TY  - CONF
AU  - Kojić, Snežana
AU  - Bošković, Srđan
AU  - Milovanović, Mina
AU  - Stainie, Didier
AU  - Juez, Rubén Marín
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
PY  - 2024
UR  - https://www.izfs.org/education/10sczi
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2309
AB  - In contrast to humans, zebrafish have a remarkable ability to regenerate their hearts after injury, while both humans and zebrafish efficiently repair the wounded skeletal muscle. Common players in these two processes might represent potential targets for the development of efficient therapies to stimulate human heart to regenerate after injury. We identified ankrd1a expression to be upregulated in both regenerating zebrafish hearts and in repairing skeletal muscle. Its mammalian homolog ANKRD1/CARP encodes a stress responsive cardiac ankyrin repeat protein involved in transcriptional regulation, sarcomere assembly and mechanosensing. Using a TgBAC(ankrd1a:EGFP) line, we showed that activation of ankrd1a in cryoinjured heart is restricted to border zone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of regenerating cardiomyocytes. After stab wound injury of skeletal muscle expression of the fluorescent reporter was observed from 3 dpi, when new EGFP-positive muscle cells emerged inside the injury zone. At later time points, EGFP-positive myofibers were visible in the deeper tissue layers, concomitant with active repair of the injured tissue. In cryoinjured skeletal muscle, strong activation of ankrd1a was also observed in myofibers adjacent to the injury, and in those on uninjured side. Detection of the transgene in both newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury suggests the role of ankrd1a in skeletal muscle tissue repair and adaptive processes in uninjured myofibers surrounding the injury site. Our results implicate ankrd1a in zebrafish muscle regeneration, repair and remodeling, promoting it as an attractive target for translational studies, as a player in muscle healing and as a sensor of stressed muscle.
PB  - Society for Zebrafish Research
C3  - 10th Strategic Conference of Zebrafish Investigators
T1  - Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2309
ER  - 

@conference{
author = "Kojić, Snežana and Bošković, Srđan and Milovanović, Mina and Stainie, Didier and Juez, Rubén Marín and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija",
year = "2024",
abstract = "In contrast to humans, zebrafish have a remarkable ability to regenerate their hearts after injury, while both humans and zebrafish efficiently repair the wounded skeletal muscle. Common players in these two processes might represent potential targets for the development of efficient therapies to stimulate human heart to regenerate after injury. We identified ankrd1a expression to be upregulated in both regenerating zebrafish hearts and in repairing skeletal muscle. Its mammalian homolog ANKRD1/CARP encodes a stress responsive cardiac ankyrin repeat protein involved in transcriptional regulation, sarcomere assembly and mechanosensing. Using a TgBAC(ankrd1a:EGFP) line, we showed that activation of ankrd1a in cryoinjured heart is restricted to border zone cardiomyocytes, implicating this gene in dedifferentiation and proliferation of regenerating cardiomyocytes. After stab wound injury of skeletal muscle expression of the fluorescent reporter was observed from 3 dpi, when new EGFP-positive muscle cells emerged inside the injury zone. At later time points, EGFP-positive myofibers were visible in the deeper tissue layers, concomitant with active repair of the injured tissue. In cryoinjured skeletal muscle, strong activation of ankrd1a was also observed in myofibers adjacent to the injury, and in those on uninjured side. Detection of the transgene in both newly formed myofibers that invade the wound and in the apparently uninjured tissue surrounding the injury suggests the role of ankrd1a in skeletal muscle tissue repair and adaptive processes in uninjured myofibers surrounding the injury site. Our results implicate ankrd1a in zebrafish muscle regeneration, repair and remodeling, promoting it as an attractive target for translational studies, as a player in muscle healing and as a sensor of stressed muscle.",
publisher = "Society for Zebrafish Research",
journal = "10th Strategic Conference of Zebrafish Investigators",
title = "Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2309"
}

Kojić, S., Bošković, S., Milovanović, M., Stainie, D., Juez, R. M., Jasnić, J., Novković, M.,& Milošević, E.. (2024). Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair. in 10th Strategic Conference of Zebrafish Investigators
Society for Zebrafish Research..
https://hdl.handle.net/21.15107/rcub_imagine_2309

Kojić S, Bošković S, Milovanović M, Stainie D, Juez RM, Jasnić J, Novković M, Milošević E. Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair. in 10th Strategic Conference of Zebrafish Investigators. 2024;.
https://hdl.handle.net/21.15107/rcub_imagine_2309 .

Kojić, Snežana, Bošković, Srđan, Milovanović, Mina, Stainie, Didier, Juez, Rubén Marín, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, "Zebrafish ankrd1a as a common player  in heart regeneration and skeletal muscle repair" in 10th Strategic Conference of Zebrafish Investigators (2024),
https://hdl.handle.net/21.15107/rcub_imagine_2309 .

TY  - JOUR
AU  - Milošević, Emilija
AU  - Novković, Mirjana
AU  - Cenni, Vittoria
AU  - Bavelloni, Alberto
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2024
UR  - https://doi.org/10.1007/s00418-024-02272-2
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2325
AB  - Rhabdomyosarcoma (RMS) is the most common soft tissue malignancy in children and adolescents. Respecting the age of the patients and the tumor aggressiveness, investigation of the molecular mechanisms of RMS tumorigenesis is directed toward the identification of novel therapeutic targets. To contribute to a better understanding of the molecular pathology of RMS, we investigated ankyrin repeat domain 1 (ANKRD1), designated as a potential marker for differential diagnostics. In this study, we used three RMS cell lines (SJRH30, RD, and HS-729) to assess its expression profile, intracellular localization, and turnover. They express wild-type ANKRD1, as judged by the sequencing of the open reading frame. Each cell line expressed a different amount of ANKRD1 protein, although the transcript level was similar. According to western blot analysis, ANKRD1 protein was expressed at detectable levels in the SJRH30 and RD cells (SJRH30 > RD), but not in the HS-729, even after immunoprecipitation. Immunocytochemistry revealed nuclear and cytoplasmic localization of ANKRD1 in all examined cell lines. Moreover, the punctate pattern of ANKRD1 staining in the nuclei of RD and HS-729 cells overlapped with coilin, indicating its association with Cajal bodies. We have shown that RMS cells are not able to overexpress ANKRD1 protein, which can be attributed to its proteasomal degradation. The unsuccessful attempt to overexpress ANKRD1 in RMS cells indicates the possibility that its overexpression may have detrimental effects for RMS cells and opens a window for further research into its role in RMS pathogenesis and for potential therapeutic targeting.
PB  - Springer Nature
T2  - Histochemistry and Cell Biology
T2  - Histochemistry and Cell BiologyHistochem Cell Biol
T1  - Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation
DO  - 10.1007/s00418-024-02272-2
ER  - 

@article{
author = "Milošević, Emilija and Novković, Mirjana and Cenni, Vittoria and Bavelloni, Alberto and Kojić, Snežana and Jasnić, Jovana",
year = "2024",
abstract = "Rhabdomyosarcoma (RMS) is the most common soft tissue malignancy in children and adolescents. Respecting the age of the patients and the tumor aggressiveness, investigation of the molecular mechanisms of RMS tumorigenesis is directed toward the identification of novel therapeutic targets. To contribute to a better understanding of the molecular pathology of RMS, we investigated ankyrin repeat domain 1 (ANKRD1), designated as a potential marker for differential diagnostics. In this study, we used three RMS cell lines (SJRH30, RD, and HS-729) to assess its expression profile, intracellular localization, and turnover. They express wild-type ANKRD1, as judged by the sequencing of the open reading frame. Each cell line expressed a different amount of ANKRD1 protein, although the transcript level was similar. According to western blot analysis, ANKRD1 protein was expressed at detectable levels in the SJRH30 and RD cells (SJRH30 > RD), but not in the HS-729, even after immunoprecipitation. Immunocytochemistry revealed nuclear and cytoplasmic localization of ANKRD1 in all examined cell lines. Moreover, the punctate pattern of ANKRD1 staining in the nuclei of RD and HS-729 cells overlapped with coilin, indicating its association with Cajal bodies. We have shown that RMS cells are not able to overexpress ANKRD1 protein, which can be attributed to its proteasomal degradation. The unsuccessful attempt to overexpress ANKRD1 in RMS cells indicates the possibility that its overexpression may have detrimental effects for RMS cells and opens a window for further research into its role in RMS pathogenesis and for potential therapeutic targeting.",
publisher = "Springer Nature",
journal = "Histochemistry and Cell Biology, Histochemistry and Cell BiologyHistochem Cell Biol",
title = "Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation",
doi = "10.1007/s00418-024-02272-2"
}

Milošević, E., Novković, M., Cenni, V., Bavelloni, A., Kojić, S.,& Jasnić, J.. (2024). Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation. in Histochemistry and Cell Biology
Springer Nature..
https://doi.org/10.1007/s00418-024-02272-2

Milošević E, Novković M, Cenni V, Bavelloni A, Kojić S, Jasnić J. Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation. in Histochemistry and Cell Biology. 2024;.
doi:10.1007/s00418-024-02272-2 .

Milošević, Emilija, Novković, Mirjana, Cenni, Vittoria, Bavelloni, Alberto, Kojić, Snežana, Jasnić, Jovana, "Molecular characterization of ANKRD1 in rhabdomyosarcoma cell lines: expression, localization, and proteasomal degradation" in Histochemistry and Cell Biology (2024),
https://doi.org/10.1007/s00418-024-02272-2 . .

TY  - CONF
AU  - Milovanović, Mina
AU  - Bošković, Srđan
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Kojić, Snežana
PY  - 2023
UR  - https://zebrafish2023.org/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2023
AB  - In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation
of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close
proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle
regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and
remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal
expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections
prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury
(dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi.
At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry,
while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the
deeper tissue layers within the injury, indicating active repair of the injured tissue. To identify cells
in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells,
undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was
detected both in the newly formed myofibers that invade the wound and in the apparently uninjured
tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged
myofibers surrounding the physical injury.
C3  - 12th European Zebrafish Meeting
T1  - Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle
EP  - 276
SP  - 276
SP  - 0254
VL  - 14
VL  - 12
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2023
ER  - 

@conference{
author = "Milovanović, Mina and Bošković, Srđan and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Kojić, Snežana",
year = "2023",
abstract = "In our previous work, using transgenic zebrafish line TgBAC(ankrd1a:EGFP), we showed activation
of the zebrafish ankrd1a gene in border zone cardiomyocytes of cryoinjured heart and in close
proximity of needle-stab wounds in skeletal muscle, indicating its involvement in muscle
regeneration. Our results implicated ankrd1a in zebrafish skeletal muscle tissue repair and
remodeling, as a sensor of stressed muscle. Here we take a closer look at the spatio-temporal
expression profile of the ankrd1a gene in injured zebrafish skeletal muscle by analyzing cryosections
prepared from wounded tissue of TgBAC(ankrd1a:EGFP) adults at 1, 3, 5, 7 and 10 days post-injury
(dpi). The expression of the fluorescent reporter was observed from 3 dpi and remained until 10 dpi.
At 3dpi, new GFP-positive muscle cells emerged inside the injury zone, at the site of needle entry,
while in the later days (5, 7 and 10 dpi), newly formed GFP-positive myofibers were visible in the
deeper tissue layers within the injury, indicating active repair of the injured tissue. To identify cells
in which ankrd1a is activated after injury, we stained the sections for markers of satellite-like cells,
undifferentiated and differentiated muscle cells, and mature myofibers. Since the reporter was
detected both in the newly formed myofibers that invade the wound and in the apparently uninjured
tissue surrounding the injury, we hypothesize that ankrd1a is not only involved in satellite celldependent tissue repair, but its expression might be a hallmark of adaptive process in undamaged
myofibers surrounding the physical injury.",
journal = "12th European Zebrafish Meeting",
title = "Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle",
pages = "276-276-0254",
volume = "14, 12",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2023"
}

Milovanović, M., Bošković, S., Jasnić, J., Novković, M., Milošević, E.,& Kojić, S.. (2023). Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle. in 12th European Zebrafish Meeting, 14, 276-276.
https://hdl.handle.net/21.15107/rcub_imagine_2023

Milovanović M, Bošković S, Jasnić J, Novković M, Milošević E, Kojić S. Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle. in 12th European Zebrafish Meeting. 2023;14:276-276.
https://hdl.handle.net/21.15107/rcub_imagine_2023 .

Milovanović, Mina, Bošković, Srđan, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Kojić, Snežana, "Expression profile of ankrd1a during repair of injured zebrafish skeletal muscle" in 12th European Zebrafish Meeting, 14 (2023):276-276,
https://hdl.handle.net/21.15107/rcub_imagine_2023 .

