TY  - JOUR
AU  - Pantelić, Brana
AU  - Siaperas, Romanos
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14445
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2337
AB  - Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.
PB  - Wiley
T2  - Microbial Biotechnology
T2  - Microbial Biotechnology
T1  - Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production
IS  - 3
SP  - e14445
VL  - 17
DO  - 10.1111/1751-7915.14445
ER  - 

@article{
author = "Pantelić, Brana and Siaperas, Romanos and Budin, Clémence and de Boer, Tjalf and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.",
publisher = "Wiley",
journal = "Microbial Biotechnology, Microbial Biotechnology",
title = "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production",
number = "3",
pages = "e14445",
volume = "17",
doi = "10.1111/1751-7915.14445"
}

Pantelić, B., Siaperas, R., Budin, C., de Boer, T., Topakas, E.,& Nikodinović-Runić, J.. (2024). Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology
Wiley., 17(3), e14445.
https://doi.org/10.1111/1751-7915.14445

Pantelić B, Siaperas R, Budin C, de Boer T, Topakas E, Nikodinović-Runić J. Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology. 2024;17(3):e14445.
doi:10.1111/1751-7915.14445 .

Pantelić, Brana, Siaperas, Romanos, Budin, Clémence, de Boer, Tjalf, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production" in Microbial Biotechnology, 17, no. 3 (2024):e14445,
https://doi.org/10.1111/1751-7915.14445 . .

TY  - JOUR
AU  - Araujo, Jeovan A.
AU  - Taxeidis, George
AU  - Pereira, Everton H.
AU  - Azeem, Muhammad
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Ponjavić, Marijana
AU  - Chen, Yuanyuan
AU  - Mojicević, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
AU  - Brennan Fournet, Margaret
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0959652624004724
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2315
AB  - Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.
PB  - Elsevier
T2  - Journal of Cleaner Production
T1  - Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose
SP  - 141025
DO  - 10.1016/j.jclepro.2024.141025
ER  - 

@article{
author = "Araujo, Jeovan A. and Taxeidis, George and Pereira, Everton H. and Azeem, Muhammad and Pantelić, Brana and Jeremić, Sanja and Ponjavić, Marijana and Chen, Yuanyuan and Mojicević, Marija and Nikodinović-Runić, Jasmina and Topakas, Evangelos and Brennan Fournet, Margaret",
year = "2024",
abstract = "Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.",
publisher = "Elsevier",
journal = "Journal of Cleaner Production",
title = "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose",
pages = "141025",
doi = "10.1016/j.jclepro.2024.141025"
}

Araujo, J. A., Taxeidis, G., Pereira, E. H., Azeem, M., Pantelić, B., Jeremić, S., Ponjavić, M., Chen, Y., Mojicević, M., Nikodinović-Runić, J., Topakas, E.,& Brennan Fournet, M.. (2024). Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production
Elsevier., 141025.
https://doi.org/10.1016/j.jclepro.2024.141025

Araujo JA, Taxeidis G, Pereira EH, Azeem M, Pantelić B, Jeremić S, Ponjavić M, Chen Y, Mojicević M, Nikodinović-Runić J, Topakas E, Brennan Fournet M. Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production. 2024;:141025.
doi:10.1016/j.jclepro.2024.141025 .

Araujo, Jeovan A., Taxeidis, George, Pereira, Everton H., Azeem, Muhammad, Pantelić, Brana, Jeremić, Sanja, Ponjavić, Marijana, Chen, Yuanyuan, Mojicević, Marija, Nikodinović-Runić, Jasmina, Topakas, Evangelos, Brennan Fournet, Margaret, "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose" in Journal of Cleaner Production (2024):141025,
https://doi.org/10.1016/j.jclepro.2024.141025 . .

TY  - CONF
AU  - Janković, Vukašin
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Kalogirou, Charalampia
AU  - Ilić-Tomić, Tatjana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2371
AB  - The increasing production and utilization of
synthetic polymers in the textile industry over
the past five decades has raised concerns about
the environmental impact of the industry. The
recalcitrant nature of synthetic fibers hampers
the biodegradation of these textiles in the environment
and leads to the accumulation of textile
waste. Effective solutions for recycling and proper
disposal of textile waste are lacking, however,
the use of microorganisms and enzymes has
emerged as a promising approach. The genus
Streptomyces has been well studied as a producer
of different hydrolytic enzymes, several of which
have found use in industrial settings as well. As
an integral part of the soil microbiome, Streptomyces
species have been shown to interact with
different textile materials in soil and may play a
role in the degradation of these materials. This
study aimed to examine the interaction of Streptomyces
sp. R1, isolated from the rhizosphere of
Cotinus coggygria, with polyamide/polyurethane
textile, and identify potential enzymes involved in the biodegradation of synthetic textiles. The
degradation of the textile was tested in liquid
cultures (minimal salt medium) and model compost,
bio-augmented with Streptomyces sp. R1
for 4 months. After the incubation, morphological,
and changes in the functional groups of the
textiles were analysed using scanning electron
microscopy (SEM) and Fourier transform infrared
spectroscopy (FTIR). The surface of the textile
showed noticeable cracks and fissures after
4 months of burial in the bioaugmented model
compost, alongside changes in the functional
groups of the polyamide/polyurethane textile,
which indicates biodegradation of the synthetic
fibers. Searching the genome of Streptomyces sp.
R1, several enzymes involved in the degradation
of synthetic polymers were identified, including
an esterase homologous to highly efficient plastic
degrading depolymerases. Overall, the results
presented here indicate Streptomyces sp. R1 has
the potential for synthetic textile degradation
and bioremediation.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2371
ER  - 

@conference{
author = "Janković, Vukašin and Pantelić, Brana and Jeremić, Sanja and Radetić, Maja and Marković, Darka and Kalogirou, Charalampia and Ilić-Tomić, Tatjana",
year = "2024",
abstract = "The increasing production and utilization of
synthetic polymers in the textile industry over
the past five decades has raised concerns about
the environmental impact of the industry. The
recalcitrant nature of synthetic fibers hampers
the biodegradation of these textiles in the environment
and leads to the accumulation of textile
waste. Effective solutions for recycling and proper
disposal of textile waste are lacking, however,
the use of microorganisms and enzymes has
emerged as a promising approach. The genus
Streptomyces has been well studied as a producer
of different hydrolytic enzymes, several of which
have found use in industrial settings as well. As
an integral part of the soil microbiome, Streptomyces
species have been shown to interact with
different textile materials in soil and may play a
role in the degradation of these materials. This
study aimed to examine the interaction of Streptomyces
sp. R1, isolated from the rhizosphere of
Cotinus coggygria, with polyamide/polyurethane
textile, and identify potential enzymes involved in the biodegradation of synthetic textiles. The
degradation of the textile was tested in liquid
cultures (minimal salt medium) and model compost,
bio-augmented with Streptomyces sp. R1
for 4 months. After the incubation, morphological,
and changes in the functional groups of the
textiles were analysed using scanning electron
microscopy (SEM) and Fourier transform infrared
spectroscopy (FTIR). The surface of the textile
showed noticeable cracks and fissures after
4 months of burial in the bioaugmented model
compost, alongside changes in the functional
groups of the polyamide/polyurethane textile,
which indicates biodegradation of the synthetic
fibers. Searching the genome of Streptomyces sp.
R1, several enzymes involved in the degradation
of synthetic polymers were identified, including
an esterase homologous to highly efficient plastic
degrading depolymerases. Overall, the results
presented here indicate Streptomyces sp. R1 has
the potential for synthetic textile degradation
and bioremediation.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2371"
}

Janković, V., Pantelić, B., Jeremić, S., Radetić, M., Marković, D., Kalogirou, C.,& Ilić-Tomić, T.. (2024). DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2371

Janković V, Pantelić B, Jeremić S, Radetić M, Marković D, Kalogirou C, Ilić-Tomić T. DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2371 .

