TY  - CONF
AU  - Ćurčić, Jovana
AU  - Malešević, Milka
AU  - Jovčić, Branko
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1308
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2359
AB  - Biofilm-associated infections are the main cause of biomaterial implant failure today. The increasing prevalence of antibiotic-resistant pathogens often results in the only solution of implant movement, with serious consequences for patients. Recently, various antimicrobial agents have been recognized as a promising strategy to prevent biofilm formation on implant surfaces. Quorum sensing (QS) plays a central role in the control of bacterial virulence and biofilm formation. The use of quorum quenching (QQ) enzymes to target QS is therefore a promising innovative approach for the development of enzyme-based antivirulence therapeutics, which represent a potential solution to combat infections caused by multidrug-resistant pathogens. This study aimed to characterize the novel YtnP lactonase from the clinical isolate Stenotrophomonas maltophilia 6960 and to investigate its potential to combat the virulence of multidrug-resistant (MDR) Pseudomonas aeruginosa MMA83.
C3  - Hemijska industrija (Chemical Industry)
T1  - A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms
EP  - 61
IS  - 1S
SP  - 61
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2359
ER  - 

@conference{
author = "Ćurčić, Jovana and Malešević, Milka and Jovčić, Branko",
year = "2024",
abstract = "Biofilm-associated infections are the main cause of biomaterial implant failure today. The increasing prevalence of antibiotic-resistant pathogens often results in the only solution of implant movement, with serious consequences for patients. Recently, various antimicrobial agents have been recognized as a promising strategy to prevent biofilm formation on implant surfaces. Quorum sensing (QS) plays a central role in the control of bacterial virulence and biofilm formation. The use of quorum quenching (QQ) enzymes to target QS is therefore a promising innovative approach for the development of enzyme-based antivirulence therapeutics, which represent a potential solution to combat infections caused by multidrug-resistant pathogens. This study aimed to characterize the novel YtnP lactonase from the clinical isolate Stenotrophomonas maltophilia 6960 and to investigate its potential to combat the virulence of multidrug-resistant (MDR) Pseudomonas aeruginosa MMA83.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms",
pages = "61-61",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2359"
}

Ćurčić, J., Malešević, M.,& Jovčić, B.. (2024). A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms. in Hemijska industrija (Chemical Industry), 78(1S), 61-61.
https://hdl.handle.net/21.15107/rcub_imagine_2359

Ćurčić J, Malešević M, Jovčić B. A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms. in Hemijska industrija (Chemical Industry). 2024;78(1S):61-61.
https://hdl.handle.net/21.15107/rcub_imagine_2359 .

Ćurčić, Jovana, Malešević, Milka, Jovčić, Branko, "A novel thermostable YtnP lactonase inhibits biofilm formation and induces decomposition of preformed Pseudomonas aeruginosa biofilms" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):61-61,
https://hdl.handle.net/21.15107/rcub_imagine_2359 .

TY  - JOUR
AU  - Ćurčić, Jovana
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Malešević, Milka
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0141813024012248
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2326
AB  - Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.
PB  - Elsevier
T2  - International Journal of Biological Macromolecules
T2  - International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules
T1  - A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo
SP  - 130421
DO  - 10.1016/j.ijbiomac.2024.130421
ER  - 

@article{
author = "Ćurčić, Jovana and Dinić, Miroslav and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Malešević, Milka",
year = "2024",
abstract = "Infections caused by multidrug-resistant pathogens are one of the biggest challenges facing the healthcare system today. Quorum quenching (QQ) enzymes have the potential to be used as innovative enzyme-based antivirulence therapeutics to combat infections caused by multidrug-resistant pathogens. The main objective of this research was to describe the novel YtnP lactonase derived from the clinical isolate Stenotrophomonas maltophilia and to investigate its antivirulence potential against multidrug-resistant Pseudomonas aeruginosa MMA83. YtnP lactonase, the QQ enzyme, belongs to the family of metallo-β-lactamases. The recombinant enzyme has several advantageous biotechnological properties, such as high thermostability, activity in a wide pH range, and no cytotoxic effect. High-performance liquid chromatography analysis revealed the activity of recombinant YtnP lactonase toward a wide range of N-acyl-homoserine lactones (AHLs), quorum sensing signaling molecules, with a higher preference for long-chain AHLs. Recombinant YtnP lactonase was shown to inhibit P. aeruginosa MMA83 biofilm formation, induce biofilm decomposition, and reduce extracellular virulence factors production. Moreover, the lifespan of MMA83-infected Caenorhabditis elegans was prolonged with YtnP lactonase treatment. YtnP lactonase showed synergistic inhibitory activity in combination with gentamicin and acted additively with meropenem against MMA83. The described properties make YtnP lactonase a promising therapeutic candidate for the development of next-generation antivirulence agents.",
publisher = "Elsevier",
journal = "International Journal of Biological Macromolecules, International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules",
title = "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo",
pages = "130421",
doi = "10.1016/j.ijbiomac.2024.130421"
}

Ćurčić, J., Dinić, M., Novović, K., Vasiljević, Z., Kojić, M., Jovčić, B.,& Malešević, M.. (2024). A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules
Elsevier., 130421.
https://doi.org/10.1016/j.ijbiomac.2024.130421

Ćurčić J, Dinić M, Novović K, Vasiljević Z, Kojić M, Jovčić B, Malešević M. A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo. in International Journal of Biological Macromolecules. 2024;:130421.
doi:10.1016/j.ijbiomac.2024.130421 .

Ćurčić, Jovana, Dinić, Miroslav, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Malešević, Milka, "A novel thermostable YtnP lactonase from Stenotrophomonas maltophilia inhibits Pseudomonas aeruginosa virulence in vitro and in vivo" in International Journal of Biological Macromolecules (2024):130421,
https://doi.org/10.1016/j.ijbiomac.2024.130421 . .

TY  - CONF
AU  - Novović, Katarina
AU  - Radovanović, Milica
AU  - Milić, Vukašin
AU  - Gajić, Ina
AU  - Vasiljević, Zorica
AU  - Jovčić, Branko
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2134
AB  - Introduction: Multidrug-resistant (MDR) Acinetobacter baumannii has been recognized as one of the
most serious healthcare challenges worldwide. Although tigecycline represents one of the last resort
therapies for MDR A. baumannii, resistance to this antibiotic has been reported and mostly is mediated
by AdeABC efflux pump. The aim of our study was to investigate the molecular mechanism responsible
for tigecycline resistance of thirty-seven A. baumannii isolates from Balkan medical settings (Serbia,
Bosnia and Herzegovina and Montenegro) gathered in 2016 and 2022.
Methods: Minimal inhibitory concentration (MIC) values for tigecycline were determined using microdilution method according to EUCAST guidelines. Inhibition of the efflux of tigecycline wastested by
the same method using a combination of antibiotic and efflux pump inhibitor (CCCP). Amino acid alternations within AdeS and AdeR proteins were detected by comparing to sequences of referent isolates
ATCC19606 and ATCC17978. Expression of the adeB gene ofselected isolates was monitored by RT-qPCR.
Results: All tested isolates were resistant to tigecycline and showed significant decrease in tigecycline
MIC values in presence of CCCP (≥16-fold reduction) indicating that antibiotic efflux is responsible for
tigecycline resistance. The analysis of two-component system AdeRS, regulatory system of RND efflux
pump AdeABC, revealed that most of the isolates have G186V and N268H alternations in AdeS (n=32),
while most common changes in AdeR were V120I and A136V (n=29). In addition, RT-qPCR showed that
selected isolates upregulate expression of the adeB gene (from 1,13- to 3-fold).
Conclusion: This study revealed that AdeABC overexpression is the main mechanism of tigecycline resistance in A. baumannii isolated in Balkan hospitals.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - AdeABC efflux pump-mediated resistance to tigecycline in Acinetobacter baumannii isolates from Balkan hospitals
EP  - 154
SP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2134
ER  - 

@conference{
author = "Novović, Katarina and Radovanović, Milica and Milić, Vukašin and Gajić, Ina and Vasiljević, Zorica and Jovčić, Branko",
year = "2023",
abstract = "Introduction: Multidrug-resistant (MDR) Acinetobacter baumannii has been recognized as one of the
most serious healthcare challenges worldwide. Although tigecycline represents one of the last resort
therapies for MDR A. baumannii, resistance to this antibiotic has been reported and mostly is mediated
by AdeABC efflux pump. The aim of our study was to investigate the molecular mechanism responsible
for tigecycline resistance of thirty-seven A. baumannii isolates from Balkan medical settings (Serbia,
Bosnia and Herzegovina and Montenegro) gathered in 2016 and 2022.
Methods: Minimal inhibitory concentration (MIC) values for tigecycline were determined using microdilution method according to EUCAST guidelines. Inhibition of the efflux of tigecycline wastested by
the same method using a combination of antibiotic and efflux pump inhibitor (CCCP). Amino acid alternations within AdeS and AdeR proteins were detected by comparing to sequences of referent isolates
ATCC19606 and ATCC17978. Expression of the adeB gene ofselected isolates was monitored by RT-qPCR.
Results: All tested isolates were resistant to tigecycline and showed significant decrease in tigecycline
MIC values in presence of CCCP (≥16-fold reduction) indicating that antibiotic efflux is responsible for
tigecycline resistance. The analysis of two-component system AdeRS, regulatory system of RND efflux
pump AdeABC, revealed that most of the isolates have G186V and N268H alternations in AdeS (n=32),
while most common changes in AdeR were V120I and A136V (n=29). In addition, RT-qPCR showed that
selected isolates upregulate expression of the adeB gene (from 1,13- to 3-fold).
Conclusion: This study revealed that AdeABC overexpression is the main mechanism of tigecycline resistance in A. baumannii isolated in Balkan hospitals.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "AdeABC efflux pump-mediated resistance to tigecycline in Acinetobacter baumannii isolates from Balkan hospitals",
pages = "154-154",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2134"
}

Novović, K., Radovanović, M., Milić, V., Gajić, I., Vasiljević, Z.,& Jovčić, B.. (2023). AdeABC efflux pump-mediated resistance to tigecycline in Acinetobacter baumannii isolates from Balkan hospitals. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 154-154.
https://hdl.handle.net/21.15107/rcub_imagine_2134

Novović K, Radovanović M, Milić V, Gajić I, Vasiljević Z, Jovčić B. AdeABC efflux pump-mediated resistance to tigecycline in Acinetobacter baumannii isolates from Balkan hospitals. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:154-154.
https://hdl.handle.net/21.15107/rcub_imagine_2134 .

Novović, Katarina, Radovanović, Milica, Milić, Vukašin, Gajić, Ina, Vasiljević, Zorica, Jovčić, Branko, "AdeABC efflux pump-mediated resistance to tigecycline in Acinetobacter baumannii isolates from Balkan hospitals" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):154-154,
https://hdl.handle.net/21.15107/rcub_imagine_2134 .

