TY  - CONF
AU  - Janković, Vukašin
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Radetić, Maja
AU  - Marković, Darka
AU  - Kalogirou, Charalampia
AU  - Ilić-Tomić, Tatjana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2371
AB  - The increasing production and utilization of
synthetic polymers in the textile industry over
the past five decades has raised concerns about
the environmental impact of the industry. The
recalcitrant nature of synthetic fibers hampers
the biodegradation of these textiles in the environment
and leads to the accumulation of textile
waste. Effective solutions for recycling and proper
disposal of textile waste are lacking, however,
the use of microorganisms and enzymes has
emerged as a promising approach. The genus
Streptomyces has been well studied as a producer
of different hydrolytic enzymes, several of which
have found use in industrial settings as well. As
an integral part of the soil microbiome, Streptomyces
species have been shown to interact with
different textile materials in soil and may play a
role in the degradation of these materials. This
study aimed to examine the interaction of Streptomyces
sp. R1, isolated from the rhizosphere of
Cotinus coggygria, with polyamide/polyurethane
textile, and identify potential enzymes involved in the biodegradation of synthetic textiles. The
degradation of the textile was tested in liquid
cultures (minimal salt medium) and model compost,
bio-augmented with Streptomyces sp. R1
for 4 months. After the incubation, morphological,
and changes in the functional groups of the
textiles were analysed using scanning electron
microscopy (SEM) and Fourier transform infrared
spectroscopy (FTIR). The surface of the textile
showed noticeable cracks and fissures after
4 months of burial in the bioaugmented model
compost, alongside changes in the functional
groups of the polyamide/polyurethane textile,
which indicates biodegradation of the synthetic
fibers. Searching the genome of Streptomyces sp.
R1, several enzymes involved in the degradation
of synthetic polymers were identified, including
an esterase homologous to highly efficient plastic
degrading depolymerases. Overall, the results
presented here indicate Streptomyces sp. R1 has
the potential for synthetic textile degradation
and bioremediation.
PB  - Serbian Society for Microbiology
C3  - XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
T1  - DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2371
ER  - 

@conference{
author = "Janković, Vukašin and Pantelić, Brana and Jeremić, Sanja and Radetić, Maja and Marković, Darka and Kalogirou, Charalampia and Ilić-Tomić, Tatjana",
year = "2024",
abstract = "The increasing production and utilization of
synthetic polymers in the textile industry over
the past five decades has raised concerns about
the environmental impact of the industry. The
recalcitrant nature of synthetic fibers hampers
the biodegradation of these textiles in the environment
and leads to the accumulation of textile
waste. Effective solutions for recycling and proper
disposal of textile waste are lacking, however,
the use of microorganisms and enzymes has
emerged as a promising approach. The genus
Streptomyces has been well studied as a producer
of different hydrolytic enzymes, several of which
have found use in industrial settings as well. As
an integral part of the soil microbiome, Streptomyces
species have been shown to interact with
different textile materials in soil and may play a
role in the degradation of these materials. This
study aimed to examine the interaction of Streptomyces
sp. R1, isolated from the rhizosphere of
Cotinus coggygria, with polyamide/polyurethane
textile, and identify potential enzymes involved in the biodegradation of synthetic textiles. The
degradation of the textile was tested in liquid
cultures (minimal salt medium) and model compost,
bio-augmented with Streptomyces sp. R1
for 4 months. After the incubation, morphological,
and changes in the functional groups of the
textiles were analysed using scanning electron
microscopy (SEM) and Fourier transform infrared
spectroscopy (FTIR). The surface of the textile
showed noticeable cracks and fissures after
4 months of burial in the bioaugmented model
compost, alongside changes in the functional
groups of the polyamide/polyurethane textile,
which indicates biodegradation of the synthetic
fibers. Searching the genome of Streptomyces sp.
R1, several enzymes involved in the degradation
of synthetic polymers were identified, including
an esterase homologous to highly efficient plastic
degrading depolymerases. Overall, the results
presented here indicate Streptomyces sp. R1 has
the potential for synthetic textile degradation
and bioremediation.",
publisher = "Serbian Society for Microbiology",
journal = "XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health",
title = "DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2371"
}

Janković, V., Pantelić, B., Jeremić, S., Radetić, M., Marković, D., Kalogirou, C.,& Ilić-Tomić, T.. (2024). DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health
Serbian Society for Microbiology., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2371

Janković V, Pantelić B, Jeremić S, Radetić M, Marković D, Kalogirou C, Ilić-Tomić T. DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1. in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health. 2024;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2371 .

Janković, Vukašin, Pantelić, Brana, Jeremić, Sanja, Radetić, Maja, Marković, Darka, Kalogirou, Charalampia, Ilić-Tomić, Tatjana, "DEGRADATION OF POLYAMIDE/POLYURETHANE TEXTILE BLEND BY STREPTOMYCES SP. R1" in XIII Congress of microbiologists of Serbia: From biotechnology to human and planetary health (2024):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2371 .

TY  - JOUR
AU  - Araujo, Jeovan A.
AU  - Taxeidis, George
AU  - Pereira, Everton H.
AU  - Azeem, Muhammad
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Ponjavić, Marijana
AU  - Chen, Yuanyuan
AU  - Mojicević, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
AU  - Brennan Fournet, Margaret
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0959652624004724
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2315
AB  - Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.
PB  - Elsevier
T2  - Journal of Cleaner Production
T1  - Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose
SP  - 141025
DO  - 10.1016/j.jclepro.2024.141025
ER  - 

@article{
author = "Araujo, Jeovan A. and Taxeidis, George and Pereira, Everton H. and Azeem, Muhammad and Pantelić, Brana and Jeremić, Sanja and Ponjavić, Marijana and Chen, Yuanyuan and Mojicević, Marija and Nikodinović-Runić, Jasmina and Topakas, Evangelos and Brennan Fournet, Margaret",
year = "2024",
abstract = "Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.",
publisher = "Elsevier",
journal = "Journal of Cleaner Production",
title = "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose",
pages = "141025",
doi = "10.1016/j.jclepro.2024.141025"
}

Araujo, J. A., Taxeidis, G., Pereira, E. H., Azeem, M., Pantelić, B., Jeremić, S., Ponjavić, M., Chen, Y., Mojicević, M., Nikodinović-Runić, J., Topakas, E.,& Brennan Fournet, M.. (2024). Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production
Elsevier., 141025.
https://doi.org/10.1016/j.jclepro.2024.141025

Araujo JA, Taxeidis G, Pereira EH, Azeem M, Pantelić B, Jeremić S, Ponjavić M, Chen Y, Mojicević M, Nikodinović-Runić J, Topakas E, Brennan Fournet M. Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production. 2024;:141025.
doi:10.1016/j.jclepro.2024.141025 .

Araujo, Jeovan A., Taxeidis, George, Pereira, Everton H., Azeem, Muhammad, Pantelić, Brana, Jeremić, Sanja, Ponjavić, Marijana, Chen, Yuanyuan, Mojicević, Marija, Nikodinović-Runić, Jasmina, Topakas, Evangelos, Brennan Fournet, Margaret, "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose" in Journal of Cleaner Production (2024):141025,
https://doi.org/10.1016/j.jclepro.2024.141025 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Cosović, Vladan
AU  - Atanase, Leonard Ionut
AU  - Freitas, Filomena
AU  - Matos, Mariana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1714
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
IS  - 3
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Cosović, Vladan and Atanase, Leonard Ionut and Freitas, Filomena and Matos, Mariana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
number = "3",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Cosović, V., Atanase, L. I., Freitas, F., Matos, M.,& Nikodinović-Runić, J.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences, 24(3), 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Cosović V, Atanase LI, Freitas F, Matos M, Nikodinović-Runić J. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24(3):1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Cosović, Vladan, Atanase, Leonard Ionut, Freitas, Filomena, Matos, Mariana, Nikodinović-Runić, Jasmina, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24, no. 3 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Araujo, Jeovan
AU  - Jeremić, Sanja
AU  - Azeem, Muhammad
AU  - Attallah, Olivia
AU  - Slaperas, Romanos
AU  - Mojicević, Marija
AU  - Chen, Yuanyuan
AU  - Fournet, Margaret Brennan
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2352186423003127
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1983
AB  - Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.
PB  - Elsevier
T2  - Environmental Technology & Innovation
T1  - A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate
SP  - 103316
DO  - 10.1016/j.eti.2023.103316
ER  - 

@article{
author = "Pantelić, Brana and Araujo, Jeovan and Jeremić, Sanja and Azeem, Muhammad and Attallah, Olivia and Slaperas, Romanos and Mojicević, Marija and Chen, Yuanyuan and Fournet, Margaret Brennan and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.",
publisher = "Elsevier",
journal = "Environmental Technology & Innovation",
title = "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate",
pages = "103316",
doi = "10.1016/j.eti.2023.103316"
}

Pantelić, B., Araujo, J., Jeremić, S., Azeem, M., Attallah, O., Slaperas, R., Mojicević, M., Chen, Y., Fournet, M. B., Topakas, E.,& Nikodinović-Runić, J.. (2023). A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation
Elsevier., 103316.
https://doi.org/10.1016/j.eti.2023.103316

Pantelić B, Araujo J, Jeremić S, Azeem M, Attallah O, Slaperas R, Mojicević M, Chen Y, Fournet MB, Topakas E, Nikodinović-Runić J. A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation. 2023;:103316.
doi:10.1016/j.eti.2023.103316 .

Pantelić, Brana, Araujo, Jeovan, Jeremić, Sanja, Azeem, Muhammad, Attallah, Olivia, Slaperas, Romanos, Mojicević, Marija, Chen, Yuanyuan, Fournet, Margaret Brennan, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate" in Environmental Technology & Innovation (2023):103316,
https://doi.org/10.1016/j.eti.2023.103316 . .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Jeremić, Sanja
AU  - Malagurski, Ivana
AU  - Babu P., Ramesh
AU  - Rajasekaran, Divya
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://afea.eventsair.com/10th-conference-of-mikrobiokosmos/abstract-book
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2236
AB  - The rapid increase in global plastics production is
also causing an accelerated environmental
pollution. Recently, biotechnological solutions and
enzymatic recycling of poly(ethylene terephthalate)
(PET) waste stream have been put forward and
commercialized1. Increasing recycling and
upcycling rates is the most effective model
approach to plastic circularity. However, mixed
plastic waste is still quite a challenge for both
recycling and upcycling technologies. This study is
focused on the eco-conversion of plastic waste
containing poly(ethylene terephthalate), PET, into
biopolymer, bacterial nanocellulose. Polymer mix
contained selection of commercial biodegradable
plastics (poly(lactic acid), PLA, poly(ε-caprolactone),
PCL, poly(hyoxyl butyrate), PHB) and PET. This
mixture was hydrolysed under aqueous conditions
and hydrolysate was used as carbon source for
Komagataeibacter medellinensis ID13488 and
bacterial nanocellulose (BNC) production. HPLC
analysis confirmed the presence of monomers and
dimers of polymer mix components indicating
existence of potential substrates for BNC
production. BNC production by K. medellinensis
was investigated and optimized in ter of the
amount of carbon source and growth conditions.
Under the most efficient rate in ter of yield, BNC
production was scaled up and the obtained
biopolymer was characterized. The structure of
produced BNC was confirmed by FTIR analysis,
thermal properties by DSC/TG analysis, and the
morphology of material by optical microscopy and
SEM analysis. This research demonstrates how to
put the mixed plastic waste stream into a circular
loop through the biotechnological conversion into
valuable biopolymer.
C3  - 10th Conference of Mikrobiokosmos
T1  - Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2236
ER  - 

