TY  - CONF
AU  - Bojić, Luka
AU  - Pejić, Jelena
AU  - Stojkovska, Jasmina
AU  - Stevanović, Milena
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Milivojević, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1262
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2366
AB  - Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.
C3  - Hemijska industrija (Chemical Industry)
T1  - Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems
EP  - 20
IS  - 1S
SP  - 20
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2366
ER  - 

@conference{
author = "Bojić, Luka and Pejić, Jelena and Stojkovska, Jasmina and Stevanović, Milena and Medić, Aleksandra and Petrović, Isidora and Milivojević, Milena",
year = "2024",
abstract = "Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems",
pages = "20-20",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2366"
}

Bojić, L., Pejić, J., Stojkovska, J., Stevanović, M., Medić, A., Petrović, I.,& Milivojević, M.. (2024). Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry), 78(1S), 20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366

Bojić L, Pejić J, Stojkovska J, Stevanović M, Medić A, Petrović I, Milivojević M. Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry). 2024;78(1S):20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

Bojić, Luka, Pejić, Jelena, Stojkovska, Jasmina, Stevanović, Milena, Medić, Aleksandra, Petrović, Isidora, Milivojević, Milena, "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):20-20,
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

TY  - CONF
AU  - Pejić, Jelena
AU  - Mojsin, Marija
AU  - Stojkovska, Jasmina
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2151
AB  - Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds
EP  - 113
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2151
ER  - 

@conference{
author = "Pejić, Jelena and Mojsin, Marija and Stojkovska, Jasmina and Medić, Aleksandra and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana and Milivojević, Milena",
year = "2023",
abstract = "Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2151"
}

Pejić, J., Mojsin, M., Stojkovska, J., Medić, A., Petrović, I., Stevanović, M., Obradović, B.,& Milivojević, M.. (2023). Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151

Pejić J, Mojsin M, Stojkovska J, Medić A, Petrović I, Stevanović M, Obradović B, Milivojević M. Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

Pejić, Jelena, Mojsin, Marija, Stojkovska, Jasmina, Medić, Aleksandra, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, Milivojević, Milena, "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):113-113,
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

TY  - CONF
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Aleksandra, Medić
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Erceg, Slaven
AU  - Stevanović, Milena
AU  - Švirtlih, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2287
AB  - Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2287
ER  - 

@conference{
author = "Lazić, Stefan and Stanisavljević Ninković, Danijela and Petrović, Isidora and Aleksandra, Medić and Milivojević, Milena and Bojić, Luka and Erceg, Slaven and Stevanović, Milena and Švirtlih, Marija",
year = "2023",
abstract = "Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2287"
}

Lazić, S., Stanisavljević Ninković, D., Petrović, I., Aleksandra, M., Milivojević, M., Bojić, L., Erceg, S., Stevanović, M.,& Švirtlih, M.. (2023). Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287

Lazić S, Stanisavljević Ninković D, Petrović I, Aleksandra M, Milivojević M, Bojić L, Erceg S, Stevanović M, Švirtlih M. Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society. 2023;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

Lazić, Stefan, Stanisavljević Ninković, Danijela, Petrović, Isidora, Aleksandra, Medić, Milivojević, Milena, Bojić, Luka, Erceg, Slaven, Stevanović, Milena, Švirtlih, Marija, "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21" in 8th Congress of the Serbian Neuroscience Society (2023):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
AU  - Petrović, Isidora
AU  - Drakulić, Danijela
AU  - Milivojević, Milena
AU  - Mojsin, Marija
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/7/6392
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1890
AB  - Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma
IS  - 7
SP  - 6392
VL  - 24
DO  - 10.3390/ijms24076392
ER  - 

@article{
author = "Stevanović, Milena and Kovačević-Grujičić, Nataša and Petrović, Isidora and Drakulić, Danijela and Milivojević, Milena and Mojsin, Marija",
year = "2023",
abstract = "Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma",
number = "7",
pages = "6392",
volume = "24",
doi = "10.3390/ijms24076392"
}

Stevanović, M., Kovačević-Grujičić, N., Petrović, I., Drakulić, D., Milivojević, M.,& Mojsin, M.. (2023). Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences, 24(7), 6392.
https://doi.org/10.3390/ijms24076392

Stevanović M, Kovačević-Grujičić N, Petrović I, Drakulić D, Milivojević M, Mojsin M. Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences. 2023;24(7):6392.
doi:10.3390/ijms24076392 .

Stevanović, Milena, Kovačević-Grujičić, Nataša, Petrović, Isidora, Drakulić, Danijela, Milivojević, Milena, Mojsin, Marija, "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma" in International Journal of Molecular Sciences, 24, no. 7 (2023):6392,
https://doi.org/10.3390/ijms24076392 . .

TY  - CONF
AU  - Krstić, Aleksandra
AU  - Pavić, Aleksandar
AU  - Balint, Vanda
AU  - Lazić, Stefan
AU  - Avdović, Edina
AU  - Marković, Zoran
AU  - Pejić, Jelena
AU  - Stevanović, Milena
AU  - Petrović, Isidora
PY  - 2022
UR  - https://doi.org/10.21175/rad.spr.abstr.book.2022.9.3
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1866
AB  - Pancreatic carcinoma represents one of the most lethal malignant diseases in the world although some
progress has been made in treating the disease in the past decades. Current multi-agent treatment options
have improved the overall survival of patients, but more effective treatment strategies are still needed. In this
paper we have characterized anticancer potential of coumarin-palladium(II) complex against pancreatic
carcinoma cells. Cells viability, colony formation and migratory potential of pancreatic carcinoma cells were
assessed in vitro, followed by evaluation of apoptosis induction and in vivo testing on zebrafish. Presented
results showed remarkable reduction in pancreatic carcinoma cells growth both in vitro and in vivo, being
effective at micromolar concentrations (0.5 M). Treatments induced apoptosis, increased BAX/BCL-2 ratio
and suppressed the expression of SOX9 and SOX18, genes shown to be significantly up-regulated in
pancreatic ductal adenocarcinoma. Importantly, treatments of the zebrafish-pancreatic adenocarcinoma
xenografts resulted in significant reduction of tumor mass, while did not provoke any adverse toxic effects
including hepatotoxicity. Presented results indicate the great potential of tested compound and the
perspective of its further development towards pancreatic cancer therapy.
C3  - RAD International concerence on radiation in various fields of research
T1  - Coumarin-palladium(II) complex acts as a potent and nontoxic anticancer agent against pancreatic carcinoma cells
IS  - Spring Edition
SP  - 34
DO  - 10.21175/rad.spr.abstr.book.2022.9.3
ER  - 

@conference{
author = "Krstić, Aleksandra and Pavić, Aleksandar and Balint, Vanda and Lazić, Stefan and Avdović, Edina and Marković, Zoran and Pejić, Jelena and Stevanović, Milena and Petrović, Isidora",
year = "2022",
abstract = "Pancreatic carcinoma represents one of the most lethal malignant diseases in the world although some
progress has been made in treating the disease in the past decades. Current multi-agent treatment options
have improved the overall survival of patients, but more effective treatment strategies are still needed. In this
paper we have characterized anticancer potential of coumarin-palladium(II) complex against pancreatic
carcinoma cells. Cells viability, colony formation and migratory potential of pancreatic carcinoma cells were
assessed in vitro, followed by evaluation of apoptosis induction and in vivo testing on zebrafish. Presented
results showed remarkable reduction in pancreatic carcinoma cells growth both in vitro and in vivo, being
effective at micromolar concentrations (0.5 M). Treatments induced apoptosis, increased BAX/BCL-2 ratio
and suppressed the expression of SOX9 and SOX18, genes shown to be significantly up-regulated in
pancreatic ductal adenocarcinoma. Importantly, treatments of the zebrafish-pancreatic adenocarcinoma
xenografts resulted in significant reduction of tumor mass, while did not provoke any adverse toxic effects
including hepatotoxicity. Presented results indicate the great potential of tested compound and the
perspective of its further development towards pancreatic cancer therapy.",
journal = "RAD International concerence on radiation in various fields of research",
title = "Coumarin-palladium(II) complex acts as a potent and nontoxic anticancer agent against pancreatic carcinoma cells",
number = "Spring Edition",
pages = "34",
doi = "10.21175/rad.spr.abstr.book.2022.9.3"
}

Krstić, A., Pavić, A., Balint, V., Lazić, S., Avdović, E., Marković, Z., Pejić, J., Stevanović, M.,& Petrović, I.. (2022). Coumarin-palladium(II) complex acts as a potent and nontoxic anticancer agent against pancreatic carcinoma cells. in RAD International concerence on radiation in various fields of research(Spring Edition), 34.
https://doi.org/10.21175/rad.spr.abstr.book.2022.9.3

Krstić A, Pavić A, Balint V, Lazić S, Avdović E, Marković Z, Pejić J, Stevanović M, Petrović I. Coumarin-palladium(II) complex acts as a potent and nontoxic anticancer agent against pancreatic carcinoma cells. in RAD International concerence on radiation in various fields of research. 2022;(Spring Edition):34.
doi:10.21175/rad.spr.abstr.book.2022.9.3 .

