TY  - JOUR
AU  - Makryniotis, Konstantinos
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2345
AB  - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
T2  - Biotechnology Journal
T1  - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
IS  - n/a
SP  - 2400053
VL  - n/a
DO  - 10.1002/biot.202400053
ER  - 

@article{
author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.",
journal = "Biotechnology Journal",
title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases",
number = "n/a",
pages = "2400053",
volume = "n/a",
doi = "10.1002/biot.202400053"
}

Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E..Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053.
https://doi.org/10.1002/biot.202400053

Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal.n/a(n/a):2400053.
doi:10.1002/biot.202400053 .

Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a:2400053,
https://doi.org/10.1002/biot.202400053 . .

TY  - JOUR
AU  - Makryniotis, Konstantinos
AU  - Nikolaivits, Efstratios
AU  - Taxeidis, George
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/biot.202400053
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2341
AB  - The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.
T2  - Biotechnology Journal
T2  - Biotechnology Journal
T1  - Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases
IS  - n/a
SP  - 2400053
VL  - n/a
DO  - 10.1002/biot.202400053
ER  - 

@article{
author = "Makryniotis, Konstantinos and Nikolaivits, Efstratios and Taxeidis, George and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
abstract = "The rapid escalation of plastic waste accumulation presents a significant threat of the modern world, demanding an immediate solution. Over the last years, utilization of the enzymatic machinery of various microorganisms has emerged as an environmentally friendly asset in tackling this pressing global challenge. Thus, various hydrolases have been demonstrated to effectively degrade polyesters. Plastic waste streams often consist of a variety of different polyesters, as impurities, mainly due to wrong disposal practices, rendering recycling process challenging. The elucidation of the selective degradation of polyesters by hydrolases could offer a proper solution to this problem, enhancing the recyclability performance. Towards this, our study focused on the investigation of four bacterial polyesterases, including DaPUase, IsPETase, PfPHOase, and Se1JFR, a novel PETase-like lipase. The enzymes, which were biochemically characterized and structurally analyzed, demonstrated degradation ability of synthetic plastics. While a consistent pattern of polyesters’ degradation was observed across all enzymes, Se1JFR stood out in the degradation of PBS, PLA, and polyether PU. Additionally, it exhibited comparable results to IsPETase, a benchmark mesophilic PETase, in the degradation of PCL and semi-crystalline PET. Our results point out the wide substrate spectrum of bacterial hydrolases and underscore the significant potential of PETase-like enzymes in polyesters degradation.",
journal = "Biotechnology Journal, Biotechnology Journal",
title = "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases",
number = "n/a",
pages = "2400053",
volume = "n/a",
doi = "10.1002/biot.202400053"
}

Makryniotis, K., Nikolaivits, E., Taxeidis, G., Nikodinović-Runić, J.,& Topakas, E..Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal, n/a(n/a), 2400053.
https://doi.org/10.1002/biot.202400053

Makryniotis K, Nikolaivits E, Taxeidis G, Nikodinović-Runić J, Topakas E. Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases. in Biotechnology Journal.n/a(n/a):2400053.
doi:10.1002/biot.202400053 .

Makryniotis, Konstantinos, Nikolaivits, Efstratios, Taxeidis, George, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Exploring the substrate spectrum of phylogenetically distinct bacterial polyesterases" in Biotechnology Journal, n/a, no. n/a:2400053,
https://doi.org/10.1002/biot.202400053 . .

TY  - JOUR
AU  - Karagüzel, Ayşe
AU  - Uğur, Sümeyye Buran
AU  - Çetinkaya, Yasin
AU  - Doğan, Şengül Dilem
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
AU  - Gündüz, Miyase Gözde
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0022286024003107
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2317
AB  - Nonsteroidal anti-inflammatory drugs (NSAIDs) alleviate inflammation and pain through the inhibition of cyclooxygenase (COX) enzymes. Besides these widely recognized therapeutic utilizations, NSAIDs have been reported to display moderate antimicrobial activity and enhance antimicrobial efficacy when administered in combination with commercial antimicrobial drugs. In the present study, we designed novel potential antimicrobial agents by linking some NSAIDs (ibuprofen, flurbiprofen, and naproxen) to various azole rings (pyrazole, imidazole, triazole, and benzimidazole) via hydrazone functionality. The hydrazone linker was introduced into the chemical scaffold of the title molecules by the reaction between hydrazides obtained from NSAIDs and in-house synthesized azole-carrying benzaldehydes. The structures of the target compounds were elucidated by a combination of spectral methods. The NOESY spectra and stereochemical analyses performed using DFT method confirmed the presence of the target molecules as a mixture of E(C=N)-E(N-N)-synperiplanar and E(C=N)-E(N-N)-antiperiplanar conformers in DMSO-d6 solution. 1H and 13C NMR chemical shift values in DMSO were calculated using the GIAO method and compared with the experimental NMR data. Finally, some derivatives were demonstrated to inhibit Candida albicans filamentation and/or bacterial communication system known as quorum sensing. For COX inhibitor-azole hybrids with antimicrobial potency, naproxen appeared to be the most appropriate NSAID, while bulky benzimidazole was not found as a preferable azole ring.
PB  - Elsevier
T2  - Journal of Molecular Structure
T2  - Journal of Molecular StructureJournal of Molecular Structure
T1  - Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity
SP  - 137787
DO  - 10.1016/j.molstruc.2024.137787
ER  - 

@article{
author = "Karagüzel, Ayşe and Uğur, Sümeyye Buran and Çetinkaya, Yasin and Doğan, Şengül Dilem and Stevanović, Milena and Nikodinović-Runić, Jasmina and Gündüz, Miyase Gözde",
year = "2024",
abstract = "Nonsteroidal anti-inflammatory drugs (NSAIDs) alleviate inflammation and pain through the inhibition of cyclooxygenase (COX) enzymes. Besides these widely recognized therapeutic utilizations, NSAIDs have been reported to display moderate antimicrobial activity and enhance antimicrobial efficacy when administered in combination with commercial antimicrobial drugs. In the present study, we designed novel potential antimicrobial agents by linking some NSAIDs (ibuprofen, flurbiprofen, and naproxen) to various azole rings (pyrazole, imidazole, triazole, and benzimidazole) via hydrazone functionality. The hydrazone linker was introduced into the chemical scaffold of the title molecules by the reaction between hydrazides obtained from NSAIDs and in-house synthesized azole-carrying benzaldehydes. The structures of the target compounds were elucidated by a combination of spectral methods. The NOESY spectra and stereochemical analyses performed using DFT method confirmed the presence of the target molecules as a mixture of E(C=N)-E(N-N)-synperiplanar and E(C=N)-E(N-N)-antiperiplanar conformers in DMSO-d6 solution. 1H and 13C NMR chemical shift values in DMSO were calculated using the GIAO method and compared with the experimental NMR data. Finally, some derivatives were demonstrated to inhibit Candida albicans filamentation and/or bacterial communication system known as quorum sensing. For COX inhibitor-azole hybrids with antimicrobial potency, naproxen appeared to be the most appropriate NSAID, while bulky benzimidazole was not found as a preferable azole ring.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure, Journal of Molecular StructureJournal of Molecular Structure",
title = "Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity",
pages = "137787",
doi = "10.1016/j.molstruc.2024.137787"
}

Karagüzel, A., Uğur, S. B., Çetinkaya, Y., Doğan, Ş. D., Stevanović, M., Nikodinović-Runić, J.,& Gündüz, M. G.. (2024). Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity. in Journal of Molecular Structure
Elsevier., 137787.
https://doi.org/10.1016/j.molstruc.2024.137787

Karagüzel A, Uğur SB, Çetinkaya Y, Doğan ŞD, Stevanović M, Nikodinović-Runić J, Gündüz MG. Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity. in Journal of Molecular Structure. 2024;:137787.
doi:10.1016/j.molstruc.2024.137787 .

Karagüzel, Ayşe, Uğur, Sümeyye Buran, Çetinkaya, Yasin, Doğan, Şengül Dilem, Stevanović, Milena, Nikodinović-Runić, Jasmina, Gündüz, Miyase Gözde, "Azole rings linked to COX inhibitors via hydrazone bridge: Synthesis, stereochemical analysis, and investigation of antimicrobial activity" in Journal of Molecular Structure (2024):137787,
https://doi.org/10.1016/j.molstruc.2024.137787 . .

TY  - JOUR
AU  - Taxeidis, George
AU  - Đapović, Milica
AU  - Nikolaivits, Efstratios
AU  - Maslak, Veselin
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2024
UR  - https://doi.org/10.1021/acssuschemeng.4c00143
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2339
AB  - The discovery and engineering of novel biocatalysts capable of depolymerizing polyethylene terephthalate (PET) have gained significant attention since the need for green technologies to combat plastic pollution has become increasingly urgent. This study focuses on the development of novel substrates that can indicate enzymes with PET hydrolytic activity, streamlining the process of enzyme evaluation and selection. Four novel substrates, mimicking the structure of PET, were chemically synthesized and labeled with fluorogenic or chromogenic moieties, enabling the direct analysis of candidate enzymes without complex preparatory or analysis steps. The fluorogenic substrates, mUPET1, mUPET2, and mUPET3, not only identify enzymes capable of PET breakdown but also differentiate those with exceptional performance on the polymer, such as the benchmark PETase, LCCICCG. Among the substrates, the chromogenic p-NPhPET3 stands out as a reliable tool for screening both pure and crude enzymes, offering advantages over fluorogenic substrates such as ease of assay using UV–vis spectroscopy and compatibility with crude enzyme samples. However, ferulic acid esterases and mono-(2-hydroxyethyl) terephthalate esterases (MHETases), which exhibit remarkably high affinity for PET oligomers, also show high catalytic activity on these substrates. The substrates introduced in this study hold significant value in the function-based screening and characterization of enzymes that degrade PET, as well as the the potential to be used in screening mutant libraries derived from directed evolution experiments. Following this approach, a rapid and dependable assay method can be carried out using basic laboratory infrastructure, eliminating the necessity for intricate preparatory procedures before analysis.
PB  - American Chemical Society
T2  - ACS Sustainable Chemistry & Engineering
T1  - New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes
DO  - 10.1021/acssuschemeng.4c00143
ER  - 

@article{
author = "Taxeidis, George and Đapović, Milica and Nikolaivits, Efstratios and Maslak, Veselin and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2024",
abstract = "The discovery and engineering of novel biocatalysts capable of depolymerizing polyethylene terephthalate (PET) have gained significant attention since the need for green technologies to combat plastic pollution has become increasingly urgent. This study focuses on the development of novel substrates that can indicate enzymes with PET hydrolytic activity, streamlining the process of enzyme evaluation and selection. Four novel substrates, mimicking the structure of PET, were chemically synthesized and labeled with fluorogenic or chromogenic moieties, enabling the direct analysis of candidate enzymes without complex preparatory or analysis steps. The fluorogenic substrates, mUPET1, mUPET2, and mUPET3, not only identify enzymes capable of PET breakdown but also differentiate those with exceptional performance on the polymer, such as the benchmark PETase, LCCICCG. Among the substrates, the chromogenic p-NPhPET3 stands out as a reliable tool for screening both pure and crude enzymes, offering advantages over fluorogenic substrates such as ease of assay using UV–vis spectroscopy and compatibility with crude enzyme samples. However, ferulic acid esterases and mono-(2-hydroxyethyl) terephthalate esterases (MHETases), which exhibit remarkably high affinity for PET oligomers, also show high catalytic activity on these substrates. The substrates introduced in this study hold significant value in the function-based screening and characterization of enzymes that degrade PET, as well as the the potential to be used in screening mutant libraries derived from directed evolution experiments. Following this approach, a rapid and dependable assay method can be carried out using basic laboratory infrastructure, eliminating the necessity for intricate preparatory procedures before analysis.",
publisher = "American Chemical Society",
journal = "ACS Sustainable Chemistry & Engineering",
title = "New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes",
doi = "10.1021/acssuschemeng.4c00143"
}

Taxeidis, G., Đapović, M., Nikolaivits, E., Maslak, V., Nikodinović-Runić, J.,& Topakas, E.. (2024). New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes. in ACS Sustainable Chemistry & Engineering
American Chemical Society..
https://doi.org/10.1021/acssuschemeng.4c00143

Taxeidis G, Đapović M, Nikolaivits E, Maslak V, Nikodinović-Runić J, Topakas E. New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes. in ACS Sustainable Chemistry & Engineering. 2024;.
doi:10.1021/acssuschemeng.4c00143 .

