TY  - CONF
AU  - Kojić, Snežana
AU  - Bošković, Srđan
AU  - Milovanović, Mina
AU  - Stainie, Didier
AU  - Juez, Rubén Marín
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
PY  - 2024
UR  - https://www.izfs.org/education/10sczi
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2309
AB  - In contrast to humans, zebrafish have a remarkable ability to regenerate their hearts after injury, while both humans and zebrafish efficiently repair the wounded skeletal muscle. Common players in these two processes might represent potential targets for the development of efficient therapies to stimulate human heart to regenerate after injury. We identified ankrd1a expression to be upregulated in both regenerating zebrafish hearts and in repairing skeletal muscle. Its mammalian homolog ANKRD1/CARP encodes a stress responsive cardiac ankyrin repeat protein involved in transcriptional regulation, sarcomere assembly and mechanosensing. Using a TgBAC(ankrd1a:EGFP) line, we showed that activation of ankrd1a in cryoinjured heart is restricted to border zone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of regenerating cardiomyocytes. After stab wound injury of skeletal muscle expression of the fluorescent reporter was observed from 3 dpi, when new EGFP-positive muscle cells emerged inside the injury zone. At later time points, EGFP-positive myofibers were visible in the deeper tissue layers, concomitant with active repair of the injured tissue. In cryoinjured skeletal muscle, strong activation of ankrd1a was also observed in myofibers adjacent to the injury, and in those on uninjured side. Detection of the transgene in both newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury suggests the role of ankrd1a in skeletal muscle tissue repair and adaptive processes in uninjured myofibers surrounding the injury site. Our results implicate ankrd1a in zebrafish muscle regeneration, repair and remodeling, promoting it as an attractive target for translational studies, as a player in muscle healing and as a sensor of stressed muscle.
PB  - Society for Zebrafish Research
C3  - 10th Strategic Conference of Zebrafish Investigators
T1  - Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2309
ER  - 

@conference{
author = "Kojić, Snežana and Bošković, Srđan and Milovanović, Mina and Stainie, Didier and Juez, Rubén Marín and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija",
year = "2024",
abstract = "In contrast to humans, zebrafish have a remarkable ability to regenerate their hearts after injury, while both humans and zebrafish efficiently repair the wounded skeletal muscle. Common players in these two processes might represent potential targets for the development of efficient therapies to stimulate human heart to regenerate after injury. We identified ankrd1a expression to be upregulated in both regenerating zebrafish hearts and in repairing skeletal muscle. Its mammalian homolog ANKRD1/CARP encodes a stress responsive cardiac ankyrin repeat protein involved in transcriptional regulation, sarcomere assembly and mechanosensing. Using a TgBAC(ankrd1a:EGFP) line, we showed that activation of ankrd1a in cryoinjured heart is restricted to border zone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of regenerating cardiomyocytes. After stab wound injury of skeletal muscle expression of the fluorescent reporter was observed from 3 dpi, when new EGFP-positive muscle cells emerged inside the injury zone. At later time points, EGFP-positive myofibers were visible in the deeper tissue layers, concomitant with active repair of the injured tissue. In cryoinjured skeletal muscle, strong activation of ankrd1a was also observed in myofibers adjacent to the injury, and in those on uninjured side. Detection of the transgene in both newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury suggests the role of ankrd1a in skeletal muscle tissue repair and adaptive processes in uninjured myofibers surrounding the injury site. Our results implicate ankrd1a in zebrafish muscle regeneration, repair and remodeling, promoting it as an attractive target for translational studies, as a player in muscle healing and as a sensor of stressed muscle.",
publisher = "Society for Zebrafish Research",
journal = "10th Strategic Conference of Zebrafish Investigators",
title = "Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2309"
}

Kojić, S., Bošković, S., Milovanović, M., Stainie, D., Juez, R. M., Jasnić, J., Novković, M.,& Milošević, E.. (2024). Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair. in 10th Strategic Conference of Zebrafish Investigators
Society for Zebrafish Research..
https://hdl.handle.net/21.15107/rcub_imagine_2309

Kojić S, Bošković S, Milovanović M, Stainie D, Juez RM, Jasnić J, Novković M, Milošević E. Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair. in 10th Strategic Conference of Zebrafish Investigators. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2309 .

Kojić, Snežana, Bošković, Srđan, Milovanović, Mina, Stainie, Didier, Juez, Rubén Marín, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, "Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair" in 10th Strategic Conference of Zebrafish Investigators (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2309 .

TY  - CONF
AU  - Milovanović, Mina
AU  - Bošković, Srđan
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Kojić, Snežana
PY  - 2023
UR  - https://zebrafish2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2023
AB  - In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation
of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close
proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle
regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and
remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal
expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections
prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury
(dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi.
At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry,
while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the
deeper tissue layers within the injury, indicating active repair of the injured tissue. To identify cells
in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells,
undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was
detected both in the newly formed myofibers that invade the wound and in the apparently uninjured
tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged
myofibers surrounding the physical injury.
C3  - 12th European Zebrafish Meeting
T1  - Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle
EP  - 276
SP  - 276
SP  - 0254
VL  - 14
VL  - 12
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2023
ER  - 

@conference{
author = "Milovanović, Mina and Bošković, Srđan and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Kojić, Snežana",
year = "2023",
abstract = "In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation
of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close
proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle
regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and
remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal
expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections
prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury
(dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi.
At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry,
while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the
deeper tissue layers within the injury, indicating active repair of the injured tissue. To identify cells
in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells,
undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was
detected both in the newly formed myofibers that invade the wound and in the apparently uninjured
tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged
myofibers surrounding the physical injury.",
journal = "12th European Zebrafish Meeting",
title = "Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle",
pages = "276-276-0254",
volume = "14, 12",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2023"
}

Milovanović, M., Bošković, S., Jasnić, J., Novković, M., Milošević, E.,& Kojić, S.. (2023). Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle. in 12th European Zebrafish Meeting, 14, 276-276.
https://hdl.handle.net/21.15107/rcub_imagine_2023

Milovanović M, Bošković S, Jasnić J, Novković M, Milošević E, Kojić S. Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle. in 12th European Zebrafish Meeting. 2023;14:276-276.
https://hdl.handle.net/21.15107/rcub_imagine_2023 .

Milovanović, Mina, Bošković, Srđan, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Kojić, Snežana, "Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle" in 12th European Zebrafish Meeting, 14 (2023):276-276,
https://hdl.handle.net/21.15107/rcub_imagine_2023 .

