TY  - JOUR
AU  - Dragojević, Milica
AU  - Stanković, Nada
AU  - Đokić, Lidija
AU  - Raičević, Vera
AU  - Jovičić-Petrović, Jelena
PY  - 2023
UR  - https://doi.org/10.1186/s40793-023-00477-x
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1796
AB  - The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils.
T2  - Environmental Microbiome
T1  - Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria
IS  - 1
SP  - 20
VL  - 18
DO  - 10.1186/s40793-023-00477-x
ER  - 

@article{
author = "Dragojević, Milica and Stanković, Nada and Đokić, Lidija and Raičević, Vera and Jovičić-Petrović, Jelena",
year = "2023",
abstract = "The adaptability of halophytes to increased soil salinity is related to complex rhizosphere interactions. In this study, an integrative approach, combining culture-independent and culture-dependent techniques was used to analyze the bacterial communities in the endorizosphere of indigenous succulent halophytes Salicornia europaea, Suaeda maritima, and Camphorosma annua from the natural salt marshes of Slano Kopovo (Serbia). The 16 S rDNA analyses gave, for the first time, an insight into the composition of the endophytic bacterial communities of S. maritima and C. annua. We have found that the composition of endophyte microbiomes in the same habitat is to some extent influenced by plant species. A cultivable portion of the halophyte microbiota was tested at different NaCl concentrations for the set of plant growth promoting (PGP) traits. Through the mining of indigenous halotolerant endophytes, we obtained a collection representing a core endophyte microbiome conferring desirable PGP traits. The majority (65%) of the selected strains belonged to the common halotolerant/halophilic genera Halomonas, Kushneria, and Halobacillus, with representatives exhibiting multiple PGP traits, and retaining beneficial traits in conditions of the increased salinity. The results suggest that the root endosphere of halophytes is a valuable source of PGP bacteria supporting plant growth and fitness in salt-affected soils.",
journal = "Environmental Microbiome",
title = "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria",
number = "1",
pages = "20",
volume = "18",
doi = "10.1186/s40793-023-00477-x"
}

Dragojević, M., Stanković, N., Đokić, L., Raičević, V.,& Jovičić-Petrović, J.. (2023). Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome, 18(1), 20.
https://doi.org/10.1186/s40793-023-00477-x

Dragojević M, Stanković N, Đokić L, Raičević V, Jovičić-Petrović J. Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria. in Environmental Microbiome. 2023;18(1):20.
doi:10.1186/s40793-023-00477-x .

Dragojević, Milica, Stanković, Nada, Đokić, Lidija, Raičević, Vera, Jovičić-Petrović, Jelena, "Endorhizosphere of indigenous succulent halophytes: a valuable resource of plant growth promoting bacteria" in Environmental Microbiome, 18, no. 1 (2023):20,
https://doi.org/10.1186/s40793-023-00477-x . .

TY  - JOUR
AU  - Salević-Jelić, Ana
AU  - Lević, Steva
AU  - Stojanović, Dušica
AU  - Jeremić, Sanja
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Ignjatović, Ivana Sredović
AU  - Uskoković, Petar
AU  - Nedović, Viktor
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2214289423000042
UR  - https://doi.org/10.1016/j.fpsl.2023.101027
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1921
AB  - This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).
T2  - Food Packaging and Shelf Life
T2  - Food Packaging and Shelf LifeFood Packaging and Shelf Life
T1  - Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization
SP  - 101027
VL  - 35
DO  - 10.1016/j.fpsl.2023.101027
ER  - 

@article{
author = "Salević-Jelić, Ana and Lević, Steva and Stojanović, Dušica and Jeremić, Sanja and Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Ignjatović, Ivana Sredović and Uskoković, Petar and Nedović, Viktor",
year = "2023",
abstract = "This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).",
journal = "Food Packaging and Shelf Life, Food Packaging and Shelf LifeFood Packaging and Shelf Life",
title = "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization",
pages = "101027",
volume = "35",
doi = "10.1016/j.fpsl.2023.101027"
}

Salević-Jelić, A., Lević, S., Stojanović, D., Jeremić, S., Miletić, D., Pantić, M., Pavlović, V., Ignjatović, I. S., Uskoković, P.,& Nedović, V.. (2023). Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life, 35, 101027.
https://doi.org/10.1016/j.fpsl.2023.101027

Salević-Jelić A, Lević S, Stojanović D, Jeremić S, Miletić D, Pantić M, Pavlović V, Ignjatović IS, Uskoković P, Nedović V. Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life. 2023;35:101027.
doi:10.1016/j.fpsl.2023.101027 .

Salević-Jelić, Ana, Lević, Steva, Stojanović, Dušica, Jeremić, Sanja, Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Ignjatović, Ivana Sredović, Uskoković, Petar, Nedović, Viktor, "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization" in Food Packaging and Shelf Life, 35 (2023):101027,
https://doi.org/10.1016/j.fpsl.2023.101027 . .

TY  - JOUR
AU  - Salević-Jelić, Ana
AU  - Lević, Steva
AU  - Stojanović, Dušica
AU  - Jeremić, Sanja
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Ignjatović, Ivana Sredović
AU  - Uskoković, Petar
AU  - Nedović, Viktor
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2214289423000042
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1887
UR  - https://doi.org/10.1016/j.fpsl.2023.101027
AB  - This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).
T2  - Food Packaging and Shelf Life
T2  - Food Packaging and Shelf LifeFood Packaging and Shelf Life
T1  - Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization
SP  - 101027
VL  - 35
DO  - 10.1016/j.fpsl.2023.101027
ER  - 

@article{
author = "Salević-Jelić, Ana and Lević, Steva and Stojanović, Dušica and Jeremić, Sanja and Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Ignjatović, Ivana Sredović and Uskoković, Petar and Nedović, Viktor",
year = "2023",
abstract = "This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).",
journal = "Food Packaging and Shelf Life, Food Packaging and Shelf LifeFood Packaging and Shelf Life",
title = "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization",
pages = "101027",
volume = "35",
doi = "10.1016/j.fpsl.2023.101027"
}

Salević-Jelić, A., Lević, S., Stojanović, D., Jeremić, S., Miletić, D., Pantić, M., Pavlović, V., Ignjatović, I. S., Uskoković, P.,& Nedović, V.. (2023). Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life, 35, 101027.
https://doi.org/10.1016/j.fpsl.2023.101027

Salević-Jelić A, Lević S, Stojanović D, Jeremić S, Miletić D, Pantić M, Pavlović V, Ignjatović IS, Uskoković P, Nedović V. Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life. 2023;35:101027.
doi:10.1016/j.fpsl.2023.101027 .