Taxeidis, George, Đapović, Milica, Nikolaivits, Efstratios, Maslak, Veselin, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "New Labeled PET Analogues Enable the Functional Screening and Characterization of PET-Degrading Enzymes" in ACS Sustainable Chemistry & Engineering (2024),
https://doi.org/10.1021/acssuschemeng.4c00143 . .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Siaperas, Romanos
AU  - Budin, Clémence
AU  - de Boer, Tjalf
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/1751-7915.14445
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2337
AB  - Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.
PB  - Wiley
T2  - Microbial Biotechnology
T2  - Microbial Biotechnology
T1  - Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production
IS  - 3
SP  - e14445
VL  - 17
DO  - 10.1111/1751-7915.14445
ER  - 

@article{
author = "Pantelić, Brana and Siaperas, Romanos and Budin, Clémence and de Boer, Tjalf and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "Global plastic waste accumulation has become omnipresent in public discourse and the focus of scientific research. Ranking as the sixth most produced polymer globally, polyurethanes (PU) significantly contribute to plastic waste and environmental pollution due to the toxicity of their building blocks, such as diisocyanates. In this study, the effects of PU on soil microbial communities over 18 months were monitored revealing that it had marginal effects on microbial diversity. However, Streptomyces sp. PU10, isolated from this PU-contaminated soil, proved exceptional in the degradation of a soluble polyester-PU (Impranil) across a range of temperatures with over 96% degradation of 10 g/L in 48 h. Proteins involved in PU degradation and metabolic changes occurring in this strain with Impranil as the sole carbon source were further investigated employing quantitative proteomics. The proposed degradation mechanism implicated the action of three enzymes: a polyester-degrading esterase, a urethane bond-degrading amidase and an oxidoreductase. Furthermore, proteome data revealed that PU degradation intermediates were incorporated into Streptomyces sp. PU10 metabolism via the fatty acid degradation pathway and subsequently channelled to polyketide biosynthesis. Most notably, the production of the tri-pyrrole undecylprodigiosin was confirmed paving the way for establishing PU upcycling strategies to bioactive metabolites using Streptomyces strains.",
publisher = "Wiley",
journal = "Microbial Biotechnology, Microbial Biotechnology",
title = "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production",
number = "3",
pages = "e14445",
volume = "17",
doi = "10.1111/1751-7915.14445"
}

Pantelić, B., Siaperas, R., Budin, C., de Boer, T., Topakas, E.,& Nikodinović-Runić, J.. (2024). Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology
Wiley., 17(3), e14445.
https://doi.org/10.1111/1751-7915.14445

Pantelić B, Siaperas R, Budin C, de Boer T, Topakas E, Nikodinović-Runić J. Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production. in Microbial Biotechnology. 2024;17(3):e14445.
doi:10.1111/1751-7915.14445 .

Pantelić, Brana, Siaperas, Romanos, Budin, Clémence, de Boer, Tjalf, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "Proteomic examination of polyester-polyurethane degradation by Streptomyces sp. PU10: Diverting polyurethane intermediates to secondary metabolite production" in Microbial Biotechnology, 17, no. 3 (2024):e14445,
https://doi.org/10.1111/1751-7915.14445 . .

TY  - JOUR
AU  - Babić Radić, Marija
AU  - Vukomanović, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Tomić, Simonida
PY  - 2024
UR  - https://www.mdpi.com/1999-4923/16/3/372
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2330
AB  - This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO2) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO2 on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (Caenorhabditis elegans) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.
PB  - MDPI
T2  - Pharmaceutics
T2  - Pharmaceutics
T1  - Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform
IS  - 3
SP  - 372
VL  - 16
DO  - 10.3390/pharmaceutics16030372
ER  - 

@article{
author = "Babić Radić, Marija and Vukomanović, Marija and Nikodinović-Runić, Jasmina and Tomić, Simonida",
year = "2024",
abstract = "This study proposes synthesis and evaluation of gelatin-/alginate-based hydrogel scaffolds reinforced with titanium dioxide (TiO2) nanoparticles which, through their combination with allantoin, quercetin, and caffeic acid, provide multi-target therapy directed on all phases of the wound healing process. These scaffolds provide the simultaneous release of bioactive agents and concurrently support cell/tissue repair through the replicated structure of a native extracellular matrix. The hydrogel scaffolds were synthesized via a crosslinking reaction using EDC as a crosslinker for gelatin. Synthesized hydrogel scaffolds and the effect of TiO2 on their properties were characterized by structural, mechanical, morphological, and swelling properties, and the porosity, wettability, adhesion to skin tissue, and simultaneous release features. The biocompatibility of the scaffolds was tested in vitro on fibroblasts (MRC5 cells) and in vivo (Caenorhabditis elegans) in a survival probe. The scaffolds revealed porous interconnected morphology, porosity of 88.33 to 96.76%, elastic modulus of 1.53 to 4.29 MPa, full hydrophilicity, favorable skin adhesivity, and biocompatibility. The simultaneous release was investigated in vitro indicating dependence on the scaffold’s composition and type of bioactive agents. The novel scaffolds designed as multi-target therapy have significant promise for improved wound healing in a beneficial and non-invasive manner.",
publisher = "MDPI",
journal = "Pharmaceutics, Pharmaceutics",
title = "Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform",
number = "3",
pages = "372",
volume = "16",
doi = "10.3390/pharmaceutics16030372"
}

Babić Radić, M., Vukomanović, M., Nikodinović-Runić, J.,& Tomić, S.. (2024). Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform. in Pharmaceutics
MDPI., 16(3), 372.
https://doi.org/10.3390/pharmaceutics16030372

Babić Radić M, Vukomanović M, Nikodinović-Runić J, Tomić S. Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform. in Pharmaceutics. 2024;16(3):372.
doi:10.3390/pharmaceutics16030372 .

Babić Radić, Marija, Vukomanović, Marija, Nikodinović-Runić, Jasmina, Tomić, Simonida, "Gelatin-/Alginate-Based Hydrogel Scaffolds Reinforced with TiO2 Nanoparticles for Simultaneous Release of Allantoin, Caffeic Acid, and Quercetin as Multi-Target Wound Therapy Platform" in Pharmaceutics, 16, no. 3 (2024):372,
https://doi.org/10.3390/pharmaceutics16030372 . .

TY  - JOUR
AU  - Araujo, Jeovan A.
AU  - Taxeidis, George
AU  - Pereira, Everton H.
AU  - Azeem, Muhammad
AU  - Pantelić, Brana
AU  - Jeremić, Sanja
AU  - Ponjavić, Marijana
AU  - Chen, Yuanyuan
AU  - Mojicević, Marija
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
AU  - Brennan Fournet, Margaret
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0959652624004724
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2315
AB  - Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.
PB  - Elsevier
T2  - Journal of Cleaner Production
T1  - Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose
SP  - 141025
DO  - 10.1016/j.jclepro.2024.141025
ER  - 

@article{
author = "Araujo, Jeovan A. and Taxeidis, George and Pereira, Everton H. and Azeem, Muhammad and Pantelić, Brana and Jeremić, Sanja and Ponjavić, Marijana and Chen, Yuanyuan and Mojicević, Marija and Nikodinović-Runić, Jasmina and Topakas, Evangelos and Brennan Fournet, Margaret",
year = "2024",
abstract = "Ubiquitous post-consumer plastic waste is often physically mixed combining recalcitrant petroleum-based plastics with bioplastics, forming (petro-bio)plastic streams. Finding appropriate end-of-life (EoL) strategies for mixed (petro-bio)plastic waste is highly pertinent in achieving environmental protection, sustainability for plastic value chain industries including recyclers and government policy makers worldwide. The presence of bioplastic mixed in with polyethylene terephthalate (PET) or other petroleum-based plastic streams poses a substantial drawback to mechanical recycling and strongly impedes the development of sustainable EoL routes. Here, we present a model system for the sustainable management of mixed (petro-bio)plastic waste, demonstrating a biotechnological route through synergy-promoted enzymatic degradation of PET–representing petrochemical polyester plastic–mixed with thermoplastic starch (TPS)–as a model bioplastic. Leaf-branch compost cutinase (LCCICCG) and commercial amylase (AMY) deliver effective depolymerization of this mixed (petro-bio)plastic material, with subsequent bio-upcycling of the mixed waste stream into bacterial nanocellulose (BNC) by Komagataeibacter medellinensis. Compared to LCCICCG and AMY, the LCCICCG/AMY combined treatment synergistically produced a 2.6- and 4.4-fold increase in enzymatic decomposition at 70 °C in four days, respectively, yielding sugars and terephthalic acid (TPA) as the main depolymerization building blocks. Bio-upcycling of post-enzymatic degradation hydrolysates resulted in a high BNC yield of 3 g L−1 after 10 days. This work paves the way for sustainable management routes for challenging mixed recalcitrant plastic and bioplastic waste and prepares opportunities for its participation in the circular production of sustainable eco-polymers.",
publisher = "Elsevier",
journal = "Journal of Cleaner Production",
title = "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose",
pages = "141025",
doi = "10.1016/j.jclepro.2024.141025"
}

Araujo, J. A., Taxeidis, G., Pereira, E. H., Azeem, M., Pantelić, B., Jeremić, S., Ponjavić, M., Chen, Y., Mojicević, M., Nikodinović-Runić, J., Topakas, E.,& Brennan Fournet, M.. (2024). Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production
Elsevier., 141025.
https://doi.org/10.1016/j.jclepro.2024.141025

Araujo JA, Taxeidis G, Pereira EH, Azeem M, Pantelić B, Jeremić S, Ponjavić M, Chen Y, Mojicević M, Nikodinović-Runić J, Topakas E, Brennan Fournet M. Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose. in Journal of Cleaner Production. 2024;:141025.
doi:10.1016/j.jclepro.2024.141025 .

Araujo, Jeovan A., Taxeidis, George, Pereira, Everton H., Azeem, Muhammad, Pantelić, Brana, Jeremić, Sanja, Ponjavić, Marijana, Chen, Yuanyuan, Mojicević, Marija, Nikodinović-Runić, Jasmina, Topakas, Evangelos, Brennan Fournet, Margaret, "Biotechnological model for ubiquitous mixed petroleum- and bio-based plastics degradation and upcycling into bacterial nanocellulose" in Journal of Cleaner Production (2024):141025,
https://doi.org/10.1016/j.jclepro.2024.141025 . .

TY  - JOUR
AU  - Stanković, Mia
AU  - Kljun, Jakob
AU  - Stevanović, Nevena Lj.
AU  - Lazić, Jelena
AU  - Škaro Bogojević, Sanja
AU  - Vojnović, Sandra
AU  - Zlatar, Matija
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Đuran, Miloš
AU  - Glišić, Biljana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2303
AB  - Inspired by the emergence of resistance to currently available antifungal therapy and by the great potential of metal complexes for the treatment of various diseases, we synthesized three new silver(I) complexes containing clinically used antifungal azoles as ligands, [Ag(ecz)2]SbF6 (1, ecz is econazole), {[Ag(vcz)2]SbF6}n (2, vcz is voriconazole), and [Ag(ctz)2]SbF6 (3, ctz is clotrimazole), and investigated their antimicrobial properties. The synthesized complexes were characterized by mass spectrometry, IR, UV-vis and 1H NMR spectroscopy, cyclic voltammetry, and single-crystal X-ray diffraction analysis. In the mononuclear complexes 1 and 3 with ecz and ctz, respectively, the silver(I) ion has the expected linear geometry, in which the azoles are monodentately coordinated to this metal center through the N3 imidazole nitrogen atom. In contrast, the vcz-containing complex 2 has a polymeric structure in the solid state in which the silver(I) ions are coordinated by four nitrogen atoms in a distorted tetrahedral geometry. DFT calculations were done to predict the most favorable structures of the studied complexes in DMSO solution. All the studied silver(I) complexes have shown excellent antifungal and good to moderate antibacterial activities with minimal inhibitory concentration (MIC) values in the ranges of 0.01–27.1 and 2.61–47.9 μM on the selected panel of fungi and bacteria, respectively. Importantly, the complexes 1–3 have exhibited a significantly improved antifungal activity compared to the free azoles, with the most pronounced effect observed in the case of complex 2 compared to the parent vcz against Candida glabrata with an increase of activity by five orders of magnitude. Moreover, the silver(I)-azole complexes 2 and 3 significantly inhibited the formation of C. albicans hyphae and biofilms at the subinhibitory concentration of 50% MIC. To investigate the impact of the complex 3 more thoroughly on Candida pathogenesis, its effect on the adherence of C. albicans to A549 cells (human adenocarcinoma alveolar basal epithelial cells), as an initial step of the invasion of host cells, was studied.
PB  - Royal Society of Chemistry (RSC)
T2  - Dalton Transactions
T1  - Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion
DO  - 10.1039/D3DT03010E
ER  - 