TY  - JOUR
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1928
AB  - Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.
PB  - Springer Nature
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines
DO  - 10.1007/s00432-023-04930-9
ER  - 

@article{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
abstract = "Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.",
publisher = "Springer Nature",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines",
doi = "10.1007/s00432-023-04930-9"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology
Springer Nature..
https://doi.org/10.1007/s00432-023-04930-9

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. 2023;.
doi:10.1007/s00432-023-04930-9 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines" in Journal of Cancer Research and Clinical Oncology (2023),
https://doi.org/10.1007/s00432-023-04930-9 . .

TY  - CONF
AU  - Milovanović, Mina
AU  - Bošković, Srđan
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Kojić, Snežana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2132
AB  - Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart
through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of
new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary
vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration.
Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing
ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and
7 days. Dedifferentiating cardiomyocytes and epicardial cells were immunostained with anti-MYH7 and
anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy.
Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between
the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7.
The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating
zebrafish heart.
Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone
cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute
to the regeneration process occuring in these layers of the heart.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Spatial profile of ankrd1a activation during regeneration of zebrafish heart
EP  - 141
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2132
ER  - 

@conference{
author = "Milovanović, Mina and Bošković, Srđan and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Kojić, Snežana",
year = "2023",
abstract = "Introduction: In contrast to humans, zebrafish have a remarkable ability to regenerate injured heart
through a complex and highly orchestrated processinvolving all cardiac structures. The majorsource of
new myocardial cells are resident cardiomyocytes, which dedifferentiate and reinitiate proliferation, invading the area of injury to replace the lost myocardium. The response of the myocardium and coronary
vasculature is preceded by activation of epi- and endocardium, which form active scaffolds to guide regeneration. The aim of thisstudy wasto identify cardiac structuresin which ankrd1a gene is activated during zebrafish heart regeneration.
Methods: We crossed several zebrafish reporter lines: TgBAC(ankrd1a:EGFP) (to identify cells expressing
ankrd1a), Tg(myl7:nls-dsRedExpress) (for labeling cardiomyocyte nuclei) and Tg(kdrl:RAS-mCherry) (for labeling endocardial/endothelial cells). Zebrafish hearts were cryoinjured and left to regenerate for 3 and
7 days. Dedifferentiating cardiomyocytes and epicardial cells were immunostained with anti-MYH7 and
anti-caveolin1 antibody, respectively. Cells labeled with transgenes and immunostaining were visualized on tissue cryosections by fluorescent microscopy.
Results: Zebrafish ankrd1a was activated in the injury border zone cardiomyocytes, located between
the injured and remote myocardium. Its expression preceded that of a dedifferentiation marker, MYH7.
The TgBAC(ankrd1a:EGFP) transgene was not detected in epicardial or endocardial cells of regenerating
zebrafish heart.
Conclusion: Activation of ankrd1a during regeneration of zebrafish heart is restricted to borderzone
cardiomyocytes, implicating this gene in dedifferentiation and proliferation of cardiomyocytes. The absence of ankrd1a expression in epicardium and endocardium indicatesthat this gene does not contribute
to the regeneration process occuring in these layers of the heart.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Spatial profile of ankrd1a activation during regeneration of zebrafish heart",
pages = "141-141",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2132"
}

Milovanović, M., Bošković, S., Jasnić, J., Novković, M., Milošević, E.,& Kojić, S.. (2023). Spatial profile of ankrd1a activation during regeneration of zebrafish heart. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 141-141.
https://hdl.handle.net/21.15107/rcub_imagine_2132

Milovanović M, Bošković S, Jasnić J, Novković M, Milošević E, Kojić S. Spatial profile of ankrd1a activation during regeneration of zebrafish heart. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:141-141.
https://hdl.handle.net/21.15107/rcub_imagine_2132 .

Milovanović, Mina, Bošković, Srđan, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Kojić, Snežana, "Spatial profile of ankrd1a activation during regeneration of zebrafish heart" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):141-141,
https://hdl.handle.net/21.15107/rcub_imagine_2132 .

TY  - JOUR
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1918
AB  - Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.
PB  - Springer Nature
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines
DO  - 10.1007/s00432-023-04930-9
ER  - 

@article{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
abstract = "Sarcomas are rare and heterogenic tumors with unclear etiology. They develop in bone and connective tissue, mainly in pediatric patients. To increase efficacy of current therapeutic options, natural products showing selective toxicity to tumor cells are extensively investigated. Here, we evaluated antitumor activity of bacterial pigment violacein in osteosarcoma (OS) and rhabdomyosarcoma (RMS) cell lines.",
publisher = "Springer Nature",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines",
doi = "10.1007/s00432-023-04930-9"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology
Springer Nature..
https://doi.org/10.1007/s00432-023-04930-9

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. 2023;.
doi:10.1007/s00432-023-04930-9 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines" in Journal of Cancer Research and Clinical Oncology (2023),
https://doi.org/10.1007/s00432-023-04930-9 . .

TY  - CONF
AU  - Novković, Mirjana
AU  - Vasić, Marko
AU  - Jasnić, Jovana
AU  - Milošević, Emilija
AU  - Milovanović, Mina
AU  - Savić, Slobodan
AU  - Kojić, Snežana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2114
AB  - Introduction: Ankyrin Repeat Domain 2 (ANKRD2) is expressed in skeletal muscle, where plays a role in
muscle development, differentiation and adaptation to stress. Human skeletal muscle consists of three
major fiber types: type 1 (slow-twitch, oxidative), type 2A (fast-twitch, oxidative) and type 2X (fast-twitch,
glycolytic). ANKRD2 is reported to be primarily expressed in type 1 myofibers. However, recent findings
on human single myofibers and our study of chicken muscles have shown that this protein may also be
expressed in type 2A fibers. Hence, our objective was to examine whether ANKRD2 is present in human
fast, type 2A muscle fibers using immunohistochemistry.
Methods: Samples of large leg musclessoleus, gastrocnemius, vastusintermedius and vastuslateralis were
obtained from human cadaveric tissue. Serial cryosections were independently stained with anti-ANKRD2
and antibodies for different myosin heavy chain isoforms (6H1 for type 2X, BF35 for type 1 and 2A, antiMHCs for type 1 and anti-MHCf for type 2A and 2X fibers). Immunostained tissues were analyzed by fluorescent microscopy.
Results: In addition to slow, type 1, ANKRD2 wasfound expressed in fast, type 2A myofibers, which both
have oxidative metabolism. Further, we did not observe ANDRD2 expression in glycolytic, type 2X
myiofibers. This pattern of ANKRD2 expression was consistent across all examined muscles.
Conclusion: Our resultsimplicate that the regulatory mechanism of ANKRD2 expression in human skeletal muscle is associated with oxidative metabolism, rather than muscle contraction speed.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Determination of muscle fiber types expressing ANKRD2
EP  - 155
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2114
ER  - 

@conference{
author = "Novković, Mirjana and Vasić, Marko and Jasnić, Jovana and Milošević, Emilija and Milovanović, Mina and Savić, Slobodan and Kojić, Snežana",
year = "2023",
abstract = "Introduction: Ankyrin Repeat Domain 2 (ANKRD2) is expressed in skeletal muscle, where plays a role in
muscle development, differentiation and adaptation to stress. Human skeletal muscle consists of three
major fiber types: type 1 (slow-twitch, oxidative), type 2A (fast-twitch, oxidative) and type 2X (fast-twitch,
glycolytic). ANKRD2 is reported to be primarily expressed in type 1 myofibers. However, recent findings
on human single myofibers and our study of chicken muscles have shown that this protein may also be
expressed in type 2A fibers. Hence, our objective was to examine whether ANKRD2 is present in human
fast, type 2A muscle fibers using immunohistochemistry.
Methods: Samples of large leg musclessoleus, gastrocnemius, vastusintermedius and vastuslateralis were
obtained from human cadaveric tissue. Serial cryosections were independently stained with anti-ANKRD2
and antibodies for different myosin heavy chain isoforms (6H1 for type 2X, BF35 for type 1 and 2A, antiMHCs for type 1 and anti-MHCf for type 2A and 2X fibers). Immunostained tissues were analyzed by fluorescent microscopy.
Results: In addition to slow, type 1, ANKRD2 wasfound expressed in fast, type 2A myofibers, which both
have oxidative metabolism. Further, we did not observe ANDRD2 expression in glycolytic, type 2X
myiofibers. This pattern of ANKRD2 expression was consistent across all examined muscles.
Conclusion: Our resultsimplicate that the regulatory mechanism of ANKRD2 expression in human skeletal muscle is associated with oxidative metabolism, rather than muscle contraction speed.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Determination of muscle fiber types expressing ANKRD2",
pages = "155-155",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2114"
}

Novković, M., Vasić, M., Jasnić, J., Milošević, E., Milovanović, M., Savić, S.,& Kojić, S.. (2023). Determination of muscle fiber types expressing ANKRD2. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 155-155.
https://hdl.handle.net/21.15107/rcub_imagine_2114

Novković M, Vasić M, Jasnić J, Milošević E, Milovanović M, Savić S, Kojić S. Determination of muscle fiber types expressing ANKRD2. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:155-155.
https://hdl.handle.net/21.15107/rcub_imagine_2114 .

Novković, Mirjana, Vasić, Marko, Jasnić, Jovana, Milošević, Emilija, Milovanović, Mina, Savić, Slobodan, Kojić, Snežana, "Determination of muscle fiber types expressing ANKRD2" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):155-155,
https://hdl.handle.net/21.15107/rcub_imagine_2114 .

TY  - DATA
AU  - Milošević, Emilija
AU  - Stanisavljević, Nemanja
AU  - Bošković, Srđan
AU  - Stamenković, Nemanja
AU  - Novković, Mirjana
AU  - Bavelloni, Alberto
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Jasnić, Jovana
PY  - 2023
UR  - https://doi.org/10.1007/s00432-023-04930-9
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1929
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1929
ER  - 

@misc{
author = "Milošević, Emilija and Stanisavljević, Nemanja and Bošković, Srđan and Stamenković, Nemanja and Novković, Mirjana and Bavelloni, Alberto and Cenni, Vittoria and Kojić, Snežana and Jasnić, Jovana",
year = "2023",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1929"
}

Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9. in Journal of Cancer Research and Clinical Oncology.
https://hdl.handle.net/21.15107/rcub_imagine_1929

Milošević E, Stanisavljević N, Bošković S, Stamenković N, Novković M, Bavelloni A, Cenni V, Kojić S, Jasnić J. Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9. in Journal of Cancer Research and Clinical Oncology. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_1929 .

Milošević, Emilija, Stanisavljević, Nemanja, Bošković, Srđan, Stamenković, Nemanja, Novković, Mirjana, Bavelloni, Alberto, Cenni, Vittoria, Kojić, Snežana, Jasnić, Jovana, "Supplementary data for the article: Milošević, E., Stanisavljević, N., Bošković, S., Stamenković, N., Novković, M., Bavelloni, A., Cenni, V., Kojić, S.,& Jasnić, J.. (2023). Antitumor activity of natural pigment violacein against osteosarcoma and rhabdomyosarcoma cell lines. in Journal of Cancer Research and Clinical Oncology. https://doi.org/10.1007/s00432-023-04930-9" in Journal of Cancer Research and Clinical Oncology (2023),
https://hdl.handle.net/21.15107/rcub_imagine_1929 .

TY  - CONF
AU  - Milošević, Emilija
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Cenni, V.
AU  - Bavelloni, A.
AU  - Kojić, Snežana
PY  - 2023
UR  - https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.13471
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1911
AB  - Introduction: Rhabdomyosarcoma (RMS) is the most common soft tissue
malignancy in children and adolescents. Respecting the age
of the patients and the tumor aggressiveness, investigation
of the molecular mechanisms of RMS tumorigenesis is
essential, most notably due to the possible identification of
novel therapeutic targets. To contribute to a better
understanding of the molecular pathology of RMS, we
investigated ANKRD1 (ankyrin repeat domain 1) gene,
considered a potential RMS diagnostic marker. The
changes in its expression are related to carcinogenesis and
resistance to chemotherapy in several types of tumors.
PB  - Wiley
C3  - Molecular oncology
T1  - Expression profiling of ANKRD1 in rhabdomyosarcoma cell lines
EP  - 197
SP  - 196
VL  - 17
VL  - Supplement 1
DO  - doi.org/10.1002/1878-0261.13471
ER  - 

@conference{
author = "Milošević, Emilija and Jasnić, Jovana and Novković, Mirjana and Cenni, V. and Bavelloni, A. and Kojić, Snežana",
year = "2023",
abstract = "Introduction: Rhabdomyosarcoma (RMS) is the most common soft tissue
malignancy in children and adolescents. Respecting the age
of the patients and the tumor aggressiveness, investigation
of the molecular mechanisms of RMS tumorigenesis is
essential, most notably due to the possible identification of
novel therapeutic targets. To contribute to a better
understanding of the molecular pathology of RMS, we
investigated ANKRD1 (ankyrin repeat domain 1) gene,
considered a potential RMS diagnostic marker. The
changes in its expression are related to carcinogenesis and
resistance to chemotherapy in several types of tumors.",
publisher = "Wiley",
journal = "Molecular oncology",
title = "Expression profiling of ANKRD1 in rhabdomyosarcoma cell lines",
pages = "197-196",
volume = "17, Supplement 1",
doi = "doi.org/10.1002/1878-0261.13471"
}

Milošević, E., Jasnić, J., Novković, M., Cenni, V., Bavelloni, A.,& Kojić, S.. (2023). Expression profiling of ANKRD1 in rhabdomyosarcoma cell lines. in Molecular oncology
Wiley., 17, 196-197.
https://doi.org/doi.org/10.1002/1878-0261.13471

Milošević E, Jasnić J, Novković M, Cenni V, Bavelloni A, Kojić S. Expression profiling of ANKRD1 in rhabdomyosarcoma cell lines. in Molecular oncology. 2023;17:196-197.
doi:doi.org/10.1002/1878-0261.13471 .

