TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 

@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}

Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364

Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Milošević, Mia
AU  - Petrović, Jelena
AU  - Menshikh, Ksenia
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Janković, Radmila
AU  - Cochis, Andrea
AU  - Bella, Elena Della
AU  - Stoddart, Martin
AU  - Rimondini, Lia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1261
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2363
AB  - Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.
C3  - Hemijska industrija (Chemical Industry)
T1  - A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research
EP  - 19
IS  - 1S
SP  - 19
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2363
ER  - 

@conference{
author = "Banićević, Ivana and Milošević, Mia and Petrović, Jelena and Menshikh, Ksenia and Milivojević, Milena and Stevanović, Milena and Janković, Radmila and Cochis, Andrea and Bella, Elena Della and Stoddart, Martin and Rimondini, Lia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2024",
abstract = "Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research",
pages = "19-19",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2363"
}

Banićević, I., Milošević, M., Petrović, J., Menshikh, K., Milivojević, M., Stevanović, M., Janković, R., Cochis, A., Bella, E. D., Stoddart, M., Rimondini, L., Stojkovska, J.,& Obradović, B.. (2024). A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry), 78(1S), 19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363

Banićević I, Milošević M, Petrović J, Menshikh K, Milivojević M, Stevanović M, Janković R, Cochis A, Bella ED, Stoddart M, Rimondini L, Stojkovska J, Obradović B. A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry). 2024;78(1S):19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

Banićević, Ivana, Milošević, Mia, Petrović, Jelena, Menshikh, Ksenia, Milivojević, Milena, Stevanović, Milena, Janković, Radmila, Cochis, Andrea, Bella, Elena Della, Stoddart, Martin, Rimondini, Lia, Stojkovska, Jasmina, Obradović, Bojana, "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):19-19,
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

TY  - CONF
AU  - Bojić, Luka
AU  - Pejić, Jelena
AU  - Stojkovska, Jasmina
AU  - Stevanović, Milena
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Milivojević, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1262
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2366
AB  - Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.
C3  - Hemijska industrija (Chemical Industry)
T1  - Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems
EP  - 20
IS  - 1S
SP  - 20
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2366
ER  - 

@conference{
author = "Bojić, Luka and Pejić, Jelena and Stojkovska, Jasmina and Stevanović, Milena and Medić, Aleksandra and Petrović, Isidora and Milivojević, Milena",
year = "2024",
abstract = "Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems",
pages = "20-20",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2366"
}

Bojić, L., Pejić, J., Stojkovska, J., Stevanović, M., Medić, A., Petrović, I.,& Milivojević, M.. (2024). Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry), 78(1S), 20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366

Bojić L, Pejić J, Stojkovska J, Stevanović M, Medić A, Petrović I, Milivojević M. Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry). 2024;78(1S):20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

Bojić, Luka, Pejić, Jelena, Stojkovska, Jasmina, Stevanović, Milena, Medić, Aleksandra, Petrović, Isidora, Milivojević, Milena, "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):20-20,
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

TY  - CONF
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Janković, Radmila
AU  - Dragoj, Miodrag
AU  - Jančić, Ivan
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1265
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2365
AB  - Development of novel, effective, and safe anti-tumor drugs is still a slow and cumbersome process, which is often attributed to weaknesses of current preclinical assays and low correlation of the preclinical in vitro and in vivo data with the results obtained in clinical trials. Consequently, there is a clear need for development of more reliable in vitro three dimensional (3D) tumor models, which will capture key features of the in vivo tumor cell microenvironment and provide drug testing results relevant for human patients. The aim of the project “Biomimetic tumor engineering to enhance drug discovery – BioengineeredTumor” funded by the Science Fund of the Republic of Serbia is to develop 2 novel, simple and robust 3D models for cultures of carcinoma and osteosarcoma cells by applying systematic and integrated methodology to comprehensively define the key model components. In specific, the aim is to use different human and animal cancer cell lines in conjunction with alginate-based biomaterials as artificial extracellular matrices imitating tumor environments and to cultivate the obtained constructs in perfusion bioreactors providing enhanced transport of nutrients, gases and biochemical signals to the cells as well as adequate levels of hydrodynamic shear stresses. Thus, the strategic goal is to establish an adaptable platform suited to the use by scientists without technical expertise for long-term in vitro studies of cancer cells for applications in anti-cancer drug discovery and validation, development of personalized medical treatments, and cancer research.
C3  - Hemijska industrija (Chemical Industry)
T1  - Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor
EP  - 22
IS  - 1S
SP  - 22
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2365
ER  - 

@conference{
author = "Obradović, Bojana and Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Janković, Radmila and Dragoj, Miodrag and Jančić, Ivan",
year = "2024",
abstract = "Development of novel, effective, and safe anti-tumor drugs is still a slow and cumbersome process, which is often attributed to weaknesses of current preclinical assays and low correlation of the preclinical in vitro and in vivo data with the results obtained in clinical trials. Consequently, there is a clear need for development of more reliable in vitro three dimensional (3D) tumor models, which will capture key features of the in vivo tumor cell microenvironment and provide drug testing results relevant for human patients. The aim of the project “Biomimetic tumor engineering to enhance drug discovery – BioengineeredTumor” funded by the Science Fund of the Republic of Serbia is to develop 2 novel, simple and robust 3D models for cultures of carcinoma and osteosarcoma cells by applying systematic and integrated methodology to comprehensively define the key model components. In specific, the aim is to use different human and animal cancer cell lines in conjunction with alginate-based biomaterials as artificial extracellular matrices imitating tumor environments and to cultivate the obtained constructs in perfusion bioreactors providing enhanced transport of nutrients, gases and biochemical signals to the cells as well as adequate levels of hydrodynamic shear stresses. Thus, the strategic goal is to establish an adaptable platform suited to the use by scientists without technical expertise for long-term in vitro studies of cancer cells for applications in anti-cancer drug discovery and validation, development of personalized medical treatments, and cancer research.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor",
pages = "22-22",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2365"
}

Obradović, B., Stojkovska, J., Zvicer, J., Milivojević, M., Janković, R., Dragoj, M.,& Jančić, I.. (2024). Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor. in Hemijska industrija (Chemical Industry), 78(1S), 22-22.
https://hdl.handle.net/21.15107/rcub_imagine_2365

Obradović B, Stojkovska J, Zvicer J, Milivojević M, Janković R, Dragoj M, Jančić I. Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor. in Hemijska industrija (Chemical Industry). 2024;78(1S):22-22.
https://hdl.handle.net/21.15107/rcub_imagine_2365 .

Obradović, Bojana, Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Janković, Radmila, Dragoj, Miodrag, Jančić, Ivan, "Biomimetic tumor engineering to enhance drug discovery - BioengineeredTumor" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):22-22,
https://hdl.handle.net/21.15107/rcub_imagine_2365 .