Janković, Vukašin, Pantelić, Brana, Jeremić, Sanja, Radetić, Maja, Marković, Darka, Kalogirou, Charalampia, Ilić-Tomić, Tatjana, "DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2371 .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Araujo, Jeovan
AU  - Jeremić, Sanja
AU  - Azeem, Muhammad
AU  - Attallah, Olivia
AU  - Slaperas, Romanos
AU  - Mojicević, Marija
AU  - Chen, Yuanyuan
AU  - Fournet, Margaret Brennan
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2352186423003127
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1983
AB  - Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.
PB  - Elsevier
T2  - Environmental Technology & Innovation
T1  - A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate
SP  - 103316
DO  - 10.1016/j.eti.2023.103316
ER  - 

@article{
author = "Pantelić, Brana and Araujo, Jeovan and Jeremić, Sanja and Azeem, Muhammad and Attallah, Olivia and Slaperas, Romanos and Mojicević, Marija and Chen, Yuanyuan and Fournet, Margaret Brennan and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.",
publisher = "Elsevier",
journal = "Environmental Technology & Innovation",
title = "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate",
pages = "103316",
doi = "10.1016/j.eti.2023.103316"
}

Pantelić, B., Araujo, J., Jeremić, S., Azeem, M., Attallah, O., Slaperas, R., Mojicević, M., Chen, Y., Fournet, M. B., Topakas, E.,& Nikodinović-Runić, J.. (2023). A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation
Elsevier., 103316.
https://doi.org/10.1016/j.eti.2023.103316

Pantelić B, Araujo J, Jeremić S, Azeem M, Attallah O, Slaperas R, Mojicević M, Chen Y, Fournet MB, Topakas E, Nikodinović-Runić J. A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation. 2023;:103316.
doi:10.1016/j.eti.2023.103316 .

Pantelić, Brana, Araujo, Jeovan, Jeremić, Sanja, Azeem, Muhammad, Attallah, Olivia, Slaperas, Romanos, Mojicević, Marija, Chen, Yuanyuan, Fournet, Margaret Brennan, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate" in Environmental Technology & Innovation (2023):103316,
https://doi.org/10.1016/j.eti.2023.103316 . .

TY  - CONF
AU  - Ćirić, Milica
AU  - Pantelić, Brana
AU  - Šaraba, Vladimir
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2044
AB  - Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous
synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the
soil and water environments. Highly efficient PU-degrading microorganisms are rare in
nature and are of fundamental importance for achieving circular plastic economy. Bacterial
isolates from groundwater, originating from magmatogenic massif and Tertiary basin
within metamorphic area, as well as soil isolates collected from various pristine (PS) and
contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD
(IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA
gene sequences from IMP-degrading isolates was performed using the neighbor-joining
method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and
18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading
isolates from each type of environment, belonging to 8 genera (Pseudomonas,
Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas)
for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter,
Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No
clear grouping of groundwater and soil isolates was observed, indicating that isolates are
too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For
groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus,
2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering
was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping
within isolates from CS and PS. In the future, wider range of environmental niches should be
included in screening efforts for development of biocatalytic processes for management of
plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and
represent largely unexplored reservoir of biotechnologically relevant enzymatic activities,
should also be more represented in such screenings.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Groundwater and soil as a reservoir for polyurethane-degrading bacteria
EP  - 99
SP  - 99
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2044
ER  - 

@conference{
author = "Ćirić, Milica and Pantelić, Brana and Šaraba, Vladimir and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous
synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the
soil and water environments. Highly efficient PU-degrading microorganisms are rare in
nature and are of fundamental importance for achieving circular plastic economy. Bacterial
isolates from groundwater, originating from magmatogenic massif and Tertiary basin
within metamorphic area, as well as soil isolates collected from various pristine (PS) and
contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD
(IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA
gene sequences from IMP-degrading isolates was performed using the neighbor-joining
method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and
18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading
isolates from each type of environment, belonging to 8 genera (Pseudomonas,
Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas)
for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter,
Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No
clear grouping of groundwater and soil isolates was observed, indicating that isolates are
too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For
groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus,
2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering
was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping
within isolates from CS and PS. In the future, wider range of environmental niches should be
included in screening efforts for development of biocatalytic processes for management of
plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and
represent largely unexplored reservoir of biotechnologically relevant enzymatic activities,
should also be more represented in such screenings.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Groundwater and soil as a reservoir for polyurethane-degrading bacteria",
pages = "99-99",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2044"
}

Ćirić, M., Pantelić, B., Šaraba, V.,& Nikodinović-Runić, J.. (2023). Groundwater and soil as a reservoir for polyurethane-degrading bacteria. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2044

Ćirić M, Pantelić B, Šaraba V, Nikodinović-Runić J. Groundwater and soil as a reservoir for polyurethane-degrading bacteria. in 4th Belgrade Bioinformatics Conference. 2023;4:99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2044 .

Ćirić, Milica, Pantelić, Brana, Šaraba, Vladimir, Nikodinović-Runić, Jasmina, "Groundwater and soil as a reservoir for polyurethane-degrading bacteria" in 4th Belgrade Bioinformatics Conference, 4 (2023):99-99,
https://hdl.handle.net/21.15107/rcub_imagine_2044 .

TY  - CONF
AU  - Stevanović, Milena
AU  - Pantelić, Brana
AU  - Janković, Vukašin
AU  - Nikodinović-Runić, Jasmina
AU  - Vojnović, Sandra
PY  - 2023
UR  - https://www.fems2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2022
AB  - Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.
C3  - FEMS2023 Congress of European Microbiologists
T1  - Expression of PET-hydrolyzing enzymes in Streptomyces spp.
EP  - 836
SP  - 836
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2022
ER  - 

@conference{
author = "Stevanović, Milena and Pantelić, Brana and Janković, Vukašin and Nikodinović-Runić, Jasmina and Vojnović, Sandra",
year = "2023",
abstract = "Plastic waste has become a serious global challenge that calls for sustainable solutions and requires
rapid actions. Biocatalysis could present an adequate answer to this problem by providing different
enzymes capable of degrading plastic polymers. Streptomyces strains as predominant soil
inhabitants have also adapted to the presence of variety of plastic waste in natural environments,
so they have been examined for the plastic degrading capabilities.
The aim of this work was to improve the biocatalytic properties of Streptomyces strains for their
use in biodegradation of plastic polymers and develop a system for heterologous expression of
polyethylene therephtalate (PET) degrading enzymes in Streptomyces spp.
Well studied Streptomyces lividans TK24 and S. albus NRRL B-1335, as well as two newly
isolated Streptomycetes were used for expression of benchmark PETases and cutinases. Enzymes
were cloned into pGM1202 Escherichia coli–Streptomyces shuttle vector and subsequently
introduced into Streptomyces hosts either by polyethylene glycol-mediated protoplasts
transformation or by electroporation. Cell-free extracts and supernatants of transformed cells were
tested on different plastics using bis(2-hydroxyethyl) terephthalate (BHET), polycaprolactone
(PCL) and Impranil as substrates in plate assays.
Expression of leaf-branch compost cutinase in S. albus and S. lividans resulted in an 8.5- and 2.5-
times increase in esterase activities, respectively. Introduction of the enzyme into newly isolated
strains that already showed some plastic degrading activity resulted in synergistic activity of the
recombinant strains.",
journal = "FEMS2023 Congress of European Microbiologists",
title = "Expression of PET-hydrolyzing enzymes in Streptomyces spp.",
pages = "836-836",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2022"
}

