TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Cosović, Vladan
AU  - Atanase, Leonard Ionut
AU  - Freitas, Filomena
AU  - Matos, Mariana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1714
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
IS  - 3
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Cosović, Vladan and Atanase, Leonard Ionut and Freitas, Filomena and Matos, Mariana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
number = "3",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Cosović, V., Atanase, L. I., Freitas, F., Matos, M.,& Nikodinović-Runić, J.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences, 24(3), 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Cosović V, Atanase LI, Freitas F, Matos M, Nikodinović-Runić J. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24(3):1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Cosović, Vladan, Atanase, Leonard Ionut, Freitas, Filomena, Matos, Mariana, Nikodinović-Runić, Jasmina, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24, no. 3 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - JOUR
AU  - Aleksić, Jelena M.
AU  - Banović Đeri, Bojana
AU  - Miljuš-Đukić, Jovanka
AU  - Jovanović, Živko
AU  - Mikić, Aleksandar
AU  - Cupina, Branko
AU  - Zlatković, Bojan
AU  - Anđelković, Snežana
AU  - Spanu, Ilaria
AU  - Jelić, Mihailo
AU  - Maksimović, Vesna R.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/834
AB  - Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Genetics and Plant Breeding
T1  - A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba
EP  - 39
IS  - 1
SP  - 36
VL  - 51
DO  - 10.17221/153/2014-CJGPB
ER  - 

@article{
author = "Aleksić, Jelena M. and Banović Đeri, Bojana and Miljuš-Đukić, Jovanka and Jovanović, Živko and Mikić, Aleksandar and Cupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Spanu, Ilaria and Jelić, Mihailo and Maksimović, Vesna R.",
year = "2015",
abstract = "Although more than 400 microsatellite loci are currently available for Vicia faba L. (faba bean), an important food and feed grain crop legume, they have not yet been used for comprehensive molecular characterization of this crop. We report a three-step procedure for rapid and cost-effective delineation and utilization of informative genomic nuclear SSRs for paralleled genotyping in faba bean suitable also for other species: (i) pre-selection of loci generating PCR products of expected lengths which are potentially polymorphic (achieved by PCR amplification in bulked samples); (ii) exclusion of loci burdened with persistent null alleles and multilocus amplification products (based on PCR amplification of pre-selected loci in individual genotypes), and (iii) multiplexing. We demonstrate also that genomic SSRs are promising molecular tools for molecular characterization of faba bean required also for crop improvement.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Genetics and Plant Breeding",
title = "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba",
pages = "39-36",
number = "1",
volume = "51",
doi = "10.17221/153/2014-CJGPB"
}

Aleksić, J. M., Banović Đeri, B., Miljuš-Đukić, J., Jovanović, Ž., Mikić, A., Cupina, B., Zlatković, B., Anđelković, S., Spanu, I., Jelić, M.,& Maksimović, V. R.. (2015). A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding
Czech Academy Agricultural Sciences, Prague., 51(1), 36-39.
https://doi.org/10.17221/153/2014-CJGPB

Aleksić JM, Banović Đeri B, Miljuš-Đukić J, Jovanović Ž, Mikić A, Cupina B, Zlatković B, Anđelković S, Spanu I, Jelić M, Maksimović VR. A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba. in Czech Journal of Genetics and Plant Breeding. 2015;51(1):36-39.
doi:10.17221/153/2014-CJGPB .

Aleksić, Jelena M., Banović Đeri, Bojana, Miljuš-Đukić, Jovanka, Jovanović, Živko, Mikić, Aleksandar, Cupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Spanu, Ilaria, Jelić, Mihailo, Maksimović, Vesna R., "A Rapid and Cost-effective Procedure for Delineation and Utilization of Genomic Microsatellites for Paralleled Genotyping in Vicia faba" in Czech Journal of Genetics and Plant Breeding, 51, no. 1 (2015):36-39,
https://doi.org/10.17221/153/2014-CJGPB . .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Maksimović, Vesna R.
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/742
AB  - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - Plant Physiology and Biochemistry
T1  - Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)
EP  - 31
SP  - 26
VL  - 83
DO  - 10.1016/j.plaphy.2014.07.008
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Mikić, Aleksandar and Radović, Svetlana and Maksimović, Vesna R.",
year = "2014",
abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at post-transcriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that they could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame reflects the changes as close as possible to the changes where water stress causes visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target - genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. To the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) did not change in roots and shoots of water-stressed plants, compared to control (well) hydrated plants. This suggests that COX5b is not the target of miR398, or that its expression is regulated by some other mechanism. P-1B-ATPase expression increased during water deficit only in the shoots of pea; in the roots there were no changes in expression. Our results help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "Plant Physiology and Biochemistry",
title = "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)",
pages = "31-26",
volume = "83",
doi = "10.1016/j.plaphy.2014.07.008"
}

Jovanović, Ž., Stanisavljević, N., Mikić, A., Radović, S.,& Maksimović, V. R.. (2014). Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 83, 26-31.
https://doi.org/10.1016/j.plaphy.2014.07.008

Jovanović Ž, Stanisavljević N, Mikić A, Radović S, Maksimović VR. Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.). in Plant Physiology and Biochemistry. 2014;83:26-31.
doi:10.1016/j.plaphy.2014.07.008 .

Jovanović, Živko, Stanisavljević, Nemanja, Mikić, Aleksandar, Radović, Svetlana, Maksimović, Vesna R., "Water deficit down-regulates miR398 and miR408 in pea (Pisum sativum L.)" in Plant Physiology and Biochemistry, 83 (2014):26-31,
https://doi.org/10.1016/j.plaphy.2014.07.008 . .

TY  - JOUR
AU  - Miljuš-Đukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Radović, S.
AU  - Jovanović, Živko
AU  - Mikić, A.
AU  - Maksimović, Vesna R.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/688
AB  - Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.
T2  - Australian Journal of Crop Science
T1  - Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression
EP  - 2153
IS  - 13
SP  - 2145
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_imagine_688
ER  - 

@article{
author = "Miljuš-Đukić, Jovanka and Stanisavljević, Nemanja and Radović, S. and Jovanović, Živko and Mikić, A. and Maksimović, Vesna R.",
year = "2013",
abstract = "Soil salinity is one of the major abiotic stresses leading to crops yield failure. For investigation of salinity response in pea, in vitro cultures of three pea species (Pisum arvense, P. sativum and P. fulvum) were induced. Regenerated shoots of these pea species were grown on a medium with moderate and high NaCl concentrations (75, 120, 150 and 200 mM NaCl) to compare the antioxidative capacity in response to salt stress. Growth reduction was observed in all species and it correlated with the increase of NaCl concentration. After four weeks of treatment, the crude tissue extracts were used for measuring the stress response parameters. Level of lipid peroxidation increased in all three species, but in P. fulvum the effect of NaCl was less pronounced. Total chlorophyll and phenolic content showed differences among the three species, while the DPPH-scavenging activity was the most evident in P. fulvum. The northern blot analysis of the miRNA398 expression showed a similar pattern between P. sativum and P. arvense but different to that of P. fulvum, as in P. fulvum miRNA 398 was only expressed at moderate NaCl concentration, with total inhibition at high concentrations. On the basis of these results it can be concluded that P. fulvum could represent a gene pool for improving P. sativum and P. arvense stress defence capacity.",
journal = "Australian Journal of Crop Science",
title = "Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression",
pages = "2153-2145",
number = "13",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_imagine_688"
}

Miljuš-Đukić, J., Stanisavljević, N., Radović, S., Jovanović, Ž., Mikić, A.,& Maksimović, V. R.. (2013). Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression. in Australian Journal of Crop Science, 7(13), 2145-2153.
https://hdl.handle.net/21.15107/rcub_imagine_688

Miljuš-Đukić J, Stanisavljević N, Radović S, Jovanović Ž, Mikić A, Maksimović VR. Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression. in Australian Journal of Crop Science. 2013;7(13):2145-2153.
https://hdl.handle.net/21.15107/rcub_imagine_688 .

Miljuš-Đukić, Jovanka, Stanisavljević, Nemanja, Radović, S., Jovanović, Živko, Mikić, A., Maksimović, Vesna R., "Differential response of three contrasting pea (Pisum arvense, P. Sativum and P. Fulvum) species to salt stress: Assessment of variation in antioxidative defence and miRNA expression" in Australian Journal of Crop Science, 7, no. 13 (2013):2145-2153,
https://hdl.handle.net/21.15107/rcub_imagine_688 .

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Mikić, A.
AU  - Radović, S.
AU  - Maksimović, Vesna R.
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/684
AB  - Protein pea (Pisum sativum L.) is an agronomic type of pea introduced in the region of modern Serbia in the early eighties of the last century. In this study, a new variety NS MRAZ developed by pedigree selection in 2011 was used. Two-week-old plants were subjected to drought stress by withholding irrigation for 7 and 10 days, and rehydrated for one day. Stress effects were monitored by determination of relative water content (RWC), lipid peroxidation and accumulation of reactive oxygen species (ROS).We isolated partial cDNA of Pisum sativum DREB2A, namely PsDREB2A (HM229349), which belongs to the DREB gene family. Bioinformatics analyses showed high similarity with DREB2A gene from model legume Medicago truncataula. The relationship between the expression profile of PsDREB2A gene and water stress was assayed by quantitative real time PCR in pea roots and leaves. According to obtained results, it is evident that loss of water content strongly induced accumulation of ROS and lipid peroxidation in pea plants. The expression of PsDREB2A in pea roots increased with water content decrease, reaching maximum after 10 days of dehydration (2 fold higher than in control plants). On the other hand, in the pea leaves, the highest level of expression was observed after 7 days of dehydration (60% higher than in control). Observed tissue-specific expression profile of PsDREB2A suggests complex regulation and the role of this transcription factor in pea drought response. In addition, we can conclude that this pea (var." NS MRAZ") is a drought sensitive plant.
T2  - Australian Journal of Crop Science
T1  - The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress
EP  - 1596
IS  - 10
SP  - 1590
VL  - 7
UR  - https://hdl.handle.net/21.15107/rcub_imagine_684
ER  - 

@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Mikić, A. and Radović, S. and Maksimović, Vesna R.",
year = "2013",
abstract = "Protein pea (Pisum sativum L.) is an agronomic type of pea introduced in the region of modern Serbia in the early eighties of the last century. In this study, a new variety NS MRAZ developed by pedigree selection in 2011 was used. Two-week-old plants were subjected to drought stress by withholding irrigation for 7 and 10 days, and rehydrated for one day. Stress effects were monitored by determination of relative water content (RWC), lipid peroxidation and accumulation of reactive oxygen species (ROS).We isolated partial cDNA of Pisum sativum DREB2A, namely PsDREB2A (HM229349), which belongs to the DREB gene family. Bioinformatics analyses showed high similarity with DREB2A gene from model legume Medicago truncataula. The relationship between the expression profile of PsDREB2A gene and water stress was assayed by quantitative real time PCR in pea roots and leaves. According to obtained results, it is evident that loss of water content strongly induced accumulation of ROS and lipid peroxidation in pea plants. The expression of PsDREB2A in pea roots increased with water content decrease, reaching maximum after 10 days of dehydration (2 fold higher than in control plants). On the other hand, in the pea leaves, the highest level of expression was observed after 7 days of dehydration (60% higher than in control). Observed tissue-specific expression profile of PsDREB2A suggests complex regulation and the role of this transcription factor in pea drought response. In addition, we can conclude that this pea (var." NS MRAZ") is a drought sensitive plant.",
journal = "Australian Journal of Crop Science",
title = "The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress",
pages = "1596-1590",
number = "10",
volume = "7",
url = "https://hdl.handle.net/21.15107/rcub_imagine_684"
}

Jovanović, Ž., Stanisavljević, N., Mikić, A., Radović, S.,& Maksimović, V. R.. (2013). The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress. in Australian Journal of Crop Science, 7(10), 1590-1596.
https://hdl.handle.net/21.15107/rcub_imagine_684

Jovanović Ž, Stanisavljević N, Mikić A, Radović S, Maksimović VR. The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress. in Australian Journal of Crop Science. 2013;7(10):1590-1596.
https://hdl.handle.net/21.15107/rcub_imagine_684 .