TY  - CONF
AU  - Pejić, Jelena
AU  - Mojsin, Marija
AU  - Stojkovska, Jasmina
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2151
AB  - Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds
EP  - 113
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2151
ER  - 

@conference{
author = "Pejić, Jelena and Mojsin, Marija and Stojkovska, Jasmina and Medić, Aleksandra and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana and Milivojević, Milena",
year = "2023",
abstract = "Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2151"
}

Pejić, J., Mojsin, M., Stojkovska, J., Medić, A., Petrović, I., Stevanović, M., Obradović, B.,& Milivojević, M.. (2023). Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151

Pejić J, Mojsin M, Stojkovska J, Medić A, Petrović I, Stevanović M, Obradović B, Milivojević M. Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

Pejić, Jelena, Mojsin, Marija, Stojkovska, Jasmina, Medić, Aleksandra, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, Milivojević, Milena, "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):113-113,
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

TY  - CONF
AU  - Bojić, Luka
AU  - Schwirtlich, Marija
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Balint, Vanda
AU  - Stevanović, Milena
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2133
AB  - Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro
EP  - 143
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2133
ER  - 

@conference{
author = "Bojić, Luka and Schwirtlich, Marija and Lazić, Stefan and Stanisavljević Ninković, Danijela and Balint, Vanda and Stevanović, Milena and Milivojević, Milena",
year = "2023",
abstract = "Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2133"
}

Bojić, L., Schwirtlich, M., Lazić, S., Stanisavljević Ninković, D., Balint, V., Stevanović, M.,& Milivojević, M.. (2023). The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2133

Bojić L, Schwirtlich M, Lazić S, Stanisavljević Ninković D, Balint V, Stevanović M, Milivojević M. The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2133 .

Bojić, Luka, Schwirtlich, Marija, Lazić, Stefan, Stanisavljević Ninković, Danijela, Balint, Vanda, Stevanović, Milena, Milivojević, Milena, "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):143-143,
https://hdl.handle.net/21.15107/rcub_imagine_2133 .

TY  - CONF
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Aleksandra, Medić
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Erceg, Slaven
AU  - Stevanović, Milena
AU  - Švirtlih, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2287
AB  - Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2287
ER  - 

@conference{
author = "Lazić, Stefan and Stanisavljević Ninković, Danijela and Petrović, Isidora and Aleksandra, Medić and Milivojević, Milena and Bojić, Luka and Erceg, Slaven and Stevanović, Milena and Švirtlih, Marija",
year = "2023",
abstract = "Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2287"
}

Lazić, S., Stanisavljević Ninković, D., Petrović, I., Aleksandra, M., Milivojević, M., Bojić, L., Erceg, S., Stevanović, M.,& Švirtlih, M.. (2023). Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287

Lazić S, Stanisavljević Ninković D, Petrović I, Aleksandra M, Milivojević M, Bojić L, Erceg S, Stevanović M, Švirtlih M. Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society. 2023;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

Lazić, Stefan, Stanisavljević Ninković, Danijela, Petrović, Isidora, Aleksandra, Medić, Milivojević, Milena, Bojić, Luka, Erceg, Slaven, Stevanović, Milena, Švirtlih, Marija, "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21" in 8th Congress of the Serbian Neuroscience Society (2023):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2181
AB  - Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.
PB  - Springer Nature
C3  - European Journal of Human Genetics
T1  - Detection rate of 22q11.2 microdeletion using strict diagnostic criteria
EP  - 240
IS  - Suppl 1
SP  - 240
VL  - 31
DO  - 10.1038/s41431-023-01339-3
ER  - 

@conference{
author = "Drakulić, Danijela and Cuturilo, Goran and Jovanović, Ida and Krstić, Aleksandar and Milivojević, Milena and Stevanović, Milena",
year = "2023",
abstract = "Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.",
publisher = "Springer Nature",
journal = "European Journal of Human Genetics",
title = "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria",
pages = "240-240",
number = "Suppl 1",
volume = "31",
doi = "10.1038/s41431-023-01339-3"
}

Drakulić, D., Cuturilo, G., Jovanović, I., Krstić, A., Milivojević, M.,& Stevanović, M.. (2023). Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics
Springer Nature., 31(Suppl 1), 240-240.
https://doi.org/10.1038/s41431-023-01339-3

Drakulić D, Cuturilo G, Jovanović I, Krstić A, Milivojević M, Stevanović M. Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics. 2023;31(Suppl 1):240-240.
doi:10.1038/s41431-023-01339-3 .

Drakulić, Danijela, Cuturilo, Goran, Jovanović, Ida, Krstić, Aleksandar, Milivojević, Milena, Stevanović, Milena, "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria" in European Journal of Human Genetics, 31, no. Suppl 1 (2023):240-240,
https://doi.org/10.1038/s41431-023-01339-3 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
AU  - Petrović, Isidora
AU  - Drakulić, Danijela
AU  - Milivojević, Milena
AU  - Mojsin, Marija
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/7/6392
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1890
AB  - Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma
IS  - 7
SP  - 6392
VL  - 24
DO  - 10.3390/ijms24076392
ER  - 

@article{
author = "Stevanović, Milena and Kovačević-Grujičić, Nataša and Petrović, Isidora and Drakulić, Danijela and Milivojević, Milena and Mojsin, Marija",
year = "2023",
abstract = "Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma",
number = "7",
pages = "6392",
volume = "24",
doi = "10.3390/ijms24076392"
}

Stevanović, M., Kovačević-Grujičić, N., Petrović, I., Drakulić, D., Milivojević, M.,& Mojsin, M.. (2023). Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences, 24(7), 6392.
https://doi.org/10.3390/ijms24076392

Stevanović M, Kovačević-Grujičić N, Petrović I, Drakulić D, Milivojević M, Mojsin M. Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences. 2023;24(7):6392.
doi:10.3390/ijms24076392 .

Stevanović, Milena, Kovačević-Grujičić, Nataša, Petrović, Isidora, Drakulić, Danijela, Milivojević, Milena, Mojsin, Marija, "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma" in International Journal of Molecular Sciences, 24, no. 7 (2023):6392,
https://doi.org/10.3390/ijms24076392 . .

TY  - JOUR
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1594
AB  - A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mu m s(-1) induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as similar to 67 mPa. On the contrary, similar velocity (100 mu m s(-1)) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of similar to 10 nm s(-1)). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of similar to 10(-)(19)-10(-11) m(2) s(-)(1) are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (similar to 10(-1)(9) m(2) s(-1)) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.
PB  - Savez hemijskih inženjera, Beograd
T2  - Chemical Industry & Chemical Engineering Quarterly
T1  - Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations
EP  - 223
IS  - 3
SP  - 211
VL  - 28
DO  - 10.2298/CICEQ210607033R
ER  - 