@article{
author = "Stanković, Mia and Kljun, Jakob and Stevanović, Nevena Lj. and Lazić, Jelena and Škaro Bogojević, Sanja and Vojnović, Sandra and Zlatar, Matija and Nikodinović-Runić, Jasmina and Turel, Iztok and Đuran, Miloš and Glišić, Biljana",
year = "2024",
abstract = "Inspired by the emergence of resistance to currently available antifungal therapy and by the great potential of metal complexes for the treatment of various diseases, we synthesized three new silver(I) complexes containing clinically used antifungal azoles as ligands, [Ag(ecz)2]SbF6 (1, ecz is econazole), {[Ag(vcz)2]SbF6}n (2, vcz is voriconazole), and [Ag(ctz)2]SbF6 (3, ctz is clotrimazole), and investigated their antimicrobial properties. The synthesized complexes were characterized by mass spectrometry, IR, UV-vis and 1H NMR spectroscopy, cyclic voltammetry, and single-crystal X-ray diffraction analysis. In the mononuclear complexes 1 and 3 with ecz and ctz, respectively, the silver(I) ion has the expected linear geometry, in which the azoles are monodentately coordinated to this metal center through the N3 imidazole nitrogen atom. In contrast, the vcz-containing complex 2 has a polymeric structure in the solid state in which the silver(I) ions are coordinated by four nitrogen atoms in a distorted tetrahedral geometry. DFT calculations were done to predict the most favorable structures of the studied complexes in DMSO solution. All the studied silver(I) complexes have shown excellent antifungal and good to moderate antibacterial activities with minimal inhibitory concentration (MIC) values in the ranges of 0.01–27.1 and 2.61–47.9 μM on the selected panel of fungi and bacteria, respectively. Importantly, the complexes 1–3 have exhibited a significantly improved antifungal activity compared to the free azoles, with the most pronounced effect observed in the case of complex 2 compared to the parent vcz against Candida glabrata with an increase of activity by five orders of magnitude. Moreover, the silver(I)-azole complexes 2 and 3 significantly inhibited the formation of C. albicans hyphae and biofilms at the subinhibitory concentration of 50% MIC. To investigate the impact of the complex 3 more thoroughly on Candida pathogenesis, its effect on the adherence of C. albicans to A549 cells (human adenocarcinoma alveolar basal epithelial cells), as an initial step of the invasion of host cells, was studied.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Dalton Transactions",
title = "Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion",
doi = "10.1039/D3DT03010E"
}

Stanković, M., Kljun, J., Stevanović, N. Lj., Lazić, J., Škaro Bogojević, S., Vojnović, S., Zlatar, M., Nikodinović-Runić, J., Turel, I., Đuran, M.,& Glišić, B.. (2024). Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion. in Dalton Transactions
Royal Society of Chemistry (RSC)..
https://doi.org/10.1039/D3DT03010E

Stanković M, Kljun J, Stevanović NL, Lazić J, Škaro Bogojević S, Vojnović S, Zlatar M, Nikodinović-Runić J, Turel I, Đuran M, Glišić B. Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion. in Dalton Transactions. 2024;.
doi:10.1039/D3DT03010E .

Stanković, Mia, Kljun, Jakob, Stevanović, Nevena Lj., Lazić, Jelena, Škaro Bogojević, Sanja, Vojnović, Sandra, Zlatar, Matija, Nikodinović-Runić, Jasmina, Turel, Iztok, Đuran, Miloš, Glišić, Biljana, "Silver(I) complexes containing antifungal azoles: significant improvement of the anti-Candida potential of the azole drug after its coordination to the silver(I) ion" in Dalton Transactions (2024),
https://doi.org/10.1039/D3DT03010E . .

TY  - JOUR
AU  - Vojnović, Sandra
AU  - Aleksić, Ivana
AU  - Ilić-Tomić, Tatjana
AU  - Stevanović, Milena
AU  - Nikodinović-Runić, Jasmina
PY  - 2024
UR  - https://doi.org/10.1007/s00253-023-12900-x
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2314
AB  - The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.
PB  - Springer Nature
T2  - Applied Microbiology and Biotechnology
T1  - Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes
IS  - 1
SP  - 185
VL  - 108
DO  - 10.1007/s00253-023-12900-x
ER  - 

@article{
author = "Vojnović, Sandra and Aleksić, Ivana and Ilić-Tomić, Tatjana and Stevanović, Milena and Nikodinović-Runić, Jasmina",
year = "2024",
abstract = "The application of enzymes is expanding across diverse industries due to their nontoxic and biodegradable characteristics. Another advantage is their cost-effectiveness, reflected in reduced processing time, water, and energy consumption. Although Gram-positive bacteria, Bacillus, and Streptomyces spp. are successfully used for production of industrially relevant enzymes, they still lag far behind Escherichia coli as hosts for recombinant protein production. Generally, proteins secreted by Bacillus and Streptomyces hosts are released into the culture medium; their native conformation is preserved and easier recovery process enabled. Given the resilience of both hosts in harsh environmental conditions and their spore-forming capability, a deeper understanding and broader use of Bacillus and Streptomyces as expression hosts could significantly enhance the robustness of industrial bioprocesses. This mini-review aims to compare two expression hosts, emphasizing their specific advantages in industrial surroundings such are chemical, detergent, textile, food, animal feed, leather, and paper industries. The homologous sources, heterologous hosts, and molecular tools used for the production of recombinant proteins in these hosts are discussed. The potential to use both hosts as biocatalysts is also evaluated. Undoubtedly, Bacillus and Streptomyces spp. as production hosts possess the potential to take on a more substantial role, providing superior (bio-based) process robustness and flexibility.",
publisher = "Springer Nature",
journal = "Applied Microbiology and Biotechnology",
title = "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes",
number = "1",
pages = "185",
volume = "108",
doi = "10.1007/s00253-023-12900-x"
}

Vojnović, S., Aleksić, I., Ilić-Tomić, T., Stevanović, M.,& Nikodinović-Runić, J.. (2024). Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology
Springer Nature., 108(1), 185.
https://doi.org/10.1007/s00253-023-12900-x

Vojnović S, Aleksić I, Ilić-Tomić T, Stevanović M, Nikodinović-Runić J. Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes. in Applied Microbiology and Biotechnology. 2024;108(1):185.
doi:10.1007/s00253-023-12900-x .

Vojnović, Sandra, Aleksić, Ivana, Ilić-Tomić, Tatjana, Stevanović, Milena, Nikodinović-Runić, Jasmina, "Bacillus and Streptomyces spp. as hosts for production of industrially relevant enzymes" in Applied Microbiology and Biotechnology, 108, no. 1 (2024):185,
https://doi.org/10.1007/s00253-023-12900-x . .

TY  - CONF
AU  - Ponjavić, Marijana
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Đurašković, Nikola
AU  - Tica, Nedeljko
AU  - Živković, Irena
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2334
AB  - In this paper, the biodegradability of three SferiCorn™
biocomposites was investigated. Corn stover prepared through eco-friendly
washing and grinding to short fibers was used as reinforcement. Three different
biopolymers, corn starch, alginate and poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) (PHBV), were used as matrices. The biodegradability of
prepared composites was investigated in a simulated soil burial test and the
samples were characterized in terms of weight loss and morphological changes. It
was shown that biodegradability can be tuned by biopolymers used as matrices.
Changes in the surface morphology after biodegradation of tested samples were
confirmed using scanning electron microscopy (SEM).
PB  - Čačak : Faculty of Agronomy
C3  - 2nd International symposium on biotechnology
T1  - Biodegradability assessment of corn stover reinforced composite materials with different matrix
EP  - 261
SP  - 225
DO  - 10.46793/SBT29
ER  - 

@conference{
author = "Ponjavić, Marijana and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Đurašković, Nikola and Tica, Nedeljko and Živković, Irena",
year = "2024",
abstract = "In this paper, the biodegradability of three SferiCorn™
biocomposites was investigated. Corn stover prepared through eco-friendly
washing and grinding to short fibers was used as reinforcement. Three different
biopolymers, corn starch, alginate and poly(3-hydroxybutyrate-co-3-
hydroxyvalerate) (PHBV), were used as matrices. The biodegradability of
prepared composites was investigated in a simulated soil burial test and the
samples were characterized in terms of weight loss and morphological changes. It
was shown that biodegradability can be tuned by biopolymers used as matrices.
Changes in the surface morphology after biodegradation of tested samples were
confirmed using scanning electron microscopy (SEM).",
publisher = "Čačak : Faculty of Agronomy",
journal = "2nd International symposium on biotechnology",
title = "Biodegradability assessment of corn stover reinforced composite materials with different matrix",
pages = "261-225",
doi = "10.46793/SBT29"
}

Ponjavić, M., Milovanović, J., Nikodinović-Runić, J., Đurašković, N., Tica, N.,& Živković, I.. (2024). Biodegradability assessment of corn stover reinforced composite materials with different matrix. in 2nd International symposium on biotechnology
Čačak : Faculty of Agronomy., 225-261.
https://doi.org/10.46793/SBT29

Ponjavić M, Milovanović J, Nikodinović-Runić J, Đurašković N, Tica N, Živković I. Biodegradability assessment of corn stover reinforced composite materials with different matrix. in 2nd International symposium on biotechnology. 2024;:225-261.
doi:10.46793/SBT29 .

Ponjavić, Marijana, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Đurašković, Nikola, Tica, Nedeljko, Živković, Irena, "Biodegradability assessment of corn stover reinforced composite materials with different matrix" in 2nd International symposium on biotechnology (2024):225-261,
https://doi.org/10.46793/SBT29 . .

TY  - JOUR
AU  - Škaro Bogojević, Sanja
AU  - Perminova, D
AU  - Jaksic, J
AU  - Milcic, M
AU  - Medakovic, V
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, V
PY  - 2024
UR  - https://www.sciencedirect.com/science/article/pii/S0022286023023797
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2274
AB  - Poly(ethylene terephthalate) (PET) is widely used material in the healthcare due to its mechanical properties including resistance to chemicals and abrasion. However, it is susceptible to bacterial attachment and contamination. This study addresses some newly designed model compounds of PET with antimicrobial properties that could potentially be incorporated into PET materials. All compounds were synthesized for the first time by labeling an integral part of PET with chromophores in the form of esters of cinnamic and ferulic acids. After complete structural characterization, the effect of new compounds on microbial growth and communication (quorum sensing, QS) was analyzed and further investigated using molecular docking. The obtained results indicate that the introduction of chromophores that have one part of cinnamic acid enriched with a methoxy functional group in them acts as QS modulators. Moreover, compounds exhibited dose-dependent selectivity toward QS signaling pathways and the highest tested concentration of compounds showed Pseudomonas Quinolone Signal (PQS) inhibitory activity suggesting that these compounds have a potential effect on pyocyanin production. Docking studies demonstrated that compounds hold binding power to all four QS protein targets (LuxP, periplasmatic protein that binds AI-2 inducer and forms a complex able to transduce the autoinducer signal, RhIR protein that is a key QS transcriptional regulator that activates the genes involved in the synthesis of rhamnolipids and pyocyanin, AbaI protein that has a role in QS signal transduction, and LasR protein which is a key QS transcriptional regulator that activates transcription of genes coding for some virulence-associated traits) while the highest binding strength is observed with compounds 2 and 6 containing single cinnamic acid fragment, suggesting their further biomedical application.
T2  - Journal of Molecular Structure
T1  - Novel cinnamic acid-based PET derivatives as quorum sensing modulators
SP  - 137291
VL  - 1300
DO  - 10.1016/j.molstruc.2023.137291
ER  - 

@article{
author = "Škaro Bogojević, Sanja and Perminova, D and Jaksic, J and Milcic, M and Medakovic, V and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Maslak, V",
year = "2024",
abstract = "Poly(ethylene terephthalate) (PET) is widely used material in the healthcare due to its mechanical properties including resistance to chemicals and abrasion. However, it is susceptible to bacterial attachment and contamination. This study addresses some newly designed model compounds of PET with antimicrobial properties that could potentially be incorporated into PET materials. All compounds were synthesized for the first time by labeling an integral part of PET with chromophores in the form of esters of cinnamic and ferulic acids. After complete structural characterization, the effect of new compounds on microbial growth and communication (quorum sensing, QS) was analyzed and further investigated using molecular docking. The obtained results indicate that the introduction of chromophores that have one part of cinnamic acid enriched with a methoxy functional group in them acts as QS modulators. Moreover, compounds exhibited dose-dependent selectivity toward QS signaling pathways and the highest tested concentration of compounds showed Pseudomonas Quinolone Signal (PQS) inhibitory activity suggesting that these compounds have a potential effect on pyocyanin production. Docking studies demonstrated that compounds hold binding power to all four QS protein targets (LuxP, periplasmatic protein that binds AI-2 inducer and forms a complex able to transduce the autoinducer signal, RhIR protein that is a key QS transcriptional regulator that activates the genes involved in the synthesis of rhamnolipids and pyocyanin, AbaI protein that has a role in QS signal transduction, and LasR protein which is a key QS transcriptional regulator that activates transcription of genes coding for some virulence-associated traits) while the highest binding strength is observed with compounds 2 and 6 containing single cinnamic acid fragment, suggesting their further biomedical application.",
journal = "Journal of Molecular Structure",
title = "Novel cinnamic acid-based PET derivatives as quorum sensing modulators",
pages = "137291",
volume = "1300",
doi = "10.1016/j.molstruc.2023.137291"
}

Škaro Bogojević, S., Perminova, D., Jaksic, J., Milcic, M., Medakovic, V., Milovanović, J., Nikodinović-Runić, J.,& Maslak, V.. (2024). Novel cinnamic acid-based PET derivatives as quorum sensing modulators. in Journal of Molecular Structure, 1300, 137291.
https://doi.org/10.1016/j.molstruc.2023.137291

Škaro Bogojević S, Perminova D, Jaksic J, Milcic M, Medakovic V, Milovanović J, Nikodinović-Runić J, Maslak V. Novel cinnamic acid-based PET derivatives as quorum sensing modulators. in Journal of Molecular Structure. 2024;1300:137291.
doi:10.1016/j.molstruc.2023.137291 .

