TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Krstić, Aleksandar
AU  - Gradinac, Marija
AU  - Ilisić, Tamara
AU  - Parezanović, Vojislav
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Jovanović, Ida
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/678
AB  - Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients.
PB  - Cambridge Univ Press, Cambridge
T2  - Cardiology in the Young
T1  - The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2
EP  - 188
IS  - 2
SP  - 181
VL  - 23
DO  - 10.1017/S1047951112000571
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Krstić, Aleksandar and Gradinac, Marija and Ilisić, Tamara and Parezanović, Vojislav and Milivojević, Milena and Stevanović, Milena and Jovanović, Ida",
year = "2013",
abstract = "Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients.",
publisher = "Cambridge Univ Press, Cambridge",
journal = "Cardiology in the Young",
title = "The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2",
pages = "188-181",
number = "2",
volume = "23",
doi = "10.1017/S1047951112000571"
}

Cuturilo, G., Drakulić, D., Krstić, A., Gradinac, M., Ilisić, T., Parezanović, V., Milivojević, M., Stevanović, M.,& Jovanović, I.. (2013). The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2. in Cardiology in the Young
Cambridge Univ Press, Cambridge., 23(2), 181-188.
https://doi.org/10.1017/S1047951112000571

Cuturilo G, Drakulić D, Krstić A, Gradinac M, Ilisić T, Parezanović V, Milivojević M, Stevanović M, Jovanović I. The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2. in Cardiology in the Young. 2013;23(2):181-188.
doi:10.1017/S1047951112000571 .

Cuturilo, Goran, Drakulić, Danijela, Krstić, Aleksandar, Gradinac, Marija, Ilisić, Tamara, Parezanović, Vojislav, Milivojević, Milena, Stevanović, Milena, Jovanović, Ida, "The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2" in Cardiology in the Young, 23, no. 2 (2013):181-188,
https://doi.org/10.1017/S1047951112000571 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Popović, Jelena
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/585
AB  - SOX3 is a member of the Sox gene family implicated in brain formation and cognitive function. It is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Recently, we have established the first link between TALE (three-amino-acid loop extension) proteins, PBX1 (pre-B-cell leukemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue), and the expression of the human SOX3 gene. Here we present the evidence that TGIF (TG-interacting factor) is an additional TALE superfamily member involved in the regulation of human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus binding site that is conserved in primate orthologue promoters. Functional analysis demonstrated that mutation of the TGIF binding site resulted in the activation of SOX3 promoter. TGIF overexpression downregulates SOX3 promoter activity and decreases endogenous SOX3 protein expression in both uninduced and retinoic acid (RA)-induced NT2/D1 cells. Up to now, this is the first transcription factor identified as a negative regulator of SOX3 gene expression. The obtained results further underscore the significance of TALE proteins as important transcriptional regulators of SOX3 gene expression.
PB  - Science Press, Beijing
T2  - Journal of Genetics and Genomics
T1  - TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line
EP  - 27
IS  - 1
SP  - 19
VL  - 39
DO  - 10.1016/j.jgg.2011.11.006
ER  - 

@article{
author = "Mojsin, Marija and Popović, Jelena and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2012",
abstract = "SOX3 is a member of the Sox gene family implicated in brain formation and cognitive function. It is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Recently, we have established the first link between TALE (three-amino-acid loop extension) proteins, PBX1 (pre-B-cell leukemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue), and the expression of the human SOX3 gene. Here we present the evidence that TGIF (TG-interacting factor) is an additional TALE superfamily member involved in the regulation of human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus binding site that is conserved in primate orthologue promoters. Functional analysis demonstrated that mutation of the TGIF binding site resulted in the activation of SOX3 promoter. TGIF overexpression downregulates SOX3 promoter activity and decreases endogenous SOX3 protein expression in both uninduced and retinoic acid (RA)-induced NT2/D1 cells. Up to now, this is the first transcription factor identified as a negative regulator of SOX3 gene expression. The obtained results further underscore the significance of TALE proteins as important transcriptional regulators of SOX3 gene expression.",
publisher = "Science Press, Beijing",
journal = "Journal of Genetics and Genomics",
title = "TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line",
pages = "27-19",
number = "1",
volume = "39",
doi = "10.1016/j.jgg.2011.11.006"
}

Mojsin, M., Popović, J., Kovačević Grujičić, N.,& Stevanović, M.. (2012). TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line. in Journal of Genetics and Genomics
Science Press, Beijing., 39(1), 19-27.
https://doi.org/10.1016/j.jgg.2011.11.006

Mojsin M, Popović J, Kovačević Grujičić N, Stevanović M. TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line. in Journal of Genetics and Genomics. 2012;39(1):19-27.
doi:10.1016/j.jgg.2011.11.006 .

Mojsin, Marija, Popović, Jelena, Kovačević Grujičić, Nataša, Stevanović, Milena, "TG-interacting Factor (TGIF) Downregulates SOX3 Gene Expression in the NT2/D1 Cell Line" in Journal of Genetics and Genomics, 39, no. 1 (2012):19-27,
https://doi.org/10.1016/j.jgg.2011.11.006 . .

TY  - JOUR
AU  - Mikulak, Joanna
AU  - Negrini, Sara
AU  - Lazić, Andrijana
AU  - D'Alessandro, Rosalba
AU  - Mavilio, Domenico
AU  - Meldolesi, Jacopo
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/581
AB  - L1 cell adhesion molecule (L1CAM), an adhesion/signaling protein encoded by a gene target of the transcription repressor RE-1-Silencing Transcription factor (REST), is expressed in two alternatively spliced isoforms. The full-length isoform, typical of low-REST neural cells, plays key roles in survival/migration, outgrowth/fasciculation/regeneration of axons, synaptic plasticity; the isoform missing two mini-exons, abundant in a few high-REST non-neural cells, maintains some effect on migration and proliferation. To investigate whether and how L1CAM alternative splicing depends on REST we used neural cell models expressing low or high levels of REST (PC12, SH-SY5Y, differentiated NT2/D1 and primary neurons transduced or not with REST). The short isoform was found to rise when the low-REST levels of neural cells were experimentally increased, while the full-length isoform increased in high-REST cells when the repressor tone was attenuated. These results were due to Nova2, a neural cell-specific splicing factor shown here to be repressed by REST. REST control of L1CAM occurs therefore by two mechanisms, transcription and alternative splicing. The splicing mechanism, affecting not only L1CAM but all Nova2 targets (similar to 7% of brain-specific splicing, including the mRNAs of other adhesion and synaptic proteins) is expected to be critical during development and important also for the structure and function of mature neural cells.
PB  - Wiley, Hoboken
T2  - Journal of Neurochemistry
T1  - Dual REST-dependence of L1CAM: from gene expression to alternative splicing governed by Nova2 in neural cells
EP  - 709
IS  - 5
SP  - 699
VL  - 120
DO  - 10.1111/j.1471-4159.2011.07626.x
ER  - 

@article{
author = "Mikulak, Joanna and Negrini, Sara and Lazić, Andrijana and D'Alessandro, Rosalba and Mavilio, Domenico and Meldolesi, Jacopo",
year = "2012",
abstract = "L1 cell adhesion molecule (L1CAM), an adhesion/signaling protein encoded by a gene target of the transcription repressor RE-1-Silencing Transcription factor (REST), is expressed in two alternatively spliced isoforms. The full-length isoform, typical of low-REST neural cells, plays key roles in survival/migration, outgrowth/fasciculation/regeneration of axons, synaptic plasticity; the isoform missing two mini-exons, abundant in a few high-REST non-neural cells, maintains some effect on migration and proliferation. To investigate whether and how L1CAM alternative splicing depends on REST we used neural cell models expressing low or high levels of REST (PC12, SH-SY5Y, differentiated NT2/D1 and primary neurons transduced or not with REST). The short isoform was found to rise when the low-REST levels of neural cells were experimentally increased, while the full-length isoform increased in high-REST cells when the repressor tone was attenuated. These results were due to Nova2, a neural cell-specific splicing factor shown here to be repressed by REST. REST control of L1CAM occurs therefore by two mechanisms, transcription and alternative splicing. The splicing mechanism, affecting not only L1CAM but all Nova2 targets (similar to 7% of brain-specific splicing, including the mRNAs of other adhesion and synaptic proteins) is expected to be critical during development and important also for the structure and function of mature neural cells.",
publisher = "Wiley, Hoboken",
journal = "Journal of Neurochemistry",
title = "Dual REST-dependence of L1CAM: from gene expression to alternative splicing governed by Nova2 in neural cells",
pages = "709-699",
number = "5",
volume = "120",
doi = "10.1111/j.1471-4159.2011.07626.x"
}

Mikulak, J., Negrini, S., Lazić, A., D'Alessandro, R., Mavilio, D.,& Meldolesi, J.. (2012). Dual REST-dependence of L1CAM: from gene expression to alternative splicing governed by Nova2 in neural cells. in Journal of Neurochemistry
Wiley, Hoboken., 120(5), 699-709.
https://doi.org/10.1111/j.1471-4159.2011.07626.x

Mikulak J, Negrini S, Lazić A, D'Alessandro R, Mavilio D, Meldolesi J. Dual REST-dependence of L1CAM: from gene expression to alternative splicing governed by Nova2 in neural cells. in Journal of Neurochemistry. 2012;120(5):699-709.
doi:10.1111/j.1471-4159.2011.07626.x .

Mikulak, Joanna, Negrini, Sara, Lazić, Andrijana, D'Alessandro, Rosalba, Mavilio, Domenico, Meldolesi, Jacopo, "Dual REST-dependence of L1CAM: from gene expression to alternative splicing governed by Nova2 in neural cells" in Journal of Neurochemistry, 120, no. 5 (2012):699-709,
https://doi.org/10.1111/j.1471-4159.2011.07626.x . .

TY  - JOUR
AU  - Drakulić, Danijela
AU  - Krstić, A.
AU  - Stevanović, Milena
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/561
AB  - SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and characterized SOX2-overexpressing NT2/D1 cell clones. Using Southern blot and semi-quantitative RT-PCR, we confirmed integration and expression of exogenous SOX2 in three NT2/D1 cell clones. Overexpression of the SOX2 gene was detected in two of these clones. SOX2 overexpression in NT2/D1 cell clones resulted in altered expression of key pluripotency genes OCT4 and NANOG. Furthermore, SOX2-overexpressing NT2/D1 cell clones entered into retinoic acid-dependent neural differentiation, even when there was elevated SOX2 expression. After 21 days of induction by retinoic acid, expression of neural markers (neuroD1 and synaptophysin) was higher in induced cell clones than in induced parental cells. The cell clone with SOX2 overexpression had an approximately 1.3-fold higher growth rate compared to parental cells. SOX2 overexpression did not increase the population of cells undergoing apoptosis. Taken together, we developed two SOX2-overexpressing cell clones, with constitutive SOX2 expression after three weeks of retinoic acid treatment. SOX2 overexpression resulted in altered expression of pluripotency-related genes, increased proliferation, and altered expression of neural markers after three weeks of retinoic acid treatment.
PB  - FUNPEC-Editora, Ribeirao Preto
T2  - Genetics and Molecular Research
T1  - Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones
EP  - 1400
IS  - 2
SP  - 1385
VL  - 11
DO  - 10.4238/2012.May.15.9
ER  - 

@article{
author = "Drakulić, Danijela and Krstić, A. and Stevanović, Milena",
year = "2012",
abstract = "SOX2, a universal marker of pluripotent stem cells, is a transcription factor that helps control embryonic development in vertebrates; its expression persists in neural stem/progenitor cells into adulthood. Considering the critical role of the SOX2 transcription factor in the regulation of genes required for self-renewal and pluripotency of stem cells, we developed and characterized SOX2-overexpressing NT2/D1 cell clones. Using Southern blot and semi-quantitative RT-PCR, we confirmed integration and expression of exogenous SOX2 in three NT2/D1 cell clones. Overexpression of the SOX2 gene was detected in two of these clones. SOX2 overexpression in NT2/D1 cell clones resulted in altered expression of key pluripotency genes OCT4 and NANOG. Furthermore, SOX2-overexpressing NT2/D1 cell clones entered into retinoic acid-dependent neural differentiation, even when there was elevated SOX2 expression. After 21 days of induction by retinoic acid, expression of neural markers (neuroD1 and synaptophysin) was higher in induced cell clones than in induced parental cells. The cell clone with SOX2 overexpression had an approximately 1.3-fold higher growth rate compared to parental cells. SOX2 overexpression did not increase the population of cells undergoing apoptosis. Taken together, we developed two SOX2-overexpressing cell clones, with constitutive SOX2 expression after three weeks of retinoic acid treatment. SOX2 overexpression resulted in altered expression of pluripotency-related genes, increased proliferation, and altered expression of neural markers after three weeks of retinoic acid treatment.",
publisher = "FUNPEC-Editora, Ribeirao Preto",
journal = "Genetics and Molecular Research",
title = "Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones",
pages = "1400-1385",
number = "2",
volume = "11",
doi = "10.4238/2012.May.15.9"
}

Drakulić, D., Krstić, A.,& Stevanović, M.. (2012). Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones. in Genetics and Molecular Research
FUNPEC-Editora, Ribeirao Preto., 11(2), 1385-1400.
https://doi.org/10.4238/2012.May.15.9

Drakulić D, Krstić A, Stevanović M. Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones. in Genetics and Molecular Research. 2012;11(2):1385-1400.
doi:10.4238/2012.May.15.9 .

