TY  - JOUR
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1928
AB  - Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.
PB  - Springer Nature
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines
DO  - 10.1007/s00432-023-04930-9
ER  - 

@article{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
abstract = "Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.",
publisher = "Springer Nature",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines",
doi = "10.1007/s00432-023-04930-9"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology
Springer Nature..
https://doi.org/10.1007/s00432-023-04930-9

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. 2023;.
doi:10.1007/s00432-023-04930-9 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines" in Journal of Cancer Research and Clinical Oncology (2023),
https://doi.org/10.1007/s00432-023-04930-9 . .

TY  - JOUR
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1918
AB  - Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.
PB  - Springer Nature
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines
DO  - 10.1007/s00432-023-04930-9
ER  - 

@article{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
abstract = "Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.",
publisher = "Springer Nature",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines",
doi = "10.1007/s00432-023-04930-9"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology
Springer Nature..
https://doi.org/10.1007/s00432-023-04930-9

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. 2023;.
doi:10.1007/s00432-023-04930-9 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines" in Journal of Cancer Research and Clinical Oncology (2023),
https://doi.org/10.1007/s00432-023-04930-9 . .

TY  - DATA
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1929
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1929
ER  - 

@misc{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1929"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9. in Journal of Cancer Research and Clinical Oncology.
https://hdl.handle.net/21.15107/rcub_imagine_1929

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9. in Journal of Cancer Research and Clinical Oncology. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1929 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9" in Journal of Cancer Research and Clinical Oncology (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1929 .

TY  - JOUR
AU  - Kovač, Mirjana
AU  - Basarić, Dušica
AU  - Tomić, Branko
AU  - Gvozdenov, Maja
AU  - Backović, Dragana
AU  - Lalić-Ćosić, Sanja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1914
AB  - Direct oral anticoagulants (DOACs) administration significantly interferes with coagulation as-says. The aim of the study was to evaluate the effect of DOACs and DOAC-Remove® on coagulation assays dur-ing thrombophilia testing. Methods. The study was car-ried out from January 2019 to the end of June 2020. It in-cluded 30 DOAC-treated patients, 14 females and 16 males aged 23 to 63 (median age 47.6 years), tested for thrombophilia due to venous thromboembolism (VTE). Thrombophilia testing was performed using DOAC-Remove® tablets (activated charcoal). The results before and after DOAC-Remove® were compared. Results. Posi-tive lupus anticoagulant (LA) results were observed in 20% apixaban, 100% dabigatran, and 70% rivaroxaban-treated patients, while in samples after DOAC-Remove®, the LA positivity was observed only in one from the apix-aban group. Before DOAC-Remove®, the activated pro-tein C (APC) resistance (APC-R) was measurable in 40% dabigatran and 80% rivaroxaban-treated patients, while, after using DOAC-Remove®, the APC-R was measurable in all cases. Comparing the results obtained from the sam-ples before and after DOAC-Remove®, a difference was noted in relation to all dilute Russell's viper venom time (dRVVT) coagulation tests, except for the dRVVT ratio in the apixaban group. Clot-based methods for detecting the APC resistance were significantly affected by dabigatran and less by rivaroxaban. Conclusion. DOACs were prac-tically inactivated after the addition of the DOAC-Remove®, which made it possible to perform analyses for the LA and APC-R testing freely and obtain relevant re-sults.
AB  - Primena direktnih oralnih antikoagulansa (DOAK)značajno utiče na testove koagulacije. Cilj rada bio je da se pro-ceni uticaj DOAK i DOAC-Remove® tableta (aktivni ugalj) natestove koagulacije tokom ispitivanja trombofilije. Metode.Istraživanjem, sprovedenim od januara 2019. do juna 2020.godine, obuhvaćeno je 30 bolesnika lečenih DOAK-om itestiranih na trombofiliju zbog venskog tromboembolzma(VTE). Bilo je 14 žena i 16 muškaraca, starosti od 23 do 63godine (medijana 47,6 godina). Ispitivanje trombofilije izvršenoje upotrebom DOAC-Remove® tableta (aktivni ugalj).Upoređivani su rezultati pre i posle primene DOAC-Remove®.Rezultati. Pozitivni rezultati za lupus antikoagulantni (LA) testdobijeni su kod 20% bolesnika lečenih apiksabanom, kod100% bolesnika lečenih dabigatranom i kod 70% lečenih riva-roksabanom, a u uzorcima posle DOAC-Remove® pozitivnostna LA dobijena je samo kod jednog bolesnika iz grupe lečnihapiksabanom. Pre primene DOAC-Remove®, rezistencija naaktivisani protein C (activated protein C resistance – APC-R) bila jemerljiva kod 40% i 80% bolesnika lečenih dabigatranom, od-nosno rivaroksabanom, dok je posle primene DOAC-Remove®, APC-R bila merljiva u svim slučajevima.Upoređivanjem rezultata dobijenih iz uzoraka pre i posleprimene DOAC-Remove®, primećena je razlika u odnosu nasve testove vremena koagulacije izvršene razblaženim Russell-ovim zmijskim otrovom (dilute Russell’s viper venom time –dRVVT), osim dRVVT u grupi bolesnika lečenih apiksabanom.Na koagulacionu metodu za otkrivanje APC-R značajno je uti-cao dabigatran, a manje rivaroksaban. Zaključak. Nakonprimene DOAC-Remove® tableta, DOAK su praktičnoinaktivisani što je omogućilo izvođenje analiza za LA i APC-R idobijanje relevantnih rezultata testova.
PB  - Inst. Sci. inf., Univ. Defence in Belgrade
T2  - Vojnosanitetski Pregled
T1  - Influence of DOACS and DOAC-REMOVE® on coagulation assays during thrombophilia testing in DOAC-treated patients
T1  - Uticaj DOAK i DOAC-REMOVE® na testove koagulacije u toku testiranjatrombofilije kod bolesnika lečenih primenom DOAK
EP  - 1254
IS  - 12
SP  - 1248
VL  - 79
DO  - 10.2298/VSP210217101K
ER  - 

@article{
author = "Kovač, Mirjana and Basarić, Dušica and Tomić, Branko and Gvozdenov, Maja and Backović, Dragana and Lalić-Ćosić, Sanja",
year = "2022",
abstract = "Direct oral anticoagulants (DOACs) administration significantly interferes with coagulation as-says. The aim of the study was to evaluate the effect of DOACs and DOAC-Remove® on coagulation assays dur-ing thrombophilia testing. Methods. The study was car-ried out from January 2019 to the end of June 2020. It in-cluded 30 DOAC-treated patients, 14 females and 16 males aged 23 to 63 (median age 47.6 years), tested for thrombophilia due to venous thromboembolism (VTE). Thrombophilia testing was performed using DOAC-Remove® tablets (activated charcoal). The results before and after DOAC-Remove® were compared. Results. Posi-tive lupus anticoagulant (LA) results were observed in 20% apixaban, 100% dabigatran, and 70% rivaroxaban-treated patients, while in samples after DOAC-Remove®, the LA positivity was observed only in one from the apix-aban group. Before DOAC-Remove®, the activated pro-tein C (APC) resistance (APC-R) was measurable in 40% dabigatran and 80% rivaroxaban-treated patients, while, after using DOAC-Remove®, the APC-R was measurable in all cases. Comparing the results obtained from the sam-ples before and after DOAC-Remove®, a difference was noted in relation to all dilute Russell's viper venom time (dRVVT) coagulation tests, except for the dRVVT ratio in the apixaban group. Clot-based methods for detecting the APC resistance were significantly affected by dabigatran and less by rivaroxaban. Conclusion. DOACs were prac-tically inactivated after the addition of the DOAC-Remove®, which made it possible to perform analyses for the LA and APC-R testing freely and obtain relevant re-sults., Primena direktnih oralnih antikoagulansa (DOAK)značajno utiče na testove koagulacije. Cilj rada bio je da se pro-ceni uticaj DOAK i DOAC-Remove® tableta (aktivni ugalj) natestove koagulacije tokom ispitivanja trombofilije. Metode.Istraživanjem, sprovedenim od januara 2019. do juna 2020.godine, obuhvaćeno je 30 bolesnika lečenih DOAK-om itestiranih na trombofiliju zbog venskog tromboembolzma(VTE). Bilo je 14 žena i 16 muškaraca, starosti od 23 do 63godine (medijana 47,6 godina). Ispitivanje trombofilije izvršenoje upotrebom DOAC-Remove® tableta (aktivni ugalj).Upoređivani su rezultati pre i posle primene DOAC-Remove®.Rezultati. Pozitivni rezultati za lupus antikoagulantni (LA) testdobijeni su kod 20% bolesnika lečenih apiksabanom, kod100% bolesnika lečenih dabigatranom i kod 70% lečenih riva-roksabanom, a u uzorcima posle DOAC-Remove® pozitivnostna LA dobijena je samo kod jednog bolesnika iz grupe lečnihapiksabanom. Pre primene DOAC-Remove®, rezistencija naaktivisani protein C (activated protein C resistance – APC-R) bila jemerljiva kod 40% i 80% bolesnika lečenih dabigatranom, od-nosno rivaroksabanom, dok je posle primene DOAC-Remove®, APC-R bila merljiva u svim slučajevima.Upoređivanjem rezultata dobijenih iz uzoraka pre i posleprimene DOAC-Remove®, primećena je razlika u odnosu nasve testove vremena koagulacije izvršene razblaženim Russell-ovim zmijskim otrovom (dilute Russell’s viper venom time –dRVVT), osim dRVVT u grupi bolesnika lečenih apiksabanom.Na koagulacionu metodu za otkrivanje APC-R značajno je uti-cao dabigatran, a manje rivaroksaban. Zaključak. Nakonprimene DOAC-Remove® tableta, DOAK su praktičnoinaktivisani što je omogućilo izvođenje analiza za LA i APC-R idobijanje relevantnih rezultata testova.",
publisher = "Inst. Sci. inf., Univ. Defence in Belgrade",
journal = "Vojnosanitetski Pregled",
title = "Influence of DOACS and DOAC-REMOVE® on coagulation assays during thrombophilia testing in DOAC-treated patients, Uticaj DOAK i DOAC-REMOVE® na testove koagulacije u toku testiranjatrombofilije kod bolesnika lečenih primenom DOAK",
pages = "1254-1248",
number = "12",
volume = "79",
doi = "10.2298/VSP210217101K"
}

Kovač, M., Basarić, D., Tomić, B., Gvozdenov, M., Backović, D.,& Lalić-Ćosić, S.. (2022). Influence of DOACS and DOAC-REMOVE® on coagulation assays during thrombophilia testing in DOAC-treated patients. in Vojnosanitetski Pregled
Inst. Sci. inf., Univ. Defence in Belgrade., 79(12), 1248-1254.
https://doi.org/10.2298/VSP210217101K

Kovač M, Basarić D, Tomić B, Gvozdenov M, Backović D, Lalić-Ćosić S. Influence of DOACS and DOAC-REMOVE® on coagulation assays during thrombophilia testing in DOAC-treated patients. in Vojnosanitetski Pregled. 2022;79(12):1248-1254.
doi:10.2298/VSP210217101K .

Kovač, Mirjana, Basarić, Dušica, Tomić, Branko, Gvozdenov, Maja, Backović, Dragana, Lalić-Ćosić, Sanja, "Influence of DOACS and DOAC-REMOVE® on coagulation assays during thrombophilia testing in DOAC-treated patients" in Vojnosanitetski Pregled, 79, no. 12 (2022):1248-1254,
https://doi.org/10.2298/VSP210217101K . .

TY  - JOUR
AU  - Rakićević, Ljiljana
AU  - Kovač, Mirjana
AU  - Radojković, Dragica
AU  - Radojković, Milica
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1587
AB  - Uvod/Cilj Terapija kumarinima predstavlja jedan od najboljih modela za primenu farmakogenetike. Doprinos faktora koji utiču na terapiju kumarinima može značajno da varira između etničkih grupa, što opravdava sprovođenje studija specifičnih za populaciju. Cilj ove studije je bio da se analizira uticaj najvažnijih genetičkih faktora (geni VKORC1 i CYP2C9) koji utiču na terapiju kumarinima kod bolesnika iz Srbije. Metode Sprovedena je retrospektivna studija koja je obuhvatila 207 bolesnika na terapiji acenokumarolom. Genetičke analize su vršene direktnim sekvenciranjem. Analiziran je uticaj na dozu acenokumarola varijanti (VKORC1*2, CYP2C9*2, CYP2C9*3) koje izazivaju preosetljivost i varijanti gena VKORC1 koje izazivaju rezistenciju na kumarine. Višestruka regresiona analiza je korišćena u cilju dizajniranja matematičkog modela za predviđanje individualne doze leka na osnovu kliničko-demografskih i genetičkih podataka. Rezultati Studija je potvrdila značajan uticaj analiziranih genetičkih faktora na održavanje doze acenokumarola. Dizajniran je matematički model za predviđanje individualne doze acenokumarola i njegov nekorigovani R2 je bio 61,8. Prilikom testiranja, naš model je dao R2 vrednost od 42,6 i pokazao bolje predviđanje u poređenju sa modelom koji su dali drugi autori. Kod analiziranih bolesnika pronađeno je devet različitih varijanti u kodirajućem regionu gena VKORC1. Među nosiocima ovih varijanti 78% je bilo potpuno rezistentno, te nije bilo moguće postići terapeutski efekat čak ni sa visokim dozama acenokumarola. Zaključci Populacioni model za predviđanje individualne doze acenokumarola može pokazati prednosti u odnosu na modele koji se koriste na generalizovan način. Takođe, VKORC1 varijante koje izazivaju rezistenciju na kumarin treba uzeti u obzir prilikom planiranja terapije.
AB  - Introduction/Objective Coumarin therapy represents one of the best models for applying pharmacogenetics. The contribution of factors influencing coumarin therapy can vary significantly between ethnic groups, which justifies conducting population-specific studies. The aim of this study was to analyze the influence of the most important genetic factors (VKORC1 and CYP2C9 genes) that affect coumarin therapy in patients from Serbia. Methods A retrospective study involving 207 patients on acenocoumarol therapy was conducted. Genetic analyses were performed by direct sequencing. Influence on acenocoumarol dose of variants (VKORC1, CYP2C9*2, CYP2C9*3) causing hypersensitivity and VKORC1 variants causing resistance to acenocoumarol were analyzed. Multiple regression analysis was used to design a mathematical model for predicting individual drug dosage based on clinical-demographic and genetic data. Results The study confirmed significant influence of the analyzed genetic factors on acenocoumarol maintenance dose. We designed mathematical model for predicting individual acenocoumarol dose and its unadjusted R2 was 61.8. In the testing cohort, our model gave R2 value of 42.6 and showed better prediction in comparison with model given by other authors. In the analyzed patients, nine different variants in the VKORC1 coding region were found. Among carriers of these variants 78% were completely resistant, and it was not possible to achieve therapeutic effect even with high doses of acenocoumarol. Conclusions Population-specific model for prediction individual dose of acenocoumarol, may show advantages over protocols that are used in a generalized manner. Also, VKORC1 variants which cause coumarin resistance should be considered when planning therapy.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Varijante gena "VKORC1" i "CYP2C9" kao farmakogenetički faktori u terapiji acenokumarolom kod bolesnika u Srbiji - razmatranje preosetljivosti i rezistencije
T1  - The 'VKORC1' and 'CYP2C9' gene variants as pharmacogenetic factors in acenocoumarol therapy in Serbian patients: Consideration of hypersensitivity and resistance
EP  - 162
IS  - 3-4
SP  - 156
VL  - 150
DO  - 10.2298/SARH211118013R
ER  - 

