TY  - JOUR
AU  - Soković Bajić, Svetlana
AU  - Mihajlović, Sanja B.
AU  - Radojević, Dušan
AU  - Popović, Dušanka
AU  - Đokić, Jelena
AU  - Stanisavljević, Suzana
AU  - Lazarević, Milica N.
AU  - Miljković, Djordje M.
AU  - Ruas-Madiedo, Patricia
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1386
AB  - Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. The aim of this work was to study the probiotic effect of gamma-aminobutyric acid (GABA)-producer Lactobacillus brevis BGZLS10-17 on experimental autoimmune encephalomyelitis (EAE), an experimental animal MS model. Clinical EAE symptoms were monitored in Dark Agouti (DA) rats treated with L. brevis BGZLS10-17 strain, or supernatant obtained from 48 h culture of L. brevis BGZLS10-17 cultivated in growth medium with or without GABA precursor monosodium glutamate (MSG). The results revealed that oral administration of L. brevis BGZLS10-17 alleviates the symptoms of EAE in DA rats. Namely, treatment with BGZLS10-17 and the supernatant of the strain cultivated in medium with MSG delayed the onset, shortened the duration, and reduced the intensity of the disease in the period when the EAE symptoms in controls were most pronounced. The probiotic treated animals were completely recovered after forty days, unlike the control animals. The results indicate that supplementation with live strain or with supernatant containing GABA produced by L. brevis BGZLS10-17 could alleviate the EAE symptoms. However, the use of L. brevis BGZLS10-17 in functional food as probiotic for autoimmune diseases should be tested in clinical trials.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis
EP  - 176
IS  - 2
SP  - 163
VL  - 85
DO  - 10.2298/JSC190716094S
ER  - 

@article{
author = "Soković Bajić, Svetlana and Mihajlović, Sanja B. and Radojević, Dušan and Popović, Dušanka and Đokić, Jelena and Stanisavljević, Suzana and Lazarević, Milica N. and Miljković, Djordje M. and Ruas-Madiedo, Patricia and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic autoimmune disease of the central nervous system. The aim of this work was to study the probiotic effect of gamma-aminobutyric acid (GABA)-producer Lactobacillus brevis BGZLS10-17 on experimental autoimmune encephalomyelitis (EAE), an experimental animal MS model. Clinical EAE symptoms were monitored in Dark Agouti (DA) rats treated with L. brevis BGZLS10-17 strain, or supernatant obtained from 48 h culture of L. brevis BGZLS10-17 cultivated in growth medium with or without GABA precursor monosodium glutamate (MSG). The results revealed that oral administration of L. brevis BGZLS10-17 alleviates the symptoms of EAE in DA rats. Namely, treatment with BGZLS10-17 and the supernatant of the strain cultivated in medium with MSG delayed the onset, shortened the duration, and reduced the intensity of the disease in the period when the EAE symptoms in controls were most pronounced. The probiotic treated animals were completely recovered after forty days, unlike the control animals. The results indicate that supplementation with live strain or with supernatant containing GABA produced by L. brevis BGZLS10-17 could alleviate the EAE symptoms. However, the use of L. brevis BGZLS10-17 in functional food as probiotic for autoimmune diseases should be tested in clinical trials.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis",
pages = "176-163",
number = "2",
volume = "85",
doi = "10.2298/JSC190716094S"
}

Soković Bajić, S., Mihajlović, S. B., Radojević, D., Popović, D., Đokić, J., Stanisavljević, S., Lazarević, M. N., Miljković, D. M., Ruas-Madiedo, P., Golić, N.,& Tolinački, M.. (2020). Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(2), 163-176.
https://doi.org/10.2298/JSC190716094S

Soković Bajić S, Mihajlović SB, Radojević D, Popović D, Đokić J, Stanisavljević S, Lazarević MN, Miljković DM, Ruas-Madiedo P, Golić N, Tolinački M. Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis. in Journal of the Serbian Chemical Society. 2020;85(2):163-176.
doi:10.2298/JSC190716094S .

Soković Bajić, Svetlana, Mihajlović, Sanja B., Radojević, Dušan, Popović, Dušanka, Đokić, Jelena, Stanisavljević, Suzana, Lazarević, Milica N., Miljković, Djordje M., Ruas-Madiedo, Patricia, Golić, Nataša, Tolinački, Maja, "Characterization of pH resistance and the proteolytic activity of GABA producing Lactobacillus brevis BGZLS10-17 in preparation of fermented milk beverage and the effects on the symptoms of the experimental autoimmune encephalomyelitis" in Journal of the Serbian Chemical Society, 85, no. 2 (2020):163-176,
https://doi.org/10.2298/JSC190716094S . .

TY  - JOUR
AU  - Đedović, Neda
AU  - Jevtić, Bojan
AU  - Jose Mansilla, M.
AU  - Petković, Filip
AU  - Blazevski, Jana
AU  - Timotijević, Gordana
AU  - Navarro-Barriuso, Juan
AU  - Martinez-Caceres, Eva
AU  - Mostarica Stojkovide, Marija
AU  - Miljković, Đorđe
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1252
AB  - Dendritic cells (DC) are responsible for the initiation and shaping of the adaptive immune response and are in the focus of autoimmunity research. We were interested in comparison of DC obtained from autoimmunity-prone Dark Agouti (DA) rats and autoimmunity-resistant Albino Oxford (AO) rats. DC were generated from bone marrow precursors and matured (mDC) by lipopolysaccharide. Tolerogenic DC (tolDC) obtained by vitamin D3 treatment were studied in parallel. Profile of cytokine production was different in AO and DA mDC and tolDC. Expression of MHC class II molecules and CD86 were higher in DA DC, while vitamin D3 reduced their expression in dendritic cells of both strains. Allogeneic proliferation of CD4(+) T cells was reduced by AO tolDC, but not with DA tolDC in comparison to respective mDC. Finally, expression of various genes identified as differentially expressed in human mDC and tolDC was also analyzed in AO and DA DC. Again, AO and DA DC differed in the expression of the analyzed genes. To conclude, AO and DA DC differ in production of cytokines, expression of antigen presentation-related molecules and in regulation of CD4(+) T proliferation. The difference is valuable for understanding the divergence of the strains in their susceptibility to autoimmunity.
PB  - Elsevier Gmbh, Munich
T2  - Immunobiology
T1  - Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats
EP  - 476
IS  - 3
SP  - 470
VL  - 224
DO  - 10.1016/j.imbio.2019.01.001
ER  - 

@article{
author = "Đedović, Neda and Jevtić, Bojan and Jose Mansilla, M. and Petković, Filip and Blazevski, Jana and Timotijević, Gordana and Navarro-Barriuso, Juan and Martinez-Caceres, Eva and Mostarica Stojkovide, Marija and Miljković, Đorđe",
year = "2019",
abstract = "Dendritic cells (DC) are responsible for the initiation and shaping of the adaptive immune response and are in the focus of autoimmunity research. We were interested in comparison of DC obtained from autoimmunity-prone Dark Agouti (DA) rats and autoimmunity-resistant Albino Oxford (AO) rats. DC were generated from bone marrow precursors and matured (mDC) by lipopolysaccharide. Tolerogenic DC (tolDC) obtained by vitamin D3 treatment were studied in parallel. Profile of cytokine production was different in AO and DA mDC and tolDC. Expression of MHC class II molecules and CD86 were higher in DA DC, while vitamin D3 reduced their expression in dendritic cells of both strains. Allogeneic proliferation of CD4(+) T cells was reduced by AO tolDC, but not with DA tolDC in comparison to respective mDC. Finally, expression of various genes identified as differentially expressed in human mDC and tolDC was also analyzed in AO and DA DC. Again, AO and DA DC differed in the expression of the analyzed genes. To conclude, AO and DA DC differ in production of cytokines, expression of antigen presentation-related molecules and in regulation of CD4(+) T proliferation. The difference is valuable for understanding the divergence of the strains in their susceptibility to autoimmunity.",
publisher = "Elsevier Gmbh, Munich",
journal = "Immunobiology",
title = "Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats",
pages = "476-470",
number = "3",
volume = "224",
doi = "10.1016/j.imbio.2019.01.001"
}

