TY  - DATA
AU  - Spasovski, Vesna
AU  - Srzentić Dražilov, Sanja
AU  - Nikčević, Gordana
AU  - Baščarević, Zoran
AU  - Stojiljković, Maja
AU  - Pavlović, Sonja
AU  - Spasovski, Duško
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2089
AB  - Background: Perthes disease is a juvenile form of osteonecrosis of the femoral head that affects children under the age of 15. One hundred years after its discovery, some light has been shed on its etiology and the biological factors relevant to its etiology and disease severity. Methods: The aim of this study was to summarize the literature findings on the biological factors relevant to the pathogenesis of Perthes disease, their diagnostic and clinical significance, and their therapeutic potential. A special focus on candidate genes as susceptibility factors and factors relevant to clinical severity was made, where studies reporting clinical or preclinical results were considered as the inclusion criteria. PubMed databases were searched by two independent researchers. Sixty-eight articles were included in this review. Results on the factors relevant to vascular involvement and inflammatory molecules indicated as factors that contribute to impaired bone remodeling have been summarized. Moreover, several candidate genes relevant to an active phase of the disease have been suggested as possible biological therapeutic targets. Conclusions: Delineation of molecular biomarkers that underlie the pathophysiological process of Perthes disease can allow for the provision of earlier and more accurate diagnoses of the disease and more precise follow-ups and treatment in the early phases of the disease.
PB  - MDPI
T2  - Diagnostics
T1  - Supplementary material for: Spasovski, V.; Srzentić Dražilov, S.; Nikčević, G.; Baščarević, Z.; Stojiljković, M.; Pavlović, S.; Spasovski, D. Molecular Biomarkers in Perthes Disease: A Review. Diagnostics 2023, 13 (3), 471. https://doi.org/10.3390/diagnostics13030471.
IS  - 3
SP  - 471
VL  - 13
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2089
ER  - 

@misc{
author = "Spasovski, Vesna and Srzentić Dražilov, Sanja and Nikčević, Gordana and Baščarević, Zoran and Stojiljković, Maja and Pavlović, Sonja and Spasovski, Duško",
year = "2023",
abstract = "Background: Perthes disease is a juvenile form of osteonecrosis of the femoral head that affects children under the age of 15. One hundred years after its discovery, some light has been shed on its etiology and the biological factors relevant to its etiology and disease severity. Methods: The aim of this study was to summarize the literature findings on the biological factors relevant to the pathogenesis of Perthes disease, their diagnostic and clinical significance, and their therapeutic potential. A special focus on candidate genes as susceptibility factors and factors relevant to clinical severity was made, where studies reporting clinical or preclinical results were considered as the inclusion criteria. PubMed databases were searched by two independent researchers. Sixty-eight articles were included in this review. Results on the factors relevant to vascular involvement and inflammatory molecules indicated as factors that contribute to impaired bone remodeling have been summarized. Moreover, several candidate genes relevant to an active phase of the disease have been suggested as possible biological therapeutic targets. Conclusions: Delineation of molecular biomarkers that underlie the pathophysiological process of Perthes disease can allow for the provision of earlier and more accurate diagnoses of the disease and more precise follow-ups and treatment in the early phases of the disease.",
publisher = "MDPI",
journal = "Diagnostics",
title = "Supplementary material for: Spasovski, V.; Srzentić Dražilov, S.; Nikčević, G.; Baščarević, Z.; Stojiljković, M.; Pavlović, S.; Spasovski, D. Molecular Biomarkers in Perthes Disease: A Review. Diagnostics 2023, 13 (3), 471. https://doi.org/10.3390/diagnostics13030471.",
number = "3",
pages = "471",
volume = "13",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2089"
}

Spasovski, V., Srzentić Dražilov, S., Nikčević, G., Baščarević, Z., Stojiljković, M., Pavlović, S.,& Spasovski, D.. (2023). Supplementary material for: Spasovski, V.; Srzentić Dražilov, S.; Nikčević, G.; Baščarević, Z.; Stojiljković, M.; Pavlović, S.; Spasovski, D. Molecular Biomarkers in Perthes Disease: A Review. Diagnostics 2023, 13 (3), 471. https://doi.org/10.3390/diagnostics13030471.. in Diagnostics
MDPI., 13(3), 471.
https://hdl.handle.net/21.15107/rcub_imagine_2089

Spasovski V, Srzentić Dražilov S, Nikčević G, Baščarević Z, Stojiljković M, Pavlović S, Spasovski D. Supplementary material for: Spasovski, V.; Srzentić Dražilov, S.; Nikčević, G.; Baščarević, Z.; Stojiljković, M.; Pavlović, S.; Spasovski, D. Molecular Biomarkers in Perthes Disease: A Review. Diagnostics 2023, 13 (3), 471. https://doi.org/10.3390/diagnostics13030471.. in Diagnostics. 2023;13(3):471.
https://hdl.handle.net/21.15107/rcub_imagine_2089 .

Spasovski, Vesna, Srzentić Dražilov, Sanja, Nikčević, Gordana, Baščarević, Zoran, Stojiljković, Maja, Pavlović, Sonja, Spasovski, Duško, "Supplementary material for: Spasovski, V.; Srzentić Dražilov, S.; Nikčević, G.; Baščarević, Z.; Stojiljković, M.; Pavlović, S.; Spasovski, D. Molecular Biomarkers in Perthes Disease: A Review. Diagnostics 2023, 13 (3), 471. https://doi.org/10.3390/diagnostics13030471." in Diagnostics, 13, no. 3 (2023):471,
https://hdl.handle.net/21.15107/rcub_imagine_2089 .

TY  - JOUR
AU  - Spasovski, Vesna
AU  - Srzentić Dražilov, Sanja
AU  - Nikčević, Gordana
AU  - Baščarević, Zoran
AU  - Stojiljković, Maja
AU  - Pavlović, Sonja
AU  - Spasovski, Duško
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2088
AB  - Background: Perthes disease is a juvenile form of osteonecrosis of the femoral head that affects children under the age of 15. One hundred years after its discovery, some light has been shed on its etiology and the biological factors relevant to its etiology and disease severity. Methods: The aim of this study was to summarize the literature findings on the biological factors relevant to the pathogenesis of Perthes disease, their diagnostic and clinical significance, and their therapeutic potential. A special focus on candidate genes as susceptibility factors and factors relevant to clinical severity was made, where studies reporting clinical or preclinical results were considered as the inclusion criteria. PubMed databases were searched by two independent researchers. Sixty-eight articles were included in this review. Results on the factors relevant to vascular involvement and inflammatory molecules indicated as factors that contribute to impaired bone remodeling have been summarized. Moreover, several candidate genes relevant to an active phase of the disease have been suggested as possible biological therapeutic targets. Conclusions: Delineation of molecular biomarkers that underlie the pathophysiological process of Perthes disease can allow for the provision of earlier and more accurate diagnoses of the disease and more precise follow-ups and treatment in the early phases of the disease.
PB  - MDPI
T2  - Diagnostics
T1  - Molecular Biomarkers in Perthes Disease: A Review
IS  - 3
SP  - 471
VL  - 13
DO  - 10.3390/diagnostics13030471
ER  - 

@article{
author = "Spasovski, Vesna and Srzentić Dražilov, Sanja and Nikčević, Gordana and Baščarević, Zoran and Stojiljković, Maja and Pavlović, Sonja and Spasovski, Duško",
year = "2023",
abstract = "Background: Perthes disease is a juvenile form of osteonecrosis of the femoral head that affects children under the age of 15. One hundred years after its discovery, some light has been shed on its etiology and the biological factors relevant to its etiology and disease severity. Methods: The aim of this study was to summarize the literature findings on the biological factors relevant to the pathogenesis of Perthes disease, their diagnostic and clinical significance, and their therapeutic potential. A special focus on candidate genes as susceptibility factors and factors relevant to clinical severity was made, where studies reporting clinical or preclinical results were considered as the inclusion criteria. PubMed databases were searched by two independent researchers. Sixty-eight articles were included in this review. Results on the factors relevant to vascular involvement and inflammatory molecules indicated as factors that contribute to impaired bone remodeling have been summarized. Moreover, several candidate genes relevant to an active phase of the disease have been suggested as possible biological therapeutic targets. Conclusions: Delineation of molecular biomarkers that underlie the pathophysiological process of Perthes disease can allow for the provision of earlier and more accurate diagnoses of the disease and more precise follow-ups and treatment in the early phases of the disease.",
publisher = "MDPI",
journal = "Diagnostics",
title = "Molecular Biomarkers in Perthes Disease: A Review",
number = "3",
pages = "471",
volume = "13",
doi = "10.3390/diagnostics13030471"
}

Spasovski, V., Srzentić Dražilov, S., Nikčević, G., Baščarević, Z., Stojiljković, M., Pavlović, S.,& Spasovski, D.. (2023). Molecular Biomarkers in Perthes Disease: A Review. in Diagnostics
MDPI., 13(3), 471.
https://doi.org/10.3390/diagnostics13030471

Spasovski V, Srzentić Dražilov S, Nikčević G, Baščarević Z, Stojiljković M, Pavlović S, Spasovski D. Molecular Biomarkers in Perthes Disease: A Review. in Diagnostics. 2023;13(3):471.
doi:10.3390/diagnostics13030471 .

Spasovski, Vesna, Srzentić Dražilov, Sanja, Nikčević, Gordana, Baščarević, Zoran, Stojiljković, Maja, Pavlović, Sonja, Spasovski, Duško, "Molecular Biomarkers in Perthes Disease: A Review" in Diagnostics, 13, no. 3 (2023):471,
https://doi.org/10.3390/diagnostics13030471 . .

TY  - JOUR
AU  - Stanković, Biljana
AU  - Kotur, Nikola
AU  - Nikčević, Gordana
AU  - Gašić, Vladimir
AU  - Zukić, Branka
AU  - Pavlović, Sonja
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1460
AB  - Research of inflammatory bowel disease (IBD) has identified numerous molecular players involved in the disease development. Even so, the understanding of IBD is incomplete, while disease treatment is still far from the precision medicine. Reliable diagnostic and prognostic biomarkers in IBD are limited which may reduce efficient therapeutic outcomes. High-throughput technologies and artificial intelligence emerged as powerful tools in search of unrevealed molecular patterns that could give important insights into IBD pathogenesis and help to address unmet clinical needs. Machine learning, a subtype of artificial intelligence, uses complex mathematical algorithms to learn from existing data in order to predict future outcomes. The scientific community has been increasingly employing machine learning for the prediction of IBD outcomes from comprehensive patient data-clinical records, genomic, transcriptomic, proteomic, metagenomic, and other IBD relevant omics data. This review aims to present fundamental principles behind machine learning modeling and its current application in IBD research with the focus on studies that explored genomic and transcriptomic data. We described different strategies used for dealing with omics data and outlined the best-performing methods. Before being translated into clinical settings, the developed machine learning models should be tested in independent prospective studies as well as randomized controlled trials.
PB  - MDPI, Basel
T2  - Genes
T1  - Machine Learning Modeling from Omics Data as Prospective Tool for Improvement of Inflammatory Bowel Disease Diagnosis and Clinical Classifications
IS  - 9
VL  - 12
DO  - 10.3390/genes12091438
ER  - 

@article{
author = "Stanković, Biljana and Kotur, Nikola and Nikčević, Gordana and Gašić, Vladimir and Zukić, Branka and Pavlović, Sonja",
year = "2021",
abstract = "Research of inflammatory bowel disease (IBD) has identified numerous molecular players involved in the disease development. Even so, the understanding of IBD is incomplete, while disease treatment is still far from the precision medicine. Reliable diagnostic and prognostic biomarkers in IBD are limited which may reduce efficient therapeutic outcomes. High-throughput technologies and artificial intelligence emerged as powerful tools in search of unrevealed molecular patterns that could give important insights into IBD pathogenesis and help to address unmet clinical needs. Machine learning, a subtype of artificial intelligence, uses complex mathematical algorithms to learn from existing data in order to predict future outcomes. The scientific community has been increasingly employing machine learning for the prediction of IBD outcomes from comprehensive patient data-clinical records, genomic, transcriptomic, proteomic, metagenomic, and other IBD relevant omics data. This review aims to present fundamental principles behind machine learning modeling and its current application in IBD research with the focus on studies that explored genomic and transcriptomic data. We described different strategies used for dealing with omics data and outlined the best-performing methods. Before being translated into clinical settings, the developed machine learning models should be tested in independent prospective studies as well as randomized controlled trials.",
publisher = "MDPI, Basel",
journal = "Genes",
title = "Machine Learning Modeling from Omics Data as Prospective Tool for Improvement of Inflammatory Bowel Disease Diagnosis and Clinical Classifications",
number = "9",
volume = "12",
doi = "10.3390/genes12091438"
}

Stanković, B., Kotur, N., Nikčević, G., Gašić, V., Zukić, B.,& Pavlović, S.. (2021). Machine Learning Modeling from Omics Data as Prospective Tool for Improvement of Inflammatory Bowel Disease Diagnosis and Clinical Classifications. in Genes
MDPI, Basel., 12(9).
https://doi.org/10.3390/genes12091438

Stanković B, Kotur N, Nikčević G, Gašić V, Zukić B, Pavlović S. Machine Learning Modeling from Omics Data as Prospective Tool for Improvement of Inflammatory Bowel Disease Diagnosis and Clinical Classifications. in Genes. 2021;12(9).
doi:10.3390/genes12091438 .

Stanković, Biljana, Kotur, Nikola, Nikčević, Gordana, Gašić, Vladimir, Zukić, Branka, Pavlović, Sonja, "Machine Learning Modeling from Omics Data as Prospective Tool for Improvement of Inflammatory Bowel Disease Diagnosis and Clinical Classifications" in Genes, 12, no. 9 (2021),
https://doi.org/10.3390/genes12091438 . .

TY  - JOUR
AU  - Mrkovacki, Janko
AU  - Srzentić Dražilov, Sanja
AU  - Spasovski, Vesna
AU  - Fazlagić, Amira
AU  - Pavlović, Sonja
AU  - Nikčević, Gordana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1476
AB  - The management of degenerative lumbosacral stenosis (DLSS) in dogs usually requires aggressive, costly surgical treatments that may themselves present complications, while do not fully resolve the symptoms of the disease. In this study, the dog diagnosed with severe DLSS, with hind limb paresis, was treated using a new and least invasive treatment. Cultured autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) were injected bilaterally at the level of L7-S1, in the vicinity of the external aperture of the intervertebral foramen of DLSS patient. In the previously described treatments of spontaneous intervertebral disc degeneration in dogs, intradiscal injections of MSCs did not lead to positive effects. Here, we report a marked improvement in clinical outcome measures related to the ability of a dog to walk and trot, which were expressed by a numeric rating scale based on a veterinary assessment questionnaire. The improved status persisted throughout the observed time course of 4.5 years after the AT-MSC transplantation. To the best of our knowledge, this is the first case of successful therapy, with long-term positive effect, of spontaneously occurring canine DLSS using presented treatment that, we believe, represents a contribution to current knowledge in this field and may shape both animal and human DLSS treatment options.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Veterinary Science
T1  - Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells
VL  - 8
DO  - 10.3389/fvets.2021.732073
ER  - 

@article{
author = "Mrkovacki, Janko and Srzentić Dražilov, Sanja and Spasovski, Vesna and Fazlagić, Amira and Pavlović, Sonja and Nikčević, Gordana",
year = "2021",
abstract = "The management of degenerative lumbosacral stenosis (DLSS) in dogs usually requires aggressive, costly surgical treatments that may themselves present complications, while do not fully resolve the symptoms of the disease. In this study, the dog diagnosed with severe DLSS, with hind limb paresis, was treated using a new and least invasive treatment. Cultured autologous adipose tissue-derived mesenchymal stem cells (AT-MSCs) were injected bilaterally at the level of L7-S1, in the vicinity of the external aperture of the intervertebral foramen of DLSS patient. In the previously described treatments of spontaneous intervertebral disc degeneration in dogs, intradiscal injections of MSCs did not lead to positive effects. Here, we report a marked improvement in clinical outcome measures related to the ability of a dog to walk and trot, which were expressed by a numeric rating scale based on a veterinary assessment questionnaire. The improved status persisted throughout the observed time course of 4.5 years after the AT-MSC transplantation. To the best of our knowledge, this is the first case of successful therapy, with long-term positive effect, of spontaneously occurring canine DLSS using presented treatment that, we believe, represents a contribution to current knowledge in this field and may shape both animal and human DLSS treatment options.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Veterinary Science",
title = "Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells",
volume = "8",
doi = "10.3389/fvets.2021.732073"
}

Mrkovacki, J., Srzentić Dražilov, S., Spasovski, V., Fazlagić, A., Pavlović, S.,& Nikčević, G.. (2021). Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells. in Frontiers in Veterinary Science
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fvets.2021.732073

Mrkovacki J, Srzentić Dražilov S, Spasovski V, Fazlagić A, Pavlović S, Nikčević G. Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells. in Frontiers in Veterinary Science. 2021;8.
doi:10.3389/fvets.2021.732073 .