Salević-Jelić, Ana, Lević, Steva, Stojanović, Dušica, Jeremić, Sanja, Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Ignjatović, Ivana Sredović, Uskoković, Petar, Nedović, Viktor, "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization" in Food Packaging and Shelf Life, 35 (2023):101027,
https://doi.org/10.1016/j.fpsl.2023.101027 . .

TY  - JOUR
AU  - Kljajević, Nemanja
AU  - Miloradović, Zorana
AU  - Miočinović, Jelena
AU  - Pavlović, Vladimir
AU  - Tomić, Nikola
AU  - Jovanović, Snežana
PY  - 2023
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/1471-0307.13042
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2328
AB  - Long-term frozen storage of caprine milk is an important way of overcoming seasonal production. White brined cheese, matured for 56 days, was made from frozen-stored caprine milk. Several properties of experimental and control cheese variants were investigated throughout the maturation period: physico-chemical, sensory, proteolytic (by urea-PAGE electrophoresis), texture attributes, microstructure (by scanning electron microscopy). Most of investigated properties were influenced by frozen storage and maturation period, but differences were less pronounced on the 56th day of maturation. Moreover, sensory analysis showed that both cheese variants were well accepted by consumers.
PB  - Wiley
T2  - International Journal of Dairy Technology
T2  - International Journal of Dairy Technology
T1  - Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk
IS  - n/a
VL  - n/a
DO  - 10.1111/1471-0307.13042
ER  - 

@article{
author = "Kljajević, Nemanja and Miloradović, Zorana and Miočinović, Jelena and Pavlović, Vladimir and Tomić, Nikola and Jovanović, Snežana",
year = "2023",
abstract = "Long-term frozen storage of caprine milk is an important way of overcoming seasonal production. White brined cheese, matured for 56 days, was made from frozen-stored caprine milk. Several properties of experimental and control cheese variants were investigated throughout the maturation period: physico-chemical, sensory, proteolytic (by urea-PAGE electrophoresis), texture attributes, microstructure (by scanning electron microscopy). Most of investigated properties were influenced by frozen storage and maturation period, but differences were less pronounced on the 56th day of maturation. Moreover, sensory analysis showed that both cheese variants were well accepted by consumers.",
publisher = "Wiley",
journal = "International Journal of Dairy Technology, International Journal of Dairy Technology",
title = "Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk",
number = "n/a",
volume = "n/a",
doi = "10.1111/1471-0307.13042"
}

Kljajević, N., Miloradović, Z., Miočinović, J., Pavlović, V., Tomić, N.,& Jovanović, S.. (2023). Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk. in International Journal of Dairy Technology
Wiley., n/a(n/a).
https://doi.org/10.1111/1471-0307.13042

Kljajević N, Miloradović Z, Miočinović J, Pavlović V, Tomić N, Jovanović S. Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk. in International Journal of Dairy Technology. 2023;n/a(n/a).
doi:10.1111/1471-0307.13042 .

Kljajević, Nemanja, Miloradović, Zorana, Miočinović, Jelena, Pavlović, Vladimir, Tomić, Nikola, Jovanović, Snežana, "Insights into proteolysis, textural evolution, microstructural changes and sensory attributes of white brined cheese from frozen-stored caprine milk" in International Journal of Dairy Technology, n/a, no. n/a (2023),
https://doi.org/10.1111/1471-0307.13042 . .

TY  - DATA
AU  - Milinčić, Danijel
AU  - Stanisavljević, Nemanja
AU  - Kostić, Aleksandar
AU  - Gašić, Uroš
AU  - Stanojević, Slađana
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/11/2164
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2064
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2071
T2  - Antioxidants
T1  - Supplementary data for the article: Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants, 11(11), 2164. https://doi.org/10.3390/antiox11112164
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2071
ER  - 

@misc{
author = "Milinčić, Danijel and Stanisavljević, Nemanja and Kostić, Aleksandar and Gašić, Uroš and Stanojević, Slađana and Tešić, Živoslav and Pešić, Mirjana",
year = "2022",
journal = "Antioxidants",
title = "Supplementary data for the article: Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants, 11(11), 2164. https://doi.org/10.3390/antiox11112164",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2071"
}

Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Supplementary data for the article: Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants, 11(11), 2164. https://doi.org/10.3390/antiox11112164. in Antioxidants.
https://hdl.handle.net/21.15107/rcub_imagine_2071

Milinčić D, Stanisavljević N, Kostić A, Gašić U, Stanojević S, Tešić Ž, Pešić M. Supplementary data for the article: Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants, 11(11), 2164. https://doi.org/10.3390/antiox11112164. in Antioxidants. 2022;.
https://hdl.handle.net/21.15107/rcub_imagine_2071 .

Milinčić, Danijel, Stanisavljević, Nemanja, Kostić, Aleksandar, Gašić, Uroš, Stanojević, Slađana, Tešić, Živoslav, Pešić, Mirjana, "Supplementary data for the article: Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants, 11(11), 2164. https://doi.org/10.3390/antiox11112164" in Antioxidants (2022),
https://hdl.handle.net/21.15107/rcub_imagine_2071 .