TY  - JOUR
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1928
AB  - Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.
PB  - Springer Nature
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines
DO  - 10.1007/s00432-023-04930-9
ER  - 

@article{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
abstract = "Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.",
publisher = "Springer Nature",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines",
doi = "10.1007/s00432-023-04930-9"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology
Springer Nature..
https://doi.org/10.1007/s00432-023-04930-9

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. 2023;.
doi:10.1007/s00432-023-04930-9 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines" in Journal of Cancer Research and Clinical Oncology (2023),
https://doi.org/10.1007/s00432-023-04930-9 . .

TY  - CONF
AU  - Milovanović, Mina
AU  - Bošković, Srđan
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Kojić, Snežana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2132
AB  - Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart
through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of
new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary
vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration.
Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing
ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and
7 days. Dedifferentiating cardiomyocytes and epicardial cells were immunostained with anti-MYH7 and
anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy.
Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between
the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7.
The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating
zebrafish heart.
Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone
cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute
to the regeneration process occuring in these layers of the heart.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Spatial profile of ankrd1a activation during regeneration of zebrafish heart
EP  - 141
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2132
ER  - 

@conference{
author = "Milovanović, Mina and Bošković, Srđan and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Kojić, Snežana",
year = "2023",
abstract = "Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart
through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of
new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary
vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration.
Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing
ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and
7 days. Dedifferentiating cardiomyocytes and epicardial cells were immunostained with anti-MYH7 and
anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy.
Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between
the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7.
The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating
zebrafish heart.
Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone
cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute
to the regeneration process occuring in these layers of the heart.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Spatial profile of ankrd1a activation during regeneration of zebrafish heart",
pages = "141-141",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2132"
}

Milovanović, M., Bošković, S., Jasnić, J., Novković, M., Milošević, E.,& Kojić, S.. (2023). Spatial profile of ankrd1a activation during regeneration of zebrafish heart. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 141-141.
https://hdl.handle.net/21.15107/rcub_imagine_2132

Milovanović M, Bošković S, Jasnić J, Novković M, Milošević E, Kojić S. Spatial profile of ankrd1a activation during regeneration of zebrafish heart. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:141-141.
https://hdl.handle.net/21.15107/rcub_imagine_2132 .

Milovanović, Mina, Bošković, Srđan, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Kojić, Snežana, "Spatial profile of ankrd1a activation during regeneration of zebrafish heart" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):141-141,
https://hdl.handle.net/21.15107/rcub_imagine_2132 .

TY  - JOUR
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1918
AB  - Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.
PB  - Springer Nature
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines
DO  - 10.1007/s00432-023-04930-9
ER  - 

@article{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
abstract = "Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.",
publisher = "Springer Nature",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines",
doi = "10.1007/s00432-023-04930-9"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology
Springer Nature..
https://doi.org/10.1007/s00432-023-04930-9

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. 2023;.
doi:10.1007/s00432-023-04930-9 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines" in Journal of Cancer Research and Clinical Oncology (2023),
https://doi.org/10.1007/s00432-023-04930-9 . .

TY  - DATA
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1929
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1929
ER  - 

@misc{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1929"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9. in Journal of Cancer Research and Clinical Oncology.
https://hdl.handle.net/21.15107/rcub_imagine_1929

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9. in Journal of Cancer Research and Clinical Oncology. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1929 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9" in Journal of Cancer Research and Clinical Oncology (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1929 .

TY  - CONF
AU  - Kojić, Snežana
AU  - Bošković, Srđan
AU  - Milovanović, Mina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1746
AB  - Nakon infarkta miokarda povređeno srce čoveka ne može da se regeneriše, već
reaguje formiranjem fibrotičnog ožiljka i remodelovanjem miokarda, koji dovode
do slabljenja njegove funkcije. Miokard sisara je dugo vremena smatran
postmitotičkim i terminalno diferenciranim tkivom. Međutim, kardiomiociti
sisara poseduju ograničenu sposobnost proliferacije. Njihova deoba je redak
događaj i odvija se po veoma niskoj stopi, što svakako nije dovoljno da nadoknadi
milione kardiomiocita trajno izgubljenih usled infarkta miokarda. Trenutno se
u svetu razvijaju dve strategije za podsticanje regeneracije povređenog srca
čoveka kako bi se povratile njegova struktura i funkcija. Jedna strategija
podrazumeva naseljavanje oštećenog tkiva zdravim kardiomiocitima poreklom od
indukovanih pluripotentnih ćelija, dok je cilj druge strategije aktivacija
endogenih mehanizama regeneracije. Za razliku od čoveka, neki kičmenjaci imaju
sposobnost regeneracije povređenih organa, uključujući srce. Životinja koja se
najčešće koristi za proučavanje regeneracije srca je riba zebrica (Danio rerio).
Pionirska studija o izuzetnom regenerativnom kapacitetu srca zebrice nakon
amputacije dela komore objavljena je 2002. godine, nakon koje je usledilo mnoštvo
publikacija o ćelijskim i molekularnim mehanizmima koji doprinose
regenerativnom odgovoru. Regeneracija srca zebrice je rezultat strogo regulisane
interakcije većeg broja procesa, uključujući inflamatorni odgovor,
dediferencijaciju i proliferaciju kardiomiocita, neovaskularizaciju i
reorganizaciju ekstraćelijskog matriksa. Regeneracija se može posmatrati kao
uspavan proces u organima koji ne regenerišu i manipulacijom ovog procesa bi
se mogla postići reaktivacija proliferacije u tim organima nakon povrede.
Ispitivanje interakcije između pro-regenerišućih mehanizama i procesa koji
utiču na regenerativni kapacitet treba da dovede do identifikacije faktora
potrebnih za prevazilaženje blokade regeneracije. Na taj način bi se razvile nove
strategije za indukciju proliferacije kardiomiocita i regeneraciju srca čoveka.
AB  - Након инфаркта миокарда повређено срце човека не може да се регенерише, већ
реагује формирањем фибротичног ожиљка и ремоделовањем миокарда, који доводе
до слабљења његове функције. Миокард сисара је дуго времена сматран
постмитотичким и терминално диференцираним ткивом. Међутим, кардиомиоцити
сисара поседују ограничену способност пролиферације. Њихова деоба је редак
догађај и одвија се по веома ниској стопи, што свакако није довољно да надокнади
милионе кардиомиоцита трајно изгубљених услед инфаркта миокарда. Тренутно се
у свету развијају две стратегије за подстицање регенерације повређеног срца
човека како би се повратиле његова структура и функција. Једна стратегија
подразумева насељавање оштећеног ткива здравим кардиомиоцитима пореклом од
индукованих плурипотентних ћелија, док је циљ друге стратегије активација
ендогених механизама регенерације. За разлику од човека, неки кичмењаци имају
способност регенерације повређених органа, укључујући срце. Животиња која се
најчешће користи за проучавање регенерације срца је риба зебрица (Danio rerio).
Пионирска студија о изузетном регенеративном капацитету срца зебрице након
ампутације дела коморе објављена је 2002. године, након које је уследило мноштво
публикација о ћелијским и молекуларним механизмима који доприносе
регенеративном одговору. Регенерација срца зебрице је резултат строго регулисане
интеракције већег броја процеса, укључујући инфламаторни одговор,
дедиференцијацију и пролиферацију кардиомиоцита, неоваскуларизацију и
реорганизацију екстраћелијског матрикса. Регенерација се може посматрати као
успаван процес у органима који не регенеришу и манипулацијом овог процеса би
се могла постићи реактивација пролиферације у тим органима након повреде.
Испитивање интеракције између про-регенеришућих механизама и процеса који
утичу на регенеративни капацитет треба да доведе до идентификације фактора
потребних за превазилажење блокаде регенерације. На тај начин би се развиле нове
стратегије за индукцију пролиферације кардиомиоцита и регенерацију срца човека.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Mehanizmi regeneracije srca – šta možemo naučiti od zebrice
T1  - Механизми регенерације срца – шта можемо научити од зебрице
SP  - 288
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1746
ER  - 