Jovanović, Živko, Stanisavljević, Nemanja, Mikić, A., Radović, S., Maksimović, Vesna R., "The expression of drought responsive element binding protein (DREB2A) related gene from pea (Oisum sativum L.) as affected by water stress" in Australian Journal of Crop Science, 7, no. 10 (2013):1590-1596,
https://hdl.handle.net/21.15107/rcub_imagine_684 .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
AU  - Nikolić, Dragana
AU  - Samardžić, Jelena
AU  - Maksimović, Vesna R.
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/496
AB  - BY-2, ćelijska linija duvana, upotrebljena je za produkciju novog tipa aspartatne proteinaze heljde - FeAPL1-His6. Potvrđena je očekivana pepstatin-A senzitivna enzimska aktivnost ovog proteina na pH 3,0. Lokalizacija proteina FeAPL1-His6 je određena imunocitohemijski i uporednom analizom Western blot transformisanih ćelija BY-2 i njihovih protoplasta. Ovim metodama je utvrđeno da ispitivani protein ima ekstracelularnu lokalizaciju u ćelijskom zidu. Na osnovu dobijenih rezultata razmatrana je potencijalna funkcija proteaze FeAPL1.
AB  - The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extracellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained results, potential functions of FeAPL1 are discussed.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana
T1  - Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells
EP  - 1236
IS  - 9
SP  - 1229
VL  - 76
DO  - 10.2298/JSC110120106M
ER  - 

@article{
author = "Milisavljević, Mira and Timotijević, Gordana and Nikolić, Dragana and Samardžić, Jelena and Maksimović, Vesna R.",
year = "2011",
abstract = "BY-2, ćelijska linija duvana, upotrebljena je za produkciju novog tipa aspartatne proteinaze heljde - FeAPL1-His6. Potvrđena je očekivana pepstatin-A senzitivna enzimska aktivnost ovog proteina na pH 3,0. Lokalizacija proteina FeAPL1-His6 je određena imunocitohemijski i uporednom analizom Western blot transformisanih ćelija BY-2 i njihovih protoplasta. Ovim metodama je utvrđeno da ispitivani protein ima ekstracelularnu lokalizaciju u ćelijskom zidu. Na osnovu dobijenih rezultata razmatrana je potencijalna funkcija proteaze FeAPL1., The recombinant aspartic proteinase-like protein (FeAPL1-His6) was overexpressed in the tobacco BY-2 cell line and the expected pepstatin A-sensitive enzymatic activity was confirmed at pH 3.0. Immunocytochemistry and protein gel blot analysis of the transformed BY-2 cells and their protoplasts showed extracellular localization of rFeAPL1-His6 in the cell wall. Based on the obtained results, potential functions of FeAPL1 are discussed.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana, Cell wall localization of the aspartic proteinase from buckwheat (FeAPL1) over-expressed in tobacco BY-2 cells",
pages = "1236-1229",
number = "9",
volume = "76",
doi = "10.2298/JSC110120106M"
}

Milisavljević, M., Timotijević, G., Nikolić, D., Samardžić, J.,& Maksimović, V. R.. (2011). Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 76(9), 1229-1236.
https://doi.org/10.2298/JSC110120106M

Milisavljević M, Timotijević G, Nikolić D, Samardžić J, Maksimović VR. Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana. in Journal of the Serbian Chemical Society. 2011;76(9):1229-1236.
doi:10.2298/JSC110120106M .

Milisavljević, Mira, Timotijević, Gordana, Nikolić, Dragana, Samardžić, Jelena, Maksimović, Vesna R., "Ćelijska lokalizacija novog tipa aspartatne proteinaze heljde (FeAPL1) u transformisanim BY-2 ćelijama duvana" in Journal of the Serbian Chemical Society, 76, no. 9 (2011):1229-1236,
https://doi.org/10.2298/JSC110120106M . .

TY  - JOUR
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Dragana
AU  - Jovanović, Živko
AU  - Samardžić, Jelena
AU  - Radović, Svetlana R.
AU  - Maksimović, Vesna R.
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/528
AB  - Oxidative stress and antioxidative defense system activity were studied in buckwheat leaves after complete submergence and re-aeration. The levels of H2O2 and lipid peroxidation were found to be significantly higher in stressed than in untreated buckwheat leaves. Enzymes catalyzing the degradation of H2O2 and peroxides were shown to participate actively, whereas superoxide dismutase did not take part in the buckwheat leaf response to flooding stress. The most prominent increase in antioxidative enzyme activities was noticed upon return to air, when the strongest oxidative stress occurred and the need for antioxidative defense was the greatest.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence
EP  - 405
IS  - 2
SP  - 399
VL  - 63
DO  - 10.2298/ABS1102399S
ER  - 

@article{
author = "Stanisavljević, Nemanja and Nikolić, Dragana and Jovanović, Živko and Samardžić, Jelena and Radović, Svetlana R. and Maksimović, Vesna R.",
year = "2011",
abstract = "Oxidative stress and antioxidative defense system activity were studied in buckwheat leaves after complete submergence and re-aeration. The levels of H2O2 and lipid peroxidation were found to be significantly higher in stressed than in untreated buckwheat leaves. Enzymes catalyzing the degradation of H2O2 and peroxides were shown to participate actively, whereas superoxide dismutase did not take part in the buckwheat leaf response to flooding stress. The most prominent increase in antioxidative enzyme activities was noticed upon return to air, when the strongest oxidative stress occurred and the need for antioxidative defense was the greatest.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence",
pages = "405-399",
number = "2",
volume = "63",
doi = "10.2298/ABS1102399S"
}

Stanisavljević, N., Nikolić, D., Jovanović, Ž., Samardžić, J., Radović, S. R.,& Maksimović, V. R.. (2011). Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(2), 399-405.
https://doi.org/10.2298/ABS1102399S

Stanisavljević N, Nikolić D, Jovanović Ž, Samardžić J, Radović SR, Maksimović VR. Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence. in Archives of Biological Sciences. 2011;63(2):399-405.
doi:10.2298/ABS1102399S .

Stanisavljević, Nemanja, Nikolić, Dragana, Jovanović, Živko, Samardžić, Jelena, Radović, Svetlana R., Maksimović, Vesna R., "Antioxidative enzymes in the response of buckwheat (Fagopyrum esculentum Moench) to complete submergence" in Archives of Biological Sciences, 63, no. 2 (2011):399-405,
https://doi.org/10.2298/ABS1102399S . .

TY  - JOUR
AU  - Bratić, Ana M.
AU  - Majić, Dragana 
AU  - Samardžić, Jelena
AU  - Kragl, M.W.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/428
AB  - Ekspresija gena je regulisana posredstvom promotora-DNK sekvenci uzvodno od kodirajućeg regiona. Promotori svojom sekvencom određuju mesta vezivanja transkripcionih faktora, regulatora i RNK polimeraze. Strukturna i funkcionalna analiza promotora neophodna je za razumevanje mehanizama genske ekspresije. Cilj ovog rada je razvijanje brzog, efikasnog i pouzdanog sistema za testiranje bazalne promotorske aktivnosti kao i otkrivanje sekvenci polen-specifičnih elemenata promotora. U ovom radu je testirana funkcionalnost promotora za metalotionein heljde, posredstvom histohemijskog GUS-eseja u dva tranzijentna sistema za ekspresiju: BY2 ćelijama i polenovim zrnima. U oba sistema je uočena izražena aktivnost ispitivanog promotora.
AB  - Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena
T1  - Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes
EP  - 62
IS  - 1
SP  - 57
VL  - 62
DO  - 10.2298/ABS1001057B
ER  - 

@article{
author = "Bratić, Ana M. and Majić, Dragana  and Samardžić, Jelena and Kragl, M.W. and Maksimović, Vesna R.",
year = "2010",
abstract = "Ekspresija gena je regulisana posredstvom promotora-DNK sekvenci uzvodno od kodirajućeg regiona. Promotori svojom sekvencom određuju mesta vezivanja transkripcionih faktora, regulatora i RNK polimeraze. Strukturna i funkcionalna analiza promotora neophodna je za razumevanje mehanizama genske ekspresije. Cilj ovog rada je razvijanje brzog, efikasnog i pouzdanog sistema za testiranje bazalne promotorske aktivnosti kao i otkrivanje sekvenci polen-specifičnih elemenata promotora. U ovom radu je testirana funkcionalnost promotora za metalotionein heljde, posredstvom histohemijskog GUS-eseja u dva tranzijentna sistema za ekspresiju: BY2 ćelijama i polenovim zrnima. U oba sistema je uočena izražena aktivnost ispitivanog promotora., Gene expression is mediated by DNA sequences directly upstream from the coding sequences, recruited transcription factors and RNA polymerase in a spatially-defined manner. Understanding promoter strength and regulation would enhance our understanding of gene expression. The goal of this study was to develop a fast, efficient and reliable method for testing basal promoter activity and identifying core sequences within its pollen specific elements. In this paper we examined the functionality of buckwheat metallothionein promoter by a histochemical GUS assay in two transient expression systems: BY2 cells and pollen grains. Strong promoter activity was observed in both systems.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena, Transient expression in tobacco Bright Yellow 2 cells and pollen grains: A fast, efficient and reliable system for functional promoter analysis of plant genes",
pages = "62-57",
number = "1",
volume = "62",
doi = "10.2298/ABS1001057B"
}

Bratić, A. M., Majić, D., Samardžić, J., Kragl, M.W.,& Maksimović, V. R.. (2010). Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 57-62.
https://doi.org/10.2298/ABS1001057B

Bratić AM, Majić D, Samardžić J, Kragl M, Maksimović VR. Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena. in Archives of Biological Sciences. 2010;62(1):57-62.
doi:10.2298/ABS1001057B .