@article{
author = "Rakićević, Ljiljana and Kovač, Mirjana and Radojković, Dragica and Radojković, Milica",
year = "2022",
abstract = "Uvod/Cilj Terapija kumarinima predstavlja jedan od najboljih modela za primenu farmakogenetike. Doprinos faktora koji utiču na terapiju kumarinima može značajno da varira između etničkih grupa, što opravdava sprovođenje studija specifičnih za populaciju. Cilj ove studije je bio da se analizira uticaj najvažnijih genetičkih faktora (geni VKORC1 i CYP2C9) koji utiču na terapiju kumarinima kod bolesnika iz Srbije. Metode Sprovedena je retrospektivna studija koja je obuhvatila 207 bolesnika na terapiji acenokumarolom. Genetičke analize su vršene direktnim sekvenciranjem. Analiziran je uticaj na dozu acenokumarola varijanti (VKORC1*2, CYP2C9*2, CYP2C9*3) koje izazivaju preosetljivost i varijanti gena VKORC1 koje izazivaju rezistenciju na kumarine. Višestruka regresiona analiza je korišćena u cilju dizajniranja matematičkog modela za predviđanje individualne doze leka na osnovu kliničko-demografskih i genetičkih podataka. Rezultati Studija je potvrdila značajan uticaj analiziranih genetičkih faktora na održavanje doze acenokumarola. Dizajniran je matematički model za predviđanje individualne doze acenokumarola i njegov nekorigovani R2 je bio 61,8. Prilikom testiranja, naš model je dao R2 vrednost od 42,6 i pokazao bolje predviđanje u poređenju sa modelom koji su dali drugi autori. Kod analiziranih bolesnika pronađeno je devet različitih varijanti u kodirajućem regionu gena VKORC1. Među nosiocima ovih varijanti 78% je bilo potpuno rezistentno, te nije bilo moguće postići terapeutski efekat čak ni sa visokim dozama acenokumarola. Zaključci Populacioni model za predviđanje individualne doze acenokumarola može pokazati prednosti u odnosu na modele koji se koriste na generalizovan način. Takođe, VKORC1 varijante koje izazivaju rezistenciju na kumarin treba uzeti u obzir prilikom planiranja terapije., Introduction/Objective Coumarin therapy represents one of the best models for applying pharmacogenetics. The contribution of factors influencing coumarin therapy can vary significantly between ethnic groups, which justifies conducting population-specific studies. The aim of this study was to analyze the influence of the most important genetic factors (VKORC1 and CYP2C9 genes) that affect coumarin therapy in patients from Serbia. Methods A retrospective study involving 207 patients on acenocoumarol therapy was conducted. Genetic analyses were performed by direct sequencing. Influence on acenocoumarol dose of variants (VKORC1, CYP2C9*2, CYP2C9*3) causing hypersensitivity and VKORC1 variants causing resistance to acenocoumarol were analyzed. Multiple regression analysis was used to design a mathematical model for predicting individual drug dosage based on clinical-demographic and genetic data. Results The study confirmed significant influence of the analyzed genetic factors on acenocoumarol maintenance dose. We designed mathematical model for predicting individual acenocoumarol dose and its unadjusted R2 was 61.8. In the testing cohort, our model gave R2 value of 42.6 and showed better prediction in comparison with model given by other authors. In the analyzed patients, nine different variants in the VKORC1 coding region were found. Among carriers of these variants 78% were completely resistant, and it was not possible to achieve therapeutic effect even with high doses of acenocoumarol. Conclusions Population-specific model for prediction individual dose of acenocoumarol, may show advantages over protocols that are used in a generalized manner. Also, VKORC1 variants which cause coumarin resistance should be considered when planning therapy.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Varijante gena "VKORC1" i "CYP2C9" kao farmakogenetički faktori u terapiji acenokumarolom kod bolesnika u Srbiji - razmatranje preosetljivosti i rezistencije, The 'VKORC1' and 'CYP2C9' gene variants as pharmacogenetic factors in acenocoumarol therapy in Serbian patients: Consideration of hypersensitivity and resistance",
pages = "162-156",
number = "3-4",
volume = "150",
doi = "10.2298/SARH211118013R"
}

Rakićević, L., Kovač, M., Radojković, D.,& Radojković, M.. (2022). Varijante gena "VKORC1" i "CYP2C9" kao farmakogenetički faktori u terapiji acenokumarolom kod bolesnika u Srbiji - razmatranje preosetljivosti i rezistencije. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 150(3-4), 156-162.
https://doi.org/10.2298/SARH211118013R

Rakićević L, Kovač M, Radojković D, Radojković M. Varijante gena "VKORC1" i "CYP2C9" kao farmakogenetički faktori u terapiji acenokumarolom kod bolesnika u Srbiji - razmatranje preosetljivosti i rezistencije. in Srpski arhiv za celokupno lekarstvo. 2022;150(3-4):156-162.
doi:10.2298/SARH211118013R .

Rakićević, Ljiljana, Kovač, Mirjana, Radojković, Dragica, Radojković, Milica, "Varijante gena "VKORC1" i "CYP2C9" kao farmakogenetički faktori u terapiji acenokumarolom kod bolesnika u Srbiji - razmatranje preosetljivosti i rezistencije" in Srpski arhiv za celokupno lekarstvo, 150, no. 3-4 (2022):156-162,
https://doi.org/10.2298/SARH211118013R . .

TY  - THES
AU  - Bošković, Srđan
PY  - 2021
UR  - https://eteze.bg.ac.rs/application/showtheses?thesesId=8570
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:25620/bdef:Content/download
UR  - https://nardus.mpn.gov.rs/handle/123456789/19043
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/65
AB  - U popriječno-prugastim mišićima članovi familije mišićnih proteina sa ankirinskim ponovcima (eng. Muscle Ankyrin Repeat Proteins, MARP) učestvuju u mehanotransdukciji signala sa sarkomere u jedro, gdje modulišu ekspresiju ciljnih gena. Eksprimirani su tokom razvića srca i skeletnih mišića, povećanog mehaničkog opterećenja ovih organa i u patološkim stanjima poput infarkta miokarda i miopatija. Smatra se da ovi proteini imaju ulogu u adaptaciji mišićne ćelije na stres, ali je ona i dalje nedovoljno istražena. Kako bi upotpunili znanje o MARP familiji, njeni članovi proučavani su u zebrici, model organizmu naročito pogodnom za in vivo analizu razvića, genetičke manipulacije i proučavanje mehanizama regeneracije. Ekspresija ortologa MARP gena zebrice (ankrd1a, ankrd1b i ankrd2) analizirana je tokom razvića i u odgovoru na dvije vrste stresa: povećanu fizičku aktivnost i povredu srca. Generisanje mutanata za MARP gene omogućilo je njihovu funkcionalnu analizu u ovim procesima. Određen je profil ekspresije MARP gena tokom razvića zebrice, od embriona do adulta, a reporterska linija TgBAC(ankrd1a:EGFP) pružila je uvid u ekspresiju ankrd1a sa visokom rezolucijom. Povećana fizička aktivnost indukovala je ekspresiju MARP gena u srcu i skeletnim mišićima zebrice. Ekspresija ankrd1a je povećana u kardiomiocitima graničnog regiona povrede i zavisna od stadijuma remodelovanja miokarda u regenerišućem srcu. Iako mutacije u MARP genima nisu dovele do očigledne promjene fenotipa, kod adultnih jedinki zebrice genotipa ankrd1a-/- primjećena je smanjena proliferacija kardiomiocita nakon povrede srca, ali bez uticaja na konačni ishod regeneracije. Ovim istraživanjem napravljen je ključni korak ka uspostavljanju zebrice kao model ogranizma za proučavanje funkcije članova MARP familije.
AB  - Members of the muscle ankyrin repeat protein (MARP) family in striated muscles play a role in mechanotransduction of signals from the sarcomere to the nucleus, where they modulate expression of target genes. MARPs are expressed during heart and skeletal muscle development, increased mechanical load and in pathological conditions such as myocardial infarction and myopathies. Their presumed role in muscle adaptation to stress remains poorly understood. To extend our knowledge of the MARP family members, they were studied in the zebrafish, a model organism well suited for developmental and regeneration studies, as well as genetic manipulations. Expression of zebrafish MARP gene orthologues (ankrd1a, ankrd1b, ankrd2) was analysed during development and in response to two types of stress: increased physical activity and cardiac injury. Introduction of mutations in the MARP family genes allowed for functional analysis of its members in these processes. Expression profile of MARP genes during zebrafish development was determined in stages from embryo to adult, while the transgenic reporter line TgBAC(ankrd1a:EGFP) provided tracking of ankrd1a expression with higher resolution. Increased physical activity induced the expression of MARP genes in zebrafish heart and skeletal muscles. Expression of ankrd1a was found increased in the injury border zone cardiomyocytes and dependent on myocardium remodelling stage in the regenerating heart. Although mutations in MARP genes did not cause phenotype alterations, a lower rate of cardiomyocytes proliferation was observed in ankrd1a-/- zebrafish following cardiac injury, but with no effect on the regeneration outcome. This study is a key step towards establishing the zebrafish as a model organism for functional studies of the MARP family.
PB  - Univerzitet u Beogradu, Biološki fakultet
T1  - Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres
T1  - Characterization of zebrafish ankrd1a, ankrd1b and ankrd2 genes in development and stress response
UR  - https://hdl.handle.net/21.15107/rcub_nardus_19043
ER  - 

@phdthesis{
author = "Bošković, Srđan",
year = "2021",
abstract = "U popriječno-prugastim mišićima članovi familije mišićnih proteina sa ankirinskim ponovcima (eng. Muscle Ankyrin Repeat Proteins, MARP) učestvuju u mehanotransdukciji signala sa sarkomere u jedro, gdje modulišu ekspresiju ciljnih gena. Eksprimirani su tokom razvića srca i skeletnih mišića, povećanog mehaničkog opterećenja ovih organa i u patološkim stanjima poput infarkta miokarda i miopatija. Smatra se da ovi proteini imaju ulogu u adaptaciji mišićne ćelije na stres, ali je ona i dalje nedovoljno istražena. Kako bi upotpunili znanje o MARP familiji, njeni članovi proučavani su u zebrici, model organizmu naročito pogodnom za in vivo analizu razvića, genetičke manipulacije i proučavanje mehanizama regeneracije. Ekspresija ortologa MARP gena zebrice (ankrd1a, ankrd1b i ankrd2) analizirana je tokom razvića i u odgovoru na dvije vrste stresa: povećanu fizičku aktivnost i povredu srca. Generisanje mutanata za MARP gene omogućilo je njihovu funkcionalnu analizu u ovim procesima. Određen je profil ekspresije MARP gena tokom razvića zebrice, od embriona do adulta, a reporterska linija TgBAC(ankrd1a:EGFP) pružila je uvid u ekspresiju ankrd1a sa visokom rezolucijom. Povećana fizička aktivnost indukovala je ekspresiju MARP gena u srcu i skeletnim mišićima zebrice. Ekspresija ankrd1a je povećana u kardiomiocitima graničnog regiona povrede i zavisna od stadijuma remodelovanja miokarda u regenerišućem srcu. Iako mutacije u MARP genima nisu dovele do očigledne promjene fenotipa, kod adultnih jedinki zebrice genotipa ankrd1a-/- primjećena je smanjena proliferacija kardiomiocita nakon povrede srca, ali bez uticaja na konačni ishod regeneracije. Ovim istraživanjem napravljen je ključni korak ka uspostavljanju zebrice kao model ogranizma za proučavanje funkcije članova MARP familije., Members of the muscle ankyrin repeat protein (MARP) family in striated muscles play a role in mechanotransduction of signals from the sarcomere to the nucleus, where they modulate expression of target genes. MARPs are expressed during heart and skeletal muscle development, increased mechanical load and in pathological conditions such as myocardial infarction and myopathies. Their presumed role in muscle adaptation to stress remains poorly understood. To extend our knowledge of the MARP family members, they were studied in the zebrafish, a model organism well suited for developmental and regeneration studies, as well as genetic manipulations. Expression of zebrafish MARP gene orthologues (ankrd1a, ankrd1b, ankrd2) was analysed during development and in response to two types of stress: increased physical activity and cardiac injury. Introduction of mutations in the MARP family genes allowed for functional analysis of its members in these processes. Expression profile of MARP genes during zebrafish development was determined in stages from embryo to adult, while the transgenic reporter line TgBAC(ankrd1a:EGFP) provided tracking of ankrd1a expression with higher resolution. Increased physical activity induced the expression of MARP genes in zebrafish heart and skeletal muscles. Expression of ankrd1a was found increased in the injury border zone cardiomyocytes and dependent on myocardium remodelling stage in the regenerating heart. Although mutations in MARP genes did not cause phenotype alterations, a lower rate of cardiomyocytes proliferation was observed in ankrd1a-/- zebrafish following cardiac injury, but with no effect on the regeneration outcome. This study is a key step towards establishing the zebrafish as a model organism for functional studies of the MARP family.",
publisher = "Univerzitet u Beogradu, Biološki fakultet",
title = "Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres, Characterization of zebrafish ankrd1a, ankrd1b and ankrd2 genes in development and stress response",
url = "https://hdl.handle.net/21.15107/rcub_nardus_19043"
}

Bošković, S.. (2021). Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres. 
Univerzitet u Beogradu, Biološki fakultet..
https://hdl.handle.net/21.15107/rcub_nardus_19043

Bošković S. Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_19043 .