Krstić, Aleksandra, Pavić, Aleksandar, Balint, Vanda, Lazić, Stefan, Avdović, Edina, Marković, Zoran, Pejić, Jelena, Stevanović, Milena, Petrović, Isidora, "Coumarin-palladium(II) complex acts as a potent and nontoxic anticancer agent against pancreatic carcinoma cells" in RAD International concerence on radiation in various fields of research, no. Spring Edition (2022):34,
https://doi.org/10.21175/rad.spr.abstr.book.2022.9.3 . .

TY  - JOUR
AU  - Krstić, Aleksandra
AU  - Pavić, Aleksandar
AU  - Avdović, Edina H.
AU  - Marković, Zoran
AU  - Stevanović, Milena
AU  - Petrović, Isidora
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1559
AB  - Pancreatic carcinoma still represents one of the most lethal malignant diseases in the world although some progress has been made in treating the disease in the past decades. Current multi-agent treatment options have improved the overall survival of patients, however, more effective treatment strategies are still needed. In this paper we have characterized the anticancer potential of coumarin-palladium(II) complex against pancreatic carcinoma cells. Cells viability, colony formation and migratory potential of pancreatic carcinoma cells were assessed in vitro, followed by evaluation of apoptosis induction and in vivo testing on zebrafish. Presented results showed remarkable reduction in pancreatic carcinoma cells growth both in vitro and in vivo, being effective at micromolar concentrations (0.5 mu M). Treatments induced apoptosis, increased BAX/BCL-2 ratio and suppressed the expression of SOX9 and SOX18, genes shown to be significantly up-regulated in pancreatic ductal adenocarcinoma. Importantly, treatments of the zebrafish-pancreatic adenocarcinoma xenografts resulted in significant reduction in tumor mass, without provoking any adverse toxic effects including hepatotoxicity. Presented results indicate the great potential of the tested compound and the perspective of its further development towards pancreatic cancer therapy.
PB  - MDPI, Basel
T2  - Molecules
T1  - Coumarin-Palladium(II) Complex Acts as a Potent and Non-Toxic Anticancer Agent against Pancreatic Carcinoma Cells
IS  - 7
VL  - 27
DO  - 10.3390/molecules27072115
ER  - 

@article{
author = "Krstić, Aleksandra and Pavić, Aleksandar and Avdović, Edina H. and Marković, Zoran and Stevanović, Milena and Petrović, Isidora",
year = "2022",
abstract = "Pancreatic carcinoma still represents one of the most lethal malignant diseases in the world although some progress has been made in treating the disease in the past decades. Current multi-agent treatment options have improved the overall survival of patients, however, more effective treatment strategies are still needed. In this paper we have characterized the anticancer potential of coumarin-palladium(II) complex against pancreatic carcinoma cells. Cells viability, colony formation and migratory potential of pancreatic carcinoma cells were assessed in vitro, followed by evaluation of apoptosis induction and in vivo testing on zebrafish. Presented results showed remarkable reduction in pancreatic carcinoma cells growth both in vitro and in vivo, being effective at micromolar concentrations (0.5 mu M). Treatments induced apoptosis, increased BAX/BCL-2 ratio and suppressed the expression of SOX9 and SOX18, genes shown to be significantly up-regulated in pancreatic ductal adenocarcinoma. Importantly, treatments of the zebrafish-pancreatic adenocarcinoma xenografts resulted in significant reduction in tumor mass, without provoking any adverse toxic effects including hepatotoxicity. Presented results indicate the great potential of the tested compound and the perspective of its further development towards pancreatic cancer therapy.",
publisher = "MDPI, Basel",
journal = "Molecules",
title = "Coumarin-Palladium(II) Complex Acts as a Potent and Non-Toxic Anticancer Agent against Pancreatic Carcinoma Cells",
number = "7",
volume = "27",
doi = "10.3390/molecules27072115"
}

Krstić, A., Pavić, A., Avdović, E. H., Marković, Z., Stevanović, M.,& Petrović, I.. (2022). Coumarin-Palladium(II) Complex Acts as a Potent and Non-Toxic Anticancer Agent against Pancreatic Carcinoma Cells. in Molecules
MDPI, Basel., 27(7).
https://doi.org/10.3390/molecules27072115

Krstić A, Pavić A, Avdović EH, Marković Z, Stevanović M, Petrović I. Coumarin-Palladium(II) Complex Acts as a Potent and Non-Toxic Anticancer Agent against Pancreatic Carcinoma Cells. in Molecules. 2022;27(7).
doi:10.3390/molecules27072115 .

Krstić, Aleksandra, Pavić, Aleksandar, Avdović, Edina H., Marković, Zoran, Stevanović, Milena, Petrović, Isidora, "Coumarin-Palladium(II) Complex Acts as a Potent and Non-Toxic Anticancer Agent against Pancreatic Carcinoma Cells" in Molecules, 27, no. 7 (2022),
https://doi.org/10.3390/molecules27072115 . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Petrović, Isidora
AU  - Mojsin, Marija
AU  - Milivojević, Milena
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1519
AB  - Glioblastoma (GBM) is the most common and highly lethal type of brain tumor, with poor survival despite advances in understanding its complexity. After current standard therapeutic treatment, including tumor resection, radiotherapy and concomitant chemotherapy with temozolomide, the median overall survival of patients with this type of tumor is less than 15 months. Thus, there is an urgent need for new insights into GBM molecular characteristics and progress in targeted therapy in order to improve clinical outcomes. The literature data revealed that a number of different signaling pathways are dysregulated in GBM. In this review, we intended to summarize and discuss current literature data and therapeutic modalities focused on targeting dysregulated signaling pathways in GBM. A better understanding of opportunities for targeting signaling pathways that influences malignant behavior of GBM cells might open the way for the development of novel GBM-targeted therapies.
PB  - MDPI, Basel
T2  - Cells
T1  - Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma
IS  - 16
VL  - 11
DO  - 10.3390/cells11162530
ER  - 

@article{
author = "Drakulić, Danijela and Schwirtlich, Marija and Petrović, Isidora and Mojsin, Marija and Milivojević, Milena and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2022",
abstract = "Glioblastoma (GBM) is the most common and highly lethal type of brain tumor, with poor survival despite advances in understanding its complexity. After current standard therapeutic treatment, including tumor resection, radiotherapy and concomitant chemotherapy with temozolomide, the median overall survival of patients with this type of tumor is less than 15 months. Thus, there is an urgent need for new insights into GBM molecular characteristics and progress in targeted therapy in order to improve clinical outcomes. The literature data revealed that a number of different signaling pathways are dysregulated in GBM. In this review, we intended to summarize and discuss current literature data and therapeutic modalities focused on targeting dysregulated signaling pathways in GBM. A better understanding of opportunities for targeting signaling pathways that influences malignant behavior of GBM cells might open the way for the development of novel GBM-targeted therapies.",
publisher = "MDPI, Basel",
journal = "Cells",
title = "Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma",
number = "16",
volume = "11",
doi = "10.3390/cells11162530"
}

Drakulić, D., Schwirtlich, M., Petrović, I., Mojsin, M., Milivojević, M., Kovačević Grujičić, N.,& Stevanović, M.. (2022). Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma. in Cells
MDPI, Basel., 11(16).
https://doi.org/10.3390/cells11162530

Drakulić D, Schwirtlich M, Petrović I, Mojsin M, Milivojević M, Kovačević Grujičić N, Stevanović M. Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma. in Cells. 2022;11(16).
doi:10.3390/cells11162530 .

Drakulić, Danijela, Schwirtlich, Marija, Petrović, Isidora, Mojsin, Marija, Milivojević, Milena, Kovačević Grujičić, Nataša, Stevanović, Milena, "Current Opportunities for Targeting Dysregulated Neurodevelopmental Signaling Pathways in Glioblastoma" in Cells, 11, no. 16 (2022),
https://doi.org/10.3390/cells11162530 . .