@article{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
abstract = "A multidisciplinary approach based on experiments and mathematical modeling was used in biomimetic system development for three-dimensional (3D) cultures of cancer cells. Specifically, two cancer cell lines, human embryonic teratocarcinoma NT2/D1 and rat glioma C6, were immobilized in alginate microbeads and microfibers, respectively, and cultured under static and flow conditions in perfusion bioreactors. At the same time, chemical engineering methods were applied to explain the obtained results. The superficial medium velocity of 80 mu m s(-1) induced lower viability of NT2/D1 cells in superficial microbead zones, implying adverse effects of fluid shear stresses estimated as similar to 67 mPa. On the contrary, similar velocity (100 mu m s(-1)) enhanced the proliferation of C6 glioma cells within microfibers compared to static controls. An additional study of silver release from nanocomposite Ag/honey/alginate microfibers under perfusion indicated that the medium partially flows through the hydrogel (interstitial velocity of similar to 10 nm s(-1)). Thus, a diffusion-advection-reaction model described the mass transport to immobilized cells within microfibers. Substances with diffusion coefficients of similar to 10(-)(19)-10(-11) m(2) s(-)(1) are sufficiently supplied by diffusion only, while those with significantly lower diffusivities (similar to 10(-1)(9) m(2) s(-1)) require additional convective transport. The present study demonstrates the selection and contribution of chemical engineering methods in tumor model system development.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Chemical Industry & Chemical Engineering Quarterly",
title = "Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations",
pages = "223-211",
number = "3",
volume = "28",
doi = "10.2298/CICEQ210607033R"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations. in Chemical Industry & Chemical Engineering Quarterly
Savez hemijskih inženjera, Beograd., 28(3), 211-223.
https://doi.org/10.2298/CICEQ210607033R

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations. in Chemical Industry & Chemical Engineering Quarterly. 2022;28(3):211-223.
doi:10.2298/CICEQ210607033R .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Chemical engineering methods in analyses of 3d cancer cell cultures: hydrodynamic and mass transport considerations" in Chemical Industry & Chemical Engineering Quarterly, 28, no. 3 (2022):211-223,
https://doi.org/10.2298/CICEQ210607033R . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Petrović, Isidora
AU  - Mojsin, Marija
AU  - Milivojević, Milena
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1519
AB  - Glioblastoma (GBM) is the most common and highly lethal type of brain tumor, with poor survival despite advances in understanding its complexity. After current standard therapeutic treatment, including tumor resection, radiotherapy and concomitant chemotherapy with temozolomide, the median overall survival of patients with this type of tumor is less than 15 months. Thus, there is an urgent need for new insights into GBM molecular characteristics and progress in targeted therapy in order to improve clinical outcomes. The literature data revealed that a number of different signaling pathways are dysregulated in GBM. In this review, we intended to summarize and discuss current literature data and therapeutic modalities focused on targeting dysregulated signaling pathways in GBM. A better understanding of opportunities for targeting signaling pathways that influences malignant behavior of GBM cells might open the way for the development of novel GBM-targeted therapies.
PB  - MDPI, Basel
T2  - Cells
T1  - Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma
IS  - 16
VL  - 11
DO  - 10.3390/cells11162530
ER  - 

@article{
author = "Drakulić, Danijela and Schwirtlich, Marija and Petrović, Isidora and Mojsin, Marija and Milivojević, Milena and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2022",
abstract = "Glioblastoma (GBM) is the most common and highly lethal type of brain tumor, with poor survival despite advances in understanding its complexity. After current standard therapeutic treatment, including tumor resection, radiotherapy and concomitant chemotherapy with temozolomide, the median overall survival of patients with this type of tumor is less than 15 months. Thus, there is an urgent need for new insights into GBM molecular characteristics and progress in targeted therapy in order to improve clinical outcomes. The literature data revealed that a number of different signaling pathways are dysregulated in GBM. In this review, we intended to summarize and discuss current literature data and therapeutic modalities focused on targeting dysregulated signaling pathways in GBM. A better understanding of opportunities for targeting signaling pathways that influences malignant behavior of GBM cells might open the way for the development of novel GBM-targeted therapies.",
publisher = "MDPI, Basel",
journal = "Cells",
title = "Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma",
number = "16",
volume = "11",
doi = "10.3390/cells11162530"
}

Drakulić, D., Schwirtlich, M., Petrović, I., Mojsin, M., Milivojević, M., Kovačević Grujičić, N.,& Stevanović, M.. (2022). Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma. in Cells
MDPI, Basel., 11(16).
https://doi.org/10.3390/cells11162530

Drakulić D, Schwirtlich M, Petrović I, Mojsin M, Milivojević M, Kovačević Grujičić N, Stevanović M. Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma. in Cells. 2022;11(16).
doi:10.3390/cells11162530 .

Drakulić, Danijela, Schwirtlich, Marija, Petrović, Isidora, Mojsin, Marija, Milivojević, Milena, Kovačević Grujičić, Nataša, Stevanović, Milena, "Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma" in Cells, 11, no. 16 (2022),
https://doi.org/10.3390/cells11162530 . .

TY  - CONF
AU  - Radonjić, Mia
AU  - Petrović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1586
PB  - Mary Ann Liebert, Inc, New Rochelle
C3  - Tissue Engineering Part A
T1  - Optimization of 3d cancer cell culture conditions by application of chemical engineering principles
EP  - S395
SP  - S395
VL  - 28
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1586
ER  - 

@conference{
author = "Radonjić, Mia and Petrović, Jelena and Milivojević, Milena and Stevanović, Milena and Stojkovska, Jasmina and Obradović, Bojana",
year = "2022",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Tissue Engineering Part A",
title = "Optimization of 3d cancer cell culture conditions by application of chemical engineering principles",
pages = "S395-S395",
volume = "28",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1586"
}

Radonjić, M., Petrović, J., Milivojević, M., Stevanović, M., Stojkovska, J.,& Obradović, B.. (2022). Optimization of 3d cancer cell culture conditions by application of chemical engineering principles. in Tissue Engineering Part A
Mary Ann Liebert, Inc, New Rochelle., 28, S395-S395.
https://hdl.handle.net/21.15107/rcub_imagine_1586

Radonjić M, Petrović J, Milivojević M, Stevanović M, Stojkovska J, Obradović B. Optimization of 3d cancer cell culture conditions by application of chemical engineering principles. in Tissue Engineering Part A. 2022;28:S395-S395.
https://hdl.handle.net/21.15107/rcub_imagine_1586 .

Radonjić, Mia, Petrović, Jelena, Milivojević, Milena, Stevanović, Milena, Stojkovska, Jasmina, Obradović, Bojana, "Optimization of 3d cancer cell culture conditions by application of chemical engineering principles" in Tissue Engineering Part A, 28 (2022):S395-S395,
https://hdl.handle.net/21.15107/rcub_imagine_1586 .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Arsenijević, Ana
AU  - Lazić, Andrijana
AU  - Kovačević Grujičić, Nataša
AU  - Schwirtlich, Marija
AU  - Popović, Jelena
AU  - Stevanović, Milena
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1505
AB  - Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.
PB  - Tech Science Press, Henderson
T2  - Biocell
T1  - Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells
EP  - 1367
IS  - 5
SP  - 1355
VL  - 45
DO  - 10.32604/biocell.2021.015817
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Arsenijević, Ana and Lazić, Andrijana and Kovačević Grujičić, Nataša and Schwirtlich, Marija and Popović, Jelena and Stevanović, Milena",
year = "2021",
abstract = "Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.",
publisher = "Tech Science Press, Henderson",
journal = "Biocell",
title = "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells",
pages = "1367-1355",
number = "5",
volume = "45",
doi = "10.32604/biocell.2021.015817"
}

Petrović, I., Milivojević, M., Arsenijević, A., Lazić, A., Kovačević Grujičić, N., Schwirtlich, M., Popović, J.,& Stevanović, M.. (2021). Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell
Tech Science Press, Henderson., 45(5), 1355-1367.
https://doi.org/10.32604/biocell.2021.015817

Petrović I, Milivojević M, Arsenijević A, Lazić A, Kovačević Grujičić N, Schwirtlich M, Popović J, Stevanović M. Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell. 2021;45(5):1355-1367.
doi:10.32604/biocell.2021.015817 .