Bratić, Ana M., Majić, Dragana , Samardžić, Jelena, Kragl, M.W., Maksimović, Vesna R., "Tranzijentna ekspresija u Bright Yellow 2 ćelijama duvana i polenovim zrnima kao brz, efikasan i pouzdan sistem za funkcionalnu analizu promotora biljnih gena" in Archives of Biological Sciences, 62, no. 1 (2010):57-62,
https://doi.org/10.2298/ABS1001057B . .

TY  - JOUR
AU  - Nikolić, Dragana
AU  - Samardžić, Jelena
AU  - Bratić, Ana M.
AU  - Radin, Ivan P.
AU  - Gavrilović, Srdjan P.
AU  - Rausch, Thomas
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/417
AB  - The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments
EP  - 3494
IS  - 6
SP  - 3488
VL  - 58
DO  - 10.1021/jf904483a
ER  - 

@article{
author = "Nikolić, Dragana and Samardžić, Jelena and Bratić, Ana M. and Radin, Ivan P. and Gavrilović, Srdjan P. and Rausch, Thomas and Maksimović, Vesna R.",
year = "2010",
abstract = "The protective role in vivo of buckwheat metallothionein type 3 (FeMT3) during metal stress and the responsiveness of its promoter to metal ions were examined. Increased tolerance to heavy metals of FeMT3 producing Escherichia coli and cup1(Delta) yeast cells was detected. The defensive ability of buckwheat MT3 during Cd and Cu stresses was also demonstrated in Nicotiana debneyii leaves transiently expressing FeMT3. In contrast to phytochelatins, the cytoplasmatic localization of FeMT3 was not altered under heavy metal stress. Functional analysis of the corresponding promoter region revealed extremely high inducibility upon Cu2+ and Cd2+ treatments. The confirmed defense ability of FeMT3 protein in vivo and the great responsiveness of its promoter during heavy metal exposure make this gene a suitable candidate for biotechnological applications.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments",
pages = "3494-3488",
number = "6",
volume = "58",
doi = "10.1021/jf904483a"
}

Nikolić, D., Samardžić, J., Bratić, A. M., Radin, I. P., Gavrilović, S. P., Rausch, T.,& Maksimović, V. R.. (2010). Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 58(6), 3488-3494.
https://doi.org/10.1021/jf904483a

Nikolić D, Samardžić J, Bratić AM, Radin IP, Gavrilović SP, Rausch T, Maksimović VR. Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments. in Journal of Agricultural and Food Chemistry. 2010;58(6):3488-3494.
doi:10.1021/jf904483a .

Nikolić, Dragana, Samardžić, Jelena, Bratić, Ana M., Radin, Ivan P., Gavrilović, Srdjan P., Rausch, Thomas, Maksimović, Vesna R., "Buckwheat (Fagopyrum esculentum Moench) FeMT3 Gene in Heavy Metal Stress: Protective Role of the Protein and Inducibility of the Promoter Region under Cu2+ and Cd2+ Treatments" in Journal of Agricultural and Food Chemistry, 58, no. 6 (2010):3488-3494,
https://doi.org/10.1021/jf904483a . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Radović, Svetlana R.
AU  - Konstantinović, Miroslav M.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/409
AB  - The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat
EP  - 68
IS  - 1
SP  - 61
VL  - 167
DO  - 10.1016/j.jplph.2009.06.017
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Radović, Svetlana R. and Konstantinović, Miroslav M. and Maksimović, Vesna R.",
year = "2010",
abstract = "The aspartic protease (FeAP9) gene from buckwheat resembles the exon-intron structure characteristic for typical aspartic proteinases, including the presence of the leader intron in the 5'-UTR. RT PCR experiments and gel protein blot analysis indicated that FeAP9 was present in all analyzed organs: developing seeds, seedlings, flowers, leaves, roots and stems. Using Real-time PCR, we found that FeAP9 expression is upregulated in buckwheat leaves under the influence of different abiotic stresses, including dark, drought and UV-B light, as well as wounding and salicylic acid.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat",
pages = "68-61",
number = "1",
volume = "167",
doi = "10.1016/j.jplph.2009.06.017"
}

Timotijević, G., Milisavljević, M., Radović, S. R., Konstantinović, M. M.,& Maksimović, V. R.. (2010). Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 167(1), 61-68.
https://doi.org/10.1016/j.jplph.2009.06.017

Timotijević G, Milisavljević M, Radović SR, Konstantinović MM, Maksimović VR. Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat. in Journal of Plant Physiology. 2010;167(1):61-68.
doi:10.1016/j.jplph.2009.06.017 .

Timotijević, Gordana, Milisavljević, Mira, Radović, Svetlana R., Konstantinović, Miroslav M., Maksimović, Vesna R., "Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat" in Journal of Plant Physiology, 167, no. 1 (2010):61-68,
https://doi.org/10.1016/j.jplph.2009.06.017 . .

TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Miljuš-Đukić, Jovanka
AU  - Konstantinović, M.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/462
AB  - Kod biljaka cvetnica postoje genetički određeni sistemi self-inkompatibilnosti (SI), koji sprečavaju samooprašivanje i ukrštanje u srodstvu održavajući genetičku raznovrsnost vrsta. SI se javlja u dva oblika, kao gametofitna i sporofitna SI, koje se razlikuju u načinu određivanja SI fenotipa polena - kod GSI je SI fenotip polena određen polenovim sopstvenim haploidnim genomom, dok je kod SSI određen dipolidnim genotipom majke biljke. SSI se javlja kao homomorfna (jedan tip cveta u biljaka jedne vrste) i heteromorfna (dva ili tri tipa cveta u biljaka jedne vrste). Heteromorfna SSI je u poređenju sa homomorfnom SSI i GSI izuzetno malo proučena i za sada je upoznavanje na molekularnom nivou tek započelo. Kod heljde je prisutna distilna heteromorfna SSI, o kojoj je sakupljeno dosta podataka na fiziološkom nivou, ali o kojoj za sada nema molekularnih podataka. Na osnovu fiziološke sličnosti SI odgovora biljaka rodova Brassica i Prunus sa tram i pin morfom heljde, respektivno, zatim na osnovu toga što postoje dokazi da slični biohemijski mehanizmi leže u osnovi različitih SI odgovora i na osnovu toga što i evolutivno udaljene SI vrste mogu posedovati iste ili slične predačke SI gene, mi smo odlučili da ispitamo prisustvo ortologih gena uključenih u SI odgovore Brassica i Prunus u genomu heljde. Upotrebom izrođenih prajmera dizajniranih na osnovu evolutivno očuvanih regiona SRK, SLG, SP11 i MLPK sekvenci Brassica rapa, kao i S-RNaza i SFB gena roda Prunus, dostupnih u NCBI bazi podataka, ispitano je prisustvo ortologa ovih gena u genomu heljde. Takođe je prisustvo S-RNaza ispitano u proteinskim izolatima neoprašenih i kompatibilno i inkompatibilno oprašenih tučkova heljde oba morfa. Rezultati su pokazali da nema ortologa SRK, SLG, SP11, kao ni S-RNaza i SFB u genomu heljde, ali da postoji MLPK ortolog kod heljde. Izvedena aminokiselinska sekvenca pokazala je 80 % sličnosti sa MLPKf2 sekvencom Brassica rapa i APK1A Arabidopsis thaliana, potvrđujući da su u pitanju ortolozi koji bi mogli da imaju i sličnu ulogu. Naš sledeći korak je dobijanje cele nukleotidne sekvence MLPK heljde uz is- pitivanje postojanja alternativnih mesta iskrajanja i određivanje nivoa ekspresije po tkivima, kao i ispitivanje moguće uloge u SI odgovoru heljde. Ovi odgovori omogućiće bolje upoznavanje heteromorfnih SSI sistema koji su još uvek u svojoj najranijoj fazi istraživanja i obezbediće podatke nužne za uvid u evoluciju SI sistema biljaka cvetnica. Najzad, rasvetljavanjem SSI sistema heljde, koja se koristi u ishrani, biće moguće genetički kontrolisati ukrštanje heljde i dobijanje linija sa željenim hranljivim i/ili fiziološkim osobinama.
AB  - Self-incompatibility (SI) systems, gamethophytic (GSI) and sporophytic (SSI), prevent self-pollination in angiosperms. Buckwheat displays heteromorphic SSI, with pollination allowed only between different flower morphs - thrum and pin. The physiology of thrum and pin morph SI responses are entirely different, resembling homomorphic Brassica SSI and Prunus GSI responses, respectively. Considering angiosperm species may share ancestral SI genes, we examined the presence of Brassica and Prunus SI-involved gene orthologs in the buckwheat genome. We did not find evidence of SRK, SLG and SP11 Brassica or S-RNase and SFB Prunus orthologs in the buckwheat genome, but we found a Brassica MLPK ortholog. We report the partial nucleotide sequence of the buckwheat MLPK and discuss the possible implications of this finding.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Nova genska sekvenca identifikovana kod heljde - MLPK sa mogućom ulogom u SSI odgovoru
T1  - A search of Brassica SI-involved orthologs in buckwheat leads to novel buckwheat sequence identification: MLPK possibly involved in SI response
EP  - 321
IS  - 2
SP  - 315
VL  - 62
DO  - 10.2298/ABS1002315B
ER  - 