TY  - CONF
AU  - Lazić, Stefan
AU  - Dužanić, Filip
AU  - Stanisavljević Ninković, Danijela
AU  - Drakulić, Danijela
AU  - Mojsin, Marija
AU  - Milojević, Milena
AU  - Balinat, Vanda
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša 
AU  - Schwirtlich, Marija
AU  - Stevanović, Milena
PY  - 2022
UR  - https://doi.org/10.21175/rad.spr.abstr.book.2022.22.2
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1865
AB  - The family of SOX genes encodes proteins that display properties of both classical transcription factors and architectural components of chromatin. During development of nervous system, as well as adult neurogenesis, SOX transcription factors govern diverse cellular processes such as maintaining the multipotency of neural stem cells, cell proliferation, cell fate decision, migration as well as terminal differentiation of neurons. Despite their well-known function in development and brain homeostasis, the expression and role of these genes in pathology- induced neural stem cell plasticity is poorly understood. Reduction in oxygen supply or ischemia are involved in various pathological conditions, such as stroke, traumatic brain injury and cardiac arrest, which promotes neurogenesis, angiogenesis, cell proliferation and other cell mechanisms for survival under the stress. The aim of the present study was to analyze the expression of SOX genes during in vitro neurogenesis following chemical hypoxia. Neuronal differentiation of human pluripotent embryonal carcinoma stem cell line NT2/D1 was used as an in vitro model system for studying the process of human neurogenesis. Depending on different concentration, RA directed the differentiation of NT2/D1 cells into neurons with a different phenotype. The effect of stress caused by hypoxia on the properties of pluripotent cells as well as the induction of neural differentiation was monitored in vitro by culturing NT2/D1 cells in the presence of cobalt chloride, a chemical inducer of hypoxia. The results of the analysis showed that the effect of hypoxia on the expression of SOX2 and OCT4 proteins involved in maintaining the pluripotency of cells depends on the duration of action of cobalt chloride. After short-term exposure of the cells, an increase in the levels of expression of SOX2 and OCT4 proteins was detected, while long-term treatment of the cells led to a decrease in the expression of these proteins. Furthermore, results showed that depending of duration of cobalt chloride treatments, the level of expression of miR-21 in undifferentiated NT2/D1 cells significantly changed. In addition, long-term pretreatment of pluripotent cells with cobalt chloride resulted in increased expression levels of SOX2, SOX3 and GAD67 proteins in neural progenitors induced for 7 days in the presence of, either low or high concentration of retinoic acid, indicating that hypoxia causes increased efficiency of NT2/D1 cell neural differentiation. Damage of brain tissue caused by reduction of oxygen and/or blood flow to the tissue is the leading cause of death worldwide and the leading cause of disability in humans. Our results contributes to the research focused on discovering the roles of SOX TFs and their gene targets in ischemia related pathologies, making them promising biomarkers and potential targets for future diagnostic and therapeutic strategies.
C3  - RAD International concerence on radiation in various fields of research
T1  - Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells
IS  - Spring Edition
SP  - 91
DO  - 10.21175/rad.spr.abstr.book.2022.22.2
ER  - 

@conference{
author = "Lazić, Stefan and Dužanić, Filip and Stanisavljević Ninković, Danijela and Drakulić, Danijela and Mojsin, Marija and Milojević, Milena and Balinat, Vanda and Petrović, Isidora and Kovačević Grujičić, Nataša  and Schwirtlich, Marija and Stevanović, Milena",
year = "2022",
abstract = "The family of SOX genes encodes proteins that display properties of both classical transcription factors and architectural components of chromatin. During development of nervous system, as well as adult neurogenesis, SOX transcription factors govern diverse cellular processes such as maintaining the multipotency of neural stem cells, cell proliferation, cell fate decision, migration as well as terminal differentiation of neurons. Despite their well-known function in development and brain homeostasis, the expression and role of these genes in pathology- induced neural stem cell plasticity is poorly understood. Reduction in oxygen supply or ischemia are involved in various pathological conditions, such as stroke, traumatic brain injury and cardiac arrest, which promotes neurogenesis, angiogenesis, cell proliferation and other cell mechanisms for survival under the stress. The aim of the present study was to analyze the expression of SOX genes during in vitro neurogenesis following chemical hypoxia. Neuronal differentiation of human pluripotent embryonal carcinoma stem cell line NT2/D1 was used as an in vitro model system for studying the process of human neurogenesis. Depending on different concentration, RA directed the differentiation of NT2/D1 cells into neurons with a different phenotype. The effect of stress caused by hypoxia on the properties of pluripotent cells as well as the induction of neural differentiation was monitored in vitro by culturing NT2/D1 cells in the presence of cobalt chloride, a chemical inducer of hypoxia. The results of the analysis showed that the effect of hypoxia on the expression of SOX2 and OCT4 proteins involved in maintaining the pluripotency of cells depends on the duration of action of cobalt chloride. After short-term exposure of the cells, an increase in the levels of expression of SOX2 and OCT4 proteins was detected, while long-term treatment of the cells led to a decrease in the expression of these proteins. Furthermore, results showed that depending of duration of cobalt chloride treatments, the level of expression of miR-21 in undifferentiated NT2/D1 cells significantly changed. In addition, long-term pretreatment of pluripotent cells with cobalt chloride resulted in increased expression levels of SOX2, SOX3 and GAD67 proteins in neural progenitors induced for 7 days in the presence of, either low or high concentration of retinoic acid, indicating that hypoxia causes increased efficiency of NT2/D1 cell neural differentiation. Damage of brain tissue caused by reduction of oxygen and/or blood flow to the tissue is the leading cause of death worldwide and the leading cause of disability in humans. Our results contributes to the research focused on discovering the roles of SOX TFs and their gene targets in ischemia related pathologies, making them promising biomarkers and potential targets for future diagnostic and therapeutic strategies.",
journal = "RAD International concerence on radiation in various fields of research",
title = "Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells",
number = "Spring Edition",
pages = "91",
doi = "10.21175/rad.spr.abstr.book.2022.22.2"
}

Lazić, S., Dužanić, F., Stanisavljević Ninković, D., Drakulić, D., Mojsin, M., Milojević, M., Balinat, V., Petrović, I., Kovačević Grujičić, N., Schwirtlich, M.,& Stevanović, M.. (2022). Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells. in RAD International concerence on radiation in various fields of research(Spring Edition), 91.
https://doi.org/10.21175/rad.spr.abstr.book.2022.22.2

Lazić S, Dužanić F, Stanisavljević Ninković D, Drakulić D, Mojsin M, Milojević M, Balinat V, Petrović I, Kovačević Grujičić N, Schwirtlich M, Stevanović M. Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells. in RAD International concerence on radiation in various fields of research. 2022;(Spring Edition):91.
doi:10.21175/rad.spr.abstr.book.2022.22.2 .

Lazić, Stefan, Dužanić, Filip, Stanisavljević Ninković, Danijela, Drakulić, Danijela, Mojsin, Marija, Milojević, Milena, Balinat, Vanda, Petrović, Isidora, Kovačević Grujičić, Nataša , Schwirtlich, Marija, Stevanović, Milena, "Hypoxia affects the expression of SOX genes and induction of neural differentiation of human embryonal carcinoma NT2/D1 cells" in RAD International concerence on radiation in various fields of research, no. Spring Edition (2022):91,
https://doi.org/10.21175/rad.spr.abstr.book.2022.22.2 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Arsenijević, Ana
AU  - Lazić, Andrijana
AU  - Kovačević Grujičić, Nataša
AU  - Schwirtlich, Marija
AU  - Popović, Jelena
AU  - Stevanović, Milena
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1505
AB  - Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.
PB  - Tech Science Press, Henderson
T2  - Biocell
T1  - Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells
EP  - 1367
IS  - 5
SP  - 1355
VL  - 45
DO  - 10.32604/biocell.2021.015817
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Arsenijević, Ana and Lazić, Andrijana and Kovačević Grujičić, Nataša and Schwirtlich, Marija and Popović, Jelena and Stevanović, Milena",
year = "2021",
abstract = "Genetic and molecular heterogeneity, together with intrinsic and acquired resistance to therapy, represent the major obstacles to the successful treatment of different types of breast carcinoma. Increasing evidence demonstrates that SOX transcription factors in breast carcinomas could act both as oncogenes and tumor suppressors and have been associated with tumor stage and grade, poor prognosis, and therapy resistance. Both SOX2 and SOX18 overexpression has been correlated with poor prognosis in breast carcinomas, and these genes are recognized as potential antitumor targets. Our aim was to evaluate the effect of retinoic acid (RA), a well-known cyto-differentiating agent, on breast carcinoma cells in vitro and to investigate the potential of RA treatment to modify the expression of SOX2 and SOX18 genes. By applying various experimental approaches, we evaluated the effect of RA on basic cellular processes in SK-BR-3 and MCF7 breast carcinoma cell lines. We have shown that RA inhibits cell growth, reduces the number of Ki-67 positive cells, and causes cell-cycle arrest. RA effect was more prominent in SK-BR-3 cell line that lacks SOX2 expression, including a higher decrease in cell viability, reduction in colony formation, and significant remodeling of cellular structure. We have shown that RA treatment led to the downregulation of SOX2 expression in MCF7 cells and to the reduction of SOX18 expression in both cell lines. By functional analysis, we showed that the anti-proliferative effect of RA in both cell lines was not based on the activity of stemness marker SOX2, pointing to a SOX2-independent mechanism of action. The ability of RA to reduce SOX2/SOX18 expression raises the possibility that these genes can be used as biomarkers to distinguish RA-responders from non-responders. Together, our study shows that the response of breast carcinoma cell lines to RA treatment may vary, highlighting that the development of RA-based therapy should consider differences in breast carcinoma subtypes.",
publisher = "Tech Science Press, Henderson",
journal = "Biocell",
title = "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells",
pages = "1367-1355",
number = "5",
volume = "45",
doi = "10.32604/biocell.2021.015817"
}

Petrović, I., Milivojević, M., Arsenijević, A., Lazić, A., Kovačević Grujičić, N., Schwirtlich, M., Popović, J.,& Stevanović, M.. (2021). Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell
Tech Science Press, Henderson., 45(5), 1355-1367.
https://doi.org/10.32604/biocell.2021.015817

Petrović I, Milivojević M, Arsenijević A, Lazić A, Kovačević Grujičić N, Schwirtlich M, Popović J, Stevanović M. Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells. in Biocell. 2021;45(5):1355-1367.
doi:10.32604/biocell.2021.015817 .