@conference{
author = "Ponjavić, Marijana and Jeremić, Sanja and Malagurski, Ivana and Babu P., Ramesh and Rajasekaran, Divya and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The rapid increase in global plastics production is
also causing an accelerated environmental
pollution. Recently, biotechnological solutions and
enzymatic recycling of poly(ethylene terephthalate)
(PET) waste stream have been put forward and
commercialized1. Increasing recycling and
upcycling rates is the most effective model
approach to plastic circularity. However, mixed
plastic waste is still quite a challenge for both
recycling and upcycling technologies. This study is
focused on the eco-conversion of plastic waste
containing poly(ethylene terephthalate), PET, into
biopolymer, bacterial nanocellulose. Polymer mix
contained selection of commercial biodegradable
plastics (poly(lactic acid), PLA, poly(ε-caprolactone),
PCL, poly(hyoxyl butyrate), PHB) and PET. This
mixture was hydrolysed under aqueous conditions
and hydrolysate was used as carbon source for
Komagataeibacter medellinensis ID13488 and
bacterial nanocellulose (BNC) production. HPLC
analysis confirmed the presence of monomers and
dimers of polymer mix components indicating
existence of potential substrates for BNC
production. BNC production by K. medellinensis
was investigated and optimized in ter of the
amount of carbon source and growth conditions.
Under the most efficient rate in ter of yield, BNC
production was scaled up and the obtained
biopolymer was characterized. The structure of
produced BNC was confirmed by FTIR analysis,
thermal properties by DSC/TG analysis, and the
morphology of material by optical microscopy and
SEM analysis. This research demonstrates how to
put the mixed plastic waste stream into a circular
loop through the biotechnological conversion into
valuable biopolymer.",
journal = "10th Conference of Mikrobiokosmos",
title = "Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2236"
}

Ponjavić, M., Jeremić, S., Malagurski, I., Babu P., R., Rajasekaran, D., Topakas, E.,& Nikodinović-Runić, J.. (2023). Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose. in 10th Conference of Mikrobiokosmos.
https://hdl.handle.net/21.15107/rcub_imagine_2236

Ponjavić M, Jeremić S, Malagurski I, Babu P. R, Rajasekaran D, Topakas E, Nikodinović-Runić J. Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose. in 10th Conference of Mikrobiokosmos. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2236 .

Ponjavić, Marijana, Jeremić, Sanja, Malagurski, Ivana, Babu P., Ramesh, Rajasekaran, Divya, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Conversion of mixed plastic waste containing PET into biopolymer bacterial nanocellulose" in 10th Conference of Mikrobiokosmos (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2236 .

TY  - CONF
AU  - Jeremić, Sanja
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2144
AB  - Introduction: Fossil-based polymers continue to be widely used despite their negative environmental
impact. Bioplastics, such as polylactic acid (PLA), offer a promising alternative as they are derived from
renewable resources and provide more environmentally friendly end-of-life options. However, marketing PLA assimply biodegradable can be misleading, asthe current PLA degradation strategy contributes
to microplastics pollution, thus posing even greater threat. Thisresearch focuses on the upcycling of PLA
degradation products into valuable biomaterial - bacterial nanocellulose.
Methods: PLA samples were pretreated using ultraviolet and ultrasonic waves, individually and in combination, to enhancessusceptibility to bacterial degradation. Pretreated PLA wassubjected to enzymatic
degradation under mild conditions, using various enzyme combinations. The resulting biodegradation
products served as a growth medium for nanocellulose producing bacteria Komagataeibacter medellinensis ID13488. Obtained nanocellulose was characterized using SEM, FTIR, AFM, and XRD.
Results: The combined PLA pretreatment using ultraviolet and ultrasonic waves, followed by enzymatic
degradation with savinase, demonstrated the highest degree of PLA degradation in this study. Furthermore, K. medellinensis ID13488 efficiently utilized the biodegradation products, producing nanocellulose with yields and performance comparable to those obtained through standard cultivation using
glucose as a carbon source.
Conclusion: This study highlights the potential of combined pretreatment and enzymatic degradation
for efficient PLA degradation and sustainable bacterial nanocellulose production. The findings suggest
promising avenues for utilizing PLA biodegradation products in the production of other valuable biomaterials. Further research is needed to optimize the pretreatment and degradation processes, facilitating the wider application of biodegradable materials and promoting sustainability.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Bacterial nanocellulose – new beginning for end-of-life plastics
EP  - 107
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2144
ER  - 

@conference{
author = "Jeremić, Sanja",
year = "2023",
abstract = "Introduction: Fossil-based polymers continue to be widely used despite their negative environmental
impact. Bioplastics, such as polylactic acid (PLA), offer a promising alternative as they are derived from
renewable resources and provide more environmentally friendly end-of-life options. However, marketing PLA assimply biodegradable can be misleading, asthe current PLA degradation strategy contributes
to microplastics pollution, thus posing even greater threat. Thisresearch focuses on the upcycling of PLA
degradation products into valuable biomaterial - bacterial nanocellulose.
Methods: PLA samples were pretreated using ultraviolet and ultrasonic waves, individually and in combination, to enhancessusceptibility to bacterial degradation. Pretreated PLA wassubjected to enzymatic
degradation under mild conditions, using various enzyme combinations. The resulting biodegradation
products served as a growth medium for nanocellulose producing bacteria Komagataeibacter medellinensis ID13488. Obtained nanocellulose was characterized using SEM, FTIR, AFM, and XRD.
Results: The combined PLA pretreatment using ultraviolet and ultrasonic waves, followed by enzymatic
degradation with savinase, demonstrated the highest degree of PLA degradation in this study. Furthermore, K. medellinensis ID13488 efficiently utilized the biodegradation products, producing nanocellulose with yields and performance comparable to those obtained through standard cultivation using
glucose as a carbon source.
Conclusion: This study highlights the potential of combined pretreatment and enzymatic degradation
for efficient PLA degradation and sustainable bacterial nanocellulose production. The findings suggest
promising avenues for utilizing PLA biodegradation products in the production of other valuable biomaterials. Further research is needed to optimize the pretreatment and degradation processes, facilitating the wider application of biodegradable materials and promoting sustainability.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Bacterial nanocellulose – new beginning for end-of-life plastics",
pages = "107-107",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2144"
}

Jeremić, S.. (2023). Bacterial nanocellulose – new beginning for end-of-life plastics. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 107-107.
https://hdl.handle.net/21.15107/rcub_imagine_2144

Jeremić S. Bacterial nanocellulose – new beginning for end-of-life plastics. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:107-107.
https://hdl.handle.net/21.15107/rcub_imagine_2144 .

Jeremić, Sanja, "Bacterial nanocellulose – new beginning for end-of-life plastics" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):107-107,
https://hdl.handle.net/21.15107/rcub_imagine_2144 .

TY  - JOUR
AU  - Milić, Petar
AU  - Rajković, Katarina
AU  - Nikolić, Goran
AU  - Jeremić, Sanja
AU  - Đurašević, Mirjana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0263876223000618
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1922
AB  - The aims of this research were preparation of Juglans nigra husk extracts by employing an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. Extracts from J. nigra L. fresh husk were prepared using ethanol (70%, v/v) as a solvent, at different extraction temperatures: 15, 30 and 40 °C, and extraction times: 5, 15, 30, 60 and 80 min. The content of minerals in ethanol extracts was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The response surface methodology (RSM) was developed to evaluate the effect of two extraction factors as well as to optimize the extraction variables for each mineral individually. The RSM models pointed out 40 °C temperature and extraction time in the range 77–80 min as optimal conditions for extraction of macro- and micro-elements. Results of RSM optimization showed the relative order of maximum obtained yield of macro-elements: K>P>S>Ca>Mg and maximum yield of micro-elements was Si>Zn>Sr>Cu.
T2  - Chemical Engineering Research and Design
T2  - Chemical Engineering Research and DesignChemical Engineering Research and Design
T1  - Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology
EP  - 445
SP  - 439
VL  - 191
DO  - 10.1016/j.cherd.2023.01.051
ER  - 

@article{
author = "Milić, Petar and Rajković, Katarina and Nikolić, Goran and Jeremić, Sanja and Đurašević, Mirjana",
year = "2023",
abstract = "The aims of this research were preparation of Juglans nigra husk extracts by employing an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. Extracts from J. nigra L. fresh husk were prepared using ethanol (70%, v/v) as a solvent, at different extraction temperatures: 15, 30 and 40 °C, and extraction times: 5, 15, 30, 60 and 80 min. The content of minerals in ethanol extracts was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The response surface methodology (RSM) was developed to evaluate the effect of two extraction factors as well as to optimize the extraction variables for each mineral individually. The RSM models pointed out 40 °C temperature and extraction time in the range 77–80 min as optimal conditions for extraction of macro- and micro-elements. Results of RSM optimization showed the relative order of maximum obtained yield of macro-elements: K>P>S>Ca>Mg and maximum yield of micro-elements was Si>Zn>Sr>Cu.",
journal = "Chemical Engineering Research and Design, Chemical Engineering Research and DesignChemical Engineering Research and Design",
title = "Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology",
pages = "445-439",
volume = "191",
doi = "10.1016/j.cherd.2023.01.051"
}

Milić, P., Rajković, K., Nikolić, G., Jeremić, S.,& Đurašević, M.. (2023). Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. in Chemical Engineering Research and Design, 191, 439-445.
https://doi.org/10.1016/j.cherd.2023.01.051

Milić P, Rajković K, Nikolić G, Jeremić S, Đurašević M. Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. in Chemical Engineering Research and Design. 2023;191:439-445.
doi:10.1016/j.cherd.2023.01.051 .

Milić, Petar, Rajković, Katarina, Nikolić, Goran, Jeremić, Sanja, Đurašević, Mirjana, "Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology" in Chemical Engineering Research and Design, 191 (2023):439-445,
https://doi.org/10.1016/j.cherd.2023.01.051 . .

TY  - JOUR
AU  - Milić, Petar
AU  - Rajković, Katarina
AU  - Nikolić, Goran
AU  - Jeremić, Sanja
AU  - Đurašević, Mirjana
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0263876223000618
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1888
AB  - The aims of this research were preparation of Juglans nigra husk extracts by employing an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. Extracts from J. nigra L. fresh husk were prepared using ethanol (70%, v/v) as a solvent, at different extraction temperatures: 15, 30 and 40 °C, and extraction times: 5, 15, 30, 60 and 80 min. The content of minerals in ethanol extracts was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The response surface methodology (RSM) was developed to evaluate the effect of two extraction factors as well as to optimize the extraction variables for each mineral individually. The RSM models pointed out 40 °C temperature and extraction time in the range 77–80 min as optimal conditions for extraction of macro- and micro-elements. Results of RSM optimization showed the relative order of maximum obtained yield of macro-elements: K>P>S>Ca>Mg and maximum yield of micro-elements was Si>Zn>Sr>Cu.
T2  - Chemical Engineering Research and Design
T2  - Chemical Engineering Research and DesignChemical Engineering Research and Design
T1  - Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology
EP  - 445
SP  - 439
VL  - 191
DO  - 10.1016/j.cherd.2023.01.051
ER  - 

@article{
author = "Milić, Petar and Rajković, Katarina and Nikolić, Goran and Jeremić, Sanja and Đurašević, Mirjana",
year = "2023",
abstract = "The aims of this research were preparation of Juglans nigra husk extracts by employing an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. Extracts from J. nigra L. fresh husk were prepared using ethanol (70%, v/v) as a solvent, at different extraction temperatures: 15, 30 and 40 °C, and extraction times: 5, 15, 30, 60 and 80 min. The content of minerals in ethanol extracts was determined using inductively coupled plasma optical emission spectrometry (ICP-OES). The response surface methodology (RSM) was developed to evaluate the effect of two extraction factors as well as to optimize the extraction variables for each mineral individually. The RSM models pointed out 40 °C temperature and extraction time in the range 77–80 min as optimal conditions for extraction of macro- and micro-elements. Results of RSM optimization showed the relative order of maximum obtained yield of macro-elements: K>P>S>Ca>Mg and maximum yield of micro-elements was Si>Zn>Sr>Cu.",
journal = "Chemical Engineering Research and Design, Chemical Engineering Research and DesignChemical Engineering Research and Design",
title = "Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology",
pages = "445-439",
volume = "191",
doi = "10.1016/j.cherd.2023.01.051"
}

Milić, P., Rajković, K., Nikolić, G., Jeremić, S.,& Đurašević, M.. (2023). Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. in Chemical Engineering Research and Design, 191, 439-445.
https://doi.org/10.1016/j.cherd.2023.01.051

Milić P, Rajković K, Nikolić G, Jeremić S, Đurašević M. Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology. in Chemical Engineering Research and Design. 2023;191:439-445.
doi:10.1016/j.cherd.2023.01.051 .