Petrović, Isidora, Milivojević, Milena, Arsenijević, Ana, Lazić, Andrijana, Kovačević Grujičić, Nataša, Schwirtlich, Marija, Popović, Jelena, Stevanović, Milena, "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells" in Biocell, 45, no. 5 (2021):1355-1367,
https://doi.org/10.32604/biocell.2021.015817 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Milivojević, Milena
AU  - Drakulić, Danijela
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1468
AB  - Glioblastoma (GBM) is the most common, most aggressive and deadliest brain tumor. Recently, remarkable progress has been made towards understanding the cellular and molecular biology of gliomas. GBM tumor initiation, progression and relapse as well as resistance to treatments are associated with glioma stem cells (GSCs). GSCs exhibit a high proliferation rate and self-renewal capacity and the ability to differentiate into diverse cell types, generating a range of distinct cell types within the tumor, leading to cellular heterogeneity. GBM tumors may contain different subsets of GSCs, and some of them may adopt a quiescent state that protects them against chemotherapy and radiotherapy. GSCs enriched in recurrent gliomas acquire more aggressive and therapy-resistant properties, making them more malignant, able to rapidly spread. The impact of SOX transcription factors (TFs) on brain tumors has been extensively studied in the last decade. Almost all SOX genes are expressed in GBM, and their expression levels are associated with patient prognosis and survival. Numerous SOX TFs are involved in the maintenance of the stemness of GSCs or play a role in the initiation of GSC differentiation. The fine-tuning of SOX gene expression levels controls the balance between cell stemness and differentiation. Therefore, innovative therapies targeting SOX TFs are emerging as promising tools for combatting GBM. Combatting GBM has been a demanding and challenging goal for decades. The current therapeutic strategies have not yet provided a cure for GBM and have only resulted in a slight improvement in patient survival. Novel approaches will require the fine adjustment of multimodal therapeutic strategies that simultaneously target numerous hallmarks of cancer cells to win the battle against GBM.
PB  - Baishideng Publishing Group Inc, Pleasanton
T2  - World Journal of Stem Cells
T1  - SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation
EP  - 1445
IS  - 10
SP  - 1417
VL  - 13
DO  - 10.4252/wjsc.v13.i10.1417
ER  - 

@article{
author = "Stevanović, Milena and Kovačević Grujičić, Nataša and Mojsin, Marija and Milivojević, Milena and Drakulić, Danijela",
year = "2021",
abstract = "Glioblastoma (GBM) is the most common, most aggressive and deadliest brain tumor. Recently, remarkable progress has been made towards understanding the cellular and molecular biology of gliomas. GBM tumor initiation, progression and relapse as well as resistance to treatments are associated with glioma stem cells (GSCs). GSCs exhibit a high proliferation rate and self-renewal capacity and the ability to differentiate into diverse cell types, generating a range of distinct cell types within the tumor, leading to cellular heterogeneity. GBM tumors may contain different subsets of GSCs, and some of them may adopt a quiescent state that protects them against chemotherapy and radiotherapy. GSCs enriched in recurrent gliomas acquire more aggressive and therapy-resistant properties, making them more malignant, able to rapidly spread. The impact of SOX transcription factors (TFs) on brain tumors has been extensively studied in the last decade. Almost all SOX genes are expressed in GBM, and their expression levels are associated with patient prognosis and survival. Numerous SOX TFs are involved in the maintenance of the stemness of GSCs or play a role in the initiation of GSC differentiation. The fine-tuning of SOX gene expression levels controls the balance between cell stemness and differentiation. Therefore, innovative therapies targeting SOX TFs are emerging as promising tools for combatting GBM. Combatting GBM has been a demanding and challenging goal for decades. The current therapeutic strategies have not yet provided a cure for GBM and have only resulted in a slight improvement in patient survival. Novel approaches will require the fine adjustment of multimodal therapeutic strategies that simultaneously target numerous hallmarks of cancer cells to win the battle against GBM.",
publisher = "Baishideng Publishing Group Inc, Pleasanton",
journal = "World Journal of Stem Cells",
title = "SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation",
pages = "1445-1417",
number = "10",
volume = "13",
doi = "10.4252/wjsc.v13.i10.1417"
}

Stevanović, M., Kovačević Grujičić, N., Mojsin, M., Milivojević, M.,& Drakulić, D.. (2021). SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation. in World Journal of Stem Cells
Baishideng Publishing Group Inc, Pleasanton., 13(10), 1417-1445.
https://doi.org/10.4252/wjsc.v13.i10.1417

Stevanović M, Kovačević Grujičić N, Mojsin M, Milivojević M, Drakulić D. SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation. in World Journal of Stem Cells. 2021;13(10):1417-1445.
doi:10.4252/wjsc.v13.i10.1417 .

Stevanović, Milena, Kovačević Grujičić, Nataša, Mojsin, Marija, Milivojević, Milena, Drakulić, Danijela, "SOX transcription factors and glioma stem cells: Choosing between stemness and differentiation" in World Journal of Stem Cells, 13, no. 10 (2021):1417-1445,
https://doi.org/10.4252/wjsc.v13.i10.1417 . .

TY  - JOUR
AU  - Miletić, Aleksandra
AU  - Ruml Stojanović, Jelena
AU  - Parezanović, Vojislav
AU  - Rsovac, Snežana
AU  - Drakulić, Danijela
AU  - Soldatović, Ivan
AU  - Mijović, Marija
AU  - Bosankić, Brankica
AU  - Petrović, Hristina
AU  - Borlja, Nikola
AU  - Milivojević, Milena
AU  - Marjanović, Ana
AU  - Branković, Marija
AU  - Cuturilo, Goran
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1414
AB  - Rapid and efficient diagnostics is crucial for newborns with congenital heart defects (CHD) in intensive care unit (ICU) but is often challenging. Given that genetic factors play a role in 20-30% cases of CHD, it is likely that genetic tests could improve both its speed and efficiency. We aimed to analyze the utility of rapid and cost-effective multiplex ligation dependent probe amplification analysis (MLPA) for chromosomal analysis in newborns with critical CHD. One hundred consecutive newborns admitted with critical CHD to the ICU were included in the study. Those with normal MLPA findings were further tested by chromosomal microarray and clinical exome sequencing. Overall, pathogenic/likely pathogenic variants were determined in ten (10%) newborns by MLPA, three (3%) by chromosomal microarray, and three (3%) by clinical exome sequencing. The most common variant detected was deletion of 22q11.2 region. Conclusion: MLPA is fast and cost-effective analysis that could be used as the first-tier test in newborns with critical CHD admitted to the ICU. What is Known: center dot MLPA is an established method for chromosome analysis in patients with CHD, but detection rate in newborns with critical CHD is unknown. What is New: center dot Study suggests that detection rate of casual variants using MLPA in newborns with critical CHD is 10%.
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - Genetic evaluation of newborns with critical congenital heart defects admitted to the intensive care unit
EP  - 3227
IS  - 10
SP  - 3219
VL  - 180
DO  - 10.1007/s00431-021-04097-w
ER  - 