Petrović, Isidora, Milivojević, Milena, Arsenijević, Ana, Lazić, Andrijana, Kovačević Grujičić, Nataša, Schwirtlich, Marija, Popović, Jelena, Stevanović, Milena, "Retinoic acid affects basic cellular processes and SOX2 and SOX18 expression in breast carcinoma cells" in Biocell, 45, no. 5 (2021):1355-1367,
https://doi.org/10.32604/biocell.2021.015817 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Saso, Luciano
AU  - Dimitrić Marković, Jasmina M.
AU  - Filipović, Nenad D.
AU  - Zivić, Miroslav Z.
AU  - Cvetić Antić, Tijana N.
AU  - Zizić, Milan V.
AU  - Todorović, Nataša V.
AU  - Vukić, Milena
AU  - Trifunović, Srecko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1443
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora and Stevanović, Milena and Saso, Luciano and Dimitrić Marković, Jasmina M. and Filipović, Nenad D. and Zivić, Miroslav Z. and Cvetić Antić, Tijana N. and Zizić, Milan V. and Todorović, Nataša V. and Vukić, Milena and Trifunović, Srecko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568"
}

Avdović, E. H., Petrović, I., Stevanović, M., Saso, L., Dimitrić Marković, J. M., Filipović, N. D., Zivić, M. Z., Cvetić Antić, T. N., Zizić, M. V., Todorović, N. V., Vukić, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2021.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović I, Stevanović M, Saso L, Dimitrić Marković JM, Filipović ND, Zivić MZ, Cvetić Antić TN, Zizić MV, Todorović NV, Vukić M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora, Stevanović, Milena, Saso, Luciano, Dimitrić Marković, Jasmina M., Filipović, Nenad D., Zivić, Miroslav Z., Cvetić Antić, Tijana N., Zizić, Milan V., Todorović, Nataša V., Vukić, Milena, Trifunović, Srecko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Zvicer, Jovana
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1363
AB  - Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska Industrija
T1  - Validation of a novel perfusion bioreactor system in cancer research
EP  - 196
IS  - 3
SP  - 187
VL  - 74
DO  - 10.2298/HEMIND200329015S
ER  - 

@article{
author = "Stojkovska, Jasmina and Zvicer, Jovana and Milivojević, Milena and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana",
year = "2020",
abstract = "Development of drugs is a complex, time- and cost-consuming process due to the lack of standardized and reliable characterization techniques and models. Traditionally, drug screening is based on in vitro analysis using two-dimensional (2D) cell cultures followed by in vivo animal testing. Unfortunately, application of the obtained results to humans in about 90 % of cases fails. Therefore, it is important to develop and improve cell-based systems that can mimic the in vivo-like conditions to provide more reliable results. In this paper, we present development and validation of a novel, user-friendly perfusion bioreactor system for single use aimed for cancer research, drug screening, anti-cancer drug response studies, biomaterial characterization, and tissue engineering. Simple design of the perfusion bioreactor provides direct medium flow at physiological velocities (100-250 mu m s(-1)) through samples of different sizes and shapes. Biocompatibility of the bioreactor was confirmed in short term cultivation studies of cervical carcinoma SiHa cells immobilized in alginate microfibers under continuous medium flow. The results have shown preserved cell viability indicating that the perfusion bioreactor in conjunction with alginate hydrogels as cell carriers could be potentially used as a tool for controlled anti-cancer drug screening in a 3D environment.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska Industrija",
title = "Validation of a novel perfusion bioreactor system in cancer research",
pages = "196-187",
number = "3",
volume = "74",
doi = "10.2298/HEMIND200329015S"
}

Stojkovska, J., Zvicer, J., Milivojević, M., Petrović, I., Stevanović, M.,& Obradović, B.. (2020). Validation of a novel perfusion bioreactor system in cancer research. in Hemijska Industrija
Savez hemijskih inženjera, Beograd., 74(3), 187-196.
https://doi.org/10.2298/HEMIND200329015S

Stojkovska J, Zvicer J, Milivojević M, Petrović I, Stevanović M, Obradović B. Validation of a novel perfusion bioreactor system in cancer research. in Hemijska Industrija. 2020;74(3):187-196.
doi:10.2298/HEMIND200329015S .

Stojkovska, Jasmina, Zvicer, Jovana, Milivojević, Milena, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, "Validation of a novel perfusion bioreactor system in cancer research" in Hemijska Industrija, 74, no. 3 (2020):187-196,
https://doi.org/10.2298/HEMIND200329015S . .

TY  - JOUR
AU  - Dimić, Dusan S.
AU  - Marković, Zoran S.
AU  - Saso, Luciano
AU  - Avdović, Edina H.
AU  - Dorović, Jelena R.
AU  - Petrović, Isidora
AU  - Stanisavljević Ninković, Danijela
AU  - Stevanović, Milena
AU  - Potocnak, Ivan
AU  - Samol'ova, Erika
AU  - Trifunović, Srecko R.
AU  - Marković, Jasmina M. Dimitric
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1295
AB  - The newly synthesized coumarin derivative with dopamine, 3-(1-((3,4-dihydroxyphenethyDamino)ethylidene)-chroman-2,4-dione, was completely structurally characterized by X-ray crystallography. It was shown that several types of hydrogen bonds are present, which additionally stabilize the structure. The compound was tested in vitro against different cell lines, healthy human keratinocyte HaCaT, cervical squamous cell carcinoma SiHa, breast carcinoma MCF7, and hepatocellular carcinoma HepG2. Compared to control, the new derivate showed a stronger effect on both healthy and carcinoma cell lines, with the most prominent effect on the breast carcinoma MCF7 cell line. The molecular docking study, obtained for ten different conformations of the new compound, showed its inhibitory nature against CDKS protein. Lower inhibition constant, relative to one of 4-OH-coumarine, proved stronger and more numerous interactions with CDKS protein. These interactions were carefully examined for both parent molecule and derivative and explained from a structural point of view.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent
VL  - 2019
DO  - 10.1155/2019/2069250
ER  - 

@article{
author = "Dimić, Dusan S. and Marković, Zoran S. and Saso, Luciano and Avdović, Edina H. and Dorović, Jelena R. and Petrović, Isidora and Stanisavljević Ninković, Danijela and Stevanović, Milena and Potocnak, Ivan and Samol'ova, Erika and Trifunović, Srecko R. and Marković, Jasmina M. Dimitric",
year = "2019",
abstract = "The newly synthesized coumarin derivative with dopamine, 3-(1-((3,4-dihydroxyphenethyDamino)ethylidene)-chroman-2,4-dione, was completely structurally characterized by X-ray crystallography. It was shown that several types of hydrogen bonds are present, which additionally stabilize the structure. The compound was tested in vitro against different cell lines, healthy human keratinocyte HaCaT, cervical squamous cell carcinoma SiHa, breast carcinoma MCF7, and hepatocellular carcinoma HepG2. Compared to control, the new derivate showed a stronger effect on both healthy and carcinoma cell lines, with the most prominent effect on the breast carcinoma MCF7 cell line. The molecular docking study, obtained for ten different conformations of the new compound, showed its inhibitory nature against CDKS protein. Lower inhibition constant, relative to one of 4-OH-coumarine, proved stronger and more numerous interactions with CDKS protein. These interactions were carefully examined for both parent molecule and derivative and explained from a structural point of view.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent",
volume = "2019",
doi = "10.1155/2019/2069250"
}

Dimić, D. S., Marković, Z. S., Saso, L., Avdović, E. H., Dorović, J. R., Petrović, I., Stanisavljević Ninković, D., Stevanović, M., Potocnak, I., Samol'ova, E., Trifunović, S. R.,& Marković, J. M. D.. (2019). Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2019.
https://doi.org/10.1155/2019/2069250

Dimić DS, Marković ZS, Saso L, Avdović EH, Dorović JR, Petrović I, Stanisavljević Ninković D, Stevanović M, Potocnak I, Samol'ova E, Trifunović SR, Marković JMD. Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/2069250 .