Drakulić, Danijela, Krstić, A., Stevanović, Milena, "Establishment and initial characterization of SOX2-overexpressing NT2/D1 cell clones" in Genetics and Molecular Research, 11, no. 2 (2012):1385-1400,
https://doi.org/10.4238/2012.May.15.9 . .

TY  - JOUR
AU  - Nikčević, Gordana
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Krstić, A.
AU  - Savić, T.
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/485
AB  - Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates. Despite the mounting evidence that Sox3/SOX3 is one of the key players in the development of the nervous system, limited data are available regarding the transcriptional regulation of its expression. This review is focused on the retinoic acid induced regulation of SOX3 gene expression, with particular emphasis on the involvement of retinoid receptors. Experiments with human embryonal carcinoma cells identified two response elements involved in retinoic acid/retinoid X receptor-dependent activation of the SOX3 gene expression: distal atypical retinoic acid-response element, consisting of two unique G-rich boxes separated by 49 bp, and proximal element comprising DR-3-like motif, composed of two imperfect hexameric half-sites. Importantly, the retinoic acid-induced SOX3 gene expression could be significantly down-regulated by a synthetic antagonist of retinoid receptors. This cell model provides a solid base for further studies on mechanism(s) underlying regulation of expression of SOX3 gene, which could improve the understanding of molecular signals that induce neurogenesis in the stem/progenitor cells both during development and in adulthood.
PB  - Acad Sciences Czech Republic, Inst Physiology, Prague 4
T2  - Physiological Research
T1  - Regulation of the SOX3 Gene Expression by Retinoid Receptors
EP  - S91
SP  - S83
VL  - 60
DO  - 10.33549/physiolres.932184
ER  - 

@article{
author = "Nikčević, Gordana and Kovačević Grujičić, Nataša and Mojsin, Marija and Krstić, A. and Savić, T. and Stevanović, Milena",
year = "2011",
abstract = "Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates. Despite the mounting evidence that Sox3/SOX3 is one of the key players in the development of the nervous system, limited data are available regarding the transcriptional regulation of its expression. This review is focused on the retinoic acid induced regulation of SOX3 gene expression, with particular emphasis on the involvement of retinoid receptors. Experiments with human embryonal carcinoma cells identified two response elements involved in retinoic acid/retinoid X receptor-dependent activation of the SOX3 gene expression: distal atypical retinoic acid-response element, consisting of two unique G-rich boxes separated by 49 bp, and proximal element comprising DR-3-like motif, composed of two imperfect hexameric half-sites. Importantly, the retinoic acid-induced SOX3 gene expression could be significantly down-regulated by a synthetic antagonist of retinoid receptors. This cell model provides a solid base for further studies on mechanism(s) underlying regulation of expression of SOX3 gene, which could improve the understanding of molecular signals that induce neurogenesis in the stem/progenitor cells both during development and in adulthood.",
publisher = "Acad Sciences Czech Republic, Inst Physiology, Prague 4",
journal = "Physiological Research",
title = "Regulation of the SOX3 Gene Expression by Retinoid Receptors",
pages = "S91-S83",
volume = "60",
doi = "10.33549/physiolres.932184"
}

Nikčević, G., Kovačević Grujičić, N., Mojsin, M., Krstić, A., Savić, T.,& Stevanović, M.. (2011). Regulation of the SOX3 Gene Expression by Retinoid Receptors. in Physiological Research
Acad Sciences Czech Republic, Inst Physiology, Prague 4., 60, S83-S91.
https://doi.org/10.33549/physiolres.932184

Nikčević G, Kovačević Grujičić N, Mojsin M, Krstić A, Savić T, Stevanović M. Regulation of the SOX3 Gene Expression by Retinoid Receptors. in Physiological Research. 2011;60:S83-S91.
doi:10.33549/physiolres.932184 .

Nikčević, Gordana, Kovačević Grujičić, Nataša, Mojsin, Marija, Krstić, A., Savić, T., Stevanović, Milena, "Regulation of the SOX3 Gene Expression by Retinoid Receptors" in Physiological Research, 60 (2011):S83-S91,
https://doi.org/10.33549/physiolres.932184 . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Menten, Bjorn
AU  - Krstić, Aleksandar
AU  - Drakulić, Danijela
AU  - Jovanović, Ida
AU  - Parezanović, Vojislav
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/494
AB  - Small terminal or interstitial deletions involving bands 4q34 and 4q35 have been described in several patients with a relatively mild phenotype such as mild to moderate intellectual disability and minor dysmorphic features. We present a boy born from unrelated parents with a de novo 4q34.1-q35.2 deletion and clinical features resembling 22q11.2 deletion syndrome. To the best of our knowledge, this is the first reported patient with 4q34-q35 deletion and phenotype resembling 22q11.2 deletion syndrome without fifth finger anomalies as a specific feature of 4q- syndrome. G-banding karyotyping disclosed the deletion, which was further delineated by microarray comparative genomic hybridization. Fluorescence in situ hybridization and multiplex ligation-dependent probe amplification analyses did not reveal rearrangements of 22q11.2 region. MLPA confirmed the deletion within the 4q35.2 region. Conclusion: Given the considerable clinical overlaps between the 22q11.2 deletion syndrome and clinical manifestation of the patient described in this study, we propose that region 4q34.1-q35.2 should be considered as another region associated with phenotype resembling 22q11.2 deletion syndrome. We also propose that distal 4q deletions should be considered in the evaluation of patients with phenotypic manifestations resembling 22q11.2 deletion syndrome in whom no 22q11.2 micro-deletion was detected, even in the absence of distinctive fifth finger anomalies. Additionally, we underline the importance of applying array CGH that enables simultaneous genome-wide detection and delineation of copy number changes (e. g., deletions and duplications).
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome
EP  - 1470
IS  - 11
SP  - 1465
VL  - 170
DO  - 10.1007/s00431-011-1533-3
ER  - 

@article{
author = "Cuturilo, Goran and Menten, Bjorn and Krstić, Aleksandar and Drakulić, Danijela and Jovanović, Ida and Parezanović, Vojislav and Stevanović, Milena",
year = "2011",
abstract = "Small terminal or interstitial deletions involving bands 4q34 and 4q35 have been described in several patients with a relatively mild phenotype such as mild to moderate intellectual disability and minor dysmorphic features. We present a boy born from unrelated parents with a de novo 4q34.1-q35.2 deletion and clinical features resembling 22q11.2 deletion syndrome. To the best of our knowledge, this is the first reported patient with 4q34-q35 deletion and phenotype resembling 22q11.2 deletion syndrome without fifth finger anomalies as a specific feature of 4q- syndrome. G-banding karyotyping disclosed the deletion, which was further delineated by microarray comparative genomic hybridization. Fluorescence in situ hybridization and multiplex ligation-dependent probe amplification analyses did not reveal rearrangements of 22q11.2 region. MLPA confirmed the deletion within the 4q35.2 region. Conclusion: Given the considerable clinical overlaps between the 22q11.2 deletion syndrome and clinical manifestation of the patient described in this study, we propose that region 4q34.1-q35.2 should be considered as another region associated with phenotype resembling 22q11.2 deletion syndrome. We also propose that distal 4q deletions should be considered in the evaluation of patients with phenotypic manifestations resembling 22q11.2 deletion syndrome in whom no 22q11.2 micro-deletion was detected, even in the absence of distinctive fifth finger anomalies. Additionally, we underline the importance of applying array CGH that enables simultaneous genome-wide detection and delineation of copy number changes (e. g., deletions and duplications).",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome",
pages = "1470-1465",
number = "11",
volume = "170",
doi = "10.1007/s00431-011-1533-3"
}

Cuturilo, G., Menten, B., Krstić, A., Drakulić, D., Jovanović, I., Parezanović, V.,& Stevanović, M.. (2011). 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome. in European Journal of Pediatrics
Springer, New York., 170(11), 1465-1470.
https://doi.org/10.1007/s00431-011-1533-3

Cuturilo G, Menten B, Krstić A, Drakulić D, Jovanović I, Parezanović V, Stevanović M. 4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome. in European Journal of Pediatrics. 2011;170(11):1465-1470.
doi:10.1007/s00431-011-1533-3 .

Cuturilo, Goran, Menten, Bjorn, Krstić, Aleksandar, Drakulić, Danijela, Jovanović, Ida, Parezanović, Vojislav, Stevanović, Milena, "4q34.1-q35.2 deletion in a boy with phenotype resembling 22q11.2 deletion syndrome" in European Journal of Pediatrics, 170, no. 11 (2011):1465-1470,
https://doi.org/10.1007/s00431-011-1533-3 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Popović, Jelena
AU  - Krstić, A.
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/526
AB  - The SOX18 transcription factor plays an important role in endothelial cell specification, angiogenesis and atherogenesis. By profiling transcription factor interactions (TranSignal TM TF Protein Array) we identified several transcription factors implicated in angiogenesis that have the ability to bind to the SOX18 optimal promoter region in vitro. In this report we focused our attention on distinct transcription factors identified by the array as belonging to AP-1 and CREB/ATF protein families. In particular, we analyzed the effects of CREB, JunB, c-Jun and ATF3 on SOX18 gene expression. Functional analysis revealed that CREB acts as a repressor, while JunB, c-Jun and ATF3 act as activators of SOX18 promoter activity. Our findings indicate that a transcriptional network that includes CREB, JunB, c-Jun and ATF3 could be involved in angiogenesis-related transcriptional regulation of the SOX18 gene.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression
EP  - 525
IS  - 3
SP  - 517
VL  - 63
DO  - 10.2298/ABS1103517P
ER  - 

@article{
author = "Petrović, Isidora and Kovačević Grujičić, Nataša and Popović, Jelena and Krstić, A. and Milivojević, Milena and Stevanović, Milena",
year = "2011",
abstract = "The SOX18 transcription factor plays an important role in endothelial cell specification, angiogenesis and atherogenesis. By profiling transcription factor interactions (TranSignal TM TF Protein Array) we identified several transcription factors implicated in angiogenesis that have the ability to bind to the SOX18 optimal promoter region in vitro. In this report we focused our attention on distinct transcription factors identified by the array as belonging to AP-1 and CREB/ATF protein families. In particular, we analyzed the effects of CREB, JunB, c-Jun and ATF3 on SOX18 gene expression. Functional analysis revealed that CREB acts as a repressor, while JunB, c-Jun and ATF3 act as activators of SOX18 promoter activity. Our findings indicate that a transcriptional network that includes CREB, JunB, c-Jun and ATF3 could be involved in angiogenesis-related transcriptional regulation of the SOX18 gene.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression",
pages = "525-517",
number = "3",
volume = "63",
doi = "10.2298/ABS1103517P"
}

Petrović, I., Kovačević Grujičić, N., Popović, J., Krstić, A., Milivojević, M.,& Stevanović, M.. (2011). Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 63(3), 517-525.
https://doi.org/10.2298/ABS1103517P

Petrović I, Kovačević Grujičić N, Popović J, Krstić A, Milivojević M, Stevanović M. Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression. in Archives of Biological Sciences. 2011;63(3):517-525.
doi:10.2298/ABS1103517P .