Stevanović, M., Pantelić, B., Janković, V., Nikodinović-Runić, J.,& Vojnović, S.. (2023). Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists, 10, 836-836.
https://hdl.handle.net/21.15107/rcub_imagine_2022

Stevanović M, Pantelić B, Janković V, Nikodinović-Runić J, Vojnović S. Expression of PET-hydrolyzing enzymes in Streptomyces spp.. in FEMS2023 Congress of European Microbiologists. 2023;10:836-836.
https://hdl.handle.net/21.15107/rcub_imagine_2022 .

Stevanović, Milena, Pantelić, Brana, Janković, Vukašin, Nikodinović-Runić, Jasmina, Vojnović, Sandra, "Expression of PET-hydrolyzing enzymes in Streptomyces spp." in FEMS2023 Congress of European Microbiologists, 10 (2023):836-836,
https://hdl.handle.net/21.15107/rcub_imagine_2022 .

TY  - DATA
AU  - Pantelić, Brana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Lončarević, Branka
AU  - Beskoski, Vladimir
AU  - Maslak, Veselin
AU  - Guzik, Maciej
AU  - Makryniotis, Konstantinos
AU  - Taxeidis, George
AU  - Siaperas, Romanos
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4344/13/2/278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1792
T2  - Catalysts
T1  - Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278
IS  - 2
SP  - 278
VL  - 13
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1792
ER  - 

@misc{
author = "Pantelić, Brana and Škaro Bogojević, Sanja and Milivojević, Dušan and Ilić-Tomić, Tatjana and Lončarević, Branka and Beskoski, Vladimir and Maslak, Veselin and Guzik, Maciej and Makryniotis, Konstantinos and Taxeidis, George and Siaperas, Romanos and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
journal = "Catalysts",
title = "Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278",
number = "2",
pages = "278",
volume = "13",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1792"
}

Pantelić, B., Škaro Bogojević, S., Milivojević, D., Ilić-Tomić, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E.,& Nikodinović-Runić, J.. (2023). Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278. in Catalysts, 13(2), 278.
https://hdl.handle.net/21.15107/rcub_imagine_1792

Pantelić B, Škaro Bogojević S, Milivojević D, Ilić-Tomić T, Lončarević B, Beskoski V, Maslak V, Guzik M, Makryniotis K, Taxeidis G, Siaperas R, Topakas E, Nikodinović-Runić J. Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278. in Catalysts. 2023;13(2):278.
https://hdl.handle.net/21.15107/rcub_imagine_1792 .

Pantelić, Brana, Škaro Bogojević, Sanja, Milivojević, Dušan, Ilić-Tomić, Tatjana, Lončarević, Branka, Beskoski, Vladimir, Maslak, Veselin, Guzik, Maciej, Makryniotis, Konstantinos, Taxeidis, George, Siaperas, Romanos, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Supporting information: Pantelic, B., Skaro Bogojevic, S., Milivojevic, D., Ilic-Tomic, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E., & Nikodinovic-Runic, J. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. Catalysts, 13(2), Art. 2. https://doi.org/10.3390/catal13020278" in Catalysts, 13, no. 2 (2023):278,
https://hdl.handle.net/21.15107/rcub_imagine_1792 .

TY  - CONF
AU  - Milovanović, Jelena
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Ponjavić,  Marijana
AU  - Sourkouni, Georgia
AU  - Kalogirou, Charalampia
AU  - Argirusis, Christos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://esabweb.org/E_CONGRESS/Poster+Programme/Day/Online/All+day/Enhanced+enzymatic+depolymerization+of+polylactic+acid+%28PLA%29+through+plasma+pretreatment+and+subsequent+conversion+to+biopolymer.html
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2232
AB  - Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
PB  - European Society of Applied Biocatalysis
C3  - ESAB E-Congress
T1  - Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2232
ER  - 

@conference{
author = "Milovanović, Jelena and Nenadović, Marija and Pantelić, Brana and Ponjavić,  Marijana and Sourkouni, Georgia and Kalogirou, Charalampia and Argirusis, Christos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polylactic acid (PLA) serves as a bio-based alternative to fossil-based single-use plastics, biodegrading at high temperatures (58°C) and humidity during industrial composting. Despite enzymes' ability to catalyze reactions at near-ambient temperatures, polymer rigidity can impede efficient depolymerization. To address these challenges, we conducted a study of enzymatic PLA degradation at 42°C combined with green plasma pretreatment to help disrupt the crystalline regions within the polymer. Here we report the effect of length of plasma pretreatment on the rate of PLA degradation by enzyme mix containing commercial enzymes with reported PLA degrading activity. Results indicate that a 5-minute plasma pretreatment significantly enhances enzymatic degradation, with a 16% weight loss achieved in 4 weeks—a two-fold increase compared to untreated PLA. Furthermore, we report the valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
publisher = "European Society of Applied Biocatalysis",
journal = "ESAB E-Congress",
title = "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2232"
}

Milovanović, J., Nenadović, M., Pantelić, B., Ponjavić, M., Sourkouni, G., Kalogirou, C., Argirusis, C.,& Nikodinović-Runić, J.. (2023). Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress
European Society of Applied Biocatalysis..
https://hdl.handle.net/21.15107/rcub_imagine_2232

Milovanović J, Nenadović M, Pantelić B, Ponjavić M, Sourkouni G, Kalogirou C, Argirusis C, Nikodinović-Runić J. Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer. in ESAB E-Congress. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

Milovanović, Jelena, Nenadović, Marija, Pantelić, Brana, Ponjavić,  Marijana, Sourkouni, Georgia, Kalogirou, Charalampia, Argirusis, Christos, Nikodinović-Runić, Jasmina, "Enhanced enzymatic depolymerization of polylactic acid (PLA) through plasma pretreatment and subsequent conversion to biopolymer" in ESAB E-Congress (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2232 .