@conference{
author = "Kojić, Snežana and Bošković, Srđan and Milovanović, Mina",
year = "2022",
abstract = "Nakon infarkta miokarda povređeno srce čoveka ne može da se regeneriše, već
reaguje formiranjem fibrotičnog ožiljka i remodelovanjem miokarda, koji dovode
do slabljenja njegove funkcije. Miokard sisara je dugo vremena smatran
postmitotičkim i terminalno diferenciranim tkivom. Međutim, kardiomiociti
sisara poseduju ograničenu sposobnost proliferacije. Njihova deoba je redak
događaj i odvija se po veoma niskoj stopi, što svakako nije dovoljno da nadoknadi
milione kardiomiocita trajno izgubljenih usled infarkta miokarda. Trenutno se
u svetu razvijaju dve strategije za podsticanje regeneracije povređenog srca
čoveka kako bi se povratile njegova struktura i funkcija. Jedna strategija
podrazumeva naseljavanje oštećenog tkiva zdravim kardiomiocitima poreklom od
indukovanih pluripotentnih ćelija, dok je cilj druge strategije aktivacija
endogenih mehanizama regeneracije. Za razliku od čoveka, neki kičmenjaci imaju
sposobnost regeneracije povređenih organa, uključujući srce. Životinja koja se
najčešće koristi za proučavanje regeneracije srca je riba zebrica (Danio rerio).
Pionirska studija o izuzetnom regenerativnom kapacitetu srca zebrice nakon
amputacije dela komore objavljena je 2002. godine, nakon koje je usledilo mnoštvo
publikacija o ćelijskim i molekularnim mehanizmima koji doprinose
regenerativnom odgovoru. Regeneracija srca zebrice je rezultat strogo regulisane
interakcije većeg broja procesa, uključujući inflamatorni odgovor,
dediferencijaciju i proliferaciju kardiomiocita, neovaskularizaciju i
reorganizaciju ekstraćelijskog matriksa. Regeneracija se može posmatrati kao
uspavan proces u organima koji ne regenerišu i manipulacijom ovog procesa bi
se mogla postići reaktivacija proliferacije u tim organima nakon povrede.
Ispitivanje interakcije između pro-regenerišućih mehanizama i procesa koji
utiču na regenerativni kapacitet treba da dovede do identifikacije faktora
potrebnih za prevazilaženje blokade regeneracije. Na taj način bi se razvile nove
strategije za indukciju proliferacije kardiomiocita i regeneraciju srca čoveka., Након инфаркта миокарда повређено срце човека не може да се регенерише, већ
реагује формирањем фибротичног ожиљка и ремоделовањем миокарда, који доводе
до слабљења његове функције. Миокард сисара је дуго времена сматран
постмитотичким и терминално диференцираним ткивом. Међутим, кардиомиоцити
сисара поседују ограничену способност пролиферације. Њихова деоба је редак
догађај и одвија се по веома ниској стопи, што свакако није довољно да надокнади
милионе кардиомиоцита трајно изгубљених услед инфаркта миокарда. Тренутно се
у свету развијају две стратегије за подстицање регенерације повређеног срца
човека како би се повратиле његова структура и функција. Једна стратегија
подразумева насељавање оштећеног ткива здравим кардиомиоцитима пореклом од
индукованих плурипотентних ћелија, док је циљ друге стратегије активација
ендогених механизама регенерације. За разлику од човека, неки кичмењаци имају
способност регенерације повређених органа, укључујући срце. Животиња која се
најчешће користи за проучавање регенерације срца је риба зебрица (Danio rerio).
Пионирска студија о изузетном регенеративном капацитету срца зебрице након
ампутације дела коморе објављена је 2002. године, након које је уследило мноштво
публикација о ћелијским и молекуларним механизмима који доприносе
регенеративном одговору. Регенерација срца зебрице је резултат строго регулисане
интеракције већег броја процеса, укључујући инфламаторни одговор,
дедиференцијацију и пролиферацију кардиомиоцита, неоваскуларизацију и
реорганизацију екстраћелијског матрикса. Регенерација се може посматрати као
успаван процес у органима који не регенеришу и манипулацијом овог процеса би
се могла постићи реактивација пролиферације у тим органима након повреде.
Испитивање интеракције између про-регенеришућих механизама и процеса који
утичу на регенеративни капацитет треба да доведе до идентификације фактора
потребних за превазилажење блокаде регенерације. На тај начин би се развиле нове
стратегије за индукцију пролиферације кардиомиоцита и регенерацију срца човека.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Mehanizmi regeneracije srca – šta možemo naučiti od zebrice, Механизми регенерације срца – шта можемо научити од зебрице",
pages = "288",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1746"
}

Kojić, S., Bošković, S.,& Milovanović, M.. (2022). Mehanizmi regeneracije srca – šta možemo naučiti od zebrice. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 288.
https://hdl.handle.net/21.15107/rcub_imagine_1746

Kojić S, Bošković S, Milovanović M. Mehanizmi regeneracije srca – šta možemo naučiti od zebrice. in Treći kongres biologa Srbije. 2022;:288.
https://hdl.handle.net/21.15107/rcub_imagine_1746 .

Kojić, Snežana, Bošković, Srđan, Milovanović, Mina, "Mehanizmi regeneracije srca – šta možemo naučiti od zebrice" in Treći kongres biologa Srbije (2022):288,
https://hdl.handle.net/21.15107/rcub_imagine_1746 .