TY  - JOUR
AU  - Malešević, Milka
AU  - Gardijan, Lazar
AU  - Miljković, Marija
AU  - O'Connor, Paula M
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Cotter, Paul D
AU  - Jovanovic, Goran
AU  - Kojić, Milan
PY  - 2023
UR  - https://doi.org/10.1093/lambio/ovad004
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1828
AB  - Lactic acid bacterium Lactococcus lactis BGBU1-4 produces 43 amino acids (aa) long bacteriocin, lactolisterin BU (LBU), a 5.161 kDa peptide with potent antibacterial activity against many Gram-positive pathogens. In addition, BGBU1-4 produces an additional unknown product of 3.642 kDa with antibacterial activity. Here, we determined that the significant amount of naturally produced LBU breaks down to create a 3.642 kDa truncated form of LBU bacteriocin consisting of 31 N-terminal aa (LBU1-31) that exhibits 12.5% the antibacterial activity of the full-length LBU. We showed that chemically synthesized LBU is stable and 50% less active than native LBU, and so we used the synthetic peptides of LBU and its variants to further study their activities and antibacterial potential. Deletion analysis of LBU revealed that the 24 N-terminal aa of LBU (LBU1-24) are responsible for antibacterial activity, while downstream aa (25–43) determine the species-specific effectiveness of LBU. Although LBU1-31 contains aa 1–24, the truncation at position 31 is predicted to change the structure within aa 15–31 and might impact on antibacterial activity. Intriguingly, whole genome sequencing and genome mining established that BGBU1-4 is abundant in genes that encode potential antibacterials, but produces LBU and its breakdown product LBU1-31 exclusively.
T2  - Letters in Applied Microbiology
T2  - Letters in Applied MicrobiologyLetters in Applied Microbiology
T1  - Exploring the antibacterial potential of Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 by genome mining, bacteriocin gene overexpression, and chemical protein synthesis of lactolisterin BU variants
IS  - 2
SP  - ovad004
VL  - 76
DO  - 10.1093/lambio/ovad004
ER  - 

@article{
author = "Malešević, Milka and Gardijan, Lazar and Miljković, Marija and O'Connor, Paula M and Mirković, Nemanja and Jovčić, Branko and Cotter, Paul D and Jovanovic, Goran and Kojić, Milan",
year = "2023",
abstract = "Lactic acid bacterium Lactococcus lactis BGBU1-4 produces 43 amino acids (aa) long bacteriocin, lactolisterin BU (LBU), a 5.161 kDa peptide with potent antibacterial activity against many Gram-positive pathogens. In addition, BGBU1-4 produces an additional unknown product of 3.642 kDa with antibacterial activity. Here, we determined that the significant amount of naturally produced LBU breaks down to create a 3.642 kDa truncated form of LBU bacteriocin consisting of 31 N-terminal aa (LBU1-31) that exhibits 12.5% the antibacterial activity of the full-length LBU. We showed that chemically synthesized LBU is stable and 50% less active than native LBU, and so we used the synthetic peptides of LBU and its variants to further study their activities and antibacterial potential. Deletion analysis of LBU revealed that the 24 N-terminal aa of LBU (LBU1-24) are responsible for antibacterial activity, while downstream aa (25–43) determine the species-specific effectiveness of LBU. Although LBU1-31 contains aa 1–24, the truncation at position 31 is predicted to change the structure within aa 15–31 and might impact on antibacterial activity. Intriguingly, whole genome sequencing and genome mining established that BGBU1-4 is abundant in genes that encode potential antibacterials, but produces LBU and its breakdown product LBU1-31 exclusively.",
journal = "Letters in Applied Microbiology, Letters in Applied MicrobiologyLetters in Applied Microbiology",
title = "Exploring the antibacterial potential of Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 by genome mining, bacteriocin gene overexpression, and chemical protein synthesis of lactolisterin BU variants",
number = "2",
pages = "ovad004",
volume = "76",
doi = "10.1093/lambio/ovad004"
}

Malešević, M., Gardijan, L., Miljković, M., O'Connor, P. M., Mirković, N., Jovčić, B., Cotter, P. D., Jovanovic, G.,& Kojić, M.. (2023). Exploring the antibacterial potential of Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 by genome mining, bacteriocin gene overexpression, and chemical protein synthesis of lactolisterin BU variants. in Letters in Applied Microbiology, 76(2), ovad004.
https://doi.org/10.1093/lambio/ovad004

Malešević M, Gardijan L, Miljković M, O'Connor PM, Mirković N, Jovčić B, Cotter PD, Jovanovic G, Kojić M. Exploring the antibacterial potential of Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 by genome mining, bacteriocin gene overexpression, and chemical protein synthesis of lactolisterin BU variants. in Letters in Applied Microbiology. 2023;76(2):ovad004.
doi:10.1093/lambio/ovad004 .

Malešević, Milka, Gardijan, Lazar, Miljković, Marija, O'Connor, Paula M, Mirković, Nemanja, Jovčić, Branko, Cotter, Paul D, Jovanovic, Goran, Kojić, Milan, "Exploring the antibacterial potential of Lactococcus lactis subsp. lactis bv. diacetylactis BGBU1-4 by genome mining, bacteriocin gene overexpression, and chemical protein synthesis of lactolisterin BU variants" in Letters in Applied Microbiology, 76, no. 2 (2023):ovad004,
https://doi.org/10.1093/lambio/ovad004 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Jovčić, Branko
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1786
AB  - : Acinetobacter baumannii is recognized as a clinically significant pathogen causing a wide
spectrum of nosocomial infections. Colistin was considered a last-resort antibiotic for the treatment of
infections caused by multidrug-resistant A. baumannii. Since the reintroduction of colistin, a number
of mechanisms of colistin resistance in A. baumannii have been reported, including complete loss of
LPS by inactivation of the biosynthetic pathway, modifications of target LPS driven by the addition
of phosphoethanolamine (PEtN) moieties to lipid A mediated by the chromosomal pmrCAB operon
and eptA gene-encoded enzymes or plasmid-encoded mcr genes and efflux of colistin from the cell.
In addition to resistance to colistin, widespread heteroresistance is another feature of A. baumannii
that leads to colistin treatment failure. This review aims to present a critical assessment of relevant
published (>50 experimental papers) up-to-date knowledge on the molecular mechanisms of colistin
resistance in A. baumannii with a detailed review of implicated mutations and the global distribution
of colistin-resistant strains.
PB  - MDPI
T2  - Antibiotics
T1  - Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology
IS  - 3
SP  - 516
VL  - 12
DO  - 10.3390/antibiotics12030516
ER  - 

@article{
author = "Novović, Katarina and Jovčić, Branko",
year = "2023",
abstract = ": Acinetobacter baumannii is recognized as a clinically significant pathogen causing a wide
spectrum of nosocomial infections. Colistin was considered a last-resort antibiotic for the treatment of
infections caused by multidrug-resistant A. baumannii. Since the reintroduction of colistin, a number
of mechanisms of colistin resistance in A. baumannii have been reported, including complete loss of
LPS by inactivation of the biosynthetic pathway, modifications of target LPS driven by the addition
of phosphoethanolamine (PEtN) moieties to lipid A mediated by the chromosomal pmrCAB operon
and eptA gene-encoded enzymes or plasmid-encoded mcr genes and efflux of colistin from the cell.
In addition to resistance to colistin, widespread heteroresistance is another feature of A. baumannii
that leads to colistin treatment failure. This review aims to present a critical assessment of relevant
published (>50 experimental papers) up-to-date knowledge on the molecular mechanisms of colistin
resistance in A. baumannii with a detailed review of implicated mutations and the global distribution
of colistin-resistant strains.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology",
number = "3",
pages = "516",
volume = "12",
doi = "10.3390/antibiotics12030516"
}

Novović, K.,& Jovčić, B.. (2023). Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology. in Antibiotics
MDPI., 12(3), 516.
https://doi.org/10.3390/antibiotics12030516

Novović K, Jovčić B. Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology. in Antibiotics. 2023;12(3):516.
doi:10.3390/antibiotics12030516 .

Novović, Katarina, Jovčić, Branko, "Colistin resistance in acinetobacter baumannii: Molecular mechanisms and epidemiology" in Antibiotics, 12, no. 3 (2023):516,
https://doi.org/10.3390/antibiotics12030516 . .

TY  - DATA
AU  - Kabić, Jovana
AU  - Novović, Katarina
AU  - Kekić, Dušan
AU  - Trudić, Anika
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Jovčić, Branko
AU  - Gajić, Ina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1785
T2  - Computational and Structural Biotechnology Journal
T2  - Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal
T1  - Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1785
ER  - 

@misc{
author = "Kabić, Jovana and Novović, Katarina and Kekić, Dušan and Trudić, Anika and Opavski, Nataša and Dimkić, Ivica and Jovčić, Branko and Gajić, Ina",
year = "2023",
journal = "Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal",
title = "Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1785"
}

Kabić, J., Novović, K., Kekić, D., Trudić, A., Opavski, N., Dimkić, I., Jovčić, B.,& Gajić, I.. (2023). Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045. in Computational and Structural Biotechnology Journal.
https://hdl.handle.net/21.15107/rcub_imagine_1785

Kabić J, Novović K, Kekić D, Trudić A, Opavski N, Dimkić I, Jovčić B, Gajić I. Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045. in Computational and Structural Biotechnology Journal. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1785 .

Kabić, Jovana, Novović, Katarina, Kekić, Dušan, Trudić, Anika, Opavski, Nataša, Dimkić, Ivica, Jovčić, Branko, Gajić, Ina, "Supplementary information for the article: Kabic, J., Novovic, K., Kekic, D., Trudic, A., Opavski, N., Dimkic, I., Jovcic, B., & Gajic, I. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. Computational and Structural Biotechnology Journal, 21, 574–585. https://doi.org/10.1016/j.csbj.2022.12.045" in Computational and Structural Biotechnology Journal (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1785 .

TY  - JOUR
AU  - Kabić, Jovana
AU  - Novović, Katarina
AU  - Kekić, Dušan
AU  - Trudić, Anika
AU  - Opavski, Nataša
AU  - Dimkić, Ivica
AU  - Jovčić, Branko
AU  - Gajić, Ina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S200103702200602X
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1784
AB  - This study aimed to investigate the prevalence and resistance mechanisms of colistin-resistant Acinetobacter baumannii (ColRAB) isolates in Serbia, assess their genetic relatedness to other circulating A. baumannii isolates in the neighbouring European countries, and analyse the global genomic epidemiology of ColRAB isolates. A total of 784 isolates of A. baumannii were recovered from hospitalised patients in Serbia between 2018 and 2021. The antimicrobial susceptibility testing was performed using disk diffusion and broth microdilution. All ColRAB isolates were subjected to DNA isolation and whole-genome sequencing (WGS). Overall, 3.94 % (n = 30) isolates were confirmed as ColRAB. Results of mutational and transcriptional analysis of genes associated with colistin resistance indicate the central role of the two-component regulating system, PmrAB, and increased expression of the pmrC gene in ColRAB. Most of the isolates (n = 29, 96.6 %) belonged to international clone II, with the most common sequence type being STPas2 (n = 23, 76.6 %). Based on the WGS analysis, ColRAB isolates belonging to the same ST isolated in various countries were grouped into the same clusters, indicating the global dissemination of several high-risk clonal lineages. Phylogenomic analysis of ColRAB isolates, together with all previously published A. baumannii genomes from South-Eastern European countries, showed that colistin resistance arose independently in several clonal lineages. Comparative genomic analysis revealed multiple genes with various roles (transcriptional regulation, transmembrane transport, outer membrane assembly, etc.), which might be associated with colistin resistance in A. baumannii. The obtained findings serve as the basis for further studies, contributing to a better understanding of colistin resistance mechanisms in A. baumannii.
T2  - Computational and Structural Biotechnology Journal
T2  - Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal
T1  - Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii
EP  - 585
SP  - 574
VL  - 21
DO  - 10.1016/j.csbj.2022.12.045
DO  - 10.1016/j.csbj.2022.12.045
ER  - 

@article{
author = "Kabić, Jovana and Novović, Katarina and Kekić, Dušan and Trudić, Anika and Opavski, Nataša and Dimkić, Ivica and Jovčić, Branko and Gajić, Ina",
year = "2023",
abstract = "This study aimed to investigate the prevalence and resistance mechanisms of colistin-resistant Acinetobacter baumannii (ColRAB) isolates in Serbia, assess their genetic relatedness to other circulating A. baumannii isolates in the neighbouring European countries, and analyse the global genomic epidemiology of ColRAB isolates. A total of 784 isolates of A. baumannii were recovered from hospitalised patients in Serbia between 2018 and 2021. The antimicrobial susceptibility testing was performed using disk diffusion and broth microdilution. All ColRAB isolates were subjected to DNA isolation and whole-genome sequencing (WGS). Overall, 3.94 % (n = 30) isolates were confirmed as ColRAB. Results of mutational and transcriptional analysis of genes associated with colistin resistance indicate the central role of the two-component regulating system, PmrAB, and increased expression of the pmrC gene in ColRAB. Most of the isolates (n = 29, 96.6 %) belonged to international clone II, with the most common sequence type being STPas2 (n = 23, 76.6 %). Based on the WGS analysis, ColRAB isolates belonging to the same ST isolated in various countries were grouped into the same clusters, indicating the global dissemination of several high-risk clonal lineages. Phylogenomic analysis of ColRAB isolates, together with all previously published A. baumannii genomes from South-Eastern European countries, showed that colistin resistance arose independently in several clonal lineages. Comparative genomic analysis revealed multiple genes with various roles (transcriptional regulation, transmembrane transport, outer membrane assembly, etc.), which might be associated with colistin resistance in A. baumannii. The obtained findings serve as the basis for further studies, contributing to a better understanding of colistin resistance mechanisms in A. baumannii.",
journal = "Computational and Structural Biotechnology Journal, Computational and Structural Biotechnology JournalComputational and Structural Biotechnology Journal",
title = "Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii",
pages = "585-574",
volume = "21",
doi = "10.1016/j.csbj.2022.12.045, 10.1016/j.csbj.2022.12.045"
}

Kabić, J., Novović, K., Kekić, D., Trudić, A., Opavski, N., Dimkić, I., Jovčić, B.,& Gajić, I.. (2023). Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. in Computational and Structural Biotechnology Journal, 21, 574-585.
https://doi.org/10.1016/j.csbj.2022.12.045

Kabić J, Novović K, Kekić D, Trudić A, Opavski N, Dimkić I, Jovčić B, Gajić I. Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii. in Computational and Structural Biotechnology Journal. 2023;21:574-585.
doi:10.1016/j.csbj.2022.12.045 .