Škaro Bogojević, Sanja, Perminova, D, Jaksic, J, Milcic, M, Medakovic, V, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Maslak, V, "Novel cinnamic acid-based PET derivatives as quorum sensing modulators" in Journal of Molecular Structure, 1300 (2024):137291,
https://doi.org/10.1016/j.molstruc.2023.137291 . .

TY  - JOUR
AU  - Taxeidis, George
AU  - Nikolaivits, Efstratios
AU  - Siaperas, Romanos
AU  - Gkountela, Christina
AU  - Vouyiouka, Stamatina
AU  - Pantelić, Brana
AU  - Nikodinović-Runić, Jasmina
AU  - Topakas, Evangelos
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0269749123004621
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1797
AB  - The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.
T2  - Environmental Pollution
T1  - Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes
SP  - 121460
VL  - 325
DO  - 10.1016/j.envpol.2023.121460
ER  - 

@article{
author = "Taxeidis, George and Nikolaivits, Efstratios and Siaperas, Romanos and Gkountela, Christina and Vouyiouka, Stamatina and Pantelić, Brana and Nikodinović-Runić, Jasmina and Topakas, Evangelos",
year = "2023",
abstract = "The uncontrollable disposal of plastic waste has raised the concern of the scientific community, which tries to face this environmental burden by discovering and applying new techniques. Regarding the biotechnology field, several important microorganisms possessing the necessary enzymatic arsenal to utilize recalcitrant synthetic polymers as an energy source have been discovered. In the present study, we screened various fungi for their ability to degrade intact polymers, such as ether-based polyurethane (PU) and low-density polyethylene (LDPE). For this, ImpranIil® DLN-SD and a mixture of long-chain alkanes were used as sole carbon sources, indicating not only the most promising strains in agar plate screening but also inducing the secretion of depolymerizing enzymatic activities, useful for polymer degradation. The agar plate screening revealed three fungal strains belonging to Fusarium and Aspergillus genera, whose secretome was further studied for its ability to degrade the aforementioned non-treated polymers. Specifically for ether-based PU, the secretome of a Fusarium species reduced the sample mass and the average molecular weight of the polymer by 24.5 and 20.4%, respectively, while the secretome of an Aspergillus species caused changes in the molecular structure of LDPE, as evidenced by FTIR. The proteomics analysis revealed that the enzymatic activities induced in presence of Impranil® DLN-SD can be associated with urethane bond cleavage, a fact which was also supported by the observed degradation of the ether-based PU. Although, the mechanism of LDPE degradation was not completely elucidated, the presence of oxidative enzymes could be the main factor contributing to polymer modification.",
journal = "Environmental Pollution",
title = "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes",
pages = "121460",
volume = "325",
doi = "10.1016/j.envpol.2023.121460"
}

Taxeidis, G., Nikolaivits, E., Siaperas, R., Gkountela, C., Vouyiouka, S., Pantelić, B., Nikodinović-Runić, J.,& Topakas, E.. (2023). Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution, 325, 121460.
https://doi.org/10.1016/j.envpol.2023.121460

Taxeidis G, Nikolaivits E, Siaperas R, Gkountela C, Vouyiouka S, Pantelić B, Nikodinović-Runić J, Topakas E. Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes. in Environmental Pollution. 2023;325:121460.
doi:10.1016/j.envpol.2023.121460 .

Taxeidis, George, Nikolaivits, Efstratios, Siaperas, Romanos, Gkountela, Christina, Vouyiouka, Stamatina, Pantelić, Brana, Nikodinović-Runić, Jasmina, Topakas, Evangelos, "Triggering and identifying the polyurethane and polyethylene-degrading machinery of filamentous fungi secretomes" in Environmental Pollution, 325 (2023):121460,
https://doi.org/10.1016/j.envpol.2023.121460 . .

TY  - CONF
AU  - Pešović, Tijana
AU  - Kovačević, Aleksandar
AU  - Nikodinović-Runić, Jasmina
AU  - Ponjavić, Marijana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2207
AB  - In the fight against oil and it's derivatives pollution, adsorbents play a crucial role in
efficient removal of these harmful substances from the environment. Jute, a natural plant
fiber, is gaining increasing attention as a potential adsorbent for oil due to high porosity,
good physical and chemical characteristics, biodegradability, and sustainability [1]. The
aim of this study was to investigate the biodegradable properties of jute used as an
absorbent for oil in a liquid medium and model compost. Materials and methods: The
study monitored weight loss in oil-contaminated jute (NWSO) and compared it with
control jute (NWS). Morphological changes in jute fibers were observed with optical
microscopy and SEM analysis. Gas chromatography (GC-MS) was used for the
detection of hydrocarbons in degraded jute. Enzymatic activity changes in compost soil
were tracked. Bacterial strain isolation was done to assess growth on pure and oilcontaminated
jute, with subsequent taxonomic identification. Results: NWSO samples
were degraded more efficiently compared to clean jute with a weight reduction of 20%
in NWSO compared to 5% in NWS. Microscopic and SEM analyses confirmed
morphological changes in jute fibers after degradation. A decrease in hydrocarbon
concentration after degradation was shown. Enzymatic activity tests provided additional
insights into the composting process. The study also identified diverse bacterial strains
capable of oil degradation, primarily belonging to Bacillus and Microbacterium genera.
Conclusion: The study demonstrates the superior biodegradation of NWSO compared
to NWS. The promising role of jute in sustainable bioremediation strategies leading to
reduced harm from oil pollution has been demonstrated.
PB  - Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia
T1  - Biological degradation of recycled jute used as an adsorbent for crude oil
EP  - 95
SP  - 95
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2207
ER  - 

@conference{
author = "Pešović, Tijana and Kovačević, Aleksandar and Nikodinović-Runić, Jasmina and Ponjavić, Marijana",
year = "2023",
abstract = "In the fight against oil and it's derivatives pollution, adsorbents play a crucial role in
efficient removal of these harmful substances from the environment. Jute, a natural plant
fiber, is gaining increasing attention as a potential adsorbent for oil due to high porosity,
good physical and chemical characteristics, biodegradability, and sustainability [1]. The
aim of this study was to investigate the biodegradable properties of jute used as an
absorbent for oil in a liquid medium and model compost. Materials and methods: The
study monitored weight loss in oil-contaminated jute (NWSO) and compared it with
control jute (NWS). Morphological changes in jute fibers were observed with optical
microscopy and SEM analysis. Gas chromatography (GC-MS) was used for the
detection of hydrocarbons in degraded jute. Enzymatic activity changes in compost soil
were tracked. Bacterial strain isolation was done to assess growth on pure and oilcontaminated
jute, with subsequent taxonomic identification. Results: NWSO samples
were degraded more efficiently compared to clean jute with a weight reduction of 20%
in NWSO compared to 5% in NWS. Microscopic and SEM analyses confirmed
morphological changes in jute fibers after degradation. A decrease in hydrocarbon
concentration after degradation was shown. Enzymatic activity tests provided additional
insights into the composting process. The study also identified diverse bacterial strains
capable of oil degradation, primarily belonging to Bacillus and Microbacterium genera.
Conclusion: The study demonstrates the superior biodegradation of NWSO compared
to NWS. The promising role of jute in sustainable bioremediation strategies leading to
reduced harm from oil pollution has been demonstrated.",
publisher = "Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia",
title = "Biological degradation of recycled jute used as an adsorbent for crude oil",
pages = "95-95",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2207"
}

Pešović, T., Kovačević, A., Nikodinović-Runić, J.,& Ponjavić, M.. (2023). Biological degradation of recycled jute used as an adsorbent for crude oil. in 9th Conference of Young Chemists of Serbia
Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club., 95-95.
https://hdl.handle.net/21.15107/rcub_imagine_2207

Pešović T, Kovačević A, Nikodinović-Runić J, Ponjavić M. Biological degradation of recycled jute used as an adsorbent for crude oil. in 9th Conference of Young Chemists of Serbia. 2023;:95-95.
https://hdl.handle.net/21.15107/rcub_imagine_2207 .

Pešović, Tijana, Kovačević, Aleksandar, Nikodinović-Runić, Jasmina, Ponjavić, Marijana, "Biological degradation of recycled jute used as an adsorbent for crude oil" in 9th Conference of Young Chemists of Serbia (2023):95-95,
https://hdl.handle.net/21.15107/rcub_imagine_2207 .

TY  - CONF
AU  - Sourkouni, G.
AU  - Kalogirou, Ch.
AU  - Papadimitriou, N.
AU  - Nenadović, Marija
AU  - Ponjavić, Marijana
AU  - Argirusis, N.
AU  - Pandis, P.
AU  - Rajasekaran, D.
AU  - Padamati, R.
AU  - Ferraro, A.
AU  - Nikodinović-Runić, Jasmina
AU  - Argirusis, Chr.
PY  - 2023
UR  - http://chania2023.uest.gr/poster.html
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2024
AB  - Plastics are artificial synthetic organic polymers that have been used in every area of daily life. However, because of their slow degradation rate, their use
is contentious. The treatment of the surface of the sample is considered necessary as enzymatic or bacterial attach is not possible, if the plastic surface
environment is not ideal. The main topic of this work is the investigation of the effect of atmospheric dielectric barrier discharge (DBD) plasma on the near
surface structure of polylactic acid (PLA) samples, which, in turn, can promote the adhesion of enzymes or bacteria for further biodegradation. In general,
plasma processes can already be considered as inherently environmental technologies.
Plasma processes enable resource saving through high energy utilization efficiency and thus, are environ-mentally friendly technologies. Atmospheric
pressure discharges (APDs) are useful because of their specific advantages over low-pressure ones. They do not need expensive vacuum equipment, and
generate nonthermal plasmas, which are more suitable for assembly line processes. Hence, this category of discharges has significant industrial
applications. The use of a dielectric barrier in the discharge gap helps prevent spark formation. DBDs exhibit two major discharge modes: filamentary and
glow (homogeneous). The glow discharge mode has obvious advantages over the filamentary one for applications such as treatment of surfaces and
deposition of thin films. Glow mode discharges with average power densities comparable to those of filamentary discharges are of enormous interest for
applications in which reliable control is required.
Here we will present the increased adhesion of bacteria strains on DBD plasma treated PLA foils which can lead to a better degradation of the PLA. X-ray
photoelectron spectroscopy (XPS) measurements of the foils prior to and after the treatment proved the changes on the polymer surface. A short
discussion of the possibilities the treatment opens is given.
C3  - 10th International Conference on Sustainable Solid Waste Management Chania
T1  - Plasma assisted bio-degradation  of poly-lactic acid (PLA)
VL  - 10
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2024
ER  - 