Bošković, Srđan, "Karakterizacija gena ankrd1a, ankrd1b i ankrd2 zebrice (Danio rerio) u razviću i odgovoru na stres" (2021),
https://hdl.handle.net/21.15107/rcub_nardus_19043 .

TY  - JOUR
AU  - Prosenc-Zmrzljak, Ursula
AU  - Kosir, Rok
AU  - Krivokapić, Zoran
AU  - Radojković, Dragica
AU  - Nikolić, Aleksandra
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1494
AB  - Liquid biopsy and cell-free DNA (cfDNA) show great promise in cancer diagnostics. In this study, we designed a custom droplet digital PCR (ddPCR) assay for the quantification and quality control of cfDNA isolated from serum. The assay was validated on a group of locally advanced colorectal cancer (CRC) patients and two control groups-patients with hemorrhoids and healthy individuals. The assay shows a high correlation with Qubit measurement (r = 0.976) but offers a higher dynamic range. Mean concentrations of cfDNA were 12.36 ng/mu L, 5.17 ng/mu L, and 0.29 ng/mu L for CRC, hemorrhoid patients, and healthy controls, respectively. The quality of cfDNA was assessed with the measurement of B-cell DNA contamination. On a subset of CRC patients, we compared the mutation status on KRAS (G12A, G12D, G12V, G13D) and BRAF (V600E) genes in the primary tumor and cfDNA isolated from the serum. A total of 70.6% of primary tumor samples were mutated, and the mean fractional abundance of mutations was 9.50%. The matching serum samples were mutated in 38% cases with an average fractional abundance of 0.23%. We conclude that any decisions based solely on the amount of cfDNA present in patient serum must be interpreted carefully and in the context of co-morbidities. This study explores the potential of ddPCR somatic mutations detection from liquid biopsy as a supplement to tissue biopsy in targeted personalized CRC patient management.
PB  - MDPI, Basel
T2  - Genes
T1  - Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients
IS  - 2
VL  - 12
DO  - 10.3390/genes12020289
ER  - 

@article{
author = "Prosenc-Zmrzljak, Ursula and Kosir, Rok and Krivokapić, Zoran and Radojković, Dragica and Nikolić, Aleksandra",
year = "2021",
abstract = "Liquid biopsy and cell-free DNA (cfDNA) show great promise in cancer diagnostics. In this study, we designed a custom droplet digital PCR (ddPCR) assay for the quantification and quality control of cfDNA isolated from serum. The assay was validated on a group of locally advanced colorectal cancer (CRC) patients and two control groups-patients with hemorrhoids and healthy individuals. The assay shows a high correlation with Qubit measurement (r = 0.976) but offers a higher dynamic range. Mean concentrations of cfDNA were 12.36 ng/mu L, 5.17 ng/mu L, and 0.29 ng/mu L for CRC, hemorrhoid patients, and healthy controls, respectively. The quality of cfDNA was assessed with the measurement of B-cell DNA contamination. On a subset of CRC patients, we compared the mutation status on KRAS (G12A, G12D, G12V, G13D) and BRAF (V600E) genes in the primary tumor and cfDNA isolated from the serum. A total of 70.6% of primary tumor samples were mutated, and the mean fractional abundance of mutations was 9.50%. The matching serum samples were mutated in 38% cases with an average fractional abundance of 0.23%. We conclude that any decisions based solely on the amount of cfDNA present in patient serum must be interpreted carefully and in the context of co-morbidities. This study explores the potential of ddPCR somatic mutations detection from liquid biopsy as a supplement to tissue biopsy in targeted personalized CRC patient management.",
publisher = "MDPI, Basel",
journal = "Genes",
title = "Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients",
number = "2",
volume = "12",
doi = "10.3390/genes12020289"
}

Prosenc-Zmrzljak, U., Kosir, R., Krivokapić, Z., Radojković, D.,& Nikolić, A.. (2021). Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients. in Genes
MDPI, Basel., 12(2).
https://doi.org/10.3390/genes12020289

Prosenc-Zmrzljak U, Kosir R, Krivokapić Z, Radojković D, Nikolić A. Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients. in Genes. 2021;12(2).
doi:10.3390/genes12020289 .

Prosenc-Zmrzljak, Ursula, Kosir, Rok, Krivokapić, Zoran, Radojković, Dragica, Nikolić, Aleksandra, "Detection of Somatic Mutations with ddPCR from Liquid Biopsy of Colorectal Cancer Patients" in Genes, 12, no. 2 (2021),
https://doi.org/10.3390/genes12020289 . .

TY  - JOUR
AU  - Kovač, Mirjana
AU  - Erić, Bojana
AU  - Stojneva-Istatkov, Jelena
AU  - Lukić, Vojislav
AU  - Milić, Ana
AU  - Vukičević, Dragana
AU  - Orlić, Dušan
AU  - Tomić, Branko
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1412
AB  - Uvod/Cilj. Određivanje nivoa hemoglobin (Hb) je rutinski deo selekcije dobrovoljnih davalaca krvi. Prethodno publikovane studije pokazale su da se nedostatak gvožđa javlja kod redovnih davalaca krvi. Cilj ove prospektivne studije je bio da se utvrdi status gvožđa kod davalaca kod kojih je pre davanja utvrđen nizak nivo Hb i da se proceni stepen korelacije kapilarnih metoda, sa referentnom metodom određivanja Hb iz venske krvi, kao i sa i nivoom feritina. Metode. U periodu od februara 2017. do decembra 2018. godine, bilo je uključeno 200 dobrovoljnih davalaca sa niskim nivoima Hb, starosne dobi 19-64 godine (medijana 39 godina). Hb je određivan primenom metode bakar sulfat, kapilarnom metodom "HemoCue", i iz venske krvi u sklopu određivanja kompletne krvne slike (KKS). Nivo feritina određivan je primenom turbodimetrijske metode. Rezultati. Nizak nivo feritina utvrđen je kod 42,7% muškaraca i 57,3% žena (p = 0,008). U odnosu na nivo feritina  lt  12 mg/L, odnosno  gt  50 mg/L, zabeležena je značajna razlika između polova (p = 0,023, odnosno p = 0,022). Poređenje vrednosti Hb dobijenih kapilarnim metodama u odnosu na referentne vrednosti Hb, određene iz KKS, pokazalo je da metoda s bakar sulfatom daje lažno niske vrednosti Hb kod 10,5% slučajeva (p  lt  0,001). Vrednosti Hb dobijene metodom "HemoCue-a" značajno su korelirale sa vrednostima Hb iz KKS, dok korelacija između nivoa feritina i Hb, određenog pomoću obe kapilarne metode, nije uočena. Zaključak. Kod 51,5% naših davalaca krvi koji su vraćeni zbog niskih vrednosti Hb utvrđen je snižen nivo feritina. Na osnovu ovih rezultata neophodno je odrediti algoritam za detekciju nedostatka gvožđa, dok je kapilarna metoda (HemoCue) pogodnija metoda za testiranje Hb pre davanja krvi.
AB  - Background/Aim. Haemoglobin (Hb) determination is a routine part of the blood donor selection process. Previously reported studies have revealed that iron deficiency is common in frequent donors. This prospective investigation was aimed at examining iron status among blood donors with low circulating Hb and evaluating correlation between Hb values determined by capillary methods and those obtained by reference method from venous blood count (BC), as well as ferritin level. Methods. Between February 2017 and December 2018, 200 consecutively recruited regular blood donors with low Hb, aged 19 to 64 years (median 39), were included. Hb level was determined using the copper sulphate method, the HemoCue capillary method, and also from venous blood within the complete blood count (CBC) test. Plasma ferritin was determined turbidimetrically. Results. In 42.7% of men and 57.3% of women, ferritin concentration was low (p = 0.008). The relative numbers of males and females, with levels  lt  12 mg/L (p = 0.023) or  gt  50 mg/L (p = 0.022), differed. Comparison of the values obtained with the capillary methods with reference Hb levels obtained from the CBC test showed that the copper sulphate procedure gave false fails in 10.5% of cases (p  lt  0.001). Hb values from HemoCue were significantly correlated with Hb values from the CBC test, but no correlation was observed between ferritin levels and Hb levels determined by both capillary method. Conclusion. Low ferritin was observed in 51.5% of Serbian blood donors deferred due to low Hb. Based on our results, the determination of the algorithm in the iron deficiency detection is necessary, while the capillary method (HemoCue) represents a more convenient method for Hb testing prior to blood donation.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Ispitivanje statusa gvožđa kod davalaca krvi vraćenih zbog niskog nivoa hemoglobina
T1  - Iron status among blood donors deferred due to low haemoglobin level
EP  - 206
IS  - 2
SP  - 202
VL  - 78
DO  - 10.2298/VSP190327063K
ER  - 

@article{
author = "Kovač, Mirjana and Erić, Bojana and Stojneva-Istatkov, Jelena and Lukić, Vojislav and Milić, Ana and Vukičević, Dragana and Orlić, Dušan and Tomić, Branko",
year = "2021",
abstract = "Uvod/Cilj. Određivanje nivoa hemoglobin (Hb) je rutinski deo selekcije dobrovoljnih davalaca krvi. Prethodno publikovane studije pokazale su da se nedostatak gvožđa javlja kod redovnih davalaca krvi. Cilj ove prospektivne studije je bio da se utvrdi status gvožđa kod davalaca kod kojih je pre davanja utvrđen nizak nivo Hb i da se proceni stepen korelacije kapilarnih metoda, sa referentnom metodom određivanja Hb iz venske krvi, kao i sa i nivoom feritina. Metode. U periodu od februara 2017. do decembra 2018. godine, bilo je uključeno 200 dobrovoljnih davalaca sa niskim nivoima Hb, starosne dobi 19-64 godine (medijana 39 godina). Hb je određivan primenom metode bakar sulfat, kapilarnom metodom "HemoCue", i iz venske krvi u sklopu određivanja kompletne krvne slike (KKS). Nivo feritina određivan je primenom turbodimetrijske metode. Rezultati. Nizak nivo feritina utvrđen je kod 42,7% muškaraca i 57,3% žena (p = 0,008). U odnosu na nivo feritina  lt  12 mg/L, odnosno  gt  50 mg/L, zabeležena je značajna razlika između polova (p = 0,023, odnosno p = 0,022). Poređenje vrednosti Hb dobijenih kapilarnim metodama u odnosu na referentne vrednosti Hb, određene iz KKS, pokazalo je da metoda s bakar sulfatom daje lažno niske vrednosti Hb kod 10,5% slučajeva (p  lt  0,001). Vrednosti Hb dobijene metodom "HemoCue-a" značajno su korelirale sa vrednostima Hb iz KKS, dok korelacija između nivoa feritina i Hb, određenog pomoću obe kapilarne metode, nije uočena. Zaključak. Kod 51,5% naših davalaca krvi koji su vraćeni zbog niskih vrednosti Hb utvrđen je snižen nivo feritina. Na osnovu ovih rezultata neophodno je odrediti algoritam za detekciju nedostatka gvožđa, dok je kapilarna metoda (HemoCue) pogodnija metoda za testiranje Hb pre davanja krvi., Background/Aim. Haemoglobin (Hb) determination is a routine part of the blood donor selection process. Previously reported studies have revealed that iron deficiency is common in frequent donors. This prospective investigation was aimed at examining iron status among blood donors with low circulating Hb and evaluating correlation between Hb values determined by capillary methods and those obtained by reference method from venous blood count (BC), as well as ferritin level. Methods. Between February 2017 and December 2018, 200 consecutively recruited regular blood donors with low Hb, aged 19 to 64 years (median 39), were included. Hb level was determined using the copper sulphate method, the HemoCue capillary method, and also from venous blood within the complete blood count (CBC) test. Plasma ferritin was determined turbidimetrically. Results. In 42.7% of men and 57.3% of women, ferritin concentration was low (p = 0.008). The relative numbers of males and females, with levels  lt  12 mg/L (p = 0.023) or  gt  50 mg/L (p = 0.022), differed. Comparison of the values obtained with the capillary methods with reference Hb levels obtained from the CBC test showed that the copper sulphate procedure gave false fails in 10.5% of cases (p  lt  0.001). Hb values from HemoCue were significantly correlated with Hb values from the CBC test, but no correlation was observed between ferritin levels and Hb levels determined by both capillary method. Conclusion. Low ferritin was observed in 51.5% of Serbian blood donors deferred due to low Hb. Based on our results, the determination of the algorithm in the iron deficiency detection is necessary, while the capillary method (HemoCue) represents a more convenient method for Hb testing prior to blood donation.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Ispitivanje statusa gvožđa kod davalaca krvi vraćenih zbog niskog nivoa hemoglobina, Iron status among blood donors deferred due to low haemoglobin level",
pages = "206-202",
number = "2",
volume = "78",
doi = "10.2298/VSP190327063K"
}

Kovač, M., Erić, B., Stojneva-Istatkov, J., Lukić, V., Milić, A., Vukičević, D., Orlić, D.,& Tomić, B.. (2021). Ispitivanje statusa gvožđa kod davalaca krvi vraćenih zbog niskog nivoa hemoglobina. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 78(2), 202-206.
https://doi.org/10.2298/VSP190327063K

Kovač M, Erić B, Stojneva-Istatkov J, Lukić V, Milić A, Vukičević D, Orlić D, Tomić B. Ispitivanje statusa gvožđa kod davalaca krvi vraćenih zbog niskog nivoa hemoglobina. in Vojnosanitetski pregled. 2021;78(2):202-206.
doi:10.2298/VSP190327063K .