Kabić, Jovana, Novović, Katarina, Kekić, Dušan, Trudić, Anika, Opavski, Nataša, Dimkić, Ivica, Jovčić, Branko, Gajić, Ina, "Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii" in Computational and Structural Biotechnology Journal, 21 (2023):574-585,
https://doi.org/10.1016/j.csbj.2022.12.045 . .

TY  - DATA
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Poledica, Mirjana
AU  - Nikolić, Gordana
AU  - Grujić, Bojana
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Filipić, Brankica
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1783
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1783
ER  - 

@misc{
author = "Novović, Katarina and Kuzmanović Nedeljković, Snežana and Poledica, Mirjana and Nikolić, Gordana and Grujić, Bojana and Jovčić, Branko and Kojić, Milan and Filipić, Brankica",
year = "2023",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1783"
}

Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M.,& Filipić, B.. (2023). Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184. in Frontiers in Microbiology.
https://hdl.handle.net/21.15107/rcub_imagine_1783

Novović K, Kuzmanović Nedeljković S, Poledica M, Nikolić G, Grujić B, Jovčić B, Kojić M, Filipić B. Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184. in Frontiers in Microbiology. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1783 .

Novović, Katarina, Kuzmanović Nedeljković, Snežana, Poledica, Mirjana, Nikolić, Gordana, Grujić, Bojana, Jovčić, Branko, Kojić, Milan, Filipić, Brankica, "Supplementary information for the article: Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M., & Filipić, B. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. Frontiers in Microbiology, 14. https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184" in Frontiers in Microbiology (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1783 .

TY  - CONF
AU  - Ćurčić, Jovana
AU  - Jakovljević, Stefan
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
AU  - Malešević, Milka
PY  - 2023
UR  - http://intor.torlakinstitut.com/handle/123456789/803
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2124
AB  - Introduction: Quorum quenching (QQ) isthe enzymatic degradation of cell-to-cellsignaling molecules.In this study, the potential of the novel YtnP lactonase, the quorum quenching enzyme derived from S.maltophilia, to reduce P. aeruginosa quorum sensing and virulence factor gene expression was investigated.Methods: MMA83 culture (adjusted to 1.5x105 CFU/ml) was treated with recombinant YtnP lactonase(final concentration 50 μg/ml) at 37°C for 12 hours under aeration. RNA isolation of the treated and untreated MMA83 culture was performed using the RNeasy Mini Kit (Qiagen, Germany) according to theprotocol. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR), was used to analyzethe effect ofYtnP lactonase on the relative mRNA levels of the LasI/LasR, RhiI/RhiR, and PQS signaling network genes of P. aeruginosa MMA83 and virulence factor genes. The rpsL was used as an endogenouscontrol to normalize obtained data following the 2-ΔΔCt method.Results: The QS genes belonging to three QS networks – LasI/LasR, RhiI/RhiR, and PQS of P. aeruginosaMMA83 treated with YtnP lactonase were significantly downregulated. The RT -qPCR results show thattreatment with YtnP-lactonase decreased the relative mRNA levels of genes involved in the productionof elastase (lasB approximately 2-fold), alginate (algK approximately 2.2-fold), pyocyanin (phzM approximately 3.5-fold), pyoverdin (pvdS approximately 2-fold), and rhamnolipid (rhlC approximately 4-fold).These results suggest that YtnP lactonase exerts an antivirulence effect at the transcription level.Conclusion: YtnP lactonase, a quorum quenching (QQ) enzyme, has the potential to be used as an innovative enzyme-based antivirulence therapeutic to combat infections caused by P. aeruginosa.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression
EP  - 121
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2124
ER  - 

@conference{
author = "Ćurčić, Jovana and Jakovljević, Stefan and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko and Malešević, Milka",
year = "2023",
abstract = "Introduction: Quorum quenching (QQ) isthe enzymatic degradation of cell-to-cellsignaling molecules.In this study, the potential of the novel YtnP lactonase, the quorum quenching enzyme derived from S.maltophilia, to reduce P. aeruginosa quorum sensing and virulence factor gene expression was investigated.Methods: MMA83 culture (adjusted to 1.5x105 CFU/ml) was treated with recombinant YtnP lactonase(final concentration 50 μg/ml) at 37°C for 12 hours under aeration. RNA isolation of the treated and untreated MMA83 culture was performed using the RNeasy Mini Kit (Qiagen, Germany) according to theprotocol. Quantitative reverse transcription-polymerase chain reaction (RT-qPCR), was used to analyzethe effect ofYtnP lactonase on the relative mRNA levels of the LasI/LasR, RhiI/RhiR, and PQS signaling network genes of P. aeruginosa MMA83 and virulence factor genes. The rpsL was used as an endogenouscontrol to normalize obtained data following the 2-ΔΔCt method.Results: The QS genes belonging to three QS networks – LasI/LasR, RhiI/RhiR, and PQS of P. aeruginosaMMA83 treated with YtnP lactonase were significantly downregulated. The RT -qPCR results show thattreatment with YtnP-lactonase decreased the relative mRNA levels of genes involved in the productionof elastase (lasB approximately 2-fold), alginate (algK approximately 2.2-fold), pyocyanin (phzM approximately 3.5-fold), pyoverdin (pvdS approximately 2-fold), and rhamnolipid (rhlC approximately 4-fold).These results suggest that YtnP lactonase exerts an antivirulence effect at the transcription level.Conclusion: YtnP lactonase, a quorum quenching (QQ) enzyme, has the potential to be used as an innovative enzyme-based antivirulence therapeutic to combat infections caused by P. aeruginosa.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression",
pages = "121-121",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2124"
}

Ćurčić, J., Jakovljević, S., Novović, K., Vasiljević, Z., Kojić, M., Jovčić, B.,& Malešević, M.. (2023). A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2124

Ćurčić J, Jakovljević S, Novović K, Vasiljević Z, Kojić M, Jovčić B, Malešević M. A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:121-121.
https://hdl.handle.net/21.15107/rcub_imagine_2124 .

Ćurčić, Jovana, Jakovljević, Stefan, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, Malešević, Milka, "A novel YtnP lactonase reduces the expression of p. aeruginosa MMA83 quorum sensing andvirulence factors gene expression" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):121-121,
https://hdl.handle.net/21.15107/rcub_imagine_2124 .

TY  - JOUR
AU  - Novović, Katarina
AU  - Kuzmanović Nedeljković, Snežana
AU  - Poledica, Mirjana
AU  - Nikolić, Gordana
AU  - Grujić, Bojana
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Filipić, Brankica
PY  - 2023
UR  - https://www.frontiersin.org/articles/10.3389/fmicb.2023.1094184
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1782
AB  - Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.
T2  - Frontiers in Microbiology
T2  - Frontiers in Microbiology
T1  - Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia
VL  - 14
DO  - 10.3389/fmicb.2023.1094184
ER  - 

@article{
author = "Novović, Katarina and Kuzmanović Nedeljković, Snežana and Poledica, Mirjana and Nikolić, Gordana and Grujić, Bojana and Jovčić, Branko and Kojić, Milan and Filipić, Brankica",
year = "2023",
abstract = "Since the WHO declared the COVID-19 pandemic in March 2020, the disease has spread rapidly leading to overload of the health system and many of the patients infected with SARS-CoV-2 needed to be admitted to the intensive care unit (ICU). Around 10% of patients with the severe manifestation of COVID-19 need noninvasive or invasive mechanical ventilation, which represent a risk factor for Acinetobacter baumannii superinfection. The 64 A. baumannii isolates were recovered from COVID-19 patients admitted to ICU at General Hospital “Dr Laza K. Lazarević” Šabac, Serbia, during the period from December 2020 to February 2021. All patients required mechanical ventilation and mortality rate was 100%. The goal of this study was to evaluate antibiotic resistance profiles and virulence potential of A. baumannii isolates recovered from patients with severe form of COVID-19 who had a need for mechanical ventilation. All tested A. baumannii isolates (n = 64) were sensitive to colistin, while resistant to meropenem, imipenem, gentamicin, tobramycin, and levofloxacin according to the broth microdilution method and MDR phenotype was confirmed. In all tested isolates, representatives of international clone 2 (IC2) classified by multiplex PCR for clonal lineage identification, blaAmpC, blaOXA-51, and blaOXA-23 genes were present, as well as ISAba1 insertion sequence upstream of blaOXA-23. Clonal distribution of one dominant strain was found, but individual strains showed phenotypic differences in the level of antibiotic resistance, biofilm formation, and binding to mucin and motility. According to PFGE, four isolates were sequenced and antibiotic resistance genes as well as virulence factors genes were analyzed in these genomes. The results of this study represent the first report on virulence potential of MDR A. baumannii from hospital in Serbia.",
journal = "Frontiers in Microbiology, Frontiers in Microbiology",
title = "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia",
volume = "14",
doi = "10.3389/fmicb.2023.1094184"
}

Novović, K., Kuzmanović Nedeljković, S., Poledica, M., Nikolić, G., Grujić, B., Jovčić, B., Kojić, M.,& Filipić, B.. (2023). Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology, 14.
https://doi.org/10.3389/fmicb.2023.1094184

Novović K, Kuzmanović Nedeljković S, Poledica M, Nikolić G, Grujić B, Jovčić B, Kojić M, Filipić B. Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia. in Frontiers in Microbiology. 2023;14.
doi:10.3389/fmicb.2023.1094184 .