Đedović, N., Jevtić, B., Jose Mansilla, M., Petković, F., Blazevski, J., Timotijević, G., Navarro-Barriuso, J., Martinez-Caceres, E., Mostarica Stojkovide, M.,& Miljković, Đ.. (2019). Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats. in Immunobiology
Elsevier Gmbh, Munich., 224(3), 470-476.
https://doi.org/10.1016/j.imbio.2019.01.001

Đedović N, Jevtić B, Jose Mansilla M, Petković F, Blazevski J, Timotijević G, Navarro-Barriuso J, Martinez-Caceres E, Mostarica Stojkovide M, Miljković Đ. Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats. in Immunobiology. 2019;224(3):470-476.
doi:10.1016/j.imbio.2019.01.001 .

Đedović, Neda, Jevtić, Bojan, Jose Mansilla, M., Petković, Filip, Blazevski, Jana, Timotijević, Gordana, Navarro-Barriuso, Juan, Martinez-Caceres, Eva, Mostarica Stojkovide, Marija, Miljković, Đorđe, "Comparison of dendritic cells obtained from autoimmunty-prone and resistant rats" in Immunobiology, 224, no. 3 (2019):470-476,
https://doi.org/10.1016/j.imbio.2019.01.001 . .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Cepić, Aleksa
AU  - Bojić, Svetlana
AU  - Veljović, Katarina
AU  - Mihajlović, Sanja
AU  - Dedović, Neda
AU  - Jevtić, Bojan
AU  - Momcilović, Miljana
AU  - Lazarević, Milica
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Đorđe
AU  - Golić, Nataša
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1272
AB  - Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats
SP  - 918
VL  - 9
DO  - 10.1038/s41598-018-37505-7
ER  - 

@article{
author = "Stanisavljević, Suzana and Cepić, Aleksa and Bojić, Svetlana and Veljović, Katarina and Mihajlović, Sanja and Dedović, Neda and Jevtić, Bojan and Momcilović, Miljana and Lazarević, Milica and Mostarica-Stojković, Marija and Miljković, Đorđe and Golić, Nataša",
year = "2019",
abstract = "Gut microbiota dysbiosis has been considered the essential element in the pathogenesis of multiple sclerosis and its animal model, experimental autoimmune encephalomyelitis (EAE). Antibiotics were administered orally to Dark Agouti (DA) rats early in their life with the aim of perturbing gut microbiota and investigating the effects of such intervention on the course of EAE. As a result, the diversity of the gut microbiota was reduced under the influence of antibiotics. Mainly, Firmicutes and Actinobacteria were replaced by Proteobacteria and Bacteroidetes, while decreased proportions of Clostridia and Bacilli classes were accompanied by an increase in Gamma-Proteobacteria in antibiotic-treated animals. Interestingly, a notable decrease in the Helicobacteraceae, Spirochaetaceae and Turicibacteriaceae was scored in antibiotic-treated groups. Also, levels of short chain fatty acids were reduced in the faeces of antibiotic-treated rats. Consequently, aggravation of EAE, paralleled with stronger immune response in lymph nodes draining the site of immunization, and increased inflammation within the CNS, were observed in antibiotic-treated DA rats. Thus, the alteration of gut microbiota leads to an escalation of CNS-directed autoimmunity in DA rats. The results of this study indicate that antibiotic use in early life may have subsequent unfavourable effects on the regulation of the immune system.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats",
pages = "918",
volume = "9",
doi = "10.1038/s41598-018-37505-7"
}

Stanisavljević, S., Cepić, A., Bojić, S., Veljović, K., Mihajlović, S., Dedović, N., Jevtić, B., Momcilović, M., Lazarević, M., Mostarica-Stojković, M., Miljković, Đ.,& Golić, N.. (2019). Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats. in Scientific Reports
Nature Publishing Group, London., 9, 918.
https://doi.org/10.1038/s41598-018-37505-7

Stanisavljević S, Cepić A, Bojić S, Veljović K, Mihajlović S, Dedović N, Jevtić B, Momcilović M, Lazarević M, Mostarica-Stojković M, Miljković Đ, Golić N. Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats. in Scientific Reports. 2019;9:918.
doi:10.1038/s41598-018-37505-7 .

Stanisavljević, Suzana, Cepić, Aleksa, Bojić, Svetlana, Veljović, Katarina, Mihajlović, Sanja, Dedović, Neda, Jevtić, Bojan, Momcilović, Miljana, Lazarević, Milica, Mostarica-Stojković, Marija, Miljković, Đorđe, Golić, Nataša, "Oral neonatal antibiotic treatment perturbs gut microbiota and aggravates central nervous system autoimmunity in Dark Agouti rats" in Scientific Reports, 9 (2019):918,
https://doi.org/10.1038/s41598-018-37505-7 . .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Dinić, Miroslav
AU  - Jevtić, Bojan
AU  - Dedović, Neda
AU  - Momcilović, Miljana
AU  - Đokić, Jelena
AU  - Golić, Nataša
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Đorđe
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1179
AB  - Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-. and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Immunology
T1  - Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis
VL  - 9
DO  - 10.3389/fimmu.2018.00942
ER  - 

@article{
author = "Stanisavljević, Suzana and Dinić, Miroslav and Jevtić, Bojan and Dedović, Neda and Momcilović, Miljana and Đokić, Jelena and Golić, Nataša and Mostarica-Stojković, Marija and Miljković, Đorđe",
year = "2018",
abstract = "Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-. and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Immunology",
title = "Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis",
volume = "9",
doi = "10.3389/fimmu.2018.00942"
}

Stanisavljević, S., Dinić, M., Jevtić, B., Dedović, N., Momcilović, M., Đokić, J., Golić, N., Mostarica-Stojković, M.,& Miljković, Đ.. (2018). Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis. in Frontiers in Immunology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fimmu.2018.00942

Stanisavljević S, Dinić M, Jevtić B, Dedović N, Momcilović M, Đokić J, Golić N, Mostarica-Stojković M, Miljković Đ. Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis. in Frontiers in Immunology. 2018;9.
doi:10.3389/fimmu.2018.00942 .