@conference{
author = "Sourkouni, G. and Kalogirou, Ch. and Papadimitriou, N. and Nenadović, Marija and Ponjavić, Marijana and Argirusis, N. and Pandis, P. and Rajasekaran, D. and Padamati, R. and Ferraro, A. and Nikodinović-Runić, Jasmina and Argirusis, Chr.",
year = "2023",
abstract = "Plastics are artificial synthetic organic polymers that have been used in every area of daily life. However, because of their slow degradation rate, their use
is contentious. The treatment of the surface of the sample is considered necessary as enzymatic or bacterial attach is not possible, if the plastic surface
environment is not ideal. The main topic of this work is the investigation of the effect of atmospheric dielectric barrier discharge (DBD) plasma on the near
surface structure of polylactic acid (PLA) samples, which, in turn, can promote the adhesion of enzymes or bacteria for further biodegradation. In general,
plasma processes can already be considered as inherently environmental technologies.
Plasma processes enable resource saving through high energy utilization efficiency and thus, are environ-mentally friendly technologies. Atmospheric
pressure discharges (APDs) are useful because of their specific advantages over low-pressure ones. They do not need expensive vacuum equipment, and
generate nonthermal plasmas, which are more suitable for assembly line processes. Hence, this category of discharges has significant industrial
applications. The use of a dielectric barrier in the discharge gap helps prevent spark formation. DBDs exhibit two major discharge modes: filamentary and
glow (homogeneous). The glow discharge mode has obvious advantages over the filamentary one for applications such as treatment of surfaces and
deposition of thin films. Glow mode discharges with average power densities comparable to those of filamentary discharges are of enormous interest for
applications in which reliable control is required.
Here we will present the increased adhesion of bacteria strains on DBD plasma treated PLA foils which can lead to a better degradation of the PLA. X-ray
photoelectron spectroscopy (XPS) measurements of the foils prior to and after the treatment proved the changes on the polymer surface. A short
discussion of the possibilities the treatment opens is given.",
journal = "10th International Conference on Sustainable Solid Waste Management Chania",
title = "Plasma assisted bio-degradation  of poly-lactic acid (PLA)",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2024"
}

Sourkouni, G., Kalogirou, Ch., Papadimitriou, N., Nenadović, M., Ponjavić, M., Argirusis, N., Pandis, P., Rajasekaran, D., Padamati, R., Ferraro, A., Nikodinović-Runić, J.,& Argirusis, Chr.. (2023). Plasma assisted bio-degradation  of poly-lactic acid (PLA). in 10th International Conference on Sustainable Solid Waste Management Chania, 10.
https://hdl.handle.net/21.15107/rcub_imagine_2024

Sourkouni G, Kalogirou C, Papadimitriou N, Nenadović M, Ponjavić M, Argirusis N, Pandis P, Rajasekaran D, Padamati R, Ferraro A, Nikodinović-Runić J, Argirusis C. Plasma assisted bio-degradation  of poly-lactic acid (PLA). in 10th International Conference on Sustainable Solid Waste Management Chania. 2023;10.
https://hdl.handle.net/21.15107/rcub_imagine_2024 .

Sourkouni, G., Kalogirou, Ch., Papadimitriou, N., Nenadović, Marija, Ponjavić, Marijana, Argirusis, N., Pandis, P., Rajasekaran, D., Padamati, R., Ferraro, A., Nikodinović-Runić, Jasmina, Argirusis, Chr., "Plasma assisted bio-degradation  of poly-lactic acid (PLA)" in 10th International Conference on Sustainable Solid Waste Management Chania, 10 (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2024 .

TY  - JOUR
AU  - Özcan, Esma
AU  - Vagolu, Siva Krishna
AU  - Gündüz, Miyase Gözde
AU  - Stevanović, Milena
AU  - Kökbudak, Zülbiye
AU  - Tønjum, Tone
AU  - Nikodinović-Runić, Jasmina
AU  - Çetinkaya, Yasin
AU  - Doğan, Şengül Dilem
PY  - 2023
UR  - https://doi.org/10.1021/acsomega.3c03018
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2154
AB  - The discovery of new antimicrobial agents as a means of treating drug-resistant microbial pathogens is of utmost significance to overcome their immense risk to human well-being. The current investigation involves the development, synthesis, and assessment of the antimicrobial efficacy of novel quinoline derivatives incorporating a thiosemicarbazide functionality. To design the target compounds (QST1–QST14), we applied the molecular hybridization approach to link various thiosemicarbazides to the quinoline core with a sulfonyl group. Upon the synthesis and completion of structural characterization via spectroscopic techniques (1H NMR, 13C NMR, 15N NMR, IR, and HRMS), the title molecules were extensively evaluated for their potential antitubercular, antibacterial, and antifungal activities. N-(3-Chlorophenyl)-2-(quinolin-8-ylsulfonyl)hydrazine-1-carbothioamide (QST4), the most effective compound against Mycobacterium tuberculosis H37Rv, was also tested on isoniazid-resistant clinical isolates with katG and inhA promoter mutations. Based on molecular docking studies, QST4 was also likely to demonstrate its antimycobacterial activity through inhibition of the InhA enzyme. Furthermore, three derivatives (QST3, QST4, and QST10) with preferable antimicrobial and drug-like profiles were also shown to be nontoxic against human embryonic kidney (HEK) cells. All compounds were optimized by the density functional theory method using B3LYP with the 6-31+G(d,p) basis set. Structural analysis, natural bond orbital calculations of donor–acceptor interactions, molecular electrostatic potential analysis, and frontier molecular orbital analysis were carried out. Quantum chemical descriptors and charges on the atoms were determined to compare the strengths of the intramolecular hydrogen bonds formed and their stabilities. We determined that the sulfur atom forms a stronger intramolecular hydrogen bond than the nitrogen, oxygen, and fluorine atoms in these sulfonyl thiosemicarbazide derivatives.
T2  - ACS Omega
T1  - Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities
EP  - 40152
IS  - 43
SP  - 40140
VL  - 8
DO  - 10.1021/acsomega.3c03018
ER  - 

@article{
author = "Özcan, Esma and Vagolu, Siva Krishna and Gündüz, Miyase Gözde and Stevanović, Milena and Kökbudak, Zülbiye and Tønjum, Tone and Nikodinović-Runić, Jasmina and Çetinkaya, Yasin and Doğan, Şengül Dilem",
year = "2023",
abstract = "The discovery of new antimicrobial agents as a means of treating drug-resistant microbial pathogens is of utmost significance to overcome their immense risk to human well-being. The current investigation involves the development, synthesis, and assessment of the antimicrobial efficacy of novel quinoline derivatives incorporating a thiosemicarbazide functionality. To design the target compounds (QST1–QST14), we applied the molecular hybridization approach to link various thiosemicarbazides to the quinoline core with a sulfonyl group. Upon the synthesis and completion of structural characterization via spectroscopic techniques (1H NMR, 13C NMR, 15N NMR, IR, and HRMS), the title molecules were extensively evaluated for their potential antitubercular, antibacterial, and antifungal activities. N-(3-Chlorophenyl)-2-(quinolin-8-ylsulfonyl)hydrazine-1-carbothioamide (QST4), the most effective compound against Mycobacterium tuberculosis H37Rv, was also tested on isoniazid-resistant clinical isolates with katG and inhA promoter mutations. Based on molecular docking studies, QST4 was also likely to demonstrate its antimycobacterial activity through inhibition of the InhA enzyme. Furthermore, three derivatives (QST3, QST4, and QST10) with preferable antimicrobial and drug-like profiles were also shown to be nontoxic against human embryonic kidney (HEK) cells. All compounds were optimized by the density functional theory method using B3LYP with the 6-31+G(d,p) basis set. Structural analysis, natural bond orbital calculations of donor–acceptor interactions, molecular electrostatic potential analysis, and frontier molecular orbital analysis were carried out. Quantum chemical descriptors and charges on the atoms were determined to compare the strengths of the intramolecular hydrogen bonds formed and their stabilities. We determined that the sulfur atom forms a stronger intramolecular hydrogen bond than the nitrogen, oxygen, and fluorine atoms in these sulfonyl thiosemicarbazide derivatives.",
journal = "ACS Omega",
title = "Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities",
pages = "40152-40140",
number = "43",
volume = "8",
doi = "10.1021/acsomega.3c03018"
}

Özcan, E., Vagolu, S. K., Gündüz, M. G., Stevanović, M., Kökbudak, Z., Tønjum, T., Nikodinović-Runić, J., Çetinkaya, Y.,& Doğan, Ş. D.. (2023). Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities. in ACS Omega, 8(43), 40140-40152.
https://doi.org/10.1021/acsomega.3c03018

Özcan E, Vagolu SK, Gündüz MG, Stevanović M, Kökbudak Z, Tønjum T, Nikodinović-Runić J, Çetinkaya Y, Doğan ŞD. Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities. in ACS Omega. 2023;8(43):40140-40152.
doi:10.1021/acsomega.3c03018 .

Özcan, Esma, Vagolu, Siva Krishna, Gündüz, Miyase Gözde, Stevanović, Milena, Kökbudak, Zülbiye, Tønjum, Tone, Nikodinović-Runić, Jasmina, Çetinkaya, Yasin, Doğan, Şengül Dilem, "Novel Quinoline-Based Thiosemicarbazide Derivatives: Synthesis, DFT Calculations, and Investigation of Antitubercular, Antibacterial, and Antifungal Activities" in ACS Omega, 8, no. 43 (2023):40140-40152,
https://doi.org/10.1021/acsomega.3c03018 . .

TY  - JOUR
AU  - Andrejević, Tina
AU  - Aleksić, Ivana
AU  - Kljun, Jakob
AU  - Počkaj, Marta
AU  - Zlatar, Matija
AU  - Vojnović, Sandra
AU  - Nikodinović-Runić, Jasmina
AU  - Turel, Iztok
AU  - Djuran, Miloš
AU  - Glišić, Biljana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1789
AB  - Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.
PB  - Royal Society of Chemistry
T2  - RSC Advances
T2  - RSC Advances
T1  - Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex
EP  - 4393
IS  - 7
SP  - 4376
VL  - 13
DO  - 10.1039/D2RA07401J
ER  - 

@article{
author = "Andrejević, Tina and Aleksić, Ivana and Kljun, Jakob and Počkaj, Marta and Zlatar, Matija and Vojnović, Sandra and Nikodinović-Runić, Jasmina and Turel, Iztok and Djuran, Miloš and Glišić, Biljana",
year = "2023",
abstract = "Dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz) was used as a ligand for the synthesis of new copper(II) and silver(I) complexes, [CuCl2(py-2pz)]2 (1), [Cu(CF3SO3)(H2O)(py-2pz)2]CF3SO3·2H2O (2), [Ag(py-2pz)2]PF6 (3) and {[Ag(NO3)(py-2pz)]·0.5H2O}n (4). The complexes were characterized by spectroscopic and electrochemical methods, while their structures were determined by single crystal X-ray diffraction analysis. The X-ray analysis revealed the bidentate coordination mode of py-2pz to the corresponding metal ion via its pyridine and pyrazine nitrogen atoms in all complexes, while in polynuclear complex 4, the heterocyclic pyrazine ring of one py-2pz additionally behaves as a bridging ligand between two Ag(I) ions. DFT calculations were performed to elucidate the structures of the investigated complexes in solution. The antimicrobial potential of the complexes 1–4 was evaluated against two bacterial (Pseudomonas aeruginosa and Staphylococcus aureus) and two Candida (C. albicans and C. parapsilosis) species. Silver(I) complexes 3 and 4 have shown good antibacterial and antifungal properties with minimal inhibitory concentration (MIC) values ranging from 4.9 to 39.0 μM (3.9–31.2 μg mL−1). All complexes inhibited the filamentation of C. albicans and hyphae formation, while silver(I) complexes 3 and 4 had also the ability to inhibit the biofilm formation process of this fungus. The binding affinity of the complexes 1–4 with calf thymus DNA (ct-DNA) and bovine serum albumin (BSA) was studied by fluorescence emission spectroscopy to clarify the mode of their antimicrobial activity. Catechol oxidase biomimetic catalytic activity of copper(II) complexes 1 and 2 was additionally investigated by using 3,5-di-tert-butylcatechol (3,5-DTBC) and o-aminophenol (OAP) as substrates.",
publisher = "Royal Society of Chemistry",
journal = "RSC Advances, RSC Advances",
title = "Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex",
pages = "4393-4376",
number = "7",
volume = "13",
doi = "10.1039/D2RA07401J"
}

Andrejević, T., Aleksić, I., Kljun, J., Počkaj, M., Zlatar, M., Vojnović, S., Nikodinović-Runić, J., Turel, I., Djuran, M.,& Glišić, B.. (2023). Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex. in RSC Advances
Royal Society of Chemistry., 13(7), 4376-4393.
https://doi.org/10.1039/D2RA07401J

Andrejević T, Aleksić I, Kljun J, Počkaj M, Zlatar M, Vojnović S, Nikodinović-Runić J, Turel I, Djuran M, Glišić B. Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex. in RSC Advances. 2023;13(7):4376-4393.
doi:10.1039/D2RA07401J .

Andrejević, Tina, Aleksić, Ivana, Kljun, Jakob, Počkaj, Marta, Zlatar, Matija, Vojnović, Sandra, Nikodinović-Runić, Jasmina, Turel, Iztok, Djuran, Miloš, Glišić, Biljana, "Copper(II) and silver(I) complexes with dimethyl 6-(pyrazine-2-yl)pyridine-3,4-dicarboxylate (py-2pz): the influence of the metal ion on the antimicrobial potential of the complex" in RSC Advances, 13, no. 7 (2023):4376-4393,
https://doi.org/10.1039/D2RA07401J . .