@article{
author = "Miletić, Aleksandra and Ruml Stojanović, Jelena and Parezanović, Vojislav and Rsovac, Snežana and Drakulić, Danijela and Soldatović, Ivan and Mijović, Marija and Bosankić, Brankica and Petrović, Hristina and Borlja, Nikola and Milivojević, Milena and Marjanović, Ana and Branković, Marija and Cuturilo, Goran",
year = "2021",
abstract = "Rapid and efficient diagnostics is crucial for newborns with congenital heart defects (CHD) in intensive care unit (ICU) but is often challenging. Given that genetic factors play a role in 20-30% cases of CHD, it is likely that genetic tests could improve both its speed and efficiency. We aimed to analyze the utility of rapid and cost-effective multiplex ligation dependent probe amplification analysis (MLPA) for chromosomal analysis in newborns with critical CHD. One hundred consecutive newborns admitted with critical CHD to the ICU were included in the study. Those with normal MLPA findings were further tested by chromosomal microarray and clinical exome sequencing. Overall, pathogenic/likely pathogenic variants were determined in ten (10%) newborns by MLPA, three (3%) by chromosomal microarray, and three (3%) by clinical exome sequencing. The most common variant detected was deletion of 22q11.2 region. Conclusion: MLPA is fast and cost-effective analysis that could be used as the first-tier test in newborns with critical CHD admitted to the ICU. What is Known: center dot MLPA is an established method for chromosome analysis in patients with CHD, but detection rate in newborns with critical CHD is unknown. What is New: center dot Study suggests that detection rate of casual variants using MLPA in newborns with critical CHD is 10%.",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "Genetic evaluation of newborns with critical congenital heart defects admitted to the intensive care unit",
pages = "3227-3219",
number = "10",
volume = "180",
doi = "10.1007/s00431-021-04097-w"
}

Miletić, A., Ruml Stojanović, J., Parezanović, V., Rsovac, S., Drakulić, D., Soldatović, I., Mijović, M., Bosankić, B., Petrović, H., Borlja, N., Milivojević, M., Marjanović, A., Branković, M.,& Cuturilo, G.. (2021). Genetic evaluation of newborns with critical congenital heart defects admitted to the intensive care unit. in European Journal of Pediatrics
Springer, New York., 180(10), 3219-3227.
https://doi.org/10.1007/s00431-021-04097-w

Miletić A, Ruml Stojanović J, Parezanović V, Rsovac S, Drakulić D, Soldatović I, Mijović M, Bosankić B, Petrović H, Borlja N, Milivojević M, Marjanović A, Branković M, Cuturilo G. Genetic evaluation of newborns with critical congenital heart defects admitted to the intensive care unit. in European Journal of Pediatrics. 2021;180(10):3219-3227.
doi:10.1007/s00431-021-04097-w .

Miletić, Aleksandra, Ruml Stojanović, Jelena, Parezanović, Vojislav, Rsovac, Snežana, Drakulić, Danijela, Soldatović, Ivan, Mijović, Marija, Bosankić, Brankica, Petrović, Hristina, Borlja, Nikola, Milivojević, Milena, Marjanović, Ana, Branković, Marija, Cuturilo, Goran, "Genetic evaluation of newborns with critical congenital heart defects admitted to the intensive care unit" in European Journal of Pediatrics, 180, no. 10 (2021):3219-3227,
https://doi.org/10.1007/s00431-021-04097-w . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1363
AB  - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Validation of a novel perfusion bioreactor system in cancer research
EP  - 196
IS  - 3
SP  - 187
VL  - 74
DO  - 10.2298/HEMIND200329015S
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana",
year = "2020",
abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Validation of a novel perfusion bioreactor system in cancer research",
pages = "196-187",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200329015S"
}

Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 74(3), 187-196.
https://doi.org/10.2298/HEMIND200329015S

Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska Industrija. 2020;74(3):187-196.
doi:10.2298/HEMIND200329015S .

Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska Industrija, 74, no. 3 (2020):187-196,
https://doi.org/10.2298/HEMIND200329015S . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Đukić, Milan
AU  - Skorić, Dejan
AU  - Mijović, Marija
AU  - Stefanović, Igor
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/961
AB  - Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries.
PB  - Springer India
T2  - Indian Pediatrics
T1  - Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia
EP  - 789
IS  - 9
SP  - 786
VL  - 53
DO  - 10.1007/s13312-016-0931-z
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Jovanović, Ida and Krstić, Aleksandar and Đukić, Milan and Skorić, Dejan and Mijović, Marija and Stefanović, Igor and Milivojević, Milena and Stevanović, Milena",
year = "2016",
abstract = "Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries.",
publisher = "Springer India",
journal = "Indian Pediatrics",
title = "Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia",
pages = "789-786",
number = "9",
volume = "53",
doi = "10.1007/s13312-016-0931-z"
}

Cuturilo, G., Drakulić, D., Jovanović, I., Krstić, A., Đukić, M., Skorić, D., Mijović, M., Stefanović, I., Milivojević, M.,& Stevanović, M.. (2016). Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia. in Indian Pediatrics
Springer India., 53(9), 786-789.
https://doi.org/10.1007/s13312-016-0931-z

Cuturilo G, Drakulić D, Jovanović I, Krstić A, Đukić M, Skorić D, Mijović M, Stefanović I, Milivojević M, Stevanović M. Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia. in Indian Pediatrics. 2016;53(9):786-789.
doi:10.1007/s13312-016-0931-z .

Cuturilo, Goran, Drakulić, Danijela, Jovanović, Ida, Krstić, Aleksandar, Đukić, Milan, Skorić, Dejan, Mijović, Marija, Stefanović, Igor, Milivojević, Milena, Stevanović, Milena, "Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia" in Indian Pediatrics, 53, no. 9 (2016):786-789,
https://doi.org/10.1007/s13312-016-0931-z . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Popović, Jelena
AU  - Schwirtlich, Marija
AU  - Ranković, Branislava
AU  - Stevanović, Milena
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/836
AB  - Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines
IS  - 11
VL  - 10
DO  - 10.1371/journal.pone.0143591
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Popović, Jelena and Schwirtlich, Marija and Ranković, Branislava and Stevanović, Milena",
year = "2015",
abstract = "Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines",
number = "11",
volume = "10",
doi = "10.1371/journal.pone.0143591"
}

Petrović, I., Milivojević, M., Popović, J., Schwirtlich, M., Ranković, B.,& Stevanović, M.. (2015). SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One
Public Library Science, San Francisco., 10(11).
https://doi.org/10.1371/journal.pone.0143591

Petrović I, Milivojević M, Popović J, Schwirtlich M, Ranković B, Stevanović M. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One. 2015;10(11).
doi:10.1371/journal.pone.0143591 .