Mrkovacki, Janko, Srzentić Dražilov, Sanja, Spasovski, Vesna, Fazlagić, Amira, Pavlović, Sonja, Nikčević, Gordana, "Case Report: Successful Therapy of Spontaneously Occurring Canine Degenerative Lumbosacral Stenosis Using Autologous Adipose Tissue-Derived Mesenchymal Stem Cells" in Frontiers in Veterinary Science, 8 (2021),
https://doi.org/10.3389/fvets.2021.732073 . .

TY  - JOUR
AU  - Nikčević, Gordana
AU  - Srzentić Dražilov, Sanja
AU  - Karan-Đurašević, Teodora
AU  - Tošić, Nataša
AU  - Kontos, Christos K.
AU  - Scorilas, Andreas
AU  - Pavlović, Sonja
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1326
AB  - The BCL2L12, one of the latest discovered members of the BCL2 family, has both pro- and anti-apoptotic roles that are cell-type-dependent. Its role in tumorigenesis is highly implicated. Sixty-three splice variants of this gene have been identified so far, with significant differences in expression patterns between various cancer cell lines. Presently, little is known regarding the regulation of expression of the BCL2L12 gene. For the vast majority of BCL2L12 gene splice variants, the 5'- and 3'-untranslated regions as well as their transcriptional regulation have not been determined yet. The aim of this study was to get insight into the regulation of the BCL2L12 gene transcription in human chronic myelogenous leukemia (K562) cell line. Our results point to the activity of novel transcription start site of the BCL2L12 gene and indicate that Sp1 and GATA-1 transcription factors could be involved in the regulation of BCL2L12 gene expression in K562 cells. The previously reported active promoter of BCL2L12 gene differs from the one we described in our study. If this novel BCL2L12 promoter is confirmed to be active in other malignancies, transcripts generated from this region could be considered as new cancer-specific biomarkers. The results of our study contribute to the better understanding of the transcriptional regulation of the BCL2L12 gene.
PB  - Elsevier, Amsterdam
T2  - Gene
T1  - Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells
VL  - 750
DO  - 10.1016/j.gene.2020.144723
ER  - 

@article{
author = "Nikčević, Gordana and Srzentić Dražilov, Sanja and Karan-Đurašević, Teodora and Tošić, Nataša and Kontos, Christos K. and Scorilas, Andreas and Pavlović, Sonja",
year = "2020",
abstract = "The BCL2L12, one of the latest discovered members of the BCL2 family, has both pro- and anti-apoptotic roles that are cell-type-dependent. Its role in tumorigenesis is highly implicated. Sixty-three splice variants of this gene have been identified so far, with significant differences in expression patterns between various cancer cell lines. Presently, little is known regarding the regulation of expression of the BCL2L12 gene. For the vast majority of BCL2L12 gene splice variants, the 5'- and 3'-untranslated regions as well as their transcriptional regulation have not been determined yet. The aim of this study was to get insight into the regulation of the BCL2L12 gene transcription in human chronic myelogenous leukemia (K562) cell line. Our results point to the activity of novel transcription start site of the BCL2L12 gene and indicate that Sp1 and GATA-1 transcription factors could be involved in the regulation of BCL2L12 gene expression in K562 cells. The previously reported active promoter of BCL2L12 gene differs from the one we described in our study. If this novel BCL2L12 promoter is confirmed to be active in other malignancies, transcripts generated from this region could be considered as new cancer-specific biomarkers. The results of our study contribute to the better understanding of the transcriptional regulation of the BCL2L12 gene.",
publisher = "Elsevier, Amsterdam",
journal = "Gene",
title = "Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells",
volume = "750",
doi = "10.1016/j.gene.2020.144723"
}

Nikčević, G., Srzentić Dražilov, S., Karan-Đurašević, T., Tošić, N., Kontos, C. K., Scorilas, A.,& Pavlović, S.. (2020). Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells. in Gene
Elsevier, Amsterdam., 750.
https://doi.org/10.1016/j.gene.2020.144723

Nikčević G, Srzentić Dražilov S, Karan-Đurašević T, Tošić N, Kontos CK, Scorilas A, Pavlović S. Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells. in Gene. 2020;750.
doi:10.1016/j.gene.2020.144723 .

Nikčević, Gordana, Srzentić Dražilov, Sanja, Karan-Đurašević, Teodora, Tošić, Nataša, Kontos, Christos K., Scorilas, Andreas, Pavlović, Sonja, "Complex transcriptional regulation of the BCL2L12 gene: Novel, active promoter in K562 cells" in Gene, 750 (2020),
https://doi.org/10.1016/j.gene.2020.144723 . .

TY  - JOUR
AU  - Stanković, Biljana
AU  - Dragasević, Sanja
AU  - Klaassen, Kristel
AU  - Kotur, Nikola
AU  - Srzentić Dražilov, Sanja
AU  - Zukić, Branka
AU  - Sokic Milutinović, Aleksandra
AU  - Milovanović, Tamara
AU  - Lukić, Snežana
AU  - Popović, Dragan
AU  - Pavlović, Sonja
AU  - Nikčević, Gordana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1329
AB  - Background: Crohn's disease (CD) is chronic inflammatory bowel disease with different phenotypic characteristics influencing disease prognosis and therapeutic strategies. The aim of this pilot study was to analyze selected inflammatory and apoptotic markers in non-inflamed and inflamed samples of ileal mucosa of non-stricturing/non-penetrating (NS/NP) and stricturing (S) CD mucosal phenotypes in order to characterize their distinct profiles. Methods: From twenty CD patients (9 NS/NP, 11 S) paired non-inflamed and inflamed ileal biopsies were collected and used for analysis of cytokine (TNF and IL6) and apoptotic (Bcl2, Box, Fas and FasL) genes' expression levels by real-time PCR, while NF kappa B transcriptional potency was assessed by electromobility gel shift assay. Results: Our results demonstrated significant upregulation of TNF and IL6 in inflamed area of both NS/NP (p = 0.03, p = 0.01) and S phenotypes (p = 0.04, p = 0.04), respectively. However, TNF increase was more prominent in NS/NP compared to S inflamed mucosa (p = 0.02). Also, level of proapoptotic Box was significantly higher in NS/NP compared to S inflamed mucosa (p = 0.01). Opposing transcription potency of NF kappa B has been detected between two phenotypes: being decreased in NS/NP (p = 0.07) and increased in S (p = 0.1) inflamed compared to non-inflamed mucosa, demonstrating trend towards statistical significance. Conclusions: We found that two distinct CD phenotypes have specific molecular signatures. Obtained results could direct improvement of current and development of new therapeutic strategies based on more specific molecular stratification of CD patients.
PB  - Elsevier Gmbh, Munich
T2  - Pathology Research and Practice
T1  - Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy
IS  - 6
VL  - 216
DO  - 10.1016/j.prp.2020.152945
ER  - 

@article{
author = "Stanković, Biljana and Dragasević, Sanja and Klaassen, Kristel and Kotur, Nikola and Srzentić Dražilov, Sanja and Zukić, Branka and Sokic Milutinović, Aleksandra and Milovanović, Tamara and Lukić, Snežana and Popović, Dragan and Pavlović, Sonja and Nikčević, Gordana",
year = "2020",
abstract = "Background: Crohn's disease (CD) is chronic inflammatory bowel disease with different phenotypic characteristics influencing disease prognosis and therapeutic strategies. The aim of this pilot study was to analyze selected inflammatory and apoptotic markers in non-inflamed and inflamed samples of ileal mucosa of non-stricturing/non-penetrating (NS/NP) and stricturing (S) CD mucosal phenotypes in order to characterize their distinct profiles. Methods: From twenty CD patients (9 NS/NP, 11 S) paired non-inflamed and inflamed ileal biopsies were collected and used for analysis of cytokine (TNF and IL6) and apoptotic (Bcl2, Box, Fas and FasL) genes' expression levels by real-time PCR, while NF kappa B transcriptional potency was assessed by electromobility gel shift assay. Results: Our results demonstrated significant upregulation of TNF and IL6 in inflamed area of both NS/NP (p = 0.03, p = 0.01) and S phenotypes (p = 0.04, p = 0.04), respectively. However, TNF increase was more prominent in NS/NP compared to S inflamed mucosa (p = 0.02). Also, level of proapoptotic Box was significantly higher in NS/NP compared to S inflamed mucosa (p = 0.01). Opposing transcription potency of NF kappa B has been detected between two phenotypes: being decreased in NS/NP (p = 0.07) and increased in S (p = 0.1) inflamed compared to non-inflamed mucosa, demonstrating trend towards statistical significance. Conclusions: We found that two distinct CD phenotypes have specific molecular signatures. Obtained results could direct improvement of current and development of new therapeutic strategies based on more specific molecular stratification of CD patients.",
publisher = "Elsevier Gmbh, Munich",
journal = "Pathology Research and Practice",
title = "Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy",
number = "6",
volume = "216",
doi = "10.1016/j.prp.2020.152945"
}

Stanković, B., Dragasević, S., Klaassen, K., Kotur, N., Srzentić Dražilov, S., Zukić, B., Sokic Milutinović, A., Milovanović, T., Lukić, S., Popović, D., Pavlović, S.,& Nikčević, G.. (2020). Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy. in Pathology Research and Practice
Elsevier Gmbh, Munich., 216(6).
https://doi.org/10.1016/j.prp.2020.152945

Stanković B, Dragasević S, Klaassen K, Kotur N, Srzentić Dražilov S, Zukić B, Sokic Milutinović A, Milovanović T, Lukić S, Popović D, Pavlović S, Nikčević G. Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy. in Pathology Research and Practice. 2020;216(6).
doi:10.1016/j.prp.2020.152945 .

Stanković, Biljana, Dragasević, Sanja, Klaassen, Kristel, Kotur, Nikola, Srzentić Dražilov, Sanja, Zukić, Branka, Sokic Milutinović, Aleksandra, Milovanović, Tamara, Lukić, Snežana, Popović, Dragan, Pavlović, Sonja, Nikčević, Gordana, "Exploring inflammatory and apoptotic signatures in distinct Crohn's disease phenotypes: Way towards molecular stratification of patients and targeted therapy" in Pathology Research and Practice, 216, no. 6 (2020),
https://doi.org/10.1016/j.prp.2020.152945 . .

TY  - JOUR
AU  - Srzentić Dražilov, Sanja
AU  - Mrkovacki, Janko
AU  - Spasovski, Vesna
AU  - Fazlagić, Amira
AU  - Pavlović, Sonja
AU  - Nikčević, Gordana
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1142
AB  - Mesenchymal stem cells (MSCs) hold enormous potential for cell-based therapy in the treatment of various diseases, particularly those which currently cannot be cured and result in poor outcomes or invasive surgery. Here we present results of the application of autologous, culture-expanded, adipose tissue (AT)derived MSCs for the osteoarthritis (OA) treatment of 10 canine patients. The stemness of isolated cells has been confirmed by their ability to differentiate into osteo-and chondrocytic lineages. The clinical effect of a single injection of AT-MSCs into the symptomatic joints was evaluated by a veterinarian for five disabilities characteristic of OA at 30, 60 and 90 days after treatment, which has been designated as the initial evaluation period. Functional outcomes for all analysed characteristics improved significantly at the end of this evaluation compared with the baseline. In addition, for 5 of these 10 patients, an extended follow-up study was performed from 1 to 4 years after the initial evaluation period. We detected long-lasting positive effects on two out of five analysed characteristics. The results demonstrate that the use of autologous AT-MSCs is a successful approach to canine OA therapy.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - The use of canine mesenchymal stem cells for the autologous treatment of osteoarthritis
EP  - 389
EP  - 
IS  - 3
SP  - 376
VL  - 66
DO  - 10.1556/004.2018.034
ER  - 

@article{
author = "Srzentić Dražilov, Sanja and Mrkovacki, Janko and Spasovski, Vesna and Fazlagić, Amira and Pavlović, Sonja and Nikčević, Gordana",
year = "2018",
abstract = "Mesenchymal stem cells (MSCs) hold enormous potential for cell-based therapy in the treatment of various diseases, particularly those which currently cannot be cured and result in poor outcomes or invasive surgery. Here we present results of the application of autologous, culture-expanded, adipose tissue (AT)derived MSCs for the osteoarthritis (OA) treatment of 10 canine patients. The stemness of isolated cells has been confirmed by their ability to differentiate into osteo-and chondrocytic lineages. The clinical effect of a single injection of AT-MSCs into the symptomatic joints was evaluated by a veterinarian for five disabilities characteristic of OA at 30, 60 and 90 days after treatment, which has been designated as the initial evaluation period. Functional outcomes for all analysed characteristics improved significantly at the end of this evaluation compared with the baseline. In addition, for 5 of these 10 patients, an extended follow-up study was performed from 1 to 4 years after the initial evaluation period. We detected long-lasting positive effects on two out of five analysed characteristics. The results demonstrate that the use of autologous AT-MSCs is a successful approach to canine OA therapy.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "The use of canine mesenchymal stem cells for the autologous treatment of osteoarthritis",
pages = "389--376",
number = "3",
volume = "66",
doi = "10.1556/004.2018.034"
}

Srzentić Dražilov, S., Mrkovacki, J., Spasovski, V., Fazlagić, A., Pavlović, S.,& Nikčević, G.. (2018). The use of canine mesenchymal stem cells for the autologous treatment of osteoarthritis. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 66(3), 376-389.
https://doi.org/10.1556/004.2018.034

Srzentić Dražilov S, Mrkovacki J, Spasovski V, Fazlagić A, Pavlović S, Nikčević G. The use of canine mesenchymal stem cells for the autologous treatment of osteoarthritis. in Acta Veterinaria Hungarica. 2018;66(3):376-389.
doi:10.1556/004.2018.034 .

Srzentić Dražilov, Sanja, Mrkovacki, Janko, Spasovski, Vesna, Fazlagić, Amira, Pavlović, Sonja, Nikčević, Gordana, "The use of canine mesenchymal stem cells for the autologous treatment of osteoarthritis" in Acta Veterinaria Hungarica, 66, no. 3 (2018):376-389,
https://doi.org/10.1556/004.2018.034 . .