TY  - CONF
AU  - Nenadović, Marija
AU  - Pantelić, Brana
AU  - Lazić, Jelena
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Milovanović, Jelena
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2237
AB  - PHAs are naturally made microbial polyesters that
have been commercialized as biodegradable
plastics. However, it has been shown that these
materials are not so easily biodegraded in natural
environments [1]. PHA depolymerases are key PHA
degrading enzymes and their identification and
characterization is of great interest and importance.
Currently, screening is done on polymeric
substrates using techniques such as clear zone
assays on agar or weight loss measurements.
Results obtained using these different methods
cannot be directly compared, since they depend
highly on the polymer used, PHA granules
preparation and assay conditions [2].
In order to design a more specific test for the
determination of PHA depolymerase activity, we
synthesized 3-hyoxyalkanoate monomers (3-HA
monomer) and 3-hyoxyalkanoic acid dimers (3-HA
dimer) and their respective p-nitrophenyl esters,
allowing for spectrophotometric determination of
their activity [3]. Compounds were characterized
using N and FTIR. Para-nitrophenyl labeled
substrates were then used in the enzymatic activity
assay with the benchmark polyhyoxyoctanoate
(PHO) depolymerase from Pseudomonas
fluorescens GK13 expressed in Escherichia coli
CodonPlus-RIPL hosts. This activity was compared
to recombinantly expressed leaf-branch compost
cutinase (LCC cutinase) and
polyethyleneterephtalate (PET) hyolyzing esterase
from Ideonella sakaiensis (IsPETase). Our initial
results revealed increased specificity of PHO
depolymerase towards newly synthetized
substrates, suggesting their suitability for specific
screens and isolation of new mcl-PHA
depolymerases, as well as in high throughput
screening assays designed for guiding their
directed evolution.
C3  - 10th Conference of Mikrobiokosmos
T1  - Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2237
ER  - 

@conference{
author = "Nenadović, Marija and Pantelić, Brana and Lazić, Jelena and Maslak, Veselin and Nikodinović-Runić, Jasmina and Milovanović, Jelena",
year = "2023",
abstract = "PHAs are naturally made microbial polyesters that
have been commercialized as biodegradable
plastics. However, it has been shown that these
materials are not so easily biodegraded in natural
environments [1]. PHA depolymerases are key PHA
degrading enzymes and their identification and
characterization is of great interest and importance.
Currently, screening is done on polymeric
substrates using techniques such as clear zone
assays on agar or weight loss measurements.
Results obtained using these different methods
cannot be directly compared, since they depend
highly on the polymer used, PHA granules
preparation and assay conditions [2].
In order to design a more specific test for the
determination of PHA depolymerase activity, we
synthesized 3-hyoxyalkanoate monomers (3-HA
monomer) and 3-hyoxyalkanoic acid dimers (3-HA
dimer) and their respective p-nitrophenyl esters,
allowing for spectrophotometric determination of
their activity [3]. Compounds were characterized
using N and FTIR. Para-nitrophenyl labeled
substrates were then used in the enzymatic activity
assay with the benchmark polyhyoxyoctanoate
(PHO) depolymerase from Pseudomonas
fluorescens GK13 expressed in Escherichia coli
CodonPlus-RIPL hosts. This activity was compared
to recombinantly expressed leaf-branch compost
cutinase (LCC cutinase) and
polyethyleneterephtalate (PET) hyolyzing esterase
from Ideonella sakaiensis (IsPETase). Our initial
results revealed increased specificity of PHO
depolymerase towards newly synthetized
substrates, suggesting their suitability for specific
screens and isolation of new mcl-PHA
depolymerases, as well as in high throughput
screening assays designed for guiding their
directed evolution.",
journal = "10th Conference of Mikrobiokosmos",
title = "Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2237"
}

Nenadović, M., Pantelić, B., Lazić, J., Maslak, V., Nikodinović-Runić, J.,& Milovanović, J.. (2023). Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases. in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2237

Nenadović M, Pantelić B, Lazić J, Maslak V, Nikodinović-Runić J, Milovanović J. Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases. in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2237 .

Nenadović, Marija, Pantelić, Brana, Lazić, Jelena, Maslak, Veselin, Nikodinović-Runić, Jasmina, Milovanović, Jelena, "Medium chain length polyhyoxyalkanoates (mcl-PHA) model compounds for the discovery of novel PHA depolymerases" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2237 .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Babu P., Ramesh
AU  - Rajasekaran, Divya
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.accelevents.com/e/circular-bioeconomy-2023#about
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1924
AB  - Large amounts of polymers are discarded worldwide each year, leading to a significant
polymer waste in natural environment. The upcycling has been found as an efficient way to
transform polymer waste into high-value biomaterials meeting the conditions required for
circularity by being indefinitely recyclable, without reduction in value or usability.
The presented study refers to the upcycling of commercial biopolymers into bacterial
nanocellulose. Polymer blends, consisted of biodegradable polymers, such as poly(lactic acid),
PLA, poly(butylene succinate), PBS, and poly(ε-caprolactone), PCL. Polymers were hydrolyzed
and the obtained hydrolysates were investigated as potential carbon source for
K. medellinensis ID13488 growth and nanocellulose production. Degradation products were
analyzed using HPLC analysis. Different growth media, including tap water, HS medium,
absence / presence of glucose, were tested and bacterial nanocellulose growth was
confirmed under the most of the tested conditions. Once the BNC growth was set up, the BNC
production was scaled up and the obtained material was investigated in terms of structure
confirmation (FTIR analysis), thermal properties (DSC/TG analysis), morphology (optical
microscopy, AFM analysis) and crystallinity (XRD analysis). Finally, the full life cycle of mixed
biopolymers: from biodegradation to revalorization of end products into bacterial
nanocellulose appeared as perfect model approach to plastic circularity.
C3  - Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming
T1  - Revalorization of biodegradable polymers to valuable bacterial nanocellulose
SP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1924
ER  - 

@conference{
author = "Ponjavić, Marijana and Babu P., Ramesh and Rajasekaran, Divya and Pantelić, Brana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Large amounts of polymers are discarded worldwide each year, leading to a significant
polymer waste in natural environment. The upcycling has been found as an efficient way to
transform polymer waste into high-value biomaterials meeting the conditions required for
circularity by being indefinitely recyclable, without reduction in value or usability.
The presented study refers to the upcycling of commercial biopolymers into bacterial
nanocellulose. Polymer blends, consisted of biodegradable polymers, such as poly(lactic acid),
PLA, poly(butylene succinate), PBS, and poly(ε-caprolactone), PCL. Polymers were hydrolyzed
and the obtained hydrolysates were investigated as potential carbon source for
K. medellinensis ID13488 growth and nanocellulose production. Degradation products were
analyzed using HPLC analysis. Different growth media, including tap water, HS medium,
absence / presence of glucose, were tested and bacterial nanocellulose growth was
confirmed under the most of the tested conditions. Once the BNC growth was set up, the BNC
production was scaled up and the obtained material was investigated in terms of structure
confirmation (FTIR analysis), thermal properties (DSC/TG analysis), morphology (optical
microscopy, AFM analysis) and crystallinity (XRD analysis). Finally, the full life cycle of mixed
biopolymers: from biodegradation to revalorization of end products into bacterial
nanocellulose appeared as perfect model approach to plastic circularity.",
journal = "Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming",
title = "Revalorization of biodegradable polymers to valuable bacterial nanocellulose",
pages = "55",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1924"
}

Ponjavić, M., Babu P., R., Rajasekaran, D., Pantelić, B.,& Nikodinović-Runić, J.. (2023). Revalorization of biodegradable polymers to valuable bacterial nanocellulose. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming, 55.
https://hdl.handle.net/21.15107/rcub_imagine_1924

Ponjavić M, Babu P. R, Rajasekaran D, Pantelić B, Nikodinović-Runić J. Revalorization of biodegradable polymers to valuable bacterial nanocellulose. in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming. 2023;:55.
https://hdl.handle.net/21.15107/rcub_imagine_1924 .

Ponjavić, Marijana, Babu P., Ramesh, Rajasekaran, Divya, Pantelić, Brana, Nikodinović-Runić, Jasmina, "Revalorization of biodegradable polymers to valuable bacterial nanocellulose" in Biotechnology for a circular bioeconomy: carbon capture, waste recycling and mitigation of global warming (2023):55,
https://hdl.handle.net/21.15107/rcub_imagine_1924 .