Kovač, Mirjana, Erić, Bojana, Stojneva-Istatkov, Jelena, Lukić, Vojislav, Milić, Ana, Vukičević, Dragana, Orlić, Dušan, Tomić, Branko, "Ispitivanje statusa gvožđa kod davalaca krvi vraćenih zbog niskog nivoa hemoglobina" in Vojnosanitetski pregled, 78, no. 2 (2021):202-206,
https://doi.org/10.2298/VSP190327063K . .

TY  - JOUR
AU  - Piazzi, Manuela
AU  - Kojić, Snežana
AU  - Capanni, Cristina
AU  - Stamenković, Nemanja
AU  - Bavelloni, Alberto
AU  - Marin, Oriano
AU  - Lattanzi, Giovanna
AU  - Blalock, William
AU  - Cenni, Vittoria
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1486
AB  - Simple Summary Osteosarcoma is a rare malignancy of bone, primarily affecting children and young adults. The main objective of this study was to identify novel therapeutic targets to fight the progression of this insidious disease. To this aim, the role of Ankrd2, a stress- and mechano- sensor protein known for being mostly expressed in muscle fibers, was analyzed in the modulation of osteosarcoma progression. By subjecting human osteosarcoma cell lines expressing or silencing Ankrd2 to several functional assays, our results demonstrated that Ankrd2 is involved in the pathogenesis of this cancer. Nonetheless, due to observations obtained by other studies in other model systems, our findings also suggest that Ankrd2 might behave as a "double-faced" cancer driver gene. Ankrd2 is a protein known for being mainly expressed in muscle fibers, where it participates in the mechanical stress response. Since both myocytes and osteoblasts are mesenchymal-derived cells, we were interested in examining the role of Ankrd2 in the progression of osteosarcoma which features a mechano-stress component. Although having been identified in many tumor-derived cell lines and -tissues, no study has yet described nor hypothesized any involvement for this protein in osteosarcoma tumorigenesis. In this paper, we report that Ankrd2 is expressed in cell lines obtained from human osteosarcoma and demonstrate a contribution by this protein in the pathogenesis of this insidious disease. Ankrd2 involvement in osteosarcoma development was evaluated in clones of Saos2, U2OS, HOS and MG63 cells stably expressing Ankrd2, through the investigation of hallmark processes of cancer cells. Interestingly, we found that exogenous expression of Ankrd2 influenced cellular growth, migration and clonogenicity in a cell line-dependent manner, whereas it was able to improve the formation of 3D spheroids in three out of four cellular models and enhanced matrix metalloproteinase (MMP) activity in all tested cell lines. Conversely, downregulation of Ankrd2 expression remarkably reduced proliferation and clonogenic potential of parental cells. As a whole, our data present Ankrd2 as a novel player in osteosarcoma development, opening up new therapeutic perspectives.
PB  - MDPI, Basel
T2  - Cancers
T1  - Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells
IS  - 2
VL  - 13
DO  - 10.3390/cancers13020174
ER  - 

@article{
author = "Piazzi, Manuela and Kojić, Snežana and Capanni, Cristina and Stamenković, Nemanja and Bavelloni, Alberto and Marin, Oriano and Lattanzi, Giovanna and Blalock, William and Cenni, Vittoria",
year = "2021",
abstract = "Simple Summary Osteosarcoma is a rare malignancy of bone, primarily affecting children and young adults. The main objective of this study was to identify novel therapeutic targets to fight the progression of this insidious disease. To this aim, the role of Ankrd2, a stress- and mechano- sensor protein known for being mostly expressed in muscle fibers, was analyzed in the modulation of osteosarcoma progression. By subjecting human osteosarcoma cell lines expressing or silencing Ankrd2 to several functional assays, our results demonstrated that Ankrd2 is involved in the pathogenesis of this cancer. Nonetheless, due to observations obtained by other studies in other model systems, our findings also suggest that Ankrd2 might behave as a "double-faced" cancer driver gene. Ankrd2 is a protein known for being mainly expressed in muscle fibers, where it participates in the mechanical stress response. Since both myocytes and osteoblasts are mesenchymal-derived cells, we were interested in examining the role of Ankrd2 in the progression of osteosarcoma which features a mechano-stress component. Although having been identified in many tumor-derived cell lines and -tissues, no study has yet described nor hypothesized any involvement for this protein in osteosarcoma tumorigenesis. In this paper, we report that Ankrd2 is expressed in cell lines obtained from human osteosarcoma and demonstrate a contribution by this protein in the pathogenesis of this insidious disease. Ankrd2 involvement in osteosarcoma development was evaluated in clones of Saos2, U2OS, HOS and MG63 cells stably expressing Ankrd2, through the investigation of hallmark processes of cancer cells. Interestingly, we found that exogenous expression of Ankrd2 influenced cellular growth, migration and clonogenicity in a cell line-dependent manner, whereas it was able to improve the formation of 3D spheroids in three out of four cellular models and enhanced matrix metalloproteinase (MMP) activity in all tested cell lines. Conversely, downregulation of Ankrd2 expression remarkably reduced proliferation and clonogenic potential of parental cells. As a whole, our data present Ankrd2 as a novel player in osteosarcoma development, opening up new therapeutic perspectives.",
publisher = "MDPI, Basel",
journal = "Cancers",
title = "Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells",
number = "2",
volume = "13",
doi = "10.3390/cancers13020174"
}

Piazzi, M., Kojić, S., Capanni, C., Stamenković, N., Bavelloni, A., Marin, O., Lattanzi, G., Blalock, W.,& Cenni, V.. (2021). Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells. in Cancers
MDPI, Basel., 13(2).
https://doi.org/10.3390/cancers13020174

Piazzi M, Kojić S, Capanni C, Stamenković N, Bavelloni A, Marin O, Lattanzi G, Blalock W, Cenni V. Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells. in Cancers. 2021;13(2).
doi:10.3390/cancers13020174 .

Piazzi, Manuela, Kojić, Snežana, Capanni, Cristina, Stamenković, Nemanja, Bavelloni, Alberto, Marin, Oriano, Lattanzi, Giovanna, Blalock, William, Cenni, Vittoria, "Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells" in Cancers, 13, no. 2 (2021),
https://doi.org/10.3390/cancers13020174 . .

TY  - JOUR
AU  - Despotović, Jovana
AU  - Dragičević, Sandra
AU  - Nikolić, Aleksandra
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1421
AB  - Metastatic colorectal cancer (mCRC) patients are treated with standard chemotherapeutic drugs in the form of FOLFOX and FOLFIRI regimens. There are no reliable markers that could predict response to chemotherapy for mCRC. TGF-beta signaling which interacts with microRNA (miRNA) network has important roles in tumor progression and chemotherapy resistance, thus the interplay between TGF-beta signaling and miRNAs could be crucial for treatment response. The aim of this study was to analyze the effect of chemotherapy for mCRC on TGF-beta signaling and related miRNAs. Hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p were selected out of 316 miRNAs with multiple targets within the TGF-beta signaling by in silico analysis. SW620 cells were treated with chemotherapeutic drugs for mCRC for 1, 3 and 6 days and expression of selected miRNAs, PAI-1, CDH1 and VIM was measured. Expression of TGF-beta signaling-related hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p was time-dependently altered in SW620 cells treated with chemotherapeutics for mCRC. The expression of hsa-miR-93-5p remained downregulated after 6 days under combined treatments FOX and FIRI as well as the hsa-miR-17-5p expression under FIRI. Chemotherapy regimens for mCRC increased expression of a major TGF-beta signaling target gene PAI-1, independently of the selected miRNAs expression. These treatments also increased the expression of epithelial-mesenchymal transition (EMT) markers CDH1 and VIM on day 3 resulting in decrease of mesenchymal-like characteristics. However, their expression returned close to basal level on day 6. In conclusion, after initial response to chemotherapeutic drugs SW620 cells start to return close to the basal mesenchymal state while the long-term downregulated expression pattern of hsa-miR-93-5p and hsa-miR-17-5p makes them candidates worth testing as biomarkers for monitoring combined chemotherapeutic treatments therapy response in mCRC patients.
PB  - Humana Press Inc, Totowa
T2  - Cell Biochemistry and Biophysics
T1  - Effects of Chemotherapy for Metastatic Colorectal Cancer on the TGF-beta Signaling and Related miRNAs hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p
EP  - 767
IS  - 4
SP  - 757
VL  - 79
DO  - 10.1007/s12013-021-00980-3
ER  - 

@article{
author = "Despotović, Jovana and Dragičević, Sandra and Nikolić, Aleksandra",
year = "2021",
abstract = "Metastatic colorectal cancer (mCRC) patients are treated with standard chemotherapeutic drugs in the form of FOLFOX and FOLFIRI regimens. There are no reliable markers that could predict response to chemotherapy for mCRC. TGF-beta signaling which interacts with microRNA (miRNA) network has important roles in tumor progression and chemotherapy resistance, thus the interplay between TGF-beta signaling and miRNAs could be crucial for treatment response. The aim of this study was to analyze the effect of chemotherapy for mCRC on TGF-beta signaling and related miRNAs. Hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p were selected out of 316 miRNAs with multiple targets within the TGF-beta signaling by in silico analysis. SW620 cells were treated with chemotherapeutic drugs for mCRC for 1, 3 and 6 days and expression of selected miRNAs, PAI-1, CDH1 and VIM was measured. Expression of TGF-beta signaling-related hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p was time-dependently altered in SW620 cells treated with chemotherapeutics for mCRC. The expression of hsa-miR-93-5p remained downregulated after 6 days under combined treatments FOX and FIRI as well as the hsa-miR-17-5p expression under FIRI. Chemotherapy regimens for mCRC increased expression of a major TGF-beta signaling target gene PAI-1, independently of the selected miRNAs expression. These treatments also increased the expression of epithelial-mesenchymal transition (EMT) markers CDH1 and VIM on day 3 resulting in decrease of mesenchymal-like characteristics. However, their expression returned close to basal level on day 6. In conclusion, after initial response to chemotherapeutic drugs SW620 cells start to return close to the basal mesenchymal state while the long-term downregulated expression pattern of hsa-miR-93-5p and hsa-miR-17-5p makes them candidates worth testing as biomarkers for monitoring combined chemotherapeutic treatments therapy response in mCRC patients.",
publisher = "Humana Press Inc, Totowa",
journal = "Cell Biochemistry and Biophysics",
title = "Effects of Chemotherapy for Metastatic Colorectal Cancer on the TGF-beta Signaling and Related miRNAs hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p",
pages = "767-757",
number = "4",
volume = "79",
doi = "10.1007/s12013-021-00980-3"
}

Despotović, J., Dragičević, S.,& Nikolić, A.. (2021). Effects of Chemotherapy for Metastatic Colorectal Cancer on the TGF-beta Signaling and Related miRNAs hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p. in Cell Biochemistry and Biophysics
Humana Press Inc, Totowa., 79(4), 757-767.
https://doi.org/10.1007/s12013-021-00980-3

Despotović J, Dragičević S, Nikolić A. Effects of Chemotherapy for Metastatic Colorectal Cancer on the TGF-beta Signaling and Related miRNAs hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p. in Cell Biochemistry and Biophysics. 2021;79(4):757-767.
doi:10.1007/s12013-021-00980-3 .

Despotović, Jovana, Dragičević, Sandra, Nikolić, Aleksandra, "Effects of Chemotherapy for Metastatic Colorectal Cancer on the TGF-beta Signaling and Related miRNAs hsa-miR-17-5p, hsa-miR-21-5p and hsa-miR-93-5p" in Cell Biochemistry and Biophysics, 79, no. 4 (2021):757-767,
https://doi.org/10.1007/s12013-021-00980-3 . .