TY  - CONF
AU  - Živanović, Bojana
AU  - Prokić, Ljiljana
AU  - Milić Komić, Sonja
AU  - Nikolić, Nenad
AU  - Sedlarević Zorić, Ana
AU  - Vidović, Marija
AU  - Veljović Jovanović, Sonja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1842
AB  - The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.
PB  - Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade
C3  - 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
T1  - Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca
SP  - 83
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1842
ER  - 

@conference{
author = "Živanović, Bojana and Prokić, Ljiljana and Milić Komić, Sonja and Nikolić, Nenad and Sedlarević Zorić, Ana and Vidović, Marija and Veljović Jovanović, Sonja",
year = "2022",
abstract = "The objective of this study was to determine the constitutive differences in physiological, biochemical and morphological parameters between two tomato genotypes with different levels of abscisic acid (ABA) – wild type Ailsa Craig (WT) and ABA deficient mutant flacca. Due to reduced ABA biosynthesis, flacca mutant is a suitable model system for investigating the influence of endogenous leaf ABA level in response to drought in plants. Within this research, plants were grown under controlled conditions at 800 μmol m-2s-1, until the end of the vegetative phase when samples were taken. The accumulation of the most abundant soluble sugars, sorbitol, phenolic compounds, and ascorbate in the leaves, as well as the cell walls compounds, were analyzed. Lower constitutive ABA content in flacca was accompanied by two times higher stomatal conductance and similar leaf water potential. Higher content of phenolic compounds (HBAs, HCAs, flavonoids) was determined in WT plants, which was in contrast with the elevated accumulation of the epidermal flavonoids in flacca. Larger accumulation of sorbitol in WT, and of the most abundant soluble sugars (glucose, fructose and sucrose) indicate that flacca accumulates lower content of osmolytes which was opposite to the condition at low light. However, an alternative mechanism related to cell wall modulation imposed its importance in the development of plant acclimation mechanisms under stressful environmental conditions in tomato deficient in ABA. On the other hand, an elevated ascorbate redox state in flacca indicates a higher sensitivity to oxidative stress of the mutant compared to WT even in optimal environmental conditions.",
publisher = "Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade",
journal = "4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade",
title = "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca",
pages = "83",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1842"
}

Živanović, B., Prokić, L., Milić Komić, S., Nikolić, N., Sedlarević Zorić, A., Vidović, M.,& Veljović Jovanović, S.. (2022). Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade
Serbian Plant Physiology Society Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade Faculty of Biology, University of Belgrade., 83.
https://hdl.handle.net/21.15107/rcub_imagine_1842

Živanović B, Prokić L, Milić Komić S, Nikolić N, Sedlarević Zorić A, Vidović M, Veljović Jovanović S. Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca. in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade. 2022;:83.
https://hdl.handle.net/21.15107/rcub_imagine_1842 .

Živanović, Bojana, Prokić, Ljiljana, Milić Komić, Sonja, Nikolić, Nenad, Sedlarević Zorić, Ana, Vidović, Marija, Veljović Jovanović, Sonja, "Comparative study of physiological, biochemical and morphological parameters in two tomato genotypes, wild type cv. Ailsa Craig and its ABA-deficient mutant flacca" in 4th International Conference on Plant Biology and 23rd SPPS Meeting, 6-8 October 2022, Belgrade (2022):83,
https://hdl.handle.net/21.15107/rcub_imagine_1842 .

TY  - JOUR
AU  - Gasić, Katarina
AU  - Obradović, Mina
AU  - Kuzmanović, Nemanja
AU  - Zlatković, Nevena
AU  - Ivanović, Milan
AU  - Ristić, Danijela
AU  - Obradović, Aleksa
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1571
AB  - Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67 degrees C. They were stable at pH 5-9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli
VL  - 12
DO  - 10.3389/fmicb.2021.803789
ER  - 

@article{
author = "Gasić, Katarina and Obradović, Mina and Kuzmanović, Nemanja and Zlatković, Nevena and Ivanović, Milan and Ristić, Danijela and Obradović, Aleksa",
year = "2022",
abstract = "Bacterial fruit blotch and seedling blight, caused by Acidovorax citrulli, is one of the most destructive diseases of melon and watermelon in many countries. Pathogen-free seed and cultural practices are major pillars of the disease control. However, use of bacteriophages as natural biocontrol agents might also contribute to the disease management. Therefore, we isolated 12 bacteriophages specific to A. citrulli, from phyllosphere and rhizosphere of diseased watermelon plants. The phage strains were characterized based on their host range, plaque and virion morphology, thermal inactivation point, adsorption rate, one step growth curve, restriction fragment length polymorphism (RFLP), and genomic analysis. Transmission electron microscopy of three phage strains indicated that they belong to the order Caudovirales, family Siphoviridae. All phages lysed 30 out of 32 tested A. citrulli strains isolated in Serbia, and did not lyse other less related bacterial species. They produced clear plaques, 2 mm in diameter, on bacterial lawns of different A. citrulli strains after 24 h of incubation. The thermal inactivation point was 66 or 67 degrees C. They were stable at pH 5-9, but were sensitive to chloroform and inactivated in either 5 or 10 min exposure to ultraviolet (UV) light. RFLP analysis using EcoRI, BsmI and BamHI enzymes did not show genetic differences among the tested phages. Adsorption rate and one step growth curve were determined for the Acidovorax phage ACF1. Draft genome sequence of the ACF1 phage was 59.377 bp in size, with guanine-cytosine (GC) content 64.5%, including 89 open reading frames. This phage shared a very high genomic identity with Acidovorax phage ACPWH, isolated in South Korea. Evaluation of systemic nature of ACF1 strain showed that it can be absorbed by roots and translocated to upper parts of watermelon plants where it survived up to 10 days.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli",
volume = "12",
doi = "10.3389/fmicb.2021.803789"
}

Gasić, K., Obradović, M., Kuzmanović, N., Zlatković, N., Ivanović, M., Ristić, D.,& Obradović, A.. (2022). Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 12.
https://doi.org/10.3389/fmicb.2021.803789

Gasić K, Obradović M, Kuzmanović N, Zlatković N, Ivanović M, Ristić D, Obradović A. Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli. in Frontiers in Microbiology. 2022;12.
doi:10.3389/fmicb.2021.803789 .