Petrović, Isidora, Kovačević Grujičić, Nataša, Popović, Jelena, Krstić, A., Milivojević, Milena, Stevanović, Milena, "Members of the CREB/ATF and AP1 family of transcription factors are involved in the regulation of SOX18 gene expression" in Archives of Biological Sciences, 63, no. 3 (2011):517-525,
https://doi.org/10.2298/ABS1103517P . .

TY  - JOUR
AU  - Tomasoni, Romana
AU  - Negrini, Sara
AU  - Fiordaliso, Stefania
AU  - Lazić, Andrijana
AU  - Tkatch, Tatiana
AU  - Mondino, Anna
AU  - Meldolesi, Jacopo
AU  - D'Alessandro, Rosalba
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/480
AB  - The RE-1-specific silencing transcription factor (REST or NRSF) is a transcription repressor that orchestrates differentiation and also operates in differentiated neurons and neurosecretory cells (neural cells). Its role in proliferation has been investigated so far only in rapidly growing tumors, with conflicting results: suppression in non-neural tumors, stimulation in medulloblastomas. Working with two clones of chromaffin-neuronal PC12 cells, which express different levels of REST, and using genetic complementation and knockdown approaches, we show that REST also promotes proliferation in differentiated neural cells. Mechanistically, this occurs by a signaling pathway involving REST, the GTPase-activating protein tuberin (TSC2) and the transcription co-factor beta-catenin. In PC12 cells, raised expression of REST correlates with reduced TSC2 levels, nuclear accumulation and co-transcriptional activation of beta-catenin, and increased expression of its target oncogenes Myc and Ccnd1, which might account for the proliferation advantage and the distinct morphology. Rest transcription is also increased, unveiling the existence of a self-sustaining, feed-forward REST-TSC2-beta-catenin signaling loop that is also operative in another neural cell model, NT2/D1 cells. Transfection of REST, knockdown of TSC2 or forced expression of active beta-catenin recapitulated the biochemical, functional and morphological properties of the high-expressing REST clone in wild-type PC12 cells. Upregulation of REST promoted proliferation and phenotypic changes, thus hindering neurosecretion. The new REST-TSC2-beta-catenin signaling paradigm might have an important role in various aspects of neural cell physiology and pathology, including the regulation of proliferation and neurosecretion.
PB  - Company Biologists Ltd, Cambridge
T2  - Journal of Cell Science
T1  - A signaling loop of REST, TSC2 and beta-catenin governs proliferation and function of PC12 neural cells
EP  - 3186
IS  - 18
SP  - 3174
VL  - 124
DO  - 10.1242/jcs.087551
ER  - 

@article{
author = "Tomasoni, Romana and Negrini, Sara and Fiordaliso, Stefania and Lazić, Andrijana and Tkatch, Tatiana and Mondino, Anna and Meldolesi, Jacopo and D'Alessandro, Rosalba",
year = "2011",
abstract = "The RE-1-specific silencing transcription factor (REST or NRSF) is a transcription repressor that orchestrates differentiation and also operates in differentiated neurons and neurosecretory cells (neural cells). Its role in proliferation has been investigated so far only in rapidly growing tumors, with conflicting results: suppression in non-neural tumors, stimulation in medulloblastomas. Working with two clones of chromaffin-neuronal PC12 cells, which express different levels of REST, and using genetic complementation and knockdown approaches, we show that REST also promotes proliferation in differentiated neural cells. Mechanistically, this occurs by a signaling pathway involving REST, the GTPase-activating protein tuberin (TSC2) and the transcription co-factor beta-catenin. In PC12 cells, raised expression of REST correlates with reduced TSC2 levels, nuclear accumulation and co-transcriptional activation of beta-catenin, and increased expression of its target oncogenes Myc and Ccnd1, which might account for the proliferation advantage and the distinct morphology. Rest transcription is also increased, unveiling the existence of a self-sustaining, feed-forward REST-TSC2-beta-catenin signaling loop that is also operative in another neural cell model, NT2/D1 cells. Transfection of REST, knockdown of TSC2 or forced expression of active beta-catenin recapitulated the biochemical, functional and morphological properties of the high-expressing REST clone in wild-type PC12 cells. Upregulation of REST promoted proliferation and phenotypic changes, thus hindering neurosecretion. The new REST-TSC2-beta-catenin signaling paradigm might have an important role in various aspects of neural cell physiology and pathology, including the regulation of proliferation and neurosecretion.",
publisher = "Company Biologists Ltd, Cambridge",
journal = "Journal of Cell Science",
title = "A signaling loop of REST, TSC2 and beta-catenin governs proliferation and function of PC12 neural cells",
pages = "3186-3174",
number = "18",
volume = "124",
doi = "10.1242/jcs.087551"
}

Tomasoni, R., Negrini, S., Fiordaliso, S., Lazić, A., Tkatch, T., Mondino, A., Meldolesi, J.,& D'Alessandro, R.. (2011). A signaling loop of REST, TSC2 and beta-catenin governs proliferation and function of PC12 neural cells. in Journal of Cell Science
Company Biologists Ltd, Cambridge., 124(18), 3174-3186.
https://doi.org/10.1242/jcs.087551

Tomasoni R, Negrini S, Fiordaliso S, Lazić A, Tkatch T, Mondino A, Meldolesi J, D'Alessandro R. A signaling loop of REST, TSC2 and beta-catenin governs proliferation and function of PC12 neural cells. in Journal of Cell Science. 2011;124(18):3174-3186.
doi:10.1242/jcs.087551 .

Tomasoni, Romana, Negrini, Sara, Fiordaliso, Stefania, Lazić, Andrijana, Tkatch, Tatiana, Mondino, Anna, Meldolesi, Jacopo, D'Alessandro, Rosalba, "A signaling loop of REST, TSC2 and beta-catenin governs proliferation and function of PC12 neural cells" in Journal of Cell Science, 124, no. 18 (2011):3174-3186,
https://doi.org/10.1242/jcs.087551 . .

TY  - JOUR
AU  - Jovicić, Dubravka
AU  - Milacić, Snežana
AU  - Vukov, Tanja D.
AU  - Rakić, Boban
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Rakić, Rada
AU  - Bukvić, Nenad
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/406
AB  - We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization ( FISH). The latter was applied particularly in order to verify PCD in a specific chromosome ( chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p  lt  0.001) in frequencies between the two groups ( exposed and controls) for all the observed variables ( CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation. Health Phys. 98(5):717-726; 2010
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Health Physics
T1  - Detection of premature segregation of centromeres in persons exposed to ionizing radiation
EP  - 726
IS  - 5
SP  - 717
VL  - 98
DO  - 10.1097/HP.0b013e3181d26da1
ER  - 

@article{
author = "Jovicić, Dubravka and Milacić, Snežana and Vukov, Tanja D. and Rakić, Boban and Stevanović, Milena and Drakulić, Danijela and Rakić, Rada and Bukvić, Nenad",
year = "2010",
abstract = "We have analyzed the frequency of premature centromeric division (PCD) in medical personnel professionally exposed to low doses of radiation. They had chromosome aberrations (CAs) involving dicentric chromosomes, ring chromosomes, acentric fragments, chromosome breaks, and chromatid breaks. The study included 30 exposed subjects and 23 controls who were each analyzed by a conventional cytogenetics procedure and subsequently by fluorescent in situ hybridization ( FISH). The latter was applied particularly in order to verify PCD in a specific chromosome ( chromosome 18) in both metaphases and interphase nuclei. The results revealed a significant difference (p  lt  0.001) in frequencies between the two groups ( exposed and controls) for all the observed variables ( CAs), metaphases with PCD (MPCD), total number of chromosomes with PCD (TPCD), number of PCD metaphases in acrocentric chromosomes (MAPCD), and the total number of acrocentric chromosomes with PCD (TAPCD). The doses of ionizing radiation absorbed by the subjects' bodies were measured with thermoluminescent dosimeters once a month during the duration of occupational exposure. They were expressed in mSv, as mean annual effective doses for the period of exposure. The Spearman rank test showed a high positive correlation between total life effective dose and frequency of CAs and PCD. Based on the results obtained in this study, we suggest that PCD, as a phenomenon manifesting chromosomal instability (CIN), should be considered as a suitable cytogenetic biomarker for individuals occupationally exposed to ionizing radiation. Health Phys. 98(5):717-726; 2010",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Health Physics",
title = "Detection of premature segregation of centromeres in persons exposed to ionizing radiation",
pages = "726-717",
number = "5",
volume = "98",
doi = "10.1097/HP.0b013e3181d26da1"
}

Jovicić, D., Milacić, S., Vukov, T. D., Rakić, B., Stevanović, M., Drakulić, D., Rakić, R.,& Bukvić, N.. (2010). Detection of premature segregation of centromeres in persons exposed to ionizing radiation. in Health Physics
Lippincott Williams & Wilkins, Philadelphia., 98(5), 717-726.
https://doi.org/10.1097/HP.0b013e3181d26da1

Jovicić D, Milacić S, Vukov TD, Rakić B, Stevanović M, Drakulić D, Rakić R, Bukvić N. Detection of premature segregation of centromeres in persons exposed to ionizing radiation. in Health Physics. 2010;98(5):717-726.
doi:10.1097/HP.0b013e3181d26da1 .

Jovicić, Dubravka, Milacić, Snežana, Vukov, Tanja D., Rakić, Boban, Stevanović, Milena, Drakulić, Danijela, Rakić, Rada, Bukvić, Nenad, "Detection of premature segregation of centromeres in persons exposed to ionizing radiation" in Health Physics, 98, no. 5 (2010):717-726,
https://doi.org/10.1097/HP.0b013e3181d26da1 . .

TY  - JOUR
AU  - Đorđević, V. A.
AU  - Jovanović, J. V.
AU  - Pavković-Lucić, S. B.
AU  - Drakulić, Danijela
AU  - Đurović, M. M.
AU  - Gotić, M. D.
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/463
AB  - Cytogenetic findings are reported for 31 female patients with Turner's syndrome. Chromosome studies were made from lymphocyte cultures. Non-mosaicism 45, X was demonstrated in 15 of these patients, whereas only three were apparently mosaic. Eight patients showed non-mosaic and four patients showed mosaic structural aberrations of the X-chromosome. One non-mosaic case displayed a karyotype containing a small marker chromosome. Conventional cytogenetics was supplemented by fluorescence in situ hybridization (FISH) with an X-specific probe to identify the chromosomal origin of the ring and a 1q12-specific DNA probe to identify de novo balanced translocation (1;9) in one patient. To our knowledge, this is the first finding of karyotype 45,X,t(1;9) (cen;cen)/46,X,r(X),t(1;9)(cen;cen) in Turner's syndrome. The same X-specific probe was also used to identify a derivative chromosome in one patient.
PB  - FUNPEC-Editora, Ribeirao Preto
T2  - Genetics and Molecular Research
T1  - Cytogenetic findings in Serbian patients with Turner's syndrome stigmata
EP  - 2221
IS  - 4
SP  - 2213
VL  - 9
DO  - 10.4238/vol9-4gmr953
ER  - 

@article{
author = "Đorđević, V. A. and Jovanović, J. V. and Pavković-Lucić, S. B. and Drakulić, Danijela and Đurović, M. M. and Gotić, M. D.",
year = "2010",
abstract = "Cytogenetic findings are reported for 31 female patients with Turner's syndrome. Chromosome studies were made from lymphocyte cultures. Non-mosaicism 45, X was demonstrated in 15 of these patients, whereas only three were apparently mosaic. Eight patients showed non-mosaic and four patients showed mosaic structural aberrations of the X-chromosome. One non-mosaic case displayed a karyotype containing a small marker chromosome. Conventional cytogenetics was supplemented by fluorescence in situ hybridization (FISH) with an X-specific probe to identify the chromosomal origin of the ring and a 1q12-specific DNA probe to identify de novo balanced translocation (1;9) in one patient. To our knowledge, this is the first finding of karyotype 45,X,t(1;9) (cen;cen)/46,X,r(X),t(1;9)(cen;cen) in Turner's syndrome. The same X-specific probe was also used to identify a derivative chromosome in one patient.",
publisher = "FUNPEC-Editora, Ribeirao Preto",
journal = "Genetics and Molecular Research",
title = "Cytogenetic findings in Serbian patients with Turner's syndrome stigmata",
pages = "2221-2213",
number = "4",
volume = "9",
doi = "10.4238/vol9-4gmr953"
}

Đorđević, V. A., Jovanović, J. V., Pavković-Lucić, S. B., Drakulić, D., Đurović, M. M.,& Gotić, M. D.. (2010). Cytogenetic findings in Serbian patients with Turner's syndrome stigmata. in Genetics and Molecular Research
FUNPEC-Editora, Ribeirao Preto., 9(4), 2213-2221.
https://doi.org/10.4238/vol9-4gmr953

Đorđević VA, Jovanović JV, Pavković-Lucić SB, Drakulić D, Đurović MM, Gotić MD. Cytogenetic findings in Serbian patients with Turner's syndrome stigmata. in Genetics and Molecular Research. 2010;9(4):2213-2221.
doi:10.4238/vol9-4gmr953 .