TY  - THES
AU  - Bošković, Srđan
PY  - 2021
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=8570
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:25620/bdef:Content/download
UR  - https://nardus.mpn.gov.rs/handle/123456789/19043
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/65
AB  - U popriječno-prugastim mišićima članovi familije mišićnih proteina sa ankirinskim ponovcima (eng. Muscle Ankyrin Repeat Proteins, MARP) učestvuju u mehanotransdukciji signala sa sarkomere u jedro, gdje modulišu ekspresiju ciljnih gena. Eksprimirani su tokom razvića srca i skeletnih mišića, povećanog mehaničkog opterećenja ovih organa i u patološkim stanjima poput infarkta miokarda i miopatija. Smatra se da ovi proteini imaju ulogu u adaptaciji mišićne ćelije na stres, ali je ona i dalje nedovoljno istražena. Kako bi upotpunili znanje o MARP familiji, njeni članovi proučavani su u zebrici, model organizmu naročito pogodnom za in vivo analizu razvića, genetičke manipulacije i proučavanje mehanizama regeneracije. Ekspresija ortologa MARP gena zebrice (ankrd1a, ankrd1b i ankrd2) analizirana je tokom razvića i u odgovoru na dvije vrste stresa: povećanu fizičku aktivnost i povredu srca. Generisanje mutanata za MARP gene omogućilo je njihovu funkcionalnu analizu u ovim procesima. Određen je profil ekspresije MARP gena tokom razvića zebrice, od embriona do adulta, a reporterska linija TgBAC(ankrd1a:EGFP) pružila je uvid u ekspresiju ankrd1a sa visokom rezolucijom. Povećana fizička aktivnost indukovala je ekspresiju MARP gena u srcu i skeletnim mišićima zebrice. Ekspresija ankrd1a je povećana u kardiomiocitima graničnog regiona povrede i zavisna od stadijuma remodelovanja miokarda u regenerišućem srcu. Iako mutacije u MARP genima nisu dovele do očigledne promjene fenotipa, kod adultnih jedinki zebrice genotipa ankrd1a-/- primjećena je smanjena proliferacija kardiomiocita nakon povrede srca, ali bez uticaja na konačni ishod regeneracije. Ovim istraživanjem napravljen je ključni korak ka uspostavljanju zebrice kao model ogranizma za proučavanje funkcije članova MARP familije.
AB  - Members of the muscle ankyrin repeat protein (MARP) family in striated muscles play a role in mechanotransduction of signals from the sarcomere to the nucleus, where they modulate expression of target genes. MARPs are expressed during heart and skeletal muscle development, increased mechanical load and in pathological conditions such as myocardial infarction and myopathies. Their presumed role in muscle adaptation to stress remains poorly understood. To extend our knowledge of the MARP family members, they were studied in the zebrafish, a model organism well suited for developmental and regeneration studies, as well as genetic manipulations. Expression of zebrafish MARP gene orthologues (ankrd1a, ankrd1b, ankrd2) was analysed during development and in response to two types of stress: increased physical activity and cardiac injury. Introduction of mutations in the MARP family genes allowed for functional analysis of its members in these processes. Expression profile of MARP genes during zebrafish development was determined in stages from embryo to adult, while the transgenic reporter line TgBAC(ankrd1a:EGFP) provided tracking of ankrd1a expression with higher resolution. Increased physical activity induced the expression of MARP genes in zebrafish heart and skeletal muscles. Expression of ankrd1a was found increased in the injury border zone cardiomyocytes and dependent on myocardium remodelling stage in the regenerating heart. Although mutations in MARP genes did not cause phenotype alterations, a lower rate of cardiomyocytes proliferation was observed in ankrd1a-/- zebrafish following cardiac injury, but with no effect on the regeneration outcome. This study is a key step towards establishing the zebrafish as a model organism for functional studies of the MARP family.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres
T1  - Characterization of zebrafish ankrd1a, ankrd1b and ankrd2 genes in development and stress response
UR  - https://hdl.handle.net/21.15107/rcub_nardus_19043
ER  - 

@phdthesis{
author = "Bošković, Srđan",
year = "2021",
abstract = "U popriječno-prugastim mišićima članovi familije mišićnih proteina sa ankirinskim ponovcima (eng. Muscle Ankyrin Repeat Proteins, MARP) učestvuju u mehanotransdukciji signala sa sarkomere u jedro, gdje modulišu ekspresiju ciljnih gena. Eksprimirani su tokom razvića srca i skeletnih mišića, povećanog mehaničkog opterećenja ovih organa i u patološkim stanjima poput infarkta miokarda i miopatija. Smatra se da ovi proteini imaju ulogu u adaptaciji mišićne ćelije na stres, ali je ona i dalje nedovoljno istražena. Kako bi upotpunili znanje o MARP familiji, njeni članovi proučavani su u zebrici, model organizmu naročito pogodnom za in vivo analizu razvića, genetičke manipulacije i proučavanje mehanizama regeneracije. Ekspresija ortologa MARP gena zebrice (ankrd1a, ankrd1b i ankrd2) analizirana je tokom razvića i u odgovoru na dvije vrste stresa: povećanu fizičku aktivnost i povredu srca. Generisanje mutanata za MARP gene omogućilo je njihovu funkcionalnu analizu u ovim procesima. Određen je profil ekspresije MARP gena tokom razvića zebrice, od embriona do adulta, a reporterska linija TgBAC(ankrd1a:EGFP) pružila je uvid u ekspresiju ankrd1a sa visokom rezolucijom. Povećana fizička aktivnost indukovala je ekspresiju MARP gena u srcu i skeletnim mišićima zebrice. Ekspresija ankrd1a je povećana u kardiomiocitima graničnog regiona povrede i zavisna od stadijuma remodelovanja miokarda u regenerišućem srcu. Iako mutacije u MARP genima nisu dovele do očigledne promjene fenotipa, kod adultnih jedinki zebrice genotipa ankrd1a-/- primjećena je smanjena proliferacija kardiomiocita nakon povrede srca, ali bez uticaja na konačni ishod regeneracije. Ovim istraživanjem napravljen je ključni korak ka uspostavljanju zebrice kao model ogranizma za proučavanje funkcije članova MARP familije., Members of the muscle ankyrin repeat protein (MARP) family in striated muscles play a role in mechanotransduction of signals from the sarcomere to the nucleus, where they modulate expression of target genes. MARPs are expressed during heart and skeletal muscle development, increased mechanical load and in pathological conditions such as myocardial infarction and myopathies. Their presumed role in muscle adaptation to stress remains poorly understood. To extend our knowledge of the MARP family members, they were studied in the zebrafish, a model organism well suited for developmental and regeneration studies, as well as genetic manipulations. Expression of zebrafish MARP gene orthologues (ankrd1a, ankrd1b, ankrd2) was analysed during development and in response to two types of stress: increased physical activity and cardiac injury. Introduction of mutations in the MARP family genes allowed for functional analysis of its members in these processes. Expression profile of MARP genes during zebrafish development was determined in stages from embryo to adult, while the transgenic reporter line TgBAC(ankrd1a:EGFP) provided tracking of ankrd1a expression with higher resolution. Increased physical activity induced the expression of MARP genes in zebrafish heart and skeletal muscles. Expression of ankrd1a was found increased in the injury border zone cardiomyocytes and dependent on myocardium remodelling stage in the regenerating heart. Although mutations in MARP genes did not cause phenotype alterations, a lower rate of cardiomyocytes proliferation was observed in ankrd1a-/- zebrafish following cardiac injury, but with no effect on the regeneration outcome. This study is a key step towards establishing the zebrafish as a model organism for functional studies of the MARP family.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres, Characterization of zebrafish ankrd1a, ankrd1b and ankrd2 genes in development and stress response",
url = "https://hdl.handle.net/21.15107/rcub_nardus_19043"
}