Milošević, Emilija, Jasnić, Jovana, Novković, Mirjana, Cenni, V., Bavelloni, A., Kojić, Snežana, "Expression profiling of ANKRD1 in rhabdomyosarcoma cell lines" in Molecular oncology, 17 (2023):196-197,
https://doi.org/doi.org/10.1002/1878-0261.13471 . .

TY  - CONF
AU  - Milošević, Emilija
AU  - Jasnić, Jovana
AU  - Stanisavljević, Nemanja
AU  - Cenni, Vittoria
AU  - Bavelloni, Alberto
AU  - Kojić, Snežana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2101
AB  - Investigati on of natural compounds showing specific toxicity to tumor cells aims to improve the efficacy
of available therapies. Our previous research demonstrated the cytotoxic acti vity of the bacterial pigment violacein
against rhabdomyosarcoma (RMS) cell lines. RMS is the most common soft tissue malignancy in children. In this
study, we evaluated the cytotoxicity of violacein on RMS cells in combinati on with conventi onal chemotherapeutics
doxorubicin, irinotecan, and vinflunine.
PB  - Belgrade :  Serbian Association on for Cancer Research
C3  - 6th Congress of the Serbian Association for Cancer Research (SDIR)
T1  - Violacein enhances the cytotoxic effect of commonly used chemotherapeutics on rhabdomyosarcoma cells
EP  - 94
IS  - 1
SP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2101
ER  - 

@conference{
author = "Milošević, Emilija and Jasnić, Jovana and Stanisavljević, Nemanja and Cenni, Vittoria and Bavelloni, Alberto and Kojić, Snežana",
year = "2023",
abstract = "Investigati on of natural compounds showing specific toxicity to tumor cells aims to improve the efficacy
of available therapies. Our previous research demonstrated the cytotoxic acti vity of the bacterial pigment violacein
against rhabdomyosarcoma (RMS) cell lines. RMS is the most common soft tissue malignancy in children. In this
study, we evaluated the cytotoxicity of violacein on RMS cells in combinati on with conventi onal chemotherapeutics
doxorubicin, irinotecan, and vinflunine.",
publisher = "Belgrade :  Serbian Association on for Cancer Research",
journal = "6th Congress of the Serbian Association for Cancer Research (SDIR)",
title = "Violacein enhances the cytotoxic effect of commonly used chemotherapeutics on rhabdomyosarcoma cells",
pages = "94-94",
number = "1",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2101"
}

Milošević, E., Jasnić, J., Stanisavljević, N., Cenni, V., Bavelloni, A.,& Kojić, S.. (2023). Violacein enhances the cytotoxic effect of commonly used chemotherapeutics on rhabdomyosarcoma cells. in 6th Congress of the Serbian Association for Cancer Research (SDIR)
Belgrade :  Serbian Association on for Cancer Research.(1), 94-94.
https://hdl.handle.net/21.15107/rcub_imagine_2101

Milošević E, Jasnić J, Stanisavljević N, Cenni V, Bavelloni A, Kojić S. Violacein enhances the cytotoxic effect of commonly used chemotherapeutics on rhabdomyosarcoma cells. in 6th Congress of the Serbian Association for Cancer Research (SDIR). 2023;(1):94-94.
https://hdl.handle.net/21.15107/rcub_imagine_2101 .

Milošević, Emilija, Jasnić, Jovana, Stanisavljević, Nemanja, Cenni, Vittoria, Bavelloni, Alberto, Kojić, Snežana, "Violacein enhances the cytotoxic effect of commonly used chemotherapeutics on rhabdomyosarcoma cells" in 6th Congress of the Serbian Association for Cancer Research (SDIR), no. 1 (2023):94-94,
https://hdl.handle.net/21.15107/rcub_imagine_2101 .

TY  - CONF
AU  - Kojić, Snežana
AU  - Bošković, Srđan
AU  - Milovanović, Mina
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1746
AB  - Nakon infarkta miokarda povređeno srce čoveka ne može da se regeneriše, već
reaguje formiranjem fibrotičnog ožiljka i remodelovanjem miokarda, koji dovode
do slabljenja njegove funkcije. Miokard sisara je dugo vremena smatran
postmitotičkim i terminalno diferenciranim tkivom. Međutim, kardiomiociti
sisara poseduju ograničenu sposobnost proliferacije. Njihova deoba je redak
događaj i odvija se po veoma niskoj stopi, što svakako nije dovoljno da nadoknadi
milione kardiomiocita trajno izgubljenih usled infarkta miokarda. Trenutno se
u svetu razvijaju dve strategije za podsticanje regeneracije povređenog srca
čoveka kako bi se povratile njegova struktura i funkcija. Jedna strategija
podrazumeva naseljavanje oštećenog tkiva zdravim kardiomiocitima poreklom od
indukovanih pluripotentnih ćelija, dok je cilj druge strategije aktivacija
endogenih mehanizama regeneracije. Za razliku od čoveka, neki kičmenjaci imaju
sposobnost regeneracije povređenih organa, uključujući srce. Životinja koja se
najčešće koristi za proučavanje regeneracije srca je riba zebrica (Danio rerio).
Pionirska studija o izuzetnom regenerativnom kapacitetu srca zebrice nakon
amputacije dela komore objavljena je 2002. godine, nakon koje je usledilo mnoštvo
publikacija o ćelijskim i molekularnim mehanizmima koji doprinose
regenerativnom odgovoru. Regeneracija srca zebrice je rezultat strogo regulisane
interakcije većeg broja procesa, uključujući inflamatorni odgovor,
dediferencijaciju i proliferaciju kardiomiocita, neovaskularizaciju i
reorganizaciju ekstraćelijskog matriksa. Regeneracija se može posmatrati kao
uspavan proces u organima koji ne regenerišu i manipulacijom ovog procesa bi
se mogla postići reaktivacija proliferacije u tim organima nakon povrede.
Ispitivanje interakcije između pro-regenerišućih mehanizama i procesa koji
utiču na regenerativni kapacitet treba da dovede do identifikacije faktora
potrebnih za prevazilaženje blokade regeneracije. Na taj način bi se razvile nove
strategije za indukciju proliferacije kardiomiocita i regeneraciju srca čoveka.
AB  - Након инфаркта миокарда повређено срце човека не може да се регенерише, већ
реагује формирањем фибротичног ожиљка и ремоделовањем миокарда, који доводе
до слабљења његове функције. Миокард сисара је дуго времена сматран
постмитотичким и терминално диференцираним ткивом. Међутим, кардиомиоцити
сисара поседују ограничену способност пролиферације. Њихова деоба је редак
догађај и одвија се по веома ниској стопи, што свакако није довољно да надокнади
милионе кардиомиоцита трајно изгубљених услед инфаркта миокарда. Тренутно се
у свету развијају две стратегије за подстицање регенерације повређеног срца
човека како би се повратиле његова структура и функција. Једна стратегија
подразумева насељавање оштећеног ткива здравим кардиомиоцитима пореклом од
индукованих плурипотентних ћелија, док је циљ друге стратегије активација
ендогених механизама регенерације. За разлику од човека, неки кичмењаци имају
способност регенерације повређених органа, укључујући срце. Животиња која се
најчешће користи за проучавање регенерације срца је риба зебрица (Danio rerio).
Пионирска студија о изузетном регенеративном капацитету срца зебрице након
ампутације дела коморе објављена је 2002. године, након које је уследило мноштво
публикација о ћелијским и молекуларним механизмима који доприносе
регенеративном одговору. Регенерација срца зебрице је резултат строго регулисане
интеракције већег броја процеса, укључујући инфламаторни одговор,
дедиференцијацију и пролиферацију кардиомиоцита, неоваскуларизацију и
реорганизацију екстраћелијског матрикса. Регенерација се може посматрати као
успаван процес у органима који не регенеришу и манипулацијом овог процеса би
се могла постићи реактивација пролиферације у тим органима након повреде.
Испитивање интеракције између про-регенеришућих механизама и процеса који
утичу на регенеративни капацитет треба да доведе до идентификације фактора
потребних за превазилажење блокаде регенерације. На тај начин би се развиле нове
стратегије за индукцију пролиферације кардиомиоцита и регенерацију срца човека.
PB  - Beograd : Srpsko biološko društvo
C3  - Treći kongres biologa Srbije
T1  - Mehanizmi regeneracije srca – šta možemo naučiti od zebrice
T1  - Механизми регенерације срца – шта можемо научити од зебрице
SP  - 288
UR  - https://hdl.handle.net/21.15107/rcub_imagine_1746
ER  - 

@conference{
author = "Kojić, Snežana and Bošković, Srđan and Milovanović, Mina",
year = "2022",
abstract = "Nakon infarkta miokarda povređeno srce čoveka ne može da se regeneriše, već
reaguje formiranjem fibrotičnog ožiljka i remodelovanjem miokarda, koji dovode
do slabljenja njegove funkcije. Miokard sisara je dugo vremena smatran
postmitotičkim i terminalno diferenciranim tkivom. Međutim, kardiomiociti
sisara poseduju ograničenu sposobnost proliferacije. Njihova deoba je redak
događaj i odvija se po veoma niskoj stopi, što svakako nije dovoljno da nadoknadi
milione kardiomiocita trajno izgubljenih usled infarkta miokarda. Trenutno se
u svetu razvijaju dve strategije za podsticanje regeneracije povređenog srca
čoveka kako bi se povratile njegova struktura i funkcija. Jedna strategija
podrazumeva naseljavanje oštećenog tkiva zdravim kardiomiocitima poreklom od
indukovanih pluripotentnih ćelija, dok je cilj druge strategije aktivacija
endogenih mehanizama regeneracije. Za razliku od čoveka, neki kičmenjaci imaju
sposobnost regeneracije povređenih organa, uključujući srce. Životinja koja se
najčešće koristi za proučavanje regeneracije srca je riba zebrica (Danio rerio).
Pionirska studija o izuzetnom regenerativnom kapacitetu srca zebrice nakon
amputacije dela komore objavljena je 2002. godine, nakon koje je usledilo mnoštvo
publikacija o ćelijskim i molekularnim mehanizmima koji doprinose
regenerativnom odgovoru. Regeneracija srca zebrice je rezultat strogo regulisane
interakcije većeg broja procesa, uključujući inflamatorni odgovor,
dediferencijaciju i proliferaciju kardiomiocita, neovaskularizaciju i
reorganizaciju ekstraćelijskog matriksa. Regeneracija se može posmatrati kao
uspavan proces u organima koji ne regenerišu i manipulacijom ovog procesa bi
se mogla postići reaktivacija proliferacije u tim organima nakon povrede.
Ispitivanje interakcije između pro-regenerišućih mehanizama i procesa koji
utiču na regenerativni kapacitet treba da dovede do identifikacije faktora
potrebnih za prevazilaženje blokade regeneracije. Na taj način bi se razvile nove
strategije za indukciju proliferacije kardiomiocita i regeneraciju srca čoveka., Након инфаркта миокарда повређено срце човека не може да се регенерише, већ
реагује формирањем фибротичног ожиљка и ремоделовањем миокарда, који доводе
до слабљења његове функције. Миокард сисара је дуго времена сматран
постмитотичким и терминално диференцираним ткивом. Међутим, кардиомиоцити
сисара поседују ограничену способност пролиферације. Њихова деоба је редак
догађај и одвија се по веома ниској стопи, што свакако није довољно да надокнади
милионе кардиомиоцита трајно изгубљених услед инфаркта миокарда. Тренутно се
у свету развијају две стратегије за подстицање регенерације повређеног срца
човека како би се повратиле његова структура и функција. Једна стратегија
подразумева насељавање оштећеног ткива здравим кардиомиоцитима пореклом од
индукованих плурипотентних ћелија, док је циљ друге стратегије активација
ендогених механизама регенерације. За разлику од човека, неки кичмењаци имају
способност регенерације повређених органа, укључујући срце. Животиња која се
најчешће користи за проучавање регенерације срца је риба зебрица (Danio rerio).
Пионирска студија о изузетном регенеративном капацитету срца зебрице након
ампутације дела коморе објављена је 2002. године, након које је уследило мноштво
публикација о ћелијским и молекуларним механизмима који доприносе
регенеративном одговору. Регенерација срца зебрице је резултат строго регулисане
интеракције већег броја процеса, укључујући инфламаторни одговор,
дедиференцијацију и пролиферацију кардиомиоцита, неоваскуларизацију и
реорганизацију екстраћелијског матрикса. Регенерација се може посматрати као
успаван процес у органима који не регенеришу и манипулацијом овог процеса би
се могла постићи реактивација пролиферације у тим органима након повреде.
Испитивање интеракције између про-регенеришућих механизама и процеса који
утичу на регенеративни капацитет треба да доведе до идентификације фактора
потребних за превазилажење блокаде регенерације. На тај начин би се развиле нове
стратегије за индукцију пролиферације кардиомиоцита и регенерацију срца човека.",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Treći kongres biologa Srbije",
title = "Mehanizmi regeneracije srca – šta možemo naučiti od zebrice, Механизми регенерације срца – шта можемо научити од зебрице",
pages = "288",
url = "https://hdl.handle.net/21.15107/rcub_imagine_1746"
}

Kojić, S., Bošković, S.,& Milovanović, M.. (2022). Mehanizmi regeneracije srca – šta možemo naučiti od zebrice. in Treći kongres biologa Srbije
Beograd : Srpsko biološko društvo., 288.
https://hdl.handle.net/21.15107/rcub_imagine_1746

Kojić S, Bošković S, Milovanović M. Mehanizmi regeneracije srca – šta možemo naučiti od zebrice. in Treći kongres biologa Srbije. 2022;:288.
https://hdl.handle.net/21.15107/rcub_imagine_1746 .