Đorđević, V. A., Jovanović, J. V., Pavković-Lucić, S. B., Drakulić, Danijela, Đurović, M. M., Gotić, M. D., "Cytogenetic findings in Serbian patients with Turner's syndrome stigmata" in Genetics and Molecular Research, 9, no. 4 (2010):2213-2221,
https://doi.org/10.4238/vol9-4gmr953 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Krstić, Aleksandar
AU  - Popović, Jelena
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/407
AB  - To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.
PB  - Springer/Plenum Publishers, New York
T2  - Biochemical Genetics
T1  - Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution
EP  - 623
IS  - 7-8
SP  - 612
VL  - 48
DO  - 10.1007/s10528-010-9343-2
ER  - 

@article{
author = "Mojsin, Marija and Kovačević Grujičić, Nataša and Krstić, Aleksandar and Popović, Jelena and Milivojević, Milena and Stevanović, Milena",
year = "2010",
abstract = "To understand more fully the structure and evolution of the SOX3 protein, we comparatively analyzed its orthologs in vertebrates. Since complex disorders are associated with human SOX3 polyalanine expansions, our investigation focused on both compositional and evolutionary analysis of various homopolymeric amino acid tracts observed in SOX3 orthologs. Our analysis revealed that the observed homopolymeric alanine, glycine, and proline tracts are mammal-specific, except for one polyglycine tract present in birds. Since it is likely that the SOX3 protein acquired additional roles in brain development in Eutheria, we might speculate that development of novel brain functions during the course of evolution was affected, at least in part, by such structural-functional changes in the SOX3 protein.",
publisher = "Springer/Plenum Publishers, New York",
journal = "Biochemical Genetics",
title = "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution",
pages = "623-612",
number = "7-8",
volume = "48",
doi = "10.1007/s10528-010-9343-2"
}

Mojsin, M., Kovačević Grujičić, N., Krstić, A., Popović, J., Milivojević, M.,& Stevanović, M.. (2010). Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics
Springer/Plenum Publishers, New York., 48(7-8), 612-623.
https://doi.org/10.1007/s10528-010-9343-2

Mojsin M, Kovačević Grujičić N, Krstić A, Popović J, Milivojević M, Stevanović M. Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution. in Biochemical Genetics. 2010;48(7-8):612-623.
doi:10.1007/s10528-010-9343-2 .

Mojsin, Marija, Kovačević Grujičić, Nataša, Krstić, Aleksandar, Popović, Jelena, Milivojević, Milena, Stevanović, Milena, "Comparative Analysis of SOX3 Protein Orthologs: Expansion of Homopolymeric Amino Acid Tracts During Vertebrate Evolution" in Biochemical Genetics, 48, no. 7-8 (2010):612-623,
https://doi.org/10.1007/s10528-010-9343-2 . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/415
AB  - Sex-determining region Y box 18 (Sox18/SOX18) gene is an important regulator of vascular development playing a role in endothelial cell specification or differentiation, angiogenesis and atherogenesis. The aim of this study was to perform comprehensive functional characterization of the human SOX18 promoter, including determination of transcription start point (tsp) and identification of control elements involved in the regulation of SOX18 gene expression, with an emphasis on angiogenesis-related transcription factors. Analyses were performed in HeLa cells, representing a tumor cell line, and in EA.hy926 cells used as an endothelial model system. We have determined unique tsp of SOX18 gene, located 172 nucleotides upstream from ATG codon. Further, we have shown that SOX18 promoter region, -726 to -89 by relative to tsp, contains positive cis-regulatory element(s) that stimulates SOX18 promoter activity, while region -89 to + 166 represents the minimal promoter. Within this region we have recognized the presence of essential element(s), positioned from -89 to +29, which harbors cluster of three putative early growth response 1 (EGR1) binding sites. By in vitro binding assays and functional analyses we have shown that these three putative binding sites are functionally relevant and sufficient for EGR1-induced SOX18 transcription. Mutations of these binding sites significantly impaired activity of the SOX18 promoter, particularly in EA.hy926 cells, indicating the importance of these regulatory elements for SOX18 promoter activity in endothelial setting. By data presented in this study, we have established SOX18 as a novel target gene regulated by EGR1 transcription factor, thus providing the first functional link between two transcription factors previously shown to be involved in the control of angiogenesis.
PB  - Nature Publishing Group, New York
T2  - Experimental and Molecular Medicine
T1  - Early growth response protein 1 acts as an activator of SOX18 promoter
EP  - 142
IS  - 2
SP  - 132
VL  - 42
DO  - 10.3858/emm.2010.42.2.015
ER  - 

@article{
author = "Petrović, Isidora and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2010",
abstract = "Sex-determining region Y box 18 (Sox18/SOX18) gene is an important regulator of vascular development playing a role in endothelial cell specification or differentiation, angiogenesis and atherogenesis. The aim of this study was to perform comprehensive functional characterization of the human SOX18 promoter, including determination of transcription start point (tsp) and identification of control elements involved in the regulation of SOX18 gene expression, with an emphasis on angiogenesis-related transcription factors. Analyses were performed in HeLa cells, representing a tumor cell line, and in EA.hy926 cells used as an endothelial model system. We have determined unique tsp of SOX18 gene, located 172 nucleotides upstream from ATG codon. Further, we have shown that SOX18 promoter region, -726 to -89 by relative to tsp, contains positive cis-regulatory element(s) that stimulates SOX18 promoter activity, while region -89 to + 166 represents the minimal promoter. Within this region we have recognized the presence of essential element(s), positioned from -89 to +29, which harbors cluster of three putative early growth response 1 (EGR1) binding sites. By in vitro binding assays and functional analyses we have shown that these three putative binding sites are functionally relevant and sufficient for EGR1-induced SOX18 transcription. Mutations of these binding sites significantly impaired activity of the SOX18 promoter, particularly in EA.hy926 cells, indicating the importance of these regulatory elements for SOX18 promoter activity in endothelial setting. By data presented in this study, we have established SOX18 as a novel target gene regulated by EGR1 transcription factor, thus providing the first functional link between two transcription factors previously shown to be involved in the control of angiogenesis.",
publisher = "Nature Publishing Group, New York",
journal = "Experimental and Molecular Medicine",
title = "Early growth response protein 1 acts as an activator of SOX18 promoter",
pages = "142-132",
number = "2",
volume = "42",
doi = "10.3858/emm.2010.42.2.015"
}

Petrović, I., Kovačević Grujičić, N.,& Stevanović, M.. (2010). Early growth response protein 1 acts as an activator of SOX18 promoter. in Experimental and Molecular Medicine
Nature Publishing Group, New York., 42(2), 132-142.
https://doi.org/10.3858/emm.2010.42.2.015

Petrović I, Kovačević Grujičić N, Stevanović M. Early growth response protein 1 acts as an activator of SOX18 promoter. in Experimental and Molecular Medicine. 2010;42(2):132-142.
doi:10.3858/emm.2010.42.2.015 .

Petrović, Isidora, Kovačević Grujičić, Nataša, Stevanović, Milena, "Early growth response protein 1 acts as an activator of SOX18 promoter" in Experimental and Molecular Medicine, 42, no. 2 (2010):132-142,
https://doi.org/10.3858/emm.2010.42.2.015 . .

TY  - JOUR
AU  - Mojsin, Marija
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/404
AB  - Sox3/SOX3 [SRY (sex determining region Y)-box 3] is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. We have previously reported characterization of the SOX3 promoter and demonstrated that the general transcription factors NF-Y (nuclear factor-Y), Sp1 (specificity protein 1) and USF (upstream stimulatory factor) are involved in transcriptional regulation of SOX3 promoter activity. In the present study we provide the first evidence that the TALE (three-amino-acid loop extension) transcription factors PBX1 (pre-B-cell leukaemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue) participate in regulating human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus PBX/MEIS-binding site, which is conserved in all mammalian orthologue promoters analysed. PBX1 is present in the protein complex formed at this site with nuclear proteins from uninduced cells, whereas both PBX1 and MEIS1 proteins were detected in the complex created with extract from RA (retinoic acid)-induced NT2/D1 cells. By functional analysis we also showed that mutations of the PBX1/MEIS1-binding sites resulted in profound reduction of SOX3 promoter responsiveness to RA. Finally, we demonstrated that overexpressed PBX1 and MEIS1 increased endogenous SOX3 protein expression in both uninduced and RA-induced NT2/D1 cells. With the results of the present study, for the first time, we have established a functional link between the TALE proteins, PBX1 and MEIS1, and expression of the human SOX3 gene. This link is of particular interest since both TALE family members and members of the SOX superfamily are recognized as important developmental regulators.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region
EP  - 116
SP  - 107
VL  - 425
DO  - 10.1042/BJ20090694
ER  - 

@article{
author = "Mojsin, Marija and Stevanović, Milena",
year = "2010",
abstract = "Sox3/SOX3 [SRY (sex determining region Y)-box 3] is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. We have previously reported characterization of the SOX3 promoter and demonstrated that the general transcription factors NF-Y (nuclear factor-Y), Sp1 (specificity protein 1) and USF (upstream stimulatory factor) are involved in transcriptional regulation of SOX3 promoter activity. In the present study we provide the first evidence that the TALE (three-amino-acid loop extension) transcription factors PBX1 (pre-B-cell leukaemia homeobox 1) and MEIS1 (myeloid ecotropic viral integration site 1 homologue) participate in regulating human SOX3 gene expression in NT2/D1 cells by direct interaction with the consensus PBX/MEIS-binding site, which is conserved in all mammalian orthologue promoters analysed. PBX1 is present in the protein complex formed at this site with nuclear proteins from uninduced cells, whereas both PBX1 and MEIS1 proteins were detected in the complex created with extract from RA (retinoic acid)-induced NT2/D1 cells. By functional analysis we also showed that mutations of the PBX1/MEIS1-binding sites resulted in profound reduction of SOX3 promoter responsiveness to RA. Finally, we demonstrated that overexpressed PBX1 and MEIS1 increased endogenous SOX3 protein expression in both uninduced and RA-induced NT2/D1 cells. With the results of the present study, for the first time, we have established a functional link between the TALE proteins, PBX1 and MEIS1, and expression of the human SOX3 gene. This link is of particular interest since both TALE family members and members of the SOX superfamily are recognized as important developmental regulators.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region",
pages = "116-107",
volume = "425",
doi = "10.1042/BJ20090694"
}

Mojsin, M.,& Stevanović, M.. (2010). PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region. in Biochemical Journal
Portland Press Ltd, London., 425, 107-116.
https://doi.org/10.1042/BJ20090694

Mojsin M, Stevanović M. PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region. in Biochemical Journal. 2010;425:107-116.
doi:10.1042/BJ20090694 .