TY  - JOUR
AU  - Hertrampf, Gesa
AU  - Vojnović, Sandra
AU  - Müller, Jonas I.
AU  - Merten, Christian
AU  - Nikodinović-Runić, Jasmina
AU  - Gulder, Tobias A. M.
PY  - 2023
UR  - https://doi.org/10.1021/acs.joc.3c01285
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2153
AB  - The myxobacterial natural product myxocoumarin A from Stigmatella aurantiaca MYX-030 has remarkable antifungal activity against agriculturally relevant pathogens. To broaden the initial evaluation of its biological potential, we herein completed the first total synthesis of myxocoumarin A. This synthetic access facilitated stereochemical investigations on the natural product structure, revealing its (R)-configuration. Biological activity profiling showed a lack of activity against Candida spp. and Gram-negative bacteria but revealed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus, including MRSA.
T2  - The Journal of Organic Chemistry
T1  - Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A
EP  - 14188
IS  - 19
SP  - 14184
VL  - 88
DO  - 10.1021/acs.joc.3c01285
ER  - 

@article{
author = "Hertrampf, Gesa and Vojnović, Sandra and Müller, Jonas I. and Merten, Christian and Nikodinović-Runić, Jasmina and Gulder, Tobias A. M.",
year = "2023",
abstract = "The myxobacterial natural product myxocoumarin A from Stigmatella aurantiaca MYX-030 has remarkable antifungal activity against agriculturally relevant pathogens. To broaden the initial evaluation of its biological potential, we herein completed the first total synthesis of myxocoumarin A. This synthetic access facilitated stereochemical investigations on the natural product structure, revealing its (R)-configuration. Biological activity profiling showed a lack of activity against Candida spp. and Gram-negative bacteria but revealed strong antibiotic activities against Bacillus subtilis and Staphylococcus aureus, including MRSA.",
journal = "The Journal of Organic Chemistry",
title = "Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A",
pages = "14188-14184",
number = "19",
volume = "88",
doi = "10.1021/acs.joc.3c01285"
}

Hertrampf, G., Vojnović, S., Müller, J. I., Merten, C., Nikodinović-Runić, J.,& Gulder, T. A. M.. (2023). Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A. in The Journal of Organic Chemistry, 88(19), 14184-14188.
https://doi.org/10.1021/acs.joc.3c01285

Hertrampf G, Vojnović S, Müller JI, Merten C, Nikodinović-Runić J, Gulder TAM. Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A. in The Journal of Organic Chemistry. 2023;88(19):14184-14188.
doi:10.1021/acs.joc.3c01285 .

Hertrampf, Gesa, Vojnović, Sandra, Müller, Jonas I., Merten, Christian, Nikodinović-Runić, Jasmina, Gulder, Tobias A. M., "Synthesis, Stereochemical Determination, and Antimicrobial Evaluation of Myxocoumarin A" in The Journal of Organic Chemistry, 88, no. 19 (2023):14184-14188,
https://doi.org/10.1021/acs.joc.3c01285 . .

TY  - JOUR
AU  - Mitrović, Aleksandra
AU  - Milovanović, Jelena
AU  - Gurgul, Jacek
AU  - Žekić, Andrijana
AU  - Nikodinović-Runić, Jasmina
AU  - Maslak, Veselin
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S0141022923001011
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1984
AB  - We present a novel approach for the enzymatic functionalization of graphene, utilizing horseradish peroxidase (HPO) and laccase (LC) from Trametes versicolor. This study demonstrates, for the first time, the covalent modification of non-homogeneous graphene with a low surface-to-volume ratio, both in solution and on solid support. Through thermogravimetry analysis, we estimate the degree of functionalization to be 11% with HPO and 4% with LC, attributed to the varying redox potentials of the enzymes. This work highlights the potential of enzymatic reactions for tailored functionalization of graphene under mild conditions.
PB  - Elsevier
T2  - Enzyme and Microbial Technology
T1  - Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase
SP  - 110293
VL  - 170
DO  - 10.1016/j.enzmictec.2023.110293
ER  - 

@article{
author = "Mitrović, Aleksandra and Milovanović, Jelena and Gurgul, Jacek and Žekić, Andrijana and Nikodinović-Runić, Jasmina and Maslak, Veselin",
year = "2023",
abstract = "We present a novel approach for the enzymatic functionalization of graphene, utilizing horseradish peroxidase (HPO) and laccase (LC) from Trametes versicolor. This study demonstrates, for the first time, the covalent modification of non-homogeneous graphene with a low surface-to-volume ratio, both in solution and on solid support. Through thermogravimetry analysis, we estimate the degree of functionalization to be 11% with HPO and 4% with LC, attributed to the varying redox potentials of the enzymes. This work highlights the potential of enzymatic reactions for tailored functionalization of graphene under mild conditions.",
publisher = "Elsevier",
journal = "Enzyme and Microbial Technology",
title = "Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase",
pages = "110293",
volume = "170",
doi = "10.1016/j.enzmictec.2023.110293"
}

Mitrović, A., Milovanović, J., Gurgul, J., Žekić, A., Nikodinović-Runić, J.,& Maslak, V.. (2023). Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase. in Enzyme and Microbial Technology
Elsevier., 170, 110293.
https://doi.org/10.1016/j.enzmictec.2023.110293

Mitrović A, Milovanović J, Gurgul J, Žekić A, Nikodinović-Runić J, Maslak V. Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase. in Enzyme and Microbial Technology. 2023;170:110293.
doi:10.1016/j.enzmictec.2023.110293 .

Mitrović, Aleksandra, Milovanović, Jelena, Gurgul, Jacek, Žekić, Andrijana, Nikodinović-Runić, Jasmina, Maslak, Veselin, "Enzymatic functionalization of liquid phase exfoliated graphene using horseradish peroxidase and laccase" in Enzyme and Microbial Technology, 170 (2023):110293,
https://doi.org/10.1016/j.enzmictec.2023.110293 . .

TY  - CONF
AU  - Lončarević, B.
AU  - Lješević, M.
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Gojgić-Cvijović, G.
AU  - Beškoski, V.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2174
AB  - Niska cena i jednostavna proizvodnja sintetičkih plastičnih materijala dovela je do
njihove raširene upotrebe za različita pakovanja, građevinarstvo, automobilsku i elektronsku
industriju i izradu proizvoda za domaćinstva. Zbog toga se proizvodnja plastike u poslednjih
pola veka povećala 20 puta [1]. Najviše se koristi polietilen tereftalat (PET), a procenjeno je
da je njegova proizvodnja 2022. godine dostigla 87,17 miliona kubnih metara. Visoka
otpornost PET-a, koja se ranije smatrala prednošću, danas dovodi do nekontrolisanog
nagomilavanja otpada u brojnim ekosistemima na planeti [2]. Bis-(2-hidroksietil)-tereftalat
(BHET) je komercijalni monomersa strukturom sličnom jezgru PET-a, pa se široko koristi
u ispitivanjima biodegradacije PET-a, jer je pokazano da pojedini mikroorganizmi imaju
sposobnost da ga degraduju [3]. Osim mogućnosti razgradnje različitih štetnih jedinjenja,
mikroorganizmi mogu da proizvode i egzopolisaharide (EPS), kao odgovor na različite
selektivne pritiske životne sredine. EPS su zbog svojih fizičko-hemijskih svojstava našli
primenu u farmaceutskoj, hemijskoj i prehrambenoj industriji [4].
Cilj ovog rada bio je selektovati mikroorganizme koji mogu da razgrade BHET kao
model jedinjenje za ispitivanje degradacije plastičnog otpada na bazi PET-a i sa visokim
prinosom produkuju EPS.
Sposobnost degradacije BHET-a ispitivana je na 100 bakterijskih izolata, pomoću
čvrstih podloga sa BHET-om kao glavnim izvorom ugljenika. Potom je selektovano 7 sojeva
sa najširim zonama prosvetljenja, koji su korišćeni za produkciju EPS-a u tečnim podlogama
sa saharozom. Nakon fermentacije i odvajanja biomase, EPS su taloženi dvostrukom
zapreminom etanola i njihova količina je određena gravimetrijskom metodom [5]. Masa
dobijenih EPS varirala je od 2,4 – 33,7 g/L, a odabran je soj sa visokom produkcijom za
dalje eksperimente. Pošto mikrobna produkcija polisaharida veoma zavisi od uslova
fermentacije, ispitan je uticaj pet faktora na prinos: temperature, aeracije, pH, količine
saharoze i različitih izvora azota. Rezultati su pokazali da najoptimalnija podloga za
dobijanje visokog prinosa EPS-a sadrži ekstrakt kvasca (izvor azota), 100 g/L saharoze
(izvor ugljenika), a pH vrednost joj je 7.
Budući ekperimenti će biti usmereni na ispitivanje interakcija između ključnih faktora
na proizvodnju EPS-a, radi naprednije optimizacije procesa i povećanja prinosa.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem
T1  - Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije
T1  - Exopolysaccharides from microorganisms which degrade BHET: Optimization of production
EP  - 46
SP  - 45
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2174
ER  - 

@conference{
author = "Lončarević, B. and Lješević, M. and Joksimović, K. and Žerađanin, A. and Gojgić-Cvijović, G. and Beškoski, V. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Niska cena i jednostavna proizvodnja sintetičkih plastičnih materijala dovela je do
njihove raširene upotrebe za različita pakovanja, građevinarstvo, automobilsku i elektronsku
industriju i izradu proizvoda za domaćinstva. Zbog toga se proizvodnja plastike u poslednjih
pola veka povećala 20 puta [1]. Najviše se koristi polietilen tereftalat (PET), a procenjeno je
da je njegova proizvodnja 2022. godine dostigla 87,17 miliona kubnih metara. Visoka
otpornost PET-a, koja se ranije smatrala prednošću, danas dovodi do nekontrolisanog
nagomilavanja otpada u brojnim ekosistemima na planeti [2]. Bis-(2-hidroksietil)-tereftalat
(BHET) je komercijalni monomersa strukturom sličnom jezgru PET-a, pa se široko koristi
u ispitivanjima biodegradacije PET-a, jer je pokazano da pojedini mikroorganizmi imaju
sposobnost da ga degraduju [3]. Osim mogućnosti razgradnje različitih štetnih jedinjenja,
mikroorganizmi mogu da proizvode i egzopolisaharide (EPS), kao odgovor na različite
selektivne pritiske životne sredine. EPS su zbog svojih fizičko-hemijskih svojstava našli
primenu u farmaceutskoj, hemijskoj i prehrambenoj industriji [4].
Cilj ovog rada bio je selektovati mikroorganizme koji mogu da razgrade BHET kao
model jedinjenje za ispitivanje degradacije plastičnog otpada na bazi PET-a i sa visokim
prinosom produkuju EPS.
Sposobnost degradacije BHET-a ispitivana je na 100 bakterijskih izolata, pomoću
čvrstih podloga sa BHET-om kao glavnim izvorom ugljenika. Potom je selektovano 7 sojeva
sa najširim zonama prosvetljenja, koji su korišćeni za produkciju EPS-a u tečnim podlogama
sa saharozom. Nakon fermentacije i odvajanja biomase, EPS su taloženi dvostrukom
zapreminom etanola i njihova količina je određena gravimetrijskom metodom [5]. Masa
dobijenih EPS varirala je od 2,4 – 33,7 g/L, a odabran je soj sa visokom produkcijom za
dalje eksperimente. Pošto mikrobna produkcija polisaharida veoma zavisi od uslova
fermentacije, ispitan je uticaj pet faktora na prinos: temperature, aeracije, pH, količine
saharoze i različitih izvora azota. Rezultati su pokazali da najoptimalnija podloga za
dobijanje visokog prinosa EPS-a sadrži ekstrakt kvasca (izvor azota), 100 g/L saharoze
(izvor ugljenika), a pH vrednost joj je 7.
Budući ekperimenti će biti usmereni na ispitivanje interakcija između ključnih faktora
na proizvodnju EPS-a, radi naprednije optimizacije procesa i povećanja prinosa.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem",
title = "Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije, Exopolysaccharides from microorganisms which degrade BHET: Optimization of production",
pages = "46-45",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2174"
}

Lončarević, B., Lješević, M., Joksimović, K., Žerađanin, A., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem
Beograd : Srpsko hemijsko društvo., 9, 45-46.
https://hdl.handle.net/21.15107/rcub_imagine_2174

Lončarević B, Lješević M, Joksimović K, Žerađanin A, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem. 2023;9:45-46.
https://hdl.handle.net/21.15107/rcub_imagine_2174 .