Kojić, Snežana, Bošković, Srđan, Milovanović, Mina, "Mehanizmi regeneracije srca – šta možemo naučiti od zebrice" in Treći kongres biologa Srbije (2022):288,
https://hdl.handle.net/21.15107/rcub_imagine_1746 .

TY  - JOUR
AU  - Stanković, Marija
AU  - Veljović, Katarina
AU  - Popović, Nikola
AU  - Kojić, Snežana
AU  - Dunjić Manevski, Sofija
AU  - Radojković, Dragica
AU  - Golić, Nataša
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1538
AB  - Bronchial epithelial cells are exposed to environmental influences, microbiota, and pathogens and also serve as a powerful effector that initiate and propagate inflammation by the release of proinflammatory mediators. Recent studies suggested that lung microbiota differ between inflammatory lung diseases and healthy lungs implicating their contribution in the modulation of lung immunity. Lactic acid bacteria (LAB) are natural inhabitants of healthy human lungs and also possess immunomodulatory effects, but so far, there are no studies investigating their anti-inflammatory potential in respiratory cells. In this study, we investigated immunomodulatory features of 21 natural LAB strains in lipopolysaccharide (LPS)-stimulated human bronchial epithelial cells (BEAS-2B). Our results show that several LAB strains reduced the expression of pro-inflammatory cytokine and chemokine genes. We also demonstrated that two LAB strains, Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22, effectively attenuated LPS-induced nuclear factor-kappa B (NF-kappa B) nuclear translocation. Moreover, BGZLS10-17 and BGPKM22 reduced the activation of p38, extracellular signal-related kinase (ERK), and c-Jun amino-terminal kinase (JNK) signaling cascade resulting in a reduction of pro-inflammatory mediator expressions in BEAS-2B cells. Collectively, the LAB strains BGZLS10-17 and BGPKM22 exhibited anti-inflammatory effects in BEAS-2B cells and could be employed to balance immune response in lungs and replenish diminished lung microbiota in chronic lung diseases.
PB  - MDPI, Basel
T2  - International Journal of Molecular Sciences
T1  - Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells
IS  - 10
VL  - 23
DO  - 10.3390/ijms23105547
ER  - 

@article{
author = "Stanković, Marija and Veljović, Katarina and Popović, Nikola and Kojić, Snežana and Dunjić Manevski, Sofija and Radojković, Dragica and Golić, Nataša",
year = "2022",
abstract = "Bronchial epithelial cells are exposed to environmental influences, microbiota, and pathogens and also serve as a powerful effector that initiate and propagate inflammation by the release of proinflammatory mediators. Recent studies suggested that lung microbiota differ between inflammatory lung diseases and healthy lungs implicating their contribution in the modulation of lung immunity. Lactic acid bacteria (LAB) are natural inhabitants of healthy human lungs and also possess immunomodulatory effects, but so far, there are no studies investigating their anti-inflammatory potential in respiratory cells. In this study, we investigated immunomodulatory features of 21 natural LAB strains in lipopolysaccharide (LPS)-stimulated human bronchial epithelial cells (BEAS-2B). Our results show that several LAB strains reduced the expression of pro-inflammatory cytokine and chemokine genes. We also demonstrated that two LAB strains, Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22, effectively attenuated LPS-induced nuclear factor-kappa B (NF-kappa B) nuclear translocation. Moreover, BGZLS10-17 and BGPKM22 reduced the activation of p38, extracellular signal-related kinase (ERK), and c-Jun amino-terminal kinase (JNK) signaling cascade resulting in a reduction of pro-inflammatory mediator expressions in BEAS-2B cells. Collectively, the LAB strains BGZLS10-17 and BGPKM22 exhibited anti-inflammatory effects in BEAS-2B cells and could be employed to balance immune response in lungs and replenish diminished lung microbiota in chronic lung diseases.",
publisher = "MDPI, Basel",
journal = "International Journal of Molecular Sciences",
title = "Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells",
number = "10",
volume = "23",
doi = "10.3390/ijms23105547"
}

Stanković, M., Veljović, K., Popović, N., Kojić, S., Dunjić Manevski, S., Radojković, D.,& Golić, N.. (2022). Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells. in International Journal of Molecular Sciences
MDPI, Basel., 23(10).
https://doi.org/10.3390/ijms23105547

Stanković M, Veljović K, Popović N, Kojić S, Dunjić Manevski S, Radojković D, Golić N. Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells. in International Journal of Molecular Sciences. 2022;23(10).
doi:10.3390/ijms23105547 .

Stanković, Marija, Veljović, Katarina, Popović, Nikola, Kojić, Snežana, Dunjić Manevski, Sofija, Radojković, Dragica, Golić, Nataša, "Lactobacillus brevis BGZLS10-17 and Lb. plantarum BGPKM22 Exhibit Anti-Inflammatory Effect by Attenuation of NF-kappa B and MAPK Signaling in Human Bronchial Epithelial Cells" in International Journal of Molecular Sciences, 23, no. 10 (2022),
https://doi.org/10.3390/ijms23105547 . .

TY  - JOUR
AU  - Marković, Sanja B.
AU  - Maciejewska, Natalia
AU  - Olszewski, Mateusz
AU  - Visnjevac, Aleksandar
AU  - Puerta, Adrian
AU  - Padron, Jose M.
AU  - Novaković, Irena
AU  - Kojić, Snežana
AU  - Fernandes, Henrique S.
AU  - Ramotowska, Sandra
AU  - Chylewska, Agnieszka
AU  - Makowski, Mariusz
AU  - Todorović, Tamara R.
AU  - Filipović, Nenad R.
PY  - 2022
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1551
AB  - The biological activity of Cd compounds has been investigated scarce since Cd has been recognized as a human carcinogen. However, the toxicity of cadmium is comparable to the toxicity of noble metals such as Pt and Pd. The paradigm of metal toxicity has been challenged suggesting that metal toxicity is not a constant property, yet it depends on many factors like the presence of appropriate ligands. Studies on anticancer activity of cadmium complexes showed that the complexation of various ligands resulted in complexes that showed better activities than approved drugs. In the present study, cadmium complexes with biologically potent thiazolyl/selenazoyl-hydrazone ligands have been prepared, and tested for their activity against different types of tumor cell models. The complexation of ligands with Cd(II) resulted in a synergistic effect. The antiproliferative activity study revealed that all complexes are more active compared to 5-fluorouracil and cisplatin. The mechanism of tumor cell growth inhibition reveal that selenium-based compounds induce cell death in T-47D (gland carci-noma) cells through apoptosis via caspase-3/7 activation. Additionally, their pro-apoptotic effect was stronger compared to etoposide and cisplatin. Nuclease activity, detected by gel electrophoresis, may be the possible mechanism of anticancer action of investigated complexes.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - European Journal of Medicinal Chemistry
T1  - Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters
VL  - 238
DO  - 10.1016/j.ejmech.2022.114449
ER  - 

@article{
author = "Marković, Sanja B. and Maciejewska, Natalia and Olszewski, Mateusz and Visnjevac, Aleksandar and Puerta, Adrian and Padron, Jose M. and Novaković, Irena and Kojić, Snežana and Fernandes, Henrique S. and Ramotowska, Sandra and Chylewska, Agnieszka and Makowski, Mariusz and Todorović, Tamara R. and Filipović, Nenad R.",
year = "2022",
abstract = "The biological activity of Cd compounds has been investigated scarce since Cd has been recognized as a human carcinogen. However, the toxicity of cadmium is comparable to the toxicity of noble metals such as Pt and Pd. The paradigm of metal toxicity has been challenged suggesting that metal toxicity is not a constant property, yet it depends on many factors like the presence of appropriate ligands. Studies on anticancer activity of cadmium complexes showed that the complexation of various ligands resulted in complexes that showed better activities than approved drugs. In the present study, cadmium complexes with biologically potent thiazolyl/selenazoyl-hydrazone ligands have been prepared, and tested for their activity against different types of tumor cell models. The complexation of ligands with Cd(II) resulted in a synergistic effect. The antiproliferative activity study revealed that all complexes are more active compared to 5-fluorouracil and cisplatin. The mechanism of tumor cell growth inhibition reveal that selenium-based compounds induce cell death in T-47D (gland carci-noma) cells through apoptosis via caspase-3/7 activation. Additionally, their pro-apoptotic effect was stronger compared to etoposide and cisplatin. Nuclease activity, detected by gel electrophoresis, may be the possible mechanism of anticancer action of investigated complexes.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "European Journal of Medicinal Chemistry",
title = "Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters",
volume = "238",
doi = "10.1016/j.ejmech.2022.114449"
}

Marković, S. B., Maciejewska, N., Olszewski, M., Visnjevac, A., Puerta, A., Padron, J. M., Novaković, I., Kojić, S., Fernandes, H. S., Ramotowska, S., Chylewska, A., Makowski, M., Todorović, T. R.,& Filipović, N. R.. (2022). Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters. in European Journal of Medicinal Chemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 238.
https://doi.org/10.1016/j.ejmech.2022.114449

Marković SB, Maciejewska N, Olszewski M, Visnjevac A, Puerta A, Padron JM, Novaković I, Kojić S, Fernandes HS, Ramotowska S, Chylewska A, Makowski M, Todorović TR, Filipović NR. Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters. in European Journal of Medicinal Chemistry. 2022;238.
doi:10.1016/j.ejmech.2022.114449 .

Marković, Sanja B., Maciejewska, Natalia, Olszewski, Mateusz, Visnjevac, Aleksandar, Puerta, Adrian, Padron, Jose M., Novaković, Irena, Kojić, Snežana, Fernandes, Henrique S., Ramotowska, Sandra, Chylewska, Agnieszka, Makowski, Mariusz, Todorović, Tamara R., Filipović, Nenad R., "Study of the anticancer potential of Cd complexes of selenazoyl-hydrazones and their sulfur isosters" in European Journal of Medicinal Chemistry, 238 (2022),
https://doi.org/10.1016/j.ejmech.2022.114449 . .