Milić, Petar, Rajković, Katarina, Nikolić, Goran, Jeremić, Sanja, Đurašević, Mirjana, "Preparation of Juglans nigra L. husk extracts by applying an ultrasonic-assisted extraction method and process optimization of minerals extraction using response surface methodology" in Chemical Engineering Research and Design, 191 (2023):439-445,
https://doi.org/10.1016/j.cherd.2023.01.051 . .

TY  - JOUR
AU  - Rajković, Katarina
AU  - Drobac, Milica
AU  - Milić, Petar
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Jeremić, Sanja
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1805
AB  - Juglans nigra (Black walnut) is a source of health-promoting
biologically active compounds used in traditional medicine. The investigation of
bioactive compounds in black walnut could lead to its broader application, as
well as application of its by-products. Therefore, this study aimed to characterize
J. nigra nut and green husk based on chemical analysis of their petroleum ether
and ethanol extracts obtained by ultrasonic and reflux extraction methods,
respectively. Different extract fractions were tested for their antimicrobial
activities using Gram-negative bacteria (Escherichia coli, Pseudomonas
aeruginosa), Gram-positive bacteria (Enterococcus faecalis Staphylococcus
aureus) and yeast (reference strain and clinical isolates of Candida albicans).
Ethanol extracts analysis, performed by High Performance Liquid
Chromatography (HPLC), singled out the ellagic acid as the most dominant
compound in nut (55.0 ± 1.3 × 10-3 kg m-3
) and green husk (114.1 ± 0.5× 10-3 kg
m-3
) extracts. Non-polar compounds were evaluated using Gas Chromatography
(GC) analysis of petroleum ether extracts. Juglans nigra nut and green husk
contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid
(C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic
(C18:1n-9), and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids,
linoleic (C18:2n-6), γ-linolenic (C18:3n-6), and α-linolenic (C18:3n-3) acids.
Ethanol extracts of both J. nigra nut and green husk showed antimicrobial
activity against C. albicans which is the most common cause of yeast infections.
AB  - Juglans nigra (црни орах) као извор биолошки активних једињења користе се у
традиционалној медицини. Истраживање биоактивних једињења присутних у црном ораху
довело би до шире примене његових производа. Зато ова студија има за циљ да
окарактерише орашаст плод и зелену љуску Ј. nigra на основу хемијске анализе њихових
петролетарских и етанолних екстраката, добијених ултразвучним и рефлукс методама
екстракције. Антимикробна активност различитих фракција екстракта је тестирана
коришћењем Грам-негативних бактерија (Escherichia coli, Pseudomonas aeruginosa), Грамозитивних бактерија (Enterococcus faecalis Staphylococcus aureus) и квасница (референтни
сој и клинички изолати Candida albicans). Анализа течном хроматографијом високих
перформанси, издвојила је елагинску киселину као најдоминантније једињење у етанолним
екстрактима орашастог плода (55,0 ± 1,3 × 10-3 kg m-3) и зелене љуске (114,1 ± 0,5 × 10-3
g m-3). Неполарна једињења су процењена применом гасне хроматографске анализе
петролетарских екстракта. Орашаст плод и зелена љуска садрже две засићене масне
киселине, палмитинску (C16:0) и стеаринску киселину (C18:0), затим мононезасићене
масне киселине, палмитолеинску (C16:1n-7), олеинску (C18:1n-9), и вакценску киселину
(C18:1n-7), као и полинезасићене масне киселине, линолну (C18:2n-6), γ-линоленску
(C18:3n-6) и α-линоленску (C18: 3n-3) киселину. Етанолни екстракти орашастог плода и
зелене љуске показали су антимикробну активност нa сојевима C. albicans која је најчешћи
узрочник гљивичних инфекција.
PB  - Belgrade : Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
T1  - Hemijska karakterizacija i antimikrobna aktivnost orašastog ploda i zelene ljuske Juglans nigra l.
T1  - Xемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra l.
DO  - doi.org/10.2298/JSC230210024R
ER  - 

@article{
author = "Rajković, Katarina and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirjana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena",
year = "2023",
abstract = "Juglans nigra (Black walnut) is a source of health-promoting
biologically active compounds used in traditional medicine. The investigation of
bioactive compounds in black walnut could lead to its broader application, as
well as application of its by-products. Therefore, this study aimed to characterize
J. nigra nut and green husk based on chemical analysis of their petroleum ether
and ethanol extracts obtained by ultrasonic and reflux extraction methods,
respectively. Different extract fractions were tested for their antimicrobial
activities using Gram-negative bacteria (Escherichia coli, Pseudomonas
aeruginosa), Gram-positive bacteria (Enterococcus faecalis Staphylococcus
aureus) and yeast (reference strain and clinical isolates of Candida albicans).
Ethanol extracts analysis, performed by High Performance Liquid
Chromatography (HPLC), singled out the ellagic acid as the most dominant
compound in nut (55.0 ± 1.3 × 10-3 kg m-3
) and green husk (114.1 ± 0.5× 10-3 kg
m-3
) extracts. Non-polar compounds were evaluated using Gas Chromatography
(GC) analysis of petroleum ether extracts. Juglans nigra nut and green husk
contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid
(C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic
(C18:1n-9), and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids,
linoleic (C18:2n-6), γ-linolenic (C18:3n-6), and α-linolenic (C18:3n-3) acids.
Ethanol extracts of both J. nigra nut and green husk showed antimicrobial
activity against C. albicans which is the most common cause of yeast infections., Juglans nigra (црни орах) као извор биолошки активних једињења користе се у
традиционалној медицини. Истраживање биоактивних једињења присутних у црном ораху
довело би до шире примене његових производа. Зато ова студија има за циљ да
окарактерише орашаст плод и зелену љуску Ј. nigra на основу хемијске анализе њихових
петролетарских и етанолних екстраката, добијених ултразвучним и рефлукс методама
екстракције. Антимикробна активност различитих фракција екстракта је тестирана
коришћењем Грам-негативних бактерија (Escherichia coli, Pseudomonas aeruginosa), Грамозитивних бактерија (Enterococcus faecalis Staphylococcus aureus) и квасница (референтни
сој и клинички изолати Candida albicans). Анализа течном хроматографијом високих
перформанси, издвојила је елагинску киселину као најдоминантније једињење у етанолним
екстрактима орашастог плода (55,0 ± 1,3 × 10-3 kg m-3) и зелене љуске (114,1 ± 0,5 × 10-3
g m-3). Неполарна једињења су процењена применом гасне хроматографске анализе
петролетарских екстракта. Орашаст плод и зелена љуска садрже две засићене масне
киселине, палмитинску (C16:0) и стеаринску киселину (C18:0), затим мононезасићене
масне киселине, палмитолеинску (C16:1n-7), олеинску (C18:1n-9), и вакценску киселину
(C18:1n-7), као и полинезасићене масне киселине, линолну (C18:2n-6), γ-линоленску
(C18:3n-6) и α-линоленску (C18: 3n-3) киселину. Етанолни екстракти орашастог плода и
зелене љуске показали су антимикробну активност нa сојевима C. albicans која је најчешћи
узрочник гљивичних инфекција.",
publisher = "Belgrade : Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk, Hemijska karakterizacija i antimikrobna aktivnost orašastog ploda i zelene ljuske Juglans nigra l., Xемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra l.",
doi = "doi.org/10.2298/JSC230210024R"
}

Rajković, K., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society
Belgrade : Serbian Chemical Society..
https://doi.org/doi.org/10.2298/JSC230210024R

Rajković K, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;.
doi:doi.org/10.2298/JSC230210024R .

Rajković, Katarina, Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirjana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society (2023),
https://doi.org/doi.org/10.2298/JSC230210024R . .

TY  - JOUR
AU  - Sourkouni, Georgia
AU  - Jeremić, Sanja
AU  - Kalogirou, Charalampia
AU  - Höfft, Oliver
AU  - Nenadović, Marija
AU  - Janković, Vukašin
AU  - Rajasekaran, Divya
AU  - Pandis, Pavlos
AU  - Padamati, Ramesh
AU  - Nikodinović-Runić, Jasmina
AU  - Argirusis, Christos
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1804
AB  - It is well acknowledged that microplastics are a major environmental problem and that the use of plastics, both petro- and bio- based, should be reduced. Nevertheless, it is also a necessity to reduce the amount of the already spread plastics. These cannot be easily degraded in the nature and accumulate in the food supply chain with major danger for animals and human life. It has been shown in the literature that advanced oxidation processes (AOPs) modify the surface of polylactic acid (PLA) materials in a way that bacteria more efficiently dock on their surface and eventually degrade them. In the present work we investigated the influence of different AOPs (ultrasounds, ultraviolet irradiation, and their combination) on the biodegradability of PLA films treated for different times between 1 and 6 h. The pre-treated samples have been degraded using a home model compost as well as a cocktail of commercial enzymes at mesophilic temperatures (37 °C and 42 °C, respectively). Degradation degree has been measured and degradation products have been identified. Excellent degradation of PLA films has been achieved with enzyme cocktail containing commercial alkaline proteases and lipases of up to 90% weight loss. For the first time, we also report valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.
T2  - World Journal of Microbiology and Biotechnology
T2  - World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol
T1  - Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose
IS  - 6
SP  - 161
VL  - 39
DO  - 10.1007/s11274-023-03605-4
ER  - 

@article{
author = "Sourkouni, Georgia and Jeremić, Sanja and Kalogirou, Charalampia and Höfft, Oliver and Nenadović, Marija and Janković, Vukašin and Rajasekaran, Divya and Pandis, Pavlos and Padamati, Ramesh and Nikodinović-Runić, Jasmina and Argirusis, Christos",
year = "2023",
abstract = "It is well acknowledged that microplastics are a major environmental problem and that the use of plastics, both petro- and bio- based, should be reduced. Nevertheless, it is also a necessity to reduce the amount of the already spread plastics. These cannot be easily degraded in the nature and accumulate in the food supply chain with major danger for animals and human life. It has been shown in the literature that advanced oxidation processes (AOPs) modify the surface of polylactic acid (PLA) materials in a way that bacteria more efficiently dock on their surface and eventually degrade them. In the present work we investigated the influence of different AOPs (ultrasounds, ultraviolet irradiation, and their combination) on the biodegradability of PLA films treated for different times between 1 and 6 h. The pre-treated samples have been degraded using a home model compost as well as a cocktail of commercial enzymes at mesophilic temperatures (37 °C and 42 °C, respectively). Degradation degree has been measured and degradation products have been identified. Excellent degradation of PLA films has been achieved with enzyme cocktail containing commercial alkaline proteases and lipases of up to 90% weight loss. For the first time, we also report valorization of PLA into bacterial nanocellulose after enzymatic hydrolysis of the samples.",
journal = "World Journal of Microbiology and Biotechnology, World Journal of Microbiology and BiotechnologyWorld J Microbiol Biotechnol",
title = "Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose",
number = "6",
pages = "161",
volume = "39",
doi = "10.1007/s11274-023-03605-4"
}

Sourkouni, G., Jeremić, S., Kalogirou, C., Höfft, O., Nenadović, M., Janković, V., Rajasekaran, D., Pandis, P., Padamati, R., Nikodinović-Runić, J.,& Argirusis, C.. (2023). Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose. in World Journal of Microbiology and Biotechnology, 39(6), 161.
https://doi.org/10.1007/s11274-023-03605-4

Sourkouni G, Jeremić S, Kalogirou C, Höfft O, Nenadović M, Janković V, Rajasekaran D, Pandis P, Padamati R, Nikodinović-Runić J, Argirusis C. Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose. in World Journal of Microbiology and Biotechnology. 2023;39(6):161.
doi:10.1007/s11274-023-03605-4 .