TY  - JOUR
AU  - Bošković, Srđan
AU  - Juez, Ruben Marin
AU  - Stamenković, Nemanja
AU  - Radojković, Dragica
AU  - Stainier, Didier Y. R.
AU  - Kojić, Snežana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1425
AB  - Ankyrin repeat domain 1 (ANKRD1) is a functionally pleiotropic protein found in the nuclei and sarcomeres of cardiac and skeletal muscles, with a proposed role in linking myofibrilar stress and transcriptional regulation. Rapid upregulation of its expression in response to both physiological and pathological stress supports the involvement of ANKRD1 in muscle tissue adaptation and remodeling. However, the exact role of ANKRD1 remains poorly understood. To begin to investigate its function at higher resolution, we have generated and characterized a TgBAC(ankrd1a:EGFP) zebrafish line. This reporter line displays transgene expression in slow skeletal muscle fibers during development and exercise responsiveness in adult cardiac muscle. To better understand the role of Ankrd1a in pathological conditions in adult zebrafish, we assessed ankrd1a expression after cardiac ventricle cryoinjury and observed localized upregulation in cardiomyocytes in the border zone. We show that this expression in injured hearts is recapitulated by the TgBAC(ankrd1a:EGFP) reporter. Our results identify novel expression domains of ankrd1a and suggest an important role for Ankrd1a in the early stress response and regeneration of cardiac tissue. This new reporter line will help decipher the role of Ankrd1a in striated muscle stress response, including after cardiac injury.
PB  - Elsevier, Amsterdam
T2  - Gene
T1  - The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish
VL  - 792
DO  - 10.1016/j.gene.2021.145725
ER  - 

@article{
author = "Bošković, Srđan and Juez, Ruben Marin and Stamenković, Nemanja and Radojković, Dragica and Stainier, Didier Y. R. and Kojić, Snežana",
year = "2021",
abstract = "Ankyrin repeat domain 1 (ANKRD1) is a functionally pleiotropic protein found in the nuclei and sarcomeres of cardiac and skeletal muscles, with a proposed role in linking myofibrilar stress and transcriptional regulation. Rapid upregulation of its expression in response to both physiological and pathological stress supports the involvement of ANKRD1 in muscle tissue adaptation and remodeling. However, the exact role of ANKRD1 remains poorly understood. To begin to investigate its function at higher resolution, we have generated and characterized a TgBAC(ankrd1a:EGFP) zebrafish line. This reporter line displays transgene expression in slow skeletal muscle fibers during development and exercise responsiveness in adult cardiac muscle. To better understand the role of Ankrd1a in pathological conditions in adult zebrafish, we assessed ankrd1a expression after cardiac ventricle cryoinjury and observed localized upregulation in cardiomyocytes in the border zone. We show that this expression in injured hearts is recapitulated by the TgBAC(ankrd1a:EGFP) reporter. Our results identify novel expression domains of ankrd1a and suggest an important role for Ankrd1a in the early stress response and regeneration of cardiac tissue. This new reporter line will help decipher the role of Ankrd1a in striated muscle stress response, including after cardiac injury.",
publisher = "Elsevier, Amsterdam",
journal = "Gene",
title = "The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish",
volume = "792",
doi = "10.1016/j.gene.2021.145725"
}

Bošković, S., Juez, R. M., Stamenković, N., Radojković, D., Stainier, D. Y. R.,& Kojić, S.. (2021). The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish. in Gene
Elsevier, Amsterdam., 792.
https://doi.org/10.1016/j.gene.2021.145725

Bošković S, Juez RM, Stamenković N, Radojković D, Stainier DYR, Kojić S. The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish. in Gene. 2021;792.
doi:10.1016/j.gene.2021.145725 .

Bošković, Srđan, Juez, Ruben Marin, Stamenković, Nemanja, Radojković, Dragica, Stainier, Didier Y. R., Kojić, Snežana, "The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish" in Gene, 792 (2021),
https://doi.org/10.1016/j.gene.2021.145725 . .

TY  - JOUR
AU  - Nesović, Marija
AU  - Divac Rankov, Aleksandra
AU  - Podolski-Renić, Ana
AU  - Nikolić, Igor
AU  - Tasić, Goran
AU  - Mancini, Arianna
AU  - Schenone, Silvia
AU  - Pesić, Milica
AU  - Dinić, Jelena
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1315
AB  - Glioblastoma (GBM), as the most aggressive brain tumor, displays a high expression of Src tyrosine kinase, which is involved in the survival, migration, and invasiveness of tumor cells. Thus, Src emerged as a potential target for GBM therapy. The effects of Src inhibitors pyrazolo[3,4-d]pyrimidines, Si306 and its prodrug pro-Si306 were investigated in human GBM cell lines (U87 and U87-TxR) and three primary GBM cell cultures. Primary GBM cells were more resistant to Si306 and pro-Si306 according to the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. However, the ability of all GBM cells to degrade the extracellular matrix was considerably compromised after Si306 and pro-Si306 applications. Besides reducing the phosphorylation of Src and its downstream signaling pathway components, both compounds decreased the phosphorylated form of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) expression, showing the potential to suppress the aggressiveness of GBM. In vivo, Si306 and pro-Si306 displayed an anti-invasive effect against U87 xenografts in the zebrafish embryo model. Considering that Si306 and pro-Si306 are able to cross the blood-brain barrier and suppress the spread of GBM cells, we anticipate their clinical testing in the near future. Moreover, the prodrug showed similar efficacy to the drug, implying the rationality of its use in clinical settings.
PB  - MDPI, Basel
T2  - Cancers
T1  - Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo
IS  - 6
VL  - 12
DO  - 10.3390/cancers12061570
ER  - 

@article{
author = "Nesović, Marija and Divac Rankov, Aleksandra and Podolski-Renić, Ana and Nikolić, Igor and Tasić, Goran and Mancini, Arianna and Schenone, Silvia and Pesić, Milica and Dinić, Jelena",
year = "2020",
abstract = "Glioblastoma (GBM), as the most aggressive brain tumor, displays a high expression of Src tyrosine kinase, which is involved in the survival, migration, and invasiveness of tumor cells. Thus, Src emerged as a potential target for GBM therapy. The effects of Src inhibitors pyrazolo[3,4-d]pyrimidines, Si306 and its prodrug pro-Si306 were investigated in human GBM cell lines (U87 and U87-TxR) and three primary GBM cell cultures. Primary GBM cells were more resistant to Si306 and pro-Si306 according to the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. However, the ability of all GBM cells to degrade the extracellular matrix was considerably compromised after Si306 and pro-Si306 applications. Besides reducing the phosphorylation of Src and its downstream signaling pathway components, both compounds decreased the phosphorylated form of focal adhesion kinase (FAK) and epidermal growth factor receptor (EGFR) expression, showing the potential to suppress the aggressiveness of GBM. In vivo, Si306 and pro-Si306 displayed an anti-invasive effect against U87 xenografts in the zebrafish embryo model. Considering that Si306 and pro-Si306 are able to cross the blood-brain barrier and suppress the spread of GBM cells, we anticipate their clinical testing in the near future. Moreover, the prodrug showed similar efficacy to the drug, implying the rationality of its use in clinical settings.",
publisher = "MDPI, Basel",
journal = "Cancers",
title = "Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo",
number = "6",
volume = "12",
doi = "10.3390/cancers12061570"
}

Nesović, M., Divac Rankov, A., Podolski-Renić, A., Nikolić, I., Tasić, G., Mancini, A., Schenone, S., Pesić, M.,& Dinić, J.. (2020). Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo. in Cancers
MDPI, Basel., 12(6).
https://doi.org/10.3390/cancers12061570

Nesović M, Divac Rankov A, Podolski-Renić A, Nikolić I, Tasić G, Mancini A, Schenone S, Pesić M, Dinić J. Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo. in Cancers. 2020;12(6).
doi:10.3390/cancers12061570 .

Nesović, Marija, Divac Rankov, Aleksandra, Podolski-Renić, Ana, Nikolić, Igor, Tasić, Goran, Mancini, Arianna, Schenone, Silvia, Pesić, Milica, Dinić, Jelena, "Src Inhibitors Pyrazolo[3,4-d]pyrimidines, Si306 and Pro-Si306, Inhibit Focal Adhesion Kinase and Suppress Human Glioblastoma Invasion In Vitro and In Vivo" in Cancers, 12, no. 6 (2020),
https://doi.org/10.3390/cancers12061570 . .

TY  - JOUR
AU  - Stamenković, Nemanja
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Bošković, Srđan
AU  - Kojić, Ana
AU  - Popić, Kristina
AU  - Stanković, Marija
AU  - Wang, Yajun
AU  - Milenković, Sanja
AU  - Radojković, Dragica
AU  - Ma, Guada
AU  - Kojić, Snežana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1307
AB  - Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chickenANKRD2. The chickenANKRD2coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative-glycolytic, type IIA myofibers. Association of chickenANKRD2with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests thatANKRD2is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus
EP  - 396
IS  - 4
SP  - 383
VL  - 154
DO  - 10.1007/s00418-020-01899-1
ER  - 

@article{
author = "Stamenković, Nemanja and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Bošković, Srđan and Kojić, Ana and Popić, Kristina and Stanković, Marija and Wang, Yajun and Milenković, Sanja and Radojković, Dragica and Ma, Guada and Kojić, Snežana",
year = "2020",
abstract = "Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chickenANKRD2. The chickenANKRD2coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative-glycolytic, type IIA myofibers. Association of chickenANKRD2with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests thatANKRD2is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus",
pages = "396-383",
number = "4",
volume = "154",
doi = "10.1007/s00418-020-01899-1"
}

Stamenković, N., Jasnić, J., Novković, M., Milošević, E., Bošković, S., Kojić, A., Popić, K., Stanković, M., Wang, Y., Milenković, S., Radojković, D., Ma, G.,& Kojić, S.. (2020). Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus. in Histochemistry and Cell Biology
Springer, New York., 154(4), 383-396.
https://doi.org/10.1007/s00418-020-01899-1

Stamenković N, Jasnić J, Novković M, Milošević E, Bošković S, Kojić A, Popić K, Stanković M, Wang Y, Milenković S, Radojković D, Ma G, Kojić S. Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus. in Histochemistry and Cell Biology. 2020;154(4):383-396.
doi:10.1007/s00418-020-01899-1 .

Stamenković, Nemanja, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Bošković, Srđan, Kojić, Ana, Popić, Kristina, Stanković, Marija, Wang, Yajun, Milenković, Sanja, Radojković, Dragica, Ma, Guada, Kojić, Snežana, "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus" in Histochemistry and Cell Biology, 154, no. 4 (2020):383-396,
https://doi.org/10.1007/s00418-020-01899-1 . .

TY  - JOUR
AU  - Pruner, Iva
AU  - Farm, Maria
AU  - Tomić, Branko
AU  - Gvozdenov, Maja
AU  - Kovač, Mirjana
AU  - Miljić, Predrag
AU  - Soutari, Nida Mahmoud Hourani
AU  - Antović, Aleksandra
AU  - Radojković, Dragica
AU  - Antović, Jovan P.
AU  - Đorđević, Valentina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1375
AB  - BACKGROUND: Thrombosis is a major global disease burden with almost 60% of cases related to underlying heredity and most cases still idiopathic. Synonymous single nucleotide polymorphisms (sSNPs) are considered silent and phenotypically neutral. Our previous study revealed a novel synonymous FII c.1824C gt  T variant as a potential risk factor for pregnancy loss, but it has not yet been associated with thrombotic diseases. METHODS: To determine the frequency of the FII c.1824C gt  T variant we have sequenced patients' DNA. Prothrombin RNA expression was measured by quantitative PCR. Functional analyses included routine hemostasis tests, western blotting and ELISA to determine prothrombin levels in plasma, and global hemostasis assays for thrombin and fibrin generation in carriers of the FII c.1824C gt  T variant. Scanning electron mi- croscopy was used to examine the structure of fibrin clots. RESULTS: Frequency of the FII c.1824C gt  T variant was significantly increased in patients with venous thromboembolism and cerebrovascular insult. Examination in vitro demonstrated increased expression of prothrombin mRNA in FII c.1824C gt  T transfected cells. Our ex vivo study of FII c.1824C gt  T carriers showed that the presence of this variant was associated with hyperprothrom-binemia, hypofibrinolysis, and formation of densely packed fibrin clots resistant to fibrinolysis. CONCLUSION: Our data indicate that FII c.1824C gt  T, although a synonymous variant, leads to the development of a prothrombotic phenotype and could represent a new prothrombotic risk factor. As a silent variant, FII c.1824C gt  T would probably be overlooked during genetic screening, and our results show that it could not be detected in routine laboratory tests.
PB  - Oxford Univ Press Inc, Cary
T2  - Clinical Chemistry
T1  - The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor
EP  - 389
IS  - 2
SP  - 379
VL  - 66
DO  - 10.1093/clinchem/hvz015
ER  - 

@article{
author = "Pruner, Iva and Farm, Maria and Tomić, Branko and Gvozdenov, Maja and Kovač, Mirjana and Miljić, Predrag and Soutari, Nida Mahmoud Hourani and Antović, Aleksandra and Radojković, Dragica and Antović, Jovan P. and Đorđević, Valentina",
year = "2020",
abstract = "BACKGROUND: Thrombosis is a major global disease burden with almost 60% of cases related to underlying heredity and most cases still idiopathic. Synonymous single nucleotide polymorphisms (sSNPs) are considered silent and phenotypically neutral. Our previous study revealed a novel synonymous FII c.1824C gt  T variant as a potential risk factor for pregnancy loss, but it has not yet been associated with thrombotic diseases. METHODS: To determine the frequency of the FII c.1824C gt  T variant we have sequenced patients' DNA. Prothrombin RNA expression was measured by quantitative PCR. Functional analyses included routine hemostasis tests, western blotting and ELISA to determine prothrombin levels in plasma, and global hemostasis assays for thrombin and fibrin generation in carriers of the FII c.1824C gt  T variant. Scanning electron mi- croscopy was used to examine the structure of fibrin clots. RESULTS: Frequency of the FII c.1824C gt  T variant was significantly increased in patients with venous thromboembolism and cerebrovascular insult. Examination in vitro demonstrated increased expression of prothrombin mRNA in FII c.1824C gt  T transfected cells. Our ex vivo study of FII c.1824C gt  T carriers showed that the presence of this variant was associated with hyperprothrom-binemia, hypofibrinolysis, and formation of densely packed fibrin clots resistant to fibrinolysis. CONCLUSION: Our data indicate that FII c.1824C gt  T, although a synonymous variant, leads to the development of a prothrombotic phenotype and could represent a new prothrombotic risk factor. As a silent variant, FII c.1824C gt  T would probably be overlooked during genetic screening, and our results show that it could not be detected in routine laboratory tests.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Clinical Chemistry",
title = "The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor",
pages = "389-379",
number = "2",
volume = "66",
doi = "10.1093/clinchem/hvz015"
}

Pruner, I., Farm, M., Tomić, B., Gvozdenov, M., Kovač, M., Miljić, P., Soutari, N. M. H., Antović, A., Radojković, D., Antović, J. P.,& Đorđević, V.. (2020). The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor. in Clinical Chemistry
Oxford Univ Press Inc, Cary., 66(2), 379-389.
https://doi.org/10.1093/clinchem/hvz015

Pruner I, Farm M, Tomić B, Gvozdenov M, Kovač M, Miljić P, Soutari NMH, Antović A, Radojković D, Antović JP, Đorđević V. The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor. in Clinical Chemistry. 2020;66(2):379-389.
doi:10.1093/clinchem/hvz015 .