Petrović, Isidora, Milivojević, Milena, Popović, Jelena, Schwirtlich, Marija, Ranković, Branislava, Stevanović, Milena, "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines" in PLoS One, 10, no. 11 (2015),
https://doi.org/10.1371/journal.pone.0143591 . .

TY  - JOUR
AU  - Jasnić, Jovana
AU  - Lazić, Andrijana
AU  - Milivojević, Milena
AU  - Mojsin, Marija
AU  - Nikčević, Gordana
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/807
AB  - Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.
PB  - Sage Publications Ltd, London
T2  - ATLA-Alternatives To Laboratory Animals
T1  - Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation
EP  - 18
IS  - 1
SP  - 9
VL  - 43
DO  - 10.1177/026119291504300105
ER  - 

@article{
author = "Jasnić, Jovana and Lazić, Andrijana and Milivojević, Milena and Mojsin, Marija and Nikčević, Gordana",
year = "2015",
abstract = "Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.",
publisher = "Sage Publications Ltd, London",
journal = "ATLA-Alternatives To Laboratory Animals",
title = "Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation",
pages = "18-9",
number = "1",
volume = "43",
doi = "10.1177/026119291504300105"
}

Jasnić, J., Lazić, A., Milivojević, M., Mojsin, M.,& Nikčević, G.. (2015). Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. in ATLA-Alternatives To Laboratory Animals
Sage Publications Ltd, London., 43(1), 9-18.
https://doi.org/10.1177/026119291504300105

Jasnić J, Lazić A, Milivojević M, Mojsin M, Nikčević G. Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. in ATLA-Alternatives To Laboratory Animals. 2015;43(1):9-18.
doi:10.1177/026119291504300105 .

Jasnić, Jovana, Lazić, Andrijana, Milivojević, Milena, Mojsin, Marija, Nikčević, Gordana, "Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation" in ATLA-Alternatives To Laboratory Animals, 43, no. 1 (2015):9-18,
https://doi.org/10.1177/026119291504300105 . .

TY  - THES
AU  - Milivojević, Milena
PY  - 2014
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=2684
UR  - https://nardus.mpn.gov.rs/handle/123456789/5076
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:10796/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024815282
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/35
AB  - Sonic Hedgehog (SHH) signalni put ima važnu ulogu u procesima koji se odvijaju tokom embrionalnog razvića u kojima kontroliše proliferaciju i diferencijaciju ćelija i učestvuje u održavanju polarnosti tkiva. Poslednjih godina mnogobrojni literaturni podaci pokazuju da promena u regulaciji SHH signalnog puta dovodi do nastanka i progresije različitih vrsta tumora kod čoveka. SHH signalni put je povezan sa meduloblastomom, leukemijom, karcinomom bazalnih ćelija, tumorima pluća, prostate, pancreasa, dojke i jajnika. Takođe, povećana ekspresija komponenti SHH signalnog puta je primećena u premalignim lezijama i ćelijama karcinoma grlića materice. SOX18 gen pripada familiji SOX gena koji kodiraju transkripcione faktore uključene u kontrolu različitih procesa tokom embrionalnog razvića. SOX18 protein ima važnu ulogu u razviću vaskularnog sistema kao i u adultnoj neovaskularizaciji. Uloga SOX18 proteina u vaskularnom razviću otkrivena je na osnovu poremećaja u razvoju vaskularnog sistema koji su uočeni kod prirodnih mutanata miša i čoveka. Osim toga, kod adultnog organizma SOX18 protein učestvuje u regulaciji angiogeneze i limfangiogeneze kako u fiziološkim stanjima organizma, tako i tokom patofizioloških promena kao što su zarastanje rana i tumorska angiogeneza. Takođe, pokazano je da se inhibicijom SOX18 funkcije utiče na limfangiogenezu čime se sprečava metastaza ćelija tumora. Najnoviji podaci pokazuju da je ekspresija SOX18 gena, detektovana u ćelijama invazivnog karcinoma dojke i tumora jajnika, a nivo ekspresije je u direktnoj korelaciji sa stadijumom tumora i može predstavljati prognostički marker. Predmet istraživanja prikazanog u ovom radu je bila analiza uloge SHH signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, in vitro model sistemu karcinoma grlića materice. Prvo je analizirana uloga GLI regulatornih proteina, finalnih efektora SHH signalne kaskade, u regulaciji transkripcije SOX18 gena. Prikazani rezultati su pokazali da su GLI1 i GLI2 pozitivni regulatori promotorske aktivnosti SOX18 gena kao i da povećavaju endogenu ekspresiju SOX18 gena, dok GLI3 transkripcioni faktor nije uticao na aktivnost SOX18 promotorskog konstrukta niti na nivo endogene ekspresije SOX18 gena u HeLa ćelijama...
AB  - The Sonic Hedgehog (SHH) signaling pathway plays important role in embrionic development directing cell proliferation and diferentiation and maintaing tissue polarity. In adults, this signaling pathway is rather suppressed. SHH signaling pathway has been associated with medulloblastoma, leukemia, basal cell carcinoma and lung, prostate, pancreatic, breast and ovarian cancers. In the last years, numerous data show that deregulation of SHH signaling pathway has been associated with onset and progression of various types of human cancer. It was shown that increased expression of HH-signaling molecules was seen in precancerous lesions and in cervical cancer. SOX18 gene is a member of SOX gene family that encodes transcription factors implicated in the control of various developmental processes. SOX18 protein plays important roles in vascular development and postnatal neovascularization. The functional importance of SOX18 protein in vascular development is revealed by the vascular defects caused by mutation in mice and humans. Also, in adults SOX18 is involved in the regulation of angiogenesis and lymphangiogenesis in physiological and pathophysiological condition such in wound healing or tumor growth. Furthermore, it has been reported that interfere with SOX18 function impairs tumor lymphangiogenesis which decreases the rate of cancer cell metastasis. In past two years, literature data show SOX18 expression in invasive ductual breast carcinoma and ovarian carcinoma and that level of expression correlates with poor prognosis suggesting that a SOX18 expression may serve as a prognostic marker. The aim of this study was to investigate the role of Sonic Hedgehog signaling pathway in the regulation of SOX18 gene expression in HeLa cells, in vitro model system of cervical cancer . We were analyzing the effect of GLI regulator proteins, final effectors of SHH signaling pathway, on transcriptional regulation of SOX18 gene expression. Presented results have shown that GLI1 and GLI2 are potent activators of SOX18 promoter activity as well as SOX18 expression in HeLa cells, while GLI3 had no effect...
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Uloga Sonic Hedgehog signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, kao model sistemu karcinoma grlića materice
T1  - The role of Sonic Hedgehog signaling pathway in the regulation of SOX18 gene expression in HeLa cells, model system of cervical carcinoma
UR  - https://hdl.handle.net/21.15107/rcub_nardus_5076
ER  - 