Lončarević, B., Lješević, M., Joksimović, K., Žerađanin, A., Gojgić-Cvijović, G., Beškoski, V., Nikodinović-Runić, Jasmina, "Egzopolisaharidi mikroorganizama koji mogu da degraduju BHET: Optimizacija produkcije" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem, 9 (2023):45-46,
https://hdl.handle.net/21.15107/rcub_imagine_2174 .

TY  - CONF
AU  - Lazić, Jelena
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.chem.bg.ac.rs/pz/news1.py?q=3820&l=1
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2205
AB  - Microorganisms, our planet’s original inhabitants discovered with the invention of the
first microscope in the 17th century, have consistently facilitated our daily life. However,
our modern life generates enormous amounts of wastes, such as plastic, food, and
chemicals from the pharmaceutical industry. Bacterial natural products hold an
important position in this industry, as drug leads in synthetic chemistry and biology,
essential for the discovery of effective agents against a variety of human diseases. If the
existing waste is used as a nutrient source for microbial production of valuable
biomolecules, that concept is called “waste to value” or “upcycling”.
This concept was explored using bacterial biopigment prodigiosin (PG, Fig. 1) as part of
the BioECOLogics project. This proof of concept demonstrates how the bacteria Serratia
marcescens ATCC 27117 can use a waste stream from the food industry as a carbon
source to grow and produce its bioactive secondary metabolite PG. The unique structure
of this molecule was changed through green chemical [1] and biopolymer formulation
[2] approaches, as well as metal complexation. Finally, these sustainable biotherapeutics
were validated in vitro (antimicrobial, anticancer) and in vivo (nematode Caenorhabditis
elegans and zebrafish Danio rerio).
PB  - Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club
C3  - 9th Conference of Young Chemists of Serbia
T1  - From waste streams to biotherapeutics: making a connection using bacteria
EP  - 6
SP  - 6
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2205
ER  - 

@conference{
author = "Lazić, Jelena and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Microorganisms, our planet’s original inhabitants discovered with the invention of the
first microscope in the 17th century, have consistently facilitated our daily life. However,
our modern life generates enormous amounts of wastes, such as plastic, food, and
chemicals from the pharmaceutical industry. Bacterial natural products hold an
important position in this industry, as drug leads in synthetic chemistry and biology,
essential for the discovery of effective agents against a variety of human diseases. If the
existing waste is used as a nutrient source for microbial production of valuable
biomolecules, that concept is called “waste to value” or “upcycling”.
This concept was explored using bacterial biopigment prodigiosin (PG, Fig. 1) as part of
the BioECOLogics project. This proof of concept demonstrates how the bacteria Serratia
marcescens ATCC 27117 can use a waste stream from the food industry as a carbon
source to grow and produce its bioactive secondary metabolite PG. The unique structure
of this molecule was changed through green chemical [1] and biopolymer formulation
[2] approaches, as well as metal complexation. Finally, these sustainable biotherapeutics
were validated in vitro (antimicrobial, anticancer) and in vivo (nematode Caenorhabditis
elegans and zebrafish Danio rerio).",
publisher = "Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club",
journal = "9th Conference of Young Chemists of Serbia",
title = "From waste streams to biotherapeutics: making a connection using bacteria",
pages = "6-6",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2205"
}

Lazić, J.,& Nikodinović-Runić, J.. (2023). From waste streams to biotherapeutics: making a connection using bacteria. in 9th Conference of Young Chemists of Serbia
Belgrade : Serbian Chemical Society and Serbian Young Chemists’ Club., 6-6.
https://hdl.handle.net/21.15107/rcub_imagine_2205

Lazić J, Nikodinović-Runić J. From waste streams to biotherapeutics: making a connection using bacteria. in 9th Conference of Young Chemists of Serbia. 2023;:6-6.
https://hdl.handle.net/21.15107/rcub_imagine_2205 .

Lazić, Jelena, Nikodinović-Runić, Jasmina, "From waste streams to biotherapeutics: making a connection using bacteria" in 9th Conference of Young Chemists of Serbia (2023):6-6,
https://hdl.handle.net/21.15107/rcub_imagine_2205 .

TY  - JOUR
AU  - Ponjavić, Marijana
AU  - Malagurski, Ivana
AU  - Lazić, Jelena
AU  - Jeremić, Sanja
AU  - Pavlović, Vladimir
AU  - Prlainović, Nevena
AU  - Maksimović, Vesna
AU  - Cosović, Vladan
AU  - Atanase, Leonard Ionut
AU  - Freitas, Filomena
AU  - Matos, Mariana
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1714
AB  - The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin
IS  - 3
SP  - 1906
VL  - 24
DO  - 10.3390/ijms24031906
ER  - 

@article{
author = "Ponjavić, Marijana and Malagurski, Ivana and Lazić, Jelena and Jeremić, Sanja and Pavlović, Vladimir and Prlainović, Nevena and Maksimović, Vesna and Cosović, Vladan and Atanase, Leonard Ionut and Freitas, Filomena and Matos, Mariana and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "The quest for sustainable biomaterials with excellent biocompatibility and tailorable properties has put polyhydroxyalkanoates (PHAs) into the research spotlight. However, high production costs and the lack of bioactivity limit their market penetration. To address this, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was combined with a bacterial pigment with strong anticancer activity, prodigiosin (PG), to obtain functionally enhanced PHBV-based biomaterials. The samples were produced in the form of films 115.6–118.8 µm in thickness using the solvent casting method. The effects of PG incorporation on the physical properties (morphology, biopolymer crystallinity and thermal stability) and functionality of the obtained biomaterials were investigated. PG has acted as a nucleating agent, in turn affecting the degree of crystallinity, thermal stability and morphology of the films. All samples with PG had a more organized internal structure and higher melting and degradation temperatures. The calculated degree of crystallinity of the PHBV copolymer was 53%, while the PG1, PG3 and PG3 films had values of 64.0%, 63.9% and 69.2%, respectively. Cytotoxicity studies have shown the excellent anticancer activity of films against HCT116 (colon cancer) cells, thus advancing PHBV biomedical application potential.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin",
number = "3",
pages = "1906",
volume = "24",
doi = "10.3390/ijms24031906"
}

Ponjavić, M., Malagurski, I., Lazić, J., Jeremić, S., Pavlović, V., Prlainović, N., Maksimović, V., Cosović, V., Atanase, L. I., Freitas, F., Matos, M.,& Nikodinović-Runić, J.. (2023). Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences, 24(3), 1906.
https://doi.org/10.3390/ijms24031906

Ponjavić M, Malagurski I, Lazić J, Jeremić S, Pavlović V, Prlainović N, Maksimović V, Cosović V, Atanase LI, Freitas F, Matos M, Nikodinović-Runić J. Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin. in International Journal of Molecular Sciences. 2023;24(3):1906.
doi:10.3390/ijms24031906 .

Ponjavić, Marijana, Malagurski, Ivana, Lazić, Jelena, Jeremić, Sanja, Pavlović, Vladimir, Prlainović, Nevena, Maksimović, Vesna, Cosović, Vladan, Atanase, Leonard Ionut, Freitas, Filomena, Matos, Mariana, Nikodinović-Runić, Jasmina, "Advancing PHBV Biomedical Potential with the Incorporation of Bacterial Biopigment Prodigiosin" in International Journal of Molecular Sciences, 24, no. 3 (2023):1906,
https://doi.org/10.3390/ijms24031906 . .

TY  - CONF
AU  - Lješević, M.
AU  - Lončarević, B.
AU  - Joksimović, K.
AU  - Žerađanin, A.
AU  - Pantelić, Brana
AU  - Gojgić-Cvijović, G.
AU  - Beškoski, V.
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2173
AB  - Proizvodnja plastike i zamena staklene i keramičke ambalaže plastičnim materijalima
doveli su do nagomilavanja plastičnog otpada. Neophodno je naći povoljan sistem za
degradaciju plastičnog otpada, bez nastanka toksičnih produkata ili dodatnog zagađenja
životne sredine. Polietilen-tereftalat (PET) je jedan od najčešće proizvedenih plastičnih
polimera. Proizvodnja PET-a započinje esterifikacijom tereftalne kiseline i etilen glikola,
pri čemu nastaje bis-(2-hidroksietil)-tereftalat (BHET), koji se dalje polikondenzuje do
polimera. U poslednje vreme, BHET se često koristi kao model jedinjenje za identifikovanje
novih biokatalizatora za degradaciju PET-a [1,2].
Cilj ovog rada bio je ispitivanje mehanizma degradacije BHET-a pomoću
mikroorganizama.
U preliminarnom testu na čvrstim podlogama, kapacitet za degradaciju BHET-a je
testiran kod stotinak mikroorganizama, nakon čega su odabrani najefikasniji sojevi, koji su
identifikovani sekvenciranjem gena za 16s rRNK. Dalje, ispitivana je degradacija u tečnoj
podlozi gde je BHET bio glavni izvor ugljenika. Eksperiment je trajao 7 dana, a degradacija
je praćena nakon drugog, petog i sedmog dana upotrebom tečne hromatografije (HPLC).
Kao najefikasniji sojevi pokazali su se pripadnici roda Pseudomonas. Oni su u potpunosti
transformisali BHET do različitih intermedijera.
Rezultati su pokazali da ispitivani sojevi mogu da transformišu BHET, korišćenjem
najmanje dva različita puta, pa će se naredni eksperimenti usmeriti na identifikaciju
intermedijera degradacije. Takođe, radi optimizacije degradacije, ispitivaće se simbiotsko i
sinergističko dejstvo različitih konzorcijuma, kako bi se obezbedila potpuna degradacija
ovog model jedinjenja.
PB  - Beograd : Srpsko hemijsko društvo
C3  - 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
T1  - Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata
T1  - Microbial degradation of bis (2-hydroxyethyl) terephthalate
EP  - 44
SP  - 43
VL  - 9
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2173
ER  - 

@conference{
author = "Lješević, M. and Lončarević, B. and Joksimović, K. and Žerađanin, A. and Pantelić, Brana and Gojgić-Cvijović, G. and Beškoski, V. and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Proizvodnja plastike i zamena staklene i keramičke ambalaže plastičnim materijalima
doveli su do nagomilavanja plastičnog otpada. Neophodno je naći povoljan sistem za
degradaciju plastičnog otpada, bez nastanka toksičnih produkata ili dodatnog zagađenja
životne sredine. Polietilen-tereftalat (PET) je jedan od najčešće proizvedenih plastičnih
polimera. Proizvodnja PET-a započinje esterifikacijom tereftalne kiseline i etilen glikola,
pri čemu nastaje bis-(2-hidroksietil)-tereftalat (BHET), koji se dalje polikondenzuje do
polimera. U poslednje vreme, BHET se često koristi kao model jedinjenje za identifikovanje
novih biokatalizatora za degradaciju PET-a [1,2].
Cilj ovog rada bio je ispitivanje mehanizma degradacije BHET-a pomoću
mikroorganizama.
U preliminarnom testu na čvrstim podlogama, kapacitet za degradaciju BHET-a je
testiran kod stotinak mikroorganizama, nakon čega su odabrani najefikasniji sojevi, koji su
identifikovani sekvenciranjem gena za 16s rRNK. Dalje, ispitivana je degradacija u tečnoj
podlozi gde je BHET bio glavni izvor ugljenika. Eksperiment je trajao 7 dana, a degradacija
je praćena nakon drugog, petog i sedmog dana upotrebom tečne hromatografije (HPLC).
Kao najefikasniji sojevi pokazali su se pripadnici roda Pseudomonas. Oni su u potpunosti
transformisali BHET do različitih intermedijera.
Rezultati su pokazali da ispitivani sojevi mogu da transformišu BHET, korišćenjem
najmanje dva različita puta, pa će se naredni eksperimenti usmeriti na identifikaciju
intermedijera degradacije. Takođe, radi optimizacije degradacije, ispitivaće se simbiotsko i
sinergističko dejstvo različitih konzorcijuma, kako bi se obezbedila potpuna degradacija
ovog model jedinjenja.",
publisher = "Beograd : Srpsko hemijsko društvo",
journal = "9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023",
title = "Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata, Microbial degradation of bis (2-hydroxyethyl) terephthalate",
pages = "44-43",
volume = "9",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2173"
}

Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Pantelić, B., Gojgić-Cvijović, G., Beškoski, V.,& Nikodinović-Runić, J.. (2023). Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023
Beograd : Srpsko hemijsko društvo., 9, 43-44.
https://hdl.handle.net/21.15107/rcub_imagine_2173

Lješević M, Lončarević B, Joksimović K, Žerađanin A, Pantelić B, Gojgić-Cvijović G, Beškoski V, Nikodinović-Runić J. Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata. in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023. 2023;9:43-44.
https://hdl.handle.net/21.15107/rcub_imagine_2173 .