Sourkouni, Georgia, Jeremić, Sanja, Kalogirou, Charalampia, Höfft, Oliver, Nenadović, Marija, Janković, Vukašin, Rajasekaran, Divya, Pandis, Pavlos, Padamati, Ramesh, Nikodinović-Runić, Jasmina, Argirusis, Christos, "Study of PLA pre-treatment, enzymatic and model-compost degradation, and valorization of degradation products to bacterial nanocellulose" in World Journal of Microbiology and Biotechnology, 39, no. 6 (2023):161,
https://doi.org/10.1007/s11274-023-03605-4 . .

TY  - JOUR
AU  - Salević-Jelić, Ana
AU  - Lević, Steva
AU  - Stojanović, Dušica
AU  - Jeremić, Sanja
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Ignjatović, Ivana Sredović
AU  - Uskoković, Petar
AU  - Nedović, Viktor
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2214289423000042
UR  - https://doi.org/10.1016/j.fpsl.2023.101027
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1921
AB  - This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).
T2  - Food Packaging and Shelf Life
T2  - Food Packaging and Shelf LifeFood Packaging and Shelf Life
T1  - Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization
SP  - 101027
VL  - 35
DO  - 10.1016/j.fpsl.2023.101027
ER  - 

@article{
author = "Salević-Jelić, Ana and Lević, Steva and Stojanović, Dušica and Jeremić, Sanja and Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Ignjatović, Ivana Sredović and Uskoković, Petar and Nedović, Viktor",
year = "2023",
abstract = "This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).",
journal = "Food Packaging and Shelf Life, Food Packaging and Shelf LifeFood Packaging and Shelf Life",
title = "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization",
pages = "101027",
volume = "35",
doi = "10.1016/j.fpsl.2023.101027"
}

Salević-Jelić, A., Lević, S., Stojanović, D., Jeremić, S., Miletić, D., Pantić, M., Pavlović, V., Ignjatović, I. S., Uskoković, P.,& Nedović, V.. (2023). Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life, 35, 101027.
https://doi.org/10.1016/j.fpsl.2023.101027

Salević-Jelić A, Lević S, Stojanović D, Jeremić S, Miletić D, Pantić M, Pavlović V, Ignjatović IS, Uskoković P, Nedović V. Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life. 2023;35:101027.
doi:10.1016/j.fpsl.2023.101027 .

Salević-Jelić, Ana, Lević, Steva, Stojanović, Dušica, Jeremić, Sanja, Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Ignjatović, Ivana Sredović, Uskoković, Petar, Nedović, Viktor, "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization" in Food Packaging and Shelf Life, 35 (2023):101027,
https://doi.org/10.1016/j.fpsl.2023.101027 . .

TY  - JOUR
AU  - Salević-Jelić, Ana
AU  - Lević, Steva
AU  - Stojanović, Dušica
AU  - Jeremić, Sanja
AU  - Miletić, Dunja
AU  - Pantić, Milena
AU  - Pavlović, Vladimir
AU  - Ignjatović, Ivana Sredović
AU  - Uskoković, Petar
AU  - Nedović, Viktor
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2214289423000042
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1887
UR  - https://doi.org/10.1016/j.fpsl.2023.101027
AB  - This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).
T2  - Food Packaging and Shelf Life
T2  - Food Packaging and Shelf LifeFood Packaging and Shelf Life
T1  - Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization
SP  - 101027
VL  - 35
DO  - 10.1016/j.fpsl.2023.101027
ER  - 

@article{
author = "Salević-Jelić, Ana and Lević, Steva and Stojanović, Dušica and Jeremić, Sanja and Miletić, Dunja and Pantić, Milena and Pavlović, Vladimir and Ignjatović, Ivana Sredović and Uskoković, Petar and Nedović, Viktor",
year = "2023",
abstract = "This study aimed to develop active, biodegradable materials for food packaging by incorporating sage extract (SE) within a zein-gelatin blend by electrospinning and solvent casting. The fabrication techniques, SE incorporation, and its content (5, 10% w/w) determined the materials’ properties. Electrospinning produced 0.36–0.53 mm thick, non-transparent fibrous mats (mean fiber diameter 1.12–1.36 µm). Solvent casting generated 0.34–0.41 mm thick, transparent continuous films. The analysis indicated the constituents’ compatibility, homogenous dispersion, and efficient SE incorporation without strong chemical interactions and phase separation. The solvent-cast films presented more ordered structures, higher mechanical resistance, elongation, and water vapor barrier performance than the electrospun mats. The SE-incorporating formulations showed phenolics’ delivery ability to food simulants influenced by structure, SE content, and media polarity. The electrospun mats expressed higher DPPH• radicals’ inhibition, while the solvent-cast films showed stronger Staphylococcus aureus and Escherichia coli growth inhibition, increased by SE incorporation. All formulations showed rapid complete bio-disintegration in compost (18–25 days).",
journal = "Food Packaging and Shelf Life, Food Packaging and Shelf LifeFood Packaging and Shelf Life",
title = "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization",
pages = "101027",
volume = "35",
doi = "10.1016/j.fpsl.2023.101027"
}

Salević-Jelić, A., Lević, S., Stojanović, D., Jeremić, S., Miletić, D., Pantić, M., Pavlović, V., Ignjatović, I. S., Uskoković, P.,& Nedović, V.. (2023). Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life, 35, 101027.
https://doi.org/10.1016/j.fpsl.2023.101027

Salević-Jelić A, Lević S, Stojanović D, Jeremić S, Miletić D, Pantić M, Pavlović V, Ignjatović IS, Uskoković P, Nedović V. Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization. in Food Packaging and Shelf Life. 2023;35:101027.
doi:10.1016/j.fpsl.2023.101027 .

Salević-Jelić, Ana, Lević, Steva, Stojanović, Dušica, Jeremić, Sanja, Miletić, Dunja, Pantić, Milena, Pavlović, Vladimir, Ignjatović, Ivana Sredović, Uskoković, Petar, Nedović, Viktor, "Biodegradable and active zein-gelatin-based electrospun mats and solvent-cast films incorporating sage extract: Formulation and comparative characterization" in Food Packaging and Shelf Life, 35 (2023):101027,
https://doi.org/10.1016/j.fpsl.2023.101027 . .

TY  - CONF
AU  - Jeremić, Sanja
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1927
AB  - Iako je već čitav vek prisutna u životima ljudi, plastika je i dalje jedan od
najraznovrsnijih, najčešće proizvođenih i korišćenih materijala. Nekada najveća prednost
plastike – izdržljivost – danas predstavlja veliki problem, jer je čini teško razgradivim
materijalom koji se gomila u životnoj sredini [1]. Najčešće korišćen pristup za odlaganje
ovog polimera je deponovanje, koje je pored ekološke pretnje ujedno i ekonomski izazov,
jer se ovakvim odlaganjem plastike gubi uložena energija i mogućnost za ponovnu upotrebu
materijala. Sa druge strane, biološki proces, zasnovan na enzimskoj razgradnji, pruža
nekoliko prednosti: blage uslove, nizak utrošak energije, i odsustvo opasnih hemikalija [2].
Poseban značaj enzimske razgradnje plastike je što obezbeđuje niz metabolita - polaznih
jedinjenja za proizvodnju novih vrednih polimera [3], čime se doprinosi uspostavljanju
cirkularne ekonomije kada su u pitanju plastični materijali.
Paralelno sa razvijanjem i unapređivanjem bioloških pristupa u razgradnji plastičnog
otpada, istražuju se i ekološki prihvatljivi materijali koji bi mogli zameniti plastiku, kao što
je biorazgradiv biopolimer - bakterijska nanoceluloza (Slika 1). Zahvaljujući svojim
izvanrednim svojstvima kao što su mehanička čvrstoća, hidrofilnost, biokompatibilnost,
obnovljivost i netoksičnost, bakterijska nanocelloza ima potencijal za primenu u različitim
granama industrije [4]. Međutim, produkcija bakterijske nanoceluloze na industrijskoj skali
je otežana visokom cenom medijuma za rast bakterija proizvođača. Iz tog razloga su svetska
istraživanja poslednjih godina usmerena na optimizaciju produkcije bakterijske
nanoceluloze korišćenjem različitih vrsta otpada [5].
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Na raskršću puteva razgradnje plastike i produkcije biomaterijala
T1  - Crossing roads of plastic degradation and biomaterial production
EP  - 26
SP  - 25
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1927
ER  - 

@conference{
author = "Jeremić, Sanja",
year = "2023",
abstract = "Iako je već čitav vek prisutna u životima ljudi, plastika je i dalje jedan od
najraznovrsnijih, najčešće proizvođenih i korišćenih materijala. Nekada najveća prednost
plastike – izdržljivost – danas predstavlja veliki problem, jer je čini teško razgradivim
materijalom koji se gomila u životnoj sredini [1]. Najčešće korišćen pristup za odlaganje
ovog polimera je deponovanje, koje je pored ekološke pretnje ujedno i ekonomski izazov,
jer se ovakvim odlaganjem plastike gubi uložena energija i mogućnost za ponovnu upotrebu
materijala. Sa druge strane, biološki proces, zasnovan na enzimskoj razgradnji, pruža
nekoliko prednosti: blage uslove, nizak utrošak energije, i odsustvo opasnih hemikalija [2].
Poseban značaj enzimske razgradnje plastike je što obezbeđuje niz metabolita - polaznih
jedinjenja za proizvodnju novih vrednih polimera [3], čime se doprinosi uspostavljanju
cirkularne ekonomije kada su u pitanju plastični materijali.
Paralelno sa razvijanjem i unapređivanjem bioloških pristupa u razgradnji plastičnog
otpada, istražuju se i ekološki prihvatljivi materijali koji bi mogli zameniti plastiku, kao što
je biorazgradiv biopolimer - bakterijska nanoceluloza (Slika 1). Zahvaljujući svojim
izvanrednim svojstvima kao što su mehanička čvrstoća, hidrofilnost, biokompatibilnost,
obnovljivost i netoksičnost, bakterijska nanocelloza ima potencijal za primenu u različitim
granama industrije [4]. Međutim, produkcija bakterijske nanoceluloze na industrijskoj skali
je otežana visokom cenom medijuma za rast bakterija proizvođača. Iz tog razloga su svetska
istraživanja poslednjih godina usmerena na optimizaciju produkcije bakterijske
nanoceluloze korišćenjem različitih vrsta otpada [5].",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Na raskršću puteva razgradnje plastike i produkcije biomaterijala, Crossing roads of plastic degradation and biomaterial production",
pages = "26-25",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1927"
}

Jeremić, S.. (2023). Na raskršću puteva razgradnje plastike i produkcije biomaterijala. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 25-26.
https://hdl.handle.net/21.15107/rcub_imagine_1927

Jeremić S. Na raskršću puteva razgradnje plastike i produkcije biomaterijala. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:25-26.
https://hdl.handle.net/21.15107/rcub_imagine_1927 .