TY  - JOUR
AU  - Klaassen, Kristel
AU  - Stanković, Biljana
AU  - Kotur, Nikola
AU  - Đorđević, Maja
AU  - Zukić, Branka
AU  - Nikčević, Gordana
AU  - Ugrin, Milena
AU  - Spasovski, Vesna
AU  - Srzentić Dražilov, Sanja
AU  - Pavlović, Sonja
AU  - Stojiljković, Maja
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1060
AB  - Phenylketonuria (PKU) is a metabolic disease caused by mutations in the phenylalanine hydroxylase (PAH) gene. Although the PAH genotype remains the main determinant of PKU phenotype severity, genotype-phenotype inconsistencies have been reported. In this study, we focused on unanalysed sequences in non-coding PAH gene regions to assess their possible influence on the PKU phenotype. We transiently transfected HepG2 cells with various chloramphenicol acetyl transferase (CAT) reporter constructs which included PAH gene non-coding regions. Selected non-coding regions were indicated by in silico prediction to contain transcription factor binding sites. Furthermore, electrophoretic mobility shift assay (EMSA) and supershift assays were performed to identify which transcriptional factors were engaged in the interaction. We found novel KLF1 motif in the PAH promoter, which decreases CAT activity by 50 % in comparison to basal transcription in vitro. The cytosine at the c.-170 promoter position creates an additional binding site for the protein complex involving KLF1 transcription factor. Moreover, we assessed for the first time the role of a multivariant variable number tandem repeat (VNTR) region located in the 3'-region of the PAH gene. We found that the VNTR3, VNTR7 and VNTR8 constructs had approximately 60 % of CAT activity. The regulation is mediated by the C/EBPalpha transcription factor, present in protein complex binding to VNTR3. Our study highlighted two novel promoter KLF1 and 3'-region C/EBPalpha motifs in the PAH gene which decrease transcription in vitro and, thus, could be considered as PAH expression modifiers. New transcription motifs in non-coding regions will contribute to better understanding of the PKU phenotype complexity and may become important for the optimisation of PKU treatment.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Applied Genetics
T1  - New PAH gene promoter KLF1 and 3'-region C/EBPalpha motifs influence transcription in vitro
EP  - 85
IS  - 1
SP  - 79
VL  - 58
DO  - 10.1007/s13353-016-0359-0
ER  - 

@article{
author = "Klaassen, Kristel and Stanković, Biljana and Kotur, Nikola and Đorđević, Maja and Zukić, Branka and Nikčević, Gordana and Ugrin, Milena and Spasovski, Vesna and Srzentić Dražilov, Sanja and Pavlović, Sonja and Stojiljković, Maja",
year = "2017",
abstract = "Phenylketonuria (PKU) is a metabolic disease caused by mutations in the phenylalanine hydroxylase (PAH) gene. Although the PAH genotype remains the main determinant of PKU phenotype severity, genotype-phenotype inconsistencies have been reported. In this study, we focused on unanalysed sequences in non-coding PAH gene regions to assess their possible influence on the PKU phenotype. We transiently transfected HepG2 cells with various chloramphenicol acetyl transferase (CAT) reporter constructs which included PAH gene non-coding regions. Selected non-coding regions were indicated by in silico prediction to contain transcription factor binding sites. Furthermore, electrophoretic mobility shift assay (EMSA) and supershift assays were performed to identify which transcriptional factors were engaged in the interaction. We found novel KLF1 motif in the PAH promoter, which decreases CAT activity by 50 % in comparison to basal transcription in vitro. The cytosine at the c.-170 promoter position creates an additional binding site for the protein complex involving KLF1 transcription factor. Moreover, we assessed for the first time the role of a multivariant variable number tandem repeat (VNTR) region located in the 3'-region of the PAH gene. We found that the VNTR3, VNTR7 and VNTR8 constructs had approximately 60 % of CAT activity. The regulation is mediated by the C/EBPalpha transcription factor, present in protein complex binding to VNTR3. Our study highlighted two novel promoter KLF1 and 3'-region C/EBPalpha motifs in the PAH gene which decrease transcription in vitro and, thus, could be considered as PAH expression modifiers. New transcription motifs in non-coding regions will contribute to better understanding of the PKU phenotype complexity and may become important for the optimisation of PKU treatment.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Applied Genetics",
title = "New PAH gene promoter KLF1 and 3'-region C/EBPalpha motifs influence transcription in vitro",
pages = "85-79",
number = "1",
volume = "58",
doi = "10.1007/s13353-016-0359-0"
}

Klaassen, K., Stanković, B., Kotur, N., Đorđević, M., Zukić, B., Nikčević, G., Ugrin, M., Spasovski, V., Srzentić Dražilov, S., Pavlović, S.,& Stojiljković, M.. (2017). New PAH gene promoter KLF1 and 3'-region C/EBPalpha motifs influence transcription in vitro. in Journal of Applied Genetics
Springer Heidelberg, Heidelberg., 58(1), 79-85.
https://doi.org/10.1007/s13353-016-0359-0

Klaassen K, Stanković B, Kotur N, Đorđević M, Zukić B, Nikčević G, Ugrin M, Spasovski V, Srzentić Dražilov S, Pavlović S, Stojiljković M. New PAH gene promoter KLF1 and 3'-region C/EBPalpha motifs influence transcription in vitro. in Journal of Applied Genetics. 2017;58(1):79-85.
doi:10.1007/s13353-016-0359-0 .

Klaassen, Kristel, Stanković, Biljana, Kotur, Nikola, Đorđević, Maja, Zukić, Branka, Nikčević, Gordana, Ugrin, Milena, Spasovski, Vesna, Srzentić Dražilov, Sanja, Pavlović, Sonja, Stojiljković, Maja, "New PAH gene promoter KLF1 and 3'-region C/EBPalpha motifs influence transcription in vitro" in Journal of Applied Genetics, 58, no. 1 (2017):79-85,
https://doi.org/10.1007/s13353-016-0359-0 . .

TY  - JOUR
AU  - Dragasević, Sanja
AU  - Stanković, Biljana
AU  - Milosavljević, Tomica
AU  - Sokić-Milutinović, Aleksandra
AU  - Lukić, Snežana
AU  - Alempijević, Tamara
AU  - Zukić, Branka
AU  - Kotur, Nikola
AU  - Nikčević, Gordana
AU  - Pavlović, Sonja
AU  - Popović, Dragan
PY  - 2017
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1063
AB  - Objectives The aim of the study was to evaluate associations between inflammatory bowel disease (IBD) presentation and variants in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta, and IL-RN genes in order to identify possible environmental factors that may affect IBD occurrence, investigate potential predictors for surgical treatment of IBD, and correlate the presence of granulomas in biopsy specimens with clinical characteristics of Crohn's disease (CD) patients. Patients and methods We genotyped 167 IBD patients using PCR-based methodology and tested for disease genotype-phenotype associations. Results In CD patients ileal localization of disease was more frequent in NOD2 variant carriers. Ileal CD was associated with IL-6 GC+CC genotypes, identifying C allele as a possible marker of increased risk for ileal CD. In CD patients a positive family history for IBD was related to earlier onset of disease, higher risk for CD-related surgery, and appendectomy. CD patients who are TLR4 299Gly carriers are at higher risk for surgery at onset of the disease compared with TLR4 299Asp variant carriers. The presence of granuloma in biopsy specimens was more frequent in patients in whom a diagnosis of CD was made during emergency surgery. Multivariate analysis showed that CD carriers of the 299Gly allele had a 4.6-fold higher risk for emergency surgery before CD diagnosis is established compared with noncarriers, suggesting an aggressive disease course. Granuloma in endoscopic biopsies is detected 5.4-fold more frequently in patients treated surgically at the time of diagnosis. Conclusion Genetic variants together with epidemiological and clinical data of IBD patients could potentially be used as predictors of the disease course.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - European Journal of Gastroenterology & Hepatology
T1  - Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease
EP  - 915
IS  - 8
SP  - 909
VL  - 29
DO  - 10.1097/MEG.0000000000000877
ER  - 

@article{
author = "Dragasević, Sanja and Stanković, Biljana and Milosavljević, Tomica and Sokić-Milutinović, Aleksandra and Lukić, Snežana and Alempijević, Tamara and Zukić, Branka and Kotur, Nikola and Nikčević, Gordana and Pavlović, Sonja and Popović, Dragan",
year = "2017",
abstract = "Objectives The aim of the study was to evaluate associations between inflammatory bowel disease (IBD) presentation and variants in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta, and IL-RN genes in order to identify possible environmental factors that may affect IBD occurrence, investigate potential predictors for surgical treatment of IBD, and correlate the presence of granulomas in biopsy specimens with clinical characteristics of Crohn's disease (CD) patients. Patients and methods We genotyped 167 IBD patients using PCR-based methodology and tested for disease genotype-phenotype associations. Results In CD patients ileal localization of disease was more frequent in NOD2 variant carriers. Ileal CD was associated with IL-6 GC+CC genotypes, identifying C allele as a possible marker of increased risk for ileal CD. In CD patients a positive family history for IBD was related to earlier onset of disease, higher risk for CD-related surgery, and appendectomy. CD patients who are TLR4 299Gly carriers are at higher risk for surgery at onset of the disease compared with TLR4 299Asp variant carriers. The presence of granuloma in biopsy specimens was more frequent in patients in whom a diagnosis of CD was made during emergency surgery. Multivariate analysis showed that CD carriers of the 299Gly allele had a 4.6-fold higher risk for emergency surgery before CD diagnosis is established compared with noncarriers, suggesting an aggressive disease course. Granuloma in endoscopic biopsies is detected 5.4-fold more frequently in patients treated surgically at the time of diagnosis. Conclusion Genetic variants together with epidemiological and clinical data of IBD patients could potentially be used as predictors of the disease course.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "European Journal of Gastroenterology & Hepatology",
title = "Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease",
pages = "915-909",
number = "8",
volume = "29",
doi = "10.1097/MEG.0000000000000877"
}

Dragasević, S., Stanković, B., Milosavljević, T., Sokić-Milutinović, A., Lukić, S., Alempijević, T., Zukić, B., Kotur, N., Nikčević, G., Pavlović, S.,& Popović, D.. (2017). Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease. in European Journal of Gastroenterology & Hepatology
Lippincott Williams & Wilkins, Philadelphia., 29(8), 909-915.
https://doi.org/10.1097/MEG.0000000000000877

Dragasević S, Stanković B, Milosavljević T, Sokić-Milutinović A, Lukić S, Alempijević T, Zukić B, Kotur N, Nikčević G, Pavlović S, Popović D. Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease. in European Journal of Gastroenterology & Hepatology. 2017;29(8):909-915.
doi:10.1097/MEG.0000000000000877 .

Dragasević, Sanja, Stanković, Biljana, Milosavljević, Tomica, Sokić-Milutinović, Aleksandra, Lukić, Snežana, Alempijević, Tamara, Zukić, Branka, Kotur, Nikola, Nikčević, Gordana, Pavlović, Sonja, Popović, Dragan, "Genetic and environmental factors significant for the presentation and development of inflammatory bowel disease" in European Journal of Gastroenterology & Hepatology, 29, no. 8 (2017):909-915,
https://doi.org/10.1097/MEG.0000000000000877 . .

TY  - JOUR
AU  - Gazouli, Maria
AU  - Wouters, Mira M.
AU  - Kapur-Pojskić, Lejla
AU  - Bengtson, May-Bente
AU  - Friedman, Eitan
AU  - Nikčević, Gordana
AU  - Demetriou, Christiana A.
AU  - Mulak, Agata
AU  - Santos, Javier
AU  - Niesler, Beate
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/977
AB  - IBS is the most prevalent functional gastrointestinal disorder and phenotypically characterized by chronic abdominal discomfort, pain and altered defecation patterns. The pathophysiology of IBS is multifactorial, albeit with a substantial genetic component. To date, studies using various methodologies, ranging from family and twin studies to candidate gene approaches and genome-wide association studies, have identified several genetic variants in the context of IBS. Yet, despite enlarged sample sizes, increased statistical power and meta-analyses in the past 7 years, positive associations are still scarce and/or have not been reproduced. In addition, epigenetic and pharmacogenetic approaches remain in their infancy. A major hurdle is the lack of large homogenized case-control cohorts recruited according to standardized and harmonized criteria. The COST Action BM1106 GENIEUR (GENes in Irritable Bowel Syndrome Research Network EURope) has been established to address these obstacles. In this Review, the (epi)genetic working group of GENIEUR reports on the current state-of-the-art in the field, highlights fundamental flaws and pitfalls in current IBS (epi) genetic research and provides a vision on how to address and improve (epi) genetic approaches in this complex disorder in the future.
PB  - Nature Portfolio, Berlin
T2  - Nature Reviews Gastroenterology & Hepatology
T1  - Lessons learned - resolving the enigma of genetic factors in IBS
EP  - 87
IS  - 2
SP  - 77
VL  - 13
DO  - 10.1038/nrgastro.2015.206
ER  - 

@article{
author = "Gazouli, Maria and Wouters, Mira M. and Kapur-Pojskić, Lejla and Bengtson, May-Bente and Friedman, Eitan and Nikčević, Gordana and Demetriou, Christiana A. and Mulak, Agata and Santos, Javier and Niesler, Beate",
year = "2016",
abstract = "IBS is the most prevalent functional gastrointestinal disorder and phenotypically characterized by chronic abdominal discomfort, pain and altered defecation patterns. The pathophysiology of IBS is multifactorial, albeit with a substantial genetic component. To date, studies using various methodologies, ranging from family and twin studies to candidate gene approaches and genome-wide association studies, have identified several genetic variants in the context of IBS. Yet, despite enlarged sample sizes, increased statistical power and meta-analyses in the past 7 years, positive associations are still scarce and/or have not been reproduced. In addition, epigenetic and pharmacogenetic approaches remain in their infancy. A major hurdle is the lack of large homogenized case-control cohorts recruited according to standardized and harmonized criteria. The COST Action BM1106 GENIEUR (GENes in Irritable Bowel Syndrome Research Network EURope) has been established to address these obstacles. In this Review, the (epi)genetic working group of GENIEUR reports on the current state-of-the-art in the field, highlights fundamental flaws and pitfalls in current IBS (epi) genetic research and provides a vision on how to address and improve (epi) genetic approaches in this complex disorder in the future.",
publisher = "Nature Portfolio, Berlin",
journal = "Nature Reviews Gastroenterology & Hepatology",
title = "Lessons learned - resolving the enigma of genetic factors in IBS",
pages = "87-77",
number = "2",
volume = "13",
doi = "10.1038/nrgastro.2015.206"
}

Gazouli, M., Wouters, M. M., Kapur-Pojskić, L., Bengtson, M., Friedman, E., Nikčević, G., Demetriou, C. A., Mulak, A., Santos, J.,& Niesler, B.. (2016). Lessons learned - resolving the enigma of genetic factors in IBS. in Nature Reviews Gastroenterology & Hepatology
Nature Portfolio, Berlin., 13(2), 77-87.
https://doi.org/10.1038/nrgastro.2015.206

Gazouli M, Wouters MM, Kapur-Pojskić L, Bengtson M, Friedman E, Nikčević G, Demetriou CA, Mulak A, Santos J, Niesler B. Lessons learned - resolving the enigma of genetic factors in IBS. in Nature Reviews Gastroenterology & Hepatology. 2016;13(2):77-87.
doi:10.1038/nrgastro.2015.206 .

Gazouli, Maria, Wouters, Mira M., Kapur-Pojskić, Lejla, Bengtson, May-Bente, Friedman, Eitan, Nikčević, Gordana, Demetriou, Christiana A., Mulak, Agata, Santos, Javier, Niesler, Beate, "Lessons learned - resolving the enigma of genetic factors in IBS" in Nature Reviews Gastroenterology & Hepatology, 13, no. 2 (2016):77-87,
https://doi.org/10.1038/nrgastro.2015.206 . .