TY  - JOUR
AU  - Piazzi, Manuela
AU  - Kojić, Snežana
AU  - Capanni, Cristina
AU  - Stamenković, Nemanja
AU  - Bavelloni, Alberto
AU  - Marin, Oriano
AU  - Lattanzi, Giovanna
AU  - Blalock, William
AU  - Cenni, Vittoria
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1486
AB  - Simple Summary Osteosarcoma is a rare malignancy of bone, primarily affecting children and young adults. The main objective of this study was to identify novel therapeutic targets to fight the progression of this insidious disease. To this aim, the role of Ankrd2, a stress- and mechano- sensor protein known for being mostly expressed in muscle fibers, was analyzed in the modulation of osteosarcoma progression. By subjecting human osteosarcoma cell lines expressing or silencing Ankrd2 to several functional assays, our results demonstrated that Ankrd2 is involved in the pathogenesis of this cancer. Nonetheless, due to observations obtained by other studies in other model systems, our findings also suggest that Ankrd2 might behave as a "double-faced" cancer driver gene. Ankrd2 is a protein known for being mainly expressed in muscle fibers, where it participates in the mechanical stress response. Since both myocytes and osteoblasts are mesenchymal-derived cells, we were interested in examining the role of Ankrd2 in the progression of osteosarcoma which features a mechano-stress component. Although having been identified in many tumor-derived cell lines and -tissues, no study has yet described nor hypothesized any involvement for this protein in osteosarcoma tumorigenesis. In this paper, we report that Ankrd2 is expressed in cell lines obtained from human osteosarcoma and demonstrate a contribution by this protein in the pathogenesis of this insidious disease. Ankrd2 involvement in osteosarcoma development was evaluated in clones of Saos2, U2OS, HOS and MG63 cells stably expressing Ankrd2, through the investigation of hallmark processes of cancer cells. Interestingly, we found that exogenous expression of Ankrd2 influenced cellular growth, migration and clonogenicity in a cell line-dependent manner, whereas it was able to improve the formation of 3D spheroids in three out of four cellular models and enhanced matrix metalloproteinase (MMP) activity in all tested cell lines. Conversely, downregulation of Ankrd2 expression remarkably reduced proliferation and clonogenic potential of parental cells. As a whole, our data present Ankrd2 as a novel player in osteosarcoma development, opening up new therapeutic perspectives.
PB  - MDPI, Basel
T2  - Cancers
T1  - Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells
IS  - 2
VL  - 13
DO  - 10.3390/cancers13020174
ER  - 

@article{
author = "Piazzi, Manuela and Kojić, Snežana and Capanni, Cristina and Stamenković, Nemanja and Bavelloni, Alberto and Marin, Oriano and Lattanzi, Giovanna and Blalock, William and Cenni, Vittoria",
year = "2021",
abstract = "Simple Summary Osteosarcoma is a rare malignancy of bone, primarily affecting children and young adults. The main objective of this study was to identify novel therapeutic targets to fight the progression of this insidious disease. To this aim, the role of Ankrd2, a stress- and mechano- sensor protein known for being mostly expressed in muscle fibers, was analyzed in the modulation of osteosarcoma progression. By subjecting human osteosarcoma cell lines expressing or silencing Ankrd2 to several functional assays, our results demonstrated that Ankrd2 is involved in the pathogenesis of this cancer. Nonetheless, due to observations obtained by other studies in other model systems, our findings also suggest that Ankrd2 might behave as a "double-faced" cancer driver gene. Ankrd2 is a protein known for being mainly expressed in muscle fibers, where it participates in the mechanical stress response. Since both myocytes and osteoblasts are mesenchymal-derived cells, we were interested in examining the role of Ankrd2 in the progression of osteosarcoma which features a mechano-stress component. Although having been identified in many tumor-derived cell lines and -tissues, no study has yet described nor hypothesized any involvement for this protein in osteosarcoma tumorigenesis. In this paper, we report that Ankrd2 is expressed in cell lines obtained from human osteosarcoma and demonstrate a contribution by this protein in the pathogenesis of this insidious disease. Ankrd2 involvement in osteosarcoma development was evaluated in clones of Saos2, U2OS, HOS and MG63 cells stably expressing Ankrd2, through the investigation of hallmark processes of cancer cells. Interestingly, we found that exogenous expression of Ankrd2 influenced cellular growth, migration and clonogenicity in a cell line-dependent manner, whereas it was able to improve the formation of 3D spheroids in three out of four cellular models and enhanced matrix metalloproteinase (MMP) activity in all tested cell lines. Conversely, downregulation of Ankrd2 expression remarkably reduced proliferation and clonogenic potential of parental cells. As a whole, our data present Ankrd2 as a novel player in osteosarcoma development, opening up new therapeutic perspectives.",
publisher = "MDPI, Basel",
journal = "Cancers",
title = "Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells",
number = "2",
volume = "13",
doi = "10.3390/cancers13020174"
}

Piazzi, M., Kojić, S., Capanni, C., Stamenković, N., Bavelloni, A., Marin, O., Lattanzi, G., Blalock, W.,& Cenni, V.. (2021). Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells. in Cancers
MDPI, Basel., 13(2).
https://doi.org/10.3390/cancers13020174

Piazzi M, Kojić S, Capanni C, Stamenković N, Bavelloni A, Marin O, Lattanzi G, Blalock W, Cenni V. Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells. in Cancers. 2021;13(2).
doi:10.3390/cancers13020174 .

Piazzi, Manuela, Kojić, Snežana, Capanni, Cristina, Stamenković, Nemanja, Bavelloni, Alberto, Marin, Oriano, Lattanzi, Giovanna, Blalock, William, Cenni, Vittoria, "Ectopic Expression of Ankrd2 Affects Proliferation, Motility and Clonogenic Potential of Human Osteosarcoma Cells" in Cancers, 13, no. 2 (2021),
https://doi.org/10.3390/cancers13020174 . .

TY  - JOUR
AU  - Bošković, Srđan
AU  - Juez, Ruben Marin
AU  - Stamenković, Nemanja
AU  - Radojković, Dragica
AU  - Stainier, Didier Y. R.
AU  - Kojić, Snežana
PY  - 2021
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1425
AB  - Ankyrin repeat domain 1 (ANKRD1) is a functionally pleiotropic protein found in the nuclei and sarcomeres of cardiac and skeletal muscles, with a proposed role in linking myofibrilar stress and transcriptional regulation. Rapid upregulation of its expression in response to both physiological and pathological stress supports the involvement of ANKRD1 in muscle tissue adaptation and remodeling. However, the exact role of ANKRD1 remains poorly understood. To begin to investigate its function at higher resolution, we have generated and characterized a TgBAC(ankrd1a:EGFP) zebrafish line. This reporter line displays transgene expression in slow skeletal muscle fibers during development and exercise responsiveness in adult cardiac muscle. To better understand the role of Ankrd1a in pathological conditions in adult zebrafish, we assessed ankrd1a expression after cardiac ventricle cryoinjury and observed localized upregulation in cardiomyocytes in the border zone. We show that this expression in injured hearts is recapitulated by the TgBAC(ankrd1a:EGFP) reporter. Our results identify novel expression domains of ankrd1a and suggest an important role for Ankrd1a in the early stress response and regeneration of cardiac tissue. This new reporter line will help decipher the role of Ankrd1a in striated muscle stress response, including after cardiac injury.
PB  - Elsevier, Amsterdam
T2  - Gene
T1  - The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish
VL  - 792
DO  - 10.1016/j.gene.2021.145725
ER  - 

@article{
author = "Bošković, Srđan and Juez, Ruben Marin and Stamenković, Nemanja and Radojković, Dragica and Stainier, Didier Y. R. and Kojić, Snežana",
year = "2021",
abstract = "Ankyrin repeat domain 1 (ANKRD1) is a functionally pleiotropic protein found in the nuclei and sarcomeres of cardiac and skeletal muscles, with a proposed role in linking myofibrilar stress and transcriptional regulation. Rapid upregulation of its expression in response to both physiological and pathological stress supports the involvement of ANKRD1 in muscle tissue adaptation and remodeling. However, the exact role of ANKRD1 remains poorly understood. To begin to investigate its function at higher resolution, we have generated and characterized a TgBAC(ankrd1a:EGFP) zebrafish line. This reporter line displays transgene expression in slow skeletal muscle fibers during development and exercise responsiveness in adult cardiac muscle. To better understand the role of Ankrd1a in pathological conditions in adult zebrafish, we assessed ankrd1a expression after cardiac ventricle cryoinjury and observed localized upregulation in cardiomyocytes in the border zone. We show that this expression in injured hearts is recapitulated by the TgBAC(ankrd1a:EGFP) reporter. Our results identify novel expression domains of ankrd1a and suggest an important role for Ankrd1a in the early stress response and regeneration of cardiac tissue. This new reporter line will help decipher the role of Ankrd1a in striated muscle stress response, including after cardiac injury.",
publisher = "Elsevier, Amsterdam",
journal = "Gene",
title = "The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish",
volume = "792",
doi = "10.1016/j.gene.2021.145725"
}

Bošković, S., Juez, R. M., Stamenković, N., Radojković, D., Stainier, D. Y. R.,& Kojić, S.. (2021). The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish. in Gene
Elsevier, Amsterdam., 792.
https://doi.org/10.1016/j.gene.2021.145725

Bošković S, Juez RM, Stamenković N, Radojković D, Stainier DYR, Kojić S. The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish. in Gene. 2021;792.
doi:10.1016/j.gene.2021.145725 .

Bošković, Srđan, Juez, Ruben Marin, Stamenković, Nemanja, Radojković, Dragica, Stainier, Didier Y. R., Kojić, Snežana, "The stress responsive gene ankrd1a is dynamically regulated during skeletal muscle development and upregulated following cardiac injury in border zone cardiomyocytes in adult zebrafish" in Gene, 792 (2021),
https://doi.org/10.1016/j.gene.2021.145725 . .

TY  - JOUR
AU  - Stamenković, Nemanja
AU  - Jasnić, Jovana
AU  - Novković, Mirjana
AU  - Milošević, Emilija
AU  - Bošković, Srđan
AU  - Kojić, Ana
AU  - Popić, Kristina
AU  - Stanković, Marija
AU  - Wang, Yajun
AU  - Milenković, Sanja
AU  - Radojković, Dragica
AU  - Ma, Guada
AU  - Kojić, Snežana
PY  - 2020
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1307
AB  - Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chickenANKRD2. The chickenANKRD2coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative-glycolytic, type IIA myofibers. Association of chickenANKRD2with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests thatANKRD2is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus
EP  - 396
IS  - 4
SP  - 383
VL  - 154
DO  - 10.1007/s00418-020-01899-1
ER  - 

@article{
author = "Stamenković, Nemanja and Jasnić, Jovana and Novković, Mirjana and Milošević, Emilija and Bošković, Srđan and Kojić, Ana and Popić, Kristina and Stanković, Marija and Wang, Yajun and Milenković, Sanja and Radojković, Dragica and Ma, Guada and Kojić, Snežana",
year = "2020",
abstract = "Striated muscle signaling protein and transcriptional regulator ANKRD2 participates in myogenesis, myogenic differentiation, muscle adaptation and stress response. It is preferentially expressed in slow, oxidative fibers of mammalian skeletal muscle. In this study, we report on characterization of chickenANKRD2. The chickenANKRD2coding region contains 1002 bp and encodes a 334-amino acid protein which shares approximately 58% identity with human and mouse orthologs, mostly in the conserved region of ankyrin repeats. Comprehensive analysis of theANKRD2gene and protein expression in adult chicken demonstrated its predominant expression in red muscles of thigh and drumstick, compared to white muscle. It was not detected in heart and white pectoral muscle. Uneven expression of ANKRD2 in chicken skeletal muscles, observed by immunohistochemistry, was attributed to its selective expression in slow, oxidative, type I and fast, oxidative-glycolytic, type IIA myofibers. Association of chickenANKRD2with phenotypic differences between red and white muscles points to its potential role in the process of myofiber-type specification. In addition to expression in slow oxidative myofibers, as demonstrated for mammalian protein, chicken ANKRD2 was also detected in fast fibers with mixed oxidative and glycolytic metabolism. This finding suggests thatANKRD2is responsive to metabolic differences between types of avian myofibers and orientates future studies towards investigation of its role in molecular mechanisms of myofiber-type-specific gene expression.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus",
pages = "396-383",
number = "4",
volume = "154",
doi = "10.1007/s00418-020-01899-1"
}

Stamenković, N., Jasnić, J., Novković, M., Milošević, E., Bošković, S., Kojić, A., Popić, K., Stanković, M., Wang, Y., Milenković, S., Radojković, D., Ma, G.,& Kojić, S.. (2020). Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus. in Histochemistry and Cell Biology
Springer, New York., 154(4), 383-396.
https://doi.org/10.1007/s00418-020-01899-1

Stamenković N, Jasnić J, Novković M, Milošević E, Bošković S, Kojić A, Popić K, Stanković M, Wang Y, Milenković S, Radojković D, Ma G, Kojić S. Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus. in Histochemistry and Cell Biology. 2020;154(4):383-396.
doi:10.1007/s00418-020-01899-1 .

Stamenković, Nemanja, Jasnić, Jovana, Novković, Mirjana, Milošević, Emilija, Bošković, Srđan, Kojić, Ana, Popić, Kristina, Stanković, Marija, Wang, Yajun, Milenković, Sanja, Radojković, Dragica, Ma, Guada, Kojić, Snežana, "Cloning and expression profiling of muscle regulator ANKRD2 in domestic chickenGallus gallus" in Histochemistry and Cell Biology, 154, no. 4 (2020):383-396,
https://doi.org/10.1007/s00418-020-01899-1 . .