Jeremić, Sanja, "Na raskršću puteva razgradnje plastike i produkcije biomaterijala" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):25-26,
https://hdl.handle.net/21.15107/rcub_imagine_1927 .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Stevanovic, Sanja
AU  - Nikodinović-Runić, Jasmina
AU  - Jeremić, Sanja
AU  - Cosovic, Vladan R.
AU  - Maksimovic, Vesna
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1659
AB  - Bacterial nanocellulose, BNC, has emerged as a new class of nanomaterials recognized as renewable, biodegradable, biocompatible and material for versatile applications. BNC also proved as a perfect support matrix for metallic nanoparticle synthesis and appeared as suitable alternative for widely used carbon based materials. Following the idea to replace commonly used carbon based materials for platinum supports with the green and sustainable one, BNC appeared as an excellent candidate. Herein, microwave assisted synthesis has been reported for the first time for platinum nanoparticles supported on BNC as green material. Bacterial nanocelullose-platinum catalyst, Pt/BNC, was investigated by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), atomic force microscopy (AFM), X-ray diffractometry (XRD) and transmission-electron microscopy (TEM) analysis. The obtained results confirmed successful synthesis of new Pt-based catalyst. It was found that Pt/BNC catalyst has high electrocatalytic performance in methanol oxidation reaction. Green/sustainable catalytic system is highly desirable and provided by the elegant microwave assisted synthesis of Pt/BNC will pave the way for a larger scale application and expedite the market penetration of such fuel cells.
T2  - International Journal of Biological Macromolecules
T2  - International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules
T1  - Bacterial nanocellulose as green support of platinum nanoparticles for effective methanol oxidation
EP  - 1484
SP  - 1474
VL  - 223
DO  - 10.1016/j.ijbiomac.2022.10.278
ER  - 

@article{
author = "Ponjavić, Marijana and Stevanovic, Sanja and Nikodinović-Runić, Jasmina and Jeremić, Sanja and Cosovic, Vladan R. and Maksimovic, Vesna",
year = "2022",
abstract = "Bacterial nanocellulose, BNC, has emerged as a new class of nanomaterials recognized as renewable, biodegradable, biocompatible and material for versatile applications. BNC also proved as a perfect support matrix for metallic nanoparticle synthesis and appeared as suitable alternative for widely used carbon based materials. Following the idea to replace commonly used carbon based materials for platinum supports with the green and sustainable one, BNC appeared as an excellent candidate. Herein, microwave assisted synthesis has been reported for the first time for platinum nanoparticles supported on BNC as green material. Bacterial nanocelullose-platinum catalyst, Pt/BNC, was investigated by Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), atomic force microscopy (AFM), X-ray diffractometry (XRD) and transmission-electron microscopy (TEM) analysis. The obtained results confirmed successful synthesis of new Pt-based catalyst. It was found that Pt/BNC catalyst has high electrocatalytic performance in methanol oxidation reaction. Green/sustainable catalytic system is highly desirable and provided by the elegant microwave assisted synthesis of Pt/BNC will pave the way for a larger scale application and expedite the market penetration of such fuel cells.",
journal = "International Journal of Biological Macromolecules, International Journal of Biological MacromoleculesInternational Journal of Biological Macromolecules",
title = "Bacterial nanocellulose as green support of platinum nanoparticles for effective methanol oxidation",
pages = "1484-1474",
volume = "223",
doi = "10.1016/j.ijbiomac.2022.10.278"
}

Ponjavić, M., Stevanovic, S., Nikodinović-Runić, J., Jeremić, S., Cosovic, V. R.,& Maksimovic, V.. (2022). Bacterial nanocellulose as green support of platinum nanoparticles for effective methanol oxidation. in International Journal of Biological Macromolecules, 223, 1474-1484.
https://doi.org/10.1016/j.ijbiomac.2022.10.278

Ponjavić M, Stevanovic S, Nikodinović-Runić J, Jeremić S, Cosovic VR, Maksimovic V. Bacterial nanocellulose as green support of platinum nanoparticles for effective methanol oxidation. in International Journal of Biological Macromolecules. 2022;223:1474-1484.
doi:10.1016/j.ijbiomac.2022.10.278 .

Ponjavić, Marijana, Stevanovic, Sanja, Nikodinović-Runić, Jasmina, Jeremić, Sanja, Cosovic, Vladan R., Maksimovic, Vesna, "Bacterial nanocellulose as green support of platinum nanoparticles for effective methanol oxidation" in International Journal of Biological Macromolecules, 223 (2022):1474-1484,
https://doi.org/10.1016/j.ijbiomac.2022.10.278 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Kováčová, Mária
AU  - Jeremić, Sanja 
AU  - Nagy, Štefan
AU  - Milivojević, Dušan D.
AU  - Kubat, Pavel
AU  - Kleinová, Angela
AU  - Budimir, Milica D.
AU  - Mojsin, Marija
AU  - Stevanović, Milena 
AU  - Annušová, Adriana
AU  - Špitalský, Zdeno
AU  - Todorović Marković, Biljana M.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1667
AB  - Development of new types of antimicrobial coatings is of utmost importance due to increasing problems with pathogen transmission from various infectious surfaces to human beings. In this study, new types of highly potent antimicrobial polyurethane composite films encapsulated by hydrophobic riboflavin-based carbon polymer dots are presented. Detailed structural, optical, antimicrobial, and cytotoxic investigations of these composites were conducted. Low-power blue light triggered the composites to eradicate Escherichia coli in 30 min, whereas the same effect toward Staphylococcus aureus was reached after 60 min. These composites also show low toxicity against MRC-5 cells. In this way, RF-CPD composites can be used for sterilization of highly touched objects in the healthcare industry.
T2  - Nanomaterials
T2  - Nanomaterials
T1  - Highly Efficient Antibacterial Polymer Composites Based on Hydrophobic Riboflavin Carbon Polymerized Dots
IS  - 22
SP  - 4070
VL  - 12
DO  - 10.3390/nano12224070
ER  - 

@article{
author = "Marković, Zoran M. and Kováčová, Mária and Jeremić, Sanja  and Nagy, Štefan and Milivojević, Dušan D. and Kubat, Pavel and Kleinová, Angela and Budimir, Milica D. and Mojsin, Marija and Stevanović, Milena  and Annušová, Adriana and Špitalský, Zdeno and Todorović Marković, Biljana M.",
year = "2022",
abstract = "Development of new types of antimicrobial coatings is of utmost importance due to increasing problems with pathogen transmission from various infectious surfaces to human beings. In this study, new types of highly potent antimicrobial polyurethane composite films encapsulated by hydrophobic riboflavin-based carbon polymer dots are presented. Detailed structural, optical, antimicrobial, and cytotoxic investigations of these composites were conducted. Low-power blue light triggered the composites to eradicate Escherichia coli in 30 min, whereas the same effect toward Staphylococcus aureus was reached after 60 min. These composites also show low toxicity against MRC-5 cells. In this way, RF-CPD composites can be used for sterilization of highly touched objects in the healthcare industry.",
journal = "Nanomaterials, Nanomaterials",
title = "Highly Efficient Antibacterial Polymer Composites Based on Hydrophobic Riboflavin Carbon Polymerized Dots",
number = "22",
pages = "4070",
volume = "12",
doi = "10.3390/nano12224070"
}

Marković, Z. M., Kováčová, M., Jeremić, S., Nagy, Š., Milivojević, D. D., Kubat, P., Kleinová, A., Budimir, M. D., Mojsin, M., Stevanović, M., Annušová, A., Špitalský, Z.,& Todorović Marković, B. M.. (2022). Highly Efficient Antibacterial Polymer Composites Based on Hydrophobic Riboflavin Carbon Polymerized Dots. in Nanomaterials, 12(22), 4070.
https://doi.org/10.3390/nano12224070

Marković ZM, Kováčová M, Jeremić S, Nagy Š, Milivojević DD, Kubat P, Kleinová A, Budimir MD, Mojsin M, Stevanović M, Annušová A, Špitalský Z, Todorović Marković BM. Highly Efficient Antibacterial Polymer Composites Based on Hydrophobic Riboflavin Carbon Polymerized Dots. in Nanomaterials. 2022;12(22):4070.
doi:10.3390/nano12224070 .

Marković, Zoran M., Kováčová, Mária, Jeremić, Sanja , Nagy, Štefan, Milivojević, Dušan D., Kubat, Pavel, Kleinová, Angela, Budimir, Milica D., Mojsin, Marija, Stevanović, Milena , Annušová, Adriana, Špitalský, Zdeno, Todorović Marković, Biljana M., "Highly Efficient Antibacterial Polymer Composites Based on Hydrophobic Riboflavin Carbon Polymerized Dots" in Nanomaterials, 12, no. 22 (2022):4070,
https://doi.org/10.3390/nano12224070 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Nikolić, Marija S.
AU  - Jevtić, Sanja
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Donlagić, Jasna
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1580
AB  - The present study reports the potential application of star-shaped poly(epsilon-caprolactones) with different number of arms as new drug delivery matrix. Linear and star-shaped PCL ibuprofen loaded microspheres were prepared using oil-in-water (o/w) solvent evaporation technique and characterized with FTIR, DSC, XRD and SEM analysis. High yield, encapsulation efficiency and drug loadings were obtained for all microspheres. FTIR analysis revealed the existence of interactions between polymer matrix and drug, while the DSC analysis suggested that drug was encapsulated in an amorphous form. SEM analysis confirmed that regular, spherical in shape star-shaped microspheres, with diameter between 80 and 90 mu n, were obtained, while quite larger microspheres, 110 mu m, were prepared from linear PCL. The advantage of using starshaped PCL microspheres instead of linear PCL was seen from drug release profiles which demonstrated higher amount of drug released from star-shaped polymer matrix as a consequence of their branched, flexible structure. Microspheres prepared from the polymers with the most branched structure showed the highest amount of the released drug after 24 h. Finally, cytotoxicity tests, performed using normal human fibroblasts (MRCS), indicated the absence of cytotoxicity at lower concentrations of microspheres proving the great potential of star-shaped PCL systems in comparison to linear ones.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Star-shaped poly(epsilon-caprolactones) with well-defined architecture as potential drug carriers
DO  - 10.2298/JSC220202032P
ER  - 

@article{
author = "Ponjavić, Marijana and Nikolić, Marija S. and Jevtić, Sanja and Jeremić, Sanja and Đokić, Lidija and Donlagić, Jasna",
year = "2022",
abstract = "The present study reports the potential application of star-shaped poly(epsilon-caprolactones) with different number of arms as new drug delivery matrix. Linear and star-shaped PCL ibuprofen loaded microspheres were prepared using oil-in-water (o/w) solvent evaporation technique and characterized with FTIR, DSC, XRD and SEM analysis. High yield, encapsulation efficiency and drug loadings were obtained for all microspheres. FTIR analysis revealed the existence of interactions between polymer matrix and drug, while the DSC analysis suggested that drug was encapsulated in an amorphous form. SEM analysis confirmed that regular, spherical in shape star-shaped microspheres, with diameter between 80 and 90 mu n, were obtained, while quite larger microspheres, 110 mu m, were prepared from linear PCL. The advantage of using starshaped PCL microspheres instead of linear PCL was seen from drug release profiles which demonstrated higher amount of drug released from star-shaped polymer matrix as a consequence of their branched, flexible structure. Microspheres prepared from the polymers with the most branched structure showed the highest amount of the released drug after 24 h. Finally, cytotoxicity tests, performed using normal human fibroblasts (MRCS), indicated the absence of cytotoxicity at lower concentrations of microspheres proving the great potential of star-shaped PCL systems in comparison to linear ones.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Star-shaped poly(epsilon-caprolactones) with well-defined architecture as potential drug carriers",
doi = "10.2298/JSC220202032P"
}

Ponjavić, M., Nikolić, M. S., Jevtić, S., Jeremić, S., Đokić, L.,& Donlagić, J.. (2022). Star-shaped poly(epsilon-caprolactones) with well-defined architecture as potential drug carriers. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd..
https://doi.org/10.2298/JSC220202032P

Ponjavić M, Nikolić MS, Jevtić S, Jeremić S, Đokić L, Donlagić J. Star-shaped poly(epsilon-caprolactones) with well-defined architecture as potential drug carriers. in Journal of the Serbian Chemical Society. 2022;.
doi:10.2298/JSC220202032P .