TY  - JOUR
AU  - Gazouli, Maria
AU  - Wouters, Mira M.
AU  - Kapur-Pojskić, Lejla
AU  - Bengtson, May-Bente
AU  - Friedman, Eitan
AU  - Nikčević, Gordana
AU  - Demetriou, Christiana A.
AU  - Mulak, Agata
AU  - Santos, Javier
AU  - Niesler, Beate
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1625
AB  - IBS is the most prevalent functional gastrointestinal disorder and phenotypically characterized by chronic abdominal discomfort, pain and altered defecation patterns. The pathophysiology of IBS is multifactorial, albeit with a substantial genetic component. To date, studies using various methodologies, ranging from family and twin studies to candidate gene approaches and genome-wide association studies, have identified several genetic variants in the context of IBS. Yet, despite enlarged sample sizes, increased statistical power and meta-analyses in the past 7 years, positive associations are still scarce and/or have not been reproduced. In addition, epigenetic and pharmacogenetic approaches remain in their infancy. A major hurdle is the lack of large homogenized case-control cohorts recruited according to standardized and harmonized criteria. The COST Action BM1106 GENIEUR (GENes in Irritable Bowel Syndrome Research Network EURope) has been established to address these obstacles. In this Review, the (epi)genetic working group of GENIEUR reports on the current state-of-the-art in the field, highlights fundamental flaws and pitfalls in current IBS (epi) genetic research and provides a vision on how to address and improve (epi) genetic approaches in this complex disorder in the future.
PB  - Nature Portfolio, Berlin
T2  - Nature Reviews Gastroenterology & Hepatology
T1  - Lessons learned - resolving the enigma of genetic factors in IBS
EP  - 87
IS  - 2
SP  - 77
VL  - 13
DO  - 10.1038/nrgastro.2015.206
ER  - 

@article{
author = "Gazouli, Maria and Wouters, Mira M. and Kapur-Pojskić, Lejla and Bengtson, May-Bente and Friedman, Eitan and Nikčević, Gordana and Demetriou, Christiana A. and Mulak, Agata and Santos, Javier and Niesler, Beate",
year = "2016",
abstract = "IBS is the most prevalent functional gastrointestinal disorder and phenotypically characterized by chronic abdominal discomfort, pain and altered defecation patterns. The pathophysiology of IBS is multifactorial, albeit with a substantial genetic component. To date, studies using various methodologies, ranging from family and twin studies to candidate gene approaches and genome-wide association studies, have identified several genetic variants in the context of IBS. Yet, despite enlarged sample sizes, increased statistical power and meta-analyses in the past 7 years, positive associations are still scarce and/or have not been reproduced. In addition, epigenetic and pharmacogenetic approaches remain in their infancy. A major hurdle is the lack of large homogenized case-control cohorts recruited according to standardized and harmonized criteria. The COST Action BM1106 GENIEUR (GENes in Irritable Bowel Syndrome Research Network EURope) has been established to address these obstacles. In this Review, the (epi)genetic working group of GENIEUR reports on the current state-of-the-art in the field, highlights fundamental flaws and pitfalls in current IBS (epi) genetic research and provides a vision on how to address and improve (epi) genetic approaches in this complex disorder in the future.",
publisher = "Nature Portfolio, Berlin",
journal = "Nature Reviews Gastroenterology & Hepatology",
title = "Lessons learned - resolving the enigma of genetic factors in IBS",
pages = "87-77",
number = "2",
volume = "13",
doi = "10.1038/nrgastro.2015.206"
}

Gazouli, M., Wouters, M. M., Kapur-Pojskić, L., Bengtson, M., Friedman, E., Nikčević, G., Demetriou, C. A., Mulak, A., Santos, J.,& Niesler, B.. (2016). Lessons learned - resolving the enigma of genetic factors in IBS. in Nature Reviews Gastroenterology & Hepatology
Nature Portfolio, Berlin., 13(2), 77-87.
https://doi.org/10.1038/nrgastro.2015.206

Gazouli M, Wouters MM, Kapur-Pojskić L, Bengtson M, Friedman E, Nikčević G, Demetriou CA, Mulak A, Santos J, Niesler B. Lessons learned - resolving the enigma of genetic factors in IBS. in Nature Reviews Gastroenterology & Hepatology. 2016;13(2):77-87.
doi:10.1038/nrgastro.2015.206 .

Gazouli, Maria, Wouters, Mira M., Kapur-Pojskić, Lejla, Bengtson, May-Bente, Friedman, Eitan, Nikčević, Gordana, Demetriou, Christiana A., Mulak, Agata, Santos, Javier, Niesler, Beate, "Lessons learned - resolving the enigma of genetic factors in IBS" in Nature Reviews Gastroenterology & Hepatology, 13, no. 2 (2016):77-87,
https://doi.org/10.1038/nrgastro.2015.206 . .

TY  - JOUR
AU  - Balasopoulou, Angeliki
AU  - Stanković, Biljana
AU  - Panagiotara, Angeliki
AU  - Nikčević, Gordana
AU  - Peters, Brock A.
AU  - John, Anne
AU  - Mendrinou, Effrosyni
AU  - Stratopoulos, Apostolos
AU  - Legaki, Aigli Ioanna
AU  - Stathakopoulou, Vasiliki
AU  - Tsolia, Aristoniki
AU  - Govaris, Nikolaos
AU  - Govari, Sofia
AU  - Zagoriti, Zoi
AU  - Poulas, Konstantinos
AU  - Kanariou, Maria
AU  - Constantinidou, Nikki
AU  - Krini, Maro
AU  - Spanou, Kleopatra
AU  - Radlović, Nedeljko
AU  - Ali, Bassam R.
AU  - Borg, Joseph
AU  - Drmanac, Radoje
AU  - Chrousos, George
AU  - Pavlović, Sonja
AU  - Roma, Eleftheria
AU  - Zukić, Branka
AU  - Patrinos, George P.
AU  - Katsila, Theodora
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/918
AB  - Background: Celiac disease is a complex chronic immune-mediated disorder of the small intestine. Today, the pathobiology of the disease is unclear, perplexing differential diagnosis, patient stratification, and decision-making in the clinic. Methods: Herein, we adopted a next-generation sequencing approach in a celiac disease trio of Greek descent to identify all genomic variants with the potential of celiac disease predisposition. Results: Analysis revealed six genomic variants of prime interest: SLC9A4 c.1919G gt A, KIAA1109 c.2933T gt C and c. 4268_4269delCCinsTA, HoxB6 c.668C gt A, HoxD12 c.418G gt A, and NCK2 c.745_746delAAinsG, from which NCK2 c.745_746delAAinsG is novel. Data validation in pediatric celiac disease patients of Greek (n=109) and Serbian (n=73) descent and their healthy counterparts (n=111 and n=32, respectively) indicated that HoxD12 c.418G gt A is more prevalent in celiac disease patients in the Serbian population (P  lt  0.01), while NCK2 c.745_746delAAinsG is less prevalent in celiac disease patients rather than healthy individuals of Greek descent (P = 0. 03). SLC9A4 c.1919G gt A and KIAA1109 c.2933T gt C and c.4268_4269delCCinsTA were more abundant in patients; nevertheless, they failed to show statistical significance. Conclusions: The next-generation sequencing-based family genomics approach described herein may serve as a paradigm towards the identification of novel functional variants with the aim of understanding complex disease pathobiology.
PB  - Biomed Central Ltd, London
T2  - Human Genomics
T1  - Novel genetic risk variants for pediatric celiac disease
VL  - 10
DO  - 10.1186/s40246-016-0091-1
ER  - 

@article{
author = "Balasopoulou, Angeliki and Stanković, Biljana and Panagiotara, Angeliki and Nikčević, Gordana and Peters, Brock A. and John, Anne and Mendrinou, Effrosyni and Stratopoulos, Apostolos and Legaki, Aigli Ioanna and Stathakopoulou, Vasiliki and Tsolia, Aristoniki and Govaris, Nikolaos and Govari, Sofia and Zagoriti, Zoi and Poulas, Konstantinos and Kanariou, Maria and Constantinidou, Nikki and Krini, Maro and Spanou, Kleopatra and Radlović, Nedeljko and Ali, Bassam R. and Borg, Joseph and Drmanac, Radoje and Chrousos, George and Pavlović, Sonja and Roma, Eleftheria and Zukić, Branka and Patrinos, George P. and Katsila, Theodora",
year = "2016",
abstract = "Background: Celiac disease is a complex chronic immune-mediated disorder of the small intestine. Today, the pathobiology of the disease is unclear, perplexing differential diagnosis, patient stratification, and decision-making in the clinic. Methods: Herein, we adopted a next-generation sequencing approach in a celiac disease trio of Greek descent to identify all genomic variants with the potential of celiac disease predisposition. Results: Analysis revealed six genomic variants of prime interest: SLC9A4 c.1919G gt A, KIAA1109 c.2933T gt C and c. 4268_4269delCCinsTA, HoxB6 c.668C gt A, HoxD12 c.418G gt A, and NCK2 c.745_746delAAinsG, from which NCK2 c.745_746delAAinsG is novel. Data validation in pediatric celiac disease patients of Greek (n=109) and Serbian (n=73) descent and their healthy counterparts (n=111 and n=32, respectively) indicated that HoxD12 c.418G gt A is more prevalent in celiac disease patients in the Serbian population (P  lt  0.01), while NCK2 c.745_746delAAinsG is less prevalent in celiac disease patients rather than healthy individuals of Greek descent (P = 0. 03). SLC9A4 c.1919G gt A and KIAA1109 c.2933T gt C and c.4268_4269delCCinsTA were more abundant in patients; nevertheless, they failed to show statistical significance. Conclusions: The next-generation sequencing-based family genomics approach described herein may serve as a paradigm towards the identification of novel functional variants with the aim of understanding complex disease pathobiology.",
publisher = "Biomed Central Ltd, London",
journal = "Human Genomics",
title = "Novel genetic risk variants for pediatric celiac disease",
volume = "10",
doi = "10.1186/s40246-016-0091-1"
}

Balasopoulou, A., Stanković, B., Panagiotara, A., Nikčević, G., Peters, B. A., John, A., Mendrinou, E., Stratopoulos, A., Legaki, A. I., Stathakopoulou, V., Tsolia, A., Govaris, N., Govari, S., Zagoriti, Z., Poulas, K., Kanariou, M., Constantinidou, N., Krini, M., Spanou, K., Radlović, N., Ali, B. R., Borg, J., Drmanac, R., Chrousos, G., Pavlović, S., Roma, E., Zukić, B., Patrinos, G. P.,& Katsila, T.. (2016). Novel genetic risk variants for pediatric celiac disease. in Human Genomics
Biomed Central Ltd, London., 10.
https://doi.org/10.1186/s40246-016-0091-1

Balasopoulou A, Stanković B, Panagiotara A, Nikčević G, Peters BA, John A, Mendrinou E, Stratopoulos A, Legaki AI, Stathakopoulou V, Tsolia A, Govaris N, Govari S, Zagoriti Z, Poulas K, Kanariou M, Constantinidou N, Krini M, Spanou K, Radlović N, Ali BR, Borg J, Drmanac R, Chrousos G, Pavlović S, Roma E, Zukić B, Patrinos GP, Katsila T. Novel genetic risk variants for pediatric celiac disease. in Human Genomics. 2016;10.
doi:10.1186/s40246-016-0091-1 .

Balasopoulou, Angeliki, Stanković, Biljana, Panagiotara, Angeliki, Nikčević, Gordana, Peters, Brock A., John, Anne, Mendrinou, Effrosyni, Stratopoulos, Apostolos, Legaki, Aigli Ioanna, Stathakopoulou, Vasiliki, Tsolia, Aristoniki, Govaris, Nikolaos, Govari, Sofia, Zagoriti, Zoi, Poulas, Konstantinos, Kanariou, Maria, Constantinidou, Nikki, Krini, Maro, Spanou, Kleopatra, Radlović, Nedeljko, Ali, Bassam R., Borg, Joseph, Drmanac, Radoje, Chrousos, George, Pavlović, Sonja, Roma, Eleftheria, Zukić, Branka, Patrinos, George P., Katsila, Theodora, "Novel genetic risk variants for pediatric celiac disease" in Human Genomics, 10 (2016),
https://doi.org/10.1186/s40246-016-0091-1 . .

TY  - JOUR
AU  - Jasnić, Jovana
AU  - Lazić, Andrijana
AU  - Milivojević, Milena
AU  - Mojsin, Marija
AU  - Nikčević, Gordana
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/807
AB  - Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.
PB  - Sage Publications Ltd, London
T2  - ATLA-Alternatives To Laboratory Animals
T1  - Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation
EP  - 18
IS  - 1
SP  - 9
VL  - 43
DO  - 10.1177/026119291504300105
ER  - 

@article{
author = "Jasnić, Jovana and Lazić, Andrijana and Milivojević, Milena and Mojsin, Marija and Nikčević, Gordana",
year = "2015",
abstract = "Serum is generally regarded as an essential component of many eukaryotic cell culture media, despite the fact that serum composition varies greatly and may be the source of a wide range of artefacts. The objective of this study was to assess serum-free growth conditions for the human embryonal carcinoma cell line, NT2/D1. These cells greatly resemble embryonic stem cells. In the presence of retinoic acid (RA), NT2/D1 cells irreversibly differentiate along the neuronal lineage. We have previously shown that the early phases of neural induction of these cells by RA involve the up-regulation of SOX3 gene expression. Our goal was to compare RA-induced differentiation of NT2/D1 cells in serum-containing and serum-free media, by using SOX3 protein levels as a marker of differentiation. We found that NT2/D1 cells can be successfully grown under serum-free conditions, and that the presence or absence of serum does not affect the level of SOX3 protein after a 48-hour RA induction. However, six days of RA treatment resulted in a marked increase in SOX3 protein levels in serum-free media compared to serum-containing media, indicating that serum might have an inhibitory effect on the expression of this neural differentiation marker. This finding is important for both basic and translational studies that hope to exploit cell culture conditions that are free of animal-derived products.",
publisher = "Sage Publications Ltd, London",
journal = "ATLA-Alternatives To Laboratory Animals",
title = "Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation",
pages = "18-9",
number = "1",
volume = "43",
doi = "10.1177/026119291504300105"
}

Jasnić, J., Lazić, A., Milivojević, M., Mojsin, M.,& Nikčević, G.. (2015). Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. in ATLA-Alternatives To Laboratory Animals
Sage Publications Ltd, London., 43(1), 9-18.
https://doi.org/10.1177/026119291504300105

Jasnić J, Lazić A, Milivojević M, Mojsin M, Nikčević G. Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation. in ATLA-Alternatives To Laboratory Animals. 2015;43(1):9-18.
doi:10.1177/026119291504300105 .

Jasnić, Jovana, Lazić, Andrijana, Milivojević, Milena, Mojsin, Marija, Nikčević, Gordana, "Human Embryonal Carcinoma Cells in Serum-free Conditions as an In Vitro Model System of Neural Differentiation" in ATLA-Alternatives To Laboratory Animals, 43, no. 1 (2015):9-18,
https://doi.org/10.1177/026119291504300105 . .