TY  - JOUR
AU  - Cenni, Vittoria
AU  - Kojić, Snežana
AU  - Capanni, Cristina
AU  - Faulkner, Georgine
AU  - Lattanzi, Giovanna
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1282
AB  - Ankrd2 (ankyrin repeats containing domain 2) or Arpp (ankyrin repeat, PEST sequence, and proline-rich region) is a member of the muscle ankyrin repeat protein family. Ankrd2 is mostly expressed in skeletal muscle, where it plays an intriguing role in the transcriptional response to stress induced by mechanical stimulation as well as by cellular reactive oxygen species. Our studies in myoblasts from Emery-Dreifuss muscular dystrophy 2, a LMNA-linked disease affecting skeletal and cardiac muscles, demonstrated that Ankrd2 is a lamin A-binding protein and that mutated lamins found in Emery-Dreifuss muscular dystrophy change the dynamics of Ankrd2 nuclear import, thus affecting oxidative stress response. In this review, besides describing the latest advances related to Ankrd2 studies, including novel discoveries on Ankrd2 isoform-specific functions, we report the main findings on the relationship of Ankrd2 with A-type lamins and discuss known and potential mechanisms involving defective Ankrd2-lamin A interplay in the pathogenesis of muscular laminopathies.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies
VL  - 2019
DO  - 10.1155/2019/7318796
ER  - 

@article{
author = "Cenni, Vittoria and Kojić, Snežana and Capanni, Cristina and Faulkner, Georgine and Lattanzi, Giovanna",
year = "2019",
abstract = "Ankrd2 (ankyrin repeats containing domain 2) or Arpp (ankyrin repeat, PEST sequence, and proline-rich region) is a member of the muscle ankyrin repeat protein family. Ankrd2 is mostly expressed in skeletal muscle, where it plays an intriguing role in the transcriptional response to stress induced by mechanical stimulation as well as by cellular reactive oxygen species. Our studies in myoblasts from Emery-Dreifuss muscular dystrophy 2, a LMNA-linked disease affecting skeletal and cardiac muscles, demonstrated that Ankrd2 is a lamin A-binding protein and that mutated lamins found in Emery-Dreifuss muscular dystrophy change the dynamics of Ankrd2 nuclear import, thus affecting oxidative stress response. In this review, besides describing the latest advances related to Ankrd2 studies, including novel discoveries on Ankrd2 isoform-specific functions, we report the main findings on the relationship of Ankrd2 with A-type lamins and discuss known and potential mechanisms involving defective Ankrd2-lamin A interplay in the pathogenesis of muscular laminopathies.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies",
volume = "2019",
doi = "10.1155/2019/7318796"
}

Cenni, V., Kojić, S., Capanni, C., Faulkner, G.,& Lattanzi, G.. (2019). Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2019.
https://doi.org/10.1155/2019/7318796

Cenni V, Kojić S, Capanni C, Faulkner G, Lattanzi G. Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/7318796 .

Cenni, Vittoria, Kojić, Snežana, Capanni, Cristina, Faulkner, Georgine, Lattanzi, Giovanna, "Ankrd2 in Mechanotransduction and Oxidative Stress Response in Skeletal Muscle: New Cues for the Pathogenesis of Muscular Laminopathies" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/7318796 . .

TY  - JOUR
AU  - Liang, C.
AU  - Xu, X.
AU  - Yu, H.
AU  - Zhou, X.
AU  - Wen, X.
AU  - Li, Y.
AU  - Kojić, Snežana
AU  - Wang, Y.
AU  - Ma, G.
PY  - 2019
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1229
AB  - Objective: To construct the recombinant lentiviral vector containing cardiac adriamycin responsive protein (CARP) gene and small-hairpin RNA (shRNA) targeting CARP gene and to pack the lentivirus, and to lay the foundation for further study on the function and mechanism of CARP in adriamycin (ADR) induced cardiomyopathy. Methods: After the rat CARP gene was amplified by PCR and shRNAs targeting CARP were designed and synthesized, they were inserted into the shuttle plasmids GV-358 or GV-248. respectively. After confirmed by sequencing, the recombinant shuttle vectors containing CARP gene or shRNA and auxiliary packaging plasmid Helper 1 0 and Helper 2 0 were co transfected into the IIEK293T cells for virus packaging and amplification; the viral titer was detected by end point dilution. The IIEK293T cells were infected with the recombinant lentiviruses. and the green fluoresence intensity was observed by fluorescence mircroscope. The H9C2 cells were infected with the recombinant lentivirues and divided into control group. CARP overexpression group and shRNA group; Western blotting method was used to detect the CARP expression levels in the cells in various groups. Results: The DNA sequencing results showed that the sequences of CARP overexpression and shRNA vectors were in accordance with the designed sequences. The expression of green fluorescence protein was seen under fluorescence microscope after transfection of the vectors of the IIEK293T cells. After infection of the H9C2 cells, the expression level of CARP protein in CARP overexpression group was 5. 3 times higher than that in control group ( P=0. 01); while it was down-regulated by 53% in CARP shRNA group compared with control group ( P= 0 02). Conclusion: The lentivirus expression vectors carrying CARP or shRNA targeting CARP are successfully constructed and the lentiviruses obtained could significantly interfere the expression of CARP in the II9C2 cells.
PB  - Jilin University Press
T2  - Journal of Jilin University Medicine Edition
T1  - Construction and identification of recombinant lentivirus overexpression and RNA interference vector containing cardiac adriamycin reactive protein gene
EP  - 771
IS  - 4
SP  - 766
VL  - 45
DO  - 10.13481/j.1671-587x.20190404
ER  - 

@article{
author = "Liang, C. and Xu, X. and Yu, H. and Zhou, X. and Wen, X. and Li, Y. and Kojić, Snežana and Wang, Y. and Ma, G.",
year = "2019",
abstract = "Objective: To construct the recombinant lentiviral vector containing cardiac adriamycin responsive protein (CARP) gene and small-hairpin RNA (shRNA) targeting CARP gene and to pack the lentivirus, and to lay the foundation for further study on the function and mechanism of CARP in adriamycin (ADR) induced cardiomyopathy. Methods: After the rat CARP gene was amplified by PCR and shRNAs targeting CARP were designed and synthesized, they were inserted into the shuttle plasmids GV-358 or GV-248. respectively. After confirmed by sequencing, the recombinant shuttle vectors containing CARP gene or shRNA and auxiliary packaging plasmid Helper 1 0 and Helper 2 0 were co transfected into the IIEK293T cells for virus packaging and amplification; the viral titer was detected by end point dilution. The IIEK293T cells were infected with the recombinant lentiviruses. and the green fluoresence intensity was observed by fluorescence mircroscope. The H9C2 cells were infected with the recombinant lentivirues and divided into control group. CARP overexpression group and shRNA group; Western blotting method was used to detect the CARP expression levels in the cells in various groups. Results: The DNA sequencing results showed that the sequences of CARP overexpression and shRNA vectors were in accordance with the designed sequences. The expression of green fluorescence protein was seen under fluorescence microscope after transfection of the vectors of the IIEK293T cells. After infection of the H9C2 cells, the expression level of CARP protein in CARP overexpression group was 5. 3 times higher than that in control group ( P=0. 01); while it was down-regulated by 53% in CARP shRNA group compared with control group ( P= 0 02). Conclusion: The lentivirus expression vectors carrying CARP or shRNA targeting CARP are successfully constructed and the lentiviruses obtained could significantly interfere the expression of CARP in the II9C2 cells.",
publisher = "Jilin University Press",
journal = "Journal of Jilin University Medicine Edition",
title = "Construction and identification of recombinant lentivirus overexpression and RNA interference vector containing cardiac adriamycin reactive protein gene",
pages = "771-766",
number = "4",
volume = "45",
doi = "10.13481/j.1671-587x.20190404"
}

Liang, C., Xu, X., Yu, H., Zhou, X., Wen, X., Li, Y., Kojić, S., Wang, Y.,& Ma, G.. (2019). Construction and identification of recombinant lentivirus overexpression and RNA interference vector containing cardiac adriamycin reactive protein gene. in Journal of Jilin University Medicine Edition
Jilin University Press., 45(4), 766-771.
https://doi.org/10.13481/j.1671-587x.20190404

Liang C, Xu X, Yu H, Zhou X, Wen X, Li Y, Kojić S, Wang Y, Ma G. Construction and identification of recombinant lentivirus overexpression and RNA interference vector containing cardiac adriamycin reactive protein gene. in Journal of Jilin University Medicine Edition. 2019;45(4):766-771.
doi:10.13481/j.1671-587x.20190404 .

Liang, C., Xu, X., Yu, H., Zhou, X., Wen, X., Li, Y., Kojić, Snežana, Wang, Y., Ma, G., "Construction and identification of recombinant lentivirus overexpression and RNA interference vector containing cardiac adriamycin reactive protein gene" in Journal of Jilin University Medicine Edition, 45, no. 4 (2019):766-771,
https://doi.org/10.13481/j.1671-587x.20190404 . .

TY  - JOUR
AU  - Bošković, Srđan
AU  - Marin-Juez, Ruben
AU  - Jasnić, Jovana
AU  - Reischauer, Sven
AU  - El Sammak, Hadil
AU  - Kojić, Ana
AU  - Faulkner, Georgine
AU  - Radojković, Dragica
AU  - Stainier, Didier Y. R.
AU  - Kojić, Snežana
PY  - 2018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1141
AB  - Muscle proteins with ankyrin repeats (MARPs) ANKRD1 and ANKRD2 are titin-associated proteins with a putative role as transcriptional co-regulators in striated muscle, involved in the cellular response to mechanical, oxidative and metabolic stress. Since many aspects of the biology of MARPs, particularly exact mechanisms of their action, in striated muscle are still elusive, research in this field will benefit from novel animal model system. Here we investigated the MARPs found in zebrafish for protein structure, evolutionary conservation, spatiotemporal expression profiles and response to increased muscle activity. Ankrd1 and Ankrd2 show overall moderate conservation at the protein level, more pronounced in the region of ankyrin repeats, motifs indispensable for their function. The two zebrafish genes, ankrd1a and ankrd1b, counterparts of mammalian ANKRD1/Ankrd1, have different expression profiles during first seven days of development. Mild increase of ankrd1a transcript levels was detected at 72 hpf (1.74 +/- 0.24 fold increase relative to 24 hpf time point), while ankrd1b expression was markedly upregulated from 24 hpf onward and peaked at 72 hpf (92.18 +/- 36.95 fold increase relative to 24 hpf time point). Spatially, they exhibited non-overlapping expression patterns during skeletal muscle development in trunk (ankrd1a) and tail (ankrd1b) somites. Expression of ankrd2 was barely detectable. Zebrafish MARPs, expressed at a relatively low level in adult striated muscle, were found to be responsive to endurance exercise training consisting of two bouts of 3 hours of forced swimming daily, for five consecutive days. Three hours after the last exercise bout, ankrd1a expression increased in cardiac muscle (6.19 +/- 5.05 fold change), while ankrd1b and ankrd2 were upregulated in skeletal muscle (1.97 +/- 1.05 and 1.84 +/- 0.58 fold change, respectively). This study provides the foundation to establish zebrafish as a novel in vivo model for further investigation of MARPs function in striated muscle.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise
IS  - 9
VL  - 13
DO  - 10.1371/journal.pone.0204312
ER  - 

@article{
author = "Bošković, Srđan and Marin-Juez, Ruben and Jasnić, Jovana and Reischauer, Sven and El Sammak, Hadil and Kojić, Ana and Faulkner, Georgine and Radojković, Dragica and Stainier, Didier Y. R. and Kojić, Snežana",
year = "2018",
abstract = "Muscle proteins with ankyrin repeats (MARPs) ANKRD1 and ANKRD2 are titin-associated proteins with a putative role as transcriptional co-regulators in striated muscle, involved in the cellular response to mechanical, oxidative and metabolic stress. Since many aspects of the biology of MARPs, particularly exact mechanisms of their action, in striated muscle are still elusive, research in this field will benefit from novel animal model system. Here we investigated the MARPs found in zebrafish for protein structure, evolutionary conservation, spatiotemporal expression profiles and response to increased muscle activity. Ankrd1 and Ankrd2 show overall moderate conservation at the protein level, more pronounced in the region of ankyrin repeats, motifs indispensable for their function. The two zebrafish genes, ankrd1a and ankrd1b, counterparts of mammalian ANKRD1/Ankrd1, have different expression profiles during first seven days of development. Mild increase of ankrd1a transcript levels was detected at 72 hpf (1.74 +/- 0.24 fold increase relative to 24 hpf time point), while ankrd1b expression was markedly upregulated from 24 hpf onward and peaked at 72 hpf (92.18 +/- 36.95 fold increase relative to 24 hpf time point). Spatially, they exhibited non-overlapping expression patterns during skeletal muscle development in trunk (ankrd1a) and tail (ankrd1b) somites. Expression of ankrd2 was barely detectable. Zebrafish MARPs, expressed at a relatively low level in adult striated muscle, were found to be responsive to endurance exercise training consisting of two bouts of 3 hours of forced swimming daily, for five consecutive days. Three hours after the last exercise bout, ankrd1a expression increased in cardiac muscle (6.19 +/- 5.05 fold change), while ankrd1b and ankrd2 were upregulated in skeletal muscle (1.97 +/- 1.05 and 1.84 +/- 0.58 fold change, respectively). This study provides the foundation to establish zebrafish as a novel in vivo model for further investigation of MARPs function in striated muscle.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise",
number = "9",
volume = "13",
doi = "10.1371/journal.pone.0204312"
}

Bošković, S., Marin-Juez, R., Jasnić, J., Reischauer, S., El Sammak, H., Kojić, A., Faulkner, G., Radojković, D., Stainier, D. Y. R.,& Kojić, S.. (2018). Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise. in PLoS One
Public Library Science, San Francisco., 13(9).
https://doi.org/10.1371/journal.pone.0204312

Bošković S, Marin-Juez R, Jasnić J, Reischauer S, El Sammak H, Kojić A, Faulkner G, Radojković D, Stainier DYR, Kojić S. Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise. in PLoS One. 2018;13(9).
doi:10.1371/journal.pone.0204312 .