TY  - CONF
AU  - Marija, Nenadović
AU  - Pantelić, Brana
AU  - Ponjavić, Marijana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1916
AB  - Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for mechanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers
EP  - 196
SP  - 195
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1916
ER  - 

@conference{
author = "Marija, Nenadović and Pantelić, Brana and Ponjavić, Marijana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyhydroxyalkanoates (PHA) are a green substitute for conventional plastics,
owing to their biological origin, biodegradability, biocompatibility and structural
diversity. However, environmental biodegradation of PHA is achieved in a time
frame of several months to several years, depending on environmental conditions,
and properties of both PHA and PHA degrading enzymes (PhaZ) [1]. Taking into
account the high production cost of PHA, landfilling at the end of life is not likely to
be cost-effective, so enzymatic biodegradation as an alternative offers an ecofriendly
bio-cyclable route to cost-effective PHA. Our study aims to tailor PhaZ
properties to create suitable biocatalysts for the industrially relevant time frame and
operating conditions. In order to do so, we decided to randomize PhaZ sequences
and functionally screen enzyme variants for accelerated PHA degradation and
improved biocatalyst stability. Up to this day, various phaZ genes have been mutated
solely for mechanistic purposes eg. Catalytic residue identification, and elucidation
of the substrate recognition process [2,3,4,5,6,7].",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers",
pages = "196-195",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1916"
}

Marija, N., Pantelić, B., Ponjavić, M.,& Nikodinović-Runić, J.. (2023). Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 195-196.
https://hdl.handle.net/21.15107/rcub_imagine_1916

Marija N, Pantelić B, Ponjavić M, Nikodinović-Runić J. Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:195-196.
https://hdl.handle.net/21.15107/rcub_imagine_1916 .

Marija, Nenadović, Pantelić, Brana, Ponjavić, Marijana, Nikodinović-Runić, Jasmina, "Exploring PhaZ depolymerase sequence space for the bio-cyclable loop for biopolymers" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):195-196,
https://hdl.handle.net/21.15107/rcub_imagine_1916 .

TY  - JOUR
AU  - Taxeidis, George
AU  - Nikolaivits, Efstratios
AU  - Siaperas, Romanos
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0269749123004621
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1800
AB  - The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.
T2  - Environmental Pollution
T1  - Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes
SP  - 121460
VL  - 325
DO  - 10.1016/j.envpol.2023.121460
ER  - 

@article{
author = "Taxeidis, George and Nikolaivits, Efstratios and Siaperas, Romanos and Gkountela, Christina and Vouyiouka, Stamatina and Pantelić, Brana and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2023",
abstract = "The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.",
journal = "Environmental Pollution",
title = "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes",
pages = "121460",
volume = "325",
doi = "10.1016/j.envpol.2023.121460"
}

Taxeidis, G., Nikolaivits, E., Siaperas, R., Gkountela, C., Vouyiouka, S., Pantelić, B., Nikodinović-Runić, J.,& Topakas, E.. (2023). Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution, 325, 121460.
https://doi.org/10.1016/j.envpol.2023.121460

Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelić B, Nikodinović-Runić J, Topakas E. Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution. 2023;325:121460.
doi:10.1016/j.envpol.2023.121460 .

Taxeidis, George, Nikolaivits, Efstratios, Siaperas, Romanos, Gkountela, Christina, Vouyiouka, Stamatina, Pantelić, Brana, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes" in Environmental Pollution, 325 (2023):121460,
https://doi.org/10.1016/j.envpol.2023.121460 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Škaro Bogojević, Sanja
AU  - Milivojević, Dušan
AU  - Ilić-Tomić, Tatjana
AU  - Lončarević, Branka
AU  - Beskoski, Vladimir
AU  - Maslak, Veselin
AU  - Guzik, Maciej
AU  - Makryniotis, Konstantinos
AU  - Taxeidis, George
AU  - Siaperas, Romanos
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.mdpi.com/2073-4344/13/2/278
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1790
AB  - Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.
T2  - Catalysts
T2  - Catalysts
T1  - Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts
IS  - 2
SP  - 278
VL  - 13
DO  - 10.3390/catal13020278
ER  - 

@article{
author = "Pantelić, Brana and Škaro Bogojević, Sanja and Milivojević, Dušan and Ilić-Tomić, Tatjana and Lončarević, Branka and Beskoski, Vladimir and Maslak, Veselin and Guzik, Maciej and Makryniotis, Konstantinos and Taxeidis, George and Siaperas, Romanos and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Polyurethanes (PUs) are an exceedingly heterogeneous group of plastic polymers, widely used in a variety of industries from construction to medical implants. In the past decades, we have witnessed the accumulation of PU waste and its detrimental environmental impacts. PUs have been identified as one of the most toxic polymers leaching hazardous compounds derived both from the polymer itself and the additives used in production. Further environmental impact assessment, identification and characterization of substances derived from PU materials and establishing efficient degradation strategies are crucial. Thus, a selection of eight synthetic model compounds which represent partial PU hydrolysis products were synthesized and characterized both in terms of toxicity and suitability to be used as substrates for the identification of novel biocatalysts for PU biodegradation. Overall, the compounds exhibited low in vitro cytotoxicity against a healthy human fibroblast cell line and virtually no toxic effect on the nematode Caenorhabditis elegans up to 500 µg mL−1, and two of the substrates showed moderate aquatic ecotoxicity with EC50 values 53 µg mL−1 and 45 µg mL−1, respectively, on Aliivibrio fischeri. The compounds were successfully applied to study the mechanism of ester and urethane bond cleaving preference of known plastic-degrading enzymes and were used to single out a novel PU-degrading biocatalyst, Amycolatopsis mediterranei ISP5501, among 220 microbial strains. A. mediterranei ISP5501 can also degrade commercially available polyether and polyester PU materials, reducing the average molecular number of the polymer up to 13.5%. This study uncovered a biocatalyst capable of degrading different types of PUs and identified potential enzymes responsible as a key step in developing biotechnological process for PU waste treatment options.",
journal = "Catalysts, Catalysts",
title = "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts",
number = "2",
pages = "278",
volume = "13",
doi = "10.3390/catal13020278"
}

Pantelić, B., Škaro Bogojević, S., Milivojević, D., Ilić-Tomić, T., Lončarević, B., Beskoski, V., Maslak, V., Guzik, M., Makryniotis, K., Taxeidis, G., Siaperas, R., Topakas, E.,& Nikodinović-Runić, J.. (2023). Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts, 13(2), 278.
https://doi.org/10.3390/catal13020278

Pantelić B, Škaro Bogojević S, Milivojević D, Ilić-Tomić T, Lončarević B, Beskoski V, Maslak V, Guzik M, Makryniotis K, Taxeidis G, Siaperas R, Topakas E, Nikodinović-Runić J. Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts. in Catalysts. 2023;13(2):278.
doi:10.3390/catal13020278 .

Pantelić, Brana, Škaro Bogojević, Sanja, Milivojević, Dušan, Ilić-Tomić, Tatjana, Lončarević, Branka, Beskoski, Vladimir, Maslak, Veselin, Guzik, Maciej, Makryniotis, Konstantinos, Taxeidis, George, Siaperas, Romanos, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Set of Small Molecule Polyurethane (PU) Model Substrates: Ecotoxicity Evaluation and Identification of PU Degrading Biocatalysts" in Catalysts, 13, no. 2 (2023):278,
https://doi.org/10.3390/catal13020278 . .