@article{
author = "Banović Đeri, Bojana and Miljuš-Đukić, Jovanka and Konstantinović, M. and Maksimović, Vesna R.",
year = "2010",
abstract = "Kod biljaka cvetnica postoje genetički određeni sistemi self-inkompatibilnosti (SI), koji sprečavaju samooprašivanje i ukrštanje u srodstvu održavajući genetičku raznovrsnost vrsta. SI se javlja u dva oblika, kao gametofitna i sporofitna SI, koje se razlikuju u načinu određivanja SI fenotipa polena - kod GSI je SI fenotip polena određen polenovim sopstvenim haploidnim genomom, dok je kod SSI određen dipolidnim genotipom majke biljke. SSI se javlja kao homomorfna (jedan tip cveta u biljaka jedne vrste) i heteromorfna (dva ili tri tipa cveta u biljaka jedne vrste). Heteromorfna SSI je u poređenju sa homomorfnom SSI i GSI izuzetno malo proučena i za sada je upoznavanje na molekularnom nivou tek započelo. Kod heljde je prisutna distilna heteromorfna SSI, o kojoj je sakupljeno dosta podataka na fiziološkom nivou, ali o kojoj za sada nema molekularnih podataka. Na osnovu fiziološke sličnosti SI odgovora biljaka rodova Brassica i Prunus sa tram i pin morfom heljde, respektivno, zatim na osnovu toga što postoje dokazi da slični biohemijski mehanizmi leže u osnovi različitih SI odgovora i na osnovu toga što i evolutivno udaljene SI vrste mogu posedovati iste ili slične predačke SI gene, mi smo odlučili da ispitamo prisustvo ortologih gena uključenih u SI odgovore Brassica i Prunus u genomu heljde. Upotrebom izrođenih prajmera dizajniranih na osnovu evolutivno očuvanih regiona SRK, SLG, SP11 i MLPK sekvenci Brassica rapa, kao i S-RNaza i SFB gena roda Prunus, dostupnih u NCBI bazi podataka, ispitano je prisustvo ortologa ovih gena u genomu heljde. Takođe je prisustvo S-RNaza ispitano u proteinskim izolatima neoprašenih i kompatibilno i inkompatibilno oprašenih tučkova heljde oba morfa. Rezultati su pokazali da nema ortologa SRK, SLG, SP11, kao ni S-RNaza i SFB u genomu heljde, ali da postoji MLPK ortolog kod heljde. Izvedena aminokiselinska sekvenca pokazala je 80 % sličnosti sa MLPKf2 sekvencom Brassica rapa i APK1A Arabidopsis thaliana, potvrđujući da su u pitanju ortolozi koji bi mogli da imaju i sličnu ulogu. Naš sledeći korak je dobijanje cele nukleotidne sekvence MLPK heljde uz is- pitivanje postojanja alternativnih mesta iskrajanja i određivanje nivoa ekspresije po tkivima, kao i ispitivanje moguće uloge u SI odgovoru heljde. Ovi odgovori omogućiće bolje upoznavanje heteromorfnih SSI sistema koji su još uvek u svojoj najranijoj fazi istraživanja i obezbediće podatke nužne za uvid u evoluciju SI sistema biljaka cvetnica. Najzad, rasvetljavanjem SSI sistema heljde, koja se koristi u ishrani, biće moguće genetički kontrolisati ukrštanje heljde i dobijanje linija sa željenim hranljivim i/ili fiziološkim osobinama., Self-incompatibility (SI) systems, gamethophytic (GSI) and sporophytic (SSI), prevent self-pollination in angiosperms. Buckwheat displays heteromorphic SSI, with pollination allowed only between different flower morphs - thrum and pin. The physiology of thrum and pin morph SI responses are entirely different, resembling homomorphic Brassica SSI and Prunus GSI responses, respectively. Considering angiosperm species may share ancestral SI genes, we examined the presence of Brassica and Prunus SI-involved gene orthologs in the buckwheat genome. We did not find evidence of SRK, SLG and SP11 Brassica or S-RNase and SFB Prunus orthologs in the buckwheat genome, but we found a Brassica MLPK ortholog. We report the partial nucleotide sequence of the buckwheat MLPK and discuss the possible implications of this finding.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Nova genska sekvenca identifikovana kod heljde - MLPK sa mogućom ulogom u SSI odgovoru, A search of Brassica SI-involved orthologs in buckwheat leads to novel buckwheat sequence identification: MLPK possibly involved in SI response",
pages = "321-315",
number = "2",
volume = "62",
doi = "10.2298/ABS1002315B"
}

Banović Đeri, B., Miljuš-Đukić, J., Konstantinović, M.,& Maksimović, V. R.. (2010). Nova genska sekvenca identifikovana kod heljde - MLPK sa mogućom ulogom u SSI odgovoru. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 315-321.
https://doi.org/10.2298/ABS1002315B

Banović Đeri B, Miljuš-Đukić J, Konstantinović M, Maksimović VR. Nova genska sekvenca identifikovana kod heljde - MLPK sa mogućom ulogom u SSI odgovoru. in Archives of Biological Sciences. 2010;62(2):315-321.
doi:10.2298/ABS1002315B .

Banović Đeri, Bojana, Miljuš-Đukić, Jovanka, Konstantinović, M., Maksimović, Vesna R., "Nova genska sekvenca identifikovana kod heljde - MLPK sa mogućom ulogom u SSI odgovoru" in Archives of Biological Sciences, 62, no. 2 (2010):315-321,
https://doi.org/10.2298/ABS1002315B . .

TY  - JOUR
AU  - Samardžić, Jelena
AU  - Nikolić, Dragana
AU  - Timotijević, Gordana
AU  - Jovanović, Živko
AU  - Milisavljević, Mira
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/436
AB  - Metallothionein type 3 (MT3) expression has previously been detected in leaves fruits and developing somatic embryos in different plant species However specific tissular and cellular localization of MT3 transcripts have remained unidentified In this study in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements mesophyll and guard cells of leaves vascular tissue of roots and throughout the whole embryo Changes in FeMT3 mRNA levels in response to drought and oxidative stress as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)
EP  - 1411
IS  - 16
SP  - 1407
VL  - 167
DO  - 10.1016/j.jplph.2010.05.016
ER  - 

@article{
author = "Samardžić, Jelena and Nikolić, Dragana and Timotijević, Gordana and Jovanović, Živko and Milisavljević, Mira and Maksimović, Vesna R.",
year = "2010",
abstract = "Metallothionein type 3 (MT3) expression has previously been detected in leaves fruits and developing somatic embryos in different plant species However specific tissular and cellular localization of MT3 transcripts have remained unidentified In this study in situ RNA-RNA analysis revealed buckwheat metallothionein type 3 (FeMT3) transcript localization in vascular elements mesophyll and guard cells of leaves vascular tissue of roots and throughout the whole embryo Changes in FeMT3 mRNA levels in response to drought and oxidative stress as well as ROS scavenging abilities of the FeMT3 protein in yeast were also detected indicating possible involvement of FeMT3 in stress defense and ROS related cellular processes",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)",
pages = "1411-1407",
number = "16",
volume = "167",
doi = "10.1016/j.jplph.2010.05.016"
}

Samardžić, J., Nikolić, D., Timotijević, G., Jovanović, Ž., Milisavljević, M.,& Maksimović, V. R.. (2010). Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum). in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 167(16), 1407-1411.
https://doi.org/10.1016/j.jplph.2010.05.016

Samardžić J, Nikolić D, Timotijević G, Jovanović Ž, Milisavljević M, Maksimović VR. Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum). in Journal of Plant Physiology. 2010;167(16):1407-1411.
doi:10.1016/j.jplph.2010.05.016 .

Samardžić, Jelena, Nikolić, Dragana, Timotijević, Gordana, Jovanović, Živko, Milisavljević, Mira, Maksimović, Vesna R., "Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)" in Journal of Plant Physiology, 167, no. 16 (2010):1407-1411,
https://doi.org/10.1016/j.jplph.2010.05.016 . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Milisavljević, Mira
AU  - Radović, Svetlana R.
AU  - Konstantinović, M.M.
AU  - Maksimović, Vesna R.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/429
AB  - Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa.
AB  - Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench)
T1  - Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)
EP  - 151
IS  - 1
SP  - 143
VL  - 62
DO  - 10.2298/ABS1001143T
ER  - 

@article{
author = "Timotijević, Gordana and Milisavljević, Mira and Radović, Svetlana R. and Konstantinović, M.M. and Maksimović, Vesna R.",
year = "2010",
abstract = "Gen za aspartičnu proteinazu (FeAP12) je izolovan iz eDNA biblioteke semena heljde u razviću. Analiza izvedene amino kiselinske sekvence FeAP12 gena ukazuje na njenu visoku homologiju sa ostalim tipičnim biljnim aspartičnim proteinazama (AP) koje se odlikuju prisustvom biljno specifičnog inserta (plant specific insert PSI), jedinstvenog među AP. Pokazano je da gen FeAP12 nije eksprimiran u listu, korenu, stablu i cvetu, već da je iRNA za FeAP12 prisutna samo u semenu. Najveći nivo ekspresije ovog gena je uočen u ranim fazama razvića semena, što ukazuje na njegovu moguću ulogu u degradaciji nucelusa., Aspartic proteinase gene (FeAP12) has been isolated from the cDNA library of developing buckwheat seeds. Analysis of its deduced amino acid sequence showed that it resembled the structure and shared high homology with typical plant aspartic proteinases (AP) characterized by the presence of a plant-specific insert (PSI), unique among APs. It was shown that FeAP12 mRNA was not present in the leaves, roots, steam and flowers, but was seed-specifically expressed. Moreover, the highest levels of FeAP12 expression were observed in the early stages of seed development, therefore suggesting its potential role in nucellar degradation.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench), Seed-specific aspartic proteinase FeAP12 from buckwheat (Fagopyrum esculentum Moench)",
pages = "151-143",
number = "1",
volume = "62",
doi = "10.2298/ABS1001143T"
}

Timotijević, G., Milisavljević, M., Radović, S. R., Konstantinović, M.M.,& Maksimović, V. R.. (2010). Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(1), 143-151.
https://doi.org/10.2298/ABS1001143T

Timotijević G, Milisavljević M, Radović SR, Konstantinović M, Maksimović VR. Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences. 2010;62(1):143-151.
doi:10.2298/ABS1001143T .

Timotijević, Gordana, Milisavljević, Mira, Radović, Svetlana R., Konstantinović, M.M., Maksimović, Vesna R., "Aspartična proteinaza (FeAP12) je specifično eksprimirana u semenu heljde (Fagopyrum esculentum Moench)" in Archives of Biological Sciences, 62, no. 1 (2010):143-151,
https://doi.org/10.2298/ABS1001143T . .

TY  - JOUR
AU  - Banović Đeri, Bojana
AU  - Surbanovski, Nada
AU  - Konstantinović, Miroslav
AU  - Maksimović, Vesna R.
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/362
AB  - In order to investigate the S-RNase allele structure of a Prunus webbii population from the Montenegrin region of the Balkans, we analyzed 10 Prunus webbii accessions. We detected 10 different S-RNase allelic variants and obtained the nucleotide sequences for six S-RNases. The BLAST analysis showed that these six sequences were new Prunus webbii S-RNase alleles. It also revealed that one of sequenced alleles, S(9)-RNase, coded for an amino acid sequence identical to that for Prunus dulcis S(14)-RNase, except for a single conservative amino acid replacement in the signal peptide region. Another, S(3)-RNase, was shown to differ by only three amino acid residues from Prunus salicina Se-RNase. The allele S(7)-RNase was found to be inactive by stylar protein isoelectric focusing followed by RNase-specific staining, but the reason for the inactivity was not at the coding sequence level. Further, in five of the 10 analyzed accessions, we detected the presence of one active basic RNase (marked PW(1)) that did not amplify with S-RNase-specific DNA primers. However, it was amplified with primers designed from the PA1 RNase nucleotide sequence (basic "non-S RNase" of Prunus avium) and the obtained sequence showed high homology (80%) with the PA1 allele. Although homologs of PA1 "non-S RNases" have been reported in four other Prunus species, this is the first recorded homolog in Prunus webbii. The evolutionary implications of the data are discussed.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes
EP  - 402
IS  - 4
SP  - 395
VL  - 166
DO  - 10.1016/j.jplph.2008.06.009
ER  - 

@article{
author = "Banović Đeri, Bojana and Surbanovski, Nada and Konstantinović, Miroslav and Maksimović, Vesna R.",
year = "2009",
abstract = "In order to investigate the S-RNase allele structure of a Prunus webbii population from the Montenegrin region of the Balkans, we analyzed 10 Prunus webbii accessions. We detected 10 different S-RNase allelic variants and obtained the nucleotide sequences for six S-RNases. The BLAST analysis showed that these six sequences were new Prunus webbii S-RNase alleles. It also revealed that one of sequenced alleles, S(9)-RNase, coded for an amino acid sequence identical to that for Prunus dulcis S(14)-RNase, except for a single conservative amino acid replacement in the signal peptide region. Another, S(3)-RNase, was shown to differ by only three amino acid residues from Prunus salicina Se-RNase. The allele S(7)-RNase was found to be inactive by stylar protein isoelectric focusing followed by RNase-specific staining, but the reason for the inactivity was not at the coding sequence level. Further, in five of the 10 analyzed accessions, we detected the presence of one active basic RNase (marked PW(1)) that did not amplify with S-RNase-specific DNA primers. However, it was amplified with primers designed from the PA1 RNase nucleotide sequence (basic "non-S RNase" of Prunus avium) and the obtained sequence showed high homology (80%) with the PA1 allele. Although homologs of PA1 "non-S RNases" have been reported in four other Prunus species, this is the first recorded homolog in Prunus webbii. The evolutionary implications of the data are discussed.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes",
pages = "402-395",
number = "4",
volume = "166",
doi = "10.1016/j.jplph.2008.06.009"
}

Banović Đeri, B., Surbanovski, N., Konstantinović, M.,& Maksimović, V. R.. (2009). Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 166(4), 395-402.
https://doi.org/10.1016/j.jplph.2008.06.009

Banović Đeri B, Surbanovski N, Konstantinović M, Maksimović VR. Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes. in Journal of Plant Physiology. 2009;166(4):395-402.
doi:10.1016/j.jplph.2008.06.009 .