Pruner, Iva, Farm, Maria, Tomić, Branko, Gvozdenov, Maja, Kovač, Mirjana, Miljić, Predrag, Soutari, Nida Mahmoud Hourani, Antović, Aleksandra, Radojković, Dragica, Antović, Jovan P., Đorđević, Valentina, "The Silence Speaks, but We Do Not Listen: Synonymous c.1824C  gt  T Gene Variant in the Last Exon of the Prothrombin Gene as a New Prothrombotic Risk Factor" in Clinical Chemistry, 66, no. 2 (2020):379-389,
https://doi.org/10.1093/clinchem/hvz015 . .

TY  - JOUR
AU  - Dragičević, Sandra
AU  - Kovacević, Draginja
AU  - Divac Rankov, Aleksandra
AU  - Nikolić, Aleksandra
AU  - Radojković, Dragica
AU  - Radović, Svetlana
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1233
AB  - Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders, whereas the use of V. thapsus needs further investigation.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells
EP  - 416
IS  - 3
SP  - 409
VL  - 71
DO  - 10.2298/ABS181213022D
ER  - 

@article{
author = "Dragičević, Sandra and Kovacević, Draginja and Divac Rankov, Aleksandra and Nikolić, Aleksandra and Radojković, Dragica and Radović, Svetlana",
year = "2019",
abstract = "Tussilago farfara (coltsfoot) and Verbascum thapsus (mullein) have been used as folk remedies for treating respiratory disorders. The aim of this study was to test the toxicity of the water extracts of T. farfara and V. thapsus in vivo in zebrafish and in vitro in BEAS 2B epithelial bronchial cells. To the best of our knowledge, this is the first study to investigate the antioxidative properties of T. farfara and V. thapsus extracts in cell culture. Our results show that the T. farfara leaf extract does not produce toxic effects on zebrafish embryos or BEAS 2B cells, and that it has a protective effect in BEAS 2B after induction of oxidative stress. The water extract from V. thapsus displayed pronounced toxic effects on zebrafish embryos and BEAS 2B cells and did not exhibit a significant antioxidative effect on BEAS 2B cells exposed to oxidative stress. Our results suggest that the use of T. farfara water leaf extract is potentially safe and effective in treating respiratory disorders, whereas the use of V. thapsus needs further investigation.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells",
pages = "416-409",
number = "3",
volume = "71",
doi = "10.2298/ABS181213022D"
}

Dragičević, S., Kovacević, D., Divac Rankov, A., Nikolić, A., Radojković, D.,& Radović, S.. (2019). Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(3), 409-416.
https://doi.org/10.2298/ABS181213022D

Dragičević S, Kovacević D, Divac Rankov A, Nikolić A, Radojković D, Radović S. Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells. in Archives of Biological Sciences. 2019;71(3):409-416.
doi:10.2298/ABS181213022D .

Dragičević, Sandra, Kovacević, Draginja, Divac Rankov, Aleksandra, Nikolić, Aleksandra, Radojković, Dragica, Radović, Svetlana, "Evaluation of toxicity and antioxidative effects of Tussilago farfara and Verbascum thapsus water extracts in zebrafish and in bronchial epithelial cells" in Archives of Biological Sciences, 71, no. 3 (2019):409-416,
https://doi.org/10.2298/ABS181213022D . .

TY  - JOUR
AU  - Rakićević, Ljiljana
AU  - Nestorović, Aleksandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1277
AB  - Genetics has given a real boost to personalized and precision medicine, providing data used either for precise diagnostics, prediction of the course of illness, or for selecting therapy and tailoring it. Pharmacogenetics, as a discipline researching connection between genetic background of an individual and the effect of a certain drug, has created new possibilities in medicine. One of the most researched drugs in pharmacogenetics is certainly clopidogrel.
PB  - Wiley, Hoboken
T2  - Clinical Pharmacology & Therapeutics
T1  - Pharmacogenetics of Clopidogrel Therapy and Neurointerventional Procedures: We Need Precision Data for Precision Medicine
EP  - 549
IS  - 3
SP  - 547
VL  - 105
DO  - 10.1002/cpt.1105
ER  - 

@article{
author = "Rakićević, Ljiljana and Nestorović, Aleksandra",
year = "2019",
abstract = "Genetics has given a real boost to personalized and precision medicine, providing data used either for precise diagnostics, prediction of the course of illness, or for selecting therapy and tailoring it. Pharmacogenetics, as a discipline researching connection between genetic background of an individual and the effect of a certain drug, has created new possibilities in medicine. One of the most researched drugs in pharmacogenetics is certainly clopidogrel.",
publisher = "Wiley, Hoboken",
journal = "Clinical Pharmacology & Therapeutics",
title = "Pharmacogenetics of Clopidogrel Therapy and Neurointerventional Procedures: We Need Precision Data for Precision Medicine",
pages = "549-547",
number = "3",
volume = "105",
doi = "10.1002/cpt.1105"
}

Rakićević, L.,& Nestorović, A.. (2019). Pharmacogenetics of Clopidogrel Therapy and Neurointerventional Procedures: We Need Precision Data for Precision Medicine. in Clinical Pharmacology & Therapeutics
Wiley, Hoboken., 105(3), 547-549.
https://doi.org/10.1002/cpt.1105

Rakićević L, Nestorović A. Pharmacogenetics of Clopidogrel Therapy and Neurointerventional Procedures: We Need Precision Data for Precision Medicine. in Clinical Pharmacology & Therapeutics. 2019;105(3):547-549.
doi:10.1002/cpt.1105 .

Rakićević, Ljiljana, Nestorović, Aleksandra, "Pharmacogenetics of Clopidogrel Therapy and Neurointerventional Procedures: We Need Precision Data for Precision Medicine" in Clinical Pharmacology & Therapeutics, 105, no. 3 (2019):547-549,
https://doi.org/10.1002/cpt.1105 . .

TY  - JOUR
AU  - Pruner, Iva
AU  - Tomić, Branko
AU  - Dragojević, Marija
AU  - Gvozdenov, Maja
AU  - Kovač, Mirjana
AU  - Radojković, Dragica
AU  - Đorđević, Valentina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1209
AB  - Breast cancer is the leading cause of cancer-related death among women. An increased burden of thrombotic events among breast cancer patients, leading to higher mortality and morbidity rates, is well established. There are a number of genetic risk factors associated with thrombosis, but their contribution to thrombotic tendencies in patients with cancer is not completely elucidated. We aimed to investigate possible role of FV Leiden, FII G20210A, MTHFR C677T and PAI-1 4G/5G gene variants in etiopathology of breast cancer and accompanying thrombosis in cohort of Serbian patients. Our study included 316 subject divided in three groups: breast cancer patients with (97) or without (99) accompanying thrombosis and healthy control group (120). According to our results, the prevalence for all four prothrombotic gene variants were similar in cancer patients with and without thrombosis and no statistically significant difference was observed between these groups. We detected lower frequency of MTHFR 677TT genotype in breast cancer patients when compared to control group (P=0.014; OR=0.145 (95%CI 0.031-0.679)), indicated that MTHFR C677T homozygosity could play a protective role in breast cancer susceptibility. Our study noted the lack of association between common prothrombotic gene variants and increased prothrombotic risk in Serbian breast cancer patients. Also, our results point out possible role of MTHFR 677TT genotype in etiology of breast cancer, but further studies on larger cohort of patients are needed.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Inherited thrombophilic risk factors in Serbian breast cancer patients
EP  - 472
IS  - 2
SP  - 463
VL  - 51
DO  - 10.2298/GENSR1902463P
ER  - 

@article{
author = "Pruner, Iva and Tomić, Branko and Dragojević, Marija and Gvozdenov, Maja and Kovač, Mirjana and Radojković, Dragica and Đorđević, Valentina",
year = "2019",
abstract = "Breast cancer is the leading cause of cancer-related death among women. An increased burden of thrombotic events among breast cancer patients, leading to higher mortality and morbidity rates, is well established. There are a number of genetic risk factors associated with thrombosis, but their contribution to thrombotic tendencies in patients with cancer is not completely elucidated. We aimed to investigate possible role of FV Leiden, FII G20210A, MTHFR C677T and PAI-1 4G/5G gene variants in etiopathology of breast cancer and accompanying thrombosis in cohort of Serbian patients. Our study included 316 subject divided in three groups: breast cancer patients with (97) or without (99) accompanying thrombosis and healthy control group (120). According to our results, the prevalence for all four prothrombotic gene variants were similar in cancer patients with and without thrombosis and no statistically significant difference was observed between these groups. We detected lower frequency of MTHFR 677TT genotype in breast cancer patients when compared to control group (P=0.014; OR=0.145 (95%CI 0.031-0.679)), indicated that MTHFR C677T homozygosity could play a protective role in breast cancer susceptibility. Our study noted the lack of association between common prothrombotic gene variants and increased prothrombotic risk in Serbian breast cancer patients. Also, our results point out possible role of MTHFR 677TT genotype in etiology of breast cancer, but further studies on larger cohort of patients are needed.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Inherited thrombophilic risk factors in Serbian breast cancer patients",
pages = "472-463",
number = "2",
volume = "51",
doi = "10.2298/GENSR1902463P"
}

Pruner, I., Tomić, B., Dragojević, M., Gvozdenov, M., Kovač, M., Radojković, D.,& Đorđević, V.. (2019). Inherited thrombophilic risk factors in Serbian breast cancer patients. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 51(2), 463-472.
https://doi.org/10.2298/GENSR1902463P

Pruner I, Tomić B, Dragojević M, Gvozdenov M, Kovač M, Radojković D, Đorđević V. Inherited thrombophilic risk factors in Serbian breast cancer patients. in Genetika-Belgrade. 2019;51(2):463-472.
doi:10.2298/GENSR1902463P .

Pruner, Iva, Tomić, Branko, Dragojević, Marija, Gvozdenov, Maja, Kovač, Mirjana, Radojković, Dragica, Đorđević, Valentina, "Inherited thrombophilic risk factors in Serbian breast cancer patients" in Genetika-Belgrade, 51, no. 2 (2019):463-472,
https://doi.org/10.2298/GENSR1902463P . .

TY  - JOUR
AU  - Cumbo, Marija
AU  - Tomić, Branko
AU  - Dunjić Manevski, Sofija
AU  - Jovanović, Tamara
AU  - Gvozdenov, Maja
AU  - Pruner, Iva
AU  - Aralica, Gorana
AU  - Kapitanović, Sanja
AU  - Cacev, Tamara
AU  - Đorđević, Valentina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1219
AB  - Background/ Aim: Thrombin plays significant roles in various types of cancer. However, the expression levels of prothrombin, the thrombin precursor, in cancer remain unclear. Variants of the 3'end of the prothrombin gene lead to increased prothrombin expression. This study aimed to analyze prothrombin 3'end gene variants in colon tumor and adjacent normal tissue samples. Materials and Methods: The study group consisted of 93 patients suffering from colon adenocarcinoma. The 3'end of the prothrombin gene was analyzed by DNA sequencing. Results: Three variants, all previously associated with increased prothrombin expression were detected. Frequency of the FII 19911G allele was 46.77% and 47.85% in tumor and normal tissue, respectively. For the FII 20210A allele, the detected frequencies were 2.15% and 1.61%, respectively. The frequency of the FII c.1824T allele was 0.54% in both tissues. Four patients showed different genotypes in tumor and normal tissue. Conclusion: Prothrombin 3' end gene variants may play a role in colorectal cancer.
PB  - Int Inst Anticancer Research, Athens
T2  - Anticancer Research
T1  - Prothrombin 3'end Gene Variants in Patients With Sporadic Colon Adenocarcinoma
EP  - 6071
IS  - 11
SP  - 6067
VL  - 39
DO  - 10.21873/anticanres.13814
ER  - 

@article{
author = "Cumbo, Marija and Tomić, Branko and Dunjić Manevski, Sofija and Jovanović, Tamara and Gvozdenov, Maja and Pruner, Iva and Aralica, Gorana and Kapitanović, Sanja and Cacev, Tamara and Đorđević, Valentina",
year = "2019",
abstract = "Background/ Aim: Thrombin plays significant roles in various types of cancer. However, the expression levels of prothrombin, the thrombin precursor, in cancer remain unclear. Variants of the 3'end of the prothrombin gene lead to increased prothrombin expression. This study aimed to analyze prothrombin 3'end gene variants in colon tumor and adjacent normal tissue samples. Materials and Methods: The study group consisted of 93 patients suffering from colon adenocarcinoma. The 3'end of the prothrombin gene was analyzed by DNA sequencing. Results: Three variants, all previously associated with increased prothrombin expression were detected. Frequency of the FII 19911G allele was 46.77% and 47.85% in tumor and normal tissue, respectively. For the FII 20210A allele, the detected frequencies were 2.15% and 1.61%, respectively. The frequency of the FII c.1824T allele was 0.54% in both tissues. Four patients showed different genotypes in tumor and normal tissue. Conclusion: Prothrombin 3' end gene variants may play a role in colorectal cancer.",
publisher = "Int Inst Anticancer Research, Athens",
journal = "Anticancer Research",
title = "Prothrombin 3'end Gene Variants in Patients With Sporadic Colon Adenocarcinoma",
pages = "6071-6067",
number = "11",
volume = "39",
doi = "10.21873/anticanres.13814"
}

Cumbo, M., Tomić, B., Dunjić Manevski, S., Jovanović, T., Gvozdenov, M., Pruner, I., Aralica, G., Kapitanović, S., Cacev, T.,& Đorđević, V.. (2019). Prothrombin 3'end Gene Variants in Patients With Sporadic Colon Adenocarcinoma. in Anticancer Research
Int Inst Anticancer Research, Athens., 39(11), 6067-6071.
https://doi.org/10.21873/anticanres.13814

Cumbo M, Tomić B, Dunjić Manevski S, Jovanović T, Gvozdenov M, Pruner I, Aralica G, Kapitanović S, Cacev T, Đorđević V. Prothrombin 3'end Gene Variants in Patients With Sporadic Colon Adenocarcinoma. in Anticancer Research. 2019;39(11):6067-6071.
doi:10.21873/anticanres.13814 .