Mojsin, Marija, Stevanović, Milena, "PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region" in Biochemical Journal, 425 (2010):107-116,
https://doi.org/10.1042/BJ20090694 . .

TY  - JOUR
AU  - Milivojević, Milena
AU  - Nikčević, Gordana
AU  - Kovačević Grujičić, Nataša
AU  - Krstić, A.
AU  - Mojsin, Marija
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/448
AB  - SOX2 transkripcioni faktor ima ključnu ulogu u procesima embrionalnog razvića i predstavlja univerzalni marker pluripotentnih matičnih ćelija. S obzirom na funkcionalnu redundantnost i preklapajući profil ekspresije članova SOXB1 podgrupe tokom razvića, cilj ovog rada bio je da ispita potencijalne zajedničke aspekte regulacije ekspresije SOX2 i SOX3 gena. Naime, ispitivan je uticaj odabranih transkripcionih faktora na regulaciju ekspresije SOX2 gena u NT2/D1 ćelijskoj liniji. Analizirani su oni faktori za koje je prethodno pokazano da su uključeni u modulaciju aktivnosti humanog SOX3 gena. Rezultati ovih istraživanja ukazuju da opšti transkripcioni faktori (NF-Y, Sp1 i MAZ), članovi TALE familije proteina (Pbx1 i Meis1), kao i retinoičnom kiselinom aktiviran nuklearni receptor RXRα dovode do povećane ekspresije SOX2 proteina. Ispitivanje transkripcionih faktora uključenih u regulaciju ekspresije SOX gena je značajno za bolje razumevanje signalnih puteva koji su aktivni u pluripotentnim matičnim ćelijama.
AB  - SOX2 is a key transcription factor in embryonic development representing a universal marker of pluripotent stem cells. Based on the functional redundancy and overlapping expression patterns of SOXB1 subgroup members during development, the goal of this study has been to analyze if some aspects of regulation of expression are preserved between human SOX2 and SOX3 genes. Thus, we have tested several transcription factors previously demonstrated to play roles in controlling SOX3 gene activity for potential participation in the regulation of SOX2 gene expression in NT2/D1 cells. Here we report on the activation of SOX2 expression by ubiquitous transcription factors (NF-Y, Sp1 and MAZ), TALE family members (Pbx1 and Meis1), as well as liganded RXRα. Elucidating components involved in the regulation of SOX gene expression represent a valuable contribution in unraveling the regulatory networks operating in pluripotent embryonic cells.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji
T1  - Involvement of ubiquitous and tale transcription factors, as well as liganded RXRα, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line
EP  - 210
IS  - 2
SP  - 199
VL  - 62
DO  - 10.2298/ABS1002199M
ER  - 

@article{
author = "Milivojević, Milena and Nikčević, Gordana and Kovačević Grujičić, Nataša and Krstić, A. and Mojsin, Marija and Drakulić, Danijela and Stevanović, Milena",
year = "2010",
abstract = "SOX2 transkripcioni faktor ima ključnu ulogu u procesima embrionalnog razvića i predstavlja univerzalni marker pluripotentnih matičnih ćelija. S obzirom na funkcionalnu redundantnost i preklapajući profil ekspresije članova SOXB1 podgrupe tokom razvića, cilj ovog rada bio je da ispita potencijalne zajedničke aspekte regulacije ekspresije SOX2 i SOX3 gena. Naime, ispitivan je uticaj odabranih transkripcionih faktora na regulaciju ekspresije SOX2 gena u NT2/D1 ćelijskoj liniji. Analizirani su oni faktori za koje je prethodno pokazano da su uključeni u modulaciju aktivnosti humanog SOX3 gena. Rezultati ovih istraživanja ukazuju da opšti transkripcioni faktori (NF-Y, Sp1 i MAZ), članovi TALE familije proteina (Pbx1 i Meis1), kao i retinoičnom kiselinom aktiviran nuklearni receptor RXRα dovode do povećane ekspresije SOX2 proteina. Ispitivanje transkripcionih faktora uključenih u regulaciju ekspresije SOX gena je značajno za bolje razumevanje signalnih puteva koji su aktivni u pluripotentnim matičnim ćelijama., SOX2 is a key transcription factor in embryonic development representing a universal marker of pluripotent stem cells. Based on the functional redundancy and overlapping expression patterns of SOXB1 subgroup members during development, the goal of this study has been to analyze if some aspects of regulation of expression are preserved between human SOX2 and SOX3 genes. Thus, we have tested several transcription factors previously demonstrated to play roles in controlling SOX3 gene activity for potential participation in the regulation of SOX2 gene expression in NT2/D1 cells. Here we report on the activation of SOX2 expression by ubiquitous transcription factors (NF-Y, Sp1 and MAZ), TALE family members (Pbx1 and Meis1), as well as liganded RXRα. Elucidating components involved in the regulation of SOX gene expression represent a valuable contribution in unraveling the regulatory networks operating in pluripotent embryonic cells.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji, Involvement of ubiquitous and tale transcription factors, as well as liganded RXRα, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line",
pages = "210-199",
number = "2",
volume = "62",
doi = "10.2298/ABS1002199M"
}

Milivojević, M., Nikčević, G., Kovačević Grujičić, N., Krstić, A., Mojsin, M., Drakulić, D.,& Stevanović, M.. (2010). Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 199-210.
https://doi.org/10.2298/ABS1002199M

Milivojević M, Nikčević G, Kovačević Grujičić N, Krstić A, Mojsin M, Drakulić D, Stevanović M. Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji. in Archives of Biological Sciences. 2010;62(2):199-210.
doi:10.2298/ABS1002199M .

Milivojević, Milena, Nikčević, Gordana, Kovačević Grujičić, Nataša, Krstić, A., Mojsin, Marija, Drakulić, Danijela, Stevanović, Milena, "Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji" in Archives of Biological Sciences, 62, no. 2 (2010):199-210,
https://doi.org/10.2298/ABS1002199M . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Nikčević, Gordana
AU  - Zarić, Jelena
AU  - Ruegg, Curzio
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/431
AB  - Angiogenesis, the process of generating new blood vessels, is essential to embryonic development, organ formation, tissue regeneration and remodeling, reproduction and wound healing. Also, it plays an important role in many pathological conditions, including chronic inflammation and cancer. Angiogenesis is regulated by a complex interplay of growth factors, inflammatory mediators, adhesion molecules, morphogens and guidance molecules. Transcription factor SOX18 is transiently expressed in nascent endothelial cells during embryonic development and postnatal angiogenesis, but little is known about signaling pathways controlling its expression. The aim of this study was to investigate whether pro-angiogenic molecules and pharmacological inhibitors of angiogenesis modulate SOX18 expression in endothelial cells. Therefore, we treated human umbilical vein endothelial cells (HUVEC) with angiogenic factors, extracellular matrix proteins, inflammatory cytokines and nonsteroidal anti-inflammatory drugs (NSAID) and monitored SOX18 expression. We have observed that the angiogenic factor VEGF and the inflammatory cytokine TNF increase, while the NSAID ibuprofen and NS398 decrease the SOX18 protein level. These results for the first time demonstrate that SOX18 expression is modulated by factors and drugs known to positively or negatively regulate angiogenesis. This opens the possibility of pharmacological manipulation of SOX18 gene expression in endothelial cells to stimulate or inhibit angiogenesis.
PB  - Versita, Warsaw
T2  - Central European Journal of Biology
T1  - VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC
EP  - 434
IS  - 4
SP  - 427
VL  - 5
DO  - 10.2478/s11535-010-0031-3
ER  - 

@article{
author = "Petrović, Isidora and Nikčević, Gordana and Zarić, Jelena and Ruegg, Curzio and Stevanović, Milena",
year = "2010",
abstract = "Angiogenesis, the process of generating new blood vessels, is essential to embryonic development, organ formation, tissue regeneration and remodeling, reproduction and wound healing. Also, it plays an important role in many pathological conditions, including chronic inflammation and cancer. Angiogenesis is regulated by a complex interplay of growth factors, inflammatory mediators, adhesion molecules, morphogens and guidance molecules. Transcription factor SOX18 is transiently expressed in nascent endothelial cells during embryonic development and postnatal angiogenesis, but little is known about signaling pathways controlling its expression. The aim of this study was to investigate whether pro-angiogenic molecules and pharmacological inhibitors of angiogenesis modulate SOX18 expression in endothelial cells. Therefore, we treated human umbilical vein endothelial cells (HUVEC) with angiogenic factors, extracellular matrix proteins, inflammatory cytokines and nonsteroidal anti-inflammatory drugs (NSAID) and monitored SOX18 expression. We have observed that the angiogenic factor VEGF and the inflammatory cytokine TNF increase, while the NSAID ibuprofen and NS398 decrease the SOX18 protein level. These results for the first time demonstrate that SOX18 expression is modulated by factors and drugs known to positively or negatively regulate angiogenesis. This opens the possibility of pharmacological manipulation of SOX18 gene expression in endothelial cells to stimulate or inhibit angiogenesis.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Biology",
title = "VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC",
pages = "434-427",
number = "4",
volume = "5",
doi = "10.2478/s11535-010-0031-3"
}

Petrović, I., Nikčević, G., Zarić, J., Ruegg, C.,& Stevanović, M.. (2010). VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC. in Central European Journal of Biology
Versita, Warsaw., 5(4), 427-434.
https://doi.org/10.2478/s11535-010-0031-3

Petrović I, Nikčević G, Zarić J, Ruegg C, Stevanović M. VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC. in Central European Journal of Biology. 2010;5(4):427-434.
doi:10.2478/s11535-010-0031-3 .

Petrović, Isidora, Nikčević, Gordana, Zarić, Jelena, Ruegg, Curzio, Stevanović, Milena, "VEGF and TNF up-regulate, NSAID down-regulate SOX18 protein level in HUVEC" in Central European Journal of Biology, 5, no. 4 (2010):427-434,
https://doi.org/10.2478/s11535-010-0031-3 . .

TY  - JOUR
AU  - Stojiljković, Maja
AU  - Zukić, Branka
AU  - Tošić, Nataša
AU  - Karan-Đurašević, Teodora
AU  - Spasovski, Vesna
AU  - Nikčević, Gordana
AU  - Pavlović, Sonja
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/435
AB  - We present the first transcriptional regulatory element found in a PAH gene intron. The element is located in the PAH gene intron 8, acts as an enhancer specifically in the hepatoma cell line, and binds GATA-1 transcription factor. Herein the presented data could unlock a new area for the analysis of PAH gene expression and could contribute to refining genotype-phenotype correlation.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Molecular Genetics and Metabolism
T1  - Novel transcriptional regulatory element in the phenylalanine hydroxylase gene intron 8
EP  - 83
IS  - 1
SP  - 81
VL  - 101
DO  - 10.1016/j.ymgme.2010.05.014
ER  - 

@article{
author = "Stojiljković, Maja and Zukić, Branka and Tošić, Nataša and Karan-Đurašević, Teodora and Spasovski, Vesna and Nikčević, Gordana and Pavlović, Sonja",
year = "2010",
abstract = "We present the first transcriptional regulatory element found in a PAH gene intron. The element is located in the PAH gene intron 8, acts as an enhancer specifically in the hepatoma cell line, and binds GATA-1 transcription factor. Herein the presented data could unlock a new area for the analysis of PAH gene expression and could contribute to refining genotype-phenotype correlation.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Molecular Genetics and Metabolism",
title = "Novel transcriptional regulatory element in the phenylalanine hydroxylase gene intron 8",
pages = "83-81",
number = "1",
volume = "101",
doi = "10.1016/j.ymgme.2010.05.014"
}

Stojiljković, M., Zukić, B., Tošić, N., Karan-Đurašević, T., Spasovski, V., Nikčević, G.,& Pavlović, S.. (2010). Novel transcriptional regulatory element in the phenylalanine hydroxylase gene intron 8. in Molecular Genetics and Metabolism
Academic Press Inc Elsevier Science, San Diego., 101(1), 81-83.
https://doi.org/10.1016/j.ymgme.2010.05.014

Stojiljković M, Zukić B, Tošić N, Karan-Đurašević T, Spasovski V, Nikčević G, Pavlović S. Novel transcriptional regulatory element in the phenylalanine hydroxylase gene intron 8. in Molecular Genetics and Metabolism. 2010;101(1):81-83.
doi:10.1016/j.ymgme.2010.05.014 .