Dimić, Dusan S., Marković, Zoran S., Saso, Luciano, Avdović, Edina H., Dorović, Jelena R., Petrović, Isidora, Stanisavljević Ninković, Danijela, Stevanović, Milena, Potocnak, Ivan, Samol'ova, Erika, Trifunović, Srecko R., Marković, Jasmina M. Dimitric, "Synthesis and Characterization of 3-(1-((3,4-Dihydroxyphenethyl)amino)ethylidene)-chroman-2,4-dione as a Potential Antitumor Agent" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/2069250 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1629
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Davidović, Slobodan
AU  - Atkinson, Michael J.
AU  - Anastasov, Nataša
AU  - Stevanović, Milena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1206
AB  - Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.
PB  - Taylor & Francis Ltd, Abingdon
T2  - International Journal of Radiation Biology
T1  - Radiation effects on early phase of NT2/D1 neural differentiation in vitro
EP  - 1639
IS  - 12
SP  - 1627
VL  - 95
DO  - 10.1080/09553002.2019.1665207
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Popović, Jelena and Petrović, Isidora and Davidović, Slobodan and Atkinson, Michael J. and Anastasov, Nataša and Stevanović, Milena",
year = "2019",
abstract = "Purpose: Widespread medical use of radiation in diagnosis, imaging and treatment of different central nervous system malignancies lead to various consequences. Aim of this study was to further elucidate mechanism of cell response to radiation and possible consequence on neural differentiation. Materials and methods: NT2/D1 cells that resemble neural progenitors were used as a model system. Undifferentiated NT2/D1 cells and NT2/D1 cells in the early phase of neural differentiation were irradiated with low (0.2 Gy) and moderate (2 Gy) doses of gamma radiation. The effect was analyzed on apoptosis, cell cycle, senescence, spheroid formation and the expression of genes and miRNAs involved in the regulation of pluripotency or neural differentiation. Results: Two grays of irradiation induced apoptosis, senescence and cell cycle arrest of NT2/D1 cells, accompanied with altered expression of several genes (SOX2, OCT4, SOX3, PAX6) and miRNAs (miR-219, miR-21, miR124-a). Presented results show that 2 Gy of radiation significantly affected early phase of neural differentiation in vitro. Conclusions: These results suggest that 2 Gy of radiation significantly affected early phase of neural differentiation and affect the population of neural progenitors. These findings might help in better understanding of side effects of radiotherapy in treatments of central nervous system malignancies.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "International Journal of Radiation Biology",
title = "Radiation effects on early phase of NT2/D1 neural differentiation in vitro",
pages = "1639-1627",
number = "12",
volume = "95",
doi = "10.1080/09553002.2019.1665207"
}

Stanisavljević Ninković, D., Popović, J., Petrović, I., Davidović, S., Atkinson, M. J., Anastasov, N.,& Stevanović, M.. (2019). Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology
Taylor & Francis Ltd, Abingdon., 95(12), 1627-1639.
https://doi.org/10.1080/09553002.2019.1665207

Stanisavljević Ninković D, Popović J, Petrović I, Davidović S, Atkinson MJ, Anastasov N, Stevanović M. Radiation effects on early phase of NT2/D1 neural differentiation in vitro. in International Journal of Radiation Biology. 2019;95(12):1627-1639.
doi:10.1080/09553002.2019.1665207 .

Stanisavljević Ninković, Danijela, Popović, Jelena, Petrović, Isidora, Davidović, Slobodan, Atkinson, Michael J., Anastasov, Nataša, Stevanović, Milena, "Radiation effects on early phase of NT2/D1 neural differentiation in vitro" in International Journal of Radiation Biology, 95, no. 12 (2019):1627-1639,
https://doi.org/10.1080/09553002.2019.1665207 . .

TY  - JOUR
AU  - Tošić, Nataša
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Davidović, Slobodan
AU  - Virijević, Marijana
AU  - Suvajdžić-Vuković, Nada
AU  - Pavlović, Sonja
AU  - Stevanović, Milena
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1196
AB  - Aberrant expression of different SOX (SRY-related high mobility group (HMG) box) genes has been observed in number of tumors but, little is known about their expression patterns in hematological malignancies, especially in acute myeloid leukemia (AML). In this study we investigated SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in 50 de novo adult AML patients and correlated our findings with known clinical and molecular prognostic markers of the disease. We have found that these genes are overexpressed in 10-22% of patients and preliminary findings suggest that high expression level of these genes may have prognostic significance in AML patients. This is the first study focused on examining the expression level of SOX2, SOX3, SOX11, SOX14 and SOX18 genes in AML patients. Although this is a relatively limited study, initial findings indicate the need for further investigation of these genes, their potential roles in leukemia pathogenesis as well as prognosis in AML patients.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Leukemia Research
T1  - Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia
EP  - 38
SP  - 32
VL  - 67
DO  - 10.1016/j.leukres.2018.02.001
ER  - 

@article{
author = "Tošić, Nataša and Petrović, Isidora and Kovačević Grujičić, Nataša and Davidović, Slobodan and Virijević, Marijana and Suvajdžić-Vuković, Nada and Pavlović, Sonja and Stevanović, Milena",
year = "2018",
abstract = "Aberrant expression of different SOX (SRY-related high mobility group (HMG) box) genes has been observed in number of tumors but, little is known about their expression patterns in hematological malignancies, especially in acute myeloid leukemia (AML). In this study we investigated SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in 50 de novo adult AML patients and correlated our findings with known clinical and molecular prognostic markers of the disease. We have found that these genes are overexpressed in 10-22% of patients and preliminary findings suggest that high expression level of these genes may have prognostic significance in AML patients. This is the first study focused on examining the expression level of SOX2, SOX3, SOX11, SOX14 and SOX18 genes in AML patients. Although this is a relatively limited study, initial findings indicate the need for further investigation of these genes, their potential roles in leukemia pathogenesis as well as prognosis in AML patients.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Leukemia Research",
title = "Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia",
pages = "38-32",
volume = "67",
doi = "10.1016/j.leukres.2018.02.001"
}

Tošić, N., Petrović, I., Kovačević Grujičić, N., Davidović, S., Virijević, M., Suvajdžić-Vuković, N., Pavlović, S.,& Stevanović, M.. (2018). Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia. in Leukemia Research
Pergamon-Elsevier Science Ltd, Oxford., 67, 32-38.
https://doi.org/10.1016/j.leukres.2018.02.001

Tošić N, Petrović I, Kovačević Grujičić N, Davidović S, Virijević M, Suvajdžić-Vuković N, Pavlović S, Stevanović M. Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia. in Leukemia Research. 2018;67:32-38.
doi:10.1016/j.leukres.2018.02.001 .

Tošić, Nataša, Petrović, Isidora, Kovačević Grujičić, Nataša, Davidović, Slobodan, Virijević, Marijana, Suvajdžić-Vuković, Nada, Pavlović, Sonja, Stevanović, Milena, "Prognostic significance of SOX2, SOX3, SOX11, SOX14 and SOX18 gene expression in adult de novo acute myeloid leukemia" in Leukemia Research, 67 (2018):32-38,
https://doi.org/10.1016/j.leukres.2018.02.001 . .