Banović Đeri, Bojana, Surbanovski, Nada, Konstantinović, Miroslav, Maksimović, Vesna R., "Basic RNases of wild almond (Prunus webbii): Cloning and characterization of six new S-RNase and one "non-S RNase" genes" in Journal of Plant Physiology, 166, no. 4 (2009):395-402,
https://doi.org/10.1016/j.jplph.2008.06.009 . .

TY  - JOUR
AU  - Bratić, Ana M.
AU  - Nikolić, Dragana
AU  - Samardžić, Jelena
AU  - Maksimović, Vesna R.
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/355
AB  - To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different factors.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli
EP  - 1000
IS  - 9
SP  - 996
VL  - 166
DO  - 10.1016/j.jplph.2008.12.002
ER  - 

@article{
author = "Bratić, Ana M. and Nikolić, Dragana and Samardžić, Jelena and Maksimović, Vesna R.",
year = "2009",
abstract = "To shed light on expression regulation of the metallothionein gene from buckwheat (FeMT3), functional promoter analysis was performed with a complete 5' regulatory region and two deletion variants, employing stably transformed tobacco plants. Histochemical GUS assay of transgenic tobacco lines showed the strongest. signals in vascular elements of leaves and in pollen grains, while somewhat weaker staining was observed in the roots of mature plants. This tissue specificity pattern implies a possible function of buckwheat MT3 in those tissues. Quantitative GUS assay showed strong up-regulation of all three promoter constructs (proportional to the length of the regulatory region) in leaves submerged in liquid MS medium containing sucrose, after a prolonged time period. This represented a complex stress situation composed of several synergistically related stress stimuli. These findings suggest: complex transcriptional regulation of FeMT3, requiring interactions among a number of different factors.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli",
pages = "1000-996",
number = "9",
volume = "166",
doi = "10.1016/j.jplph.2008.12.002"
}

Bratić, A. M., Nikolić, D., Samardžić, J.,& Maksimović, V. R.. (2009). Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 166(9), 996-1000.
https://doi.org/10.1016/j.jplph.2008.12.002

Bratić AM, Nikolić D, Samardžić J, Maksimović VR. Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli. in Journal of Plant Physiology. 2009;166(9):996-1000.
doi:10.1016/j.jplph.2008.12.002 .

Bratić, Ana M., Nikolić, Dragana, Samardžić, Jelena, Maksimović, Vesna R., "Functional analysis of the buckwheat metallothionein promoter: Tissue specificity pattern and up-regulation under complex stress stimuli" in Journal of Plant Physiology, 166, no. 9 (2009):996-1000,
https://doi.org/10.1016/j.jplph.2008.12.002 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Papić, Dražen R.
AU  - Timotijević, Gordana
AU  - Maksimović, Vesna R.
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/347
AB  - U ovom radu predstavljena je ekspresija rekombinantne atipične aspartatne proteinaze heljde (Fagopyrum esculentum) bogate cisteinom, gde su testirana različita ekspresiona svojstva pet sojeva E. coli. Takođe je analiziran i uticaj fuzionih partnera (His6 i MBP) na efikasnost ekspresije. U slučaju His6-FeAPL1, dobijena je velika količina nerastvornog proteina, smeštenog u inkluzionim telima. S druge strane, MBP-FeAPL1 je bio lokalizovan i u citoplazmi i u inkluzionim telima u oba upotrebljena soja E. coli (BL21 i Rosetta-gami). Međutim, samo za rekombinantni protein proizveden u soju Rosetta-gami, dokazana je proteolitička aktivnost na supstratu BSA, pri pH 3,0. Rezultati su takođe ukazali da FeAPL1 sadrži PRO segment, čije je odstranjivanje neophodno za njegovu proteolitičku aktivnost. Aktivnost FeAPL1, pokazana samo u soju Rosetta-gami, gde je moguće formiranje disulfidnih veza, ukazuje na značaj 12 cisteina u uspostavljanju pravilne strukture koja omogućava funkcionalnost enzima.
AB  - Herein, the expression of recombinant cysteine-rich atypical buckwheat (Fagopyrum esculentum) aspartic protease (FeAPL1) in five Escherichia coli strains differing in their expression capabilities is presented. It was shown that the expression success depended highly on the choice of FeAPL1 fusion partner. His6-FeAPL1 was produced in large quantities as an insoluble protein localized in inclusion bodies. On the other hand, MBP-FeAPL1 was localized in both the cytoplasm and inclusion bodies in BL21 and Rosetta-gami strains. Only purified soluble MBP-FeAPL1 from Rosetta-gami cells showed proteolytic activity at pH 3.0 with BSA as the substrate. The results also indicated that FeAPL1 contained a PRO segment that had to be removed for the enzyme activity to appear. The activity of FeAPL1 produced in the Rosetta-gami strain, which enables disulfide bond formation, indicated the importance of the twelve cysteine residues for correct folding and functionality.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Uspešna proizvodnja rekombinantne aspartatne proteinaze heljde bogate cisteinom u E.coli
T1  - Successful production of recombinant buckwheat cysteine-rich aspartic protease in Escherichia coli
EP  - 618
IS  - 6
SP  - 607
VL  - 74
DO  - 10.2298/JSC0906607M
ER  - 

@article{
author = "Milisavljević, Mira and Papić, Dražen R. and Timotijević, Gordana and Maksimović, Vesna R.",
year = "2009",
abstract = "U ovom radu predstavljena je ekspresija rekombinantne atipične aspartatne proteinaze heljde (Fagopyrum esculentum) bogate cisteinom, gde su testirana različita ekspresiona svojstva pet sojeva E. coli. Takođe je analiziran i uticaj fuzionih partnera (His6 i MBP) na efikasnost ekspresije. U slučaju His6-FeAPL1, dobijena je velika količina nerastvornog proteina, smeštenog u inkluzionim telima. S druge strane, MBP-FeAPL1 je bio lokalizovan i u citoplazmi i u inkluzionim telima u oba upotrebljena soja E. coli (BL21 i Rosetta-gami). Međutim, samo za rekombinantni protein proizveden u soju Rosetta-gami, dokazana je proteolitička aktivnost na supstratu BSA, pri pH 3,0. Rezultati su takođe ukazali da FeAPL1 sadrži PRO segment, čije je odstranjivanje neophodno za njegovu proteolitičku aktivnost. Aktivnost FeAPL1, pokazana samo u soju Rosetta-gami, gde je moguće formiranje disulfidnih veza, ukazuje na značaj 12 cisteina u uspostavljanju pravilne strukture koja omogućava funkcionalnost enzima., Herein, the expression of recombinant cysteine-rich atypical buckwheat (Fagopyrum esculentum) aspartic protease (FeAPL1) in five Escherichia coli strains differing in their expression capabilities is presented. It was shown that the expression success depended highly on the choice of FeAPL1 fusion partner. His6-FeAPL1 was produced in large quantities as an insoluble protein localized in inclusion bodies. On the other hand, MBP-FeAPL1 was localized in both the cytoplasm and inclusion bodies in BL21 and Rosetta-gami strains. Only purified soluble MBP-FeAPL1 from Rosetta-gami cells showed proteolytic activity at pH 3.0 with BSA as the substrate. The results also indicated that FeAPL1 contained a PRO segment that had to be removed for the enzyme activity to appear. The activity of FeAPL1 produced in the Rosetta-gami strain, which enables disulfide bond formation, indicated the importance of the twelve cysteine residues for correct folding and functionality.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Uspešna proizvodnja rekombinantne aspartatne proteinaze heljde bogate cisteinom u E.coli, Successful production of recombinant buckwheat cysteine-rich aspartic protease in Escherichia coli",
pages = "618-607",
number = "6",
volume = "74",
doi = "10.2298/JSC0906607M"
}

Milisavljević, M., Papić, D. R., Timotijević, G.,& Maksimović, V. R.. (2009). Uspešna proizvodnja rekombinantne aspartatne proteinaze heljde bogate cisteinom u E.coli. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 74(6), 607-618.
https://doi.org/10.2298/JSC0906607M

Milisavljević M, Papić DR, Timotijević G, Maksimović VR. Uspešna proizvodnja rekombinantne aspartatne proteinaze heljde bogate cisteinom u E.coli. in Journal of the Serbian Chemical Society. 2009;74(6):607-618.
doi:10.2298/JSC0906607M .

Milisavljević, Mira, Papić, Dražen R., Timotijević, Gordana, Maksimović, Vesna R., "Uspešna proizvodnja rekombinantne aspartatne proteinaze heljde bogate cisteinom u E.coli" in Journal of the Serbian Chemical Society, 74, no. 6 (2009):607-618,
https://doi.org/10.2298/JSC0906607M . .