Bošković, S.. (2021). Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_19043

Bošković S. Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_19043 .

Bošković, Srđan, "Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres" (2021),
https://hdl.handle.net/21.15107/rcub_nardus_19043 .

TY  - JOUR
AU  - Bošković, Srđan
AU  - Juez, Ruben Marin
AU  - Stamenković, Nemanja
AU  - Radojković, Dragica
AU  - Stainier, Didier Y. R.
AU  - Kojić, Snežana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1425
AB  - Ankyrin repeat domain 1 (ANKRD1) is a functionally pleiotropic protein found in the nuclei and sarcomeres of cardiac and skeletal muscles, with a proposed role in linking myofibrilar stress and transcriptional regulation. Rapid upregulation of its expression in response to both physiological and pathological stress supports the involvement of ANKRD1 in muscle tissue adaptation and remodeling. However, the exact role of ANKRD1 remains poorly understood. To begin to investigate its function at higher resolution, we have generated and characterized a TgBAC(ankrd1a:EGFP) zebrafish line. This reporter line displays transgene expression in slow skeletal muscle fibers during development and exercise responsiveness in adult cardiac muscle. To better understand the role of Ankrd1a in pathological conditions in adult zebrafish, we assessed ankrd1a expression after cardiac ventricle cryoinjury and observed localized upregulation in cardiomyocytes in the border zone. We show that this expression in injured hearts is recapitulated by the TgBAC(ankrd1a:EGFP) reporter. Our results identify novel expression domains of ankrd1a and suggest an important role for Ankrd1a in the early stress response and regeneration of cardiac tissue. This new reporter line will help decipher the role of Ankrd1a in striated muscle stress response, including after cardiac injury.
PB  - Elsevier, Amsterdam
T2  - Gene
T1  - The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish
VL  - 792
DO  - 10.1016/j.gene.2021.145725
ER  - 

@article{
author = "Bošković, Srđan and Juez, Ruben Marin and Stamenković, Nemanja and Radojković, Dragica and Stainier, Didier Y. R. and Kojić, Snežana",
year = "2021",
abstract = "Ankyrin repeat domain 1 (ANKRD1) is a functionally pleiotropic protein found in the nuclei and sarcomeres of cardiac and skeletal muscles, with a proposed role in linking myofibrilar stress and transcriptional regulation. Rapid upregulation of its expression in response to both physiological and pathological stress supports the involvement of ANKRD1 in muscle tissue adaptation and remodeling. However, the exact role of ANKRD1 remains poorly understood. To begin to investigate its function at higher resolution, we have generated and characterized a TgBAC(ankrd1a:EGFP) zebrafish line. This reporter line displays transgene expression in slow skeletal muscle fibers during development and exercise responsiveness in adult cardiac muscle. To better understand the role of Ankrd1a in pathological conditions in adult zebrafish, we assessed ankrd1a expression after cardiac ventricle cryoinjury and observed localized upregulation in cardiomyocytes in the border zone. We show that this expression in injured hearts is recapitulated by the TgBAC(ankrd1a:EGFP) reporter. Our results identify novel expression domains of ankrd1a and suggest an important role for Ankrd1a in the early stress response and regeneration of cardiac tissue. This new reporter line will help decipher the role of Ankrd1a in striated muscle stress response, including after cardiac injury.",
publisher = "Elsevier, Amsterdam",
journal = "Gene",
title = "The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish",
volume = "792",
doi = "10.1016/j.gene.2021.145725"
}

Bošković, S., Juez, R. M., Stamenković, N., Radojković, D., Stainier, D. Y. R.,& Kojić, S.. (2021). The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish. in Gene
Elsevier, Amsterdam., 792.
https://doi.org/10.1016/j.gene.2021.145725

Bošković S, Juez RM, Stamenković N, Radojković D, Stainier DYR, Kojić S. The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish. in Gene. 2021;792.
doi:10.1016/j.gene.2021.145725 .

Bošković, Srđan, Juez, Ruben Marin, Stamenković, Nemanja, Radojković, Dragica, Stainier, Didier Y. R., Kojić, Snežana, "The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish" in Gene, 792 (2021),
https://doi.org/10.1016/j.gene.2021.145725 . .

TY  - JOUR
AU  - Stamenković, Nemanja
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Bošković, Srđan
AU  - Kojić, Ana
AU  - Popić, Kristina
AU  - Stanković, Marija
AU  - Wang, Yajun
AU  - Milenković, Sanja
AU  - Radojković, Dragica
AU  - Ma, Guada
AU  - Kojić, Snežana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1307
AB  - Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chickenANKRD2. The chickenANKRD2coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative-glycolytic, type IIA myofibers. Association of chickenANKRD2with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests thatANKRD2is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus
EP  - 396
IS  - 4
SP  - 383
VL  - 154
DO  - 10.1007/s00418-020-01899-1
ER  - 

@article{
author = "Stamenković, Nemanja and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Bošković, Srđan and Kojić, Ana and Popić, Kristina and Stanković, Marija and Wang, Yajun and Milenković, Sanja and Radojković, Dragica and Ma, Guada and Kojić, Snežana",
year = "2020",
abstract = "Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chickenANKRD2. The chickenANKRD2coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative-glycolytic, type IIA myofibers. Association of chickenANKRD2with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests thatANKRD2is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus",
pages = "396-383",
number = "4",
volume = "154",
doi = "10.1007/s00418-020-01899-1"
}

Stamenković, N., Jasnić, J., Novković, M., Milošević, E., Bošković, S., Kojić, A., Popić, K., Stanković, M., Wang, Y., Milenković, S., Radojković, D., Ma, G.,& Kojić, S.. (2020). Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus. in Histochemistry and Cell Biology
Springer, New York., 154(4), 383-396.
https://doi.org/10.1007/s00418-020-01899-1

Stamenković N, Jasnić J, Novković M, Milošević E, Bošković S, Kojić A, Popić K, Stanković M, Wang Y, Milenković S, Radojković D, Ma G, Kojić S. Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus. in Histochemistry and Cell Biology. 2020;154(4):383-396.
doi:10.1007/s00418-020-01899-1 .