Gasić, Katarina, Obradović, Mina, Kuzmanović, Nemanja, Zlatković, Nevena, Ivanović, Milan, Ristić, Danijela, Obradović, Aleksa, "Isolation, Characterization and Draft Genome Analysis of Bacteriophages Infecting Acidovorax citrulli" in Frontiers in Microbiology, 12 (2022),
https://doi.org/10.3389/fmicb.2021.803789 . .

TY  - JOUR
AU  - Matijašević, Danka
AU  - Pantić, Milena
AU  - Stanisavljević, Nemanja
AU  - Jevtić, Sanja
AU  - Rajić, Nevenka
AU  - Lević, Steva
AU  - Nedović, Viktor
AU  - Nikšić, Miomir
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1565
AB  - Research background. In the recent years, considerable attention has been given to selenium status since its deficiency is linked with various disorders and affects at least 13 % of world population. Additionally, mushrooms are known to possess pronounced capacity for absorption of various micronutrients, including Se, from soil/substrate. Here, we investigate the possibility of using Se-rich zeolitic tuff as a supplement for production of selenized mushroom. Furthermore, the impact of the enrichment on the activity of antioxidant enzymes and biological potential of Coriolus versicolor medicinal mushroom is studied. Experimental approach. Se(IV)- and Se(VI)-modified natural zeolitic tuff from the Serbian deposit Zlatokop was used as supplement for mushroom cultivation. To examine the effectiveness of selenium enrichment, we determined total selenium with inductively coupled plasma mass spectrometry (ICP-MS), together with the activity of antioxidant enzymes in fresh fruiting bodies and biological potential of methanolic extracts. Antioxidant activity was evaluated using the appropriate tests for: inhibition of lipid peroxidation, DPPH free radical scavenging assay, Fe(III)-reducing antioxidant power assay and ability of chelating Fe2+ ions. The antibacterial activity against foodborne pathogens was measured by broth microdilution assay. Additionally, chemical composition of the prepared extracts was studied using UV-Vis and Fourier transform infrared (FTIR) spectroscopy. Results and conclusions. Content of selenium detected in biofortified C. versicolor was even 470 times higher than in control on dry mass basis ((140.7 +/- 3.8) vs (0.3 +/- 0.1) mu g/g), proving that Se-rich zeolitic tuff is an excellent supplement for mushroom production. Furthermore, the results of monitoring the activity of antioxidant enzymes revealed that most of the Se-enriched mushrooms exhibited higher superoxide dismutase (SOD) and cata lase (CAT) and lower glutathione peroxidase (GSH-Px) activities than control. Due to higher amounts of enzymes, which can quickly catalyze the reduction of superoxide radicals, the quality of selenium-enriched mushrooms is preserved for a longer period of time. Investigation of biological potential indicated that Se-enriched mushroom methanolic extracts, generally, expressed enhanced antioxidant properties. Additionally, extracts showed antibacterial activity against all tested pathogenic microorganisms. Novelty and scientific contribution. Cultivation of mushrooms on Se-enriched zeolitic tuff is a new technological approach for obtaining Se-fortified food/supplements with enhanced antioxidant and antibacterial activities.
PB  - Faculty Food Technology Biotechnology, Zagreb
T2  - Food Technology and Biotechnology
T1  - Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement
EP  - 79
IS  - 1
SP  - 67
VL  - 60
DO  - 10.17113/ftb.60.01.22.7172
ER  - 

@article{
author = "Matijašević, Danka and Pantić, Milena and Stanisavljević, Nemanja and Jevtić, Sanja and Rajić, Nevenka and Lević, Steva and Nedović, Viktor and Nikšić, Miomir",
year = "2022",
abstract = "Research background. In the recent years, considerable attention has been given to selenium status since its deficiency is linked with various disorders and affects at least 13 % of world population. Additionally, mushrooms are known to possess pronounced capacity for absorption of various micronutrients, including Se, from soil/substrate. Here, we investigate the possibility of using Se-rich zeolitic tuff as a supplement for production of selenized mushroom. Furthermore, the impact of the enrichment on the activity of antioxidant enzymes and biological potential of Coriolus versicolor medicinal mushroom is studied. Experimental approach. Se(IV)- and Se(VI)-modified natural zeolitic tuff from the Serbian deposit Zlatokop was used as supplement for mushroom cultivation. To examine the effectiveness of selenium enrichment, we determined total selenium with inductively coupled plasma mass spectrometry (ICP-MS), together with the activity of antioxidant enzymes in fresh fruiting bodies and biological potential of methanolic extracts. Antioxidant activity was evaluated using the appropriate tests for: inhibition of lipid peroxidation, DPPH free radical scavenging assay, Fe(III)-reducing antioxidant power assay and ability of chelating Fe2+ ions. The antibacterial activity against foodborne pathogens was measured by broth microdilution assay. Additionally, chemical composition of the prepared extracts was studied using UV-Vis and Fourier transform infrared (FTIR) spectroscopy. Results and conclusions. Content of selenium detected in biofortified C. versicolor was even 470 times higher than in control on dry mass basis ((140.7 +/- 3.8) vs (0.3 +/- 0.1) mu g/g), proving that Se-rich zeolitic tuff is an excellent supplement for mushroom production. Furthermore, the results of monitoring the activity of antioxidant enzymes revealed that most of the Se-enriched mushrooms exhibited higher superoxide dismutase (SOD) and cata lase (CAT) and lower glutathione peroxidase (GSH-Px) activities than control. Due to higher amounts of enzymes, which can quickly catalyze the reduction of superoxide radicals, the quality of selenium-enriched mushrooms is preserved for a longer period of time. Investigation of biological potential indicated that Se-enriched mushroom methanolic extracts, generally, expressed enhanced antioxidant properties. Additionally, extracts showed antibacterial activity against all tested pathogenic microorganisms. Novelty and scientific contribution. Cultivation of mushrooms on Se-enriched zeolitic tuff is a new technological approach for obtaining Se-fortified food/supplements with enhanced antioxidant and antibacterial activities.",
publisher = "Faculty Food Technology Biotechnology, Zagreb",
journal = "Food Technology and Biotechnology",
title = "Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement",
pages = "79-67",
number = "1",
volume = "60",
doi = "10.17113/ftb.60.01.22.7172"
}