Cumbo, Marija, Tomić, Branko, Dunjić Manevski, Sofija, Jovanović, Tamara, Gvozdenov, Maja, Pruner, Iva, Aralica, Gorana, Kapitanović, Sanja, Cacev, Tamara, Đorđević, Valentina, "Prothrombin 3'end Gene Variants in Patients With Sporadic Colon Adenocarcinoma" in Anticancer Research, 39, no. 11 (2019):6067-6071,
https://doi.org/10.21873/anticanres.13814 . .

TY  - JOUR
AU  - Kovač, Mirjana
AU  - Kovac, Zeljko
AU  - Tomasević, Zorica
AU  - Tomić, Branko
AU  - Gvozdenov, Maja
AU  - Radojković, Dragica
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1250
PB  - Wiley, Hoboken
T2  - Breast Journal
T1  - Breast cancer and recurrent thrombosis - Results from prospective single center study
EP  - 785
IS  - 4
SP  - 783
VL  - 25
DO  - 10.1111/tbj.13326
ER  - 

@article{
author = "Kovač, Mirjana and Kovac, Zeljko and Tomasević, Zorica and Tomić, Branko and Gvozdenov, Maja and Radojković, Dragica",
year = "2019",
publisher = "Wiley, Hoboken",
journal = "Breast Journal",
title = "Breast cancer and recurrent thrombosis - Results from prospective single center study",
pages = "785-783",
number = "4",
volume = "25",
doi = "10.1111/tbj.13326"
}

Kovač, M., Kovac, Z., Tomasević, Z., Tomić, B., Gvozdenov, M.,& Radojković, D.. (2019). Breast cancer and recurrent thrombosis - Results from prospective single center study. in Breast Journal
Wiley, Hoboken., 25(4), 783-785.
https://doi.org/10.1111/tbj.13326

Kovač M, Kovac Z, Tomasević Z, Tomić B, Gvozdenov M, Radojković D. Breast cancer and recurrent thrombosis - Results from prospective single center study. in Breast Journal. 2019;25(4):783-785.
doi:10.1111/tbj.13326 .

Kovač, Mirjana, Kovac, Zeljko, Tomasević, Zorica, Tomić, Branko, Gvozdenov, Maja, Radojković, Dragica, "Breast cancer and recurrent thrombosis - Results from prospective single center study" in Breast Journal, 25, no. 4 (2019):783-785,
https://doi.org/10.1111/tbj.13326 . .

TY  - JOUR
AU  - Jeremić, Ivica
AU  - Đurić, Olivera
AU  - Nikolić, Milos
AU  - Vlajnić, Marina
AU  - Nikolić, Aleksandra
AU  - Radojković, Dragica
AU  - Bonaci-Nikolić, Branka
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1257
AB  - Neutrophil extracellular traps (NETs) are the main source of autoantigens in systemic lupus erythematosus (SLE). The aim of this study was to evaluate the clinical importance of NETs-associated markers in SLE. We compared NETs-associated markers in SLE patients (n = 111) with healthy controls (n = 50). Moreover, in 35 patients with drug-naive SLE (n = 35), we investigated correlation between NETs-associated markers [DNase I concentration, myeloperoxidase (MPO) activity, anti-MPO antibodies, cell-free DNA (cfDNA), NETolytic activity] with serological parameters [anti-dsDNA antibodies, C3, C4 and B-cell activating factor (BAFF) levels] and disease activity measured by modified SLE Disease Activity Index (M-SLEDAI-2K). In comparison with healthy controls, SLE patients had higher cfDNA, MPO activity, anti-MPO antibodies (p  lt  0.001), BAFF and DNase I concentration (p  lt  0.01). Contrary, NETolytic activity was lower in SLE patients (p  lt  0.05), despite higher concentration of DNase I. MPO activity and cfDNA levels showed correlation with DNase I concentration (p  lt  0.001, p  lt  0.01, respectively). BAFF levels correlated with cfDNA, DNase I concentration and MPO activity (p  lt  0.05). Anti-dsDNA antibodies showed correlation with MPO activity (p  lt  0.01), cfDNA and BAFF levels (p  lt  0.001). Anti-dsDNA and C3 levels were independent predictors of M-SLEDAI-2K in multivariate analysis (p  lt  0.01). We demonstrated that sera of SLE patients have decreased NETolytic activity, leading to increased levels of various NETs-associated markers, which correlate with anti-dsDNA antibodies in drug-naive SLE. We showed that BAFF participates in a complex relationship between NETosis and anti-dsDNA antibodies production. These findings have important implications for a better understanding of SLE pathogenesis and development of therapy that inhibits NETs persistence and disease progression.
PB  - Springer Heidelberg, Heidelberg
T2  - Rheumatology International
T1  - Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus
EP  - 1857
IS  - 11
SP  - 1849
VL  - 39
DO  - 10.1007/s00296-019-04426-1
ER  - 

@article{
author = "Jeremić, Ivica and Đurić, Olivera and Nikolić, Milos and Vlajnić, Marina and Nikolić, Aleksandra and Radojković, Dragica and Bonaci-Nikolić, Branka",
year = "2019",
abstract = "Neutrophil extracellular traps (NETs) are the main source of autoantigens in systemic lupus erythematosus (SLE). The aim of this study was to evaluate the clinical importance of NETs-associated markers in SLE. We compared NETs-associated markers in SLE patients (n = 111) with healthy controls (n = 50). Moreover, in 35 patients with drug-naive SLE (n = 35), we investigated correlation between NETs-associated markers [DNase I concentration, myeloperoxidase (MPO) activity, anti-MPO antibodies, cell-free DNA (cfDNA), NETolytic activity] with serological parameters [anti-dsDNA antibodies, C3, C4 and B-cell activating factor (BAFF) levels] and disease activity measured by modified SLE Disease Activity Index (M-SLEDAI-2K). In comparison with healthy controls, SLE patients had higher cfDNA, MPO activity, anti-MPO antibodies (p  lt  0.001), BAFF and DNase I concentration (p  lt  0.01). Contrary, NETolytic activity was lower in SLE patients (p  lt  0.05), despite higher concentration of DNase I. MPO activity and cfDNA levels showed correlation with DNase I concentration (p  lt  0.001, p  lt  0.01, respectively). BAFF levels correlated with cfDNA, DNase I concentration and MPO activity (p  lt  0.05). Anti-dsDNA antibodies showed correlation with MPO activity (p  lt  0.01), cfDNA and BAFF levels (p  lt  0.001). Anti-dsDNA and C3 levels were independent predictors of M-SLEDAI-2K in multivariate analysis (p  lt  0.01). We demonstrated that sera of SLE patients have decreased NETolytic activity, leading to increased levels of various NETs-associated markers, which correlate with anti-dsDNA antibodies in drug-naive SLE. We showed that BAFF participates in a complex relationship between NETosis and anti-dsDNA antibodies production. These findings have important implications for a better understanding of SLE pathogenesis and development of therapy that inhibits NETs persistence and disease progression.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Rheumatology International",
title = "Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus",
pages = "1857-1849",
number = "11",
volume = "39",
doi = "10.1007/s00296-019-04426-1"
}

Jeremić, I., Đurić, O., Nikolić, M., Vlajnić, M., Nikolić, A., Radojković, D.,& Bonaci-Nikolić, B.. (2019). Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus. in Rheumatology International
Springer Heidelberg, Heidelberg., 39(11), 1849-1857.
https://doi.org/10.1007/s00296-019-04426-1

Jeremić I, Đurić O, Nikolić M, Vlajnić M, Nikolić A, Radojković D, Bonaci-Nikolić B. Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus. in Rheumatology International. 2019;39(11):1849-1857.
doi:10.1007/s00296-019-04426-1 .

Jeremić, Ivica, Đurić, Olivera, Nikolić, Milos, Vlajnić, Marina, Nikolić, Aleksandra, Radojković, Dragica, Bonaci-Nikolić, Branka, "Neutrophil extracellular traps-associated markers are elevated in patients with systemic lupus erythematosus" in Rheumatology International, 39, no. 11 (2019):1849-1857,
https://doi.org/10.1007/s00296-019-04426-1 . .

TY  - JOUR
AU  - Dunjić Manevski, Sofija
AU  - Cumbo, Marija
AU  - Gvozdenov, Maja
AU  - Tomić, Branko
AU  - Pruner, Iva
AU  - Radojković, Dragica
AU  - Đorđević, Valentina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1244
AB  - The link between thrombotic disorders and cancer has been known for over 150 years, although the precise mechanism of this relationship has not yet been resolved. Current data show that thrombin has a significant role in cancer metabolism, invasiveness, adhesion and survival. However, data regarding the expression of the thrombin precursor prothrombin in various cancer cell lines are scarce. Therefore, it was our objective to determine whether common cancer-derived cell lines (Caco-2, MCF-7, SK-BR-3, U-87 and U-251) express prothrombin. The prothrombin RNA expression level was assessed by qPCR, and the presence of prothrombin was analyzed by Western blot analysis. Our results show that Caco-2 cells originating from colorectal adenocarcinoma express prothrombin, whereas other analyzed cell lines do not. Our results provide a background for further research into the role of (pro) thrombin in cancer etiopathology.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Prothrombin expression in cancer-derived cell lines
EP  - 54
IS  - 1
SP  - 49
VL  - 71
DO  - 10.2298/ABS180829046D
ER  - 

@article{
author = "Dunjić Manevski, Sofija and Cumbo, Marija and Gvozdenov, Maja and Tomić, Branko and Pruner, Iva and Radojković, Dragica and Đorđević, Valentina",
year = "2019",
abstract = "The link between thrombotic disorders and cancer has been known for over 150 years, although the precise mechanism of this relationship has not yet been resolved. Current data show that thrombin has a significant role in cancer metabolism, invasiveness, adhesion and survival. However, data regarding the expression of the thrombin precursor prothrombin in various cancer cell lines are scarce. Therefore, it was our objective to determine whether common cancer-derived cell lines (Caco-2, MCF-7, SK-BR-3, U-87 and U-251) express prothrombin. The prothrombin RNA expression level was assessed by qPCR, and the presence of prothrombin was analyzed by Western blot analysis. Our results show that Caco-2 cells originating from colorectal adenocarcinoma express prothrombin, whereas other analyzed cell lines do not. Our results provide a background for further research into the role of (pro) thrombin in cancer etiopathology.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Prothrombin expression in cancer-derived cell lines",
pages = "54-49",
number = "1",
volume = "71",
doi = "10.2298/ABS180829046D"
}

Dunjić Manevski, S., Cumbo, M., Gvozdenov, M., Tomić, B., Pruner, I., Radojković, D.,& Đorđević, V.. (2019). Prothrombin expression in cancer-derived cell lines. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(1), 49-54.
https://doi.org/10.2298/ABS180829046D

Dunjić Manevski S, Cumbo M, Gvozdenov M, Tomić B, Pruner I, Radojković D, Đorđević V. Prothrombin expression in cancer-derived cell lines. in Archives of Biological Sciences. 2019;71(1):49-54.
doi:10.2298/ABS180829046D .

Dunjić Manevski, Sofija, Cumbo, Marija, Gvozdenov, Maja, Tomić, Branko, Pruner, Iva, Radojković, Dragica, Đorđević, Valentina, "Prothrombin expression in cancer-derived cell lines" in Archives of Biological Sciences, 71, no. 1 (2019):49-54,
https://doi.org/10.2298/ABS180829046D . .

TY  - JOUR
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Capanni, Cristina
AU  - Faulkner, Georgine
AU  - Lattanzi, Giovanna
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1282
AB  - Ankrd2 (ankyrin repeats containing domain 2) or Arpp (ankyrin repeat, PEST sequence, and proline-rich region) is a member of the muscle ankyrin repeat protein family. Ankrd2 is mostly expressed in skeletal muscle, where it plays an intriguing role in the transcriptional response to stress induced by mechanical stimulation as well as by cellular reactive oxygen species. Our studies in myoblasts from Emery-Dreifuss muscular dystrophy 2, a LMNA-linked disease affecting skeletal and cardiac muscles, demonstrated that Ankrd2 is a lamin A-binding protein and that mutated lamins found in Emery-Dreifuss muscular dystrophy change the dynamics of Ankrd2 nuclear import, thus affecting oxidative stress response. In this review, besides describing the latest advances related to Ankrd2 studies, including novel discoveries on Ankrd2 isoform-specific functions, we report the main findings on the relationship of Ankrd2 with A-type lamins and discuss known and potential mechanisms involving defective Ankrd2-lamin A interplay in the pathogenesis of muscular laminopathies.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies
VL  - 2019
DO  - 10.1155/2019/7318796
ER  - 

@article{
author = "Cenni, Vittoria and Kojić, Snežana and Capanni, Cristina and Faulkner, Georgine and Lattanzi, Giovanna",
year = "2019",
abstract = "Ankrd2 (ankyrin repeats containing domain 2) or Arpp (ankyrin repeat, PEST sequence, and proline-rich region) is a member of the muscle ankyrin repeat protein family. Ankrd2 is mostly expressed in skeletal muscle, where it plays an intriguing role in the transcriptional response to stress induced by mechanical stimulation as well as by cellular reactive oxygen species. Our studies in myoblasts from Emery-Dreifuss muscular dystrophy 2, a LMNA-linked disease affecting skeletal and cardiac muscles, demonstrated that Ankrd2 is a lamin A-binding protein and that mutated lamins found in Emery-Dreifuss muscular dystrophy change the dynamics of Ankrd2 nuclear import, thus affecting oxidative stress response. In this review, besides describing the latest advances related to Ankrd2 studies, including novel discoveries on Ankrd2 isoform-specific functions, we report the main findings on the relationship of Ankrd2 with A-type lamins and discuss known and potential mechanisms involving defective Ankrd2-lamin A interplay in the pathogenesis of muscular laminopathies.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies",
volume = "2019",
doi = "10.1155/2019/7318796"
}

Cenni, V., Kojić, S., Capanni, C., Faulkner, G.,& Lattanzi, G.. (2019). Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2019.
https://doi.org/10.1155/2019/7318796

Cenni V, Kojić S, Capanni C, Faulkner G, Lattanzi G. Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/7318796 .