TY  - JOUR
AU  - Stanković, Biljana
AU  - Dragasević, Sanja
AU  - Popović, Dragan
AU  - Zukić, Branka
AU  - Kotur, Nikola
AU  - Sokić-Milutinović, Aleksandra
AU  - Alempijević, Tamara
AU  - Lukić, Snežana
AU  - Milosavljević, Tomica
AU  - Nikčević, Gordana
AU  - Pavlović, Sonja
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/810
AB  - OBJECTIVE: Research on inflammatory bowel disease (IBD) has highlighted genes involved in the regulation of inflammatory responses as contributors to disease pathogenesis. This study aimed to evaluate the associations between IBD and variations in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta and IL-1RN genes, and to use the genetic data obtained in predictive modeling. METHODS: A total of 167 IBD patients and 101 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Using the genotype data attained as the input to various classification algorithms, IBD prediction models were designed. The area under the receiver operating characteristic curve (AUROC) was used to measure their performance. RESULTS: Significant associations were found between Crohn's disease (CD) and minor NOD2 variants, as well as TLR4 299Gly, TNF-alpha G-308A, IL-6 G-174C and IL-1RN VNTR A2 variants, while ulcerative colitis (UC) was associated only with IL-1RN VNTR A2 variants. CD and UC showed highly significant difference in the allelic distribution of TNF-alpha G-308A, where the A allele was found to be related to CD, and the G allele to UC. A combined effect of patients' gender and TLR4 variants was observed among CD patients. When all analyzed genotype and gender data were used, prediction performance achieved a maximum AUROC of 0.690 for CD and 0.601 for UC dataset. CONCLUSION: Variations in the genes involved in immune regulation are genetic factors of importance in IBD susceptibility that could potentially be used as predictors of disease development.
PB  - Wiley, Hoboken
T2  - Journal of Digestive Diseases
T1  - Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence
EP  - 733
IS  - 12
SP  - 723
VL  - 16
DO  - 10.1111/1751-2980.12281
ER  - 

@article{
author = "Stanković, Biljana and Dragasević, Sanja and Popović, Dragan and Zukić, Branka and Kotur, Nikola and Sokić-Milutinović, Aleksandra and Alempijević, Tamara and Lukić, Snežana and Milosavljević, Tomica and Nikčević, Gordana and Pavlović, Sonja",
year = "2015",
abstract = "OBJECTIVE: Research on inflammatory bowel disease (IBD) has highlighted genes involved in the regulation of inflammatory responses as contributors to disease pathogenesis. This study aimed to evaluate the associations between IBD and variations in NOD2, TLR4, TNF-alpha, IL-6, IL-1 beta and IL-1RN genes, and to use the genetic data obtained in predictive modeling. METHODS: A total of 167 IBD patients and 101 healthy controls were genotyped by polymerase chain reaction-restriction fragment length polymorphism. Using the genotype data attained as the input to various classification algorithms, IBD prediction models were designed. The area under the receiver operating characteristic curve (AUROC) was used to measure their performance. RESULTS: Significant associations were found between Crohn's disease (CD) and minor NOD2 variants, as well as TLR4 299Gly, TNF-alpha G-308A, IL-6 G-174C and IL-1RN VNTR A2 variants, while ulcerative colitis (UC) was associated only with IL-1RN VNTR A2 variants. CD and UC showed highly significant difference in the allelic distribution of TNF-alpha G-308A, where the A allele was found to be related to CD, and the G allele to UC. A combined effect of patients' gender and TLR4 variants was observed among CD patients. When all analyzed genotype and gender data were used, prediction performance achieved a maximum AUROC of 0.690 for CD and 0.601 for UC dataset. CONCLUSION: Variations in the genes involved in immune regulation are genetic factors of importance in IBD susceptibility that could potentially be used as predictors of disease development.",
publisher = "Wiley, Hoboken",
journal = "Journal of Digestive Diseases",
title = "Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence",
pages = "733-723",
number = "12",
volume = "16",
doi = "10.1111/1751-2980.12281"
}

Stanković, B., Dragasević, S., Popović, D., Zukić, B., Kotur, N., Sokić-Milutinović, A., Alempijević, T., Lukić, S., Milosavljević, T., Nikčević, G.,& Pavlović, S.. (2015). Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence. in Journal of Digestive Diseases
Wiley, Hoboken., 16(12), 723-733.
https://doi.org/10.1111/1751-2980.12281

Stanković B, Dragasević S, Popović D, Zukić B, Kotur N, Sokić-Milutinović A, Alempijević T, Lukić S, Milosavljević T, Nikčević G, Pavlović S. Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence. in Journal of Digestive Diseases. 2015;16(12):723-733.
doi:10.1111/1751-2980.12281 .

Stanković, Biljana, Dragasević, Sanja, Popović, Dragan, Zukić, Branka, Kotur, Nikola, Sokić-Milutinović, Aleksandra, Alempijević, Tamara, Lukić, Snežana, Milosavljević, Tomica, Nikčević, Gordana, Pavlović, Sonja, "Variations in inflammatory genes as molecular markers for prediction of inflammatory bowel disease occurrence" in Journal of Digestive Diseases, 16, no. 12 (2015):723-733,
https://doi.org/10.1111/1751-2980.12281 . .

TY  - JOUR
AU  - Srzentić Dražilov, Sanja
AU  - Nikčević, Gordana
AU  - Spasovski, Dusko
AU  - Bascarević, Zoran
AU  - Zivković, Zorica
AU  - Terzić-Supić, Zorica
AU  - Matanović, Dragana
AU  - Đorđević, Valentina
AU  - Pavlović, Sonja
AU  - Spasovski, Vesna
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/796
AB  - Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients.
PB  - Springer, New York
T2  - European Journal of Pediatrics
T1  - Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience
EP  - 1092
IS  - 8
SP  - 1085
VL  - 174
DO  - 10.1007/s00431-015-2510-z
ER  - 

@article{
author = "Srzentić Dražilov, Sanja and Nikčević, Gordana and Spasovski, Dusko and Bascarević, Zoran and Zivković, Zorica and Terzić-Supić, Zorica and Matanović, Dragana and Đorđević, Valentina and Pavlović, Sonja and Spasovski, Vesna",
year = "2015",
abstract = "Perthes disease is one of the most common forms of pediatric femoral head osteonecrosis with an unknown etiology. Coagulation factors were the first genetic factors suspected to have a role in the pathogenesis of this disease, but studies showed inconsistent results. It is described that inflammation is present during early stages of Perthes disease, but its genetic aspect has not been studied extensively. Little is known regarding the status of apoptotic factors during the repair process that leads to the occurrence of hip deformity in patients. Therefore, the aim of this study was to analyze major mediators involved in coagulation, inflammation, and apoptotic processes as possible causative factors of Perthes disease. The study cohort consisted of 37 patients. Gene variants of TNF-alpha, FV, FII, and MTHFR genes were determined by PCR-RFLP, while IL-3 and PAI-1 were genotyped by direct sequencing. The expression level of Bax, Bcl-2, Bcl2L12, Fas and FasL was analyzed by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) technique. Our results showed a significantly increased level of expression of pro-apoptotic factor Bax along with significantly higher Bax/Bcl-2 ratio in the patient group. Conclusion: The results presented indicate that apoptosis could be one of the factors contributing to the lack of balanced bone remodeling process in Perthes patients.",
publisher = "Springer, New York",
journal = "European Journal of Pediatrics",
title = "Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience",
pages = "1092-1085",
number = "8",
volume = "174",
doi = "10.1007/s00431-015-2510-z"
}

Srzentić Dražilov, S., Nikčević, G., Spasovski, D., Bascarević, Z., Zivković, Z., Terzić-Supić, Z., Matanović, D., Đorđević, V., Pavlović, S.,& Spasovski, V.. (2015). Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience. in European Journal of Pediatrics
Springer, New York., 174(8), 1085-1092.
https://doi.org/10.1007/s00431-015-2510-z

Srzentić Dražilov S, Nikčević G, Spasovski D, Bascarević Z, Zivković Z, Terzić-Supić Z, Matanović D, Đorđević V, Pavlović S, Spasovski V. Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience. in European Journal of Pediatrics. 2015;174(8):1085-1092.
doi:10.1007/s00431-015-2510-z .

Srzentić Dražilov, Sanja, Nikčević, Gordana, Spasovski, Dusko, Bascarević, Zoran, Zivković, Zorica, Terzić-Supić, Zorica, Matanović, Dragana, Đorđević, Valentina, Pavlović, Sonja, Spasovski, Vesna, "Predictive genetic markers of coagulation, inflammation and apoptosis in Perthes disease-Serbian experience" in European Journal of Pediatrics, 174, no. 8 (2015):1085-1092,
https://doi.org/10.1007/s00431-015-2510-z . .

TY  - JOUR
AU  - Srzentić Dražilov, Sanja
AU  - Spasovski, Vesna
AU  - Spasovski, Duško
AU  - Živković, Zorica
AU  - Matanović, Dragana
AU  - Baščarević, Zoran
AU  - Šupić-Terzić, Zorica
AU  - Stojiljković, Maja
AU  - Karan-Đurašević, Teodora
AU  - Stanković, Biljana
AU  - Pavlović, Sonja
AU  - Nikčević, Gordana
AU  - Vukašinović, Zoran
PY  - 2014
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/752
AB  - Uvod Pertesova bolest je idiopatska avaskularna osteonekroza proksimalne epifize femura koja se javlja kod dece. Etiologija ove bolesti je nepoznata. Tokom razvoja Pertesove bolesti zastupljen je proces zapaljenja, za koji je pokazano da utiče na remodelovanje koštanog tkiva. Cilj rada S obzirom na to da genetički faktori koji utiču na proces zapaljenja dosad nisu ispitivani kod Pertesove bolesti, cilj ovog istraživanja je bio da se utvrdi povezanost učestalosti varijanti u genima koji učestvuju u inflamatornom odgovoru, TLR4 (engl. toll-like receptor 4) i IL-6 (interleukin 6), i ove bolesti. Metode rada Ispitano je 37 dece s Pertesovom bolešću i 50 zdravih osoba. Metodom PCR-RFLP određeni su polimorfizmi medijatora zapaljenja TLR4 (Asp299Gly, Thr399Ile) i IL-6 (G-174C, G-597A). Rezultati Pokazano je da su polimorfizmi IL-6 G-174C i G-597A u našem ispitivanju bili u potpunoj gametskoj neravnoteži vezanosti. U kontrolnoj grupi je bilo statistički značajno više nosilaca heterozigotnog genotipa IL-6 G-174C/G-597A u poređenju sa grupom ispitanika s Pertesovom bolešću (p=0,047; OR=2,49; 95% CI=1,00-6,21). Takođe, grupa bolesnika nije bila u Hardi- Vajnbergovoj ravnoteži za polimorfizme IL-6 G-174C/G-597A. Nije primećena statistički značajna razlika u raspodeli genotipova za polimorfizme analizirane u TLR4 genu. Raspodela genotipova među grupama bolesnika formiranih na osnovu uzrasta kada se bolest pojavila nije pokazala statistički značajnu povezanost s analiziranim polimorfizmima. Zaključak Naše istraživanje je pokazalo da su nosioci heterozigotnog genotipa za IL-6 G-174C/G-597A polimorfizme bili značajno češći u kontrolnoj grupi nego u grupi dece obolele od Pertesove bolesti. Na osnovu toga zaključili smo da je kod dece koja su nosioci heterozigotnog genotipa za ove polimorfizme manja verovatnoća za razvoj Pertesove bolesti nego kod nosilaca oba homozigotna genotipa.
AB  - Introduction Perthes disease is idiopathic avascular osteonecrosis of the hip in children, with unknown etiology. Inflammation is present during development of Perthes disease and it is known that this process influences bone remodeling. Objective Since genetic studies related to inflammation have not been performed in Perthes disease so far, the aim of this study was to analyze the association of frequencies of genetic variants of immune response genes, toll-like receptor 4 (TLR4) and interleukin-6 (IL-6), with this disease. Methods The study cohort consisted of 37 patients with Perthes disease and 50 healthy controls. Polymorphisms of well described inflammatory mediators: TLR4 (Asp299Gly, Thr399Ile) and IL-6 (G-174C, G- 597A) were determined by polymerase chain reaction restriction fragment length polymorphism method. Results IL-6 G-174C and G-597A polymorphisms were in complete linkage disequilibrium. A statistically significant increase of heterozygote subjects for IL-6 G-174C/G-597A was found in controls in comparison to Perthes patient group (p=0.047, OR=2.49, 95% CI=1.00-6.21). Also, the patient group for IL-6 G-174C/G- 597A polymorphisms was not in Hardy-Weinberg equilibrium. No statistically significant differences were found between patient and control groups for TLR4 analyzed polymorphisms. A stratified analysis by the age at disease onset also did not reveal any significant difference for all analyzed polymorphisms. Conclusion Our study revealed that heterozygote subjects for the IL-6 G-174C/G-597A polymorphisms were significantly overrepresented in the control group than in the Perthes patient group. Consequently, we concluded that children who are heterozygous for these polymorphisms have a lower chance of developing Perthes disease than carriers of both homozygote genotypes.
PB  - Srpsko lekarsko društvo, Beograd
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Povezanost varijanti u genima TLR4 i IL-6 s Pertesovom bolešću
T1  - Association of gene variants in TLR4 and IL-6 genes with Perthes disease
EP  - 456
IS  - 7-8
SP  - 450
VL  - 142
DO  - 10.2298/SARH1408450S
ER  - 

@article{
author = "Srzentić Dražilov, Sanja and Spasovski, Vesna and Spasovski, Duško and Živković, Zorica and Matanović, Dragana and Baščarević, Zoran and Šupić-Terzić, Zorica and Stojiljković, Maja and Karan-Đurašević, Teodora and Stanković, Biljana and Pavlović, Sonja and Nikčević, Gordana and Vukašinović, Zoran",
year = "2014",
abstract = "Uvod Pertesova bolest je idiopatska avaskularna osteonekroza proksimalne epifize femura koja se javlja kod dece. Etiologija ove bolesti je nepoznata. Tokom razvoja Pertesove bolesti zastupljen je proces zapaljenja, za koji je pokazano da utiče na remodelovanje koštanog tkiva. Cilj rada S obzirom na to da genetički faktori koji utiču na proces zapaljenja dosad nisu ispitivani kod Pertesove bolesti, cilj ovog istraživanja je bio da se utvrdi povezanost učestalosti varijanti u genima koji učestvuju u inflamatornom odgovoru, TLR4 (engl. toll-like receptor 4) i IL-6 (interleukin 6), i ove bolesti. Metode rada Ispitano je 37 dece s Pertesovom bolešću i 50 zdravih osoba. Metodom PCR-RFLP određeni su polimorfizmi medijatora zapaljenja TLR4 (Asp299Gly, Thr399Ile) i IL-6 (G-174C, G-597A). Rezultati Pokazano je da su polimorfizmi IL-6 G-174C i G-597A u našem ispitivanju bili u potpunoj gametskoj neravnoteži vezanosti. U kontrolnoj grupi je bilo statistički značajno više nosilaca heterozigotnog genotipa IL-6 G-174C/G-597A u poređenju sa grupom ispitanika s Pertesovom bolešću (p=0,047; OR=2,49; 95% CI=1,00-6,21). Takođe, grupa bolesnika nije bila u Hardi- Vajnbergovoj ravnoteži za polimorfizme IL-6 G-174C/G-597A. Nije primećena statistički značajna razlika u raspodeli genotipova za polimorfizme analizirane u TLR4 genu. Raspodela genotipova među grupama bolesnika formiranih na osnovu uzrasta kada se bolest pojavila nije pokazala statistički značajnu povezanost s analiziranim polimorfizmima. Zaključak Naše istraživanje je pokazalo da su nosioci heterozigotnog genotipa za IL-6 G-174C/G-597A polimorfizme bili značajno češći u kontrolnoj grupi nego u grupi dece obolele od Pertesove bolesti. Na osnovu toga zaključili smo da je kod dece koja su nosioci heterozigotnog genotipa za ove polimorfizme manja verovatnoća za razvoj Pertesove bolesti nego kod nosilaca oba homozigotna genotipa., Introduction Perthes disease is idiopathic avascular osteonecrosis of the hip in children, with unknown etiology. Inflammation is present during development of Perthes disease and it is known that this process influences bone remodeling. Objective Since genetic studies related to inflammation have not been performed in Perthes disease so far, the aim of this study was to analyze the association of frequencies of genetic variants of immune response genes, toll-like receptor 4 (TLR4) and interleukin-6 (IL-6), with this disease. Methods The study cohort consisted of 37 patients with Perthes disease and 50 healthy controls. Polymorphisms of well described inflammatory mediators: TLR4 (Asp299Gly, Thr399Ile) and IL-6 (G-174C, G- 597A) were determined by polymerase chain reaction restriction fragment length polymorphism method. Results IL-6 G-174C and G-597A polymorphisms were in complete linkage disequilibrium. A statistically significant increase of heterozygote subjects for IL-6 G-174C/G-597A was found in controls in comparison to Perthes patient group (p=0.047, OR=2.49, 95% CI=1.00-6.21). Also, the patient group for IL-6 G-174C/G- 597A polymorphisms was not in Hardy-Weinberg equilibrium. No statistically significant differences were found between patient and control groups for TLR4 analyzed polymorphisms. A stratified analysis by the age at disease onset also did not reveal any significant difference for all analyzed polymorphisms. Conclusion Our study revealed that heterozygote subjects for the IL-6 G-174C/G-597A polymorphisms were significantly overrepresented in the control group than in the Perthes patient group. Consequently, we concluded that children who are heterozygous for these polymorphisms have a lower chance of developing Perthes disease than carriers of both homozygote genotypes.",
publisher = "Srpsko lekarsko društvo, Beograd",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Povezanost varijanti u genima TLR4 i IL-6 s Pertesovom bolešću, Association of gene variants in TLR4 and IL-6 genes with Perthes disease",
pages = "456-450",
number = "7-8",
volume = "142",
doi = "10.2298/SARH1408450S"
}