Matijašević, D., Pantić, M., Stanisavljević, N., Jevtić, S., Rajić, N., Lević, S., Nedović, V.,& Nikšić, M.. (2022). Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement. in Food Technology and Biotechnology
Faculty Food Technology Biotechnology, Zagreb., 60(1), 67-79.
https://doi.org/10.17113/ftb.60.01.22.7172

Matijašević D, Pantić M, Stanisavljević N, Jevtić S, Rajić N, Lević S, Nedović V, Nikšić M. Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement. in Food Technology and Biotechnology. 2022;60(1):67-79.
doi:10.17113/ftb.60.01.22.7172 .

Matijašević, Danka, Pantić, Milena, Stanisavljević, Nemanja, Jevtić, Sanja, Rajić, Nevenka, Lević, Steva, Nedović, Viktor, Nikšić, Miomir, "Coriolus versicolor Mushroom Grown on Selenium-Rich Zeolitic Tuff as a Potential Novel Food Supplement" in Food Technology and Biotechnology, 60, no. 1 (2022):67-79,
https://doi.org/10.17113/ftb.60.01.22.7172 . .

TY  - JOUR
AU  - Marković, Sanja B.
AU  - Maciejewska, Natalia
AU  - Olszewski, Mateusz
AU  - Visnjevac, Aleksandar
AU  - Puerta, Adrian
AU  - Padron, Jose M.
AU  - Novaković, Irena
AU  - Kojić, Snežana
AU  - Fernandes, Henrique S.
AU  - Ramotowska, Sandra
AU  - Chylewska, Agnieszka
AU  - Makowski, Mariusz
AU  - Todorović, Tamara R.
AU  - Filipović, Nenad R.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1551
AB  - The biological activity of Cd compounds has been investigated scarce since Cd has been recognized as a human carcinogen. However, the toxicity of cadmium is comparable to the toxicity of noble metals such as Pt and Pd. The paradigm of metal toxicity has been challenged suggesting that metal toxicity is not a constant property, yet it depends on many factors like the presence of appropriate ligands. Studies on anticancer activity of cadmium complexes showed that the complexation of various ligands resulted in complexes that showed better activities than approved drugs. In the present study, cadmium complexes with biologically potent thiazolyl/selenazoyl-hydrazone ligands have been prepared, and tested for their activity against different types of tumor cell models. The complexation of ligands with Cd(II) resulted in a synergistic effect. The antiproliferative activity study revealed that all complexes are more active compared to 5-fluorouracil and cisplatin. The mechanism of tumor cell growth inhibition reveal that selenium-based compounds induce cell death in T-47D (gland carci-noma) cells through apoptosis via caspase-3/7 activation. Additionally, their pro-apoptotic effect was stronger compared to etoposide and cisplatin. Nuclease activity, detected by gel electrophoresis, may be the possible mechanism of anticancer action of investigated complexes.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters
VL  - 238
DO  - 10.1016/j.ejmech.2022.114449
ER  - 

@article{
author = "Marković, Sanja B. and Maciejewska, Natalia and Olszewski, Mateusz and Visnjevac, Aleksandar and Puerta, Adrian and Padron, Jose M. and Novaković, Irena and Kojić, Snežana and Fernandes, Henrique S. and Ramotowska, Sandra and Chylewska, Agnieszka and Makowski, Mariusz and Todorović, Tamara R. and Filipović, Nenad R.",
year = "2022",
abstract = "The biological activity of Cd compounds has been investigated scarce since Cd has been recognized as a human carcinogen. However, the toxicity of cadmium is comparable to the toxicity of noble metals such as Pt and Pd. The paradigm of metal toxicity has been challenged suggesting that metal toxicity is not a constant property, yet it depends on many factors like the presence of appropriate ligands. Studies on anticancer activity of cadmium complexes showed that the complexation of various ligands resulted in complexes that showed better activities than approved drugs. In the present study, cadmium complexes with biologically potent thiazolyl/selenazoyl-hydrazone ligands have been prepared, and tested for their activity against different types of tumor cell models. The complexation of ligands with Cd(II) resulted in a synergistic effect. The antiproliferative activity study revealed that all complexes are more active compared to 5-fluorouracil and cisplatin. The mechanism of tumor cell growth inhibition reveal that selenium-based compounds induce cell death in T-47D (gland carci-noma) cells through apoptosis via caspase-3/7 activation. Additionally, their pro-apoptotic effect was stronger compared to etoposide and cisplatin. Nuclease activity, detected by gel electrophoresis, may be the possible mechanism of anticancer action of investigated complexes.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters",
volume = "238",
doi = "10.1016/j.ejmech.2022.114449"
}

Marković, S. B., Maciejewska, N., Olszewski, M., Visnjevac, A., Puerta, A., Padron, J. M., Novaković, I., Kojić, S., Fernandes, H. S., Ramotowska, S., Chylewska, A., Makowski, M., Todorović, T. R.,& Filipović, N. R.. (2022). Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 238.
https://doi.org/10.1016/j.ejmech.2022.114449

Marković SB, Maciejewska N, Olszewski M, Visnjevac A, Puerta A, Padron JM, Novaković I, Kojić S, Fernandes HS, Ramotowska S, Chylewska A, Makowski M, Todorović TR, Filipović NR. Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters. in European Journal of Medicinal Chemistry. 2022;238.
doi:10.1016/j.ejmech.2022.114449 .

