibility of exposure to it. It has been reported that some bacterial exopolysaccharides (EPSs) have the ability to bind heavy metal ions. We therefore investigated the capacity of eight EPS-producing lactobacilli to adsorb Cd in the present study, and Lactiplantibacillus plantarum BGAN8 was chosen as the best candidate. In addition, we demonstrate that an EPS derived from BGAN8 (EPS-AN8) exhibits a high Cd-binding capacity and prevents Cd-mediated toxicity in intestinal epithelial Caco-2 cells. Simultaneous use of EPS-AN8 with Cd treatment prevents inflammation, disruption of tight-junction proteins, and oxidative stress. Our results indicate that the EPS in question has a strong potential to be used as a postbiotic in combatting the adverse effects of Cd. Moreover, we show that higher concentrations of EPS-AN8 can alleviate Cd-induced cell damage.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells",
volume = "12",
doi = "10.3389/fmicb.2021.759378"
}

Brdarić, E., Soković Bajić, S., Đokić, J., Durdić, S., Ruas-Madiedo, P., Stevanović, M., Tolinački, M., Dinić, M., Mutić, J., Golić, N.,& Živković, M.. (2021). Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fmicb.2021.759378

Brdarić E, Soković Bajić S, Đokić J, Durdić S, Ruas-Madiedo P, Stevanović M, Tolinački M, Dinić M, Mutić J, Golić N, Živković M. Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells. in Frontiers in Microbiology. 2021;12.
doi:10.3389/fmicb.2021.759378 .

Brdarić, Emilija, Soković Bajić, Svetlana, Đokić, Jelena, Durdić, Sladana, Ruas-Madiedo, Patricia, Stevanović, Magdalena, Tolinački, Maja, Dinić, Miroslav, Mutić, Jelena, Golić, Nataša, Živković, Milica, "Protective Effect of an Exopolysaccharide Produced by Lactiplantibacillus plantarum BGAN8 Against Cadmium-Induced Toxicity in Caco-2 Cells" in Frontiers in Microbiology, 12 (2021),
https://doi.org/10.3389/fmicb.2021.759378 . .

TY  - CONF
AU  - Bekić, Marina
AU  - Dinić, Miroslav
AU  - Radojević, Dušan
AU  - Ilić, Nataša
AU  - Vucević, Dragana
AU  - Vasilev, Sasa
AU  - Đokić, Jelena
AU  - Tomić, Sergej
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1436
PB  - Wiley, Hoboken
C3  - European Journal of Immunology
T1  - Prostaglandin E2 differentially regulate the suppressive mechanisms in myeloid derived suppressor cells subsets in vitro
EP  - 179
SP  - 179
VL  - 51
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1436
ER  - 

@conference{
author = "Bekić, Marina and Dinić, Miroslav and Radojević, Dušan and Ilić, Nataša and Vucević, Dragana and Vasilev, Sasa and Đokić, Jelena and Tomić, Sergej",
year = "2021",
publisher = "Wiley, Hoboken",
journal = "European Journal of Immunology",
title = "Prostaglandin E2 differentially regulate the suppressive mechanisms in myeloid derived suppressor cells subsets in vitro",
pages = "179-179",
volume = "51",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1436"
}

Bekić, M., Dinić, M., Radojević, D., Ilić, N., Vucević, D., Vasilev, S., Đokić, J.,& Tomić, S.. (2021). Prostaglandin E2 differentially regulate the suppressive mechanisms in myeloid derived suppressor cells subsets in vitro. in European Journal of Immunology
Wiley, Hoboken., 51, 179-179.
https://hdl.handle.net/21.15107/rcub_imagine_1436

Bekić M, Dinić M, Radojević D, Ilić N, Vucević D, Vasilev S, Đokić J, Tomić S. Prostaglandin E2 differentially regulate the suppressive mechanisms in myeloid derived suppressor cells subsets in vitro. in European Journal of Immunology. 2021;51:179-179.
https://hdl.handle.net/21.15107/rcub_imagine_1436 .

Bekić, Marina, Dinić, Miroslav, Radojević, Dušan, Ilić, Nataša, Vucević, Dragana, Vasilev, Sasa, Đokić, Jelena, Tomić, Sergej, "Prostaglandin E2 differentially regulate the suppressive mechanisms in myeloid derived suppressor cells subsets in vitro" in European Journal of Immunology, 51 (2021):179-179,
https://hdl.handle.net/21.15107/rcub_imagine_1436 .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina T.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1509
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2 '-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley-V C H Verlag Gmbh, Weinheim
T2  - Chemistry & Biodiversity
T1  - Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression
IS  - 1
VL  - 18
DO  - 10.1002/cbdv.202000786
ER  - 

@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina T.",
year = "2021",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2 '-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley-V C H Verlag Gmbh, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression",
number = "1",
volume = "18",
doi = "10.1002/cbdv.202000786"
}

Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M. T.. (2021). Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity
Wiley-V C H Verlag Gmbh, Weinheim., 18(1).
https://doi.org/10.1002/cbdv.202000786

Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković MT. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression. in Chemistry & Biodiversity. 2021;18(1).
doi:10.1002/cbdv.202000786 .

Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina T., "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expression" in Chemistry & Biodiversity, 18, no. 1 (2021),
https://doi.org/10.1002/cbdv.202000786 . .