Srzentić Dražilov, S., Spasovski, V., Spasovski, D., Živković, Z., Matanović, D., Baščarević, Z., Šupić-Terzić, Z., Stojiljković, M., Karan-Đurašević, T., Stanković, B., Pavlović, S., Nikčević, G.,& Vukašinović, Z.. (2014). Povezanost varijanti u genima TLR4 i IL-6 s Pertesovom bolešću. in Srpski arhiv za celokupno lekarstvo
Srpsko lekarsko društvo, Beograd., 142(7-8), 450-456.
https://doi.org/10.2298/SARH1408450S

Srzentić Dražilov S, Spasovski V, Spasovski D, Živković Z, Matanović D, Baščarević Z, Šupić-Terzić Z, Stojiljković M, Karan-Đurašević T, Stanković B, Pavlović S, Nikčević G, Vukašinović Z. Povezanost varijanti u genima TLR4 i IL-6 s Pertesovom bolešću. in Srpski arhiv za celokupno lekarstvo. 2014;142(7-8):450-456.
doi:10.2298/SARH1408450S .

Srzentić Dražilov, Sanja, Spasovski, Vesna, Spasovski, Duško, Živković, Zorica, Matanović, Dragana, Baščarević, Zoran, Šupić-Terzić, Zorica, Stojiljković, Maja, Karan-Đurašević, Teodora, Stanković, Biljana, Pavlović, Sonja, Nikčević, Gordana, Vukašinović, Zoran, "Povezanost varijanti u genima TLR4 i IL-6 s Pertesovom bolešću" in Srpski arhiv za celokupno lekarstvo, 142, no. 7-8 (2014):450-456,
https://doi.org/10.2298/SARH1408450S . .

TY  - JOUR
AU  - Spasovski, Vesna
AU  - Tošić, Nataša
AU  - Nikčević, Gordana
AU  - Stojiljković, Maja
AU  - Zukić, Branka
AU  - Ugrin, Milena
AU  - Karan-Đurašević, Teodora
AU  - Srzentić Dražilov, Sanja
AU  - Colović, Milica
AU  - Pavlović, Sonja
PY  - 2013
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/619
AB  - The expression of Janus kinase 2 (JAK2) gene is altered in myeloproliferative neoplasms (MPN) and the regulation of transcription could be a mechanism that modulates JAK2 gene expression. We analyzed the transcriptional potential of single-nucleotide polymorphism (SNP) rs12343867 T  gt  C in JAK2 intron 14, tagging 46/1 haplotype, and its influence on JAK2 gene expression. Functional analysis of JAK2 intron 14 was performed using the pBLCAT5 reporter system in K562 cells. Identification of the proteins binding to the intron 14 regulatory element was accomplished by electrophoretic mobility shift assay (EMSA) and supershift assays. Quantification of the expression of JAK2 gene in a cohort of 51 MPN patients and 12 healthy controls was performed by real-time quantitative polymerase chain reaction (RQ-PCR). Functional analysis revealed that the intronic DNA element harboring SNP rs12343867 T  gt  C acts as a transcriptional repressor in vitro. The repressor activity was significantly attenuated by the presence of nucleotide C. Supershift analysis showed the enrolment of transcriptional factor Meis1 in this process. RQ-PCR experiments showed increased JAK2 expression in patients with the JAK2V617F mutation, with a significant difference between essential thrombocythemia (ET), polycythemia vera (PV), and myelofibrosis (MF) patients. SNP rs12343867 showed no statistically significant influence on the expression of JAK2 gene in MNP patients.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Applied Genetics
T1  - The influence of novel transcriptional regulatory element in intron 14 on the expression of Janus kinase 2 gene in myeloproliferative neoplasms
EP  - 26
IS  - 1
SP  - 21
VL  - 54
DO  - 10.1007/s13353-012-0125-x
ER  - 

@article{
author = "Spasovski, Vesna and Tošić, Nataša and Nikčević, Gordana and Stojiljković, Maja and Zukić, Branka and Ugrin, Milena and Karan-Đurašević, Teodora and Srzentić Dražilov, Sanja and Colović, Milica and Pavlović, Sonja",
year = "2013",
abstract = "The expression of Janus kinase 2 (JAK2) gene is altered in myeloproliferative neoplasms (MPN) and the regulation of transcription could be a mechanism that modulates JAK2 gene expression. We analyzed the transcriptional potential of single-nucleotide polymorphism (SNP) rs12343867 T  gt  C in JAK2 intron 14, tagging 46/1 haplotype, and its influence on JAK2 gene expression. Functional analysis of JAK2 intron 14 was performed using the pBLCAT5 reporter system in K562 cells. Identification of the proteins binding to the intron 14 regulatory element was accomplished by electrophoretic mobility shift assay (EMSA) and supershift assays. Quantification of the expression of JAK2 gene in a cohort of 51 MPN patients and 12 healthy controls was performed by real-time quantitative polymerase chain reaction (RQ-PCR). Functional analysis revealed that the intronic DNA element harboring SNP rs12343867 T  gt  C acts as a transcriptional repressor in vitro. The repressor activity was significantly attenuated by the presence of nucleotide C. Supershift analysis showed the enrolment of transcriptional factor Meis1 in this process. RQ-PCR experiments showed increased JAK2 expression in patients with the JAK2V617F mutation, with a significant difference between essential thrombocythemia (ET), polycythemia vera (PV), and myelofibrosis (MF) patients. SNP rs12343867 showed no statistically significant influence on the expression of JAK2 gene in MNP patients.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Applied Genetics",
title = "The influence of novel transcriptional regulatory element in intron 14 on the expression of Janus kinase 2 gene in myeloproliferative neoplasms",
pages = "26-21",
number = "1",
volume = "54",
doi = "10.1007/s13353-012-0125-x"
}

Spasovski, V., Tošić, N., Nikčević, G., Stojiljković, M., Zukić, B., Ugrin, M., Karan-Đurašević, T., Srzentić Dražilov, S., Colović, M.,& Pavlović, S.. (2013). The influence of novel transcriptional regulatory element in intron 14 on the expression of Janus kinase 2 gene in myeloproliferative neoplasms. in Journal of Applied Genetics
Springer Heidelberg, Heidelberg., 54(1), 21-26.
https://doi.org/10.1007/s13353-012-0125-x

Spasovski V, Tošić N, Nikčević G, Stojiljković M, Zukić B, Ugrin M, Karan-Đurašević T, Srzentić Dražilov S, Colović M, Pavlović S. The influence of novel transcriptional regulatory element in intron 14 on the expression of Janus kinase 2 gene in myeloproliferative neoplasms. in Journal of Applied Genetics. 2013;54(1):21-26.
doi:10.1007/s13353-012-0125-x .

Spasovski, Vesna, Tošić, Nataša, Nikčević, Gordana, Stojiljković, Maja, Zukić, Branka, Ugrin, Milena, Karan-Đurašević, Teodora, Srzentić Dražilov, Sanja, Colović, Milica, Pavlović, Sonja, "The influence of novel transcriptional regulatory element in intron 14 on the expression of Janus kinase 2 gene in myeloproliferative neoplasms" in Journal of Applied Genetics, 54, no. 1 (2013):21-26,
https://doi.org/10.1007/s13353-012-0125-x . .

TY  - JOUR
AU  - Stojiljković, Maja
AU  - Fazlagić, Amira
AU  - Dokmanović-Krivokapić, Lidija
AU  - Nikčević, Gordana
AU  - Patrinos, George P.
AU  - Pavlović, Sonja
AU  - Zukić, Branka
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/570
AB  - The Golden Helix Pharmacogenomics Days are international scientific meetings aiming to educate healthcare professionals and biomedical scientists about pharmacogenomics and personalized medicine. In this meeting report, we provide an overview of the scientific lectures and the topics discussed during the 6th Golden Helix Pharmacogenomics Day that was held in Belgrade, Serbia last June 5, 2012. The scientific program included lectures by the local and international speakers from Europe and the United States.
PB  - Biomed Central Ltd, London
T2  - Human Genomics
T1  - 6th Golden Helix Pharmacogenomics Day: pharmacogenomics and individualized therapy
VL  - 6
DO  - 10.1186/1479-7364-6-19
ER  - 

@article{
author = "Stojiljković, Maja and Fazlagić, Amira and Dokmanović-Krivokapić, Lidija and Nikčević, Gordana and Patrinos, George P. and Pavlović, Sonja and Zukić, Branka",
year = "2012",
abstract = "The Golden Helix Pharmacogenomics Days are international scientific meetings aiming to educate healthcare professionals and biomedical scientists about pharmacogenomics and personalized medicine. In this meeting report, we provide an overview of the scientific lectures and the topics discussed during the 6th Golden Helix Pharmacogenomics Day that was held in Belgrade, Serbia last June 5, 2012. The scientific program included lectures by the local and international speakers from Europe and the United States.",
publisher = "Biomed Central Ltd, London",
journal = "Human Genomics",
title = "6th Golden Helix Pharmacogenomics Day: pharmacogenomics and individualized therapy",
volume = "6",
doi = "10.1186/1479-7364-6-19"
}

Stojiljković, M., Fazlagić, A., Dokmanović-Krivokapić, L., Nikčević, G., Patrinos, G. P., Pavlović, S.,& Zukić, B.. (2012). 6th Golden Helix Pharmacogenomics Day: pharmacogenomics and individualized therapy. in Human Genomics
Biomed Central Ltd, London., 6.
https://doi.org/10.1186/1479-7364-6-19

Stojiljković M, Fazlagić A, Dokmanović-Krivokapić L, Nikčević G, Patrinos GP, Pavlović S, Zukić B. 6th Golden Helix Pharmacogenomics Day: pharmacogenomics and individualized therapy. in Human Genomics. 2012;6.
doi:10.1186/1479-7364-6-19 .

Stojiljković, Maja, Fazlagić, Amira, Dokmanović-Krivokapić, Lidija, Nikčević, Gordana, Patrinos, George P., Pavlović, Sonja, Zukić, Branka, "6th Golden Helix Pharmacogenomics Day: pharmacogenomics and individualized therapy" in Human Genomics, 6 (2012),
https://doi.org/10.1186/1479-7364-6-19 . .

TY  - JOUR
AU  - Karan-Đurašević, Teodora
AU  - Palibrk, Vuk
AU  - Kostić, Tatjana
AU  - Spasovski, Vesna
AU  - Nikčević, Gordana
AU  - Srzentić Dražilov, Sanja
AU  - Colović, Milica
AU  - Čolović, Nataša
AU  - Vidović, Ana
AU  - Antić, Darko
AU  - Mihaljević, Biljana
AU  - Pavlović, Sonja
AU  - Tošić, Nataša
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/565
AB  - The mutational status and configuration of immunoglobulin heavy variable (IGHV) gene rearrangements was analyzed in 85 Serbian patients with chronic lymphocytic leukemia (CLL). We found that 55.3% of cases belonged to mutated and 44.7% to unmutated CLL, progressive disease predominating in the unmutated subset. IGHV gene use resembled that obtained for Mediterranean countries, except for underrepresentation of the IGHV4 subgroup in our cohort. Background: Chronic lymphocytic leukemia (CLL) results from the clonal expansion of mature B lymphocytes and is characterized by extreme clinical heterogeneity. One of the most reliable prognostic markers in chronic lymphocytic leukemia (CLL) is the mutational status of immunoglobulin heavy variable (IGHV) genes, which defines 2 subsets, mutated CLL (M-CLL) and unmutated CLL (U-CLL), with different clinical courses. Biased IGHV gene use between M-CLL and U-CLL clones, as well as population differences in the IGHV gene repertoire have been reported. Patients and Methods: In this study, mutational status and configuration of IGHV-IGHD-IGHJ rearrangements in 85 Serbian patients were analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing methodology. Results: We found that 55.3% of cases belonged to M-CLL and 44.7% belonged to U-CLL, with progressive disease predominating in the unmutated subset. Most frequently expressed was the IGHV3 subgroup (55.7%), followed by IGHV1 (27.3%), IGHV4 (12.5%), IGHV5 (2.3%), IGHV2 (1.1%), and IGHV6 (1.1%). The distribution of IGHD subgroups was as follows: IGHD3, 39.1%; IGHD2, 21.8%; IGHD6, 12.6%; IGHD1, 10.3%; IGHD4, 8%; IGHD5, 6.9%; and IGHD7, 1.1%. The most frequent IGHJ gene was IGHJ4 (48.9%), followed by IGHJ6 (28.4%), IGHJ3 (11.4%), and IGHJ5 (11.4%). In 15.3% of cases, heavy complementarity-determining region 3 (VH CDR3) amino acid sequences could be assigned to previously defined stereotyped clusters. Conclusions: Our study showed a strong correlation between IGHV gene mutational status and clinical course of CLL. IGHV gene use was comparable to that obtained for Mediterranean countries, with the exception of the IGHV4 subgroup, which was underrepresented in our cohort. Clinical Lymphoma, Myeloma & Leukemia, Vol. 12, No. 4, 252-60
PB  - CIG Media Group, Lp, Dallas
T2  - Clinical Lymphoma Myeloma & Leukemia
T1  - Mutational Status and Gene Repertoire of IGHV-IGHD-IGHJ Rearrangements in Serbian Patients With Chronic Lymphocytic Leukemia
EP  - 260
IS  - 4
SP  - 252
VL  - 12
DO  - 10.1016/j.clml.2012.03.005
ER  - 