Marković, Sanja B., Maciejewska, Natalia, Olszewski, Mateusz, Visnjevac, Aleksandar, Puerta, Adrian, Padron, Jose M., Novaković, Irena, Kojić, Snežana, Fernandes, Henrique S., Ramotowska, Sandra, Chylewska, Agnieszka, Makowski, Mariusz, Todorović, Tamara R., Filipović, Nenad R., "Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters" in European Journal of Medicinal Chemistry, 238 (2022),
https://doi.org/10.1016/j.ejmech.2022.114449 . .

TY  - JOUR
AU  - Milinčić, Danijel
AU  - Stanisavljević, Nemanja
AU  - Kostić, Aleksandar
AU  - Gašić, Uroš
AU  - Stanojević, Slađana
AU  - Tešić, Živoslav
AU  - Pešić, Mirjana
PY  - 2022
UR  - https://www.mdpi.com/2076-3921/11/11/2164
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2064
AB  - This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.
T2  - Antioxidants
T2  - Antioxidants
T1  - Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion
IS  - 11
SP  - 2164
VL  - 11
DO  - 10.3390/antiox11112164
ER  - 

@article{
author = "Milinčić, Danijel and Stanisavljević, Nemanja and Kostić, Aleksandar and Gašić, Uroš and Stanojević, Slađana and Tešić, Živoslav and Pešić, Mirjana",
year = "2022",
abstract = "This study deals with the evaluation of the bioaccessibility and antioxidant properties of phenolic compounds from heat-treated skim goat-milk powder fortified with grape-pomace-seed extract, after in vitro gastrointestinal digestion. Ultra-high performance liquid chromatography coupled to diode array detection and mass spectrometry (UHPLC-DAD MS/MS) analysis confirmed the abundant presence of phenolic acids and flavan-3-ols in the grape-pomace-seed extract (SE) and heat-treated skim goat-milk/seed-extract powder (TME). After in vitro digestion of TME powder and recovery of total quantified phenolics, flavan-3-ols and phenolic acids were 18.11%, 24.54%, and 1.17%, respectively. Low recovery of grape-pomace-seed phenolics indicated strong milk protein–phenolic interactions. Electrophoretic analysis of a soluble fraction of digested heat-treated skim goat milk (TM) and TME samples showed the absence of bands originating from milk proteins, indicating their hydrolysis during in vitro gastrointestinal digestion. The digested TME sample had better antioxidant properties in comparison to the digested TM sample (except for the ferrous ion-chelating capacity, FCC), due to the presence of bioaccessible phenolics. Taking into account the contribution of the digestive cocktail, digested TME sample had lower values of total phenolic content (TPC), in vitro phosphomolybdenum reducing capacity (TAC) and ferric reducing power (FRP), compared to the undigested TME sample. These results could be attributed to low recovery of phenolic compounds. TME powder could be a good carrier of phenolics to the colon; thus, TME powder could be a promising ingredient in the formulation of functional food.",
journal = "Antioxidants, Antioxidants",
title = "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion",
number = "11",
pages = "2164",
volume = "11",
doi = "10.3390/antiox11112164"
}

Milinčić, D., Stanisavljević, N., Kostić, A., Gašić, U., Stanojević, S., Tešić, Ž.,& Pešić, M.. (2022). Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants, 11(11), 2164.
https://doi.org/10.3390/antiox11112164

Milinčić D, Stanisavljević N, Kostić A, Gašić U, Stanojević S, Tešić Ž, Pešić M. Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion. in Antioxidants. 2022;11(11):2164.
doi:10.3390/antiox11112164 .

Milinčić, Danijel, Stanisavljević, Nemanja, Kostić, Aleksandar, Gašić, Uroš, Stanojević, Slađana, Tešić, Živoslav, Pešić, Mirjana, "Bioaccessibility of Phenolic Compounds and Antioxidant Properties of Goat-Milk Powder Fortified with Grape-Pomace-Seed Extract after In Vitro Gastrointestinal Digestion" in Antioxidants, 11, no. 11 (2022):2164,
https://doi.org/10.3390/antiox11112164 . .

TY  - JOUR
AU  - Kostić, Aleksandar Z.
AU  - Milincić, Danijel D.
AU  - Stanisavljević, Nemanja
AU  - Gašić, Uroš
AU  - Lević, Steva
AU  - Kojić, Milan
AU  - Tesić, Zivoslav Lj.
AU  - Nedović, Viktor
AU  - Barac, Miroljub B.
AU  - Pesić, Mirjana B.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1416
AB  - The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermallytreated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7-76.2%) followed by phenolic acids (14.2-17.4%). Among individual compounds quercetin-3-O-glucoside (155.1-197.2 mu g/L) and p-coumaric acid (29.5-30.7 mu g/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS center dot(+)scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS center dot(+) radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.
PB  - Elsevier Sci Ltd, Oxford
T2  - Food Chemistry
T1  - Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen
VL  - 351
DO  - 10.1016/j.foodchem.2021.129310
ER  - 