TY  - JOUR
AU  - Majić, Dragana 
AU  - Samardžić, Jelena
AU  - Milisavljević, Mira
AU  - Krstić, A.M.
AU  - Maksimović, Vesna R.
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/307
AB  - Metalotioneini (MT) pripadaju velikoj grupi proteina male molekulske težine bogatih cisteinom, izražene sposobnosti za vezivanje jona metala, uključenih u procese održavanja homeostaze metalnih jona i detoksifikacije od teških metala. U radu je analizirana struktura dva transkripta gena za MT tipa 3 poreklom iz semena heljde u razviću. Razlike su nađene pre svega u okviru 3’- UTR sekvenci. Nakon analiza sekvenci urađena je analiza ekspresije tokom hipoksije korišćenjem tehnologije Real Tme RT-PCR.
AB  - Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the developing buckwheat seed cDNA library. Following sequence analyses, expression profiles during flooding stress were monitored by Real Time RT PCR technology.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Analiza strukture dva transkripta gena za metalotionein heljde i ispitivanje njihove ekspresije tokom hipoksije korišćenjem tehnologije Real Time RT-PCR
T1  - Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology
EP  - 82
IS  - 1
SP  - 77
VL  - 60
DO  - 10.2298/ABS0801077M
ER  - 

@article{
author = "Majić, Dragana  and Samardžić, Jelena and Milisavljević, Mira and Krstić, A.M. and Maksimović, Vesna R.",
year = "2008",
abstract = "Metalotioneini (MT) pripadaju velikoj grupi proteina male molekulske težine bogatih cisteinom, izražene sposobnosti za vezivanje jona metala, uključenih u procese održavanja homeostaze metalnih jona i detoksifikacije od teških metala. U radu je analizirana struktura dva transkripta gena za MT tipa 3 poreklom iz semena heljde u razviću. Razlike su nađene pre svega u okviru 3’- UTR sekvenci. Nakon analiza sekvenci urađena je analiza ekspresije tokom hipoksije korišćenjem tehnologije Real Tme RT-PCR., Metallothioneins (MTs) are an extensive and diverse family of small cysteine-rich proteins with metal-binding ability that are involved in metal homeostasis and detoxification. Two cDNA clones of the MT3 type, differing in 3’ UTRs, were isolated from the developing buckwheat seed cDNA library. Following sequence analyses, expression profiles during flooding stress were monitored by Real Time RT PCR technology.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Analiza strukture dva transkripta gena za metalotionein heljde i ispitivanje njihove ekspresije tokom hipoksije korišćenjem tehnologije Real Time RT-PCR, Two metallothionein gene family members in buckwheat: Expression analysis in flooding stress using Real Time RT-PCR technology",
pages = "82-77",
number = "1",
volume = "60",
doi = "10.2298/ABS0801077M"
}

Majić, D., Samardžić, J., Milisavljević, M., Krstić, A.M.,& Maksimović, V. R.. (2008). Analiza strukture dva transkripta gena za metalotionein heljde i ispitivanje njihove ekspresije tokom hipoksije korišćenjem tehnologije Real Time RT-PCR. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(1), 77-82.
https://doi.org/10.2298/ABS0801077M

Majić D, Samardžić J, Milisavljević M, Krstić A, Maksimović VR. Analiza strukture dva transkripta gena za metalotionein heljde i ispitivanje njihove ekspresije tokom hipoksije korišćenjem tehnologije Real Time RT-PCR. in Archives of Biological Sciences. 2008;60(1):77-82.
doi:10.2298/ABS0801077M .

Majić, Dragana , Samardžić, Jelena, Milisavljević, Mira, Krstić, A.M., Maksimović, Vesna R., "Analiza strukture dva transkripta gena za metalotionein heljde i ispitivanje njihove ekspresije tokom hipoksije korišćenjem tehnologije Real Time RT-PCR" in Archives of Biological Sciences, 60, no. 1 (2008):77-82,
https://doi.org/10.2298/ABS0801077M . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
AU  - Radović, Svettana R.
AU  - Konstantinović, Miroslav M.
AU  - Maksimović, Vesna R.
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/298
AB  - Two types of aspartic proteinase (AP) genes have been isolated from the cDNA library of developing buckwheat seeds. Analysis of their sequences showed that one of these, FeAP9, resembled the structure and shared high homology with the so-called typical plant APs characterized by the presence of a plant-specific insert (PSI), an element unique among APs. The other cDNA, FeAPL1, encoded an AP-like protein lacking that domain. Different expression profiles were observed for FeAP9 and FeAPL1. FeAPL1 mRNAs were restricted to the seeds only, whereas FeAP9 mRNAs were also present in the other plant tissues - Leaves, roots, and flowers. Higher Levels of FeAP9 were observed in senescent leaves compared with green leaves. The differential expression pattern of these two unique APs raises the interesting possibility that these proteinases have unique substrate specificity and may have different rotes in plant development and other physiological. processes.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis
EP  - 990
IS  - 9
SP  - 983
VL  - 165
DO  - 10.1016/j.jplph.2007.03.016
ER  - 

@article{
author = "Milisavljević, Mira and Timotijević, Gordana and Radović, Svettana R. and Konstantinović, Miroslav M. and Maksimović, Vesna R.",
year = "2008",
abstract = "Two types of aspartic proteinase (AP) genes have been isolated from the cDNA library of developing buckwheat seeds. Analysis of their sequences showed that one of these, FeAP9, resembled the structure and shared high homology with the so-called typical plant APs characterized by the presence of a plant-specific insert (PSI), an element unique among APs. The other cDNA, FeAPL1, encoded an AP-like protein lacking that domain. Different expression profiles were observed for FeAP9 and FeAPL1. FeAPL1 mRNAs were restricted to the seeds only, whereas FeAP9 mRNAs were also present in the other plant tissues - Leaves, roots, and flowers. Higher Levels of FeAP9 were observed in senescent leaves compared with green leaves. The differential expression pattern of these two unique APs raises the interesting possibility that these proteinases have unique substrate specificity and may have different rotes in plant development and other physiological. processes.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis",
pages = "990-983",
number = "9",
volume = "165",
doi = "10.1016/j.jplph.2007.03.016"
}

Milisavljević, M., Timotijević, G., Radović, S. R., Konstantinović, M. M.,& Maksimović, V. R.. (2008). Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 165(9), 983-990.
https://doi.org/10.1016/j.jplph.2007.03.016

Milisavljević M, Timotijević G, Radović SR, Konstantinović MM, Maksimović VR. Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis. in Journal of Plant Physiology. 2008;165(9):983-990.
doi:10.1016/j.jplph.2007.03.016 .

Milisavljević, Mira, Timotijević, Gordana, Radović, Svettana R., Konstantinović, Miroslav M., Maksimović, Vesna R., "Two types of aspartic proteinases from buckwheat seed - Gene structure and expression analysis" in Journal of Plant Physiology, 165, no. 9 (2008):983-990,
https://doi.org/10.1016/j.jplph.2007.03.016 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
AU  - Radović, Svetlana R.
AU  - Konstantinović, M.M.
AU  - Maksimović, Vesna R.
PY  - 2007
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/285
AB  - Iz biblioteke cDNK semena heljde u srednjoj fazi razvića izolovan je gen koji kodira novi tip aspartične proteinaze. Analizom sekvence ove cDNK (Fe-APL1) uočeno je odsustvo domena karakterističnog samo za biljne aspartične proteinaze, a analizom odgovrajućeg genomskog fragmenta da se radi o genu koji ne sadrži introne. Bioinformatičkim analizama genoma arabidopsisa je pokazano da je većina potencijalnih gena za aspartične proteinaze upravo sa ovim osobinama, iako je to eksperimentalno dokazano samo kod malog broja gena. Rezultati ovog rada daju doprinos u analizi raznovrsnosti unutar familije biljnih aspartičnih proteinaza. .
AB  - A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment was isolated. It is demonstrated that this gene does not contain introns. Since bioinformatics analysis of the Arabidopsis genome showed that most potential AP genes are intronless and PSI-less, it appears that "atypical" is an inappropriate word for that class of AP. Isolation of this specific buckwheat gene among the small group of those isolated from other plant species provides a new perspective on the diversity of AP family members in plants. .
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Izolovanje i analiza strukture gena koji kodira novi tip aspartične proteinaze semena heljde (Fagopyrum esculentum Moench)
T1  - Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)
EP  - 124
IS  - 2
SP  - 119
VL  - 59
DO  - 10.2298/ABS0702119M
ER  - 

@article{
author = "Milisavljević, Mira and Timotijević, Gordana and Radović, Svetlana R. and Konstantinović, M.M. and Maksimović, Vesna R.",
year = "2007",
abstract = "Iz biblioteke cDNK semena heljde u srednjoj fazi razvića izolovan je gen koji kodira novi tip aspartične proteinaze. Analizom sekvence ove cDNK (Fe-APL1) uočeno je odsustvo domena karakterističnog samo za biljne aspartične proteinaze, a analizom odgovrajućeg genomskog fragmenta da se radi o genu koji ne sadrži introne. Bioinformatičkim analizama genoma arabidopsisa je pokazano da je većina potencijalnih gena za aspartične proteinaze upravo sa ovim osobinama, iako je to eksperimentalno dokazano samo kod malog broja gena. Rezultati ovog rada daju doprinos u analizi raznovrsnosti unutar familije biljnih aspartičnih proteinaza. ., A novel type of aspartic proteinase gene was isolated from the cDNA library of developing buckwheat seeds. This cDNA, FeAPL1, encoded an AP-like protein lacking the plant-specific insert (PSI) domain characteristic of typical plant aspartic proteinases. In addition the corresponding genomic fragment was isolated. It is demonstrated that this gene does not contain introns. Since bioinformatics analysis of the Arabidopsis genome showed that most potential AP genes are intronless and PSI-less, it appears that "atypical" is an inappropriate word for that class of AP. Isolation of this specific buckwheat gene among the small group of those isolated from other plant species provides a new perspective on the diversity of AP family members in plants. .",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Izolovanje i analiza strukture gena koji kodira novi tip aspartične proteinaze semena heljde (Fagopyrum esculentum Moench), Isolation and structural analysis of a gene coding for a novel type of aspartic proteinase from buckwheat seed (Fagopyrum esculentum Moench)",
pages = "124-119",
number = "2",
volume = "59",
doi = "10.2298/ABS0702119M"
}

Milisavljević, M., Timotijević, G., Radović, S. R., Konstantinović, M.M.,& Maksimović, V. R.. (2007). Izolovanje i analiza strukture gena koji kodira novi tip aspartične proteinaze semena heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 59(2), 119-124.
https://doi.org/10.2298/ABS0702119M

Milisavljević M, Timotijević G, Radović SR, Konstantinović M, Maksimović VR. Izolovanje i analiza strukture gena koji kodira novi tip aspartične proteinaze semena heljde (Fagopyrum esculentum Moench). in Archives of Biological Sciences. 2007;59(2):119-124.
doi:10.2298/ABS0702119M .