Stojiljković, Maja, Zukić, Branka, Tošić, Nataša, Karan-Đurašević, Teodora, Spasovski, Vesna, Nikčević, Gordana, Pavlović, Sonja, "Novel transcriptional regulatory element in the phenylalanine hydroxylase gene intron 8" in Molecular Genetics and Metabolism, 101, no. 1 (2010):81-83,
https://doi.org/10.1016/j.ymgme.2010.05.014 . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Lazić, Andrijana
AU  - Petrović, Isidora
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/430
AB  - The expression of Sox14 gene in spinal cord explants was found to be regulated by Sonic hedgehog (SHH) in a dose-dependent manner, indicating that this signaling molecule might act as a regulator of Sox14-expressing interneuron differentiation. In the present study we identified the positive control element and provided the first evidence that FOXA2 is involved in up-regulation of SOX14 expression in HepG2 and U87MG cell lines. By functional analysis we demonstrated that mutation in FOXA2 binding site reduced the SOX14 reporter construct activity, and that FOXA2 over-expression increased endogenous SOX14 protein expression. Further, we have shown that human SOX14 expression is GLI1 dependent in U87MG cells and SHH-N dependent in U87MG and HepG2 cell lines. By applying siRNA silencing of FOXA2, we have demonstrated that upregulation of endogenous SOX14 gene expression by SHH is, at least in part, mediated by FOXA2. However, our data revealed that a positive regulatory region, containing functional FOXA2 site analyzed in this study, is not involved in mediation of SHH dependent SOX14 activation. Data presented here provide the initial insight into molecular mechanism underlying tissue and developmentally specific regulation of the SOX14 gene expression.
PB  - Elsevier Science Bv, Amsterdam
T2  - Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms
T1  - Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression
EP  - 418
IS  - 5-6
SP  - 411
VL  - 1799
DO  - 10.1016/j.bbagrm.2010.01.002
ER  - 

@article{
author = "Popović, Jelena and Lazić, Andrijana and Petrović, Isidora and Stevanović, Milena",
year = "2010",
abstract = "The expression of Sox14 gene in spinal cord explants was found to be regulated by Sonic hedgehog (SHH) in a dose-dependent manner, indicating that this signaling molecule might act as a regulator of Sox14-expressing interneuron differentiation. In the present study we identified the positive control element and provided the first evidence that FOXA2 is involved in up-regulation of SOX14 expression in HepG2 and U87MG cell lines. By functional analysis we demonstrated that mutation in FOXA2 binding site reduced the SOX14 reporter construct activity, and that FOXA2 over-expression increased endogenous SOX14 protein expression. Further, we have shown that human SOX14 expression is GLI1 dependent in U87MG cells and SHH-N dependent in U87MG and HepG2 cell lines. By applying siRNA silencing of FOXA2, we have demonstrated that upregulation of endogenous SOX14 gene expression by SHH is, at least in part, mediated by FOXA2. However, our data revealed that a positive regulatory region, containing functional FOXA2 site analyzed in this study, is not involved in mediation of SHH dependent SOX14 activation. Data presented here provide the initial insight into molecular mechanism underlying tissue and developmentally specific regulation of the SOX14 gene expression.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms",
title = "Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression",
pages = "418-411",
number = "5-6",
volume = "1799",
doi = "10.1016/j.bbagrm.2010.01.002"
}

Popović, J., Lazić, A., Petrović, I.,& Stevanović, M.. (2010). Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression. in Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms
Elsevier Science Bv, Amsterdam., 1799(5-6), 411-418.
https://doi.org/10.1016/j.bbagrm.2010.01.002

Popović J, Lazić A, Petrović I, Stevanović M. Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression. in Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms. 2010;1799(5-6):411-418.
doi:10.1016/j.bbagrm.2010.01.002 .

Popović, Jelena, Lazić, Andrijana, Petrović, Isidora, Stevanović, Milena, "Tissue-specific Forkhead protein FOXA2 up-regulates SOX14 gene expression" in Biochimica Et Biophysica Acta-Gene Regulatory Mechanisms, 1799, no. 5-6 (2010):411-418,
https://doi.org/10.1016/j.bbagrm.2010.01.002 . .

TY  - JOUR
AU  - Savić, Tijana
AU  - Stevanović, Milena
AU  - Nikčević, Gordana
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/383
AB  - Sox3/SOX3 gen je uključen u kontrolu razvića nervnog sistema. Naša prethodna istraživanja su pokazala da tokom neuralne indukcije NT2/D1 ćelija retinoičnom kiselinom (RK) dolazi do promene ekspresije humanog SOX3 gena. Takođe, pokazali smo da su RXR retinoidni receptori glavni posrednici efekta RK na SOX3 ekspresiju, dok su članovi RAR familije receptora isključeni kao njihovi heterodimerni partneri u ovom signalnom putu. Rezultati predstavljeni u ovom radu ukazuju da je aktivacija SOX3 gena retinoičnom kiselinom nezavisna od RXR homodimera. Ova izučavanja ekspresije SOX3 gena su značajna za buduća istraživanja uticaja koji ovaj gen ima na različite aspekte normalnog i patološkog razvića.
AB  - The Sox3/SOX3 gene is implicated in the control of nervous system development. We previously demon­strated modulation of human SOX3 gene expression during neural induction of NT2/D1 cells by retinoic acid (RA). Also, we accurately verified RXR retinoid receptors as major mediators of the effect of RA on SOX3 expression, and excluded RARs as its heterodimeric partners in RA-SOX3 signaling. Here we present evidence that activation of the SOX3 gene by RA is not RXR homodimer-dependent. The described line of SOX3 gene expression studies is valuable for future investigation of the impact that this gene has multiple aspects of normal and pathological development.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Ekspresija SoX3 gena indukovana retinoičnom kiselinom u NT2/D1 ćelijama je nezavisna od RXr homodimera
T1  - Retinoic acid-induced SoX3 gene expression in NT2/D1 cells is RXR homodimer-independent
EP  - 638
IS  - 4
SP  - 631
VL  - 61
DO  - 10.2298/ABS0904631S
ER  - 

@article{
author = "Savić, Tijana and Stevanović, Milena and Nikčević, Gordana",
year = "2009",
abstract = "Sox3/SOX3 gen je uključen u kontrolu razvića nervnog sistema. Naša prethodna istraživanja su pokazala da tokom neuralne indukcije NT2/D1 ćelija retinoičnom kiselinom (RK) dolazi do promene ekspresije humanog SOX3 gena. Takođe, pokazali smo da su RXR retinoidni receptori glavni posrednici efekta RK na SOX3 ekspresiju, dok su članovi RAR familije receptora isključeni kao njihovi heterodimerni partneri u ovom signalnom putu. Rezultati predstavljeni u ovom radu ukazuju da je aktivacija SOX3 gena retinoičnom kiselinom nezavisna od RXR homodimera. Ova izučavanja ekspresije SOX3 gena su značajna za buduća istraživanja uticaja koji ovaj gen ima na različite aspekte normalnog i patološkog razvića., The Sox3/SOX3 gene is implicated in the control of nervous system development. We previously demon­strated modulation of human SOX3 gene expression during neural induction of NT2/D1 cells by retinoic acid (RA). Also, we accurately verified RXR retinoid receptors as major mediators of the effect of RA on SOX3 expression, and excluded RARs as its heterodimeric partners in RA-SOX3 signaling. Here we present evidence that activation of the SOX3 gene by RA is not RXR homodimer-dependent. The described line of SOX3 gene expression studies is valuable for future investigation of the impact that this gene has multiple aspects of normal and pathological development.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Ekspresija SoX3 gena indukovana retinoičnom kiselinom u NT2/D1 ćelijama je nezavisna od RXr homodimera, Retinoic acid-induced SoX3 gene expression in NT2/D1 cells is RXR homodimer-independent",
pages = "638-631",
number = "4",
volume = "61",
doi = "10.2298/ABS0904631S"
}

Savić, T., Stevanović, M.,& Nikčević, G.. (2009). Ekspresija SoX3 gena indukovana retinoičnom kiselinom u NT2/D1 ćelijama je nezavisna od RXr homodimera. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 61(4), 631-638.
https://doi.org/10.2298/ABS0904631S

Savić T, Stevanović M, Nikčević G. Ekspresija SoX3 gena indukovana retinoičnom kiselinom u NT2/D1 ćelijama je nezavisna od RXr homodimera. in Archives of Biological Sciences. 2009;61(4):631-638.
doi:10.2298/ABS0904631S .

Savić, Tijana, Stevanović, Milena, Nikčević, Gordana, "Ekspresija SoX3 gena indukovana retinoičnom kiselinom u NT2/D1 ćelijama je nezavisna od RXr homodimera" in Archives of Biological Sciences, 61, no. 4 (2009):631-638,
https://doi.org/10.2298/ABS0904631S . .

TY  - JOUR
AU  - Petrović, Isidora
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/359
AB  - The aim of this study has been to identify transcription factors involved in transcriptional regulation of the human SOX18 gene expression. Structural analysis revealed that the SOX18 promoter lacks a TATA box, but is CG-rich containing many putative binding sites for transcription factors that can bind and act through GC-boxes. Alignment analysis of promoter regions between human and mouse revealed conserved putative binding sites for transcription factors NF-Y and Sp-family members. Mithramycin A treatment led to increased SOX18 expression in vivo raising the possibility that the GC-rich sequence of the human SOX18 promoter might be occupied by transcription factor(s) that acts as repressor(s). Using in vitro binding assays we have demonstrated that transcription factors Sp3, ZBP-89 and NF-Y are capable of binding to the SOX18 promoter region spanning the sequence -200 to -162 relative to ATG and that formation of complexes could be efficiently reduced by mithramycin A. Furthermore, co-transfection experiments revealed that over-expression of Sp3 and ZBP-89 down-regulate, while over-expression of NF-Y up-regulates SOX18 promoter activity in HeLa cells. The involvement of these transcription factors in the regulation of SOX18 expression in HeLa cells was further confirmed in vivo by Western blot analyses. In this paper, for the first time, we have demonstrated that Sp3, ZBP-89 and NF-Y are involved in transcriptional regulation of the human SOX18 gene expression. Presented data provide the initial information about transcriptional regulation that will help in better understanding of molecular mechanisms involved in regulation of SOX18 gene expression.
PB  - Springer, Dordrecht
T2  - Molecular Biology Reports
T1  - ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells
EP  - 1000
IS  - 5
SP  - 993
VL  - 36
DO  - 10.1007/s11033-008-9272-x
ER  - 

@article{
author = "Petrović, Isidora and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2009",
abstract = "The aim of this study has been to identify transcription factors involved in transcriptional regulation of the human SOX18 gene expression. Structural analysis revealed that the SOX18 promoter lacks a TATA box, but is CG-rich containing many putative binding sites for transcription factors that can bind and act through GC-boxes. Alignment analysis of promoter regions between human and mouse revealed conserved putative binding sites for transcription factors NF-Y and Sp-family members. Mithramycin A treatment led to increased SOX18 expression in vivo raising the possibility that the GC-rich sequence of the human SOX18 promoter might be occupied by transcription factor(s) that acts as repressor(s). Using in vitro binding assays we have demonstrated that transcription factors Sp3, ZBP-89 and NF-Y are capable of binding to the SOX18 promoter region spanning the sequence -200 to -162 relative to ATG and that formation of complexes could be efficiently reduced by mithramycin A. Furthermore, co-transfection experiments revealed that over-expression of Sp3 and ZBP-89 down-regulate, while over-expression of NF-Y up-regulates SOX18 promoter activity in HeLa cells. The involvement of these transcription factors in the regulation of SOX18 expression in HeLa cells was further confirmed in vivo by Western blot analyses. In this paper, for the first time, we have demonstrated that Sp3, ZBP-89 and NF-Y are involved in transcriptional regulation of the human SOX18 gene expression. Presented data provide the initial information about transcriptional regulation that will help in better understanding of molecular mechanisms involved in regulation of SOX18 gene expression.",
publisher = "Springer, Dordrecht",
journal = "Molecular Biology Reports",
title = "ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells",
pages = "1000-993",
number = "5",
volume = "36",
doi = "10.1007/s11033-008-9272-x"
}

Petrović, I., Kovačević Grujičić, N.,& Stevanović, M.. (2009). ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. in Molecular Biology Reports
Springer, Dordrecht., 36(5), 993-1000.
https://doi.org/10.1007/s11033-008-9272-x

Petrović I, Kovačević Grujičić N, Stevanović M. ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells. in Molecular Biology Reports. 2009;36(5):993-1000.
doi:10.1007/s11033-008-9272-x .