Ponjavić, Marijana, Nikolić, Marija S., Jevtić, Sanja, Jeremić, Sanja, Đokić, Lidija, Donlagić, Jasna, "Star-shaped poly(epsilon-caprolactones) with well-defined architecture as potential drug carriers" in Journal of the Serbian Chemical Society (2022),
https://doi.org/10.2298/JSC220202032P . .

TY  - JOUR
AU  - Assaleh, Mohamed H.
AU  - Jeremić, Sanja
AU  - Cvijeti, Ilija
AU  - Marinkovi, Aleksandar
AU  - Prlainovi, Nevena
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1601
AB  - Antimicrobial-resistance (AMR) has become the greatest concern and highly challenging issue when treating nosocomial infections. The exigency to develop new potent compounds continues to increase worldwide, whereby derivatives of natural products are becoming more attractive. In the present pa-per, the microbiological assessment of a series of 12 cinnamide hydrazides, four of them completely novel, against clinically relevant pathogens has discovered several derivatives with promising in vitro activities against Acinetobacter baumannii, one of the most dreaded opportunistic pathogens in hospi-tals. The compounds were synthesized by combining one of three different natural acids (cinnamic, 4-chloro or 4-methoxy) with four monothiocarbohydrazones (MTCHs) -an important class of synthetic organic molecules. Their structure was confirmed by elemental microanalysis, as well as ATR-FTIR, H-1 and C-13 NMR spectra, with the addition of 2D NMR spectra for novel compounds. The hybrids of cinnamic acids and pyridine derivatives are particularly active compounds with the lowest MIC50 value of 10.4 mu M for p-chloro cinnamic acid and acetyl pyridine derivatives. An alignment-independent 3D QSAR model identified pharmacophoric hotspots and suggested several structural modifications that might improve the potency of this class of compounds against A. baumannii. The compounds are strong iron-chelating agents forming complexes with a stability constant between 10 7 and 10 9 . The synthesized derivatives represent a promising class of antibacterial compounds with activities comparable to the commonly used antibiotics.
PB  - Elsevier, Amsterdam
T2  - Journal of Molecular Structure
T1  - In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens
VL  - 1262
DO  - 10.1016/j.molstruc.2022.133016
ER  - 

@article{
author = "Assaleh, Mohamed H. and Jeremić, Sanja and Cvijeti, Ilija and Marinkovi, Aleksandar and Prlainovi, Nevena",
year = "2022",
abstract = "Antimicrobial-resistance (AMR) has become the greatest concern and highly challenging issue when treating nosocomial infections. The exigency to develop new potent compounds continues to increase worldwide, whereby derivatives of natural products are becoming more attractive. In the present pa-per, the microbiological assessment of a series of 12 cinnamide hydrazides, four of them completely novel, against clinically relevant pathogens has discovered several derivatives with promising in vitro activities against Acinetobacter baumannii, one of the most dreaded opportunistic pathogens in hospi-tals. The compounds were synthesized by combining one of three different natural acids (cinnamic, 4-chloro or 4-methoxy) with four monothiocarbohydrazones (MTCHs) -an important class of synthetic organic molecules. Their structure was confirmed by elemental microanalysis, as well as ATR-FTIR, H-1 and C-13 NMR spectra, with the addition of 2D NMR spectra for novel compounds. The hybrids of cinnamic acids and pyridine derivatives are particularly active compounds with the lowest MIC50 value of 10.4 mu M for p-chloro cinnamic acid and acetyl pyridine derivatives. An alignment-independent 3D QSAR model identified pharmacophoric hotspots and suggested several structural modifications that might improve the potency of this class of compounds against A. baumannii. The compounds are strong iron-chelating agents forming complexes with a stability constant between 10 7 and 10 9 . The synthesized derivatives represent a promising class of antibacterial compounds with activities comparable to the commonly used antibiotics.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Molecular Structure",
title = "In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens",
volume = "1262",
doi = "10.1016/j.molstruc.2022.133016"
}

Assaleh, M. H., Jeremić, S., Cvijeti, I., Marinkovi, A.,& Prlainovi, N.. (2022). In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens. in Journal of Molecular Structure
Elsevier, Amsterdam., 1262.
https://doi.org/10.1016/j.molstruc.2022.133016

Assaleh MH, Jeremić S, Cvijeti I, Marinkovi A, Prlainovi N. In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens. in Journal of Molecular Structure. 2022;1262.
doi:10.1016/j.molstruc.2022.133016 .

Assaleh, Mohamed H., Jeremić, Sanja, Cvijeti, Ilija, Marinkovi, Aleksandar, Prlainovi, Nevena, "In vitro activity of novel cinnamic acids hydrazides against clinically important pathogens" in Journal of Molecular Structure, 1262 (2022),
https://doi.org/10.1016/j.molstruc.2022.133016 . .

TY  - JOUR
AU  - Ugrin, Milena
AU  - Dinić, Jelena
AU  - Jeremić, Sanja
AU  - Dragičević, Sandra
AU  - Banović Đeri, Bojana
AU  - Nikolić, Aleksandra
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1478
AB  - Bacterial nanocellulose (BNC) stands out among polymers as a promising biomaterial due to its mechanical strength, hydrophilicity, biocompatibility, biodegradability, low toxicity and renewability. The use of scaffolds based on BNC for 3D cell culture has been previously demonstrated. The study exploited excellent properties of the BNC to develop an efficient and low-cost in vitro cell migration assay. The BNC scaffold was introduced into a cell culture 24 h after the SW480 cells were seeded, and cells were allowed to enter the scaffold within the next 24-48 h. The cells were stained with different fluorophores either before or after the introduction of the scaffold in the culture. Untreated cells were observed to enter the BNC scaffold in significant numbers, form clusters and retain a high viability after 48 h. To validate the assay's usability for drug development, the treatments of SW480 cells were performed using aspirin, an agent known to reduce the migratory potential of this cell line in culture. This study demonstrates the application of BNC as a scaffold for cell migration testing as a low-cost alternative to commercial assays based on the Boyden chamber principle. The assay could be further developed for routine use in cancer research and anticancer drug development.
PB  - MDPI, Basel
T2  - Nanomaterials
T1  - Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay
IS  - 9
VL  - 11
DO  - 10.3390/nano11092322
ER  - 

@article{
author = "Ugrin, Milena and Dinić, Jelena and Jeremić, Sanja and Dragičević, Sandra and Banović Đeri, Bojana and Nikolić, Aleksandra",
year = "2021",
abstract = "Bacterial nanocellulose (BNC) stands out among polymers as a promising biomaterial due to its mechanical strength, hydrophilicity, biocompatibility, biodegradability, low toxicity and renewability. The use of scaffolds based on BNC for 3D cell culture has been previously demonstrated. The study exploited excellent properties of the BNC to develop an efficient and low-cost in vitro cell migration assay. The BNC scaffold was introduced into a cell culture 24 h after the SW480 cells were seeded, and cells were allowed to enter the scaffold within the next 24-48 h. The cells were stained with different fluorophores either before or after the introduction of the scaffold in the culture. Untreated cells were observed to enter the BNC scaffold in significant numbers, form clusters and retain a high viability after 48 h. To validate the assay's usability for drug development, the treatments of SW480 cells were performed using aspirin, an agent known to reduce the migratory potential of this cell line in culture. This study demonstrates the application of BNC as a scaffold for cell migration testing as a low-cost alternative to commercial assays based on the Boyden chamber principle. The assay could be further developed for routine use in cancer research and anticancer drug development.",
publisher = "MDPI, Basel",
journal = "Nanomaterials",
title = "Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay",
number = "9",
volume = "11",
doi = "10.3390/nano11092322"
}

Ugrin, M., Dinić, J., Jeremić, S., Dragičević, S., Banović Đeri, B.,& Nikolić, A.. (2021). Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay. in Nanomaterials
MDPI, Basel., 11(9).
https://doi.org/10.3390/nano11092322

Ugrin M, Dinić J, Jeremić S, Dragičević S, Banović Đeri B, Nikolić A. Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay. in Nanomaterials. 2021;11(9).
doi:10.3390/nano11092322 .

Ugrin, Milena, Dinić, Jelena, Jeremić, Sanja, Dragičević, Sandra, Banović Đeri, Bojana, Nikolić, Aleksandra, "Bacterial Nanocellulose as a Scaffold for In Vitro Cell Migration Assay" in Nanomaterials, 11, no. 9 (2021),
https://doi.org/10.3390/nano11092322 . .

TY  - JOUR
AU  - Jeremić, Sanja
AU  - Milovanović, Jelena
AU  - Mojicević, Marija
AU  - Škaro Bogojević, Sanja
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1343
AB  - Plastic pollution is now considered one of the largest environmental threats facing humans and animals globally. Development of bioplastic materials may offer part of the solution as bioplastics include both nondegradable and biodegradable materials with both being important for sustainability. Bioplastic materials are currently being designed to encompass minimal carbon footprint, high recycling value and complete biodegradability. This review examines recent developments and trends in the field of bioplastic materials. A range of the most utilized bioplastic materials is presented (poly(lactic acid) (PLA), polyhydroxyalkanoate (PHA), starch, cellulose, bio-based poly(butylene succinate) (bio-PBS) and bio-polyethylene (bio-PE)) including their production, application and degradation options.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Understanding bioplastic materials - Current state and trends
EP  - 1538
IS  - 12
SP  - 1507
VL  - 85
DO  - 10.2298/JSC200720051J
ER  - 

@article{
author = "Jeremić, Sanja and Milovanović, Jelena and Mojicević, Marija and Škaro Bogojević, Sanja and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Plastic pollution is now considered one of the largest environmental threats facing humans and animals globally. Development of bioplastic materials may offer part of the solution as bioplastics include both nondegradable and biodegradable materials with both being important for sustainability. Bioplastic materials are currently being designed to encompass minimal carbon footprint, high recycling value and complete biodegradability. This review examines recent developments and trends in the field of bioplastic materials. A range of the most utilized bioplastic materials is presented (poly(lactic acid) (PLA), polyhydroxyalkanoate (PHA), starch, cellulose, bio-based poly(butylene succinate) (bio-PBS) and bio-polyethylene (bio-PE)) including their production, application and degradation options.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Understanding bioplastic materials - Current state and trends",
pages = "1538-1507",
number = "12",
volume = "85",
doi = "10.2298/JSC200720051J"
}

Jeremić, S., Milovanović, J., Mojicević, M., Škaro Bogojević, S.,& Nikodinović-Runić, J.. (2020). Understanding bioplastic materials - Current state and trends. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 85(12), 1507-1538.
https://doi.org/10.2298/JSC200720051J

Jeremić S, Milovanović J, Mojicević M, Škaro Bogojević S, Nikodinović-Runić J. Understanding bioplastic materials - Current state and trends. in Journal of the Serbian Chemical Society. 2020;85(12):1507-1538.
doi:10.2298/JSC200720051J .