Lješević, M., Lončarević, B., Joksimović, K., Žerađanin, A., Pantelić, Brana, Gojgić-Cvijović, G., Beškoski, V., Nikodinović-Runić, Jasmina, "Mikrobiološka degradacija bis (2-hidroksietil)-tereftalata" in 9. simpozijum Hemija i zaštita životne sredine sa međunarodnim učešćem, EnviroChem2023, 9 (2023):43-44,
https://hdl.handle.net/21.15107/rcub_imagine_2173 .

TY  - JOUR
AU  - Pantelić, Brana
AU  - Araujo, Jeovan
AU  - Jeremić, Sanja
AU  - Azeem, Muhammad
AU  - Attallah, Olivia
AU  - Slaperas, Romanos
AU  - Mojicević, Marija
AU  - Chen, Yuanyuan
AU  - Fournet, Margaret Brennan
AU  - Topakas, Evangelos
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://www.sciencedirect.com/science/article/pii/S2352186423003127
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1983
AB  - Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.
PB  - Elsevier
T2  - Environmental Technology & Innovation
T1  - A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate
SP  - 103316
DO  - 10.1016/j.eti.2023.103316
ER  - 

@article{
author = "Pantelić, Brana and Araujo, Jeovan and Jeremić, Sanja and Azeem, Muhammad and Attallah, Olivia and Slaperas, Romanos and Mojicević, Marija and Chen, Yuanyuan and Fournet, Margaret Brennan and Topakas, Evangelos and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Biotechnological treatment of plastic waste has gathered substantial attention as an efficient and generally greener approach for polyethylene terephthalate (PET) depolymerization and upcycling in comparison to mechanical and chemical processes. Nevertheless, a suitable combination of mechanical and microbial degradation may be the key to bringing forward PET upcycling. In this study, a new strain with an excellent bis(2 hydroxyethyl)terephthalate (BHET) degradation potential (1000 mg/mL in 120 h at 30 °C) and wide temperature (20-47 °C) and pH (5-10) tolerance was isolated from a pristine soil sample. It was identified as Bacillus subtilis BPM12 via phenotypical and genome analysis. A number of enzymes with potential polymer degrading activities were identified, including carboxylesterase BPM12CE that was efficiently expressed both, homologously in B. subtilis BPM12 and heterologously in B. subtilis 168 strain. Overexpression of this enzyme enabled B. subtilis 168 to degrade BHET, while the activity of BPM12 increased up to 1.8-fold, confirming its BHET-ase activity. Interaction of B. subtilis BPM12 with virgin PET films and films that were re-extruded up to 5 times mimicking mechanical recycling, revealed the ability of the strain to attach and form biofilm on each surface. Mechanical recycling resulted in PET materials that are more susceptible to chemical hydrolysis, however only slight differences were detected in biological degradation when BPM12 whole-cells or cell-free enzyme preparations were used. Mixed mechano/bio-degradation with whole-cells and crude enzyme mixes from this strain can serve to further increase the percentage of PET- based plastics that can enter circularity.",
publisher = "Elsevier",
journal = "Environmental Technology & Innovation",
title = "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate",
pages = "103316",
doi = "10.1016/j.eti.2023.103316"
}

Pantelić, B., Araujo, J., Jeremić, S., Azeem, M., Attallah, O., Slaperas, R., Mojicević, M., Chen, Y., Fournet, M. B., Topakas, E.,& Nikodinović-Runić, J.. (2023). A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation
Elsevier., 103316.
https://doi.org/10.1016/j.eti.2023.103316

Pantelić B, Araujo J, Jeremić S, Azeem M, Attallah O, Slaperas R, Mojicević M, Chen Y, Fournet MB, Topakas E, Nikodinović-Runić J. A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate. in Environmental Technology & Innovation. 2023;:103316.
doi:10.1016/j.eti.2023.103316 .

Pantelić, Brana, Araujo, Jeovan, Jeremić, Sanja, Azeem, Muhammad, Attallah, Olivia, Slaperas, Romanos, Mojicević, Marija, Chen, Yuanyuan, Fournet, Margaret Brennan, Topakas, Evangelos, Nikodinović-Runić, Jasmina, "A novel Bacillus subtilis BPM12 with high bis(2 hydroxyethyl)terephthalate hydrolytic activity efficiently interacts with virgin and mechanically recycled polyethylene terephthalate" in Environmental Technology & Innovation (2023):103316,
https://doi.org/10.1016/j.eti.2023.103316 . .

TY  - CONF
AU  - Rajesekhran, Divya
AU  - Ponnupandian, Siva
AU  - Bhagabati, Purabi
AU  - Milovanović, Jelena
AU  - Nikodinović-Runić, Jasmina
AU  - Babu, Ramesh
PY  - 2023
UR  - https://pps2023india.com/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2286
AB  - In a world where unsustainable anthropogenic activities are drastically affecting Earth’s
ecosystems and natural resources, threatening animal (including human) health and safety, the
sustainable management and use of resources, including preventing plastic pollution, have
become of paramount importance. Plastics, being at the core of many low cost and high
performing materials, are widely used despite being based on non-renewable petrochemicals.
Currently, in EU, only 7% of plastics are recycled out of 57.2 million tonnes produced, and
93% end up in incineration, landfills and oceans, releasing persistent, bio-accumulative, toxic
and hazardous chemicals.
Bio Innovation of a Circular Economy for Plastics 1 (BioICEP) an EU Horizon project focuses
on reducing the burden of plastic waste in the environment and also changing the linear value
chains of current plastic products. The project's overall objective is to demonstrate a seamless,
sustainable route to a circular economy for plastics by developing cost-effective and low-
energy demand processes for waste plastic biotransformation into high-value marketable
products such as biodegradable plastics. To address these challenges, the BioICEP project
focuses on developing physical, enzymatic and biocatalytic pre-treatments to convert the
mixed plastic waste to biodegradable plastics focusing on recycling and upcycling of the
products with a zero-waste and regenerative approach.
C3  - Polymer Processing Society Asia-Australasia Regional Conference
T1  - Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2286
ER  - 

@conference{
author = "Rajesekhran, Divya and Ponnupandian, Siva and Bhagabati, Purabi and Milovanović, Jelena and Nikodinović-Runić, Jasmina and Babu, Ramesh",
year = "2023",
abstract = "In a world where unsustainable anthropogenic activities are drastically affecting Earth’s
ecosystems and natural resources, threatening animal (including human) health and safety, the
sustainable management and use of resources, including preventing plastic pollution, have
become of paramount importance. Plastics, being at the core of many low cost and high
performing materials, are widely used despite being based on non-renewable petrochemicals.
Currently, in EU, only 7% of plastics are recycled out of 57.2 million tonnes produced, and
93% end up in incineration, landfills and oceans, releasing persistent, bio-accumulative, toxic
and hazardous chemicals.
Bio Innovation of a Circular Economy for Plastics 1 (BioICEP) an EU Horizon project focuses
on reducing the burden of plastic waste in the environment and also changing the linear value
chains of current plastic products. The project's overall objective is to demonstrate a seamless,
sustainable route to a circular economy for plastics by developing cost-effective and low-
energy demand processes for waste plastic biotransformation into high-value marketable
products such as biodegradable plastics. To address these challenges, the BioICEP project
focuses on developing physical, enzymatic and biocatalytic pre-treatments to convert the
mixed plastic waste to biodegradable plastics focusing on recycling and upcycling of the
products with a zero-waste and regenerative approach.",
journal = "Polymer Processing Society Asia-Australasia Regional Conference",
title = "Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2286"
}

Rajesekhran, D., Ponnupandian, S., Bhagabati, P., Milovanović, J., Nikodinović-Runić, J.,& Babu, R.. (2023). Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics. in Polymer Processing Society Asia-Australasia Regional Conference.
https://hdl.handle.net/21.15107/rcub_imagine_2286

Rajesekhran D, Ponnupandian S, Bhagabati P, Milovanović J, Nikodinović-Runić J, Babu R. Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics. in Polymer Processing Society Asia-Australasia Regional Conference. 2023;.
https://hdl.handle.net/21.15107/rcub_imagine_2286 .

Rajesekhran, Divya, Ponnupandian, Siva, Bhagabati, Purabi, Milovanović, Jelena, Nikodinović-Runić, Jasmina, Babu, Ramesh, "Circular Plastics: Upscaling the mixed plastic waste to biodegradable  plastics" in Polymer Processing Society Asia-Australasia Regional Conference (2023),
https://hdl.handle.net/21.15107/rcub_imagine_2286 .

TY  - CONF
AU  - Ćirić, Milica
AU  - Pantelić, Brana
AU  - Šaraba, Vladimir
AU  - Nikodinović-Runić, Jasmina
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2044
AB  - Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous
synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the
soil and water environments. Highly efficient PU-degrading microorganisms are rare in
nature and are of fundamental importance for achieving circular plastic economy. Bacterial
isolates from groundwater, originating from magmatogenic massif and Tertiary basin
within metamorphic area, as well as soil isolates collected from various pristine (PS) and
contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD
(IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA
gene sequences from IMP-degrading isolates was performed using the neighbor-joining
method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and
18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading
isolates from each type of environment, belonging to 8 genera (Pseudomonas,
Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas)
for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter,
Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No
clear grouping of groundwater and soil isolates was observed, indicating that isolates are
too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For
groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus,
2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering
was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping
within isolates from CS and PS. In the future, wider range of environmental niches should be
included in screening efforts for development of biocatalytic processes for management of
plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and
represent largely unexplored reservoir of biotechnologically relevant enzymatic activities,
should also be more represented in such screenings.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Groundwater and soil as a reservoir for polyurethane-degrading bacteria
EP  - 99
SP  - 99
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2044
ER  - 

@conference{
author = "Ćirić, Milica and Pantelić, Brana and Šaraba, Vladimir and Nikodinović-Runić, Jasmina",
year = "2023",
abstract = "Plastic waste is a global environmental burden. Polyurethanes (PU), toxic and ubiquitous
synthetic polymers, do not biodegrade quickly, leading to their rapid accumulation in the
soil and water environments. Highly efficient PU-degrading microorganisms are rare in
nature and are of fundamental importance for achieving circular plastic economy. Bacterial
isolates from groundwater, originating from magmatogenic massif and Tertiary basin
within metamorphic area, as well as soil isolates collected from various pristine (PS) and
contaminated sites (CS), were screened using PU model compound Impranil® DLN-SD
(IMP) as sole C source to identify PU-degrading isolates. Phylogenetic analysis of 16S rRNA
gene sequences from IMP-degrading isolates was performed using the neighbor-joining
method to observe their clustering. Thirty one of 96 isolates (32.3 %) from groundwater and
18 of 220 isolates (8.2%) from soil produced prominent IMP-clearing zones. Thirteen IMPdegrading
isolates from each type of environment, belonging to 8 genera (Pseudomonas,
Proteus, Enterobacter, Flavobacterium, Serratia, Pantoea, Acinetobacter and Stenotrophomonas)
for groundwater and to 6 genera (Streptomyces, Pseudomonas, Rhodococcus, Achromobacter,
Bacillus and Paenibacillus) for soil environment, were included in phylogenetic analysis. No
clear grouping of groundwater and soil isolates was observed, indicating that isolates are
too distinct. Stronger clustering was observed for groundwater compared to soil isolates. For
groundwater, strongest clustering was observed for 2 isolates belonging to Proteus genus,
2 belonging to Flavobacterium and 2 to Pseudomonas. For soil samples, strongest clustering
was observed for 3 isolates belonging to genus Streptomyces. There was no clear grouping
within isolates from CS and PS. In the future, wider range of environmental niches should be
included in screening efforts for development of biocatalytic processes for management of
plastic waste. Subterranean ecosystems, which are not readily accessible for sampling and
represent largely unexplored reservoir of biotechnologically relevant enzymatic activities,
should also be more represented in such screenings.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Groundwater and soil as a reservoir for polyurethane-degrading bacteria",
pages = "99-99",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2044"
}

Ćirić, M., Pantelić, B., Šaraba, V.,& Nikodinović-Runić, J.. (2023). Groundwater and soil as a reservoir for polyurethane-degrading bacteria. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2044

Ćirić M, Pantelić B, Šaraba V, Nikodinović-Runić J. Groundwater and soil as a reservoir for polyurethane-degrading bacteria. in 4th Belgrade Bioinformatics Conference. 2023;4:99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2044 .

Ćirić, Milica, Pantelić, Brana, Šaraba, Vladimir, Nikodinović-Runić, Jasmina, "Groundwater and soil as a reservoir for polyurethane-degrading bacteria" in 4th Belgrade Bioinformatics Conference, 4 (2023):99-99,
https://hdl.handle.net/21.15107/rcub_imagine_2044 .