@phdthesis{
author = "Milivojević, Milena",
year = "2014",
abstract = "Sonic Hedgehog (SHH) signalni put ima važnu ulogu u procesima koji se odvijaju tokom embrionalnog razvića u kojima kontroliše proliferaciju i diferencijaciju ćelija i učestvuje u održavanju polarnosti tkiva. Poslednjih godina mnogobrojni literaturni podaci pokazuju da promena u regulaciji SHH signalnog puta dovodi do nastanka i progresije različitih vrsta tumora kod čoveka. SHH signalni put je povezan sa meduloblastomom, leukemijom, karcinomom bazalnih ćelija, tumorima pluća, prostate, pancreasa, dojke i jajnika. Takođe, povećana ekspresija komponenti SHH signalnog puta je primećena u premalignim lezijama i ćelijama karcinoma grlića materice. SOX18 gen pripada familiji SOX gena koji kodiraju transkripcione faktore uključene u kontrolu različitih procesa tokom embrionalnog razvića. SOX18 protein ima važnu ulogu u razviću vaskularnog sistema kao i u adultnoj neovaskularizaciji. Uloga SOX18 proteina u vaskularnom razviću otkrivena je na osnovu poremećaja u razvoju vaskularnog sistema koji su uočeni kod prirodnih mutanata miša i čoveka. Osim toga, kod adultnog organizma SOX18 protein učestvuje u regulaciji angiogeneze i limfangiogeneze kako u fiziološkim stanjima organizma, tako i tokom patofizioloških promena kao što su zarastanje rana i tumorska angiogeneza. Takođe, pokazano je da se inhibicijom SOX18 funkcije utiče na limfangiogenezu čime se sprečava metastaza ćelija tumora. Najnoviji podaci pokazuju da je ekspresija SOX18 gena, detektovana u ćelijama invazivnog karcinoma dojke i tumora jajnika, a nivo ekspresije je u direktnoj korelaciji sa stadijumom tumora i može predstavljati prognostički marker. Predmet istraživanja prikazanog u ovom radu je bila analiza uloge SHH signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, in vitro model sistemu karcinoma grlića materice. Prvo je analizirana uloga GLI regulatornih proteina, finalnih efektora SHH signalne kaskade, u regulaciji transkripcije SOX18 gena. Prikazani rezultati su pokazali da su GLI1 i GLI2 pozitivni regulatori promotorske aktivnosti SOX18 gena kao i da povećavaju endogenu ekspresiju SOX18 gena, dok GLI3 transkripcioni faktor nije uticao na aktivnost SOX18 promotorskog konstrukta niti na nivo endogene ekspresije SOX18 gena u HeLa ćelijama..., The Sonic Hedgehog (SHH) signaling pathway plays important role in embrionic development directing cell proliferation and diferentiation and maintaing tissue polarity. In adults, this signaling pathway is rather suppressed. SHH signaling pathway has been associated with medulloblastoma, leukemia, basal cell carcinoma and lung, prostate, pancreatic, breast and ovarian cancers. In the last years, numerous data show that deregulation of SHH signaling pathway has been associated with onset and progression of various types of human cancer. It was shown that increased expression of HH-signaling molecules was seen in precancerous lesions and in cervical cancer. SOX18 gene is a member of SOX gene family that encodes transcription factors implicated in the control of various developmental processes. SOX18 protein plays important roles in vascular development and postnatal neovascularization. The functional importance of SOX18 protein in vascular development is revealed by the vascular defects caused by mutation in mice and humans. Also, in adults SOX18 is involved in the regulation of angiogenesis and lymphangiogenesis in physiological and pathophysiological condition such in wound healing or tumor growth. Furthermore, it has been reported that interfere with SOX18 function impairs tumor lymphangiogenesis which decreases the rate of cancer cell metastasis. In past two years, literature data show SOX18 expression in invasive ductual breast carcinoma and ovarian carcinoma and that level of expression correlates with poor prognosis suggesting that a SOX18 expression may serve as a prognostic marker. The aim of this study was to investigate the role of Sonic Hedgehog signaling pathway in the regulation of SOX18 gene expression in HeLa cells, in vitro model system of cervical cancer . We were analyzing the effect of GLI regulator proteins, final effectors of SHH signaling pathway, on transcriptional regulation of SOX18 gene expression. Presented results have shown that GLI1 and GLI2 are potent activators of SOX18 promoter activity as well as SOX18 expression in HeLa cells, while GLI3 had no effect...",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Uloga Sonic Hedgehog signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, kao model sistemu karcinoma grlića materice, The role of Sonic Hedgehog signaling pathway in the regulation of SOX18 gene expression in HeLa cells, model system of cervical carcinoma",
url = "https://hdl.handle.net/21.15107/rcub_nardus_5076"
}

Milivojević, M.. (2014). Uloga Sonic Hedgehog signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, kao model sistemu karcinoma grlića materice. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_5076

Milivojević M. Uloga Sonic Hedgehog signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, kao model sistemu karcinoma grlića materice. 2014;.
https://hdl.handle.net/21.15107/rcub_nardus_5076 .

Milivojević, Milena, "Uloga Sonic Hedgehog signalnog puta u regulaciji ekspresije SOX18 gena u HeLa ćelijama, kao model sistemu karcinoma grlića materice" (2014),
https://hdl.handle.net/21.15107/rcub_nardus_5076 .

TY  - CONF
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Mojsin, Marija
AU  - Drakulić, Danijela
AU  - Kovačević Grujičić, Nataša
AU  - Topalović, Vladanka
AU  - Davidović, Slobodan
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/730
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line
EP  - S120
SP  - S120
VL  - 50
DO  - 10.1016/S0959-8049(14)50444-3
ER  - 

@conference{
author = "Petrović, Isidora and Milivojević, Milena and Mojsin, Marija and Drakulić, Danijela and Kovačević Grujičić, Nataša and Topalović, Vladanka and Davidović, Slobodan and Stevanović, Milena",
year = "2014",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line",
pages = "S120-S120",
volume = "50",
doi = "10.1016/S0959-8049(14)50444-3"
}

Petrović, I., Milivojević, M., Mojsin, M., Drakulić, D., Kovačević Grujičić, N., Topalović, V., Davidović, S.,& Stevanović, M.. (2014). The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 50, S120-S120.
https://doi.org/10.1016/S0959-8049(14)50444-3

Petrović I, Milivojević M, Mojsin M, Drakulić D, Kovačević Grujičić N, Topalović V, Davidović S, Stevanović M. The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line. in European Journal of Cancer. 2014;50:S120-S120.
doi:10.1016/S0959-8049(14)50444-3 .