Bošković, Srđan, Marin-Juez, Ruben, Jasnić, Jovana, Reischauer, Sven, El Sammak, Hadil, Kojić, Ana, Faulkner, Georgine, Radojković, Dragica, Stainier, Didier Y. R., Kojić, Snežana, "Characterization of zebrafish (Danio rerio) muscle ankyrin repeat proteins reveals their conserved response to endurance exercise" in PLoS One, 13, no. 9 (2018),
https://doi.org/10.1371/journal.pone.0204312 . .

TY  - JOUR
AU  - Rakocević, Jelena
AU  - Kojić, Snežana
AU  - Orlić, Dejan
AU  - Stanković, Goran
AU  - Ostojić, Miodrag
AU  - Petrović, Olga
AU  - Zaletel, Ivan
AU  - Puskas, Nela
AU  - Todorović, Vera
AU  - Labudović-Borović, Milica
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/979
AB  - Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Experimental and Molecular Pathology
T1  - Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction
EP  - 38
IS  - 1
SP  - 31
VL  - 100
DO  - 10.1016/j.yexmp.2015.11.028
ER  - 

@article{
author = "Rakocević, Jelena and Kojić, Snežana and Orlić, Dejan and Stanković, Goran and Ostojić, Miodrag and Petrović, Olga and Zaletel, Ivan and Puskas, Nela and Todorović, Vera and Labudović-Borović, Milica",
year = "2016",
abstract = "Introduction: Angiogenesis is the growth of both new vascular and lymphatic blood vessels from the existing vasculature. During this process, blood endothelial cells (BECs) and lymphatic endothelial cells (LECs) express specific markers, which help their discrimination and easier identification. Since the coronary thrombi material aspirated from patients with ST-elevation myocardial infarction (STEMI) proved as good angiogenesis model, we investigated the expression of CD34 and CD31 as BECs markers, and D2-40, LYVE-1 and VEGFR3 as LEC markers in this material. Materials and methods: Aspirated thrombi were stained immunohistochemically for CD34, CD31, D2-40, LYVE-1 and VEGFR3. Organizational patterns of immunopositive cells were graded as single cells, clusters or microvessels. Double immunofluorescence for CD31, D2-40, LYVE-1 and VEGRF3 was done. Thrombi were also graded as fresh ( lt 1 day old), lytic (1-5 days old) and organized ( gt 5 days old). Results: Serial sections of aspirated thrombi showed concordant BEC and LEC markers immunopositivity. Double immunoflorescence proved co-expression of CD31 and LEC markers on the same cells. Cells expressing LEC markers organized in clusters and microvessels were mainly present in lytic and organized thrombi. Conclusion: Co-expression of BEC and LEC markers on the same non-tumorous cell during thrombus neovascularization indicates existing in vivo plasticity of endothelial cells under non-tumorous pathological conditions. It also points that CD34 and CD31 on one hand, and D2-40, LYVE-1 and VEGFR3 immunostaining on the other hand, cannot solely be a reliable indicators whether vessel is lymphatic or not.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Experimental and Molecular Pathology",
title = "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction",
pages = "38-31",
number = "1",
volume = "100",
doi = "10.1016/j.yexmp.2015.11.028"
}

Rakocević, J., Kojić, S., Orlić, D., Stanković, G., Ostojić, M., Petrović, O., Zaletel, I., Puskas, N., Todorović, V.,& Labudović-Borović, M.. (2016). Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology
Academic Press Inc Elsevier Science, San Diego., 100(1), 31-38.
https://doi.org/10.1016/j.yexmp.2015.11.028

Rakocević J, Kojić S, Orlić D, Stanković G, Ostojić M, Petrović O, Zaletel I, Puskas N, Todorović V, Labudović-Borović M. Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction. in Experimental and Molecular Pathology. 2016;100(1):31-38.
doi:10.1016/j.yexmp.2015.11.028 .

Rakocević, Jelena, Kojić, Snežana, Orlić, Dejan, Stanković, Goran, Ostojić, Miodrag, Petrović, Olga, Zaletel, Ivan, Puskas, Nela, Todorović, Vera, Labudović-Borović, Milica, "Co-expression of vascular and lymphatic endothelial cell markers on early endothelial cells present in aspirated coronary thrombi from patients with ST-elevation myocardial infarction" in Experimental and Molecular Pathology, 100, no. 1 (2016):31-38,
https://doi.org/10.1016/j.yexmp.2015.11.028 . .

TY  - JOUR
AU  - Stanković, Marija
AU  - Kojić, Snežana
AU  - Đorđević, Valentina
AU  - Tomović, Andrija
AU  - Nagorni-Obradović, Ljudmila
AU  - Petrović-Stanojević, Nataša
AU  - Mitić-Milikić, Marija
AU  - Radojković, Dragica
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/901
AB  - The aetiology of chronic obstructive pulmonary disease (COPD) is complex. While cigarette smoking is a well-established cause of COPD, a myriad of assessed genetic factors has given conflicting data. Since gene-environment interactions are thought to be implicated in aetiopathogenesis of COPD, we aimed to examine the matrix metalloproteinase (MMP) 9 C-1562T (rs3918242) functional variant and cigarette smoke in the pathogenesis of this disease. The distribution of the MMP9 C-1562T variant was analyzed in COPD patients and controls with normal pulmonary function from Serbia. Interaction between the C-1562T genetic variant and cigarette smoking was assessed using a case-control model. The response of the C-1562T promoter variant to cigarette smoke condensate (CSC) exposure was examined using a dual luciferase reporter assay. The frequency of T allele carriers was higher in the COPD group than in smoker controls (38.4% vs. 20%; OR=2.7, P=0.027). Interaction between the T allele and cigarette smoking was identified in COPD occurrence (OR=4.38, P=0.005) and severity (P=0.001). A functional analysis of the C-1562T variant demonstrated a dose-dependent and allele-specific response (P lt 0.01) to CSC. Significantly higher MMP9 promoter activity following CSC exposure was found for the promoter harboring the T allele compared to the promoter harboring the C allele (P lt 0.05). Our study is the first to reveal an interaction between the MMP9-1562T allele and cigarette smoke in COPD, emphasising gene-environment interactions as a possible cause of lung damage in the pathogenesis of COPD. Environ. Mol. Mutagen. 57:447-454, 2016.
PB  - Wiley, Hoboken
T2  - Environmental and Molecular Mutagenesis
T1  - Gene-environment interaction between the MMP9 C-1562T promoter variant and cigarette smoke in the pathogenesis of chronic obstructive pulmonary disease
EP  - 454
IS  - 6
SP  - 447
VL  - 57
DO  - 10.1002/em.22025
ER  - 

@article{
author = "Stanković, Marija and Kojić, Snežana and Đorđević, Valentina and Tomović, Andrija and Nagorni-Obradović, Ljudmila and Petrović-Stanojević, Nataša and Mitić-Milikić, Marija and Radojković, Dragica",
year = "2016",
abstract = "The aetiology of chronic obstructive pulmonary disease (COPD) is complex. While cigarette smoking is a well-established cause of COPD, a myriad of assessed genetic factors has given conflicting data. Since gene-environment interactions are thought to be implicated in aetiopathogenesis of COPD, we aimed to examine the matrix metalloproteinase (MMP) 9 C-1562T (rs3918242) functional variant and cigarette smoke in the pathogenesis of this disease. The distribution of the MMP9 C-1562T variant was analyzed in COPD patients and controls with normal pulmonary function from Serbia. Interaction between the C-1562T genetic variant and cigarette smoking was assessed using a case-control model. The response of the C-1562T promoter variant to cigarette smoke condensate (CSC) exposure was examined using a dual luciferase reporter assay. The frequency of T allele carriers was higher in the COPD group than in smoker controls (38.4% vs. 20%; OR=2.7, P=0.027). Interaction between the T allele and cigarette smoking was identified in COPD occurrence (OR=4.38, P=0.005) and severity (P=0.001). A functional analysis of the C-1562T variant demonstrated a dose-dependent and allele-specific response (P lt 0.01) to CSC. Significantly higher MMP9 promoter activity following CSC exposure was found for the promoter harboring the T allele compared to the promoter harboring the C allele (P lt 0.05). Our study is the first to reveal an interaction between the MMP9-1562T allele and cigarette smoke in COPD, emphasising gene-environment interactions as a possible cause of lung damage in the pathogenesis of COPD. Environ. Mol. Mutagen. 57:447-454, 2016.",
publisher = "Wiley, Hoboken",
journal = "Environmental and Molecular Mutagenesis",
title = "Gene-environment interaction between the MMP9 C-1562T promoter variant and cigarette smoke in the pathogenesis of chronic obstructive pulmonary disease",
pages = "454-447",
number = "6",
volume = "57",
doi = "10.1002/em.22025"
}

Stanković, M., Kojić, S., Đorđević, V., Tomović, A., Nagorni-Obradović, L., Petrović-Stanojević, N., Mitić-Milikić, M.,& Radojković, D.. (2016). Gene-environment interaction between the MMP9 C-1562T promoter variant and cigarette smoke in the pathogenesis of chronic obstructive pulmonary disease. in Environmental and Molecular Mutagenesis
Wiley, Hoboken., 57(6), 447-454.
https://doi.org/10.1002/em.22025

Stanković M, Kojić S, Đorđević V, Tomović A, Nagorni-Obradović L, Petrović-Stanojević N, Mitić-Milikić M, Radojković D. Gene-environment interaction between the MMP9 C-1562T promoter variant and cigarette smoke in the pathogenesis of chronic obstructive pulmonary disease. in Environmental and Molecular Mutagenesis. 2016;57(6):447-454.
doi:10.1002/em.22025 .

Stanković, Marija, Kojić, Snežana, Đorđević, Valentina, Tomović, Andrija, Nagorni-Obradović, Ljudmila, Petrović-Stanojević, Nataša, Mitić-Milikić, Marija, Radojković, Dragica, "Gene-environment interaction between the MMP9 C-1562T promoter variant and cigarette smoke in the pathogenesis of chronic obstructive pulmonary disease" in Environmental and Molecular Mutagenesis, 57, no. 6 (2016):447-454,
https://doi.org/10.1002/em.22025 . .