Petrović, Isidora, Kovačević Grujičić, Nataša, Stevanović, Milena, "ZBP-89 and Sp3 down-regulate while NF-Y up-regulates SOX18 promoter activity in HeLa cells" in Molecular Biology Reports, 36, no. 5 (2009):993-1000,
https://doi.org/10.1007/s11033-008-9272-x . .

TY  - JOUR
AU  - Popović, Jelena
AU  - Stevanović, Milena
PY  - 2009
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/368
AB  - SOX proteins constitute a large family of diverse, well-conserved transcription factors present in vertebrates and invertebrates, and also implicated in control of many developmental processes. Our objectives have been to identify Sox14 gene of goat (Capra hircus), cow (Bos taurus) and rat (Rattus norvegicus), and to perform comparative analyses and mapping of SOX14 orthologues from numerous vertebrate species. PCR based approach was used to identify Sox14 of goat, cow and rat, while nucleotide and amino acid sequence alignments and mapping were performed using information currently available in public database. Comparative sequence analysis revealed remarkable identity among Sox14 orthologues and helped us to identify highly conserved motifs that represent molecular signatures of SOX14 protein that might have structural or functional significance. Further, we determined chromosomal locations of numerous predicted group B Sox genes and their neighbouring genes using currently available genome database. In conclusion, our study has not only supported the proposed model of group B Sox genes evolution in chicken and mammals, but has also revealed that additional evolutionary events split Sox B genes into different chromosomes in some mammals. Mapping data presented in this study could help in refining the understanding of the evolution of group B Sox genes in vertebrates.
PB  - Indian Acad Sciences, Bangalore
T2  - Journal of Genetics
T1  - Remarkable evolutionary conservation of SOX14 orthologues
EP  - 24
IS  - 1
SP  - 15
VL  - 88
DO  - 10.1007/s12041-009-0003-4
ER  - 

@article{
author = "Popović, Jelena and Stevanović, Milena",
year = "2009",
abstract = "SOX proteins constitute a large family of diverse, well-conserved transcription factors present in vertebrates and invertebrates, and also implicated in control of many developmental processes. Our objectives have been to identify Sox14 gene of goat (Capra hircus), cow (Bos taurus) and rat (Rattus norvegicus), and to perform comparative analyses and mapping of SOX14 orthologues from numerous vertebrate species. PCR based approach was used to identify Sox14 of goat, cow and rat, while nucleotide and amino acid sequence alignments and mapping were performed using information currently available in public database. Comparative sequence analysis revealed remarkable identity among Sox14 orthologues and helped us to identify highly conserved motifs that represent molecular signatures of SOX14 protein that might have structural or functional significance. Further, we determined chromosomal locations of numerous predicted group B Sox genes and their neighbouring genes using currently available genome database. In conclusion, our study has not only supported the proposed model of group B Sox genes evolution in chicken and mammals, but has also revealed that additional evolutionary events split Sox B genes into different chromosomes in some mammals. Mapping data presented in this study could help in refining the understanding of the evolution of group B Sox genes in vertebrates.",
publisher = "Indian Acad Sciences, Bangalore",
journal = "Journal of Genetics",
title = "Remarkable evolutionary conservation of SOX14 orthologues",
pages = "24-15",
number = "1",
volume = "88",
doi = "10.1007/s12041-009-0003-4"
}

Popović, J.,& Stevanović, M.. (2009). Remarkable evolutionary conservation of SOX14 orthologues. in Journal of Genetics
Indian Acad Sciences, Bangalore., 88(1), 15-24.
https://doi.org/10.1007/s12041-009-0003-4

Popović J, Stevanović M. Remarkable evolutionary conservation of SOX14 orthologues. in Journal of Genetics. 2009;88(1):15-24.
doi:10.1007/s12041-009-0003-4 .

Popović, Jelena, Stevanović, Milena, "Remarkable evolutionary conservation of SOX14 orthologues" in Journal of Genetics, 88, no. 1 (2009):15-24,
https://doi.org/10.1007/s12041-009-0003-4 . .

TY  - JOUR
AU  - Krstić, A.
AU  - Mojsin, Marija
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/332
AB  - Sox3 gen je jedan od markera najranijih faza razvića nervnog sistema kičmenjaka koji je uključen u kontrolu diferencijacije nervnih prekursora. Uprkos činjenici da je genom pacova sekvenciran i javno dostupan, samo parcijalna sekvenca Sox3 gena ove vrste je bila deponovana u bazi podataka. U ovom radu smo primenom PCR-a, sekvenciranja i bioinformatičke analize generisali kompletnu kodirajuću sekvencu Sox3 gena pacova. Analiza dobijene sekvence je pokazala da Sox3 gen kodira protein od 449 amino kiselina. Uporedna analiza ortologih SOX3 proteina pacova i čoveka pokazala je visok stepen evolutivne očuvanosti. Identifikacija i karakterizacija Sox3 gena pacova doprineće boljem razumevanju njegove uloge tokom razvića nervnog sistema i omogućiće bolji uvid u evoluciju ovog gena kod vertebrata.
AB  - The Sox3 gene is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Despite the completion of a rat genome sequencing project, only a partial sequence of the rat Sox3 gene has been available in the public database. Using PCR, sequencing, and bioinformatics tools, in this study we have determined the complete coding sequence of the rat Sox3 gene encoding 449 amino acids. Comparative analysis of rat and human SOX3 proteins revealed a high degree of conservation. Identification of the rat Sox3 gene sequence would help in understanding the biological roles of this gene and provide insight into evolutionary relationships with vertebrate orthologs.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Amplifikacija i analiza sekvence Sox3 gena pacova
T1  - PCR amplification and sequence analysis of the rat Sox3 gene
EP  - 530
IS  - 4
SP  - 525
VL  - 60
DO  - 10.2298/ABS0804525K
ER  - 

@article{
author = "Krstić, A. and Mojsin, Marija and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2008",
abstract = "Sox3 gen je jedan od markera najranijih faza razvića nervnog sistema kičmenjaka koji je uključen u kontrolu diferencijacije nervnih prekursora. Uprkos činjenici da je genom pacova sekvenciran i javno dostupan, samo parcijalna sekvenca Sox3 gena ove vrste je bila deponovana u bazi podataka. U ovom radu smo primenom PCR-a, sekvenciranja i bioinformatičke analize generisali kompletnu kodirajuću sekvencu Sox3 gena pacova. Analiza dobijene sekvence je pokazala da Sox3 gen kodira protein od 449 amino kiselina. Uporedna analiza ortologih SOX3 proteina pacova i čoveka pokazala je visok stepen evolutivne očuvanosti. Identifikacija i karakterizacija Sox3 gena pacova doprineće boljem razumevanju njegove uloge tokom razvića nervnog sistema i omogućiće bolji uvid u evoluciju ovog gena kod vertebrata., The Sox3 gene is considered to be one of the earliest neural markers in vertebrates, playing a role in specifying neuronal fate. Despite the completion of a rat genome sequencing project, only a partial sequence of the rat Sox3 gene has been available in the public database. Using PCR, sequencing, and bioinformatics tools, in this study we have determined the complete coding sequence of the rat Sox3 gene encoding 449 amino acids. Comparative analysis of rat and human SOX3 proteins revealed a high degree of conservation. Identification of the rat Sox3 gene sequence would help in understanding the biological roles of this gene and provide insight into evolutionary relationships with vertebrate orthologs.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Amplifikacija i analiza sekvence Sox3 gena pacova, PCR amplification and sequence analysis of the rat Sox3 gene",
pages = "530-525",
number = "4",
volume = "60",
doi = "10.2298/ABS0804525K"
}

Krstić, A., Mojsin, M., Kovačević Grujičić, N.,& Stevanović, M.. (2008). Amplifikacija i analiza sekvence Sox3 gena pacova. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(4), 525-530.
https://doi.org/10.2298/ABS0804525K

Krstić A, Mojsin M, Kovačević Grujičić N, Stevanović M. Amplifikacija i analiza sekvence Sox3 gena pacova. in Archives of Biological Sciences. 2008;60(4):525-530.
doi:10.2298/ABS0804525K .

Krstić, A., Mojsin, Marija, Kovačević Grujičić, Nataša, Stevanović, Milena, "Amplifikacija i analiza sekvence Sox3 gena pacova" in Archives of Biological Sciences, 60, no. 4 (2008):525-530,
https://doi.org/10.2298/ABS0804525K . .

TY  - JOUR
AU  - Nikčević, Gordana
AU  - Savić, Tijana
AU  - Kovačević Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/301
AB  - Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates and it is implicated in the genetic cascades that direct brain formation. We have previously shown that early phases of differentiation and neural induction of NT2/D1 embryonal carcinoma cells by retinoic acid ( RA) involve up-regulation of the SOX3 gene expression. Here, we present identification of a novel positive regulatory promoter element involved in RA-dependent activation of the SOX3 gene expression in NT2/D1 cells. This element represents a direct repeat 3-like motif that directly interacts with retinoid X receptor (RXR) alpha in a sequence-specific manner. It is capable of independently mediating the RA effect in a heterologous promoter context and its disruption caused significant reduction of RA/RXR transactivation of the SOX3 promoter. Furthermore, by using synthetic antagonists of retinoid receptors, we have shown for the first time, that RA-induced SOX3 gene expression could be significantly down-regulated by the synthetic antagonist of RXR. Also, this data showed that RXRs, but not RA receptors, are mediators of RA effect on the SOX3 gene up-regulation in NT2/D1 cells. Presented data will be valuable for future investigation of SOX3 gene expression, not only in NT2/D1 model system, but also in diverse developmental, physiological and pathological settings.
PB  - Wiley, Hoboken
T2  - Journal of Neurochemistry
T1  - Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved
EP  - 1215
IS  - 5
SP  - 1206
VL  - 107
DO  - 10.1111/j.1471-4159.2008.05670.x
ER  - 

@article{
author = "Nikčević, Gordana and Savić, Tijana and Kovačević Grujičić, Nataša and Stevanović, Milena",
year = "2008",
abstract = "Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates and it is implicated in the genetic cascades that direct brain formation. We have previously shown that early phases of differentiation and neural induction of NT2/D1 embryonal carcinoma cells by retinoic acid ( RA) involve up-regulation of the SOX3 gene expression. Here, we present identification of a novel positive regulatory promoter element involved in RA-dependent activation of the SOX3 gene expression in NT2/D1 cells. This element represents a direct repeat 3-like motif that directly interacts with retinoid X receptor (RXR) alpha in a sequence-specific manner. It is capable of independently mediating the RA effect in a heterologous promoter context and its disruption caused significant reduction of RA/RXR transactivation of the SOX3 promoter. Furthermore, by using synthetic antagonists of retinoid receptors, we have shown for the first time, that RA-induced SOX3 gene expression could be significantly down-regulated by the synthetic antagonist of RXR. Also, this data showed that RXRs, but not RA receptors, are mediators of RA effect on the SOX3 gene up-regulation in NT2/D1 cells. Presented data will be valuable for future investigation of SOX3 gene expression, not only in NT2/D1 model system, but also in diverse developmental, physiological and pathological settings.",
publisher = "Wiley, Hoboken",
journal = "Journal of Neurochemistry",
title = "Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved",
pages = "1215-1206",
number = "5",
volume = "107",
doi = "10.1111/j.1471-4159.2008.05670.x"
}

Nikčević, G., Savić, T., Kovačević Grujičić, N.,& Stevanović, M.. (2008). Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved. in Journal of Neurochemistry
Wiley, Hoboken., 107(5), 1206-1215.
https://doi.org/10.1111/j.1471-4159.2008.05670.x

Nikčević G, Savić T, Kovačević Grujičić N, Stevanović M. Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved. in Journal of Neurochemistry. 2008;107(5):1206-1215.
doi:10.1111/j.1471-4159.2008.05670.x .