Novović, Katarina, Kuzmanović Nedeljković, Snežana, Poledica, Mirjana, Nikolić, Gordana, Grujić, Bojana, Jovčić, Branko, Kojić, Milan, Filipić, Brankica, "Virulence potential of multidrug-resistant Acinetobacter baumannii isolates from COVID-19 patients on mechanical ventilation: The first report from Serbia" in Frontiers in Microbiology, 14 (2023),
https://doi.org/10.3389/fmicb.2023.1094184 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Gardijan, Lazar
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1525
AB  - Signal transduction systems are the key players of bacterial adaptation and survival. The orthodox two-component signal transduction systems perceive diverse environmental stimuli and their regulatory response leads to cellular changes. Although rarely described, the unorthodox three-component systems are also implemented in the regulation of major bacterial behavior such as the virulence of clinically relevant pathogen P. aeruginosa. Previously, we described a novel three-component system in P. capeferrum WCS358 (RclSAR) where the sensor kinase RclS stimulates the intI1 transcription in stationary growth phase. In this study, using rclS knock-out mutant, we identified RclSAR regulon in P. capeferrum WCS358. The RNA sequencing revealed that activity of RclSAR signal transduction system is growth phase dependent with more pronounced regulatory potential in early stages of growth. Transcriptional analysis emphasized the role of RclSAR in global regulation and indicated the involvement of this system in regulation of diverse cellular activities such as RNA binding and metabolic and biocontrol processes. Importantly, phenotypic comparison of WCS358 wild type and Delta rclS mutant showed that RclS sensor kinase contributes to modulation of antibiotic resistance, production of AHLs and siderophore as well as host cell adherence and cytotoxicity. Finally, we proposed the improved model of interplay between RclSAR, RpoS and LasIR regulatory systems in P. capeferrum WCS358.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum
IS  - 15
VL  - 23
DO  - 10.3390/ijms23158232
ER  - 

@article{
author = "Novović, Katarina and Malešević, Milka and Dinić, Miroslav and Gardijan, Lazar and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "Signal transduction systems are the key players of bacterial adaptation and survival. The orthodox two-component signal transduction systems perceive diverse environmental stimuli and their regulatory response leads to cellular changes. Although rarely described, the unorthodox three-component systems are also implemented in the regulation of major bacterial behavior such as the virulence of clinically relevant pathogen P. aeruginosa. Previously, we described a novel three-component system in P. capeferrum WCS358 (RclSAR) where the sensor kinase RclS stimulates the intI1 transcription in stationary growth phase. In this study, using rclS knock-out mutant, we identified RclSAR regulon in P. capeferrum WCS358. The RNA sequencing revealed that activity of RclSAR signal transduction system is growth phase dependent with more pronounced regulatory potential in early stages of growth. Transcriptional analysis emphasized the role of RclSAR in global regulation and indicated the involvement of this system in regulation of diverse cellular activities such as RNA binding and metabolic and biocontrol processes. Importantly, phenotypic comparison of WCS358 wild type and Delta rclS mutant showed that RclS sensor kinase contributes to modulation of antibiotic resistance, production of AHLs and siderophore as well as host cell adherence and cytotoxicity. Finally, we proposed the improved model of interplay between RclSAR, RpoS and LasIR regulatory systems in P. capeferrum WCS358.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum",
number = "15",
volume = "23",
doi = "10.3390/ijms23158232"
}

Novović, K., Malešević, M., Dinić, M., Gardijan, L., Kojić, M.,& Jovčić, B.. (2022). RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum. in International Journal of Molecular Sciences
MDPI, Basel., 23(15).
https://doi.org/10.3390/ijms23158232

Novović K, Malešević M, Dinić M, Gardijan L, Kojić M, Jovčić B. RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum. in International Journal of Molecular Sciences. 2022;23(15).
doi:10.3390/ijms23158232 .

Novović, Katarina, Malešević, Milka, Dinić, Miroslav, Gardijan, Lazar, Kojić, Milan, Jovčić, Branko, "RclS Sensor Kinase Modulates Virulence of Pseudomonas capeferrum" in International Journal of Molecular Sciences, 23, no. 15 (2022),
https://doi.org/10.3390/ijms23158232 . .

TY  - JOUR
AU  - Rakonjac, B.
AU  - Lepšanović, Z.
AU  - Šuljagić, V.
AU  - Jovčić, Branko
AU  - Kojić, Milan
AU  - Larsen, A.R.
AU  - Urić, M.
AU  - Ćirković, I.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1544
AB  - Epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) is continually changing. Frequency of genotypes typical for community-associated MRSA (CA-MRSA) is increasing in hospitals, as well as resistance to antimicrobial agents. Moreover, different clones predominate in different geographic regions, and temporal shifts occur in the predominant clonal type. The aim of this study was to estimate the prevalence of MRSA, CAMRSA and PVL-positive MRSA isolates from patients hospitalised in the Military Medical Academy (MMA) and from outpatients, and to perform genotyping of PVL-positive MRSA isolates. MRSA isolates were obtained by standard microbiological techniques. PVL-positive MRSA were detected by single PCR. Determination of SCCmec types in MRSA isolates was done using multiplex PCR and genotyping of PVL-positive MRSA by PFGE, MLST and spa typing. The prevalence of MRSA among S. aureus isolates from different clinical specimens was 43.4%. In outpatients the prevalence of MRSA was 3.2%. SCCmec types specific for CA-MRSA were found in 26% of MRSA isolates from hospitalised patients. In groups, hospitalised patients and outpatients, the prevalence of PVL-positive MRSA isolates was 4%, and all of them harboured SCCmec type V genetic element. PFGE revealed minor differences between four groups of PVL-positive MRSA isolates, but all of them belonged to ST152, and all except one were of the t355 spa type. High prevalence of MRSA and CAMRSA in MMA, especially the presence of PVL-positive CA-MRSA, represent a serious health threat for patients. Genotype t355/ST152/SCCmec V is the dominant MRSA clone among PVL-positive CA-MRSA.
PB  - Public Library of Science
T2  - PLoS One
T1  - Predominance of t355/ST152/SCCmec V clonal type among PVL-positive MRSA isolates in a tertiary care hospital in Belgrade, Serbia
IS  - 9 September
VL  - 17
DO  - 10.1371/journal.pone.0273474
ER  - 

@article{
author = "Rakonjac, B. and Lepšanović, Z. and Šuljagić, V. and Jovčić, Branko and Kojić, Milan and Larsen, A.R. and Urić, M. and Ćirković, I.",
year = "2022",
abstract = "Epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) is continually changing. Frequency of genotypes typical for community-associated MRSA (CA-MRSA) is increasing in hospitals, as well as resistance to antimicrobial agents. Moreover, different clones predominate in different geographic regions, and temporal shifts occur in the predominant clonal type. The aim of this study was to estimate the prevalence of MRSA, CAMRSA and PVL-positive MRSA isolates from patients hospitalised in the Military Medical Academy (MMA) and from outpatients, and to perform genotyping of PVL-positive MRSA isolates. MRSA isolates were obtained by standard microbiological techniques. PVL-positive MRSA were detected by single PCR. Determination of SCCmec types in MRSA isolates was done using multiplex PCR and genotyping of PVL-positive MRSA by PFGE, MLST and spa typing. The prevalence of MRSA among S. aureus isolates from different clinical specimens was 43.4%. In outpatients the prevalence of MRSA was 3.2%. SCCmec types specific for CA-MRSA were found in 26% of MRSA isolates from hospitalised patients. In groups, hospitalised patients and outpatients, the prevalence of PVL-positive MRSA isolates was 4%, and all of them harboured SCCmec type V genetic element. PFGE revealed minor differences between four groups of PVL-positive MRSA isolates, but all of them belonged to ST152, and all except one were of the t355 spa type. High prevalence of MRSA and CAMRSA in MMA, especially the presence of PVL-positive CA-MRSA, represent a serious health threat for patients. Genotype t355/ST152/SCCmec V is the dominant MRSA clone among PVL-positive CA-MRSA.",
publisher = "Public Library of Science",
journal = "PLoS One",
title = "Predominance of t355/ST152/SCCmec V clonal type among PVL-positive MRSA isolates in a tertiary care hospital in Belgrade, Serbia",
number = "9 September",
volume = "17",
doi = "10.1371/journal.pone.0273474"
}

Rakonjac, B., Lepšanović, Z., Šuljagić, V., Jovčić, B., Kojić, M., Larsen, A.R., Urić, M.,& Ćirković, I.. (2022). Predominance of t355/ST152/SCCmec V clonal type among PVL-positive MRSA isolates in a tertiary care hospital in Belgrade, Serbia. in PLoS One
Public Library of Science., 17(9 September).
https://doi.org/10.1371/journal.pone.0273474

Rakonjac B, Lepšanović Z, Šuljagić V, Jovčić B, Kojić M, Larsen A, Urić M, Ćirković I. Predominance of t355/ST152/SCCmec V clonal type among PVL-positive MRSA isolates in a tertiary care hospital in Belgrade, Serbia. in PLoS One. 2022;17(9 September).
doi:10.1371/journal.pone.0273474 .

Rakonjac, B., Lepšanović, Z., Šuljagić, V., Jovčić, Branko, Kojić, Milan, Larsen, A.R., Urić, M., Ćirković, I., "Predominance of t355/ST152/SCCmec V clonal type among PVL-positive MRSA isolates in a tertiary care hospital in Belgrade, Serbia" in PLoS One, 17, no. 9 September (2022),
https://doi.org/10.1371/journal.pone.0273474 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Dinić, Miroslav
AU  - Stojković, Dejan
AU  - Jovčić, Branko
AU  - Soković, Marina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1566
AB  - The rising incidence of antibiotic resistant microorganisms urges novel antimicrobials development with polyphenols as appealing potential therapeutics. We aimed to reveal the most promising polyphenols among hesperetin, hesperidin, naringenin, naringin, taxifolin, rutin, isoquercitrin, morin, chlorogenic acid, ferulic acid, p-coumaric acid, and gallic acid based on antimicrobial capacity, antibiofilm potential, and lack of cytotoxicity towards HaCaT, and to further test its antivirulence mechanisms. Although the majority of studied polyphenols were able to inhibit bacterial growth and biofilm formation, the most promising activities were observed for rutin. Further investigation proved rutin's ability to prevent/eradicate Pseudomonas aeruginosa and MRSA urinary catheter biofilms. Besides reduction of biofilm biomass, rutin antibiofilm mechanisms included reduction of cell viability, exopolysaccharide, and extracellular DNA levels. Moderate reduction of bacterial adhesion to human keratinocytes upon treatment was observed. Rutin antivirulence mechanisms included an impact on P. aeruginosa protease, pyocyanin, rhamnolipid, and elastase production and the downregulation of the lasI, lasR, rhlI, rhlR, pqsA and mvfR genes. Rutin also interfered with membrane permeability. Polyphenols could repress antibiotic resistant bacteria. Rutin has shown wide antimicrobial and antibiofilm capacity employing a range of mechanisms that might be used for the development of novel antimicrobials.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence
IS  - 3
VL  - 15
DO  - 10.3390/ph15030385
ER  - 

@article{
author = "Ivanov, Marija and Novović, Katarina and Malešević, Milka and Dinić, Miroslav and Stojković, Dejan and Jovčić, Branko and Soković, Marina",
year = "2022",
abstract = "The rising incidence of antibiotic resistant microorganisms urges novel antimicrobials development with polyphenols as appealing potential therapeutics. We aimed to reveal the most promising polyphenols among hesperetin, hesperidin, naringenin, naringin, taxifolin, rutin, isoquercitrin, morin, chlorogenic acid, ferulic acid, p-coumaric acid, and gallic acid based on antimicrobial capacity, antibiofilm potential, and lack of cytotoxicity towards HaCaT, and to further test its antivirulence mechanisms. Although the majority of studied polyphenols were able to inhibit bacterial growth and biofilm formation, the most promising activities were observed for rutin. Further investigation proved rutin's ability to prevent/eradicate Pseudomonas aeruginosa and MRSA urinary catheter biofilms. Besides reduction of biofilm biomass, rutin antibiofilm mechanisms included reduction of cell viability, exopolysaccharide, and extracellular DNA levels. Moderate reduction of bacterial adhesion to human keratinocytes upon treatment was observed. Rutin antivirulence mechanisms included an impact on P. aeruginosa protease, pyocyanin, rhamnolipid, and elastase production and the downregulation of the lasI, lasR, rhlI, rhlR, pqsA and mvfR genes. Rutin also interfered with membrane permeability. Polyphenols could repress antibiotic resistant bacteria. Rutin has shown wide antimicrobial and antibiofilm capacity employing a range of mechanisms that might be used for the development of novel antimicrobials.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence",
number = "3",
volume = "15",
doi = "10.3390/ph15030385"
}

Ivanov, M., Novović, K., Malešević, M., Dinić, M., Stojković, D., Jovčić, B.,& Soković, M.. (2022). Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence. in Pharmaceuticals
MDPI, Basel., 15(3).
https://doi.org/10.3390/ph15030385

Ivanov M, Novović K, Malešević M, Dinić M, Stojković D, Jovčić B, Soković M. Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence. in Pharmaceuticals. 2022;15(3).
doi:10.3390/ph15030385 .