Petrović, Isidora, Milivojević, Milena, Mojsin, Marija, Drakulić, Danijela, Kovačević Grujičić, Nataša, Topalović, Vladanka, Davidović, Slobodan, Stevanović, Milena, "The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line" in European Journal of Cancer, 50 (2014):S120-S120,
https://doi.org/10.1016/S0959-8049(14)50444-3 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Krstić, Aleksandar
AU  - Gradinac, Marija
AU  - Ilisić, Tamara
AU  - Parezanović, Vojislav
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Jovanović, Ida
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/678
AB  - Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients.
PB  - Cambridge Univ Press, Cambridge
T2  - Cardiology in the Young
T1  - The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2
EP  - 188
IS  - 2
SP  - 181
VL  - 23
DO  - 10.1017/S1047951112000571
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Krstić, Aleksandar and Gradinac, Marija and Ilisić, Tamara and Parezanović, Vojislav and Milivojević, Milena and Stevanović, Milena and Jovanović, Ida",
year = "2013",
abstract = "Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Cardiology in the Young",
title = "The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2",
pages = "188-181",
number = "2",
volume = "23",
doi = "10.1017/S1047951112000571"
}

Cuturilo, G., Drakulić, D., Krstić, A., Gradinac, M., Ilisić, T., Parezanović, V., Milivojević, M., Stevanović, M.,& Jovanović, I.. (2013). The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2. in Cardiology in the Young
Cambridge Univ Press, Cambridge., 23(2), 181-188.
https://doi.org/10.1017/S1047951112000571

Cuturilo G, Drakulić D, Krstić A, Gradinac M, Ilisić T, Parezanović V, Milivojević M, Stevanović M, Jovanović I. The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2. in Cardiology in the Young. 2013;23(2):181-188.
doi:10.1017/S1047951112000571 .

Cuturilo, Goran, Drakulić, Danijela, Krstić, Aleksandar, Gradinac, Marija, Ilisić, Tamara, Parezanović, Vojislav, Milivojević, Milena, Stevanović, Milena, Jovanović, Ida, "The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2" in Cardiology in the Young, 23, no. 2 (2013):181-188,
https://doi.org/10.1017/S1047951112000571 . .

TY  - JOUR
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Popović, Jelena
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/672
AB  - SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Biochemistry-Moscow
T1  - Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein
EP  - 1292
IS  - 11
SP  - 1287
VL  - 78
DO  - 10.1134/S0006297913110096
ER  - 

@article{
author = "Milivojević, Milena and Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Mojsin, Marija and Stevanović, Milena",
year = "2013",
abstract = "SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Biochemistry-Moscow",
title = "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein",
pages = "1292-1287",
number = "11",
volume = "78",
doi = "10.1134/S0006297913110096"
}

Milivojević, M., Petrović, I., Kovačević Grujičić, N., Popović, J., Mojsin, M.,& Stevanović, M.. (2013). Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow
Maik Nauka/Interperiodica/Springer, New York., 78(11), 1287-1292.
https://doi.org/10.1134/S0006297913110096

Milivojević M, Petrović I, Kovačević Grujičić N, Popović J, Mojsin M, Stevanović M. Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow. 2013;78(11):1287-1292.
doi:10.1134/S0006297913110096 .

Milivojević, Milena, Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Mojsin, Marija, Stevanović, Milena, "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein" in Biochemistry-Moscow, 78, no. 11 (2013):1287-1292,
https://doi.org/10.1134/S0006297913110096 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Popović, Jelena
AU  - Krstić, A.
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/526
AB  - The SOX18 transcription factor plays an important role in endothelial cell specification, angiogenesis and atherogenesis. By profiling transcription factor interactions (TranSignal TM TF Protein Array) we identified several transcription factors implicated in angiogenesis that have the ability to bind to the SOX18 optimal promoter region in vitro. In this report we focused our attention on distinct transcription factors identified by the array as belonging to AP-1 and CREB/ATF protein families. In particular, we analyzed the effects of CREB, JunB, c-Jun and ATF3 on SOX18 gene expression. Functional analysis revealed that CREB acts as a repressor, while JunB, c-Jun and ATF3 act as activators of SOX18 promoter activity. Our findings indicate that a transcriptional network that includes CREB, JunB, c-Jun and ATF3 could be involved in angiogenesis-related transcriptional regulation of the SOX18 gene.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression
EP  - 525
IS  - 3
SP  - 517
VL  - 63
DO  - 10.2298/ABS1103517P
ER  - 

@article{
author = "Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Krstić, A. and Milivojević, Milena and Stevanović, Milena",
year = "2011",
abstract = "The SOX18 transcription factor plays an important role in endothelial cell specification, angiogenesis and atherogenesis. By profiling transcription factor interactions (TranSignal TM TF Protein Array) we identified several transcription factors implicated in angiogenesis that have the ability to bind to the SOX18 optimal promoter region in vitro. In this report we focused our attention on distinct transcription factors identified by the array as belonging to AP-1 and CREB/ATF protein families. In particular, we analyzed the effects of CREB, JunB, c-Jun and ATF3 on SOX18 gene expression. Functional analysis revealed that CREB acts as a repressor, while JunB, c-Jun and ATF3 act as activators of SOX18 promoter activity. Our findings indicate that a transcriptional network that includes CREB, JunB, c-Jun and ATF3 could be involved in angiogenesis-related transcriptional regulation of the SOX18 gene.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression",
pages = "525-517",
number = "3",
volume = "63",
doi = "10.2298/ABS1103517P"
}

Petrović, I., Kovačević Grujičić, N., Popović, J., Krstić, A., Milivojević, M.,& Stevanović, M.. (2011). Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(3), 517-525.
https://doi.org/10.2298/ABS1103517P

Petrović I, Kovačević Grujičić N, Popović J, Krstić A, Milivojević M, Stevanović M. Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression. in Archives of Biological Sciences. 2011;63(3):517-525.
doi:10.2298/ABS1103517P .

Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Krstić, A., Milivojević, Milena, Stevanović, Milena, "Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression" in Archives of Biological Sciences, 63, no. 3 (2011):517-525,
https://doi.org/10.2298/ABS1103517P . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Krstić, Aleksandar
AU  - Popović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/407
AB  - To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.
PB  - Springer/Plenum Publishers, New York
T2  - Biochemical Genetics
T1  - Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution
EP  - 623
IS  - 7-8
SP  - 612
VL  - 48
DO  - 10.1007/s10528-010-9343-2
ER  - 

@article{
author = "Mojsin, Marija and Kovačević Grujičić, Nataša and Krstić, Aleksandar and Popović, Jelena and Milivojević, Milena and Stevanović, Milena",
year = "2010",
abstract = "To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Biochemical Genetics",
title = "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution",
pages = "623-612",
number = "7-8",
volume = "48",
doi = "10.1007/s10528-010-9343-2"
}

Mojsin, M., Kovačević Grujičić, N., Krstić, A., Popović, J., Milivojević, M.,& Stevanović, M.. (2010). Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics
Springer/Plenum Publishers, New York., 48(7-8), 612-623.
https://doi.org/10.1007/s10528-010-9343-2

Mojsin M, Kovačević Grujičić N, Krstić A, Popović J, Milivojević M, Stevanović M. Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics. 2010;48(7-8):612-623.
doi:10.1007/s10528-010-9343-2 .

Mojsin, Marija, Kovačević Grujičić, Nataša, Krstić, Aleksandar, Popović, Jelena, Milivojević, Milena, Stevanović, Milena, "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution" in Biochemical Genetics, 48, no. 7-8 (2010):612-623,
https://doi.org/10.1007/s10528-010-9343-2 . .