Nikčević, Gordana, Savić, Tijana, Kovačević Grujičić, Nataša, Stevanović, Milena, "Up-regulation of the SOX3 gene expression by retinoic acid: characterization of the novel promoter-response element and the retinoid receptors involved" in Journal of Neurochemistry, 107, no. 5 (2008):1206-1215,
https://doi.org/10.1111/j.1471-4159.2008.05670.x . .

TY  - JOUR
AU  - Cuturilo, Goran
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
AU  - Jovanović, Ida
AU  - Đukić, Milan
AU  - Miletić-Grković, Slobodanka
AU  - Atanasković-Marković, Marina
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/297
AB  - Microdeletion 22q11.2 is associated with a variety of findings, and the most common are cardiac defects. It is very frequently associated with interrupted aortic arch (IAA) type B and very rarely with type A and type C. Here we report the first case of IAA type C associated with 22q11.2 deletion in Serbia and, to the best of our knowledge, the fourth case described worldwide so far. By this report we would like to point out that all patients with IAA type C who have additional features specific for 22q11.2 microdeletion syndrome should be screened for the presence of this deletion.
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - A rare association of interrupted aortic arch type C and microdeletion 22q11.2
EP  - 1198
IS  - 10
SP  - 1195
VL  - 167
DO  - 10.1007/s00431-007-0632-7
ER  - 

@article{
author = "Cuturilo, Goran and Drakulić, Danijela and Stevanović, Milena and Jovanović, Ida and Đukić, Milan and Miletić-Grković, Slobodanka and Atanasković-Marković, Marina",
year = "2008",
abstract = "Microdeletion 22q11.2 is associated with a variety of findings, and the most common are cardiac defects. It is very frequently associated with interrupted aortic arch (IAA) type B and very rarely with type A and type C. Here we report the first case of IAA type C associated with 22q11.2 deletion in Serbia and, to the best of our knowledge, the fourth case described worldwide so far. By this report we would like to point out that all patients with IAA type C who have additional features specific for 22q11.2 microdeletion syndrome should be screened for the presence of this deletion.",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "A rare association of interrupted aortic arch type C and microdeletion 22q11.2",
pages = "1198-1195",
number = "10",
volume = "167",
doi = "10.1007/s00431-007-0632-7"
}

Cuturilo, G., Drakulić, D., Stevanović, M., Jovanović, I., Đukić, M., Miletić-Grković, S.,& Atanasković-Marković, M.. (2008). A rare association of interrupted aortic arch type C and microdeletion 22q11.2. in European Journal of Pediatrics
Springer, New York., 167(10), 1195-1198.
https://doi.org/10.1007/s00431-007-0632-7

Cuturilo G, Drakulić D, Stevanović M, Jovanović I, Đukić M, Miletić-Grković S, Atanasković-Marković M. A rare association of interrupted aortic arch type C and microdeletion 22q11.2. in European Journal of Pediatrics. 2008;167(10):1195-1198.
doi:10.1007/s00431-007-0632-7 .

Cuturilo, Goran, Drakulić, Danijela, Stevanović, Milena, Jovanović, Ida, Đukić, Milan, Miletić-Grković, Slobodanka, Atanasković-Marković, Marina, "A rare association of interrupted aortic arch type C and microdeletion 22q11.2" in European Journal of Pediatrics, 167, no. 10 (2008):1195-1198,
https://doi.org/10.1007/s00431-007-0632-7 . .

TY  - JOUR
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Đurović, Jelena
AU  - Petrović, Isidora
AU  - Stevanović, Milena
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/299
AB  - SOX proteins constitute a large family of diverse and well conserved transcription factors implicated in the control of various developmental processes. Previously we have cloned and characterized human SOX3, SOX14 and SOX18 genes and performed functional characterization of their promoter regions. To better understand organization and function of SOX3, SOX14 and SOX18 promoters and to determine evolutionary conserved regulatory regions, we performed comparative genomic analyses of orthologous genes promoters. Mammalian orthologs of the human SOX3, SOX14 and SOX18 genes show high sequence identity in their promoter regions, particularly within basal promoters of the respective human genes. Binding sites for transcription factors NF-Y, Sp1 and USF1, previously shown to play critical roles in transcriptional regulation of these human genes, are highly conserved in sequence and position among diverse mammalian species. Conservation of binding sites might indicate their highly significant roles in maintaining the transcriptional regulation of these genes among different species.
PB  - Informa Healthcare, New York
T2  - DNA Sequence
T1  - Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals
EP  - 194
IS  - 3
SP  - 185
VL  - 19
DO  - 10.1080/10425170701462092
ER  - 

@article{
author = "Kovačević Grujičić, Nataša and Mojsin, Marija and Đurović, Jelena and Petrović, Isidora and Stevanović, Milena",
year = "2008",
abstract = "SOX proteins constitute a large family of diverse and well conserved transcription factors implicated in the control of various developmental processes. Previously we have cloned and characterized human SOX3, SOX14 and SOX18 genes and performed functional characterization of their promoter regions. To better understand organization and function of SOX3, SOX14 and SOX18 promoters and to determine evolutionary conserved regulatory regions, we performed comparative genomic analyses of orthologous genes promoters. Mammalian orthologs of the human SOX3, SOX14 and SOX18 genes show high sequence identity in their promoter regions, particularly within basal promoters of the respective human genes. Binding sites for transcription factors NF-Y, Sp1 and USF1, previously shown to play critical roles in transcriptional regulation of these human genes, are highly conserved in sequence and position among diverse mammalian species. Conservation of binding sites might indicate their highly significant roles in maintaining the transcriptional regulation of these genes among different species.",
publisher = "Informa Healthcare, New York",
journal = "DNA Sequence",
title = "Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals",
pages = "194-185",
number = "3",
volume = "19",
doi = "10.1080/10425170701462092"
}

Kovačević Grujičić, N., Mojsin, M., Đurović, J., Petrović, I.,& Stevanović, M.. (2008). Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals. in DNA Sequence
Informa Healthcare, New York., 19(3), 185-194.
https://doi.org/10.1080/10425170701462092

Kovačević Grujičić N, Mojsin M, Đurović J, Petrović I, Stevanović M. Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals. in DNA Sequence. 2008;19(3):185-194.
doi:10.1080/10425170701462092 .

Kovačević Grujičić, Nataša, Mojsin, Marija, Đurović, Jelena, Petrović, Isidora, Stevanović, Milena, "Comparison of promoter regions of SOX3, SOX14 and SOX18 orthologs in mammals" in DNA Sequence, 19, no. 3 (2008):185-194,
https://doi.org/10.1080/10425170701462092 . .

TY  - JOUR
AU  - Kovačević Grujičić, Nataša
AU  - Yokoyama, Kazunari K.
AU  - Stevanović, Milena
PY  - 2008
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/334
AB  - U ovom radu proučavana je uloga tri visoko konzervisana potencijalna mesta vezivanja za "Myc-associated zinc finger protein" (MAZ) u regulaciji ekspresije humanog SOX3 gena. Eseji izmenjene elektroforetske pokretljivosti u prisustvu antitela na MAZ ukazuju da kompleksi koji se formiraju na dva od tri proučavana mesta u okviru SOX3 promotora sadrže MAZ protein. Takođe, u eksperimentima kotransfekcije smo pokazali da MAZ ima ulogu pozitivnog regulatora transkripcije SOX3 gena, kako u nediferenciranim, tako i u diferenciranim NT2/D1 ćelijama. Iako je MAZ povećao i bazalnu i retinoičnom kiselinom indukovanu promotorsku aktivnost, naši rezultati ukazuju da ovaj transkripcioni faktor ne doprinosi inducibilnosti SOX3 promotora tokom neuralne diferencijacije u prisustvu retinoične kiseline.
AB  - In this study, we examine the role of three highly conserved putative binding sites for Myc-associated zinc finger protein (MAZ) in regulation of the human SOX3 gene expression. Electrophoretic mobility shift and supershift assays indicate that complexes formed at two out of three MAZ sites of the human SOX3 promoter involve ubiquitously expressed MAZ protein. Furthermore, in cotransfection experiments we demonstrate that MAZ acts as a positive regulator of SOX3 gene transcription in both undifferentiated and RA-differentiated NT2/D1 cells. Although MAZ increased both basal and RA-induced promoter activity, our results suggest that MAZ does not contribute to RA inducibility of the SOX3 promoter during neuronal differentiation of NT2/D1 cells.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Trans-aktivacija humanog SOX3 promotora MAZ proteinom u NT2/D1 ćelijama
T1  - Trans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells
EP  - 387
IS  - 3
SP  - 379
VL  - 60
DO  - 10.2298/ABS0803379K
ER  - 

@article{
author = "Kovačević Grujičić, Nataša and Yokoyama, Kazunari K. and Stevanović, Milena",
year = "2008",
abstract = "U ovom radu proučavana je uloga tri visoko konzervisana potencijalna mesta vezivanja za "Myc-associated zinc finger protein" (MAZ) u regulaciji ekspresije humanog SOX3 gena. Eseji izmenjene elektroforetske pokretljivosti u prisustvu antitela na MAZ ukazuju da kompleksi koji se formiraju na dva od tri proučavana mesta u okviru SOX3 promotora sadrže MAZ protein. Takođe, u eksperimentima kotransfekcije smo pokazali da MAZ ima ulogu pozitivnog regulatora transkripcije SOX3 gena, kako u nediferenciranim, tako i u diferenciranim NT2/D1 ćelijama. Iako je MAZ povećao i bazalnu i retinoičnom kiselinom indukovanu promotorsku aktivnost, naši rezultati ukazuju da ovaj transkripcioni faktor ne doprinosi inducibilnosti SOX3 promotora tokom neuralne diferencijacije u prisustvu retinoične kiseline., In this study, we examine the role of three highly conserved putative binding sites for Myc-associated zinc finger protein (MAZ) in regulation of the human SOX3 gene expression. Electrophoretic mobility shift and supershift assays indicate that complexes formed at two out of three MAZ sites of the human SOX3 promoter involve ubiquitously expressed MAZ protein. Furthermore, in cotransfection experiments we demonstrate that MAZ acts as a positive regulator of SOX3 gene transcription in both undifferentiated and RA-differentiated NT2/D1 cells. Although MAZ increased both basal and RA-induced promoter activity, our results suggest that MAZ does not contribute to RA inducibility of the SOX3 promoter during neuronal differentiation of NT2/D1 cells.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Trans-aktivacija humanog SOX3 promotora MAZ proteinom u NT2/D1 ćelijama, Trans-activation of the human SOX3 promoter by MAZ in NT2/D1 cells",
pages = "387-379",
number = "3",
volume = "60",
doi = "10.2298/ABS0803379K"
}

Kovačević Grujičić, N., Yokoyama, K. K.,& Stevanović, M.. (2008). Trans-aktivacija humanog SOX3 promotora MAZ proteinom u NT2/D1 ćelijama. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(3), 379-387.
https://doi.org/10.2298/ABS0803379K

Kovačević Grujičić N, Yokoyama KK, Stevanović M. Trans-aktivacija humanog SOX3 promotora MAZ proteinom u NT2/D1 ćelijama. in Archives of Biological Sciences. 2008;60(3):379-387.
doi:10.2298/ABS0803379K .

Kovačević Grujičić, Nataša, Yokoyama, Kazunari K., Stevanović, Milena, "Trans-aktivacija humanog SOX3 promotora MAZ proteinom u NT2/D1 ćelijama" in Archives of Biological Sciences, 60, no. 3 (2008):379-387,
https://doi.org/10.2298/ABS0803379K . .