Stanisavljević, Suzana, Dinić, Miroslav, Jevtić, Bojan, Dedović, Neda, Momcilović, Miljana, Đokić, Jelena, Golić, Nataša, Mostarica-Stojković, Marija, Miljković, Đorđe, "Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis" in Frontiers in Immunology, 9 (2018),
https://doi.org/10.3389/fimmu.2018.00942 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Đedović, Neda
AU  - Stanisavljević, Suzana
AU  - Gašić, Uroš
AU  - Misić, Danijela
AU  - Despotović, Jovana
AU  - Samardžić, Jelena
AU  - Miljković, Djordje
AU  - Timotijević, Gordana
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1000
AB  - Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Functional Foods
T1  - Anti-encephalitogenic effects of cucumber leaf extract
EP  - 262
SP  - 249
VL  - 37
DO  - 10.1016/j.jff.2017.07.060
ER  - 

@article{
author = "Jevtić, Bojan and Đedović, Neda and Stanisavljević, Suzana and Gašić, Uroš and Misić, Danijela and Despotović, Jovana and Samardžić, Jelena and Miljković, Djordje and Timotijević, Gordana",
year = "2017",
abstract = "Cucumber (Cucumis sativus) fruit has been used in cuisine worldwide, while its leaves are rich in immunomodulatory compounds. Cucumber leaf extract (CLE) was characterized by the predominance of triterpenoids cucurbitacins and significant levels of phenolics. Effects of CLE on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system were investigated in our study. CLE potently inhibited production of major pathogenic Th cytokines: interferon-gamma and interleukin-17, as well as of nitric oxide and reactive oxygen species in macrophages. Antigen-presenting activity of macrophages and dendritic cells was also affected by CLE. The effects of CLE were co-incident with modulation of NFKB and p38 MAPK signaling. Concentrations of CLE used in vitro did not show toxic effects on zebrafish embryos. Moreover, CLE inhibited generation of encephalitogenic cells in vivo. These results demonstrate that CLE deserve further investigation on its anti-encephalitogenic therapeutic properties.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Functional Foods",
title = "Anti-encephalitogenic effects of cucumber leaf extract",
pages = "262-249",
volume = "37",
doi = "10.1016/j.jff.2017.07.060"
}

Jevtić, B., Đedović, N., Stanisavljević, S., Gašić, U., Misić, D., Despotović, J., Samardžić, J., Miljković, D.,& Timotijević, G.. (2017). Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods
Elsevier Science Bv, Amsterdam., 37, 249-262.
https://doi.org/10.1016/j.jff.2017.07.060

Jevtić B, Đedović N, Stanisavljević S, Gašić U, Misić D, Despotović J, Samardžić J, Miljković D, Timotijević G. Anti-encephalitogenic effects of cucumber leaf extract. in Journal of Functional Foods. 2017;37:249-262.
doi:10.1016/j.jff.2017.07.060 .

Jevtić, Bojan, Đedović, Neda, Stanisavljević, Suzana, Gašić, Uroš, Misić, Danijela, Despotović, Jovana, Samardžić, Jelena, Miljković, Djordje, Timotijević, Gordana, "Anti-encephalitogenic effects of cucumber leaf extract" in Journal of Functional Foods, 37 (2017):249-262,
https://doi.org/10.1016/j.jff.2017.07.060 . .

TY  - JOUR
AU  - Stanisavljević, S.
AU  - Lukić, Jovanka
AU  - Momcilović, M.
AU  - Miljković, M.
AU  - Jevtić, B.
AU  - Kojić, Milan
AU  - Golić, Nataša
AU  - Mostarica Stojković, M.
AU  - Miljković, D.
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/947
AB  - Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis
EP  - 373
IS  - 3
SP  - 363
VL  - 7
DO  - 10.3920/BM2015.0159
ER  - 

@article{
author = "Stanisavljević, S. and Lukić, Jovanka and Momcilović, M. and Miljković, M. and Jevtić, B. and Kojić, Milan and Golić, Nataša and Mostarica Stojković, M. and Miljković, D.",
year = "2016",
abstract = "Gut microbiota and gut-associated lymphoid tissue have been increasingly appreciated as important players in pathogenesis of various autoimmune diseases, including multiple sclerosis. Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis that can be induced with an injection of spinal cord homogenate emulsified in complete Freund's adjuvant in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats. In this study, mesenteric lymph nodes (MLN), Peyer's patches (PP) and gut microbiota were analysed in these two rat strains. There was higher proportion of CD4(+) T cells and regulatory T cells in non-immunised DA rats in comparison to AO rats. Also, DA rat MLN and PP cells were higher producers of pro-inflammatory cytokines interferon-gamma and interleukin-17. Finally, microbial analyses showed that uncultivated species of Turicibacter and Atopostipes genus were exclusively present in AO rats, in faeces and intestinal tissue, respectively. Thus, it is clear that in comparison of an EAE-susceptible with an EAE-resistant strain of rats, various discrepancies at the level of gut associated lymphoid tissue, as well as at the level of gut microbiota can be observed. Future studies should determine if the differences have functional significance for EAE pathogenesis.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis",
pages = "373-363",
number = "3",
volume = "7",
doi = "10.3920/BM2015.0159"
}

Stanisavljević, S., Lukić, J., Momcilović, M., Miljković, M., Jevtić, B., Kojić, M., Golić, N., Mostarica Stojković, M.,& Miljković, D.. (2016). Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 7(3), 363-373.
https://doi.org/10.3920/BM2015.0159

Stanisavljević S, Lukić J, Momcilović M, Miljković M, Jevtić B, Kojić M, Golić N, Mostarica Stojković M, Miljković D. Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis. in Beneficial Microbes. 2016;7(3):363-373.
doi:10.3920/BM2015.0159 .

Stanisavljević, S., Lukić, Jovanka, Momcilović, M., Miljković, M., Jevtić, B., Kojić, Milan, Golić, Nataša, Mostarica Stojković, M., Miljković, D., "Gut-associated lymphoid tissue, gut microbes and susceptibility to experimental autoimmune encephalomyelitis" in Beneficial Microbes, 7, no. 3 (2016):363-373,
https://doi.org/10.3920/BM2015.0159 . .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Lukić, Jovanka
AU  - Soković Bajić, Svetlana
AU  - Mihajlović, Sanja
AU  - Mostarica Stojković, Marija
AU  - Miljković, Djordje
AU  - Golić, Nataša
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/950
AB  - Multiple sclerosis is a chronic inflammatory disease of the central nervous system (CNS). It is widely accepted that autoimmune response against the antigens of the CNS is the essential pathogenic force in the disease. It has recently become increasingly appreciated that activated encephalitogenic cells tend to migrate toward gut associated lymphoid tissues (GALTs) and that interrupted balance between regulatory and inflammatory immunity within the GALT might have decisive role in the initiation and propagation of the CNS autoimmunity. Gut microbiota composition and function has the major impact on the balance in the GALT. Thus, our aim was to perform analyses of gut microbiota in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Albino Oxford (AO) rats that are highly resistant to EAE induction and Dark Agouti (DA) rats that develop EAE after mild immunization were compared for gut microbiota composition in different phases after EAE induction. Microbial analyses of the genus Lactobacillus and related lactic acid bacteria showed higher diversity of Lactobacillus spp. in EAE-resistant AO rats, while some members of Firmicutes and Proteobactene (Undibacterium oligocarboniphilum) were detected only in feces of DA rats at the peak of the disease (between 13 and 16 days after induction). Interestingly, in contrast to our previous study where Turicibacter sp. was found exclusively in non immunized AO, but not in DA rats, in this study it was detected in DA rats that remained healthy 16 days after induction, as well as in four of 12 DA rats at the peak of the disease. Similar observation was obtained for the members of Lachnospiraceae. Further, production of a typical regulatory cytokine interleukin-10 was compared in GALT cells of AO and DA rats, and higher production was observed in DA rats. Our data contribute to the idea that gut microbiota and GALT considerably influence multiple sclerosis pathogenesis.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats
VL  - 7
DO  - 10.3389/fmicb.2016.02005
ER  - 