Ivanov, Marija, Novović, Katarina, Malešević, Milka, Dinić, Miroslav, Stojković, Dejan, Jovčić, Branko, Soković, Marina, "Polyphenols as Inhibitors of Antibiotic Resistant Bacteria-Mechanisms Underlying Rutin Interference with Bacterial Virulence" in Pharmaceuticals, 15, no. 3 (2022),
https://doi.org/10.3390/ph15030385 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malešević, Milka
AU  - Gardijan, Lazar
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1572
AB  - The rapid and appropriate response of Pseudomonas spp. to environmental fluctuations has been enabled by numerous signal transduction regulatory systems. Regulatory systems in Pseudomonas aeruginosa are organized in a complex network which provides quick and fine-tuned cellular response through regulation of virulence and antibiotic resistance determinants production. Mobile integrons represent genetic elements included in the rapid dissemination of multiple antibiotic resistance determinants. The key factor of integron dynamics is enzyme integrase. So far, global regulators LexA, RpoS and PsrA have been recognized as regulators of the intI1 transcription. In this study, we discovered novel activator of the intI1 transcription, sensor kinase RclS, in Pseudomonas putida WCS358. This regulation is limited to stationary growth phase and appears to be indirect, at least through regulation of the rpoS expression. Sensor kinase RclS is a part of novel three-component system Rcl (Roc-like) together with two response regulators, RclR and RclA. RclS acted as a negative regulator of the rclA transcription, while the role in the rclR transcription regulation could not be defined. The RclSAR regulatory system seems to be a part of complex intI1 regulatory network which includes major stress response (SOS and RpoS) regulons.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase
IS  - 1-2
VL  - 173
DO  - 10.1016/j.resmic.2021.103885
ER  - 

@article{
author = "Novović, Katarina and Malešević, Milka and Gardijan, Lazar and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "The rapid and appropriate response of Pseudomonas spp. to environmental fluctuations has been enabled by numerous signal transduction regulatory systems. Regulatory systems in Pseudomonas aeruginosa are organized in a complex network which provides quick and fine-tuned cellular response through regulation of virulence and antibiotic resistance determinants production. Mobile integrons represent genetic elements included in the rapid dissemination of multiple antibiotic resistance determinants. The key factor of integron dynamics is enzyme integrase. So far, global regulators LexA, RpoS and PsrA have been recognized as regulators of the intI1 transcription. In this study, we discovered novel activator of the intI1 transcription, sensor kinase RclS, in Pseudomonas putida WCS358. This regulation is limited to stationary growth phase and appears to be indirect, at least through regulation of the rpoS expression. Sensor kinase RclS is a part of novel three-component system Rcl (Roc-like) together with two response regulators, RclR and RclA. RclS acted as a negative regulator of the rclA transcription, while the role in the rclR transcription regulation could not be defined. The RclSAR regulatory system seems to be a part of complex intI1 regulatory network which includes major stress response (SOS and RpoS) regulons.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase",
number = "1-2",
volume = "173",
doi = "10.1016/j.resmic.2021.103885"
}

Novović, K., Malešević, M., Gardijan, L., Kojić, M.,& Jovčić, B.. (2022). Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase. in Research in Microbiology
Elsevier, Amsterdam., 173(1-2).
https://doi.org/10.1016/j.resmic.2021.103885

Novović K, Malešević M, Gardijan L, Kojić M, Jovčić B. Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase. in Research in Microbiology. 2022;173(1-2).
doi:10.1016/j.resmic.2021.103885 .

Novović, Katarina, Malešević, Milka, Gardijan, Lazar, Kojić, Milan, Jovčić, Branko, "Novel RclSAR three-component system regulates expression of the intI1 gene in the stationary growth phase" in Research in Microbiology, 173, no. 1-2 (2022),
https://doi.org/10.1016/j.resmic.2021.103885 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Malešević, Milka
AU  - Vasiljević, Zorica
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1778
AB  - Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.
PB  - Springer Science and Business Media B.V.
T2  - Folia Microbiologica
T1  - Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene
DO  - 10.1007/s12223-022-01026-8
ER  - 

@article{
author = "Filipić, Brankica and Malešević, Milka and Vasiljević, Zorica and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2022",
abstract = "Trimethoprim-sulfamethoxazole (SXT) is the preferable treatment option of the infections caused by Achromobacter spp. Our study aimed to analyze the SXT resistance of 98 Achromobacter spp. isolates from pediatric patients, among which 33 isolates were SXT-resistant. The presence of intI1 was screened by PCR and genome sequence analyses. The intI1 gene was detected in 10 of SXT-resistant isolates that had shorter intI1 PCR fragments named intI1S. Structural changes in intI1S were confirmed by genome sequencing and analyses which revealed 86 amino acids deletion in IntI1S protein compared to canonical IntI1 protein. All IntI1S isolates were of non-CF origin. Pan-genome analysis of intI1S bearing A. xylosoxidans isolates comprised 9052 genes, with the core genome consisting of 5455 protein-coding genes. Results in this study indicate that IntI1S isolates were derived from clinical settings and that cystic fibrosis (CF) patients were potential reservoirs for healthcare-associated infections that occurred in non-CF patients.",
publisher = "Springer Science and Business Media B.V.",
journal = "Folia Microbiologica",
title = "Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene",
doi = "10.1007/s12223-022-01026-8"
}

Filipić, B., Malešević, M., Vasiljević, Z., Novović, K., Kojić, M.,& Jovčić, B.. (2022). Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene. in Folia Microbiologica
Springer Science and Business Media B.V...
https://doi.org/10.1007/s12223-022-01026-8

Filipić B, Malešević M, Vasiljević Z, Novović K, Kojić M, Jovčić B. Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene. in Folia Microbiologica. 2022;.
doi:10.1007/s12223-022-01026-8 .

Filipić, Brankica, Malešević, Milka, Vasiljević, Zorica, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Comparative genomics of trimethoprim-sulfamethoxazole-resistant Achromobacter xylosoxidans clinical isolates from Serbia reveals shortened variant of class 1 integron integrase gene" in Folia Microbiologica (2022),
https://doi.org/10.1007/s12223-022-01026-8 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Malešević, Milka
AU  - Kojić, Milan
AU  - Galić, Nataša
AU  - Todorović, Dalibor
AU  - Vidanović, Dejan
AU  - Velhner, Maja
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1578
AB  - Owing to the emerging resistance to antimicrobials in Salmonella Kentucky isolates around the globe, the genomic comparison of all the registered multidrug-resistant Salmonella Kentucky isolates in Serbia (five from humans, one from turkey flock, and one from meat) was done. Most of the isolates were isolated from patients returning from Egypt or Tunisia or originated from imported turkey flock and turkey meat. The comparative analysis of resistance and virulence genes was done. All isolates belonged to sequence type-ST198 and were resistant to ciprofloxacin (Cip). The resistance to Cip was mediated by target mutations of the gyrA and parC genes, which encode topoisomerase I and II, respectively. Multidrug-resistant phenotype to aminoglycosides, beta-lactam antibiotics, sulfonamides, and tetracyclines was detected in five isolates. However, none of the isolates was pan-resistant to antimicrobials. The number of single nucleotide polymorphisms between isolates varied from 8 to 43 and phylogenomics revealed the genetic proximity of the human isolate 10475/11 and the turkey meat isolate 5264/14, indicating a possible meat-to-human transfer. All isolates belonged to the main Salmonella Kentucky MDR lineage, carrying the Salmonella genomic island 1 (SGI1-K) subtype. The SGI1-K of Serbian isolates showed mosaicism attributed to rapid intraclonal evolution. Many virulence factors were detected in all the isolates, including SPI-1, SPI-2, SPI-3, SPI-4, SPI-5, SPI-9, and C63PI. Although Salmonella Kentucky has rarely been isolated from humans, food, and animals in Serbia, further surveillance is needed to diminish the risk of the spreading of resistant clones and their meat-to-human transmission.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Foodborne Pathogens and Disease
T1  - Genomic Analysis of Multidrug-Resistant Salmonella enterica Serovar Kentucky Isolates from Humans, Turkey, and Food in the Republic of Serbia
EP  - 636
IS  - 9
SP  - 630
VL  - 19
DO  - 10.1089/fpd.2022.0029
ER  - 

@article{
author = "Jovčić, Branko and Malešević, Milka and Kojić, Milan and Galić, Nataša and Todorović, Dalibor and Vidanović, Dejan and Velhner, Maja",
year = "2022",
abstract = "Owing to the emerging resistance to antimicrobials in Salmonella Kentucky isolates around the globe, the genomic comparison of all the registered multidrug-resistant Salmonella Kentucky isolates in Serbia (five from humans, one from turkey flock, and one from meat) was done. Most of the isolates were isolated from patients returning from Egypt or Tunisia or originated from imported turkey flock and turkey meat. The comparative analysis of resistance and virulence genes was done. All isolates belonged to sequence type-ST198 and were resistant to ciprofloxacin (Cip). The resistance to Cip was mediated by target mutations of the gyrA and parC genes, which encode topoisomerase I and II, respectively. Multidrug-resistant phenotype to aminoglycosides, beta-lactam antibiotics, sulfonamides, and tetracyclines was detected in five isolates. However, none of the isolates was pan-resistant to antimicrobials. The number of single nucleotide polymorphisms between isolates varied from 8 to 43 and phylogenomics revealed the genetic proximity of the human isolate 10475/11 and the turkey meat isolate 5264/14, indicating a possible meat-to-human transfer. All isolates belonged to the main Salmonella Kentucky MDR lineage, carrying the Salmonella genomic island 1 (SGI1-K) subtype. The SGI1-K of Serbian isolates showed mosaicism attributed to rapid intraclonal evolution. Many virulence factors were detected in all the isolates, including SPI-1, SPI-2, SPI-3, SPI-4, SPI-5, SPI-9, and C63PI. Although Salmonella Kentucky has rarely been isolated from humans, food, and animals in Serbia, further surveillance is needed to diminish the risk of the spreading of resistant clones and their meat-to-human transmission.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Foodborne Pathogens and Disease",
title = "Genomic Analysis of Multidrug-Resistant Salmonella enterica Serovar Kentucky Isolates from Humans, Turkey, and Food in the Republic of Serbia",
pages = "636-630",
number = "9",
volume = "19",
doi = "10.1089/fpd.2022.0029"
}

Jovčić, B., Malešević, M., Kojić, M., Galić, N., Todorović, D., Vidanović, D.,& Velhner, M.. (2022). Genomic Analysis of Multidrug-Resistant Salmonella enterica Serovar Kentucky Isolates from Humans, Turkey, and Food in the Republic of Serbia. in Foodborne Pathogens and Disease
Mary Ann Liebert, Inc, New Rochelle., 19(9), 630-636.
https://doi.org/10.1089/fpd.2022.0029

Jovčić B, Malešević M, Kojić M, Galić N, Todorović D, Vidanović D, Velhner M. Genomic Analysis of Multidrug-Resistant Salmonella enterica Serovar Kentucky Isolates from Humans, Turkey, and Food in the Republic of Serbia. in Foodborne Pathogens and Disease. 2022;19(9):630-636.
doi:10.1089/fpd.2022.0029 .

Jovčić, Branko, Malešević, Milka, Kojić, Milan, Galić, Nataša, Todorović, Dalibor, Vidanović, Dejan, Velhner, Maja, "Genomic Analysis of Multidrug-Resistant Salmonella enterica Serovar Kentucky Isolates from Humans, Turkey, and Food in the Republic of Serbia" in Foodborne Pathogens and Disease, 19, no. 9 (2022):630-636,
https://doi.org/10.1089/fpd.2022.0029 . .