Stamenković, Nemanja, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Bošković, Srđan, Kojić, Ana, Popić, Kristina, Stanković, Marija, Wang, Yajun, Milenković, Sanja, Radojković, Dragica, Ma, Guada, Kojić, Snežana, "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus" in Histochemistry and Cell Biology, 154, no. 4 (2020):383-396,
https://doi.org/10.1007/s00418-020-01899-1 . .

TY  - JOUR
AU  - Bošković, Srđan
AU  - Marin-Juez, Ruben
AU  - Jasnić, Jovana
AU  - Reischauer, Sven
AU  - El Sammak, Hadil
AU  - Kojić, Ana
AU  - Faulkner, Georgine
AU  - Radojković, Dragica
AU  - Stainier, Didier Y. R.
AU  - Kojić, Snežana
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1141
AB  - Muscle proteins with ankyrin repeats (MARPs) ANKRD1 and ANKRD2 are titin-associated proteins with a putative role as transcriptional co-regulators in striated muscle, involved in the cellular response to mechanical, oxidative and metabolic stress. Since many aspects of the biology of MARPs, particularly exact mechanisms of their action, in striated muscle are still elusive, research in this field will benefit from novel animal model system. Here we investigated the MARPs found in zebrafish for protein structure, evolutionary conservation, spatiotemporal expression profiles and response to increased muscle activity. Ankrd1 and Ankrd2 show overall moderate conservation at the protein level, more pronounced in the region of ankyrin repeats, motifs indispensable for their function. The two zebrafish genes, ankrd1a and ankrd1b, counterparts of mammalian ANKRD1/Ankrd1, have different expression profiles during first seven days of development. Mild increase of ankrd1a transcript levels was detected at 72 hpf (1.74 +/- 0.24 fold increase relative to 24 hpf time point), while ankrd1b expression was markedly upregulated from 24 hpf onward and peaked at 72 hpf (92.18 +/- 36.95 fold increase relative to 24 hpf time point). Spatially, they exhibited non-overlapping expression patterns during skeletal muscle development in trunk (ankrd1a) and tail (ankrd1b) somites. Expression of ankrd2 was barely detectable. Zebrafish MARPs, expressed at a relatively low level in adult striated muscle, were found to be responsive to endurance exercise training consisting of two bouts of 3 hours of forced swimming daily, for five consecutive days. Three hours after the last exercise bout, ankrd1a expression increased in cardiac muscle (6.19 +/- 5.05 fold change), while ankrd1b and ankrd2 were upregulated in skeletal muscle (1.97 +/- 1.05 and 1.84 +/- 0.58 fold change, respectively). This study provides the foundation to establish zebrafish as a novel in vivo model for further investigation of MARPs function in striated muscle.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise
IS  - 9
VL  - 13
DO  - 10.1371/journal.pone.0204312
ER  - 

@article{
author = "Bošković, Srđan and Marin-Juez, Ruben and Jasnić, Jovana and Reischauer, Sven and El Sammak, Hadil and Kojić, Ana and Faulkner, Georgine and Radojković, Dragica and Stainier, Didier Y. R. and Kojić, Snežana",
year = "2018",
abstract = "Muscle proteins with ankyrin repeats (MARPs) ANKRD1 and ANKRD2 are titin-associated proteins with a putative role as transcriptional co-regulators in striated muscle, involved in the cellular response to mechanical, oxidative and metabolic stress. Since many aspects of the biology of MARPs, particularly exact mechanisms of their action, in striated muscle are still elusive, research in this field will benefit from novel animal model system. Here we investigated the MARPs found in zebrafish for protein structure, evolutionary conservation, spatiotemporal expression profiles and response to increased muscle activity. Ankrd1 and Ankrd2 show overall moderate conservation at the protein level, more pronounced in the region of ankyrin repeats, motifs indispensable for their function. The two zebrafish genes, ankrd1a and ankrd1b, counterparts of mammalian ANKRD1/Ankrd1, have different expression profiles during first seven days of development. Mild increase of ankrd1a transcript levels was detected at 72 hpf (1.74 +/- 0.24 fold increase relative to 24 hpf time point), while ankrd1b expression was markedly upregulated from 24 hpf onward and peaked at 72 hpf (92.18 +/- 36.95 fold increase relative to 24 hpf time point). Spatially, they exhibited non-overlapping expression patterns during skeletal muscle development in trunk (ankrd1a) and tail (ankrd1b) somites. Expression of ankrd2 was barely detectable. Zebrafish MARPs, expressed at a relatively low level in adult striated muscle, were found to be responsive to endurance exercise training consisting of two bouts of 3 hours of forced swimming daily, for five consecutive days. Three hours after the last exercise bout, ankrd1a expression increased in cardiac muscle (6.19 +/- 5.05 fold change), while ankrd1b and ankrd2 were upregulated in skeletal muscle (1.97 +/- 1.05 and 1.84 +/- 0.58 fold change, respectively). This study provides the foundation to establish zebrafish as a novel in vivo model for further investigation of MARPs function in striated muscle.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise",
number = "9",
volume = "13",
doi = "10.1371/journal.pone.0204312"
}

Bošković, S., Marin-Juez, R., Jasnić, J., Reischauer, S., El Sammak, H., Kojić, A., Faulkner, G., Radojković, D., Stainier, D. Y. R.,& Kojić, S.. (2018). Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise. in PLoS One
Public Library Science, San Francisco., 13(9).
https://doi.org/10.1371/journal.pone.0204312

Bošković S, Marin-Juez R, Jasnić J, Reischauer S, El Sammak H, Kojić A, Faulkner G, Radojković D, Stainier DYR, Kojić S. Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise. in PLoS One. 2018;13(9).
doi:10.1371/journal.pone.0204312 .