@article{
author = "Stanisavljević, Suzana and Lukić, Jovanka and Soković Bajić, Svetlana and Mihajlović, Sanja and Mostarica Stojković, Marija and Miljković, Djordje and Golić, Nataša",
year = "2016",
abstract = "Multiple sclerosis is a chronic inflammatory disease of the central nervous system (CNS). It is widely accepted that autoimmune response against the antigens of the CNS is the essential pathogenic force in the disease. It has recently become increasingly appreciated that activated encephalitogenic cells tend to migrate toward gut associated lymphoid tissues (GALTs) and that interrupted balance between regulatory and inflammatory immunity within the GALT might have decisive role in the initiation and propagation of the CNS autoimmunity. Gut microbiota composition and function has the major impact on the balance in the GALT. Thus, our aim was to perform analyses of gut microbiota in experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis. Albino Oxford (AO) rats that are highly resistant to EAE induction and Dark Agouti (DA) rats that develop EAE after mild immunization were compared for gut microbiota composition in different phases after EAE induction. Microbial analyses of the genus Lactobacillus and related lactic acid bacteria showed higher diversity of Lactobacillus spp. in EAE-resistant AO rats, while some members of Firmicutes and Proteobactene (Undibacterium oligocarboniphilum) were detected only in feces of DA rats at the peak of the disease (between 13 and 16 days after induction). Interestingly, in contrast to our previous study where Turicibacter sp. was found exclusively in non immunized AO, but not in DA rats, in this study it was detected in DA rats that remained healthy 16 days after induction, as well as in four of 12 DA rats at the peak of the disease. Similar observation was obtained for the members of Lachnospiraceae. Further, production of a typical regulatory cytokine interleukin-10 was compared in GALT cells of AO and DA rats, and higher production was observed in DA rats. Our data contribute to the idea that gut microbiota and GALT considerably influence multiple sclerosis pathogenesis.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats",
volume = "7",
doi = "10.3389/fmicb.2016.02005"
}

Stanisavljević, S., Lukić, J., Soković Bajić, S., Mihajlović, S., Mostarica Stojković, M., Miljković, D.,& Golić, N.. (2016). Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmicb.2016.02005

Stanisavljević S, Lukić J, Soković Bajić S, Mihajlović S, Mostarica Stojković M, Miljković D, Golić N. Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats. in Frontiers in Microbiology. 2016;7.
doi:10.3389/fmicb.2016.02005 .

Stanisavljević, Suzana, Lukić, Jovanka, Soković Bajić, Svetlana, Mihajlović, Sanja, Mostarica Stojković, Marija, Miljković, Djordje, Golić, Nataša, "Correlation of Gut Microbiota Composition with Resistance to Experimental Autoimmune Encephalomyelitis in Rats" in Frontiers in Microbiology, 7 (2016),
https://doi.org/10.3389/fmicb.2016.02005 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Đedović, Neda
AU  - Stanisavljević, Suzana
AU  - Despotović, Jovana
AU  - Mijković, Djordje
AU  - Timotijević, Gordana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/938
AB  - Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4+ T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, Na kappa B, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.
PB  - Amer Chemical Soc, Washington
T2  - Journal of Agricultural and Food Chemistry
T1  - Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells
EP  - 4907
IS  - 24
SP  - 4900
VL  - 64
DO  - 10.1021/acs.jafc.6b00951
ER  - 

@article{
author = "Jevtić, Bojan and Đedović, Neda and Stanisavljević, Suzana and Despotović, Jovana and Mijković, Djordje and Timotijević, Gordana",
year = "2016",
abstract = "Cucurbitacin E (CucE) is a highly oxidized steroid consisting of a tetracyclic triterpene. It is a member of a Cucurbitacin family of biomolecules that are predominantly found in Cucurbitaceae plants. CucE has already been identified as a potent anti-inflammatory compound. Here, its effects on CD4(+) T helper (Th) cells and macrophages, as the major encephalitogenic cells in the autoimmunity of the central nervous system, were investigated. Production of major pathogenic Th cell cytokines: interferon-gamma and interleukin-17 were inhibited under the influence of CucE. The effects of CucE on CD4+ T cells were mediated through the modulation of aryl hydrocarbon receptor, STAT3, Na kappa B, p38 MAPK, and miR-146 signaling. Further, production of nitric oxide and reactive oxygen species, as well as phagocytic ability, were inhibited in macrophages treated with CucE. These results imply that CucE possesses powerful antiencephalitogenic activity.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells",
pages = "4907-4900",
number = "24",
volume = "64",
doi = "10.1021/acs.jafc.6b00951"
}

Jevtić, B., Đedović, N., Stanisavljević, S., Despotović, J., Mijković, D.,& Timotijević, G.. (2016). Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 64(24), 4900-4907.
https://doi.org/10.1021/acs.jafc.6b00951

Jevtić B, Đedović N, Stanisavljević S, Despotović J, Mijković D, Timotijević G. Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells. in Journal of Agricultural and Food Chemistry. 2016;64(24):4900-4907.
doi:10.1021/acs.jafc.6b00951 .

Jevtić, Bojan, Đedović, Neda, Stanisavljević, Suzana, Despotović, Jovana, Mijković, Djordje, Timotijević, Gordana, "Cucurbitacin E Potently Modulates the Activity of Encephalitogenic Cells" in Journal of Agricultural and Food Chemistry, 64, no. 24 (2016):4900-4907,
https://doi.org/10.1021/acs.jafc.6b00951 . .

TY  - JOUR
AU  - Jevtić, Bojan
AU  - Timotijević, Gordana
AU  - Stanisavljević, Suzana
AU  - Momcilović, Miljana
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Djordje
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/821
AB  - MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation. As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Paris
T2  - Biomedicine & Pharmacotherapy
T1  - Micro RNA-155 participates in re-activation of encephalitogenic T cells
EP  - 210
SP  - 206
VL  - 74
DO  - 10.1016/j.biopha.2015.08.011
ER  - 

@article{
author = "Jevtić, Bojan and Timotijević, Gordana and Stanisavljević, Suzana and Momcilović, Miljana and Mostarica-Stojković, Marija and Miljković, Djordje",
year = "2015",
abstract = "MicroRNAs (miR) are small non-coding RNAs involved in the immune response regulation. miR-155 has been attributed a major pro-inflammatory role in the pathogenesis of multiple sclerosis and its animal model experimental autoimmune encephalomyelitis (EAE). Here, a role of miR-155 in re-activation of encephalitogenic CD4(+) T cells was investigated. Dark Agouti rats were immunized with myelin basic protein (MBP) emulsified in complete Freund's adjuvant. CD4(+) T cells were purified from draining lymph node cells (DLNC) obtained in the inductive phase and from spinal cord immune cells (SCIC) isolated at the peak of EAE. CD4(+) T cells obtained from SCIC (i.e., in vivo re-activated cells) had markedly higher expression of miR-155 in comparison to those purified from DLNC (not re-activated). Likewise, in vitro re-activation of DLNC with MBP led to increase in miR-155 expression. Further, DLNC and DLNC CD4(+) T cells were transfected with an inhibitor of miR-155 during in vitro re-activation. As a result, expression of important CD4(+) T cell effector cytokines IFN-gamma and IL-17, but not of regulatory cytokines IL-10 and TGF-beta, was reduced. These results imply that miR-155 supports re-activation of encephalitogenic CD4+ T cells. Our results contribute to a view that miR-155 might be a valuable target in multiple sclerosis therapy.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Paris",
journal = "Biomedicine & Pharmacotherapy",
title = "Micro RNA-155 participates in re-activation of encephalitogenic T cells",
pages = "210-206",
volume = "74",
doi = "10.1016/j.biopha.2015.08.011"
}