TY  - JOUR
AU  - Jasnić, Jovana
AU  - Krause, Sabine
AU  - Savić, Slobodan
AU  - Kojić, Ana
AU  - Kovcić, Vlado
AU  - Bošković, Srđan
AU  - Nestorović, Aleksandra
AU  - Rakićević, Ljiljana
AU  - Schreiber-Katz, Olivia
AU  - Vogel, Johannes G.
AU  - Schoser, Benedikt G.
AU  - Walter, Maggie C.
AU  - Valle, Giorgio
AU  - Radojković, Dragica
AU  - Faulkner, Georgine
AU  - Kojić, Snežana
PY  - 2016
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/933
AB  - Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles
EP  - 584
IS  - 5
SP  - 569
VL  - 146
DO  - 10.1007/s00418-016-1465-0
ER  - 

@article{
author = "Jasnić, Jovana and Krause, Sabine and Savić, Slobodan and Kojić, Ana and Kovcić, Vlado and Bošković, Srđan and Nestorović, Aleksandra and Rakićević, Ljiljana and Schreiber-Katz, Olivia and Vogel, Johannes G. and Schoser, Benedikt G. and Walter, Maggie C. and Valle, Giorgio and Radojković, Dragica and Faulkner, Georgine and Kojić, Snežana",
year = "2016",
abstract = "Four human Ankrd2 transcripts, reported in the Ensembl database, code for distinct protein isoforms (360, 333, 327 and 300 aa), and so far, their existence, specific expression and localization patterns have not been studied in detail. Ankrd2 is preferentially expressed in the slow fibers of skeletal muscle. It is found in both the nuclei and the cytoplasm of skeletal muscle cells, and its localization is prone to change during differentiation and upon stress. Ankrd2 has also been detected in the heart, in ventricular cardiomyocytes and in the intercalated disks (ICDs). The main objective of this study was to distinguish between the Ankrd2 isoforms and to determine the contribution of each one to the general profile of Ankrd2 expression in striated muscles. We demonstrated that the known expression and localization pattern of Ankrd2 in striated muscle can be attributed to the isoform of 333 aa which is dominant in both tissues, while the designated cardiac and canonical isoform of 360 aa was less expressed in both tissues. The 360 aa isoform has a distinct nuclear localization in human skeletal muscle, as well as in primary myoblasts and myotubes. In contrast to the isoform of 333 aa, it was not preferentially expressed in slow fibers and not localized to the ICDs of human cardiomyocytes. Regulation of the expression of both isoforms is achieved at the transcriptional level. Our results set the stage for investigation of the specific functions and interactions of the Ankrd2 isoforms in healthy and diseased human striated muscles.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles",
pages = "584-569",
number = "5",
volume = "146",
doi = "10.1007/s00418-016-1465-0"
}

Jasnić, J., Krause, S., Savić, S., Kojić, A., Kovcić, V., Bošković, S., Nestorović, A., Rakićević, L., Schreiber-Katz, O., Vogel, J. G., Schoser, B. G., Walter, M. C., Valle, G., Radojković, D., Faulkner, G.,& Kojić, S.. (2016). Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles. in Histochemistry and Cell Biology
Springer, New York., 146(5), 569-584.
https://doi.org/10.1007/s00418-016-1465-0

Jasnić J, Krause S, Savić S, Kojić A, Kovcić V, Bošković S, Nestorović A, Rakićević L, Schreiber-Katz O, Vogel JG, Schoser BG, Walter MC, Valle G, Radojković D, Faulkner G, Kojić S. Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles. in Histochemistry and Cell Biology. 2016;146(5):569-584.
doi:10.1007/s00418-016-1465-0 .

Jasnić, Jovana, Krause, Sabine, Savić, Slobodan, Kojić, Ana, Kovcić, Vlado, Bošković, Srđan, Nestorović, Aleksandra, Rakićević, Ljiljana, Schreiber-Katz, Olivia, Vogel, Johannes G., Schoser, Benedikt G., Walter, Maggie C., Valle, Giorgio, Radojković, Dragica, Faulkner, Georgine, Kojić, Snežana, "Differential expression and localization of Ankrd2 isoforms in human skeletal and cardiac muscles" in Histochemistry and Cell Biology, 146, no. 5 (2016):569-584,
https://doi.org/10.1007/s00418-016-1465-0 . .

TY  - JOUR
AU  - Tesić, Vesna
AU  - Perović, Milka
AU  - Lazić, Divna
AU  - Kojić, Snežana
AU  - Smiljanić, Kosara
AU  - Ruzdijić, Sabera
AU  - Rakić, Ljubisav
AU  - Kanazir, Selma
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/880
AB  - Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results reveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Journal of Steroid Biochemistry and Molecular Biology
T1  - Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat
EP  - 52
SP  - 43
VL  - 149
DO  - 10.1016/j.jsbmb.2015.01.013
ER  - 

@article{
author = "Tesić, Vesna and Perović, Milka and Lazić, Divna and Kojić, Snežana and Smiljanić, Kosara and Ruzdijić, Sabera and Rakić, Ljubisav and Kanazir, Selma",
year = "2015",
abstract = "Diminished glucocorticoid signaling is associated with an age-related decline in hippocampal functioning. In this study we demonstrate the effect of intermittent, every other day (EOD) feeding on the glucocorticoid hormone/glucocorticoid receptor (GR) system in the hippocampus of middle-aged (18-month-old) and aged (24-month-old) Wistar rats. In aged ad libitum-fed rats, a decrease in the level of total GR and GR phosphorylated at Ser(232) (pGR) was detected. Conversely, aged rats subjected to EOD feeding, starting from 6 months of age, showed an increase in GR and pGR levels and a higher content of hippocampal corticosterone. Furthermore, prominent nuclear staining of pGR was observed in CM pyramidal and DG granule neurons of aged EOD-fed rats. These changes were accompanied by increased Sglc-1 and decreased GFAP transcription, pointing to upregulated transcriptional activity of GR. EOD feeding also induced an increase in the expression of the mineralocorticoid receptor. Our results reveal that intermittent feeding restores impaired GR signaling in the hippocampus of aged animals by inducing rather than by stabilizing GR signaling during aging.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Journal of Steroid Biochemistry and Molecular Biology",
title = "Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat",
pages = "52-43",
volume = "149",
doi = "10.1016/j.jsbmb.2015.01.013"
}

Tesić, V., Perović, M., Lazić, D., Kojić, S., Smiljanić, K., Ruzdijić, S., Rakić, L.,& Kanazir, S.. (2015). Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. in Journal of Steroid Biochemistry and Molecular Biology
Pergamon-Elsevier Science Ltd, Oxford., 149, 43-52.
https://doi.org/10.1016/j.jsbmb.2015.01.013

Tesić V, Perović M, Lazić D, Kojić S, Smiljanić K, Ruzdijić S, Rakić L, Kanazir S. Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat. in Journal of Steroid Biochemistry and Molecular Biology. 2015;149:43-52.
doi:10.1016/j.jsbmb.2015.01.013 .

Tesić, Vesna, Perović, Milka, Lazić, Divna, Kojić, Snežana, Smiljanić, Kosara, Ruzdijić, Sabera, Rakić, Ljubisav, Kanazir, Selma, "Long-term intermittent feeding restores impaired GR signaling in the hippocampus of aged rat" in Journal of Steroid Biochemistry and Molecular Biology, 149 (2015):43-52,
https://doi.org/10.1016/j.jsbmb.2015.01.013 . .

TY  - JOUR
AU  - Jasnić, Jovana
AU  - Nestorović, Aleksandra
AU  - Savić, Slobodan
AU  - Karasek, Sinisa
AU  - Vitulo, Nicola
AU  - Valle, Giorgio
AU  - Faulkner, Georgine
AU  - Radojković, Dragica
AU  - Kojić, Snežana
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/814
AB  - Muscle-specific mechanosensors Ankrd2/Arpp (ankyrin repeat protein 2) and Ankrd1/CARP (cardiac ankyrin repeat protein) have an important role in transcriptional regulation, myofibrillar assembly, cardiogenesis and myogenesis. In skeletal muscle myofibrils, Ankrd2 has a structural role as a component of a titin associated stretch-sensing complex, while in the nucleus it exerts regulatory function as transcriptional co-factor. It is also involved in myogenic differentiation and coordination of myoblast proliferation. Although expressed in the heart, the role of Ankrd2 in the cardiac muscle is completely unknown. Recently, we have shown that hypertrophic and dilated cardiomyopathy pathways are altered upon Ankrd2 silencing suggesting the importance of this protein in cardiac tissue. Here we provide the underlying basis for the functional investigation of Ankrd2 in the heart. We confirmed reduced Ankrd2 expression levels in human heart in comparison with Ankrd1 using RNAseq and Western blot. For the first time we demonstrated that, apart from the sarcomere and nucleus, both proteins are localized to the intercalated disks of human cardiomyocytes. We further tested the expression and localization of endogenous Ankrd2 in rat neonatal cardiomyocytes, a well-established model for studying cardiac-specific proteins. Ankrd2 was found to be expressed in both the cytoplasm and nucleus, independently from maturation status of cardiomyocytes. In contrast to Ankrd1, it is not responsive to the cardiotoxic drug Doxorubicin, suggesting that different mechanisms govern their expression in cardiac cells.
PB  - Springer, New York
T2  - Histochemistry and Cell Biology
T1  - Profiling of skeletal muscle Ankrd2 protein in human cardiac tissue and neonatal rat cardiomyocytes
EP  - 597
IS  - 6
SP  - 583
VL  - 143
DO  - 10.1007/s00418-015-1307-5
ER  - 

@article{
author = "Jasnić, Jovana and Nestorović, Aleksandra and Savić, Slobodan and Karasek, Sinisa and Vitulo, Nicola and Valle, Giorgio and Faulkner, Georgine and Radojković, Dragica and Kojić, Snežana",
year = "2015",
abstract = "Muscle-specific mechanosensors Ankrd2/Arpp (ankyrin repeat protein 2) and Ankrd1/CARP (cardiac ankyrin repeat protein) have an important role in transcriptional regulation, myofibrillar assembly, cardiogenesis and myogenesis. In skeletal muscle myofibrils, Ankrd2 has a structural role as a component of a titin associated stretch-sensing complex, while in the nucleus it exerts regulatory function as transcriptional co-factor. It is also involved in myogenic differentiation and coordination of myoblast proliferation. Although expressed in the heart, the role of Ankrd2 in the cardiac muscle is completely unknown. Recently, we have shown that hypertrophic and dilated cardiomyopathy pathways are altered upon Ankrd2 silencing suggesting the importance of this protein in cardiac tissue. Here we provide the underlying basis for the functional investigation of Ankrd2 in the heart. We confirmed reduced Ankrd2 expression levels in human heart in comparison with Ankrd1 using RNAseq and Western blot. For the first time we demonstrated that, apart from the sarcomere and nucleus, both proteins are localized to the intercalated disks of human cardiomyocytes. We further tested the expression and localization of endogenous Ankrd2 in rat neonatal cardiomyocytes, a well-established model for studying cardiac-specific proteins. Ankrd2 was found to be expressed in both the cytoplasm and nucleus, independently from maturation status of cardiomyocytes. In contrast to Ankrd1, it is not responsive to the cardiotoxic drug Doxorubicin, suggesting that different mechanisms govern their expression in cardiac cells.",
publisher = "Springer, New York",
journal = "Histochemistry and Cell Biology",
title = "Profiling of skeletal muscle Ankrd2 protein in human cardiac tissue and neonatal rat cardiomyocytes",
pages = "597-583",
number = "6",
volume = "143",
doi = "10.1007/s00418-015-1307-5"
}

Jasnić, J., Nestorović, A., Savić, S., Karasek, S., Vitulo, N., Valle, G., Faulkner, G., Radojković, D.,& Kojić, S.. (2015). Profiling of skeletal muscle Ankrd2 protein in human cardiac tissue and neonatal rat cardiomyocytes. in Histochemistry and Cell Biology
Springer, New York., 143(6), 583-597.
https://doi.org/10.1007/s00418-015-1307-5

Jasnić J, Nestorović A, Savić S, Karasek S, Vitulo N, Valle G, Faulkner G, Radojković D, Kojić S. Profiling of skeletal muscle Ankrd2 protein in human cardiac tissue and neonatal rat cardiomyocytes. in Histochemistry and Cell Biology. 2015;143(6):583-597.
doi:10.1007/s00418-015-1307-5 .

Jasnić, Jovana, Nestorović, Aleksandra, Savić, Slobodan, Karasek, Sinisa, Vitulo, Nicola, Valle, Giorgio, Faulkner, Georgine, Radojković, Dragica, Kojić, Snežana, "Profiling of skeletal muscle Ankrd2 protein in human cardiac tissue and neonatal rat cardiomyocytes" in Histochemistry and Cell Biology, 143, no. 6 (2015):583-597,
https://doi.org/10.1007/s00418-015-1307-5 . .

TY  - JOUR
AU  - Josić, D.
AU  - Starović, M.
AU  - Kojić, Snežana
AU  - Pivić, R.
AU  - Stanojković-Sebić, A.
AU  - Zdravković, M.
AU  - Pavlović, S.
PY  - 2015
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/819
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia
EP  - 283
IS  - 2
SP  - 283
VL  - 99
DO  - 10.1094/PDIS-08-14-0875-PDN
ER  - 

@article{
author = "Josić, D. and Starović, M. and Kojić, Snežana and Pivić, R. and Stanojković-Sebić, A. and Zdravković, M. and Pavlović, S.",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia",
pages = "283-283",
number = "2",
volume = "99",
doi = "10.1094/PDIS-08-14-0875-PDN"
}

Josić, D., Starović, M., Kojić, S., Pivić, R., Stanojković-Sebić, A., Zdravković, M.,& Pavlović, S.. (2015). Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(2), 283-283.
https://doi.org/10.1094/PDIS-08-14-0875-PDN

Josić D, Starović M, Kojić S, Pivić R, Stanojković-Sebić A, Zdravković M, Pavlović S. Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia. in Plant Disease. 2015;99(2):283-283.
doi:10.1094/PDIS-08-14-0875-PDN .

Josić, D., Starović, M., Kojić, Snežana, Pivić, R., Stanojković-Sebić, A., Zdravković, M., Pavlović, S., "Dianthus barbatus-A New Host of Stolbur Phytoplasma in Serbia" in Plant Disease, 99, no. 2 (2015):283-283,
https://doi.org/10.1094/PDIS-08-14-0875-PDN . .