TY  - JOUR
AU  - Herrera, Diana A. Garza
AU  - Mojićević, Marija
AU  - Pantelić, Brana
AU  - Joshi, Akanksha
AU  - Collins, Catherine
AU  - Batista, Maria
AU  - Torres, Cristiana
AU  - Freitas, Filomena
AU  - Murray, Patrick
AU  - Nikodinović-Runić, Jasmina
AU  - Brennan Fournet, Margaret
PY  - 2023
UR  - https://www.mdpi.com/2076-2607/11/12/2914
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2225
AB  - The exposure of microorganisms to conventional plastics is a relatively recent occurrence, affording limited time for evolutionary adaptation. As part of the EU-funded project BioICEP, this study delves into the plastic degradation potential of microorganisms isolated from sites with prolonged plastic pollution, such as plastic-polluted forests, biopolymer-contaminated soil, oil-contaminated soil, municipal landfill, but also a distinctive soil sample with plastic pieces buried three decades ago. Additionally, samples from Arthropoda species were investigated. In total, 150 strains were isolated and screened for the ability to use plastic-related substrates (Impranil dispersions, polyethylene terephthalate, terephthalic acid, and bis(2-hydroxyethyl) terephthalate). Twenty isolates selected based on their ability to grow on various substrates were identified as Streptomyces, Bacillus, Enterococcus, and Pseudomonas spp. Morphological features were recorded, and the 16S rRNA sequence was employed to construct a phylogenetic tree. Subsequent assessments unveiled that 5 out of the 20 strains displayed the capability to produce polyhydroxyalkanoates, utilizing pre-treated post-consumer PET samples. With Priestia sp. DG69 and Neobacillus sp. DG40 emerging as the most successful producers (4.14% and 3.34% of PHA, respectively), these strains are poised for further utilization in upcycling purposes, laying the foundation for the development of sustainable strategies for plastic waste management.
T2  - Microorganisms
T2  - Microorganisms
T1  - Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential
IS  - 12
SP  - 2914
VL  - 11
DO  - 10.3390/microorganisms11122914
ER  - 

@article{
author = "Herrera, Diana A. Garza and Mojićević, Marija and Pantelić, Brana and Joshi, Akanksha and Collins, Catherine and Batista, Maria and Torres, Cristiana and Freitas, Filomena and Murray, Patrick and Nikodinović-Runić, Jasmina and Brennan Fournet, Margaret",
year = "2023",
abstract = "The exposure of microorganisms to conventional plastics is a relatively recent occurrence, affording limited time for evolutionary adaptation. As part of the EU-funded project BioICEP, this study delves into the plastic degradation potential of microorganisms isolated from sites with prolonged plastic pollution, such as plastic-polluted forests, biopolymer-contaminated soil, oil-contaminated soil, municipal landfill, but also a distinctive soil sample with plastic pieces buried three decades ago. Additionally, samples from Arthropoda species were investigated. In total, 150 strains were isolated and screened for the ability to use plastic-related substrates (Impranil dispersions, polyethylene terephthalate, terephthalic acid, and bis(2-hydroxyethyl) terephthalate). Twenty isolates selected based on their ability to grow on various substrates were identified as Streptomyces, Bacillus, Enterococcus, and Pseudomonas spp. Morphological features were recorded, and the 16S rRNA sequence was employed to construct a phylogenetic tree. Subsequent assessments unveiled that 5 out of the 20 strains displayed the capability to produce polyhydroxyalkanoates, utilizing pre-treated post-consumer PET samples. With Priestia sp. DG69 and Neobacillus sp. DG40 emerging as the most successful producers (4.14% and 3.34% of PHA, respectively), these strains are poised for further utilization in upcycling purposes, laying the foundation for the development of sustainable strategies for plastic waste management.",
journal = "Microorganisms, Microorganisms",
title = "Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential",
number = "12",
pages = "2914",
volume = "11",
doi = "10.3390/microorganisms11122914"
}

Herrera, D. A. G., Mojićević, M., Pantelić, B., Joshi, A., Collins, C., Batista, M., Torres, C., Freitas, F., Murray, P., Nikodinović-Runić, J.,& Brennan Fournet, M.. (2023). Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential. in Microorganisms, 11(12), 2914.
https://doi.org/10.3390/microorganisms11122914

Herrera DAG, Mojićević M, Pantelić B, Joshi A, Collins C, Batista M, Torres C, Freitas F, Murray P, Nikodinović-Runić J, Brennan Fournet M. Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential. in Microorganisms. 2023;11(12):2914.
doi:10.3390/microorganisms11122914 .

Herrera, Diana A. Garza, Mojićević, Marija, Pantelić, Brana, Joshi, Akanksha, Collins, Catherine, Batista, Maria, Torres, Cristiana, Freitas, Filomena, Murray, Patrick, Nikodinović-Runić, Jasmina, Brennan Fournet, Margaret, "Exploring Microorganisms from Plastic-Polluted Sites: Unveiling Plastic Degradation and PHA Production Potential" in Microorganisms, 11, no. 12 (2023):2914,
https://doi.org/10.3390/microorganisms11122914 . .

TY  - JOUR
AU  - Taxeidis, George
AU  - Nikolaivits, Efstratios
AU  - Siaperas, Romanos
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0269749123004621
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1797
AB  - The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.
T2  - Environmental Pollution
T1  - Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes
SP  - 121460
VL  - 325
DO  - 10.1016/j.envpol.2023.121460
ER  - 

@article{
author = "Taxeidis, George and Nikolaivits, Efstratios and Siaperas, Romanos and Gkountela, Christina and Vouyiouka, Stamatina and Pantelić, Brana and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2023",
abstract = "The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.",
journal = "Environmental Pollution",
title = "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes",
pages = "121460",
volume = "325",
doi = "10.1016/j.envpol.2023.121460"
}

Taxeidis, G., Nikolaivits, E., Siaperas, R., Gkountela, C., Vouyiouka, S., Pantelić, B., Nikodinović-Runić, J.,& Topakas, E.. (2023). Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution, 325, 121460.
https://doi.org/10.1016/j.envpol.2023.121460

Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelić B, Nikodinović-Runić J, Topakas E. Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution. 2023;325:121460.
doi:10.1016/j.envpol.2023.121460 .

Taxeidis, George, Nikolaivits, Efstratios, Siaperas, Romanos, Gkountela, Christina, Vouyiouka, Stamatina, Pantelić, Brana, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes" in Environmental Pollution, 325 (2023):121460,
https://doi.org/10.1016/j.envpol.2023.121460 . .