Jevtić, B., Timotijević, G., Stanisavljević, S., Momcilović, M., Mostarica-Stojković, M.,& Miljković, D.. (2015). Micro RNA-155 participates in re-activation of encephalitogenic T cells. in Biomedicine & Pharmacotherapy
Elsevier France-Editions Scientifiques Medicales Elsevier, Paris., 74, 206-210.
https://doi.org/10.1016/j.biopha.2015.08.011

Jevtić B, Timotijević G, Stanisavljević S, Momcilović M, Mostarica-Stojković M, Miljković D. Micro RNA-155 participates in re-activation of encephalitogenic T cells. in Biomedicine & Pharmacotherapy. 2015;74:206-210.
doi:10.1016/j.biopha.2015.08.011 .

Jevtić, Bojan, Timotijević, Gordana, Stanisavljević, Suzana, Momcilović, Miljana, Mostarica-Stojković, Marija, Miljković, Djordje, "Micro RNA-155 participates in re-activation of encephalitogenic T cells" in Biomedicine & Pharmacotherapy, 74 (2015):206-210,
https://doi.org/10.1016/j.biopha.2015.08.011 . .

TY  - JOUR
AU  - Petković, F.
AU  - Zivanović, J.
AU  - Blazevski, J.
AU  - Timotijević, Gordana
AU  - Momcilović, M.
AU  - Stanojević, Z.
AU  - Stamenković, V.
AU  - Milosević, V.
AU  - Mostarica-Stojković, Marija
AU  - Miljković, D.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/844
AB  - Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neuroscience
T1  - Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis
EP  - 12
SP  - 1
VL  - 292
DO  - 10.1016/j.neuroscience.2015.02.015
ER  - 

@article{
author = "Petković, F. and Zivanović, J. and Blazevski, J. and Timotijević, Gordana and Momcilović, M. and Stanojević, Z. and Stamenković, V. and Milosević, V. and Mostarica-Stojković, Marija and Miljković, D.",
year = "2015",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is a model of multiple sclerosis (MS), inflammatory, demyelinating and neurodegenerative disease of the central nervous system (CNS). Clinically manifested EAE can be induced in Dark Agouti (DA) rats, but not in Albino Oxford (AO) rats by immunization with spinal cord homogenate (SCH) and complete Freund's adjuvant (CFA). Matrix metalloproteinases (MMP) play important roles in various steps of MS and EAE pathogenesis. Expression of gelatinases MMP2 and MMP9, their activator MMP14 and their inhibitor tissue inhibitor of MMP (TIMP) 1 in the CNS of AO and DA rats immunized with SCH + CFA was determined. Expression of mRNA for MMP2, MMP9 and MMP14 was higher and expression of TIMP1 mRNA was lower in AO rats. However, gelatinase activity in spinal cords was higher in samples obtained from DA rats. Further, while there was no strain difference in MMP2 and MMP9 mRNA expression in lymph nodes of the immunized rats, gelatinase activity was higher in DA rats. This activity was reduced by antiinflammatory cytokines interleukin (IL)-10 and IL-4. Interestingly, gelatinase activity was detected in the nuclei of cells within the CNS, but not of those in lymph nodes. Our results imply that posttranscriptional regulation of MMP2 and MMP9 expression and/or function determines low gelatinase activity within the CNS and in immune cells of EAE-resistant AO rats.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neuroscience",
title = "Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis",
pages = "12-1",
volume = "292",
doi = "10.1016/j.neuroscience.2015.02.015"
}

Petković, F., Zivanović, J., Blazevski, J., Timotijević, G., Momcilović, M., Stanojević, Z., Stamenković, V., Milosević, V., Mostarica-Stojković, M.,& Miljković, D.. (2015). Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis. in Neuroscience
Pergamon-Elsevier Science Ltd, Oxford., 292, 1-12.
https://doi.org/10.1016/j.neuroscience.2015.02.015

Petković F, Zivanović J, Blazevski J, Timotijević G, Momcilović M, Stanojević Z, Stamenković V, Milosević V, Mostarica-Stojković M, Miljković D. Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis. in Neuroscience. 2015;292:1-12.
doi:10.1016/j.neuroscience.2015.02.015 .

Petković, F., Zivanović, J., Blazevski, J., Timotijević, Gordana, Momcilović, M., Stanojević, Z., Stamenković, V., Milosević, V., Mostarica-Stojković, Marija, Miljković, D., "Activity, but not MRNA expression of gelatinases correlates with susceptibility to experimental autoimmune encephalomyelitis" in Neuroscience, 292 (2015):1-12,
https://doi.org/10.1016/j.neuroscience.2015.02.015 . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Petković, Filip
AU  - Blazevski, Jana
AU  - Momcilović, Miljana
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Djordje
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/654
AB  - CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-gamma expression in neuroinflammation.
PB  - Elsevier Science Bv, Amsterdam
T2  - Brain Research
T1  - CXCL12-gamma expression is inhibited in neuroinflammation
EP  - 126
SP  - 120
VL  - 1519
DO  - 10.1016/j.brainres.2013.04.056
ER  - 

@article{
author = "Timotijević, Gordana and Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Mostarica-Stojković, Marija and Miljković, Djordje",
year = "2013",
abstract = "CXCL12 plays a protective role in CNS autoimmunity. Expression of CXCL12-gamma, which has distinct structural and functional properties than the other isoforms of CXCL12, was determined in spinal cords of rats immunized to develop experimental autoimmune encephalomyelitis (EAE). CNS expression of CXCL12-gamma was markedly lower in EAE-prone Dark Agouti rats than in EAE-resistant Albino Oxford rats, both in spinal cord homogenates and micro-blood vessels isolated from spinal cords. Inhibition of nitric oxide (NO) synthesis in DA rats upregulated, while donation of NO in AO rats downregulated CNS expression of CXCL12-gamma. NO inhibited CXCL12-gamma expression in astrocytes in vitro. A splice variant of CXCL12-gamma which migrates into nucleolus was not detected in spinal cord or astrocytes. Thus, CXCL12-gamma is expressed in the CNS after EAE induction, but its expression is markedly suppressed in spinal cord affected with full blown inflammation. NO is an important regulator of CXCL12-gamma expression in neuroinflammation.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Brain Research",
title = "CXCL12-gamma expression is inhibited in neuroinflammation",
pages = "126-120",
volume = "1519",
doi = "10.1016/j.brainres.2013.04.056"
}

Timotijević, G., Petković, F., Blazevski, J., Momcilović, M., Mostarica-Stojković, M.,& Miljković, D.. (2013). CXCL12-gamma expression is inhibited in neuroinflammation. in Brain Research
Elsevier Science Bv, Amsterdam., 1519, 120-126.
https://doi.org/10.1016/j.brainres.2013.04.056

Timotijević G, Petković F, Blazevski J, Momcilović M, Mostarica-Stojković M, Miljković D. CXCL12-gamma expression is inhibited in neuroinflammation. in Brain Research. 2013;1519:120-126.
doi:10.1016/j.brainres.2013.04.056 .