@article{
author = "Kostić, Aleksandar Z. and Milincić, Danijel D. and Stanisavljević, Nemanja and Gašić, Uroš and Lević, Steva and Kojić, Milan and Tesić, Zivoslav Lj. and Nedović, Viktor and Barac, Miroljub B. and Pesić, Mirjana B.",
year = "2021",
abstract = "The aim of research was to determine polyphenols bioaccessibility and antioxidant properties of thermallytreated skimmed goat milk enriched with sunflower bee-collected pollen through in vitro digestion. HPLC analysis confirmed that pollen-enriched milk contained flavonols as the main phenolic fraction (80.7-76.2%) followed by phenolic acids (14.2-17.4%). Among individual compounds quercetin-3-O-glucoside (155.1-197.2 mu g/L) and p-coumaric acid (29.5-30.7 mu g/L) were the main quantified flavonols and phenolic acids, respectively. After digestion of milk/pollen sample, total polyphenols recovery was 30.71% with higher phenolic acids recovery (40.1%) compared to flavonols (28.3%) indicating strong interactions between caprine milk casein micelles and pollen polyphenols. Applied antioxidant assays (phosphomolybdenum, ABTS center dot(+)scavenging activity and ferrous-ion-chelating capacity) have confirmed complexity of prepared product- it had high ability to quench ABTS center dot(+) radicals and to form chelating complexes with Fe2+ ions. Digestion provoked 20% reduction in total antioxidant capacity compared to the initial sample. TTSG milk/pollen powder could be good functional ingredient.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Food Chemistry",
title = "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen",
volume = "351",
doi = "10.1016/j.foodchem.2021.129310"
}

Kostić, A. Z., Milincić, D. D., Stanisavljević, N., Gašić, U., Lević, S., Kojić, M., Tesić, Z. Lj., Nedović, V., Barac, M. B.,& Pesić, M. B.. (2021). Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry
Elsevier Sci Ltd, Oxford., 351.
https://doi.org/10.1016/j.foodchem.2021.129310

Kostić AZ, Milincić DD, Stanisavljević N, Gašić U, Lević S, Kojić M, Tesić ZL, Nedović V, Barac MB, Pesić MB. Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen. in Food Chemistry. 2021;351.
doi:10.1016/j.foodchem.2021.129310 .

Kostić, Aleksandar Z., Milincić, Danijel D., Stanisavljević, Nemanja, Gašić, Uroš, Lević, Steva, Kojić, Milan, Tesić, Zivoslav Lj., Nedović, Viktor, Barac, Miroljub B., Pesić, Mirjana B., "Polyphenol bioaccessibility and antioxidant properties of in vitro digested spray-dried thermally-treated skimmed goat milk enriched with pollen" in Food Chemistry, 351 (2021),
https://doi.org/10.1016/j.foodchem.2021.129310 . .

TY  - JOUR
AU  - Milincić, Danijel D.
AU  - Stanisavljević, Nemanja
AU  - Kostić, Aleksandar Z.
AU  - Soković Bajić, Svetlana
AU  - Kojić, Milan
AU  - Gašić, Uroš
AU  - Barac, Miroljub B.
AU  - Stanojević, Sladjana P.
AU  - Tesić, Zivoslav Lj.
AU  - Pesić, Mirjana B.
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1482
AB  - Phenolic compounds and biopotential (antioxidant, cellular antioxidant and cytotoxic activity) of grape pomace (GP) skin, seed, stem and whole GP originating from indigenous red grape variety Prokupac were evaluated. The UHPLC-Orbitrap MS4 analysis showed the presence of 75 phenolic compounds (57 non-anthocyanins and 18 anthocyanins), among which considerable content of ethyl gallate was observed and malvidin-3-O-hexoside-(8,8)-methylmethyne-(epi)catechin was identified. The stem had significant content of stilbenoids. The GP seed and the whole GP showed the highest antioxidant activity estimated by ABTS(center dot)+ DPPH center dot and H2O2 scavenging assays that were strongly correlated with the presence of flavan-3-ols, phenolic acids and ethyl gallate. The GP skin exerted cellular antioxidant activity on adenocarcinoma cells (EC50 = 56.4 mg TPCs/mL) which was strongly correlated with the presence of flavonols and anthocyanins. These by-products of Prokupac red grape variety are a notable source of phenolic compounds with good antioxidant activity that can be extensively used in food and pharmaceutical industry.
PB  - Elsevier, Amsterdam
T2  - Lwt-Food Science and Technology
T1  - Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety
VL  - 138
DO  - 10.1016/j.lwt.2020.110739
ER  - 

@article{
author = "Milincić, Danijel D. and Stanisavljević, Nemanja and Kostić, Aleksandar Z. and Soković Bajić, Svetlana and Kojić, Milan and Gašić, Uroš and Barac, Miroljub B. and Stanojević, Sladjana P. and Tesić, Zivoslav Lj. and Pesić, Mirjana B.",
year = "2021",
abstract = "Phenolic compounds and biopotential (antioxidant, cellular antioxidant and cytotoxic activity) of grape pomace (GP) skin, seed, stem and whole GP originating from indigenous red grape variety Prokupac were evaluated. The UHPLC-Orbitrap MS4 analysis showed the presence of 75 phenolic compounds (57 non-anthocyanins and 18 anthocyanins), among which considerable content of ethyl gallate was observed and malvidin-3-O-hexoside-(8,8)-methylmethyne-(epi)catechin was identified. The stem had significant content of stilbenoids. The GP seed and the whole GP showed the highest antioxidant activity estimated by ABTS(center dot)+ DPPH center dot and H2O2 scavenging assays that were strongly correlated with the presence of flavan-3-ols, phenolic acids and ethyl gallate. The GP skin exerted cellular antioxidant activity on adenocarcinoma cells (EC50 = 56.4 mg TPCs/mL) which was strongly correlated with the presence of flavonols and anthocyanins. These by-products of Prokupac red grape variety are a notable source of phenolic compounds with good antioxidant activity that can be extensively used in food and pharmaceutical industry.",
publisher = "Elsevier, Amsterdam",
journal = "Lwt-Food Science and Technology",
title = "Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety",
volume = "138",
doi = "10.1016/j.lwt.2020.110739"
}

Milincić, D. D., Stanisavljević, N., Kostić, A. Z., Soković Bajić, S., Kojić, M., Gašić, U., Barac, M. B., Stanojević, S. P., Tesić, Z. Lj.,& Pesić, M. B.. (2021). Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety. in Lwt-Food Science and Technology
Elsevier, Amsterdam., 138.
https://doi.org/10.1016/j.lwt.2020.110739

Milincić DD, Stanisavljević N, Kostić AZ, Soković Bajić S, Kojić M, Gašić U, Barac MB, Stanojević SP, Tesić ZL, Pesić MB. Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety. in Lwt-Food Science and Technology. 2021;138.
doi:10.1016/j.lwt.2020.110739 .