TY  - CHAP
AU  - Malešević, Milka
AU  - Jovčić, Branko
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1723
AB  - Pseudomonas aeruginosa jedan je od najznačajnijih uzročnika unutarbolničkih infekcija čiji je terapijski tretman
konvencionalnim antibioticima sve češće neefikasan usled rezistencije na antibiotike. Inovativni vidovi
kontrole infekcija, poput utišavanja međućelijske komunikacije bakterija, a time i onemogućavanja virulencije
i inhibicije patogenog fenotipa su stoga od izuzetnog značaja. U ovom radu biće predstavljena istraživanja
koja su bazirana na prirodnom svojstvu bakterija koje dele ekološke niše da sarađuju, ali i kompetiraju,
na osnovu čega su analizirane Delftia tsuruhatensis i Burkholderia cepacia koje tokom infekcija kolokalizuju
sa P. aeruginosa. Pokazano je da D. tsuruhatensis 11304 produkuje C18-HSL koji inhibira virulenciju P. aeruginosa
i rekonstituiše osetljivost na antibiotike, a takođe je po prvi put u literaturi opisano prisustvo dihidroksi-
C18-HSL u biološkim uzorcima. Opisane su i laktonaze vrste B. cepacia BCC4135 koje degraduju
autoinducere komunikacije P. aeruginosa i inhibiraju ekspresiju faktora virulencije. Utvrđena je njihova supstratna
specifičnost i ukazano na različitu biološku funkciju u zavisnosti od lokalizacije.
AB  - Pseudomonas aeruginosa is a leading cause of nosocomial infections, whose therapeutic treatment with
conventional antibiotics is increasingly ineffective due to antibiotic resistance. Inovative approaches of infection
control, such as silencing the bacterial quorum sensing system and thus virulence and pathogenic
phenotype inhibition are of great importance. In this study, there will be presented research based on natural
feature of bacteria that share the same ecological niche to coordinate, but also to compete, based on
which Delftia tsuruhatensis and Burkholderia cepacia that colocalize with P. aeruginosa during infections were
analysed. D. tsuruhatensis 11304 has been shown to produce C18-HSL which inhibits P. aeruginosa virulence
and reconstitutes antibiotic susceptibility, and the presence of dihydroxy-C18-HSL in biological samples has
also been described for the first time in the literature. B. cepacia BCC4135 lactonases that degrade autoinducers
of P. aeruginosa quorum sensing system and inhibit virulence factor expression have also been reported.
Their substrate specificity was determined and different biological function depending on their
localization was indicated.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Bbiogeni utišavači virulencije vrste Pseudomonas aeruginosa
T1  - Biogenic silencers of Pseudomonas aeruginosa virulence
EP  - 178
IS  - 1
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1723
ER  - 

@inbook{
author = "Malešević, Milka and Jovčić, Branko",
year = "2021",
abstract = "Pseudomonas aeruginosa jedan je od najznačajnijih uzročnika unutarbolničkih infekcija čiji je terapijski tretman
konvencionalnim antibioticima sve češće neefikasan usled rezistencije na antibiotike. Inovativni vidovi
kontrole infekcija, poput utišavanja međućelijske komunikacije bakterija, a time i onemogućavanja virulencije
i inhibicije patogenog fenotipa su stoga od izuzetnog značaja. U ovom radu biće predstavljena istraživanja
koja su bazirana na prirodnom svojstvu bakterija koje dele ekološke niše da sarađuju, ali i kompetiraju,
na osnovu čega su analizirane Delftia tsuruhatensis i Burkholderia cepacia koje tokom infekcija kolokalizuju
sa P. aeruginosa. Pokazano je da D. tsuruhatensis 11304 produkuje C18-HSL koji inhibira virulenciju P. aeruginosa
i rekonstituiše osetljivost na antibiotike, a takođe je po prvi put u literaturi opisano prisustvo dihidroksi-
C18-HSL u biološkim uzorcima. Opisane su i laktonaze vrste B. cepacia BCC4135 koje degraduju
autoinducere komunikacije P. aeruginosa i inhibiraju ekspresiju faktora virulencije. Utvrđena je njihova supstratna
specifičnost i ukazano na različitu biološku funkciju u zavisnosti od lokalizacije., Pseudomonas aeruginosa is a leading cause of nosocomial infections, whose therapeutic treatment with
conventional antibiotics is increasingly ineffective due to antibiotic resistance. Inovative approaches of infection
control, such as silencing the bacterial quorum sensing system and thus virulence and pathogenic
phenotype inhibition are of great importance. In this study, there will be presented research based on natural
feature of bacteria that share the same ecological niche to coordinate, but also to compete, based on
which Delftia tsuruhatensis and Burkholderia cepacia that colocalize with P. aeruginosa during infections were
analysed. D. tsuruhatensis 11304 has been shown to produce C18-HSL which inhibits P. aeruginosa virulence
and reconstitutes antibiotic susceptibility, and the presence of dihydroxy-C18-HSL in biological samples has
also been described for the first time in the literature. B. cepacia BCC4135 lactonases that degrade autoinducers
of P. aeruginosa quorum sensing system and inhibit virulence factor expression have also been reported.
Their substrate specificity was determined and different biological function depending on their
localization was indicated.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Bbiogeni utišavači virulencije vrste Pseudomonas aeruginosa, Biogenic silencers of Pseudomonas aeruginosa virulence",
pages = "178-166",
number = "1",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1723"
}

Malešević, M.,& Jovčić, B.. (2021). Bbiogeni utišavači virulencije vrste Pseudomonas aeruginosa. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(1), 166-178.
https://hdl.handle.net/21.15107/rcub_imagine_1723

Malešević M, Jovčić B. Bbiogeni utišavači virulencije vrste Pseudomonas aeruginosa. in Trendovi u molekularnoj Biologiji. 2021;(1):166-178.
https://hdl.handle.net/21.15107/rcub_imagine_1723 .

Malešević, Milka, Jovčić, Branko, "Bbiogeni utišavači virulencije vrste Pseudomonas aeruginosa" in Trendovi u molekularnoj Biologiji, no. 1 (2021):166-178,
https://hdl.handle.net/21.15107/rcub_imagine_1723 .

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Miljković, Marija
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Studholme, David J.
AU  - Kojić, Milan
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1507
AB  - Plasmids are autonomous episomally replicating genetic elements, which carry backbone genes important for the replication and maintenance within their host, and accessory genes that might confer an advantage to their host under specific selective pressure in its ecological niche. The genome of dairy isolate L. lactis subsp. lactis by. diacetylactis S50 was sequenced using the PacBio SMRT Cell Seq-RSII platform and revealed to possess one of the largest plasmidomes among L. lactis strains studied so far, harboring six plasmids: pS6 (5553 bp), pS7a (7308 bp), pS7b (7266 bp), pS19 (19,027 bp), pS74 (74,256 bp) and pS127 (127,002 bp) in total representing 8.9% of genome size (240,412 bp). Based on predicted plasmid replication proteins and origins it appears that all six plasmids replicate via the theta-type mechanism. The two the largest plasmids (pS74 and pS127), carry a number of genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as bacteriocin production, protein degradation, magnesium and cobalt/nickel transporters, selenium binding, exopolysaccharides (EPS) production, bacteriophage and stress resistance. Beside genes for replication, the small plasmids (pS6, pS7a, pS7a, and pS19) also carry genes important for mobilization and host survival such as type I restriction-modification (R-M) system, metal transporters, enzymes and transcriptional regulators. All plasmids in S50 strain are mobilizable, containing an oriT sequences, while pS127 is self-conjugative and allows for mobilization of the other plasmids. Small plasmids are prone to structural and segregational instability, while pS127 appeared to be segregationally stable thanks to the possession of two partition systems. The main characteristic of plasmid p574 is EPS production, while plasmid p5127 is characterized by proteinase and multiple bacteriocins, tra locus, phage abortive systems and metal transporters. In addition to LcnA and LcnB, plasmid p5127 encodes several bacteriocin-pheromone molecules and a new bacteriocin named LcnS50, with narrow spectrum of action limited to lactococci, that has been successfully cloned and heterologously expressed.
PB  - Elsevier, Amsterdam
T2  - International Journal of Food Microbiology
T1  - The large plasmidome of Lactococcus lactis subsp. lactis by. diacetylactis S50 confers its biotechnological properties
VL  - 337
DO  - 10.1016/j.ijfoodmicro.2020.108935
ER  - 

@article{
author = "Malešević, Milka and Stanisavljević, Nemanja and Miljković, Marija and Jovčić, Branko and Filipić, Brankica and Studholme, David J. and Kojić, Milan",
year = "2021",
abstract = "Plasmids are autonomous episomally replicating genetic elements, which carry backbone genes important for the replication and maintenance within their host, and accessory genes that might confer an advantage to their host under specific selective pressure in its ecological niche. The genome of dairy isolate L. lactis subsp. lactis by. diacetylactis S50 was sequenced using the PacBio SMRT Cell Seq-RSII platform and revealed to possess one of the largest plasmidomes among L. lactis strains studied so far, harboring six plasmids: pS6 (5553 bp), pS7a (7308 bp), pS7b (7266 bp), pS19 (19,027 bp), pS74 (74,256 bp) and pS127 (127,002 bp) in total representing 8.9% of genome size (240,412 bp). Based on predicted plasmid replication proteins and origins it appears that all six plasmids replicate via the theta-type mechanism. The two the largest plasmids (pS74 and pS127), carry a number of genes known to be important for growth and survival in the dairy environment. These genes encode technological functions such as bacteriocin production, protein degradation, magnesium and cobalt/nickel transporters, selenium binding, exopolysaccharides (EPS) production, bacteriophage and stress resistance. Beside genes for replication, the small plasmids (pS6, pS7a, pS7a, and pS19) also carry genes important for mobilization and host survival such as type I restriction-modification (R-M) system, metal transporters, enzymes and transcriptional regulators. All plasmids in S50 strain are mobilizable, containing an oriT sequences, while pS127 is self-conjugative and allows for mobilization of the other plasmids. Small plasmids are prone to structural and segregational instability, while pS127 appeared to be segregationally stable thanks to the possession of two partition systems. The main characteristic of plasmid p574 is EPS production, while plasmid p5127 is characterized by proteinase and multiple bacteriocins, tra locus, phage abortive systems and metal transporters. In addition to LcnA and LcnB, plasmid p5127 encodes several bacteriocin-pheromone molecules and a new bacteriocin named LcnS50, with narrow spectrum of action limited to lactococci, that has been successfully cloned and heterologously expressed.",
publisher = "Elsevier, Amsterdam",
journal = "International Journal of Food Microbiology",
title = "The large plasmidome of Lactococcus lactis subsp. lactis by. diacetylactis S50 confers its biotechnological properties",
volume = "337",
doi = "10.1016/j.ijfoodmicro.2020.108935"
}

Malešević, M., Stanisavljević, N., Miljković, M., Jovčić, B., Filipić, B., Studholme, D. J.,& Kojić, M.. (2021). The large plasmidome of Lactococcus lactis subsp. lactis by. diacetylactis S50 confers its biotechnological properties. in International Journal of Food Microbiology
Elsevier, Amsterdam., 337.
https://doi.org/10.1016/j.ijfoodmicro.2020.108935

Malešević M, Stanisavljević N, Miljković M, Jovčić B, Filipić B, Studholme DJ, Kojić M. The large plasmidome of Lactococcus lactis subsp. lactis by. diacetylactis S50 confers its biotechnological properties. in International Journal of Food Microbiology. 2021;337.
doi:10.1016/j.ijfoodmicro.2020.108935 .