Milisavljević, Mira, Timotijević, Gordana, Radović, Svetlana R., Konstantinović, M.M., Maksimović, Vesna R., "Izolovanje i analiza strukture gena koji kodira novi tip aspartične proteinaze semena heljde (Fagopyrum esculentum Moench)" in Archives of Biological Sciences, 59, no. 2 (2007):119-124,
https://doi.org/10.2298/ABS0702119M . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Radović, Svetlana R.
AU  - Maksimović, Vesna R.
PY  - 2006
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/260
AB  - Analizirane su aspartične proteinaze semena heljde. Upotrebom pepstatin A afinitetne hromatografije, iz zrelog semena izdvojene su tri forme aspartičnih proteinaza, od 47 kDa, 40 kDa i 28 kDa, dok je u ekstraktu nezrelog semena odsustvovala forma od 40 kDa. Protein od 47 kDa naknadno je razdvojen od ostalih formi kada je hromatografiji prethodila amonijum-sulfatna precipitacija. Pokazano je da tip proteolitičkog delovanja prečišćene forme enzima odgovara delovanju himozina, aspartične proteinaze animalnog porekla, čime bi se mogla objasniti njegova sposobnost da koaguliše mleko. Enzim je lokalizovan u membranskoj ćelijskoj frakciji.
AB  - Aspartic proteinases from buckwheat seeds are analyzed. Three forms of 47 kDa, 40 kDa and 28 kDa, were purified from mature buckwheat seeds, while two forms of 47 kDa and 28 kDa were detected in developing buckwheat seeds using pepstatin A affinity chromatography. A form of 47 kDa was selectively precipitated from other forms by ammonium sulfate precipitation. This enzyme resembles the chymosin-like pattern of proteolytic activity, as it was shown using BSA and k-casein as substrates, clarifying its ability for milk-clotting. The 47 kDa aspartic proteinase form is localized in the membrane fraction.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Aspartične proteinaze semena heljde (Fagopyrum esculentum moench) - prečišćavanje i svojstva enzima 47 kDa
T1  - Proteinases from buckwheat (Fagopyrum esculentum moench) seeds: Purification and properties of the 47 kDa enzyme
EP  - 177
IS  - 3
SP  - 171
VL  - 58
UR  - https://hdl.handle.net/21.15107/rcub_imagine_260
ER  - 

@article{
author = "Timotijević, Gordana and Radović, Svetlana R. and Maksimović, Vesna R.",
year = "2006",
abstract = "Analizirane su aspartične proteinaze semena heljde. Upotrebom pepstatin A afinitetne hromatografije, iz zrelog semena izdvojene su tri forme aspartičnih proteinaza, od 47 kDa, 40 kDa i 28 kDa, dok je u ekstraktu nezrelog semena odsustvovala forma od 40 kDa. Protein od 47 kDa naknadno je razdvojen od ostalih formi kada je hromatografiji prethodila amonijum-sulfatna precipitacija. Pokazano je da tip proteolitičkog delovanja prečišćene forme enzima odgovara delovanju himozina, aspartične proteinaze animalnog porekla, čime bi se mogla objasniti njegova sposobnost da koaguliše mleko. Enzim je lokalizovan u membranskoj ćelijskoj frakciji., Aspartic proteinases from buckwheat seeds are analyzed. Three forms of 47 kDa, 40 kDa and 28 kDa, were purified from mature buckwheat seeds, while two forms of 47 kDa and 28 kDa were detected in developing buckwheat seeds using pepstatin A affinity chromatography. A form of 47 kDa was selectively precipitated from other forms by ammonium sulfate precipitation. This enzyme resembles the chymosin-like pattern of proteolytic activity, as it was shown using BSA and k-casein as substrates, clarifying its ability for milk-clotting. The 47 kDa aspartic proteinase form is localized in the membrane fraction.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Aspartične proteinaze semena heljde (Fagopyrum esculentum moench) - prečišćavanje i svojstva enzima 47 kDa, Proteinases from buckwheat (Fagopyrum esculentum moench) seeds: Purification and properties of the 47 kDa enzyme",
pages = "177-171",
number = "3",
volume = "58",
url = "https://hdl.handle.net/21.15107/rcub_imagine_260"
}

Timotijević, G., Radović, S. R.,& Maksimović, V. R.. (2006). Aspartične proteinaze semena heljde (Fagopyrum esculentum moench) - prečišćavanje i svojstva enzima 47 kDa. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 58(3), 171-177.
https://hdl.handle.net/21.15107/rcub_imagine_260

Timotijević G, Radović SR, Maksimović VR. Aspartične proteinaze semena heljde (Fagopyrum esculentum moench) - prečišćavanje i svojstva enzima 47 kDa. in Archives of Biological Sciences. 2006;58(3):171-177.
https://hdl.handle.net/21.15107/rcub_imagine_260 .

Timotijević, Gordana, Radović, Svetlana R., Maksimović, Vesna R., "Aspartične proteinaze semena heljde (Fagopyrum esculentum moench) - prečišćavanje i svojstva enzima 47 kDa" in Archives of Biological Sciences, 58, no. 3 (2006):171-177,
https://hdl.handle.net/21.15107/rcub_imagine_260 .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Konstantinović, MM
AU  - Brkljačić, Jelena M.
AU  - Maksimović, Vesna R.
PY  - 2005
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/210
AB  - Using the modified rapid amplification of cDNA ends (5'-RACE) approach, a fragment containing the 955 bp long 5'-regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced, and the sequence was analyzed by computer prediction of cis-regulatory elements possibly involved in tissue-specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research but also as a useful tool for biotechnological application.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Isolation and computer analysis of the 5 '-regulatory region of the seed storage protein gene from buckwheat (Fagopyrum esculentum Moench)
EP  - 2080
IS  - 6
SP  - 2076
VL  - 53
DO  - 10.1021/jf048330g
ER  - 

@article{
author = "Milisavljević, Mira and Konstantinović, MM and Brkljačić, Jelena M. and Maksimović, Vesna R.",
year = "2005",
abstract = "Using the modified rapid amplification of cDNA ends (5'-RACE) approach, a fragment containing the 955 bp long 5'-regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced, and the sequence was analyzed by computer prediction of cis-regulatory elements possibly involved in tissue-specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research but also as a useful tool for biotechnological application.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Isolation and computer analysis of the 5 '-regulatory region of the seed storage protein gene from buckwheat (Fagopyrum esculentum Moench)",
pages = "2080-2076",
number = "6",
volume = "53",
doi = "10.1021/jf048330g"
}

Milisavljević, M., Konstantinović, M., Brkljačić, J. M.,& Maksimović, V. R.. (2005). Isolation and computer analysis of the 5 '-regulatory region of the seed storage protein gene from buckwheat (Fagopyrum esculentum Moench). in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 53(6), 2076-2080.
https://doi.org/10.1021/jf048330g

Milisavljević M, Konstantinović M, Brkljačić JM, Maksimović VR. Isolation and computer analysis of the 5 '-regulatory region of the seed storage protein gene from buckwheat (Fagopyrum esculentum Moench). in Journal of Agricultural and Food Chemistry. 2005;53(6):2076-2080.
doi:10.1021/jf048330g .

Milisavljević, Mira, Konstantinović, MM, Brkljačić, Jelena M., Maksimović, Vesna R., "Isolation and computer analysis of the 5 '-regulatory region of the seed storage protein gene from buckwheat (Fagopyrum esculentum Moench)" in Journal of Agricultural and Food Chemistry, 53, no. 6 (2005):2076-2080,
https://doi.org/10.1021/jf048330g . .

TY  - JOUR
AU  - Brkljačić, Jelena M.
AU  - Majić, Dragana
AU  - Miljuš-Đukić, Jovanka
AU  - Bratić, A
AU  - Konstantinović, MM
AU  - Milisavljević, Mira
AU  - Maksimović, Vesna R.
PY  - 2005
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/232
AB  - The buckwheat ( Fagopyrum esculentum Moench) metallothionein- like gene was cloned and its 59 regulatory region was examined. Computer analysis of this region using overlapping data from three different databases predicted the existence of regulatory sequences that could be involved in responses to different hormonal and external stimuli ( ERE, heat shock-HSE, light and stress-GT-1, I-box, GATA, G-box, metal-MRE) as well as the presence of putative binding sites for plant-specific transcription factors (Dof1, NtBBF1, Athb-1). Further investigation included analysis of the interactions of distal and proximal parts of the defined regulatory region and purified Dof1 Delta C domain of maize Dof1 and the HD-Zip-1 domain of Arabidopsis thaliana Athb-1 transcription factors as well as with buckwheat leaf nuclear extract. The identity of putative Dof1- and Athb-1-binding sites was confirmed in the proximal region. More important, we found that some proteins from buckwheat leaf nuclear extract compete for the Dof1- binding sites, indicating the presence of a similar protein type in that extract. We also showed that buckwheat leaf nuclear extract itself interacted with both proximal and distal promoter regions. Interaction with the proximal region resulted in the formation of a single HMW complex, while five separate complexes with the distal region were detected, indicating interaction with predicted G- and I-boxes, which are probably involved in light- and/or stress-regulated MT3 gene expression. The promoter ability of the cloned buckwheat MT gene regulatory region was characterized by measuring the activity of the GUS reporter gene in the leaves of transgenic tobacco. Buckwheat MT3 promoter activity was also analyzed for its response to stress produced by either hydrogen peroxide or UV treatment. We found that both treatments increased GUS activity.
PB  - Univ Chicago Press, Chicago
T2  - International Journal of Plant Sciences
T1  - The metallothionein-like gene from buckwheat: Structural and functional analysis of the promoter region
EP  - 900
IS  - 6
SP  - 889
VL  - 166
DO  - 10.1086/432690
ER  - 

@article{
author = "Brkljačić, Jelena M. and Majić, Dragana and Miljuš-Đukić, Jovanka and Bratić, A and Konstantinović, MM and Milisavljević, Mira and Maksimović, Vesna R.",
year = "2005",
abstract = "The buckwheat ( Fagopyrum esculentum Moench) metallothionein- like gene was cloned and its 59 regulatory region was examined. Computer analysis of this region using overlapping data from three different databases predicted the existence of regulatory sequences that could be involved in responses to different hormonal and external stimuli ( ERE, heat shock-HSE, light and stress-GT-1, I-box, GATA, G-box, metal-MRE) as well as the presence of putative binding sites for plant-specific transcription factors (Dof1, NtBBF1, Athb-1). Further investigation included analysis of the interactions of distal and proximal parts of the defined regulatory region and purified Dof1 Delta C domain of maize Dof1 and the HD-Zip-1 domain of Arabidopsis thaliana Athb-1 transcription factors as well as with buckwheat leaf nuclear extract. The identity of putative Dof1- and Athb-1-binding sites was confirmed in the proximal region. More important, we found that some proteins from buckwheat leaf nuclear extract compete for the Dof1- binding sites, indicating the presence of a similar protein type in that extract. We also showed that buckwheat leaf nuclear extract itself interacted with both proximal and distal promoter regions. Interaction with the proximal region resulted in the formation of a single HMW complex, while five separate complexes with the distal region were detected, indicating interaction with predicted G- and I-boxes, which are probably involved in light- and/or stress-regulated MT3 gene expression. The promoter ability of the cloned buckwheat MT gene regulatory region was characterized by measuring the activity of the GUS reporter gene in the leaves of transgenic tobacco. Buckwheat MT3 promoter activity was also analyzed for its response to stress produced by either hydrogen peroxide or UV treatment. We found that both treatments increased GUS activity.",
publisher = "Univ Chicago Press, Chicago",
journal = "International Journal of Plant Sciences",
title = "The metallothionein-like gene from buckwheat: Structural and functional analysis of the promoter region",
pages = "900-889",
number = "6",
volume = "166",
doi = "10.1086/432690"
}

Brkljačić, J. M., Majić, D., Miljuš-Đukić, J., Bratić, A., Konstantinović, M., Milisavljević, M.,& Maksimović, V. R.. (2005). The metallothionein-like gene from buckwheat: Structural and functional analysis of the promoter region. in International Journal of Plant Sciences
Univ Chicago Press, Chicago., 166(6), 889-900.
https://doi.org/10.1086/432690

Brkljačić JM, Majić D, Miljuš-Đukić J, Bratić A, Konstantinović M, Milisavljević M, Maksimović VR. The metallothionein-like gene from buckwheat: Structural and functional analysis of the promoter region. in International Journal of Plant Sciences. 2005;166(6):889-900.
doi:10.1086/432690 .