TY  - JOUR
AU  - Stojković, Dejan S.
AU  - Kovačević Grujičić, Nataša
AU  - Reis, Filipa S.
AU  - Davidović, Slobodan
AU  - Barros, Lillian
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Pavić, Aleksandar
AU  - Glamoclija, Jasmina
AU  - Cirić, Ana
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C. F. R.
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1051
AB  - Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.
PB  - Elsevier Science Bv, Amsterdam
T2  - Lwt-Food Science and Technology
T1  - Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract
EP  - 462
SP  - 454
VL  - 79
DO  - 10.1016/j.lwt.2017.01.045
ER  - 

@article{
author = "Stojković, Dejan S. and Kovačević Grujičić, Nataša and Reis, Filipa S. and Davidović, Slobodan and Barros, Lillian and Popović, Jelena and Petrović, Isidora and Pavić, Aleksandar and Glamoclija, Jasmina and Cirić, Ana and Stevanović, Milena and Ferreira, Isabel C. F. R. and Soković, Marina",
year = "2017",
abstract = "Wild Meripilus giganteus Karst belongs to the order Polyporales, in which some members are known to possess a wide range of pharmacological properties. M. giganteus showed to be rich in carbohydrates (74.49 g/100 g) and proteins (15.94 g/100 g), presenting low fat content (1.51 g/100 g). Chemical composition was determined by using chromatographic techniques. Also, various bioactive compounds were detected including all four tocopherol isoforms with delta- and gamma-tocopherols being predominant (123.35 and 77.80 mu g/100 g, respectively); five organic acids (oxalic, malic, quinic, citric and fumaric acids) with predominant malic acid (3.17 g/100 g); and three phenolic acids and related compounds (p-hydroxybenzoic, p-coumaric and cinnamic acids; 1010, 2420 and 340 mu g/100 g, respectively). M. giganteus methanolic extract exhibited antioxidant activity tested by five different assays with the strongest potential in TBARS assay (EC50 0.31 mg/mL); and antimicrobial activities (MIC/MBC 0.0125-5 mg/mL; MIC/MFC 0.025-0.4 mg/mL). Furthermore, treatment of cervical carcinoma cell line (HeLa) led to reduction in cell's viability in MTT assay (lC(50) 0.41 mg/mL after 48 h), induced process of apoptosis and inhibited cell's migration in vitro. The analysed extract was not toxic for zebrafish embryos (at 0.5 mg/mL), indicating its biosafety and potential application as a dietary supplement in chemoprevention.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Lwt-Food Science and Technology",
title = "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract",
pages = "462-454",
volume = "79",
doi = "10.1016/j.lwt.2017.01.045"
}

Stojković, D. S., Kovačević Grujičić, N., Reis, F. S., Davidović, S., Barros, L., Popović, J., Petrović, I., Pavić, A., Glamoclija, J., Cirić, A., Stevanović, M., Ferreira, I. C. F. R.,& Soković, M.. (2017). Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology
Elsevier Science Bv, Amsterdam., 79, 454-462.
https://doi.org/10.1016/j.lwt.2017.01.045

Stojković DS, Kovačević Grujičić N, Reis FS, Davidović S, Barros L, Popović J, Petrović I, Pavić A, Glamoclija J, Cirić A, Stevanović M, Ferreira ICFR, Soković M. Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract. in Lwt-Food Science and Technology. 2017;79:454-462.
doi:10.1016/j.lwt.2017.01.045 .

Stojković, Dejan S., Kovačević Grujičić, Nataša, Reis, Filipa S., Davidović, Slobodan, Barros, Lillian, Popović, Jelena, Petrović, Isidora, Pavić, Aleksandar, Glamoclija, Jasmina, Cirić, Ana, Stevanović, Milena, Ferreira, Isabel C. F. R., Soković, Marina, "Chemical composition of the mushroom Meripilus giganteus Karst. and bioactive properties of its methanolic extract" in Lwt-Food Science and Technology, 79 (2017):454-462,
https://doi.org/10.1016/j.lwt.2017.01.045 . .

TY  - JOUR
AU  - Smiljković, Marija
AU  - Stanisavljević Ninković, Danijela
AU  - Stojković, Dejan
AU  - Petrović, Isidora
AU  - Vicentić, Jelena Marjanovic
AU  - Popović, Jelena
AU  - Grdadolnik, Simona Golic
AU  - Marković, Dejan
AU  - Sanković-Babić, Snežana
AU  - Glamoclija, Jasmina
AU  - Stevanović, Milena
AU  - Soković, Marina
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1014
AB  - Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.
PB  - EXCLI Journal Managing Office, Dortmund
T2  - EXCLI Journal
T1  - Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions
EP  - 807
SP  - 795
VL  - 16
DO  - 10.17179/excli2017-300
ER  - 

@article{
author = "Smiljković, Marija and Stanisavljević Ninković, Danijela and Stojković, Dejan and Petrović, Isidora and Vicentić, Jelena Marjanovic and Popović, Jelena and Grdadolnik, Simona Golic and Marković, Dejan and Sanković-Babić, Snežana and Glamoclija, Jasmina and Stevanović, Milena and Soković, Marina",
year = "2017",
abstract = "Bioactive potential of apigenin derivative apigenin-7-O-glucoside related to its antifungal activity on Candida spp. and cytotoxic effect on colon cancer cells was studied and compared with bioactive potential of apigenin. Antifungal activity was tested on 14 different isolates of Candida spp. using membrane permeability assay, measuring inhibition of reactive oxidative species and inhibition of CYP51 C. albicans enzyme. Cytotoxic potential of apigenin- 7-O-glucoside was tested on colon cancer HCT116 cells by measuring cell viability, apoptosis rate and apoptosis- and colon cancer-related gene expression. Obtained results indicated considerable antifungal activity of apigenin-7-O-glucoside towards all Candida isolates. Breakdown of C. albicans plasma membrane was achieved upon treatment with apigenin-7-O-glucoside for shorter period of time then with apigenin. Reduction of intra-and extracellular reactive oxidative species was achieved with minimum inhibitory concentrations of both compounds, suggesting that reactive oxidative species inhibition could be a mechanism of antifungal action. None of the compounds exhibited binding affinity to C. albicans CYP51 protein. Besides, apigenin-7-O-glucoside was more effective compared to apigenin in reduction of cell's viability and induction of cell death of HCT116 cells. Treatment with both compounds resulted in chromatin condensation, apoptotic bodies formation and apoptotic genes expression in HCT116 cells, but the apigenin-7-O-glucoside required a lower concentration to achieve the same effect. Compounds apigenin-7-O-glucoside and apigenin displayed prominent antifungal potential and cytotoxic effect on HCT116 cells. However, our results showed that apigenin-7-O-glucoside has more potent activity compared to apigenin in all assays that we used.",
publisher = "EXCLI Journal Managing Office, Dortmund",
journal = "EXCLI Journal",
title = "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions",
pages = "807-795",
volume = "16",
doi = "10.17179/excli2017-300"
}

Smiljković, M., Stanisavljević Ninković, D., Stojković, D., Petrović, I., Vicentić, J. M., Popović, J., Grdadolnik, S. G., Marković, D., Sanković-Babić, S., Glamoclija, J., Stevanović, M.,& Soković, M.. (2017). Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal
EXCLI Journal Managing Office, Dortmund., 16, 795-807.
https://doi.org/10.17179/excli2017-300

Smiljković M, Stanisavljević Ninković D, Stojković D, Petrović I, Vicentić JM, Popović J, Grdadolnik SG, Marković D, Sanković-Babić S, Glamoclija J, Stevanović M, Soković M. Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions. in EXCLI Journal. 2017;16:795-807.
doi:10.17179/excli2017-300 .

Smiljković, Marija, Stanisavljević Ninković, Danijela, Stojković, Dejan, Petrović, Isidora, Vicentić, Jelena Marjanovic, Popović, Jelena, Grdadolnik, Simona Golic, Marković, Dejan, Sanković-Babić, Snežana, Glamoclija, Jasmina, Stevanović, Milena, Soković, Marina, "Apigenin-7-o-glucoside versus apigenin: insight into the modes of anticandidal and cytotoxic actions" in EXCLI Journal, 16 (2017):795-807,
https://doi.org/10.17179/excli2017-300 . .

TY  - JOUR
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Vuković, Vladanka
AU  - Schwirtlich, Marija
AU  - Gredić, Marija
AU  - Stevanović, Milena
AU  - Popović, Jelena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/996
AB  - SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line
IS  - 9
VL  - 12
DO  - 10.1371/journal.pone.0184686
ER  - 

@article{
author = "Stanisavljević Ninković, Danijela and Petrović, Isidora and Vuković, Vladanka and Schwirtlich, Marija and Gredić, Marija and Stevanović, Milena and Popović, Jelena",
year = "2017",
abstract = "SOX14 is a member of the SOX family of transcription factors mainly involved in the regulation of neural development. Recently, it became evident that SOX14 is one of four hyper-methylated genes in cervical carcinoma, considered as a tumor suppressor candidate in this type of malignancy. In this paper we elucidated the role of SOX14 in the regulation of malignant properties of cervical carcinoma cells in vitro. Functional analysis performed in HeLa cells revealed that SOX14 overexpression decreased viability and promoted apoptosis through altering the expression of apoptosis related genes. Our results demonstrated that overexpression of SOX14 initiated accumulation of p53, demonstrating potential cross-talk between SOX14 and the p53 signaling pathway. Further analysis unambiguously showed that SOX14 triggered posttranslational modification of p53 protein, as detected by the significantly increased level of phospho-p53 (Ser-15) in SOX14-overexpressing HeLa cells. Moreover, the obtained results revealed that SOX14 activated p53 protein, which was confirmed by elevated p21 Waf1/Cip1, a well known target gene of p53. This study advances our understanding about the role of SOX14 and might explain the molecular mechanism by which this transcription factor could exert tumor suppressor properties in cervical carcinoma.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line",
number = "9",
volume = "12",
doi = "10.1371/journal.pone.0184686"
}

Stanisavljević Ninković, D., Petrović, I., Vuković, V., Schwirtlich, M., Gredić, M., Stevanović, M.,& Popović, J.. (2017). SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One
Public Library Science, San Francisco., 12(9).
https://doi.org/10.1371/journal.pone.0184686

Stanisavljević Ninković D, Petrović I, Vuković V, Schwirtlich M, Gredić M, Stevanović M, Popović J. SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line. in PLoS One. 2017;12(9).
doi:10.1371/journal.pone.0184686 .