Bošković, Srđan, Marin-Juez, Ruben, Jasnić, Jovana, Reischauer, Sven, El Sammak, Hadil, Kojić, Ana, Faulkner, Georgine, Radojković, Dragica, Stainier, Didier Y. R., Kojić, Snežana, "Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise" in PLoS One, 13, no. 9 (2018),
https://doi.org/10.1371/journal.pone.0204312 . .

TY  - JOUR
AU  - Bartolome, Aleksandar
AU  - Bošković, Srđan
AU  - Paunović, Ivan
AU  - Bozić, Vesna
AU  - Cvejić, Dubravka
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/987
AB  - Stomatin-like protein 2 (SLP-2), a member of the stomatin protein family, has emerged as a potential molecular hallmark of tumor progression in several human malignancies. The aim of this study was to analyze SLP-2 expression pattern in benign and malignant thyroid tumors (n = 210) and to examine its relationship with clinicopathological parameters and BRAFV600E mutation in thyroid cancer. SLP-2 immunohistochemical expression was not detected in benign adenomas and was absent/weak in follicular and anaplastic carcinomas. High expression levels of SLP-2, found only in papillary thyroid carcinoma (PTC), particularly in the classical variant, were significantly associated with adverse clinicopathological parameters: lymph node metastasis (p = 0.002), extrathyroid invasion (p  lt  0.001), pT status (p  lt  0.001), and advanced tumor stage (p = 0.001). Additional genotyping of PTC cases for the BRAFV600E mutation revealed for the first time a close relation between SLP-2 overexpression and the presence of BRAF mutation (p = 0.02) with high positive rates of lymph node metastasis (70%) and extrathyroid invasion (80%) in these cases. The significant association of SLP-2 overexpression with unfavorable clinicopathological characteristics and BRAFV600E mutation indicates that SLP-2 may have a role in aggressiveness of BRAF-mutated PTC and that SLP-2 evaluation could be clinically useful in identification of high-risk PTC patients.
PB  - Wiley-Blackwell, Hoboken
T2  - APMIS
T1  - Stomatin-like protein 2 overexpression in papillary thyroid carcinoma is significantly associated with high-risk clinicopathological parameters and BRAFV600E mutation
EP  - 277
IS  - 4
SP  - 271
VL  - 124
DO  - 10.1111/apm.12505
ER  - 

@article{
author = "Bartolome, Aleksandar and Bošković, Srđan and Paunović, Ivan and Bozić, Vesna and Cvejić, Dubravka",
year = "2016",
abstract = "Stomatin-like protein 2 (SLP-2), a member of the stomatin protein family, has emerged as a potential molecular hallmark of tumor progression in several human malignancies. The aim of this study was to analyze SLP-2 expression pattern in benign and malignant thyroid tumors (n = 210) and to examine its relationship with clinicopathological parameters and BRAFV600E mutation in thyroid cancer. SLP-2 immunohistochemical expression was not detected in benign adenomas and was absent/weak in follicular and anaplastic carcinomas. High expression levels of SLP-2, found only in papillary thyroid carcinoma (PTC), particularly in the classical variant, were significantly associated with adverse clinicopathological parameters: lymph node metastasis (p = 0.002), extrathyroid invasion (p  lt  0.001), pT status (p  lt  0.001), and advanced tumor stage (p = 0.001). Additional genotyping of PTC cases for the BRAFV600E mutation revealed for the first time a close relation between SLP-2 overexpression and the presence of BRAF mutation (p = 0.02) with high positive rates of lymph node metastasis (70%) and extrathyroid invasion (80%) in these cases. The significant association of SLP-2 overexpression with unfavorable clinicopathological characteristics and BRAFV600E mutation indicates that SLP-2 may have a role in aggressiveness of BRAF-mutated PTC and that SLP-2 evaluation could be clinically useful in identification of high-risk PTC patients.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "APMIS",
title = "Stomatin-like protein 2 overexpression in papillary thyroid carcinoma is significantly associated with high-risk clinicopathological parameters and BRAFV600E mutation",
pages = "277-271",
number = "4",
volume = "124",
doi = "10.1111/apm.12505"
}

Bartolome, A., Bošković, S., Paunović, I., Bozić, V.,& Cvejić, D.. (2016). Stomatin-like protein 2 overexpression in papillary thyroid carcinoma is significantly associated with high-risk clinicopathological parameters and BRAFV600E mutation. in APMIS
Wiley-Blackwell, Hoboken., 124(4), 271-277.
https://doi.org/10.1111/apm.12505

Bartolome A, Bošković S, Paunović I, Bozić V, Cvejić D. Stomatin-like protein 2 overexpression in papillary thyroid carcinoma is significantly associated with high-risk clinicopathological parameters and BRAFV600E mutation. in APMIS. 2016;124(4):271-277.
doi:10.1111/apm.12505 .

Bartolome, Aleksandar, Bošković, Srđan, Paunović, Ivan, Bozić, Vesna, Cvejić, Dubravka, "Stomatin-like protein 2 overexpression in papillary thyroid carcinoma is significantly associated with high-risk clinicopathological parameters and BRAFV600E mutation" in APMIS, 124, no. 4 (2016):271-277,
https://doi.org/10.1111/apm.12505 . .

TY  - JOUR
AU  - Jasnić, Jovana
AU  - Krause, Sabine
AU  - Savić, Slobodan
AU  - Kojić, Ana
AU  - Kovcić, Vlado
AU  - Bošković, Srđan
AU  - Nestorović, Aleksandra
AU  - Rakićević, Ljiljana
AU  - Schreiber-Katz, Olivia
AU  - Vogel, Johannes G.
AU  - Schoser, Benedikt G.
AU  - Walter, Maggie C.
AU  - Valle, Giorgio
AU  - Radojković, Dragica
AU  - Faulkner, Georgine
AU  - Kojić, Snežana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/933
AB  - Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles
EP  - 584
IS  - 5
SP  - 569
VL  - 146
DO  - 10.1007/s00418-016-1465-0
ER  - 

@article{
author = "Jasnić, Jovana and Krause, Sabine and Savić, Slobodan and Kojić, Ana and Kovcić, Vlado and Bošković, Srđan and Nestorović, Aleksandra and Rakićević, Ljiljana and Schreiber-Katz, Olivia and Vogel, Johannes G. and Schoser, Benedikt G. and Walter, Maggie C. and Valle, Giorgio and Radojković, Dragica and Faulkner, Georgine and Kojić, Snežana",
year = "2016",
abstract = "Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles",
pages = "584-569",
number = "5",
volume = "146",
doi = "10.1007/s00418-016-1465-0"
}

Jasnić, J., Krause, S., Savić, S., Kojić, A., Kovcić, V., Bošković, S., Nestorović, A., Rakićević, L., Schreiber-Katz, O., Vogel, J. G., Schoser, B. G., Walter, M. C., Valle, G., Radojković, D., Faulkner, G.,& Kojić, S.. (2016). Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles. in Histochemistry and Cell Biology
Springer, New York., 146(5), 569-584.
https://doi.org/10.1007/s00418-016-1465-0

Jasnić J, Krause S, Savić S, Kojić A, Kovcić V, Bošković S, Nestorović A, Rakićević L, Schreiber-Katz O, Vogel JG, Schoser BG, Walter MC, Valle G, Radojković D, Faulkner G, Kojić S. Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles. in Histochemistry and Cell Biology. 2016;146(5):569-584.
doi:10.1007/s00418-016-1465-0 .