Brkljačić, Jelena M., Majić, Dragana, Miljuš-Đukić, Jovanka, Bratić, A, Konstantinović, MM, Milisavljević, Mira, Maksimović, Vesna R., "The metallothionein-like gene from buckwheat: Structural and functional analysis of the promoter region" in International Journal of Plant Sciences, 166, no. 6 (2005):889-900,
https://doi.org/10.1086/432690 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Konstantinović, Miroslav M.
AU  - Brkljačić, Jelena M.
AU  - Maksimović, Vesna R.
PY  - 2004
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/207
AB  - 5’-regulatorni region gena za rezervni globulin heljde (FeLEG1), dužine 955 bp, izolovan je po umnožavanju fragmenta genomske DNK heljde primenom metode 5’-RACE. Kompletan fragment je sekvenciran i sekvenca je komjuterski analizirana u smislu definisanja potencijalnih cys-regulatornih elemenata uključenih u tkivno specifičnu i u razviću determinisanu gensku ekspresiju. Izolovani promotor je interesantan ne samo za fundamentalna istraživanja već i kao potencijalno upotrebljiv za biotehnološku primenu.
AB  - Using the modified 5’-RACE approach, a fragment containing the 955 bp long 5’- regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory elements possibly involved in tissue specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research, but also as a useful tool for biotechnological application.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Kloniranje i kompjuterska analiza promotorskog regiona gena za rezervni protein heljde leguminskog tipa
T1  - Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench
EP  - 7
IS  - 1-2
SP  - 1
VL  - 56
UR  - https://hdl.handle.net/21.15107/rcub_imagine_207
ER  - 

@article{
author = "Milisavljević, Mira and Konstantinović, Miroslav M. and Brkljačić, Jelena M. and Maksimović, Vesna R.",
year = "2004",
abstract = "5’-regulatorni region gena za rezervni globulin heljde (FeLEG1), dužine 955 bp, izolovan je po umnožavanju fragmenta genomske DNK heljde primenom metode 5’-RACE. Kompletan fragment je sekvenciran i sekvenca je komjuterski analizirana u smislu definisanja potencijalnih cys-regulatornih elemenata uključenih u tkivno specifičnu i u razviću determinisanu gensku ekspresiju. Izolovani promotor je interesantan ne samo za fundamentalna istraživanja već i kao potencijalno upotrebljiv za biotehnološku primenu., Using the modified 5’-RACE approach, a fragment containing the 955 bp long 5’- regulatory region of the buckwheat storage globulin gene (FeLEG1) has been amplified from the genomic DNA of buckwheat. The entire fragment was sequenced and the sequence analyzed by computer prediction of cis-regulatory elements possibly involved in tissue specific and developmentally controlled seed storage protein gene expression. The promoter obtained might be interesting not only for fundamental research, but also as a useful tool for biotechnological application.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Kloniranje i kompjuterska analiza promotorskog regiona gena za rezervni protein heljde leguminskog tipa, Cloning and computer analysis of the promoter region of the legumin-like storage protein gene from buckwheat, Fagopyrum esculentum Moench",
pages = "7-1",
number = "1-2",
volume = "56",
url = "https://hdl.handle.net/21.15107/rcub_imagine_207"
}

Milisavljević, M., Konstantinović, M. M., Brkljačić, J. M.,& Maksimović, V. R.. (2004). Kloniranje i kompjuterska analiza promotorskog regiona gena za rezervni protein heljde leguminskog tipa. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 56(1-2), 1-7.
https://hdl.handle.net/21.15107/rcub_imagine_207

Milisavljević M, Konstantinović MM, Brkljačić JM, Maksimović VR. Kloniranje i kompjuterska analiza promotorskog regiona gena za rezervni protein heljde leguminskog tipa. in Archives of Biological Sciences. 2004;56(1-2):1-7.
https://hdl.handle.net/21.15107/rcub_imagine_207 .

Milisavljević, Mira, Konstantinović, Miroslav M., Brkljačić, Jelena M., Maksimović, Vesna R., "Kloniranje i kompjuterska analiza promotorskog regiona gena za rezervni protein heljde leguminskog tipa" in Archives of Biological Sciences, 56, no. 1-2 (2004):1-7,
https://hdl.handle.net/21.15107/rcub_imagine_207 .

TY  - JOUR
AU  - Brkljačić, Jelena M.
AU  - Samardžić, Jelena
AU  - Timotijević, Gordana
AU  - Maksimović, Vesna R.
PY  - 2004
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/194
AB  - The buckwheat metallothionein-like (MT3) gene expression was studied throughout seed and leaf development, as well as under the influence of different external stimuli. MT3 mRNAs were detected from the early stage of seed development to the end of maturation, reaching the highest level during the mid-maturation stage. High MT3 mRNA level was noticed for both green and senescent leaves. The influence of raising Cu ion concentrations on MT3 gene expression was studied only in leaves, while the effect of Zn ions was analyzed through seed development as well. It was found that Cu and Zn ions had stimulatory effects on expression in leaves. MT3 expression was significantly enhanced in the early stage of seed development in response to Zn ions, while after this stage, influence of Zn ions was not detected. After H2O2/NaCl treatment, MT3 mRNA level was decreased in green leaves, contrary to senescent leaves where expression levels remained unchanged. H2O2 treatment caused the increase of MT3 mRNA levels in the mid-maturation stage of seed development. NaCl had no effect on expression levels in seeds. According to obtained results, proposed functions in different plant organs regarding oxidative stress and metal homeostasis are discussed.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions
EP  - 746
IS  - 6
SP  - 741
VL  - 161
DO  - 10.1078/0176-1617-01211
ER  - 

@article{
author = "Brkljačić, Jelena M. and Samardžić, Jelena and Timotijević, Gordana and Maksimović, Vesna R.",
year = "2004",
abstract = "The buckwheat metallothionein-like (MT3) gene expression was studied throughout seed and leaf development, as well as under the influence of different external stimuli. MT3 mRNAs were detected from the early stage of seed development to the end of maturation, reaching the highest level during the mid-maturation stage. High MT3 mRNA level was noticed for both green and senescent leaves. The influence of raising Cu ion concentrations on MT3 gene expression was studied only in leaves, while the effect of Zn ions was analyzed through seed development as well. It was found that Cu and Zn ions had stimulatory effects on expression in leaves. MT3 expression was significantly enhanced in the early stage of seed development in response to Zn ions, while after this stage, influence of Zn ions was not detected. After H2O2/NaCl treatment, MT3 mRNA level was decreased in green leaves, contrary to senescent leaves where expression levels remained unchanged. H2O2 treatment caused the increase of MT3 mRNA levels in the mid-maturation stage of seed development. NaCl had no effect on expression levels in seeds. According to obtained results, proposed functions in different plant organs regarding oxidative stress and metal homeostasis are discussed.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions",
pages = "746-741",
number = "6",
volume = "161",
doi = "10.1078/0176-1617-01211"
}

Brkljačić, J. M., Samardžić, J., Timotijević, G.,& Maksimović, V. R.. (2004). Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 161(6), 741-746.
https://doi.org/10.1078/0176-1617-01211

Brkljačić JM, Samardžić J, Timotijević G, Maksimović VR. Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions. in Journal of Plant Physiology. 2004;161(6):741-746.
doi:10.1078/0176-1617-01211 .

Brkljačić, Jelena M., Samardžić, Jelena, Timotijević, Gordana, Maksimović, Vesna R., "Expression analysis of buckwheat (Fagopyrum esculentum Moench) metallothionein-like gene (MT3) under different stress and physiological conditions" in Journal of Plant Physiology, 161, no. 6 (2004):741-746,
https://doi.org/10.1078/0176-1617-01211 . .

TY  - JOUR
AU  - Milisavljević, Mira
AU  - Timotijević, Gordana
AU  - Radović, SR
AU  - Brkljačić, Jelena M.
AU  - Konstantinović, MM
AU  - Maksimović, Vesna R.
PY  - 2004
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/192
AB  - An 8S storage globulin from buckwheat seed, which resembles the structure and features common to the vicilin-like family of seed storage proteins, was analyzed for this paper. It was found that expression of the 8S globulin gene precedes that of the 13S globulin (the main buckwheat storage protein) and starts from an early stage of buckwheat seed development (9-11 days after flowering), continuing to accumulate throughout seed development to contribute similar to7% of total seed proteins. This protein fraction might be more interesting for biotechnological application than the 13S buckwheat legumin consisting of 23-25 kDa subunits reported to be the major buckwheat allergen. A partial cDNA was also isolated, showing high homology with cDNAs coding for vicilin-like storage proteins from various plant species, and its expression profile throughout seed development as well as in different buckwheat tissues was analyzed.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds
EP  - 5262
IS  - 16
SP  - 5258
VL  - 52
DO  - 10.1021/jf049519v
ER  - 

@article{
author = "Milisavljević, Mira and Timotijević, Gordana and Radović, SR and Brkljačić, Jelena M. and Konstantinović, MM and Maksimović, Vesna R.",
year = "2004",
abstract = "An 8S storage globulin from buckwheat seed, which resembles the structure and features common to the vicilin-like family of seed storage proteins, was analyzed for this paper. It was found that expression of the 8S globulin gene precedes that of the 13S globulin (the main buckwheat storage protein) and starts from an early stage of buckwheat seed development (9-11 days after flowering), continuing to accumulate throughout seed development to contribute similar to7% of total seed proteins. This protein fraction might be more interesting for biotechnological application than the 13S buckwheat legumin consisting of 23-25 kDa subunits reported to be the major buckwheat allergen. A partial cDNA was also isolated, showing high homology with cDNAs coding for vicilin-like storage proteins from various plant species, and its expression profile throughout seed development as well as in different buckwheat tissues was analyzed.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds",
pages = "5262-5258",
number = "16",
volume = "52",
doi = "10.1021/jf049519v"
}

Milisavljević, M., Timotijević, G., Radović, S., Brkljačić, J. M., Konstantinović, M.,& Maksimović, V. R.. (2004). Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 52(16), 5258-5262.
https://doi.org/10.1021/jf049519v

Milisavljević M, Timotijević G, Radović S, Brkljačić JM, Konstantinović M, Maksimović VR. Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds. in Journal of Agricultural and Food Chemistry. 2004;52(16):5258-5262.
doi:10.1021/jf049519v .

Milisavljević, Mira, Timotijević, Gordana, Radović, SR, Brkljačić, Jelena M., Konstantinović, MM, Maksimović, Vesna R., "Vicilin-like storage globulin from buckwheat (Fagopyrum esculentum Moench) seeds" in Journal of Agricultural and Food Chemistry, 52, no. 16 (2004):5258-5262,
https://doi.org/10.1021/jf049519v . .