Jeremić, Sanja, Milovanović, Jelena, Mojicević, Marija, Škaro Bogojević, Sanja, Nikodinović-Runić, Jasmina, "Understanding bioplastic materials - Current state and trends" in Journal of the Serbian Chemical Society, 85, no. 12 (2020):1507-1538,
https://doi.org/10.2298/JSC200720051J . .

TY  - JOUR
AU  - Simić, Stefan
AU  - Jeremić, Sanja
AU  - Đokić, Lidija
AU  - Bozić, Nataša
AU  - Vujcić, Zoran
AU  - Loncar, Nikola
AU  - Senthamaraikannan, Ramsankar
AU  - Babu, Ramesh
AU  - Opsenica, Igor M.
AU  - Nikodinović-Runić, Jasmina
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1406
AB  - Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7-24 h with good yields (70-99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 degrees C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.
PB  - New York : Elsevier Science Inc
T2  - Enzyme and Microbial Technology
T1  - Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines
VL  - 132
DO  - 10.1016/j.enzmictec.2019.109411
ER  - 

@article{
author = "Simić, Stefan and Jeremić, Sanja and Đokić, Lidija and Bozić, Nataša and Vujcić, Zoran and Loncar, Nikola and Senthamaraikannan, Ramsankar and Babu, Ramesh and Opsenica, Igor M. and Nikodinović-Runić, Jasmina",
year = "2020",
abstract = "Biocatalytic oxidations mediated by laccases are gaining importance due to their versatility and beneficial environmental effects. In this study, the oxidation of 1,4-dihydropyridines has been performed using three different types of bacterial laccase-based catalysts: purified laccase from Bacillus licheniformis ATCC 9945a (BliLacc), Escherichia coli whole cells expressing this laccase, and bacterial nanocellulose (BNC) supported BliLacc catalysts. The catalysts based on bacterial laccase were compared to the commercially available Trametes versicolor laccase (TvLacc). The oxidation product of 2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate was obtained within 7-24 h with good yields (70-99%) with all three biocatalysts. The substrate scope was examined with five additional 1,4-dihydropyridines, one of which was oxidized in high yield. Whole-cell biocatalyst was stable when stored for up to 1-month at 4 degrees C. In addition, evidence has been provided that multicopper oxidase CueO from the E. coli expression host contributed to the oxidation efficiency of the whole-cell biocatalyst. The immobilized whole-cell biocatalyst showed satisfactory activity and retained 37% of its original activity after three biotransformation cycles.",
publisher = "New York : Elsevier Science Inc",
journal = "Enzyme and Microbial Technology",
title = "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines",
volume = "132",
doi = "10.1016/j.enzmictec.2019.109411"
}

Simić, S., Jeremić, S., Đokić, L., Bozić, N., Vujcić, Z., Loncar, N., Senthamaraikannan, R., Babu, R., Opsenica, I. M.,& Nikodinović-Runić, J.. (2020). Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology
New York : Elsevier Science Inc., 132.
https://doi.org/10.1016/j.enzmictec.2019.109411

Simić S, Jeremić S, Đokić L, Bozić N, Vujcić Z, Loncar N, Senthamaraikannan R, Babu R, Opsenica IM, Nikodinović-Runić J. Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines. in Enzyme and Microbial Technology. 2020;132.
doi:10.1016/j.enzmictec.2019.109411 .

Simić, Stefan, Jeremić, Sanja, Đokić, Lidija, Bozić, Nataša, Vujcić, Zoran, Loncar, Nikola, Senthamaraikannan, Ramsankar, Babu, Ramesh, Opsenica, Igor M., Nikodinović-Runić, Jasmina, "Development of an efficient biocatalytic system based on bacterial laccase for the oxidation of selected 1,4-dihydropyridines" in Enzyme and Microbial Technology, 132 (2020),
https://doi.org/10.1016/j.enzmictec.2019.109411 . .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Nikolić, Marija S.
AU  - Stevanović, Sanja
AU  - Nikodinović-Runić, Jasmina
AU  - Jeremić, Sanja
AU  - Pavić, Aleksandar
AU  - Đonlagić, Jasna
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1354
AB  - Star-shaped polymers of biodegradable aliphatic polyester, poly(epsilon-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(epsilon-caprolactone)s,sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation ofsPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation revealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model,Danio rerio), suggested those polymeric materials as suitable for biomedical application.
PB  - Sage Publications Ltd, London
T2  - Journal of Bioactive and Compatible Polymers
T1  - Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects
EP  - 537
IS  - 6
SP  - 517
VL  - 35
DO  - 10.1177/0883911520951826
ER  - 

@article{
author = "Ponjavić, Marijana and Nikolić, Marija S. and Stevanović, Sanja and Nikodinović-Runić, Jasmina and Jeremić, Sanja and Pavić, Aleksandar and Đonlagić, Jasna",
year = "2020",
abstract = "Star-shaped polymers of biodegradable aliphatic polyester, poly(epsilon-caprolactone), PCL, with different number of arms (three, four, and six) were synthesized by ring-opening polymerization initiated by multifunctional alcohols used as cores. As potential biomaterials, synthesized star-shaped poly(epsilon-caprolactone)s,sPCL, were thoroughly characterized in terms of their degradation under different pH conditions and in respect to their cytotoxicity. The in vitro degradation was performed in phosphate buffer (pH 7.4) and hydrochloric acid solution (pH 1.0) over 5 weeks. Degradation ofsPCL films was followed by the weight loss measurements, GPC, FTIR, and AFM analysis. While the most of the samples were stable against the abiotic hydrolysis at pH 7.4 after 5 weeks of degradation, degradation was significantly accelerated in the acidic medium. Degradation rate of polymer films was affected by the polymer architecture and molecular weight. The molecular weight profiles during the degradation revealed random chain scission of the ester bonds indicating bulk degradation mechanism of hydrolysis at pH 7.4, while acidic hydrolysis proceeded through the bulk degradation associated with surface erosion, confirmed by AFM. The in vitro toxicity tests, cytotoxicity applying normal human fibroblasts (MRC5) and embryotoxicity assessment (using zebra fish model,Danio rerio), suggested those polymeric materials as suitable for biomedical application.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Bioactive and Compatible Polymers",
title = "Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects",
pages = "537-517",
number = "6",
volume = "35",
doi = "10.1177/0883911520951826"
}

Ponjavić, M., Nikolić, M. S., Stevanović, S., Nikodinović-Runić, J., Jeremić, S., Pavić, A.,& Đonlagić, J.. (2020). Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects. in Journal of Bioactive and Compatible Polymers
Sage Publications Ltd, London., 35(6), 517-537.
https://doi.org/10.1177/0883911520951826

Ponjavić M, Nikolić MS, Stevanović S, Nikodinović-Runić J, Jeremić S, Pavić A, Đonlagić J. Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects. in Journal of Bioactive and Compatible Polymers. 2020;35(6):517-537.
doi:10.1177/0883911520951826 .

Ponjavić, Marijana, Nikolić, Marija S., Stevanović, Sanja, Nikodinović-Runić, Jasmina, Jeremić, Sanja, Pavić, Aleksandar, Đonlagić, Jasna, "Hydrolytic degradation of star-shaped poly(epsilon-caprolactone)s with different number of arms and their cytotoxic effects" in Journal of Bioactive and Compatible Polymers, 35, no. 6 (2020):517-537,
https://doi.org/10.1177/0883911520951826 . .

TY  - JOUR
AU  - Rajković, Katarina M.
AU  - Vasić, Marijana
AU  - Drobac, Milica
AU  - Mutić, Jelena
AU  - Jeremić, Sanja
AU  - Simić, Valentina
AU  - Stanković, Jovan
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1378
AB  - The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concentrations (0-96% (v/v)) and solvent-to-solid ratios (5-20 kg kg(-1)). The response surface methodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) were developed to optimize the extraction variables. The RSM and ANN-GA models determined 50% (v/v) ethanol concentration and 20 kg kg(-1) solvent-to-solid ratio as optimal conditions, ensuring an extraction yield of 27.69 and 27.19 g 100 g(-1) of dry leaves. The phenolic compounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g(-1)of dry leaves), quercetin-3-O-galactoside (10.99 mg g(-1) of dry leaves) and quercetin 3 0 rhamnoside (15.07 mg g(-1)of dry leaves) using high-performance liquid chromatography (HPLC). The minerals in optimal extract were quantified: macro-elements (the relative order by content was: K  gt  Mg  gt  Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) and micro-elements (the relative order by content was: Zn  gt  Rb  gt  Mn  gt  I gt Sr  gt  Ni  gt  Cu  gt  Co  gt  V  gt  Ag  gt  Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extraction coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%). Optimization of extraction process resulted in high extraction yield from J. nigra leaves and optimal extract containing different phytochemical compounds.
PB  - Elsevier, Amsterdam
T2  - Chemical Engineering Research & Design
T1  - Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves
EP  - 33
SP  - 25
VL  - 157
DO  - 10.1016/j.cherd.2020.03.002
ER  - 

@article{
author = "Rajković, Katarina M. and Vasić, Marijana and Drobac, Milica and Mutić, Jelena and Jeremić, Sanja and Simić, Valentina and Stanković, Jovan",
year = "2020",
abstract = "The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concentrations (0-96% (v/v)) and solvent-to-solid ratios (5-20 kg kg(-1)). The response surface methodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) were developed to optimize the extraction variables. The RSM and ANN-GA models determined 50% (v/v) ethanol concentration and 20 kg kg(-1) solvent-to-solid ratio as optimal conditions, ensuring an extraction yield of 27.69 and 27.19 g 100 g(-1) of dry leaves. The phenolic compounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g(-1)of dry leaves), quercetin-3-O-galactoside (10.99 mg g(-1) of dry leaves) and quercetin 3 0 rhamnoside (15.07 mg g(-1)of dry leaves) using high-performance liquid chromatography (HPLC). The minerals in optimal extract were quantified: macro-elements (the relative order by content was: K  gt  Mg  gt  Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) and micro-elements (the relative order by content was: Zn  gt  Rb  gt  Mn  gt  I gt Sr  gt  Ni  gt  Cu  gt  Co  gt  V  gt  Ag  gt  Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extraction coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%). Optimization of extraction process resulted in high extraction yield from J. nigra leaves and optimal extract containing different phytochemical compounds.",
publisher = "Elsevier, Amsterdam",
journal = "Chemical Engineering Research & Design",
title = "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves",
pages = "33-25",
volume = "157",
doi = "10.1016/j.cherd.2020.03.002"
}

Rajković, K. M., Vasić, M., Drobac, M., Mutić, J., Jeremić, S., Simić, V.,& Stanković, J.. (2020). Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research & Design
Elsevier, Amsterdam., 157, 25-33.
https://doi.org/10.1016/j.cherd.2020.03.002

Rajković KM, Vasić M, Drobac M, Mutić J, Jeremić S, Simić V, Stanković J. Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research & Design. 2020;157:25-33.
doi:10.1016/j.cherd.2020.03.002 .