Stanisavljević Ninković, Danijela, Petrović, Isidora, Vuković, Vladanka, Schwirtlich, Marija, Gredić, Marija, Stevanović, Milena, Popović, Jelena, "SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line" in PLoS One, 12, no. 9 (2017),
https://doi.org/10.1371/journal.pone.0184686 . .

TY  - CONF
AU  - Tošić, Nataša
AU  - Virijević, M.
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Davidović, Slobodan
AU  - Suvajdžić-Vuković, Nada
AU  - Pavlović, Sonja
AU  - Stevanović, Milena
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1073
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica-The Hematology Journal
T1  - Prognostic significance of sox2, sox3, sox11, sox14 and sox18 gene expression in de novo acute myeloid leukemia (AML) patients
EP  - 390
SP  - 390
VL  - 102
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1073
ER  - 

@conference{
author = "Tošić, Nataša and Virijević, M. and Petrović, Isidora and Kovačević Grujičić, Nataša and Davidović, Slobodan and Suvajdžić-Vuković, Nada and Pavlović, Sonja and Stevanović, Milena",
year = "2017",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica-The Hematology Journal",
title = "Prognostic significance of sox2, sox3, sox11, sox14 and sox18 gene expression in de novo acute myeloid leukemia (AML) patients",
pages = "390-390",
volume = "102",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1073"
}

Tošić, N., Virijević, M., Petrović, I., Kovačević Grujičić, N., Davidović, S., Suvajdžić-Vuković, N., Pavlović, S.,& Stevanović, M.. (2017). Prognostic significance of sox2, sox3, sox11, sox14 and sox18 gene expression in de novo acute myeloid leukemia (AML) patients. in Haematologica-The Hematology Journal
Ferrata Storti Foundation, Pavia., 102, 390-390.
https://hdl.handle.net/21.15107/rcub_imagine_1073

Tošić N, Virijević M, Petrović I, Kovačević Grujičić N, Davidović S, Suvajdžić-Vuković N, Pavlović S, Stevanović M. Prognostic significance of sox2, sox3, sox11, sox14 and sox18 gene expression in de novo acute myeloid leukemia (AML) patients. in Haematologica-The Hematology Journal. 2017;102:390-390.
https://hdl.handle.net/21.15107/rcub_imagine_1073 .

Tošić, Nataša, Virijević, M., Petrović, Isidora, Kovačević Grujičić, Nataša, Davidović, Slobodan, Suvajdžić-Vuković, Nada, Pavlović, Sonja, Stevanović, Milena, "Prognostic significance of sox2, sox3, sox11, sox14 and sox18 gene expression in de novo acute myeloid leukemia (AML) patients" in Haematologica-The Hematology Journal, 102 (2017):390-390,
https://hdl.handle.net/21.15107/rcub_imagine_1073 .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Popović, Jelena
AU  - Schwirtlich, Marija
AU  - Ranković, Branislava
AU  - Stevanović, Milena
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/836
AB  - Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines
IS  - 11
VL  - 10
DO  - 10.1371/journal.pone.0143591
ER  - 

@article{
author = "Petrović, Isidora and Milivojević, Milena and Popović, Jelena and Schwirtlich, Marija and Ranković, Branislava and Stevanović, Milena",
year = "2015",
abstract = "Although there is much evidence showing functional relationship between Hedgehog pathway, in particular Sonic hedgehog, and SOX transcription factors during embryonic development, scarce data are available regarding their crosstalk in cancer cells. SOX18 protein plays an important role in promoting tumor angiogenesis and therefore emerged as a promising potential target in antiangiogenic tumor therapy. Recently it became evident that expression of SOX18 gene in tumors is not restricted to endothelium of accompanying blood and lymphatic vessels, but in tumor cells as well. In this paper we have identified human SOX18 gene as a novel target gene of Hedgehog signaling in cervical carcinoma cell lines. We have presented data showing that expression of SOX18 gene is regulated by GLI1 and GLI2 transcription factors, final effectors of Hedgehog signaling, and that modulation of Hedgehog signaling activity in considerably influence SOX18 expression. We consider important that Hedgehog pathway inhibitors reduced SOX18 expression, thus showing, for the first time, possibility for manipulationwith SOX18 gene expression. In addition, we analyzed the role of SOX18 in malignant potential of cervical carcinoma cell line, and showed that its overexpression has no influence on cells proliferation and viability, but substantially promotes migration and invasion of cells in vitro. Pro-migratory effect of SOX18 suggests its role in promoting malignant spreading, possibly in response to Hedgehog activation.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines",
number = "11",
volume = "10",
doi = "10.1371/journal.pone.0143591"
}

Petrović, I., Milivojević, M., Popović, J., Schwirtlich, M., Ranković, B.,& Stevanović, M.. (2015). SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One
Public Library Science, San Francisco., 10(11).
https://doi.org/10.1371/journal.pone.0143591

Petrović I, Milivojević M, Popović J, Schwirtlich M, Ranković B, Stevanović M. SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines. in PLoS One. 2015;10(11).
doi:10.1371/journal.pone.0143591 .

Petrović, Isidora, Milivojević, Milena, Popović, Jelena, Schwirtlich, Marija, Ranković, Branislava, Stevanović, Milena, "SOX18 Is a Novel Target Gene of Hedgehog Signaling in Cervical Carcinoma Cell Lines" in PLoS One, 10, no. 11 (2015),
https://doi.org/10.1371/journal.pone.0143591 . .

TY  - CONF
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Stojković, D.
AU  - Davidović, Slobodan
AU  - Glamoclija, J.
AU  - Cirić, A.
AU  - Soković, M.
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/729
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - Meripilus giganteus in prevention of cancer: Phenolic profile, biological potential and antitumor effect via upregulation of p53 and SOX1 expression in HeLa cells
EP  - S194
SP  - S194
VL  - 50
DO  - 10.1016/S0959-8049(14)50709-5
ER  - 

@conference{
author = "Petrović, Isidora and Kovačević Grujičić, Nataša and Stojković, D. and Davidović, Slobodan and Glamoclija, J. and Cirić, A. and Soković, M. and Stevanović, Milena",
year = "2014",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "Meripilus giganteus in prevention of cancer: Phenolic profile, biological potential and antitumor effect via upregulation of p53 and SOX1 expression in HeLa cells",
pages = "S194-S194",
volume = "50",
doi = "10.1016/S0959-8049(14)50709-5"
}

Petrović, I., Kovačević Grujičić, N., Stojković, D., Davidović, S., Glamoclija, J., Cirić, A., Soković, M.,& Stevanović, M.. (2014). Meripilus giganteus in prevention of cancer: Phenolic profile, biological potential and antitumor effect via upregulation of p53 and SOX1 expression in HeLa cells. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 50, S194-S194.
https://doi.org/10.1016/S0959-8049(14)50709-5

Petrović I, Kovačević Grujičić N, Stojković D, Davidović S, Glamoclija J, Cirić A, Soković M, Stevanović M. Meripilus giganteus in prevention of cancer: Phenolic profile, biological potential and antitumor effect via upregulation of p53 and SOX1 expression in HeLa cells. in European Journal of Cancer. 2014;50:S194-S194.
doi:10.1016/S0959-8049(14)50709-5 .

Petrović, Isidora, Kovačević Grujičić, Nataša, Stojković, D., Davidović, Slobodan, Glamoclija, J., Cirić, A., Soković, M., Stevanović, Milena, "Meripilus giganteus in prevention of cancer: Phenolic profile, biological potential and antitumor effect via upregulation of p53 and SOX1 expression in HeLa cells" in European Journal of Cancer, 50 (2014):S194-S194,
https://doi.org/10.1016/S0959-8049(14)50709-5 . .