Jasnić, Jovana, Krause, Sabine, Savić, Slobodan, Kojić, Ana, Kovcić, Vlado, Bošković, Srđan, Nestorović, Aleksandra, Rakićević, Ljiljana, Schreiber-Katz, Olivia, Vogel, Johannes G., Schoser, Benedikt G., Walter, Maggie C., Valle, Giorgio, Radojković, Dragica, Faulkner, Georgine, Kojić, Snežana, "Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles" in Histochemistry and Cell Biology, 146, no. 5 (2016):569-584,
https://doi.org/10.1007/s00418-016-1465-0 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Milosević, Katarina
AU  - Bošković, Srđan
AU  - Nestorović, Branimir
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/709
AB  - The aim of this study was to investigate polymorphisms in the promoter region of the neutrophil elastase (ELANE) gene as potential modulators of the therapeutic response in children with idiopathic bronchiectasis. The study included 48 children between 5 and 17 years old who were diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of the thorax. In all patients therapy included administration of antibiotics, anti-inflammatory drugs, expectorants, and postural drainage. Response to therapy was evaluated by the change in FeNO levels before and after administration of therapy. The ELANE promoter region polymorphisms were analyzed by PCR-direct DNA sequencing. According to the predicted activity of ELANE genotypes, subjects were divided into two groups: low/intermediate activity (n = 18) and high activity (n = 30). Subjects in the group with high-activity genotype had higher initial FeNO levels and this difference was statistically significant (t = 2.906; p = 0.006). The difference between FeNO levels before and after therapy was also statistically significantly higher in children with high-activity genotype (t = 3.329; p = 0.002). Statistically significant correlation was observed between the change in FeNO levels and ELANE genotypes (r = 0.350; p = 0.015). Children with high-activity genotype had higher initial FeNO levels and showed better response to therapy than children with low/intermediate-activity genotypes.
PB  - Springer, New York
T2  - Lung
T1  - Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?
EP  - 599
IS  - 4
SP  - 595
VL  - 192
DO  - 10.1007/s00408-014-9596-3
ER  - 

@article{
author = "Nikolić, Aleksandra and Milosević, Katarina and Bošković, Srđan and Nestorović, Branimir",
year = "2014",
abstract = "The aim of this study was to investigate polymorphisms in the promoter region of the neutrophil elastase (ELANE) gene as potential modulators of the therapeutic response in children with idiopathic bronchiectasis. The study included 48 children between 5 and 17 years old who were diagnosed with idiopathic bronchiectasis based on high-resolution computed tomography of the thorax. In all patients therapy included administration of antibiotics, anti-inflammatory drugs, expectorants, and postural drainage. Response to therapy was evaluated by the change in FeNO levels before and after administration of therapy. The ELANE promoter region polymorphisms were analyzed by PCR-direct DNA sequencing. According to the predicted activity of ELANE genotypes, subjects were divided into two groups: low/intermediate activity (n = 18) and high activity (n = 30). Subjects in the group with high-activity genotype had higher initial FeNO levels and this difference was statistically significant (t = 2.906; p = 0.006). The difference between FeNO levels before and after therapy was also statistically significantly higher in children with high-activity genotype (t = 3.329; p = 0.002). Statistically significant correlation was observed between the change in FeNO levels and ELANE genotypes (r = 0.350; p = 0.015). Children with high-activity genotype had higher initial FeNO levels and showed better response to therapy than children with low/intermediate-activity genotypes.",
publisher = "Springer, New York",
journal = "Lung",
title = "Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?",
pages = "599-595",
number = "4",
volume = "192",
doi = "10.1007/s00408-014-9596-3"
}

Nikolić, A., Milosević, K., Bošković, S.,& Nestorović, B.. (2014). Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?. in Lung
Springer, New York., 192(4), 595-599.
https://doi.org/10.1007/s00408-014-9596-3

Nikolić A, Milosević K, Bošković S, Nestorović B. Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?. in Lung. 2014;192(4):595-599.
doi:10.1007/s00408-014-9596-3 .

Nikolić, Aleksandra, Milosević, Katarina, Bošković, Srđan, Nestorović, Branimir, "Neutrophil Elastase Gene Polymorphisms: Modulators of Response to Therapy in Childhood Bronchiectasis?" in Lung, 192, no. 4 (2014):595-599,
https://doi.org/10.1007/s00408-014-9596-3 . .

TY  - CONF
AU  - Milosević, Katarina
AU  - Bošković, Srđan
AU  - Ljujić, Mila
AU  - Nestorović, Branimir
AU  - Nikolić, Aleksandra
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/675
PB  - European Respiratory Soc Journals Ltd, Sheffield
C3  - European Respiratory Journal
T1  - Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_imagine_675
ER  - 

@conference{
author = "Milosević, Katarina and Bošković, Srđan and Ljujić, Mila and Nestorović, Branimir and Nikolić, Aleksandra",
year = "2013",
publisher = "European Respiratory Soc Journals Ltd, Sheffield",
journal = "European Respiratory Journal",
title = "Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_imagine_675"
}

Milosević, K., Bošković, S., Ljujić, M., Nestorović, B.,& Nikolić, A.. (2013). Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis. in European Respiratory Journal
European Respiratory Soc Journals Ltd, Sheffield., 42.
https://hdl.handle.net/21.15107/rcub_imagine_675

Milosević K, Bošković S, Ljujić M, Nestorović B, Nikolić A. Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis. in European Respiratory Journal. 2013;42.
https://hdl.handle.net/21.15107/rcub_imagine_675 .

Milosević, Katarina, Bošković, Srđan, Ljujić, Mila, Nestorović, Branimir, Nikolić, Aleksandra, "Neutrophil elastase gene polymorphisms as therapy modulators in childhood bronchiectasis" in European Respiratory Journal, 42 (2013),
https://hdl.handle.net/21.15107/rcub_imagine_675 .