Timotijević, Gordana, Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Mostarica-Stojković, Marija, Miljković, Djordje, "CXCL12-gamma expression is inhibited in neuroinflammation" in Brain Research, 1519 (2013):120-126,
https://doi.org/10.1016/j.brainres.2013.04.056 . .

TY  - JOUR
AU  - Petković, Filip
AU  - Blazevski, Jana
AU  - Momcilović, Miljana
AU  - Timotijević, Gordana
AU  - Zocca, Mai-Britt
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Mangano, Katia
AU  - Fagone, Paolo
AU  - Stošić-Grujičić, Stanislava
AU  - Nicoletti, Ferdinando
AU  - Miljković, Djordje
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/647
AB  - NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases.
PB  - Elsevier Science Bv, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis
EP  - 65
IS  - 1-2
SP  - 55
VL  - 259
DO  - 10.1016/j.jneuroim.2013.03.010
ER  - 

@article{
author = "Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Timotijević, Gordana and Zocca, Mai-Britt and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Mangano, Katia and Fagone, Paolo and Stošić-Grujičić, Stanislava and Nicoletti, Ferdinando and Miljković, Djordje",
year = "2013",
abstract = "NO-hybridization of the HIV protease inhibitor Saquinavir generates a new chemical entity named Saq-NO, that retains the anti-viral activity and exerts lower toxicity. We show that Saq-NO inhibited the generation of various cytokines in ConA-stimulated unfractionated murine spleen cells and rat lymph nodes stimulated with ConA as well as in purified CD4(+) T cells in vitro and reduced the circulating levels of cytokines in mice challenged with anti-CD3 antibody. Furthermore, Saq-NO reduced IL-17 and IFN-gamma production in myelin basic protein (MBP)-specific cells isolated from rats immunized with MBP. These findings translated well into the in vivo setting as Saq-NO ameliorated the course of the disease in two preclinical models of multiple sclerosis. Our results demonstrate that Saq-NO exerts immunomodulatory effects that warrant studies on its application in autoimmune diseases.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis",
pages = "65-55",
number = "1-2",
volume = "259",
doi = "10.1016/j.jneuroim.2013.03.010"
}

Petković, F., Blazevski, J., Momcilović, M., Timotijević, G., Zocca, M., Mijatović, S., Maksimović-Ivanić, D., Mangano, K., Fagone, P., Stošić-Grujičić, S., Nicoletti, F.,& Miljković, D.. (2013). Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 259(1-2), 55-65.
https://doi.org/10.1016/j.jneuroim.2013.03.010

Petković F, Blazevski J, Momcilović M, Timotijević G, Zocca M, Mijatović S, Maksimović-Ivanić D, Mangano K, Fagone P, Stošić-Grujičić S, Nicoletti F, Miljković D. Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology. 2013;259(1-2):55-65.
doi:10.1016/j.jneuroim.2013.03.010 .

Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Timotijević, Gordana, Zocca, Mai-Britt, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Mangano, Katia, Fagone, Paolo, Stošić-Grujičić, Stanislava, Nicoletti, Ferdinando, Miljković, Djordje, "Saquinavir-NO inhibits S6 kinase activity, impairs secretion of the encephalytogenic cytokines interleukin-17 and interferon-gamma and ameliorates experimental autoimmune encephalomyelitis" in Journal of Neuroimmunology, 259, no. 1-2 (2013):55-65,
https://doi.org/10.1016/j.jneuroim.2013.03.010 . .

TY  - JOUR
AU  - Saksida, Tamara
AU  - Miljković, Djordje
AU  - Timotijević, Gordana
AU  - Stojanović, Ivana
AU  - Mijatović, Sanja
AU  - Fagone, Paolo
AU  - Mangano, Katia
AU  - Mammana, Santa
AU  - Farina, Claudio
AU  - Ascione, Ester
AU  - Maiello, Valentina
AU  - Nicoletti, Ferdinando
AU  - Stošić-Grujičić, Stanislava
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/627
AB  - Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1 beta, TNF, INF-gamma, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3(+) T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NF kappa B signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis.
PB  - Elsevier, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis
EP  - 78
IS  - 1-2
SP  - 72
VL  - 262
DO  - 10.1016/j.jneuroim.2013.07.001
ER  - 

@article{
author = "Saksida, Tamara and Miljković, Djordje and Timotijević, Gordana and Stojanović, Ivana and Mijatović, Sanja and Fagone, Paolo and Mangano, Katia and Mammana, Santa and Farina, Claudio and Ascione, Ester and Maiello, Valentina and Nicoletti, Ferdinando and Stošić-Grujičić, Stanislava",
year = "2013",
abstract = "Transferrin (Tf) has a major role in T cell activation and proliferation. Here, we investigated whether Tf exerts immunomodulatory effects on T cells and in development of T-cell driven experimental autoimmune encephalomyelitis (EAE). While treatment of concanavalin A-stimulated splenocytes with apotransferrin (ApoTf) did not affect release of IL-1 beta, TNF, INF-gamma, IL-17, IL-4, and IL-10, it markedly and dose-dependently down-regulated synthesis of IL-2 in these cells. ApoTf also inhibited IL-2 generation in purified CD3(+) T cells and the effect was accompanied with down-regulation of MAPK p44/42 and NF kappa B signaling. Despite impeded IL-2 release, proliferation of splenocytes was not inhibited by ApoTf. Importantly, ApoTf ameliorated EAE in mice and significantly reduced ex vivo IL-2 production in proteolipid protein-specific lymphocytes. Thus ApoTf may be a promising beneficial agent for multiple sclerosis.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis",
pages = "78-72",
number = "1-2",
volume = "262",
doi = "10.1016/j.jneuroim.2013.07.001"
}

Saksida, T., Miljković, D., Timotijević, G., Stojanović, I., Mijatović, S., Fagone, P., Mangano, K., Mammana, S., Farina, C., Ascione, E., Maiello, V., Nicoletti, F.,& Stošić-Grujičić, S.. (2013). Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology
Elsevier, Amsterdam., 262(1-2), 72-78.
https://doi.org/10.1016/j.jneuroim.2013.07.001

Saksida T, Miljković D, Timotijević G, Stojanović I, Mijatović S, Fagone P, Mangano K, Mammana S, Farina C, Ascione E, Maiello V, Nicoletti F, Stošić-Grujičić S. Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis. in Journal of Neuroimmunology. 2013;262(1-2):72-78.
doi:10.1016/j.jneuroim.2013.07.001 .

Saksida, Tamara, Miljković, Djordje, Timotijević, Gordana, Stojanović, Ivana, Mijatović, Sanja, Fagone, Paolo, Mangano, Katia, Mammana, Santa, Farina, Claudio, Ascione, Ester, Maiello, Valentina, Nicoletti, Ferdinando, Stošić-Grujičić, Stanislava, "Apotransferrin inhibits interleukin-2 expression and protects mice from experimental autoimmune encephalomyelitis" in Journal of Neuroimmunology, 262, no. 1-2 (2013):72-78,
https://doi.org/10.1016/j.jneuroim.2013.07.001 . .