TY  - JOUR
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Popović, Jelena
AU  - Petrović, Isidora
AU  - Topalović, Vladanka
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/723
AB  - SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.
PB  - Korean Society Biochemistry & Molecular Biology, Seoul
T2  - BMB Reports
T1  - Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells
EP  - 202
IS  - 4
SP  - 197
VL  - 47
DO  - 10.5483/BMBRep.2014.47.4.084
ER  - 

@article{
author = "Kovačević Grujičić, Nataša and Mojsin, Marija and Popović, Jelena and Petrović, Isidora and Topalović, Vladanka and Stevanović, Milena",
year = "2014",
abstract = "SOX3 is one of the earliest neural markers in vertebrates, playing the role in specifying neuronal fate. In this study we have established first functional link between CREB and human SOX3 gene which both have important roles in the nervous system throughout development and in the adulthood. Here we demonstrate both in vitro and in vivo that CREB binds to CRE half-site located -195 to -191 within the human SOX3 promoter. Overexpression studies with CREB or its dominant-negative inhibitor A-CREB indicate that this transcription factor acts as a positive regulator of basal SOX3 gene expression in NT2/D1 cells. This is further confirmed by mutational analysis where mutation of CREB binding site results in reduction of SOX3 promoter activity. Our results point at CREB as a positive regulator of SOX3 gene transcription in NT2/D1 cells, while its contribution to RA induction of SOX3 promoter is not prominent.",
publisher = "Korean Society Biochemistry & Molecular Biology, Seoul",
journal = "BMB Reports",
title = "Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells",
pages = "202-197",
number = "4",
volume = "47",
doi = "10.5483/BMBRep.2014.47.4.084"
}

Kovačević Grujičić, N., Mojsin, M., Popović, J., Petrović, I., Topalović, V.,& Stevanović, M.. (2014). Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells. in BMB Reports
Korean Society Biochemistry & Molecular Biology, Seoul., 47(4), 197-202.
https://doi.org/10.5483/BMBRep.2014.47.4.084

Kovačević Grujičić N, Mojsin M, Popović J, Petrović I, Topalović V, Stevanović M. Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells. in BMB Reports. 2014;47(4):197-202.
doi:10.5483/BMBRep.2014.47.4.084 .

Kovačević Grujičić, Nataša, Mojsin, Marija, Popović, Jelena, Petrović, Isidora, Topalović, Vladanka, Stevanović, Milena, "Cyclic AMP response element binding (CREB) protein acts as a positive regulator of SOX3 gene expression in NT2/D1 cells" in BMB Reports, 47, no. 4 (2014):197-202,
https://doi.org/10.5483/BMBRep.2014.47.4.084 . .

TY  - CONF
AU  - Petrović, Isidora
AU  - Popović, Jelena
AU  - Stanisavljević Ninković, Danijela
AU  - Schwirtlich, Marija
AU  - Klajn, Andrijana
AU  - Marjanović, J.
AU  - Kovačević Grujičić, Nataša
AU  - Topalović, Vladanka
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/731
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - SOX14 downregulates SOX1 expression in HeLa cells
EP  - S56
SP  - S56
VL  - 50
DO  - 10.1016/S0959-8049(14)50210-9
ER  - 

@conference{
author = "Petrović, Isidora and Popović, Jelena and Stanisavljević Ninković, Danijela and Schwirtlich, Marija and Klajn, Andrijana and Marjanović, J. and Kovačević Grujičić, Nataša and Topalović, Vladanka and Mojsin, Marija and Stevanović, Milena",
year = "2014",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "SOX14 downregulates SOX1 expression in HeLa cells",
pages = "S56-S56",
volume = "50",
doi = "10.1016/S0959-8049(14)50210-9"
}

Petrović, I., Popović, J., Stanisavljević Ninković, D., Schwirtlich, M., Klajn, A., Marjanović, J., Kovačević Grujičić, N., Topalović, V., Mojsin, M.,& Stevanović, M.. (2014). SOX14 downregulates SOX1 expression in HeLa cells. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 50, S56-S56.
https://doi.org/10.1016/S0959-8049(14)50210-9

Petrović I, Popović J, Stanisavljević Ninković D, Schwirtlich M, Klajn A, Marjanović J, Kovačević Grujičić N, Topalović V, Mojsin M, Stevanović M. SOX14 downregulates SOX1 expression in HeLa cells. in European Journal of Cancer. 2014;50:S56-S56.
doi:10.1016/S0959-8049(14)50210-9 .

Petrović, Isidora, Popović, Jelena, Stanisavljević Ninković, Danijela, Schwirtlich, Marija, Klajn, Andrijana, Marjanović, J., Kovačević Grujičić, Nataša, Topalović, Vladanka, Mojsin, Marija, Stevanović, Milena, "SOX14 downregulates SOX1 expression in HeLa cells" in European Journal of Cancer, 50 (2014):S56-S56,
https://doi.org/10.1016/S0959-8049(14)50210-9 . .

TY  - CONF
AU  - Petrović, Isidora
AU  - Milivojević, Milena
AU  - Mojsin, Marija
AU  - Drakulić, Danijela
AU  - Kovačević Grujičić, Nataša
AU  - Topalović, Vladanka
AU  - Davidović, Slobodan
AU  - Stevanović, Milena
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/730
PB  - Elsevier Sci Ltd, Oxford
C3  - European Journal of Cancer
T1  - The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line
EP  - S120
SP  - S120
VL  - 50
DO  - 10.1016/S0959-8049(14)50444-3
ER  - 

@conference{
author = "Petrović, Isidora and Milivojević, Milena and Mojsin, Marija and Drakulić, Danijela and Kovačević Grujičić, Nataša and Topalović, Vladanka and Davidović, Slobodan and Stevanović, Milena",
year = "2014",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "European Journal of Cancer",
title = "The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line",
pages = "S120-S120",
volume = "50",
doi = "10.1016/S0959-8049(14)50444-3"
}

Petrović, I., Milivojević, M., Mojsin, M., Drakulić, D., Kovačević Grujičić, N., Topalović, V., Davidović, S.,& Stevanović, M.. (2014). The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line. in European Journal of Cancer
Elsevier Sci Ltd, Oxford., 50, S120-S120.
https://doi.org/10.1016/S0959-8049(14)50444-3

Petrović I, Milivojević M, Mojsin M, Drakulić D, Kovačević Grujičić N, Topalović V, Davidović S, Stevanović M. The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line. in European Journal of Cancer. 2014;50:S120-S120.
doi:10.1016/S0959-8049(14)50444-3 .

Petrović, Isidora, Milivojević, Milena, Mojsin, Marija, Drakulić, Danijela, Kovačević Grujičić, Nataša, Topalović, Vladanka, Davidović, Slobodan, Stevanović, Milena, "The role of Hedgehog signaling pathway in the regulation of SOX18 gene expression in cervical carcinoma cell line" in European Journal of Cancer, 50 (2014):S120-S120,
https://doi.org/10.1016/S0959-8049(14)50444-3 . .

TY  - JOUR
AU  - Milivojević, Milena
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Popović, Jelena
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/672
AB  - SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.
PB  - Maik Nauka/Interperiodica/Springer, New York
T2  - Biochemistry-Moscow
T1  - Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein
EP  - 1292
IS  - 11
SP  - 1287
VL  - 78
DO  - 10.1134/S0006297913110096
ER  - 

@article{
author = "Milivojević, Milena and Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Mojsin, Marija and Stevanović, Milena",
year = "2013",
abstract = "SOX18 transcription factor plays important roles in a range of biological processes such as vasculogenesis, hair follicle development, lymphangiogenesis, atherosclerosis, and angiogenesis. In this paper we present the generation of a novel SOX18 dominant-negative mutant (SOX18DN) encoding truncated SOX18 protein that lacks a trans-activation domain. We show that both wild-type SOX18 (SOX18wt) and truncated human SOX18 proteins are able to bind to their consensus sequence in vitro. Functional analysis confirmed that SOX18wt has potent trans-activation properties, while SOX18DN displays dominant-negative effect. We believe that these SOX18wt and SOX18DN expression constructs could be successfully used for further characterization of the function of this protein.",
publisher = "Maik Nauka/Interperiodica/Springer, New York",
journal = "Biochemistry-Moscow",
title = "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein",
pages = "1292-1287",
number = "11",
volume = "78",
doi = "10.1134/S0006297913110096"
}

Milivojević, M., Petrović, I., Kovačević Grujičić, N., Popović, J., Mojsin, M.,& Stevanović, M.. (2013). Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow
Maik Nauka/Interperiodica/Springer, New York., 78(11), 1287-1292.
https://doi.org/10.1134/S0006297913110096

Milivojević M, Petrović I, Kovačević Grujičić N, Popović J, Mojsin M, Stevanović M. Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein. in Biochemistry-Moscow. 2013;78(11):1287-1292.
doi:10.1134/S0006297913110096 .

Milivojević, Milena, Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Mojsin, Marija, Stevanović, Milena, "Construction and functional analysis of novel dominant-negative mutant of human SOX18 protein" in Biochemistry-Moscow, 78, no. 11 (2013):1287-1292,
https://doi.org/10.1134/S0006297913110096 . .