Cenni, Vittoria, Kojić, Snežana, Capanni, Cristina, Faulkner, Georgine, Lattanzi, Giovanna, "Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/7318796 . .

TY  - CONF
AU  - Despotović, Jovana
AU  - Nikolić, Aleksandra
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1202
PB  - Nature Publishing Group, London
C3  - European Journal of Human Genetics
T1  - Metastatic colorectal cancer chemotherapeutic drugs alter the expression of TGF beta signaling-related miR-175p, miR-21-5p and miR-93-5p in vitro
EP  - 1582
SP  - 1581
VL  - 27
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1202
ER  - 

@conference{
author = "Despotović, Jovana and Nikolić, Aleksandra",
year = "2019",
publisher = "Nature Publishing Group, London",
journal = "European Journal of Human Genetics",
title = "Metastatic colorectal cancer chemotherapeutic drugs alter the expression of TGF beta signaling-related miR-175p, miR-21-5p and miR-93-5p in vitro",
pages = "1582-1581",
volume = "27",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1202"
}

Despotović, J.,& Nikolić, A.. (2019). Metastatic colorectal cancer chemotherapeutic drugs alter the expression of TGF beta signaling-related miR-175p, miR-21-5p and miR-93-5p in vitro. in European Journal of Human Genetics
Nature Publishing Group, London., 27, 1581-1582.
https://hdl.handle.net/21.15107/rcub_imagine_1202

Despotović J, Nikolić A. Metastatic colorectal cancer chemotherapeutic drugs alter the expression of TGF beta signaling-related miR-175p, miR-21-5p and miR-93-5p in vitro. in European Journal of Human Genetics. 2019;27:1581-1582.
https://hdl.handle.net/21.15107/rcub_imagine_1202 .

Despotović, Jovana, Nikolić, Aleksandra, "Metastatic colorectal cancer chemotherapeutic drugs alter the expression of TGF beta signaling-related miR-175p, miR-21-5p and miR-93-5p in vitro" in European Journal of Human Genetics, 27 (2019):1581-1582,
https://hdl.handle.net/21.15107/rcub_imagine_1202 .

TY  - JOUR
AU  - Kovač, Mirjana
AU  - Mitić, Gorana
AU  - Miković, Zeljko
AU  - Mandić, Vesna
AU  - Miljić, Predrag
AU  - Mitrović, Mirjana
AU  - Tomić, Branko
AU  - Bereczky, Zsuzsanna
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1287
AB  - Background: Inherited antithrombin (AT) deficiency is a rare autosomal dominant disorder, caused by mutations in the SERPINC1 gene. The most common clinical presentation in AT deficient patients includes venous thrombosis and pulmonary embolism, while the association of AT deficiency and its effect on the development of pregnancy complications has been less studied. The aim of our research was to evaluate the effect of AT deficiency types, determined by genotyping, on pregnancy outcomes. Methods: A retrospective cohort study included 28 women with AT deficiency, and their 64 pregnancies were analyzed. Results: With regard to live birth rate, a significant difference was observed among women who were carriers of different SERPINC1 mutations, as the rate varied from 100% in cases of type I to the extremely low rate of 8% for women with type II HBS (AT Budapest 3) in the homozygous variant, P=0.0005. All pregnancies from the type I group, even untreated ones, resulted in live births. In women with AT Budapest 3 in homozygous variant the overall live birth rate increased to 28.5% in the treated pregnancies. In this group the highest incidence of fetal death was observed of 62%; repeated fetal losses in 30%; fetal growth restriction in 22% and placental abruption in 7% of all pregnancies. Conclusion: Our study results indicate a difference between type I and type II AT deficiency. The risk of pregnancy related VTE was equally present in both groups, except for AT Budapest 3 in the heterozygous variant, while adverse pregnancy outcomes were strictly related to type II, especially AT Budapest 3 in the homozygous variant.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Thrombosis Research
T1  - The influence of specific mutations in the AT gene (SERPINC1) on the type of pregnancy related complications
EP  - 19
SP  - 12
VL  - 173
DO  - 10.1016/j.thromres.2018.11.006
ER  - 

@article{
author = "Kovač, Mirjana and Mitić, Gorana and Miković, Zeljko and Mandić, Vesna and Miljić, Predrag and Mitrović, Mirjana and Tomić, Branko and Bereczky, Zsuzsanna",
year = "2019",
abstract = "Background: Inherited antithrombin (AT) deficiency is a rare autosomal dominant disorder, caused by mutations in the SERPINC1 gene. The most common clinical presentation in AT deficient patients includes venous thrombosis and pulmonary embolism, while the association of AT deficiency and its effect on the development of pregnancy complications has been less studied. The aim of our research was to evaluate the effect of AT deficiency types, determined by genotyping, on pregnancy outcomes. Methods: A retrospective cohort study included 28 women with AT deficiency, and their 64 pregnancies were analyzed. Results: With regard to live birth rate, a significant difference was observed among women who were carriers of different SERPINC1 mutations, as the rate varied from 100% in cases of type I to the extremely low rate of 8% for women with type II HBS (AT Budapest 3) in the homozygous variant, P=0.0005. All pregnancies from the type I group, even untreated ones, resulted in live births. In women with AT Budapest 3 in homozygous variant the overall live birth rate increased to 28.5% in the treated pregnancies. In this group the highest incidence of fetal death was observed of 62%; repeated fetal losses in 30%; fetal growth restriction in 22% and placental abruption in 7% of all pregnancies. Conclusion: Our study results indicate a difference between type I and type II AT deficiency. The risk of pregnancy related VTE was equally present in both groups, except for AT Budapest 3 in the heterozygous variant, while adverse pregnancy outcomes were strictly related to type II, especially AT Budapest 3 in the homozygous variant.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Thrombosis Research",
title = "The influence of specific mutations in the AT gene (SERPINC1) on the type of pregnancy related complications",
pages = "19-12",
volume = "173",
doi = "10.1016/j.thromres.2018.11.006"
}

Kovač, M., Mitić, G., Miković, Z., Mandić, V., Miljić, P., Mitrović, M., Tomić, B.,& Bereczky, Z.. (2019). The influence of specific mutations in the AT gene (SERPINC1) on the type of pregnancy related complications. in Thrombosis Research
Pergamon-Elsevier Science Ltd, Oxford., 173, 12-19.
https://doi.org/10.1016/j.thromres.2018.11.006

Kovač M, Mitić G, Miković Z, Mandić V, Miljić P, Mitrović M, Tomić B, Bereczky Z. The influence of specific mutations in the AT gene (SERPINC1) on the type of pregnancy related complications. in Thrombosis Research. 2019;173:12-19.
doi:10.1016/j.thromres.2018.11.006 .

Kovač, Mirjana, Mitić, Gorana, Miković, Zeljko, Mandić, Vesna, Miljić, Predrag, Mitrović, Mirjana, Tomić, Branko, Bereczky, Zsuzsanna, "The influence of specific mutations in the AT gene (SERPINC1) on the type of pregnancy related complications" in Thrombosis Research, 173 (2019):12-19,
https://doi.org/10.1016/j.thromres.2018.11.006 . .

TY  - JOUR
AU  - Kovač, Mirjana
AU  - Mitić, Gorana
AU  - Đilas, Iva
AU  - Kuzmanović, Milos
AU  - Serbić, Olivera
AU  - Leković, Danijela
AU  - Tomić, Branko
AU  - Bereczky, Zsuzsanna
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1208
AB  - Inherited antithrombin (AT) deficiency is a rare autosomal dominant disorder, caused by mutations in the AT gene (SERPINC1). Considering that the genotype phenotype relationship in AT deficiency patients remains unclear, especially in pediatric patients, the aim of our study was to evaluate genotype phenotype correlation in a Serbian pediatric population. A retrospective cohort study included 19 children younger than 18 years, from 15 Serbian families, with newly diagnosed AT deficiency. In 21% of the recruited families, mutations affecting exon 4, 5, and 6 of the SERPINC1 gene that causes type I AT deficiency were detected. In the remaining families, the mutation in exon 2 causing type II HBS (AT Budapest 3) was found. Thrombosis events were observed in 1 (33%) of those with type I, 11 (85%) of those with AT Budapest 3 in the homozygous respectively, and 1(33%) in the heterozygous form. Recurrent thrombosis was observed only in AT Budapest 3 in the homozygous form, in 27% during initial treatment of the first thrombotic event. Abdominal venous thrombosis and arterial ischemic stroke, observed in almost half of the children from the group with AT Budapest 3 in the homozygous form, were unprovoked in all cases. Conclusion: Type II HBS (AT Budapest 3) in the homozygous form is a strong risk factor for arterial and venous thrombosis in pediatric patients.What is Known:center dot Inherited AT deficiency is a rare autosomal dominant disorder, caused by mutations in the SERPINC1gene.center dot The genotype phenotype correlation in AT deficiency patients remains unclear, especially in pediatric patients.What is New:center dot The genetic results for our paediatric population predominantly showed the presence of a single specific mutation in exon 2, that causes type II HBS deficiency (AT Budapest 3).center dot In this group thrombosis mostly occurred as unprovoked, in almost half of them as abdominal thrombosis or stroke with high incidence of recurrent thrombosis, in 27% during initial treatment.
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - Genotype phenotype correlation in a pediatric population with antithrombin deficiency
EP  - 1478
IS  - 10
SP  - 1471
VL  - 178
DO  - 10.1007/s00431-019-03433-5
ER  - 

@article{
author = "Kovač, Mirjana and Mitić, Gorana and Đilas, Iva and Kuzmanović, Milos and Serbić, Olivera and Leković, Danijela and Tomić, Branko and Bereczky, Zsuzsanna",
year = "2019",
abstract = "Inherited antithrombin (AT) deficiency is a rare autosomal dominant disorder, caused by mutations in the AT gene (SERPINC1). Considering that the genotype phenotype relationship in AT deficiency patients remains unclear, especially in pediatric patients, the aim of our study was to evaluate genotype phenotype correlation in a Serbian pediatric population. A retrospective cohort study included 19 children younger than 18 years, from 15 Serbian families, with newly diagnosed AT deficiency. In 21% of the recruited families, mutations affecting exon 4, 5, and 6 of the SERPINC1 gene that causes type I AT deficiency were detected. In the remaining families, the mutation in exon 2 causing type II HBS (AT Budapest 3) was found. Thrombosis events were observed in 1 (33%) of those with type I, 11 (85%) of those with AT Budapest 3 in the homozygous respectively, and 1(33%) in the heterozygous form. Recurrent thrombosis was observed only in AT Budapest 3 in the homozygous form, in 27% during initial treatment of the first thrombotic event. Abdominal venous thrombosis and arterial ischemic stroke, observed in almost half of the children from the group with AT Budapest 3 in the homozygous form, were unprovoked in all cases. Conclusion: Type II HBS (AT Budapest 3) in the homozygous form is a strong risk factor for arterial and venous thrombosis in pediatric patients.What is Known:center dot Inherited AT deficiency is a rare autosomal dominant disorder, caused by mutations in the SERPINC1gene.center dot The genotype phenotype correlation in AT deficiency patients remains unclear, especially in pediatric patients.What is New:center dot The genetic results for our paediatric population predominantly showed the presence of a single specific mutation in exon 2, that causes type II HBS deficiency (AT Budapest 3).center dot In this group thrombosis mostly occurred as unprovoked, in almost half of them as abdominal thrombosis or stroke with high incidence of recurrent thrombosis, in 27% during initial treatment.",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "Genotype phenotype correlation in a pediatric population with antithrombin deficiency",
pages = "1478-1471",
number = "10",
volume = "178",
doi = "10.1007/s00431-019-03433-5"
}

Kovač, M., Mitić, G., Đilas, I., Kuzmanović, M., Serbić, O., Leković, D., Tomić, B.,& Bereczky, Z.. (2019). Genotype phenotype correlation in a pediatric population with antithrombin deficiency. in European Journal of Pediatrics
Springer, New York., 178(10), 1471-1478.
https://doi.org/10.1007/s00431-019-03433-5

Kovač M, Mitić G, Đilas I, Kuzmanović M, Serbić O, Leković D, Tomić B, Bereczky Z. Genotype phenotype correlation in a pediatric population with antithrombin deficiency. in European Journal of Pediatrics. 2019;178(10):1471-1478.
doi:10.1007/s00431-019-03433-5 .

Kovač, Mirjana, Mitić, Gorana, Đilas, Iva, Kuzmanović, Milos, Serbić, Olivera, Leković, Danijela, Tomić, Branko, Bereczky, Zsuzsanna, "Genotype phenotype correlation in a pediatric population with antithrombin deficiency" in European Journal of Pediatrics, 178, no. 10 (2019):1471-1478,
https://doi.org/10.1007/s00431-019-03433-5 . .