Malešević, Milka, Stanisavljević, Nemanja, Miljković, Marija, Jovčić, Branko, Filipić, Brankica, Studholme, David J., Kojić, Milan, "The large plasmidome of Lactococcus lactis subsp. lactis by. diacetylactis S50 confers its biotechnological properties" in International Journal of Food Microbiology, 337 (2021),
https://doi.org/10.1016/j.ijfoodmicro.2020.108935 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Obradović, Mina
AU  - O'Connor, Paula M.
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1429
AB  - Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein alpha-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein alpha-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae
EP  - 1607
IS  - 10
SP  - 1595
VL  - 114
DO  - 10.1007/s10482-021-01626-3
ER  - 

@article{
author = "Mirković, Nemanja and Obradović, Mina and O'Connor, Paula M. and Filipić, Brankica and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2021",
abstract = "Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein alpha-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein alpha-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae",
pages = "1607-1595",
number = "10",
volume = "114",
doi = "10.1007/s10482-021-01626-3"
}

Mirković, N., Obradović, M., O'Connor, P. M., Filipić, B., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2021). C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 114(10), 1595-1607.
https://doi.org/10.1007/s10482-021-01626-3

Mirković N, Obradović M, O'Connor PM, Filipić B, Jovčić B, Cotter PD, Kojić M. C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2021;114(10):1595-1607.
doi:10.1007/s10482-021-01626-3 .

Mirković, Nemanja, Obradović, Mina, O'Connor, Paula M., Filipić, Brankica, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "C-protein alpha-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 114, no. 10 (2021):1595-1607,
https://doi.org/10.1007/s10482-021-01626-3 . .

TY  - JOUR
AU  - Velhner, Maja
AU  - Todorović, Dalibor
AU  - Novović, Katarina
AU  - Jovčić, Branko
AU  - Lazić, Gospava
AU  - Kojić, Milan
AU  - Kehrenberg, Corinna
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1445
AB  - Despite common resistance to antimicrobials in Escherichia coli isolates from farm animals in Serbia, no data are currently accessible on its occurrence in E. coli isolated from gulls. Therefore, 67 cloacal swabs and 70 fecal samples from black-headed gulls were investigated for the presence of antibiotic-resistant E. coli isolates. Ninety-nine isolates were obtained during the study. Resistotyping and resistance gene typing has shown that 44 isolates harbor resistance to one or more antibiotics. Multidrug resistance was detected in 24 E. coli isolates. Ten isolates were resistant to extended-spectrum cephalosporin antibiotics and were studied in detail including virulence gene typing, phylogenetic and multilocus sequence typing, and mating. These ten isolates belonged to phylogenetic groups B2 (five isolates), D (four isolates) and B1 (one isolate). Five different sequence types (ST38, ST2307, ST224, ST162 and ST34) were detected in E. coli isolates with AmpC phenotype and genotype. One isolate carried the Inc I2/FIB replicon type plasmid with the bla(CTX-M-1) gene. Nine isolates had bla(CMY-2) genes, which were detected on conjugative plasmids in seven isolates. The virulence genes hly, iroN, iss, ompT and cvaC were detected in one transconjugant. Ten isolates were found to be resistant to ciprofloxacin, whose MIC ranged from 4 to 32 mg/L. Genotyping revealed single or double mutations in the quinolone resistance determining region (QRDR) of the gyrA or gyrA, parC and parE genes, respectively. So, Black-headed gulls from Serbia may be colonized by multidrug-resistant E. coli, some of which are resistant to critically important antibiotics in medicine.
PB  - Springer, Dordrecht
T2  - Veterinary Research Communications
T1  - Characterization of antibiotic resistance in Escherichia coli isolates from Black-headed gulls (Larus ridibundus) present in the city of Novi Sad, Serbia
EP  - 209
IS  - 4
SP  - 199
VL  - 45
DO  - 10.1007/s11259-021-09801-7
ER  - 

@article{
author = "Velhner, Maja and Todorović, Dalibor and Novović, Katarina and Jovčić, Branko and Lazić, Gospava and Kojić, Milan and Kehrenberg, Corinna",
year = "2021",
abstract = "Despite common resistance to antimicrobials in Escherichia coli isolates from farm animals in Serbia, no data are currently accessible on its occurrence in E. coli isolated from gulls. Therefore, 67 cloacal swabs and 70 fecal samples from black-headed gulls were investigated for the presence of antibiotic-resistant E. coli isolates. Ninety-nine isolates were obtained during the study. Resistotyping and resistance gene typing has shown that 44 isolates harbor resistance to one or more antibiotics. Multidrug resistance was detected in 24 E. coli isolates. Ten isolates were resistant to extended-spectrum cephalosporin antibiotics and were studied in detail including virulence gene typing, phylogenetic and multilocus sequence typing, and mating. These ten isolates belonged to phylogenetic groups B2 (five isolates), D (four isolates) and B1 (one isolate). Five different sequence types (ST38, ST2307, ST224, ST162 and ST34) were detected in E. coli isolates with AmpC phenotype and genotype. One isolate carried the Inc I2/FIB replicon type plasmid with the bla(CTX-M-1) gene. Nine isolates had bla(CMY-2) genes, which were detected on conjugative plasmids in seven isolates. The virulence genes hly, iroN, iss, ompT and cvaC were detected in one transconjugant. Ten isolates were found to be resistant to ciprofloxacin, whose MIC ranged from 4 to 32 mg/L. Genotyping revealed single or double mutations in the quinolone resistance determining region (QRDR) of the gyrA or gyrA, parC and parE genes, respectively. So, Black-headed gulls from Serbia may be colonized by multidrug-resistant E. coli, some of which are resistant to critically important antibiotics in medicine.",
publisher = "Springer, Dordrecht",
journal = "Veterinary Research Communications",
title = "Characterization of antibiotic resistance in Escherichia coli isolates from Black-headed gulls (Larus ridibundus) present in the city of Novi Sad, Serbia",
pages = "209-199",
number = "4",
volume = "45",
doi = "10.1007/s11259-021-09801-7"
}

Velhner, M., Todorović, D., Novović, K., Jovčić, B., Lazić, G., Kojić, M.,& Kehrenberg, C.. (2021). Characterization of antibiotic resistance in Escherichia coli isolates from Black-headed gulls (Larus ridibundus) present in the city of Novi Sad, Serbia. in Veterinary Research Communications
Springer, Dordrecht., 45(4), 199-209.
https://doi.org/10.1007/s11259-021-09801-7

Velhner M, Todorović D, Novović K, Jovčić B, Lazić G, Kojić M, Kehrenberg C. Characterization of antibiotic resistance in Escherichia coli isolates from Black-headed gulls (Larus ridibundus) present in the city of Novi Sad, Serbia. in Veterinary Research Communications. 2021;45(4):199-209.
doi:10.1007/s11259-021-09801-7 .

Velhner, Maja, Todorović, Dalibor, Novović, Katarina, Jovčić, Branko, Lazić, Gospava, Kojić, Milan, Kehrenberg, Corinna, "Characterization of antibiotic resistance in Escherichia coli isolates from Black-headed gulls (Larus ridibundus) present in the city of Novi Sad, Serbia" in Veterinary Research Communications, 45, no. 4 (2021):199-209,
https://doi.org/10.1007/s11259-021-09801-7 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Novović, Katarina
AU  - Dekić, Svjetlana
AU  - Hrenović, Jasna
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1485
AB  - Although the molecular mechanisms of carbapenem resistance of environmental isolates ofAcinetobacter baumanniiare well described, data on the mechanisms of colistin resistance are scarce. In this study, we report the molecular mechanisms of colistin resistance in environmental isolates ofA. baumannii. Seven clinically relevant isolates ofA. baumanniibelonging to ST-2(Pasteur)were recovered from hospital wastewater and wastewater treatment plant. The phenotypic resistance to colistin was confirmed by broth microdilution with minimum inhibitory concentration values ranging from 20 to 160 mg/L. Colistin sulfate and colistimethate sodium showed bactericidal activity against two colistin-heteroresistant isolatesin vitro, but substantially recovery of population was observed after prolonged incubation.In silicogenome analysis revealed nucleotide variations resulting in amino acid changes in LpxC (N286D), LpxD (E117K), PmrB (A138T, R263S, L267W, Q309P, and A444V), and EptA (F166L, I228V, R348K, A370S, and K531T). According to reverse transcription quantitative PCR, all isolates had increased levels ofeptAmRNA and decreased levels oflpxAandlpxDmRNA. Isolates expressed low hydrophobicity, biofilm, and pellicle formation, but showed excellent survival in river water during 50 days of monitoring. Colistin- and pandrug-resistantA. baumanniidisseminated in the environment could represent the source for the occurrence of serious community-acquired infections.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Microbial Drug Resistance
T1  - Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii
EP  - 336
IS  - 3
SP  - 328
VL  - 27
DO  - 10.1089/mdr.2020.0188
ER  - 

@article{
author = "Jovčić, Branko and Novović, Katarina and Dekić, Svjetlana and Hrenović, Jasna",
year = "2021",
abstract = "Although the molecular mechanisms of carbapenem resistance of environmental isolates ofAcinetobacter baumanniiare well described, data on the mechanisms of colistin resistance are scarce. In this study, we report the molecular mechanisms of colistin resistance in environmental isolates ofA. baumannii. Seven clinically relevant isolates ofA. baumanniibelonging to ST-2(Pasteur)were recovered from hospital wastewater and wastewater treatment plant. The phenotypic resistance to colistin was confirmed by broth microdilution with minimum inhibitory concentration values ranging from 20 to 160 mg/L. Colistin sulfate and colistimethate sodium showed bactericidal activity against two colistin-heteroresistant isolatesin vitro, but substantially recovery of population was observed after prolonged incubation.In silicogenome analysis revealed nucleotide variations resulting in amino acid changes in LpxC (N286D), LpxD (E117K), PmrB (A138T, R263S, L267W, Q309P, and A444V), and EptA (F166L, I228V, R348K, A370S, and K531T). According to reverse transcription quantitative PCR, all isolates had increased levels ofeptAmRNA and decreased levels oflpxAandlpxDmRNA. Isolates expressed low hydrophobicity, biofilm, and pellicle formation, but showed excellent survival in river water during 50 days of monitoring. Colistin- and pandrug-resistantA. baumanniidisseminated in the environment could represent the source for the occurrence of serious community-acquired infections.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Microbial Drug Resistance",
title = "Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii",
pages = "336-328",
number = "3",
volume = "27",
doi = "10.1089/mdr.2020.0188"
}

Jovčić, B., Novović, K., Dekić, S.,& Hrenović, J.. (2021). Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii. in Microbial Drug Resistance
Mary Ann Liebert, Inc, New Rochelle., 27(3), 328-336.
https://doi.org/10.1089/mdr.2020.0188

Jovčić B, Novović K, Dekić S, Hrenović J. Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii. in Microbial Drug Resistance. 2021;27(3):328-336.
doi:10.1089/mdr.2020.0188 .

Jovčić, Branko, Novović, Katarina, Dekić, Svjetlana, Hrenović, Jasna, "Colistin Resistance in Environmental Isolates ofAcinetobacter baumannii" in Microbial Drug Resistance, 27, no. 3 (2021):328-336,
https://doi.org/10.1089/mdr.2020.0188 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Studholme, David J.
AU  - Malešević, Milka
AU  - Mirković, Nemanja
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1306
AB  - Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38clusters ofattCsiteslackingintegron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.
PB  - IWA Publishing, London
T2  - Journal of Water and Health
T1  - Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments
EP  - 397
IS  - 3
SP  - 383
VL  - 18
DO  - 10.2166/wh.2020.227
ER  - 

@article{
author = "Filipić, Brankica and Novović, Katarina and Studholme, David J. and Malešević, Milka and Mirković, Nemanja and Kojić, Milan and Jovčić, Branko",
year = "2020",
abstract = "Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38clusters ofattCsiteslackingintegron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.",
publisher = "IWA Publishing, London",
journal = "Journal of Water and Health",
title = "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments",
pages = "397-383",
number = "3",
volume = "18",
doi = "10.2166/wh.2020.227"
}

Filipić, B., Novović, K., Studholme, D. J., Malešević, M., Mirković, N., Kojić, M.,& Jovčić, B.. (2020). Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of Water and Health
IWA Publishing, London., 18(3), 383-397.
https://doi.org/10.2166/wh.2020.227

Filipić B, Novović K, Studholme DJ, Malešević M, Mirković N, Kojić M, Jovčić B. Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of Water and Health. 2020;18(3):383-397.
doi:10.2166/wh.2020.227 .