TY  - CONF
AU  - Lješević, M.
AU  - Lončarević, B.
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Pantelić, Brana
AU  - Gojgić-Cvijović, G.
AU  - Beškoski, V.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2173
AB  - Proizvodnja plastike i zamena staklene i keramičke ambalaže plastičnim materijalima
doveli su do nagomilavanja plastičnog otpada. Neophodno je naći povoljan sistem za
degradaciju plastičnog otpada, bez nastanka toksičnih produkata ili dodatnog zagađenja
životne sredine. Polietilen-tereftalat (PET) je jedan od najčešće proizvedenih plastičnih
polimera. Proizvodnja PET-a započinje esterifikacijom tereftalne kiseline i etilen glikola,
pri čemu nastaje bis-(2-hidroksietil)-tereftalat (BHET), koji se dalje polikondenzuje do
polimera. U poslednje vreme, BHET se često koristi kao model jedinjenje za identifikovanje
novih biokatalizatora za degradaciju PET-a [1,2].
Cilj ovog rada bio je ispitivanje mehanizma degradacije BHET-a pomoću
mikroorganizama.
U preliminarnom testu na čvrstim podlogama, kapacitet za degradaciju BHET-a je
testiran kod stotinak mikroorganizama, nakon čega su odabrani najefikasniji sojevi, koji su
identifikovani sekvenciranjem gena za 16s rRNK. Dalje, ispitivana je degradacija u tečnoj
podlozi gde je BHET bio glavni izvor ugljenika. Eksperiment je trajao 7 dana, a degradacija
je praćena nakon drugog, petog i sedmog dana upotrebom tečne hromatografije (HPLC).
Kao najefikasniji sojevi pokazali su se pripadnici roda Pseudomonas. Oni su u potpunosti
transformisali BHET do različitih intermedijera.
Rezultati su pokazali da ispitivani sojevi mogu da transformišu BHET, korišćenjem
najmanje dva različita puta, pa će se naredni eksperimenti usmeriti na identifikaciju
intermedijera degradacije. Takođe, radi optimizacije degradacije, ispitivaće se simbiotsko i
sinergističko dejstvo različitih konzorcijuma, kako bi se obezbedila potpuna degradacija
ovog model jedinjenja.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata
T1  - Microbial degradation of bis (2-hydroxyethyl) terephthalate
EP  - 44
SP  - 43
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2173
ER  - 

@conference{
author = "Lješević, M. and Lončarević, B. and Joksimović, K. and Žerađanin, A. and Pantelić, Brana and Gojgić-Cvijović, G. and Beškoski, V. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Proizvodnja plastike i zamena staklene i keramičke ambalaže plastičnim materijalima
doveli su do nagomilavanja plastičnog otpada. Neophodno je naći povoljan sistem za
degradaciju plastičnog otpada, bez nastanka toksičnih produkata ili dodatnog zagađenja
životne sredine. Polietilen-tereftalat (PET) je jedan od najčešće proizvedenih plastičnih
polimera. Proizvodnja PET-a započinje esterifikacijom tereftalne kiseline i etilen glikola,
pri čemu nastaje bis-(2-hidroksietil)-tereftalat (BHET), koji se dalje polikondenzuje do
polimera. U poslednje vreme, BHET se često koristi kao model jedinjenje za identifikovanje
novih biokatalizatora za degradaciju PET-a [1,2].
Cilj ovog rada bio je ispitivanje mehanizma degradacije BHET-a pomoću
mikroorganizama.
U preliminarnom testu na čvrstim podlogama, kapacitet za degradaciju BHET-a je
testiran kod stotinak mikroorganizama, nakon čega su odabrani najefikasniji sojevi, koji su
identifikovani sekvenciranjem gena za 16s rRNK. Dalje, ispitivana je degradacija u tečnoj
podlozi gde je BHET bio glavni izvor ugljenika. Eksperiment je trajao 7 dana, a degradacija
je praćena nakon drugog, petog i sedmog dana upotrebom tečne hromatografije (HPLC).
Kao najefikasniji sojevi pokazali su se pripadnici roda Pseudomonas. Oni su u potpunosti
transformisali BHET do različitih intermedijera.
Rezultati su pokazali da ispitivani sojevi mogu da transformišu BHET, korišćenjem
najmanje dva različita puta, pa će se naredni eksperimenti usmeriti na identifikaciju
intermedijera degradacije. Takođe, radi optimizacije degradacije, ispitivaće se simbiotsko i
sinergističko dejstvo različitih konzorcijuma, kako bi se obezbedila potpuna degradacija
ovog model jedinjenja.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata, Microbial degradation of bis (2-hydroxyethyl) terephthalate",
pages = "44-43",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2173"
}

Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Pantelić, B., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 43-44.
https://hdl.handle.net/21.15107/rcub_imagine_2173

Lješević M, Lončarević B, Joksimović K, Žerađanin A, Pantelić B, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:43-44.
https://hdl.handle.net/21.15107/rcub_imagine_2173 .

Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Pantelić, Brana, Gojgić-Cvijović, G., Beškoski, V., Nikodinović-Runić, Jasmina, "Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):43-44,
https://hdl.handle.net/21.15107/rcub_imagine_2173 .

TY  - JOUR
AU  - Nikolaivits, Efstratios
AU  - Pantelić, Brana
AU  - Azeem, Muhammad
AU  - Taxeidis, George
AU  - Babu, Ramesh
AU  - Topakas, Evangelos
AU  - Fournet, Margaret Brennan
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1479
AB  - Inspirational concepts, and the transfer of analogs from natural biology to science and engineering, has produced many excellent technologies to date, spanning vaccines to modern architectural feats. This review highlights that answers to the pressing global petroleum-based plastic waste challenges, can be found within the mechanics and mechanisms natural ecosystems. Here, a suite of technological and engineering approaches, which can be implemented to operate in tandem with nature's prescription for regenerative material circularity, is presented as a route to plastics sustainability. A number of mechanical/green chemical (pre)treatment methodologies, which simulate natural weathering and arthropodal dismantling activities are reviewed, including: mechanical milling, reactive extrusion, ultrasonic-, UV- and degradation using supercritical CO2. Akin to natural mechanical degradation, the purpose of the pretreatments is to render the plastic materials more amenable to microbial and biocatalytic activities, to yield effective depolymerization and (re)valorization. While biotechnological based degradation and depolymerization of both recalcitrant and bioplastics are at a relatively early stage of development, the potential for acceleration and expedition of valuable output monomers and oligomers yields is considerable. To date a limited number of independent mechano-green chemical approaches and a considerable and growing number of standalone enzymatic and microbial degradation studies have been reported. A convergent strategy, one which forges mechano-green chemical treatments together with the enzymatic and microbial actions, is largely lacking at this time. An overview of the reported microbial and enzymatic degradations of petroleum-based synthetic polymer plastics, specifically: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polystyrene (PS), polyethylene terephthalate (PET), polyurethanes (PU) and polycaprolactone (PCL) and selected prevalent bio-based or bio-polymers [polylactic acid (PLA), polyhydroxyalkanoates (PHAs) and polybutylene succinate (PBS)], is detailed. The harvesting of depolymerization products to produce new materials and higher-value products is also a key endeavor in effectively completing the circle for plastics. Our challenge is now to effectively combine and conjugate the requisite cross disciplinary approaches and progress the essential science and engineering technologies to categorically complete the life-cycle for plastics.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization
VL  - 9
DO  - 10.3389/fbioe.2021.696040
ER  - 