Rajković, Katarina M., Vasić, Marijana, Drobac, Milica, Mutić, Jelena, Jeremić, Sanja, Simić, Valentina, Stanković, Jovan, "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves" in Chemical Engineering Research & Design, 157 (2020):25-33,
https://doi.org/10.1016/j.cherd.2020.03.002 . .

TY  - JOUR
AU  - Mandić, Mina
AU  - Đokić, Lidija
AU  - Nikolaivits, Efstratios
AU  - Prodanović, Radivoje
AU  - O'Connor, Kevin
AU  - Jeremić, Sanja
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1284
AB  - Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes.
PB  - MDPI, Basel
T2  - Catalysts
T1  - Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes
IS  - 7
VL  - 9
DO  - 10.3390/catal9070629
ER  - 

@article{
author = "Mandić, Mina and Đokić, Lidija and Nikolaivits, Efstratios and Prodanović, Radivoje and O'Connor, Kevin and Jeremić, Sanja and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2019",
abstract = "Laccases are multicopper-oxidases with variety of biotechnological applications. While predominantly used, fungal laccases have limitations such as narrow pH and temperature range and their production via heterologous protein expression is more complex due to posttranslational modifications. In comparison, bacterial enzymes, including laccases, usually possess higher thermal and pH stability, and are more suitable for expression and genetic manipulations in bacterial expression hosts. Therefore, the aim of this study was to identify, recombinantly express, and characterize novel laccases from Pseudomonas spp. A combination of approaches including DNA sequence analysis, N-terminal protein sequencing, and genome sequencing data analysis for laccase amplification, cloning, and overexpression have been used. Four active recombinant laccases were obtained, one each from P. putida KT2440 and P. putida CA-3, and two from P. putida F6. The new laccases exhibited broad temperature and pH range and high thermal stability, as well as the potential to degrade selection of synthetic textile dyes. The best performing laccase was CopA from P. putida F6 which degraded five out of seven tested dyes, including Amido Black 10B, Brom Cresol Purple, Evans Blue, Reactive Black 5, and Remazol Brilliant Blue. This work highlighted species of Pseudomonas genus as still being good sources of biocatalytically relevant enzymes.",
publisher = "MDPI, Basel",
journal = "Catalysts",
title = "Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes",
number = "7",
volume = "9",
doi = "10.3390/catal9070629"
}

Mandić, M., Đokić, L., Nikolaivits, E., Prodanović, R., O'Connor, K., Jeremić, S., Topakas, E.,& Nikodinović-Runić, J.. (2019). Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes. in Catalysts
MDPI, Basel., 9(7).
https://doi.org/10.3390/catal9070629

Mandić M, Đokić L, Nikolaivits E, Prodanović R, O'Connor K, Jeremić S, Topakas E, Nikodinović-Runić J. Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes. in Catalysts. 2019;9(7).
doi:10.3390/catal9070629 .

Mandić, Mina, Đokić, Lidija, Nikolaivits, Efstratios, Prodanović, Radivoje, O'Connor, Kevin, Jeremić, Sanja, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Identification and Characterization of New Laccase Biocatalysts from Pseudomonas Species Suitable for Degradation of Synthetic Textile Dyes" in Catalysts, 9, no. 7 (2019),
https://doi.org/10.3390/catal9070629 . .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Veselinović, Ljiljana
AU  - Ražić, Slavica
AU  - Jeremić, Sanja
AU  - Filipič, Metka
AU  - Žegura, Bojana
AU  - Tomić, Sergej
AU  - Čolić, Miodrag
AU  - Stevanović, Magdalena
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1613
AB  - Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.
PB  - Elsevier
T2  - Materials Science and Engineering C
T1  - Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles
EP  - 789
SP  - 776
VL  - 96
DO  - 10.1016/j.msec.2018.11.073
ER  - 

@article{
author = "Filipović, Nenad and Veselinović, Ljiljana and Ražić, Slavica and Jeremić, Sanja and Filipič, Metka and Žegura, Bojana and Tomić, Sergej and Čolić, Miodrag and Stevanović, Magdalena",
year = "2019",
abstract = "Poly (ε-caprolactone) (PCL) microspheres as a carrier for sustained release of antibacterial agent, selenium nanoparticles (SeNPs), were developed. The obtained PCL/SeNPs microspheres were in the range 1–4 μm with the encapsulation efficiency of about 90%. The degradation process and release behavior of SeNPs from PCL microspheres were investigated in five different degradation media: phosphate buffer solution (PBS), a solution of lipase isolated from the porcine pancreas in PBS, 0.1 M hydrochloric acid (HCl), Pseudomonas aeruginosa PAO1 cell-free extract in PBS and implant fluid (exudate) from the subcutaneously implanted sterile polyvinyl sponges which induce a foreign-body inflammatory reaction. The samples were thoroughly characterized by SEM, TEM, FTIR, XRD, PSA, DSC, confocal microscopy, and ICP-OES techniques. Under physiological conditions at neutral pH, a very slow release of SeNPs occurred (3 and 8% in the case of PBS or PBS + lipase, respectively and after 660 days), while in the acidic environment their presence was not detected. On the other hand, the release in the medium with bacterial extract was much more pronounced, even after 24 h (13%). After 7 days, the concentration of SeNPs reached a maximum of around 30%. Also, 37% of SeNPs have been released after 11 days of incubation of PCL/SeNPs in the implant exudate. These results suggest that the release of SeNPs from PCL was triggered by Pseudomonas aeruginosa PAO1 bacterium as well as by foreign body inflammatory reaction to implant. Furthermore, PCL/SeNPs microspheres were investigated in terms of their biocompatibility. For this purpose, cytotoxicity, the formation of reactive oxygen species (ROS), and genotoxicity were evaluated on HepG2 cell line. The interaction of PCL/SeNPs with phagocytic cell line (Raw 264.7 macrophages) was monitored as well. It was found that the microspheres in investigated concentration range had no acute cytotoxic effects. Finally, SeNPs, as well as PCL/SeNPs, showed a considerable antibacterial activity against Gram-positive bacteria: Staphylococcus aureus (ATCC 25923) and Staphylococcus epidermidis (ATCC 1228). These results suggest that PCL/SeNPs-based system could be an attractive platform for a prolonged prevention of infections accompanying implants. © 2018 Elsevier B.V.",
publisher = "Elsevier",
journal = "Materials Science and Engineering C",
title = "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles",
pages = "789-776",
volume = "96",
doi = "10.1016/j.msec.2018.11.073"
}

Filipović, N., Veselinović, L., Ražić, S., Jeremić, S., Filipič, M., Žegura, B., Tomić, S., Čolić, M.,& Stevanović, M.. (2019). Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C
Elsevier., 96, 776-789.
https://doi.org/10.1016/j.msec.2018.11.073

Filipović N, Veselinović L, Ražić S, Jeremić S, Filipič M, Žegura B, Tomić S, Čolić M, Stevanović M. Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles. in Materials Science and Engineering C. 2019;96:776-789.
doi:10.1016/j.msec.2018.11.073 .

Filipović, Nenad, Veselinović, Ljiljana, Ražić, Slavica, Jeremić, Sanja, Filipič, Metka, Žegura, Bojana, Tomić, Sergej, Čolić, Miodrag, Stevanović, Magdalena, "Poly (ε-caprolactone) microspheres for prolonged release of selenium nanoparticles" in Materials Science and Engineering C, 96 (2019):776-789,
https://doi.org/10.1016/j.msec.2018.11.073 . .

TY  - JOUR
AU  - Mandić, Mina
AU  - Spasić, Jelena
AU  - Ponjavić, Marijana
AU  - Nikolić, Marija S.
AU  - Cosović, Vladan R.
AU  - O'Connor, Kevin
AU  - Nikodinović-Runić, Jasmina
AU  - Đokić, Lidija
AU  - Jeremić, Sanja
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1760
AB  - Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(epsilon-caprolactone) (PCL) or poly-hydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 mu m) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 mu m) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chiororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis13-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.
PB  - Elsevier Sci Ltd, Oxford
T2  - Polymer Degradation and Stability
T1  - Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil
EP  - 168
SP  - 160
VL  - 162
DO  - 10.1016/j.polymdegradstab.2019.02.012
ER  - 

@article{
author = "Mandić, Mina and Spasić, Jelena and Ponjavić, Marijana and Nikolić, Marija S. and Cosović, Vladan R. and O'Connor, Kevin and Nikodinović-Runić, Jasmina and Đokić, Lidija and Jeremić, Sanja",
year = "2019",
abstract = "Petrochemical plastics are generally recalcitrant to microbial degradation and accumulate in the environment. Biodegradable polymers obtained synthetically like poly(epsilon-caprolactone) (PCL) or poly-hydroxyalkanoates (PHA), obtained biotechnologically, have shown great potential as a replacement for petroleum-based plastics. Nevertheless, their biodegradation and environmental faith have been less examined. In this study, thin films of PCL (200 mu m) and medium chain length PHA (mcl-PHA, 70 M fraction of 3-hydroxyoctanoate and 30 M fraction of 3-hydroxydecanoate, 600 mu m) were exposed to total protein preparations (extracellular proteins combined with a crude cell extract) of soil isolates Pseudomonas chiororaphis B-561 and Streptomyces sp. BV315 that had been grown on waste cooking oil as a sole carbon source. Biodegradation potential of two polyesters was evaluated in buffer with total protein preparations and in a laboratory compost model system augmented with selected bacteria. Overall, PCL showed better biodegradation properties in comparison to mcl-PHA. Both materials showed surface erosion after 4-weeks of exposure to total protein preparations of both strains, with a moderate weight loss of 1.3% when P. chlororaphis13-561 was utilized. In laboratory compost model system PCL and mcl-PHA showed significant weight loss ranging from 13 to 17% when Streptomyces sp. BV315 culture was used. Similar weight loss of PCL and mcl-PHA was achieved for 4 and 8 weeks, respectively indicating slower degradation of mcl-PHA. Growth on waste cooking oil as a sole carbon source increased the potential of both tested strains to degrade PCL and mcl-PHA, making them good candidates for augmentation of compost cultures in waste management of both waste cooking oils and biodegradable polymers.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Polymer Degradation and Stability",
title = "Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil",
pages = "168-160",
volume = "162",
doi = "10.1016/j.polymdegradstab.2019.02.012"
}

Mandić, M., Spasić, J., Ponjavić, M., Nikolić, M. S., Cosović, V. R., O'Connor, K., Nikodinović-Runić, J., Đokić, L.,& Jeremić, S.. (2019). Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability
Elsevier Sci Ltd, Oxford., 162, 160-168.
https://doi.org/10.1016/j.polymdegradstab.2019.02.012

Mandić M, Spasić J, Ponjavić M, Nikolić MS, Cosović VR, O'Connor K, Nikodinović-Runić J, Đokić L, Jeremić S. Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil. in Polymer Degradation and Stability. 2019;162:160-168.
doi:10.1016/j.polymdegradstab.2019.02.012 .

Mandić, Mina, Spasić, Jelena, Ponjavić, Marijana, Nikolić, Marija S., Cosović, Vladan R., O'Connor, Kevin, Nikodinović-Runić, Jasmina, Đokić, Lidija, Jeremić, Sanja, "Biodegradation of poly(epsilon-caprolactone) (PCL) and medium chain length polyhydroxyalkanoate (mcl-PHA) using whole cells and cell free protein preparations of Pseudomonas and Streptomyces strains grown on waste cooking oil" in Polymer Degradation and Stability, 162 (2019):160-168,
https://doi.org/10.1016/j.polymdegradstab.2019.02.012 . .