TY  - JOUR
AU  - Đorđević, Valentina
AU  - Timotijević, Gordana
AU  - Pruner, Iva
AU  - Radojković, Dragica
AU  - Milovanović, Boško
AU  - Miljković, Đorđe
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/665
AB  - Uvod: Nosioci alela CCR5Δ32 su relativno rezistentni na infekciju HIV-om. Postoji nekoliko hipoteza o poreklu i održanju ovog alela u ljudskoj populaciji. Pretpostavlja se da je mutacija Δ32 nastala u populaciji severne ili istočne Evrope i da se potom proširila ka jugu. Iako je učestalost CCR5Δ32 određena u mnogim evropskim populacijama, dodatni podaci su neophodni za formiranje sveobuhvatne slike o distribuciji CCR5Δ32 u Evropi. Zbog toga smo u našoj studiji odredili učestalost CCR5Δ32 u srpskoj populaciji, za koju do ovog rada nisu postojali takvi podaci. Metode: DNK je izolovana iz periferne krvi 352 osobe. U reakciji lančanog umnožavanja korišćeni su prajmeri specifični za gen CCR5. Dobijen je proizvod od 263 bp na osnovu matrice 'wild type', sekvence CCR5 gena, a proizvod od 231 bp na osnovu okrnjene sekvence gena CCR5 (CCR5Δ32). Ukupna učestalost alela CCR5Δ32 u srpskoj populaciji iznosi 4,55%. Rezultati: Od ukupnog broja analiziranih osoba, identifikovano je 8,52% heterozigotnih i 0,57% homozigotnih nosilaca za ovaj alel. Zaključak: Utvrđena učestalost alela CCR5Δ32 u srpskoj populaciji je neočekivano niska, u poređenju sa učestalošću u ostalim slovenskim populacijama.
AB  - Background: The mutant CCR5Δ32 allele confers resistance to HIV infection. Several hypotheses regarding its origin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was introduced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was determined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 distribution within the continent. Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild­type CCR5 sequence and a product of 231 bp was obtained from the truncated CCR5Δ32 sequence. Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homozygous and 8.52% are heterozygous for CCR5Δ32. Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, considering ethnically related populations.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Učestalost alela CCR5Δ32 u srpskoj populaciji
T1  - The frequency of allele CCR5Δ32 in a Serbian population
EP  - 374
IS  - 4
SP  - 368
VL  - 32
DO  - 10.2478/jomb-2013-0030
ER  - 

@article{
author = "Đorđević, Valentina and Timotijević, Gordana and Pruner, Iva and Radojković, Dragica and Milovanović, Boško and Miljković, Đorđe",
year = "2013",
abstract = "Uvod: Nosioci alela CCR5Δ32 su relativno rezistentni na infekciju HIV-om. Postoji nekoliko hipoteza o poreklu i održanju ovog alela u ljudskoj populaciji. Pretpostavlja se da je mutacija Δ32 nastala u populaciji severne ili istočne Evrope i da se potom proširila ka jugu. Iako je učestalost CCR5Δ32 određena u mnogim evropskim populacijama, dodatni podaci su neophodni za formiranje sveobuhvatne slike o distribuciji CCR5Δ32 u Evropi. Zbog toga smo u našoj studiji odredili učestalost CCR5Δ32 u srpskoj populaciji, za koju do ovog rada nisu postojali takvi podaci. Metode: DNK je izolovana iz periferne krvi 352 osobe. U reakciji lančanog umnožavanja korišćeni su prajmeri specifični za gen CCR5. Dobijen je proizvod od 263 bp na osnovu matrice 'wild type', sekvence CCR5 gena, a proizvod od 231 bp na osnovu okrnjene sekvence gena CCR5 (CCR5Δ32). Ukupna učestalost alela CCR5Δ32 u srpskoj populaciji iznosi 4,55%. Rezultati: Od ukupnog broja analiziranih osoba, identifikovano je 8,52% heterozigotnih i 0,57% homozigotnih nosilaca za ovaj alel. Zaključak: Utvrđena učestalost alela CCR5Δ32 u srpskoj populaciji je neočekivano niska, u poređenju sa učestalošću u ostalim slovenskim populacijama., Background: The mutant CCR5Δ32 allele confers resistance to HIV infection. Several hypotheses regarding its origin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was introduced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was determined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 distribution within the continent. Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild­type CCR5 sequence and a product of 231 bp was obtained from the truncated CCR5Δ32 sequence. Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homozygous and 8.52% are heterozygous for CCR5Δ32. Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, considering ethnically related populations.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Učestalost alela CCR5Δ32 u srpskoj populaciji, The frequency of allele CCR5Δ32 in a Serbian population",
pages = "374-368",
number = "4",
volume = "32",
doi = "10.2478/jomb-2013-0030"
}

Đorđević, V., Timotijević, G., Pruner, I., Radojković, D., Milovanović, B.,& Miljković, Đ.. (2013). Učestalost alela CCR5Δ32 u srpskoj populaciji. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 32(4), 368-374.
https://doi.org/10.2478/jomb-2013-0030

Đorđević V, Timotijević G, Pruner I, Radojković D, Milovanović B, Miljković Đ. Učestalost alela CCR5Δ32 u srpskoj populaciji. in Journal of Medical Biochemistry. 2013;32(4):368-374.
doi:10.2478/jomb-2013-0030 .

Đorđević, Valentina, Timotijević, Gordana, Pruner, Iva, Radojković, Dragica, Milovanović, Boško, Miljković, Đorđe, "Učestalost alela CCR5Δ32 u srpskoj populaciji" in Journal of Medical Biochemistry, 32, no. 4 (2013):368-374,
https://doi.org/10.2478/jomb-2013-0030 . .

TY  - JOUR
AU  - Timotijević, Gordana
AU  - Mostarica-Stojković, Marija
AU  - Miljković, Djordje
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/558
AB  - CxCL12, also known as SDF-1 (stromal cell derived factor-1) is a small protein that belongs to the chemokine family, whose members have a crucial role in directing cell migration. CXCL12 has an essential role in neural and vascular development, hematopoiesis and in immunity. It acts through two receptors. CXCR4 and CXCR7. While the former is a classic G protein-coupled transmembrane chemokine receptor, the latter primarily function as a scavenger of CXCL12. CXCL12 has been considered as a standard proinflammatory molecule for a long time, as it attracts leukocytes to inflammatory sites and contributes to their activation. However, recent findings indicate that this chemokine has the opposite role in neuroinflammation. In this review, basic data about molecular and functional properties of CXCL12 are presented, while its role in CNS autoimmunity is addressed in details.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - International Journal of Biochemistry & Cell Biology
T1  - CXCL12: Role in neuroinflammation
EP  - 841
IS  - 6
SP  - 838
VL  - 44
DO  - 10.1016/j.biocel.2012.03.014
ER  - 

@article{
author = "Timotijević, Gordana and Mostarica-Stojković, Marija and Miljković, Djordje",
year = "2012",
abstract = "CxCL12, also known as SDF-1 (stromal cell derived factor-1) is a small protein that belongs to the chemokine family, whose members have a crucial role in directing cell migration. CXCL12 has an essential role in neural and vascular development, hematopoiesis and in immunity. It acts through two receptors. CXCR4 and CXCR7. While the former is a classic G protein-coupled transmembrane chemokine receptor, the latter primarily function as a scavenger of CXCL12. CXCL12 has been considered as a standard proinflammatory molecule for a long time, as it attracts leukocytes to inflammatory sites and contributes to their activation. However, recent findings indicate that this chemokine has the opposite role in neuroinflammation. In this review, basic data about molecular and functional properties of CXCL12 are presented, while its role in CNS autoimmunity is addressed in details.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "International Journal of Biochemistry & Cell Biology",
title = "CXCL12: Role in neuroinflammation",
pages = "841-838",
number = "6",
volume = "44",
doi = "10.1016/j.biocel.2012.03.014"
}

Timotijević, G., Mostarica-Stojković, M.,& Miljković, D.. (2012). CXCL12: Role in neuroinflammation. in International Journal of Biochemistry & Cell Biology
Pergamon-Elsevier Science Ltd, Oxford., 44(6), 838-841.
https://doi.org/10.1016/j.biocel.2012.03.014

Timotijević G, Mostarica-Stojković M, Miljković D. CXCL12: Role in neuroinflammation. in International Journal of Biochemistry & Cell Biology. 2012;44(6):838-841.
doi:10.1016/j.biocel.2012.03.014 .