@article{
author = "Nikolaivits, Efstratios and Pantelić, Brana and Azeem, Muhammad and Taxeidis, George and Babu, Ramesh and Topakas, Evangelos and Fournet, Margaret Brennan and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Inspirational concepts, and the transfer of analogs from natural biology to science and engineering, has produced many excellent technologies to date, spanning vaccines to modern architectural feats. This review highlights that answers to the pressing global petroleum-based plastic waste challenges, can be found within the mechanics and mechanisms natural ecosystems. Here, a suite of technological and engineering approaches, which can be implemented to operate in tandem with nature's prescription for regenerative material circularity, is presented as a route to plastics sustainability. A number of mechanical/green chemical (pre)treatment methodologies, which simulate natural weathering and arthropodal dismantling activities are reviewed, including: mechanical milling, reactive extrusion, ultrasonic-, UV- and degradation using supercritical CO2. Akin to natural mechanical degradation, the purpose of the pretreatments is to render the plastic materials more amenable to microbial and biocatalytic activities, to yield effective depolymerization and (re)valorization. While biotechnological based degradation and depolymerization of both recalcitrant and bioplastics are at a relatively early stage of development, the potential for acceleration and expedition of valuable output monomers and oligomers yields is considerable. To date a limited number of independent mechano-green chemical approaches and a considerable and growing number of standalone enzymatic and microbial degradation studies have been reported. A convergent strategy, one which forges mechano-green chemical treatments together with the enzymatic and microbial actions, is largely lacking at this time. An overview of the reported microbial and enzymatic degradations of petroleum-based synthetic polymer plastics, specifically: low-density polyethylene (LDPE), high-density polyethylene (HDPE), polystyrene (PS), polyethylene terephthalate (PET), polyurethanes (PU) and polycaprolactone (PCL) and selected prevalent bio-based or bio-polymers [polylactic acid (PLA), polyhydroxyalkanoates (PHAs) and polybutylene succinate (PBS)], is detailed. The harvesting of depolymerization products to produce new materials and higher-value products is also a key endeavor in effectively completing the circle for plastics. Our challenge is now to effectively combine and conjugate the requisite cross disciplinary approaches and progress the essential science and engineering technologies to categorically complete the life-cycle for plastics.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization",
volume = "9",
doi = "10.3389/fbioe.2021.696040"
}

Nikolaivits, E., Pantelić, B., Azeem, M., Taxeidis, G., Babu, R., Topakas, E., Fournet, M. B.,& Nikodinović-Runić, J.. (2021). Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization. in Frontiers in Bioengineering and Biotechnology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fbioe.2021.696040

Nikolaivits E, Pantelić B, Azeem M, Taxeidis G, Babu R, Topakas E, Fournet MB, Nikodinović-Runić J. Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization. in Frontiers in Bioengineering and Biotechnology. 2021;9.
doi:10.3389/fbioe.2021.696040 .

Nikolaivits, Efstratios, Pantelić, Brana, Azeem, Muhammad, Taxeidis, George, Babu, Ramesh, Topakas, Evangelos, Fournet, Margaret Brennan, Nikodinović-Runić, Jasmina, "Progressing Plastics Circularity: A Review of Mechano-Biocatalytic Approaches for Waste Plastic (Re)valorization" in Frontiers in Bioengineering and Biotechnology, 9 (2021),
https://doi.org/10.3389/fbioe.2021.696040 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Ponjavić, Marijana
AU  - Janković, Vukašin
AU  - Aleksić, Ivana
AU  - Stevanović, Sanja
AU  - Murray, James
AU  - Fournet, Margaret Brennan
AU  - Nikodinović-Runić, Jasmina
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1470
AB  - Meeting the challenge of circularity for plastics requires amenability to repurposing post-use, as equivalent or upcycled products. In a compelling advancement, complete circularity for a biodegradable polyvinyl alcohol/thermoplastic starch (PVA/TPS) food packaging film was demonstrated by bioconversion to high-market-value biopigments and polyhydroxybutyrate (PHB) polyesters. The PVA/TPS film mechanical properties (tensile strength (sigma(u)), 22.2 & PLUSMN; 4.3 MPa; strain at break (epsilon(u)), 325 & PLUSMN; 73%; and Young's modulus (E), 53-250 MPa) compared closely with low-density polyethylene (LDPE) grades used for food packaging. Strong solubility of the PVA/TPS film in water was a pertinent feature, facilitating suitability as a carbon source for bioprocessing and microbial degradation. Biodegradability of the film with greater than 50% weight loss occurred within 30 days of incubation at 37 & DEG;C in a model compost. Up to 22% of the PVA/TPS film substrate conversion to biomass was achieved using three bacterial strains, Ralstonia eutropha H16 (Cupriavidus necator ATCC 17699), Streptomyces sp. JS520, and Bacillus subtilis ATCC6633. For the first time, production of the valuable biopigment (undecylprodigiosin) by Streptomyces sp. JS520 of 5.3 mg/mL and the production of PHB biopolymer at 7.8% of cell dry weight by Ralstonia eutropha H16 from this substrate were reported. This low-energy, low-carbon post-use PVA/TPS film upcycling model approach to plastic circularity demonstrates marked progress in the quest for sustainable and circular plastic solutions.
PB  - MDPI, Basel
T2  - Polymers
T1  - Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer
IS  - 21
VL  - 13
DO  - 10.3390/polym13213692
ER  - 

@article{
author = "Pantelić, Brana and Ponjavić, Marijana and Janković, Vukašin and Aleksić, Ivana and Stevanović, Sanja and Murray, James and Fournet, Margaret Brennan and Nikodinović-Runić, Jasmina",
year = "2021",
abstract = "Meeting the challenge of circularity for plastics requires amenability to repurposing post-use, as equivalent or upcycled products. In a compelling advancement, complete circularity for a biodegradable polyvinyl alcohol/thermoplastic starch (PVA/TPS) food packaging film was demonstrated by bioconversion to high-market-value biopigments and polyhydroxybutyrate (PHB) polyesters. The PVA/TPS film mechanical properties (tensile strength (sigma(u)), 22.2 & PLUSMN; 4.3 MPa; strain at break (epsilon(u)), 325 & PLUSMN; 73%; and Young's modulus (E), 53-250 MPa) compared closely with low-density polyethylene (LDPE) grades used for food packaging. Strong solubility of the PVA/TPS film in water was a pertinent feature, facilitating suitability as a carbon source for bioprocessing and microbial degradation. Biodegradability of the film with greater than 50% weight loss occurred within 30 days of incubation at 37 & DEG;C in a model compost. Up to 22% of the PVA/TPS film substrate conversion to biomass was achieved using three bacterial strains, Ralstonia eutropha H16 (Cupriavidus necator ATCC 17699), Streptomyces sp. JS520, and Bacillus subtilis ATCC6633. For the first time, production of the valuable biopigment (undecylprodigiosin) by Streptomyces sp. JS520 of 5.3 mg/mL and the production of PHB biopolymer at 7.8% of cell dry weight by Ralstonia eutropha H16 from this substrate were reported. This low-energy, low-carbon post-use PVA/TPS film upcycling model approach to plastic circularity demonstrates marked progress in the quest for sustainable and circular plastic solutions.",
publisher = "MDPI, Basel",
journal = "Polymers",
title = "Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer",
number = "21",
volume = "13",
doi = "10.3390/polym13213692"
}

Pantelić, B., Ponjavić, M., Janković, V., Aleksić, I., Stevanović, S., Murray, J., Fournet, M. B.,& Nikodinović-Runić, J.. (2021). Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer. in Polymers
MDPI, Basel., 13(21).
https://doi.org/10.3390/polym13213692

Pantelić B, Ponjavić M, Janković V, Aleksić I, Stevanović S, Murray J, Fournet MB, Nikodinović-Runić J. Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer. in Polymers. 2021;13(21).
doi:10.3390/polym13213692 .

Pantelić, Brana, Ponjavić, Marijana, Janković, Vukašin, Aleksić, Ivana, Stevanović, Sanja, Murray, James, Fournet, Margaret Brennan, Nikodinović-Runić, Jasmina, "Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer" in Polymers, 13, no. 21 (2021),
https://doi.org/10.3390/polym13213692 . .