Milincić, Danijel D., Stanisavljević, Nemanja, Kostić, Aleksandar Z., Soković Bajić, Svetlana, Kojić, Milan, Gašić, Uroš, Barac, Miroljub B., Stanojević, Sladjana P., Tesić, Zivoslav Lj., Pesić, Mirjana B., "Phenolic compounds and biopotential of grape pomace extracts from Prokupac red grape variety" in Lwt-Food Science and Technology, 138 (2021),
https://doi.org/10.1016/j.lwt.2020.110739 . .

TY  - DATA
AU  - Kostić, Aleksandar
AU  - Milinčić, Danijel
AU  - Stanisavljević, Nemanja
AU  - Gašić, Uroš
AU  - Lević, Steva
AU  - Kojić, Milan
AU  - Tešić, Živoslav
AU  - Nedović, Viktor
AU  - Barać, Miroljub
AU  - Pešić, Mirjana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1607
PB  - Oxford : Elsevier Sci Ltd
T2  - Food Chemistry
T1  - Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Stanisavljević, N. S.; Gašić, U. M.; Lević, S.; Kojić, M. O.; Lj. Tešić, Ž.; Nedović, V.; Barać, M. B.; Pešić, M. B. Polyphenol Bioaccessibility and Antioxidant Properties of in Vitro Digested Spray-Dried Thermally-Treated Skimmed Goat Milk Enriched with Pollen. Food Chemistry 2021, 351. https://doi.org/10.1016/j.foodchem.2021.129310.
EP  - 2
SP  - 1
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1607
ER  - 

@misc{
author = "Kostić, Aleksandar and Milinčić, Danijel and Stanisavljević, Nemanja and Gašić, Uroš and Lević, Steva and Kojić, Milan and Tešić, Živoslav and Nedović, Viktor and Barać, Miroljub and Pešić, Mirjana",
year = "2021",
publisher = "Oxford : Elsevier Sci Ltd",
journal = "Food Chemistry",
title = "Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Stanisavljević, N. S.; Gašić, U. M.; Lević, S.; Kojić, M. O.; Lj. Tešić, Ž.; Nedović, V.; Barać, M. B.; Pešić, M. B. Polyphenol Bioaccessibility and Antioxidant Properties of in Vitro Digested Spray-Dried Thermally-Treated Skimmed Goat Milk Enriched with Pollen. Food Chemistry 2021, 351. https://doi.org/10.1016/j.foodchem.2021.129310.",
pages = "2-1",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1607"
}

Kostić, A., Milinčić, D., Stanisavljević, N., Gašić, U., Lević, S., Kojić, M., Tešić, Ž., Nedović, V., Barać, M.,& Pešić, M.. (2021). Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Stanisavljević, N. S.; Gašić, U. M.; Lević, S.; Kojić, M. O.; Lj. Tešić, Ž.; Nedović, V.; Barać, M. B.; Pešić, M. B. Polyphenol Bioaccessibility and Antioxidant Properties of in Vitro Digested Spray-Dried Thermally-Treated Skimmed Goat Milk Enriched with Pollen. Food Chemistry 2021, 351. https://doi.org/10.1016/j.foodchem.2021.129310.. in Food Chemistry
Oxford : Elsevier Sci Ltd., 1-2.
https://hdl.handle.net/21.15107/rcub_imagine_1607

Kostić A, Milinčić D, Stanisavljević N, Gašić U, Lević S, Kojić M, Tešić Ž, Nedović V, Barać M, Pešić M. Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Stanisavljević, N. S.; Gašić, U. M.; Lević, S.; Kojić, M. O.; Lj. Tešić, Ž.; Nedović, V.; Barać, M. B.; Pešić, M. B. Polyphenol Bioaccessibility and Antioxidant Properties of in Vitro Digested Spray-Dried Thermally-Treated Skimmed Goat Milk Enriched with Pollen. Food Chemistry 2021, 351. https://doi.org/10.1016/j.foodchem.2021.129310.. in Food Chemistry. 2021;:1-2.
https://hdl.handle.net/21.15107/rcub_imagine_1607 .

Kostić, Aleksandar, Milinčić, Danijel, Stanisavljević, Nemanja, Gašić, Uroš, Lević, Steva, Kojić, Milan, Tešić, Živoslav, Nedović, Viktor, Barać, Miroljub, Pešić, Mirjana, "Supplementary data for article: Kostić, A. Ž.; Milinčić, D. D.; Stanisavljević, N. S.; Gašić, U. M.; Lević, S.; Kojić, M. O.; Lj. Tešić, Ž.; Nedović, V.; Barać, M. B.; Pešić, M. B. Polyphenol Bioaccessibility and Antioxidant Properties of in Vitro Digested Spray-Dried Thermally-Treated Skimmed Goat Milk Enriched with Pollen. Food Chemistry 2021, 351. https://doi.org/10.1016/j.foodchem.2021.129310." in Food Chemistry (2021):1-2,
https://hdl.handle.net/21.15107/rcub_imagine_1607 .