Timotijević, Gordana, Mostarica-Stojković, Marija, Miljković, Djordje, "CXCL12: Role in neuroinflammation" in International Journal of Biochemistry & Cell Biology, 44, no. 6 (2012):838-841,
https://doi.org/10.1016/j.biocel.2012.03.014 . .

TY  - JOUR
AU  - Belij, Sandra
AU  - Miljković, Djordje
AU  - Popov, Aleksandra
AU  - Subota, Vesna
AU  - Timotijević, Gordana
AU  - Slavić, Marija
AU  - Mirkov, Ivana
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/545
AB  - Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity. Priming effect for respiratory burst was noted at both warfarin doses, while only high warfarin dose resulted in priming for adhesion, the rise in intracellular myeloperoxidase content/release and stimulation of nitric oxide production. Differential effects of high warfarin dose were noted on granulocyte cytokines IL-6 (lack of the effect), TNF-alpha (decreased release and mRNA expression) and IL-12 (increase in mRNA for IL-12 subunits p35 and p40). Changes in granulocytes seems not to rely on mitogen activated kinases p38 and ERK. Warfarin intake was associated with an increase in circulating IL-6, fibrinogen and haptoglobin and with changes in the activity of erythrocyte antioxidant enzymes superoxide dismutase and catalase. The effects of oral warfarin intake on peripheral blood granulocytes demonstrated in this study might be relevant for oral anticoagulant therapy strategies in humans.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats
EP  - 1507
IS  - 5
SP  - 1499
VL  - 50
DO  - 10.1016/j.fct.2012.01.049
ER  - 

@article{
author = "Belij, Sandra and Miljković, Djordje and Popov, Aleksandra and Subota, Vesna and Timotijević, Gordana and Slavić, Marija and Mirkov, Ivana and Kataranovski, Dragan and Kataranovski, Milena",
year = "2012",
abstract = "Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity. Priming effect for respiratory burst was noted at both warfarin doses, while only high warfarin dose resulted in priming for adhesion, the rise in intracellular myeloperoxidase content/release and stimulation of nitric oxide production. Differential effects of high warfarin dose were noted on granulocyte cytokines IL-6 (lack of the effect), TNF-alpha (decreased release and mRNA expression) and IL-12 (increase in mRNA for IL-12 subunits p35 and p40). Changes in granulocytes seems not to rely on mitogen activated kinases p38 and ERK. Warfarin intake was associated with an increase in circulating IL-6, fibrinogen and haptoglobin and with changes in the activity of erythrocyte antioxidant enzymes superoxide dismutase and catalase. The effects of oral warfarin intake on peripheral blood granulocytes demonstrated in this study might be relevant for oral anticoagulant therapy strategies in humans.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats",
pages = "1507-1499",
number = "5",
volume = "50",
doi = "10.1016/j.fct.2012.01.049"
}

Belij, S., Miljković, D., Popov, A., Subota, V., Timotijević, G., Slavić, M., Mirkov, I., Kataranovski, D.,& Kataranovski, M.. (2012). Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 50(5), 1499-1507.
https://doi.org/10.1016/j.fct.2012.01.049

Belij S, Miljković D, Popov A, Subota V, Timotijević G, Slavić M, Mirkov I, Kataranovski D, Kataranovski M. Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats. in Food and Chemical Toxicology. 2012;50(5):1499-1507.
doi:10.1016/j.fct.2012.01.049 .

Belij, Sandra, Miljković, Djordje, Popov, Aleksandra, Subota, Vesna, Timotijević, Gordana, Slavić, Marija, Mirkov, Ivana, Kataranovski, Dragan, Kataranovski, Milena, "Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats" in Food and Chemical Toxicology, 50, no. 5 (2012):1499-1507,
https://doi.org/10.1016/j.fct.2012.01.049 . .

TY  - JOUR
AU  - Miljković, Djordje
AU  - Timotijević, Gordana
AU  - Mostarica-Stojković, Marija
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/491
AB  - Astrocytes are the most abundant cell population within the CNS of mammals. Their glial role is perfectly performed in the healthy CNS as they support functions of neurons. The omnipresence of astrocytes throughout the white and grey matter and their intimate relation with blood vessels of the CNS, as well as numerous immunity-related actions that these cells are capable of, imply that astrocytes should have a prominent role in neuroinflammatory disorders, such as multiple sclerosis (MS). The role of astrocytes in MS is rather ambiguous, as they have the capacity to both stimulate and restrain neuroinflammation and tissue destruction. In this paper we present some of the proved and the proposed functions of astrocytes in neuroinflammation and discuss the effect of MS therapeutics on astrocytes.
PB  - Wiley, Hoboken
T2  - FEBS Letters
T1  - Astrocytes in the tempest of multiple sclerosis
EP  - 3788
IS  - 23
SP  - 3781
VL  - 585
DO  - 10.1016/j.febslet.2011.03.047
ER  - 

@article{
author = "Miljković, Djordje and Timotijević, Gordana and Mostarica-Stojković, Marija",
year = "2011",
abstract = "Astrocytes are the most abundant cell population within the CNS of mammals. Their glial role is perfectly performed in the healthy CNS as they support functions of neurons. The omnipresence of astrocytes throughout the white and grey matter and their intimate relation with blood vessels of the CNS, as well as numerous immunity-related actions that these cells are capable of, imply that astrocytes should have a prominent role in neuroinflammatory disorders, such as multiple sclerosis (MS). The role of astrocytes in MS is rather ambiguous, as they have the capacity to both stimulate and restrain neuroinflammation and tissue destruction. In this paper we present some of the proved and the proposed functions of astrocytes in neuroinflammation and discuss the effect of MS therapeutics on astrocytes.",
publisher = "Wiley, Hoboken",
journal = "FEBS Letters",
title = "Astrocytes in the tempest of multiple sclerosis",
pages = "3788-3781",
number = "23",
volume = "585",
doi = "10.1016/j.febslet.2011.03.047"
}

Miljković, D., Timotijević, G.,& Mostarica-Stojković, M.. (2011). Astrocytes in the tempest of multiple sclerosis. in FEBS Letters
Wiley, Hoboken., 585(23), 3781-3788.
https://doi.org/10.1016/j.febslet.2011.03.047

Miljković D, Timotijević G, Mostarica-Stojković M. Astrocytes in the tempest of multiple sclerosis. in FEBS Letters. 2011;585(23):3781-3788.
doi:10.1016/j.febslet.2011.03.047 .

Miljković, Djordje, Timotijević, Gordana, Mostarica-Stojković, Marija, "Astrocytes in the tempest of multiple sclerosis" in FEBS Letters, 585, no. 23 (2011):3781-3788,
https://doi.org/10.1016/j.febslet.2011.03.047 . .