@article{
author = "Karan-Đurašević, Teodora and Palibrk, Vuk and Kostić, Tatjana and Spasovski, Vesna and Nikčević, Gordana and Srzentić Dražilov, Sanja and Colović, Milica and Čolović, Nataša and Vidović, Ana and Antić, Darko and Mihaljević, Biljana and Pavlović, Sonja and Tošić, Nataša",
year = "2012",
abstract = "The mutational status and configuration of immunoglobulin heavy variable (IGHV) gene rearrangements was analyzed in 85 Serbian patients with chronic lymphocytic leukemia (CLL). We found that 55.3% of cases belonged to mutated and 44.7% to unmutated CLL, progressive disease predominating in the unmutated subset. IGHV gene use resembled that obtained for Mediterranean countries, except for underrepresentation of the IGHV4 subgroup in our cohort. Background: Chronic lymphocytic leukemia (CLL) results from the clonal expansion of mature B lymphocytes and is characterized by extreme clinical heterogeneity. One of the most reliable prognostic markers in chronic lymphocytic leukemia (CLL) is the mutational status of immunoglobulin heavy variable (IGHV) genes, which defines 2 subsets, mutated CLL (M-CLL) and unmutated CLL (U-CLL), with different clinical courses. Biased IGHV gene use between M-CLL and U-CLL clones, as well as population differences in the IGHV gene repertoire have been reported. Patients and Methods: In this study, mutational status and configuration of IGHV-IGHD-IGHJ rearrangements in 85 Serbian patients were analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR) and sequencing methodology. Results: We found that 55.3% of cases belonged to M-CLL and 44.7% belonged to U-CLL, with progressive disease predominating in the unmutated subset. Most frequently expressed was the IGHV3 subgroup (55.7%), followed by IGHV1 (27.3%), IGHV4 (12.5%), IGHV5 (2.3%), IGHV2 (1.1%), and IGHV6 (1.1%). The distribution of IGHD subgroups was as follows: IGHD3, 39.1%; IGHD2, 21.8%; IGHD6, 12.6%; IGHD1, 10.3%; IGHD4, 8%; IGHD5, 6.9%; and IGHD7, 1.1%. The most frequent IGHJ gene was IGHJ4 (48.9%), followed by IGHJ6 (28.4%), IGHJ3 (11.4%), and IGHJ5 (11.4%). In 15.3% of cases, heavy complementarity-determining region 3 (VH CDR3) amino acid sequences could be assigned to previously defined stereotyped clusters. Conclusions: Our study showed a strong correlation between IGHV gene mutational status and clinical course of CLL. IGHV gene use was comparable to that obtained for Mediterranean countries, with the exception of the IGHV4 subgroup, which was underrepresented in our cohort. Clinical Lymphoma, Myeloma & Leukemia, Vol. 12, No. 4, 252-60",
publisher = "CIG Media Group, Lp, Dallas",
journal = "Clinical Lymphoma Myeloma & Leukemia",
title = "Mutational Status and Gene Repertoire of IGHV-IGHD-IGHJ Rearrangements in Serbian Patients With Chronic Lymphocytic Leukemia",
pages = "260-252",
number = "4",
volume = "12",
doi = "10.1016/j.clml.2012.03.005"
}

Karan-Đurašević, T., Palibrk, V., Kostić, T., Spasovski, V., Nikčević, G., Srzentić Dražilov, S., Colović, M., Čolović, N., Vidović, A., Antić, D., Mihaljević, B., Pavlović, S.,& Tošić, N.. (2012). Mutational Status and Gene Repertoire of IGHV-IGHD-IGHJ Rearrangements in Serbian Patients With Chronic Lymphocytic Leukemia. in Clinical Lymphoma Myeloma & Leukemia
CIG Media Group, Lp, Dallas., 12(4), 252-260.
https://doi.org/10.1016/j.clml.2012.03.005

Karan-Đurašević T, Palibrk V, Kostić T, Spasovski V, Nikčević G, Srzentić Dražilov S, Colović M, Čolović N, Vidović A, Antić D, Mihaljević B, Pavlović S, Tošić N. Mutational Status and Gene Repertoire of IGHV-IGHD-IGHJ Rearrangements in Serbian Patients With Chronic Lymphocytic Leukemia. in Clinical Lymphoma Myeloma & Leukemia. 2012;12(4):252-260.
doi:10.1016/j.clml.2012.03.005 .

Karan-Đurašević, Teodora, Palibrk, Vuk, Kostić, Tatjana, Spasovski, Vesna, Nikčević, Gordana, Srzentić Dražilov, Sanja, Colović, Milica, Čolović, Nataša, Vidović, Ana, Antić, Darko, Mihaljević, Biljana, Pavlović, Sonja, Tošić, Nataša, "Mutational Status and Gene Repertoire of IGHV-IGHD-IGHJ Rearrangements in Serbian Patients With Chronic Lymphocytic Leukemia" in Clinical Lymphoma Myeloma & Leukemia, 12, no. 4 (2012):252-260,
https://doi.org/10.1016/j.clml.2012.03.005 . .

TY  - JOUR
AU  - Kuzmanović, Milos
AU  - Tošić, Nataša
AU  - Čolović, Nataša
AU  - Karan-Đurašević, Teodora
AU  - Spasovski, Vesna
AU  - Ugrin, Milena
AU  - Nikčević, Gordana
AU  - Suvajdžić-Vuković, Nada
AU  - Tomin, Dragica
AU  - Vidović, Ana
AU  - Virijević, Marijana
AU  - Pavlović, Sonja
AU  - Colović, Milica
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/609
AB  - Based on current findings, the presence of NPM1 mutations in acute myeloid leukemia (AML) patients is associated with an increased probability of complete remission (CR) and better overall survival (OS). We determined the incidence and prognostic relevance of NPM1 mutations, their association with FLT3 and IDH mutations, and other clinical characteristics in Serbian adult AML patients. Samples from 111 adult de novo AML patients, including 73 AML cases with a normal karyotype (NK-AML), were studied. NPM1, FLT3, and IDH mutations were detected by PCR and direct sequencing. NPM1 mutations were detected in 22.5% of patients. The presence of NPM1 mutations predicted a low CR rate and shorter OS. NPM1 mutations showed an association with both FLT3 and IDH mutations. Survival analysis based on NPM1/FLT3 mutational status revealed a lower OS for NPM1(+)/FLT3(-) compared to the NPM1(-)/FLT3(-) group in NK-AML patients. The lack of impact or unfavorable prognostic effect of NPM1 mutations found in this study can be assigned to a small cohort of analyzed AML patients, as can the presence of FLT3 and IDH mutations or other genetic lesions that cooperate with NPM1 mutations influencing prognosis.
PB  - Karger, Basel
T2  - Acta Haematologica
T1  - Prognostic Impact of NPM1 Mutations in Serbian Adult Patients with Acute Myeloid Leukemia
EP  - 212
IS  - 4
SP  - 203
VL  - 128
DO  - 10.1159/000339506
ER  - 

@article{
author = "Kuzmanović, Milos and Tošić, Nataša and Čolović, Nataša and Karan-Đurašević, Teodora and Spasovski, Vesna and Ugrin, Milena and Nikčević, Gordana and Suvajdžić-Vuković, Nada and Tomin, Dragica and Vidović, Ana and Virijević, Marijana and Pavlović, Sonja and Colović, Milica",
year = "2012",
abstract = "Based on current findings, the presence of NPM1 mutations in acute myeloid leukemia (AML) patients is associated with an increased probability of complete remission (CR) and better overall survival (OS). We determined the incidence and prognostic relevance of NPM1 mutations, their association with FLT3 and IDH mutations, and other clinical characteristics in Serbian adult AML patients. Samples from 111 adult de novo AML patients, including 73 AML cases with a normal karyotype (NK-AML), were studied. NPM1, FLT3, and IDH mutations were detected by PCR and direct sequencing. NPM1 mutations were detected in 22.5% of patients. The presence of NPM1 mutations predicted a low CR rate and shorter OS. NPM1 mutations showed an association with both FLT3 and IDH mutations. Survival analysis based on NPM1/FLT3 mutational status revealed a lower OS for NPM1(+)/FLT3(-) compared to the NPM1(-)/FLT3(-) group in NK-AML patients. The lack of impact or unfavorable prognostic effect of NPM1 mutations found in this study can be assigned to a small cohort of analyzed AML patients, as can the presence of FLT3 and IDH mutations or other genetic lesions that cooperate with NPM1 mutations influencing prognosis.",
publisher = "Karger, Basel",
journal = "Acta Haematologica",
title = "Prognostic Impact of NPM1 Mutations in Serbian Adult Patients with Acute Myeloid Leukemia",
pages = "212-203",
number = "4",
volume = "128",
doi = "10.1159/000339506"
}

Kuzmanović, M., Tošić, N., Čolović, N., Karan-Đurašević, T., Spasovski, V., Ugrin, M., Nikčević, G., Suvajdžić-Vuković, N., Tomin, D., Vidović, A., Virijević, M., Pavlović, S.,& Colović, M.. (2012). Prognostic Impact of NPM1 Mutations in Serbian Adult Patients with Acute Myeloid Leukemia. in Acta Haematologica
Karger, Basel., 128(4), 203-212.
https://doi.org/10.1159/000339506

Kuzmanović M, Tošić N, Čolović N, Karan-Đurašević T, Spasovski V, Ugrin M, Nikčević G, Suvajdžić-Vuković N, Tomin D, Vidović A, Virijević M, Pavlović S, Colović M. Prognostic Impact of NPM1 Mutations in Serbian Adult Patients with Acute Myeloid Leukemia. in Acta Haematologica. 2012;128(4):203-212.
doi:10.1159/000339506 .

Kuzmanović, Milos, Tošić, Nataša, Čolović, Nataša, Karan-Đurašević, Teodora, Spasovski, Vesna, Ugrin, Milena, Nikčević, Gordana, Suvajdžić-Vuković, Nada, Tomin, Dragica, Vidović, Ana, Virijević, Marijana, Pavlović, Sonja, Colović, Milica, "Prognostic Impact of NPM1 Mutations in Serbian Adult Patients with Acute Myeloid Leukemia" in Acta Haematologica, 128, no. 4 (2012):203-212,
https://doi.org/10.1159/000339506 . .

TY  - JOUR
AU  - Kotur, Nikola
AU  - Stanković, Biljana
AU  - Klaassen, Kristel
AU  - Georgitsi, Marianthi
AU  - Vicha, Anna
AU  - Leontari, Iliana
AU  - Dokmanović, Lidija
AU  - Janić, Dragana
AU  - Krstovski, Nada
AU  - Karan-Đurašević, Teodora
AU  - Ugrin, Milena
AU  - Stojiljković, Maja
AU  - Nikčević, Gordana
AU  - Simeonidis, Argiris
AU  - Sivolapenko, Gregory
AU  - Pavlović, Sonja
AU  - Partinos, George P.
AU  - Zukić, Branka
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/554
AB  - Aim: TPMT activity is characterized by a trimodal distribution, namely low, intermediate and high methylator. TPMT gene promoter contains a variable number of GC-rich tandem repeats (VNTRs), namely A, B and C, ranging from three to nine repeats in length in an A(n)B(m)C architecture. We have previously shown that the VNTR architecture in the TPMT gene promoter affects TPMT gene transcription. Materials, methods & results: Here we demonstrate, using reporter assays, that 6-mercaptopurine (6-MP) treatment results in a VNTR architecture-dependent decrease of TPMT gene transcription, mediated by the binding of newly recruited protein complexes to the TPMT gene promoter, upon 6-MP treatment. We also show that acute lymphoblastic leukemia patients undergoing 6-MP treatment display a VNTR architecture-dependent response to 6-MP. Conclusion: These data suggest that the TPMT gene promoter VNTR architecture can be potentially used as a pharmacogenomic marker to predict toxicity due to 6-MP treatment in acute lymphoblastic leukemia patients.
PB  - Future Medicine Ltd, London
T2  - Pharmacogenomics
T1  - 6-mercaptopurine influences TPMT gene transcription in a TPMT gene promoter variable number of tandem repeats-dependent manner
EP  - 295
IS  - 3
SP  - 283
VL  - 13
DO  - 10.2217/PGS.11.153
ER  - 

@article{
author = "Kotur, Nikola and Stanković, Biljana and Klaassen, Kristel and Georgitsi, Marianthi and Vicha, Anna and Leontari, Iliana and Dokmanović, Lidija and Janić, Dragana and Krstovski, Nada and Karan-Đurašević, Teodora and Ugrin, Milena and Stojiljković, Maja and Nikčević, Gordana and Simeonidis, Argiris and Sivolapenko, Gregory and Pavlović, Sonja and Partinos, George P. and Zukić, Branka",
year = "2012",
abstract = "Aim: TPMT activity is characterized by a trimodal distribution, namely low, intermediate and high methylator. TPMT gene promoter contains a variable number of GC-rich tandem repeats (VNTRs), namely A, B and C, ranging from three to nine repeats in length in an A(n)B(m)C architecture. We have previously shown that the VNTR architecture in the TPMT gene promoter affects TPMT gene transcription. Materials, methods & results: Here we demonstrate, using reporter assays, that 6-mercaptopurine (6-MP) treatment results in a VNTR architecture-dependent decrease of TPMT gene transcription, mediated by the binding of newly recruited protein complexes to the TPMT gene promoter, upon 6-MP treatment. We also show that acute lymphoblastic leukemia patients undergoing 6-MP treatment display a VNTR architecture-dependent response to 6-MP. Conclusion: These data suggest that the TPMT gene promoter VNTR architecture can be potentially used as a pharmacogenomic marker to predict toxicity due to 6-MP treatment in acute lymphoblastic leukemia patients.",
publisher = "Future Medicine Ltd, London",
journal = "Pharmacogenomics",
title = "6-mercaptopurine influences TPMT gene transcription in a TPMT gene promoter variable number of tandem repeats-dependent manner",
pages = "295-283",
number = "3",
volume = "13",
doi = "10.2217/PGS.11.153"
}

Kotur, N., Stanković, B., Klaassen, K., Georgitsi, M., Vicha, A., Leontari, I., Dokmanović, L., Janić, D., Krstovski, N., Karan-Đurašević, T., Ugrin, M., Stojiljković, M., Nikčević, G., Simeonidis, A., Sivolapenko, G., Pavlović, S., Partinos, G. P.,& Zukić, B.. (2012). 6-mercaptopurine influences TPMT gene transcription in a TPMT gene promoter variable number of tandem repeats-dependent manner. in Pharmacogenomics
Future Medicine Ltd, London., 13(3), 283-295.
https://doi.org/10.2217/PGS.11.153

Kotur N, Stanković B, Klaassen K, Georgitsi M, Vicha A, Leontari I, Dokmanović L, Janić D, Krstovski N, Karan-Đurašević T, Ugrin M, Stojiljković M, Nikčević G, Simeonidis A, Sivolapenko G, Pavlović S, Partinos GP, Zukić B. 6-mercaptopurine influences TPMT gene transcription in a TPMT gene promoter variable number of tandem repeats-dependent manner. in Pharmacogenomics. 2012;13(3):283-295.
doi:10.2217/PGS.11.153 .

Kotur, Nikola, Stanković, Biljana, Klaassen, Kristel, Georgitsi, Marianthi, Vicha, Anna, Leontari, Iliana, Dokmanović, Lidija, Janić, Dragana, Krstovski, Nada, Karan-Đurašević, Teodora, Ugrin, Milena, Stojiljković, Maja, Nikčević, Gordana, Simeonidis, Argiris, Sivolapenko, Gregory, Pavlović, Sonja, Partinos, George P., Zukić, Branka, "6-mercaptopurine influences TPMT gene transcription in a TPMT gene promoter variable number of tandem repeats-dependent manner" in Pharmacogenomics, 13, no. 3 (2012):283-295,
https://doi.org/10.2217/PGS.11.153 . .