Filipić, Brankica, Novović, Katarina, Studholme, David J., Malešević, Milka, Mirković, Nemanja, Kojić, Milan, Jovčić, Branko, "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments" in Journal of Water and Health, 18, no. 3 (2020):383-397,
https://doi.org/10.2166/wh.2020.227 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Velhner, Maja
AU  - Todorović, Dalibor
AU  - Matović, Kazimir
AU  - Rasić, Zoran
AU  - Nikolić, Sonja
AU  - Kiskarolj, Ferenc
AU  - Kojić, Milan
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1349
AB  - The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
PB  - MDPI, Basel
T2  - Antibiotics-Basel
T1  - Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia
IS  - 12
VL  - 9
DO  - 10.3390/antibiotics9120886
ER  - 

@article{
author = "Jovčić, Branko and Novović, Katarina and Filipić, Brankica and Velhner, Maja and Todorović, Dalibor and Matović, Kazimir and Rasić, Zoran and Nikolić, Sonja and Kiskarolj, Ferenc and Kojić, Milan",
year = "2020",
abstract = "The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.",
publisher = "MDPI, Basel",
journal = "Antibiotics-Basel",
title = "Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia",
number = "12",
volume = "9",
doi = "10.3390/antibiotics9120886"
}

Jovčić, B., Novović, K., Filipić, B., Velhner, M., Todorović, D., Matović, K., Rasić, Z., Nikolić, S., Kiskarolj, F.,& Kojić, M.. (2020). Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia. in Antibiotics-Basel
MDPI, Basel., 9(12).
https://doi.org/10.3390/antibiotics9120886

Jovčić B, Novović K, Filipić B, Velhner M, Todorović D, Matović K, Rasić Z, Nikolić S, Kiskarolj F, Kojić M. Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia. in Antibiotics-Basel. 2020;9(12).
doi:10.3390/antibiotics9120886 .

Jovčić, Branko, Novović, Katarina, Filipić, Brankica, Velhner, Maja, Todorović, Dalibor, Matović, Kazimir, Rasić, Zoran, Nikolić, Sonja, Kiskarolj, Ferenc, Kojić, Milan, "Genomic Characteristics of Colistin-Resistant Salmonella enterica subsp. enterica Serovar Infantis from Poultry Farms in the Republic of Serbia" in Antibiotics-Basel, 9, no. 12 (2020),
https://doi.org/10.3390/antibiotics9120886 . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Stanisavljević, Nemanja
AU  - Novović, Katarina
AU  - Polović, Natalija
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1338
AB  - Burkholderia cepacia is well known as the causative agent of infections in humans where often shares niche with other pathogens, like Pseudomonas aeruginosa. Clinical isolate Burkholderia sp. BCC4135 was selected due to its strong quorum quenching (QQ) activity. Whole genome sequencing unveiled this isolate as B. cepacia with unique sequence type ST1485 and a myriad of genes belonging to resistome and virulome. Two QQ lactonases YtnP and Y2-aiiA originated from B. cepacia BCC4135 were cloned, expressed, and functionally characterized. They were active against a broad substrate spectrum of the N-acyl-homoserine lactones (AHLs). The YtnP lactonase was inactive, while Y2-aiiA was active against N-tetradecanoyl-DL-homoserine lactone (C14-HSL) which could imply the difference in their biological roles from the aspect of its quorum sensing (QS) autoregulation and interference with the QS systems of bacteria residing within the same niche. Both YtnP and Y2-aiiA were able to attenuate virulence potential of P. aeruginosa MMA83 clinical isolate declining its biofilm formation and virulence factors production. B. cepacia BCC4135 lactonases interfered with the las, rhl, and even pqs QS circuit of P. aeruginosa MMA83 transcription and the effect of combined enzymes was even more prominent. B. cepacia BCC4135 also employs the CepI/R QS system for governing its own virulence traits and possibly self-regulates the QQ/QS network through the different expression and activity of YtnP and/or Y2-aiiA. Our findings pointed out that BCC4135 lactonases could be exploited as an effective antivirulence drugs against P. aeruginosa and gave us a new insight into B. cepacia QQ/QS machinery.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity
VL  - 149
DO  - 10.1016/j.micpath.2020.104561
ER  - 

@article{
author = "Malešević, Milka and Stanisavljević, Nemanja and Novović, Katarina and Polović, Natalija and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko",
year = "2020",
abstract = "Burkholderia cepacia is well known as the causative agent of infections in humans where often shares niche with other pathogens, like Pseudomonas aeruginosa. Clinical isolate Burkholderia sp. BCC4135 was selected due to its strong quorum quenching (QQ) activity. Whole genome sequencing unveiled this isolate as B. cepacia with unique sequence type ST1485 and a myriad of genes belonging to resistome and virulome. Two QQ lactonases YtnP and Y2-aiiA originated from B. cepacia BCC4135 were cloned, expressed, and functionally characterized. They were active against a broad substrate spectrum of the N-acyl-homoserine lactones (AHLs). The YtnP lactonase was inactive, while Y2-aiiA was active against N-tetradecanoyl-DL-homoserine lactone (C14-HSL) which could imply the difference in their biological roles from the aspect of its quorum sensing (QS) autoregulation and interference with the QS systems of bacteria residing within the same niche. Both YtnP and Y2-aiiA were able to attenuate virulence potential of P. aeruginosa MMA83 clinical isolate declining its biofilm formation and virulence factors production. B. cepacia BCC4135 lactonases interfered with the las, rhl, and even pqs QS circuit of P. aeruginosa MMA83 transcription and the effect of combined enzymes was even more prominent. B. cepacia BCC4135 also employs the CepI/R QS system for governing its own virulence traits and possibly self-regulates the QQ/QS network through the different expression and activity of YtnP and/or Y2-aiiA. Our findings pointed out that BCC4135 lactonases could be exploited as an effective antivirulence drugs against P. aeruginosa and gave us a new insight into B. cepacia QQ/QS machinery.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity",
volume = "149",
doi = "10.1016/j.micpath.2020.104561"
}

Malešević, M., Stanisavljević, N., Novović, K., Polović, N., Vasiljević, Z., Kojić, M.,& Jovčić, B.. (2020). Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 149.
https://doi.org/10.1016/j.micpath.2020.104561

Malešević M, Stanisavljević N, Novović K, Polović N, Vasiljević Z, Kojić M, Jovčić B. Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity. in Microbial Pathogenesis. 2020;149.
doi:10.1016/j.micpath.2020.104561 .

Malešević, Milka, Stanisavljević, Nemanja, Novović, Katarina, Polović, Natalija, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, "Burkholderia cepacia YtnP and Y2-aiiA lactonases inhibit virulence of Pseudomonas aeruginosa via quorum quenching activity" in Microbial Pathogenesis, 149 (2020),
https://doi.org/10.1016/j.micpath.2020.104561 . .

TY  - JOUR
AU  - Vukotić, Goran
AU  - Obradović, Mina
AU  - Novović, Katarina
AU  - Di Luca, Mariagrazia
AU  - Jovčić, Branko
AU  - Fira, Đorđe
AU  - Neve, Horst
AU  - Kojić, Milan
AU  - McAuliffe, Olivia
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1355
AB  - Acinetobacter baumanniiis a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistantA. baumanniistrain 6077/12, vB_AbaM_ISTD, and vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified, and concentrated using CsCl gradient ultracentrifugation. The phages were screened against 103 clinical isolates ofA. baumanniifrom a laboratory collection and characterized based on plaque and virion morphology, host range, adsorption rate, and one-step growth curve. Given that phage ISTD showed a broader host range, better adsorption rate, shorter latent period, and larger burst size, its ability to lyse planktonic and biofilm-embedded cells was tested in detail. Phage ISTD yielded a 3.5- and 2-log reduction in planktonic and biofilm-associated viable bacterial cell count, respectively, but the effect was time-dependent. Both phages produced growing turbid halos around plaques indicating the synthesis of depolymerases, enzymes capable of degrading bacterial exopolysaccharides. Halos tested positive for presence of phages in the proximity of the plaque, but not further from the plaque, which indicates that the observed halo enlargement is a consequence of enzyme diffusion through the agar, independently of the phages. This notion was also supported by the growing halos induced by phage preparations applied on pregrown bacterial lawns, indicating that depolymerizing effect was achieved also on non-dividing sensitive cells. Overall, good rates of growth, fast adsorption rate, broad host range, and high depolymerizing activity, as well as antibacterial effectiveness against planktonic and biofilm-associated bacteria, make these phages good candidates for potential application in combatingA. baumanniiinfections.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Medicine
T1  - Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii
VL  - 7
DO  - 10.3389/fmed.2020.00426
ER  - 

@article{
author = "Vukotić, Goran and Obradović, Mina and Novović, Katarina and Di Luca, Mariagrazia and Jovčić, Branko and Fira, Đorđe and Neve, Horst and Kojić, Milan and McAuliffe, Olivia",
year = "2020",
abstract = "Acinetobacter baumanniiis a leading cause of healthcare-associated infections worldwide. Its various intrinsic and acquired mechanisms of antibiotic resistance make the therapeutic challenge even more serious. One of the promising alternative treatments that is increasingly highlighted is phage therapy, the therapeutic use of bacteriophages to treat bacterial infections. Two phages active against nosocomial carbapenem-resistantA. baumanniistrain 6077/12, vB_AbaM_ISTD, and vB_AbaM_NOVI, were isolated from Belgrade wastewaters, purified, and concentrated using CsCl gradient ultracentrifugation. The phages were screened against 103 clinical isolates ofA. baumanniifrom a laboratory collection and characterized based on plaque and virion morphology, host range, adsorption rate, and one-step growth curve. Given that phage ISTD showed a broader host range, better adsorption rate, shorter latent period, and larger burst size, its ability to lyse planktonic and biofilm-embedded cells was tested in detail. Phage ISTD yielded a 3.5- and 2-log reduction in planktonic and biofilm-associated viable bacterial cell count, respectively, but the effect was time-dependent. Both phages produced growing turbid halos around plaques indicating the synthesis of depolymerases, enzymes capable of degrading bacterial exopolysaccharides. Halos tested positive for presence of phages in the proximity of the plaque, but not further from the plaque, which indicates that the observed halo enlargement is a consequence of enzyme diffusion through the agar, independently of the phages. This notion was also supported by the growing halos induced by phage preparations applied on pregrown bacterial lawns, indicating that depolymerizing effect was achieved also on non-dividing sensitive cells. Overall, good rates of growth, fast adsorption rate, broad host range, and high depolymerizing activity, as well as antibacterial effectiveness against planktonic and biofilm-associated bacteria, make these phages good candidates for potential application in combatingA. baumanniiinfections.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Medicine",
title = "Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii",
volume = "7",
doi = "10.3389/fmed.2020.00426"
}

Vukotić, G., Obradović, M., Novović, K., Di Luca, M., Jovčić, B., Fira, Đ., Neve, H., Kojić, M.,& McAuliffe, O.. (2020). Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii. in Frontiers in Medicine
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fmed.2020.00426

Vukotić G, Obradović M, Novović K, Di Luca M, Jovčić B, Fira Đ, Neve H, Kojić M, McAuliffe O. Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii. in Frontiers in Medicine. 2020;7.
doi:10.3389/fmed.2020.00426 .

Vukotić, Goran, Obradović, Mina, Novović, Katarina, Di Luca, Mariagrazia, Jovčić, Branko, Fira, Đorđe, Neve, Horst, Kojić, Milan, McAuliffe, Olivia, "Characterization, Antibiofilm, and Depolymerizing Activity of Two Phages Active on Carbapenem-ResistantAcinetobacter baumannii" in Frontiers in Medicine, 7 (2020),
https://doi.org/10.3389/fmed.2020.00426 . .