Pretraživanje
Prikaz rezultata 31-40 od 59
Efficient transformation of Micromonospora melanosporea protoplasts by Streptomyces plasmid
(Springer-Verlag, 1991)
An efficient and relatively simple procedure for Micromonospora melanosporea protoplast preparation and transformation is described. Transformation of M. melanosporea protoplast by the Streptomyces plasmid pIJ702 was ...
In vivo and in vitro activities of the Escherichia coli sigma(54) transcription activator, PspF, and its DNA-binding mutant, PspF Delta HTH
(Academic Press Ltd- Elsevier Science Ltd, London, 1999)
Transcription of the phage-shock protein (psp) operon in Escherichia coli is driven by a sigma(54) promoter, stimulated by integration host factor and dependent on an upstream, cis-acting sequence and an activator protein, ...
Cloning and characterization of an aminoglycoside resistance determinant from Micromonospora zionensis
(Journal of Bacteriology, 1992)
The sisomicin-gentamicin resistance methylase (sgm) gene was isolated from Micromonospora zionensis and cloned in Streptomyces lividans. The sgm gene was expressed in Micromonospora melanosporea, where its own promoter was ...
Characterization of natural isolate Lactobacillus acidophilus BGRA43 useful for acidophilus milk production
(Wiley, Hoboken, 1998)
Lactobacillus acidophilus BGRA43 was selected from a set of human origin isolates of Lact. acidophilus strains for the highest growth rates and antagonistic effect against both Gram-positive and Gram-negative bacteria. The ...
Campomelic dysplasia and autosomal sex reversal caused by mutations in an SRY-related gene
(Nature, 1994)
Induction of testis development in mammals requires the presence of the Y-chromosome gene SPY. This gene must exert its effect by interacting with other genes in the sex-determination pathway. Cloning of a translocation ...
Analiza regulatornih regiona i ekspresija sgm gena bakterije Micromonospora zionensis / Analysis of regulatory regions and expression of the sgm gene from Micromonospora zionensis
(Univerzitet u Beogradu, Biološki fakultet, 1994)
Proizvodjac aminoglikozidnog antibiotika G-52, Micromonospora zionensis, poseduje sgm gen koji kodira 16$ rRNK metilazu cijom se ak'uvnoscu ostvaruje rezistencija na 4,6-disupstituisane dezoksistreptaminske aminoglikozide. ...
Role of upstream activation sequences and integration host factor in transcriptional activation by the constitutively active prokaryotic enhancer-binding protein PspF
(Academic Press Ltd, London, 1997)
PspF, the transcriptional activator of the pspA operon of Escherichia coli, which belongs to the enhancer binding protein (EBP) family of sigma(54) activator proteins, is constitutively active in an in vitro transcription ...
DNA sequence analysis of spontaneous histidine mutations in a polA1 strain of Escherichia coli K12 suggests a specific role of the GTGG sequence
(Springer-Verlag, 1990)
Spontaneously arising histidine mutations in an Escherichia coli K12 strain deficient for DNA polymerase I were analysed at the DNA sequence level. We screened approximately 150000 colonies and isolated 106 histidine ...
Hybrid P(L)tl promoter with dual regulation control
(DNA and Cell Biology, 1991)
The newly constructed P(L)tl hybrid promoter is composed of the operator and promoter sequences of tac and the -35 to -135 region of the phage λ P(L) promoter, which contains the AT-rich block and the OL2 and OL3 segments. ...
Analiza velikog konjugabilnog plazmida bakterije Lactococcus lactis subsp. lactis biovar. diacetylactis S50 / Analysis of large conjugative plasmid of Lactococcus lactis subsp. lactis biovar. diacetylactis S50
(Univerzitet u Beogradu, Biološki fakultet, 1995)
Lactococcus lactis subsp. lactis biovar. diacetylactis S50 je prirodni izolat iz maslacne maje. Soj S50 sintetise bakteriocin uskog spektra delovanja i proteinazu PI tipa. Soj S50 poseduje tri vidijiva plazmida koji se ...