TY  - CONF
AU  - Karan-Đurašević, Teodora
AU  - Palibrk, V.
AU  - Tošić, Nataša
AU  - Kostić, Tatjana 
AU  - Spasovski, Vesna
AU  - Nikčević, Gordana
AU  - Srzentić Dražilov, Sanja
AU  - Glumac, Irena
AU  - Colović, M.
AU  - Čolović, Nataša
AU  - Antić, Darko
AU  - Mihaljević, B.
AU  - Scorilas, A.
AU  - Kontos, C.
AU  - Pavlović, Sonja
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/613
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica-The Hematology Journal
T1  - Expression of bcl2l12 gene in chronic lymphocytic leukemia: association with clinical and molecular prognostic markers
EP  - 298
SP  - 298
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_imagine_613
ER  - 

@conference{
author = "Karan-Đurašević, Teodora and Palibrk, V. and Tošić, Nataša and Kostić, Tatjana  and Spasovski, Vesna and Nikčević, Gordana and Srzentić Dražilov, Sanja and Glumac, Irena and Colović, M. and Čolović, Nataša and Antić, Darko and Mihaljević, B. and Scorilas, A. and Kontos, C. and Pavlović, Sonja",
year = "2012",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica-The Hematology Journal",
title = "Expression of bcl2l12 gene in chronic lymphocytic leukemia: association with clinical and molecular prognostic markers",
pages = "298-298",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_imagine_613"
}

Karan-Đurašević, T., Palibrk, V., Tošić, N., Kostić, T., Spasovski, V., Nikčević, G., Srzentić Dražilov, S., Glumac, I., Colović, M., Čolović, N., Antić, D., Mihaljević, B., Scorilas, A., Kontos, C.,& Pavlović, S.. (2012). Expression of bcl2l12 gene in chronic lymphocytic leukemia: association with clinical and molecular prognostic markers. in Haematologica-The Hematology Journal
Ferrata Storti Foundation, Pavia., 97, 298-298.
https://hdl.handle.net/21.15107/rcub_imagine_613

Karan-Đurašević T, Palibrk V, Tošić N, Kostić T, Spasovski V, Nikčević G, Srzentić Dražilov S, Glumac I, Colović M, Čolović N, Antić D, Mihaljević B, Scorilas A, Kontos C, Pavlović S. Expression of bcl2l12 gene in chronic lymphocytic leukemia: association with clinical and molecular prognostic markers. in Haematologica-The Hematology Journal. 2012;97:298-298.
https://hdl.handle.net/21.15107/rcub_imagine_613 .

Karan-Đurašević, Teodora, Palibrk, V., Tošić, Nataša, Kostić, Tatjana , Spasovski, Vesna, Nikčević, Gordana, Srzentić Dražilov, Sanja, Glumac, Irena, Colović, M., Čolović, Nataša, Antić, Darko, Mihaljević, B., Scorilas, A., Kontos, C., Pavlović, Sonja, "Expression of bcl2l12 gene in chronic lymphocytic leukemia: association with clinical and molecular prognostic markers" in Haematologica-The Hematology Journal, 97 (2012):298-298,
https://hdl.handle.net/21.15107/rcub_imagine_613 .

TY  - CONF
AU  - Spasovski, Vesna
AU  - Tošić, Nataša
AU  - Nikčević, Gordana
AU  - Stojiljković, Maja
AU  - Zukić, Branka
AU  - Radmilović, Milena
AU  - Karan-Đurašević, Teodora
AU  - Srzentić Dražilov, Sanja
AU  - Čolović, Nataša
AU  - Colović, M.
AU  - Pavlović, Sonja
PY  - 2012
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/603
PB  - Ferrata Storti Foundation, Pavia
C3  - Haematologica-The Hematology Journal
T1  - The 46/1 haplotype is involved in transcriptional regulation of janus kinase 2 gene
EP  - 373
SP  - 372
VL  - 97
UR  - https://hdl.handle.net/21.15107/rcub_imagine_603
ER  - 

@conference{
author = "Spasovski, Vesna and Tošić, Nataša and Nikčević, Gordana and Stojiljković, Maja and Zukić, Branka and Radmilović, Milena and Karan-Đurašević, Teodora and Srzentić Dražilov, Sanja and Čolović, Nataša and Colović, M. and Pavlović, Sonja",
year = "2012",
publisher = "Ferrata Storti Foundation, Pavia",
journal = "Haematologica-The Hematology Journal",
title = "The 46/1 haplotype is involved in transcriptional regulation of janus kinase 2 gene",
pages = "373-372",
volume = "97",
url = "https://hdl.handle.net/21.15107/rcub_imagine_603"
}

Spasovski, V., Tošić, N., Nikčević, G., Stojiljković, M., Zukić, B., Radmilović, M., Karan-Đurašević, T., Srzentić Dražilov, S., Čolović, N., Colović, M.,& Pavlović, S.. (2012). The 46/1 haplotype is involved in transcriptional regulation of janus kinase 2 gene. in Haematologica-The Hematology Journal
Ferrata Storti Foundation, Pavia., 97, 372-373.
https://hdl.handle.net/21.15107/rcub_imagine_603

Spasovski V, Tošić N, Nikčević G, Stojiljković M, Zukić B, Radmilović M, Karan-Đurašević T, Srzentić Dražilov S, Čolović N, Colović M, Pavlović S. The 46/1 haplotype is involved in transcriptional regulation of janus kinase 2 gene. in Haematologica-The Hematology Journal. 2012;97:372-373.
https://hdl.handle.net/21.15107/rcub_imagine_603 .

Spasovski, Vesna, Tošić, Nataša, Nikčević, Gordana, Stojiljković, Maja, Zukić, Branka, Radmilović, Milena, Karan-Đurašević, Teodora, Srzentić Dražilov, Sanja, Čolović, Nataša, Colović, M., Pavlović, Sonja, "The 46/1 haplotype is involved in transcriptional regulation of janus kinase 2 gene" in Haematologica-The Hematology Journal, 97 (2012):372-373,
https://hdl.handle.net/21.15107/rcub_imagine_603 .

TY  - JOUR
AU  - Nikčević, Gordana
AU  - Kovačević Grujičić, Nataša
AU  - Mojsin, Marija
AU  - Krstić, A.
AU  - Savić, T.
AU  - Stevanović, Milena
PY  - 2011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/485
AB  - Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates. Despite the mounting evidence that Sox3/SOX3 is one of the key players in the development of the nervous system, limited data are available regarding the transcriptional regulation of its expression. This review is focused on the retinoic acid induced regulation of SOX3 gene expression, with particular emphasis on the involvement of retinoid receptors. Experiments with human embryonal carcinoma cells identified two response elements involved in retinoic acid/retinoid X receptor-dependent activation of the SOX3 gene expression: distal atypical retinoic acid-response element, consisting of two unique G-rich boxes separated by 49 bp, and proximal element comprising DR-3-like motif, composed of two imperfect hexameric half-sites. Importantly, the retinoic acid-induced SOX3 gene expression could be significantly down-regulated by a synthetic antagonist of retinoid receptors. This cell model provides a solid base for further studies on mechanism(s) underlying regulation of expression of SOX3 gene, which could improve the understanding of molecular signals that induce neurogenesis in the stem/progenitor cells both during development and in adulthood.
PB  - Acad Sciences Czech Republic, Inst Physiology, Prague 4
T2  - Physiological Research
T1  - Regulation of the SOX3 Gene Expression by Retinoid Receptors
EP  - S91
SP  - S83
VL  - 60
DO  - 10.33549/physiolres.932184
ER  - 

@article{
author = "Nikčević, Gordana and Kovačević Grujičić, Nataša and Mojsin, Marija and Krstić, A. and Savić, T. and Stevanović, Milena",
year = "2011",
abstract = "Sox3/SOX3 gene is considered to be one of the earliest neural markers in vertebrates. Despite the mounting evidence that Sox3/SOX3 is one of the key players in the development of the nervous system, limited data are available regarding the transcriptional regulation of its expression. This review is focused on the retinoic acid induced regulation of SOX3 gene expression, with particular emphasis on the involvement of retinoid receptors. Experiments with human embryonal carcinoma cells identified two response elements involved in retinoic acid/retinoid X receptor-dependent activation of the SOX3 gene expression: distal atypical retinoic acid-response element, consisting of two unique G-rich boxes separated by 49 bp, and proximal element comprising DR-3-like motif, composed of two imperfect hexameric half-sites. Importantly, the retinoic acid-induced SOX3 gene expression could be significantly down-regulated by a synthetic antagonist of retinoid receptors. This cell model provides a solid base for further studies on mechanism(s) underlying regulation of expression of SOX3 gene, which could improve the understanding of molecular signals that induce neurogenesis in the stem/progenitor cells both during development and in adulthood.",
publisher = "Acad Sciences Czech Republic, Inst Physiology, Prague 4",
journal = "Physiological Research",
title = "Regulation of the SOX3 Gene Expression by Retinoid Receptors",
pages = "S91-S83",
volume = "60",
doi = "10.33549/physiolres.932184"
}

Nikčević, G., Kovačević Grujičić, N., Mojsin, M., Krstić, A., Savić, T.,& Stevanović, M.. (2011). Regulation of the SOX3 Gene Expression by Retinoid Receptors. in Physiological Research
Acad Sciences Czech Republic, Inst Physiology, Prague 4., 60, S83-S91.
https://doi.org/10.33549/physiolres.932184

Nikčević G, Kovačević Grujičić N, Mojsin M, Krstić A, Savić T, Stevanović M. Regulation of the SOX3 Gene Expression by Retinoid Receptors. in Physiological Research. 2011;60:S83-S91.
doi:10.33549/physiolres.932184 .

Nikčević, Gordana, Kovačević Grujičić, Nataša, Mojsin, Marija, Krstić, A., Savić, T., Stevanović, Milena, "Regulation of the SOX3 Gene Expression by Retinoid Receptors" in Physiological Research, 60 (2011):S83-S91,
https://doi.org/10.33549/physiolres.932184 . .

TY  - JOUR
AU  - Milivojević, Milena
AU  - Nikčević, Gordana
AU  - Kovačević Grujičić, Nataša
AU  - Krstić, A.
AU  - Mojsin, Marija
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
PY  - 2010
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/448
AB  - SOX2 transkripcioni faktor ima ključnu ulogu u procesima embrionalnog razvića i predstavlja univerzalni marker pluripotentnih matičnih ćelija. S obzirom na funkcionalnu redundantnost i preklapajući profil ekspresije članova SOXB1 podgrupe tokom razvića, cilj ovog rada bio je da ispita potencijalne zajedničke aspekte regulacije ekspresije SOX2 i SOX3 gena. Naime, ispitivan je uticaj odabranih transkripcionih faktora na regulaciju ekspresije SOX2 gena u NT2/D1 ćelijskoj liniji. Analizirani su oni faktori za koje je prethodno pokazano da su uključeni u modulaciju aktivnosti humanog SOX3 gena. Rezultati ovih istraživanja ukazuju da opšti transkripcioni faktori (NF-Y, Sp1 i MAZ), članovi TALE familije proteina (Pbx1 i Meis1), kao i retinoičnom kiselinom aktiviran nuklearni receptor RXRα dovode do povećane ekspresije SOX2 proteina. Ispitivanje transkripcionih faktora uključenih u regulaciju ekspresije SOX gena je značajno za bolje razumevanje signalnih puteva koji su aktivni u pluripotentnim matičnim ćelijama.
AB  - SOX2 is a key transcription factor in embryonic development representing a universal marker of pluripotent stem cells. Based on the functional redundancy and overlapping expression patterns of SOXB1 subgroup members during development, the goal of this study has been to analyze if some aspects of regulation of expression are preserved between human SOX2 and SOX3 genes. Thus, we have tested several transcription factors previously demonstrated to play roles in controlling SOX3 gene activity for potential participation in the regulation of SOX2 gene expression in NT2/D1 cells. Here we report on the activation of SOX2 expression by ubiquitous transcription factors (NF-Y, Sp1 and MAZ), TALE family members (Pbx1 and Meis1), as well as liganded RXRα. Elucidating components involved in the regulation of SOX gene expression represent a valuable contribution in unraveling the regulatory networks operating in pluripotent embryonic cells.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji
T1  - Involvement of ubiquitous and tale transcription factors, as well as liganded RXRα, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line
EP  - 210
IS  - 2
SP  - 199
VL  - 62
DO  - 10.2298/ABS1002199M
ER  - 

@article{
author = "Milivojević, Milena and Nikčević, Gordana and Kovačević Grujičić, Nataša and Krstić, A. and Mojsin, Marija and Drakulić, Danijela and Stevanović, Milena",
year = "2010",
abstract = "SOX2 transkripcioni faktor ima ključnu ulogu u procesima embrionalnog razvića i predstavlja univerzalni marker pluripotentnih matičnih ćelija. S obzirom na funkcionalnu redundantnost i preklapajući profil ekspresije članova SOXB1 podgrupe tokom razvića, cilj ovog rada bio je da ispita potencijalne zajedničke aspekte regulacije ekspresije SOX2 i SOX3 gena. Naime, ispitivan je uticaj odabranih transkripcionih faktora na regulaciju ekspresije SOX2 gena u NT2/D1 ćelijskoj liniji. Analizirani su oni faktori za koje je prethodno pokazano da su uključeni u modulaciju aktivnosti humanog SOX3 gena. Rezultati ovih istraživanja ukazuju da opšti transkripcioni faktori (NF-Y, Sp1 i MAZ), članovi TALE familije proteina (Pbx1 i Meis1), kao i retinoičnom kiselinom aktiviran nuklearni receptor RXRα dovode do povećane ekspresije SOX2 proteina. Ispitivanje transkripcionih faktora uključenih u regulaciju ekspresije SOX gena je značajno za bolje razumevanje signalnih puteva koji su aktivni u pluripotentnim matičnim ćelijama., SOX2 is a key transcription factor in embryonic development representing a universal marker of pluripotent stem cells. Based on the functional redundancy and overlapping expression patterns of SOXB1 subgroup members during development, the goal of this study has been to analyze if some aspects of regulation of expression are preserved between human SOX2 and SOX3 genes. Thus, we have tested several transcription factors previously demonstrated to play roles in controlling SOX3 gene activity for potential participation in the regulation of SOX2 gene expression in NT2/D1 cells. Here we report on the activation of SOX2 expression by ubiquitous transcription factors (NF-Y, Sp1 and MAZ), TALE family members (Pbx1 and Meis1), as well as liganded RXRα. Elucidating components involved in the regulation of SOX gene expression represent a valuable contribution in unraveling the regulatory networks operating in pluripotent embryonic cells.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji, Involvement of ubiquitous and tale transcription factors, as well as liganded RXRα, in the regulation of human SOX2 gene expression in the NT2/D1 embryonal carcinoma cell line",
pages = "210-199",
number = "2",
volume = "62",
doi = "10.2298/ABS1002199M"
}

Milivojević, M., Nikčević, G., Kovačević Grujičić, N., Krstić, A., Mojsin, M., Drakulić, D.,& Stevanović, M.. (2010). Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(2), 199-210.
https://doi.org/10.2298/ABS1002199M

Milivojević M, Nikčević G, Kovačević Grujičić N, Krstić A, Mojsin M, Drakulić D, Stevanović M. Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji. in Archives of Biological Sciences. 2010;62(2):199-210.
doi:10.2298/ABS1002199M .

Milivojević, Milena, Nikčević, Gordana, Kovačević Grujičić, Nataša, Krstić, A., Mojsin, Marija, Drakulić, Danijela, Stevanović, Milena, "Uticaj opštih transkripcionih faktora, tale proteina i ligandom aktiviranog nuklearnog receptora RXRα na regulaciju ekspresije humanog SOX2 gena u embrionalnoj karcinomskoj NT2/D1 ćelijskoj liniji" in Archives of Biological Sciences, 62, no. 2 (2010):199-210,
https://doi.org/10.2298/ABS1002199M . .