TY  - JOUR
AU  - Balint, Vanda
AU  - Perić, Mina
AU  - Dačić, Sanja
AU  - Stanisavljević Ninković, Danijela
AU  - Marjanović, Jelena
AU  - Popović, Jelena
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2024
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2340
AB  - Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.
PB  - MDPI
T2  - Biomedicines
T1  - The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes
IS  - 4
SP  - 796
VL  - 12
DO  - 10.3390/biomedicines12040796
ER  - 

@article{
author = "Balint, Vanda and Perić, Mina and Dačić, Sanja and Stanisavljević Ninković, Danijela and Marjanović, Jelena and Popović, Jelena and Stevanović, Milena and Lazić, Andrijana",
year = "2024",
abstract = "Astrocytes are the main homeostatic cells in the central nervous system, with the unique ability to transform from quiescent into a reactive state in response to pathological conditions by reacquiring some precursor properties. This process is known as reactive astrogliosis, a compensatory response that mediates tissue damage and recovery. Although it is well known that SOX transcription factors drive the expression of phenotype-specific genetic programs during neurodevelopment, their roles in mature astrocytes have not been studied extensively. We focused on the transcription factors SOX2 and SOX9, shown to be re-expressed in reactive astrocytes, in order to study the reactivation-related functional properties of astrocytes mediated by those proteins. We performed an initial screening of SOX2 and SOX9 expression after sensorimotor cortex ablation injury in rats and conducted gain-of-function studies in vitro using astrocytes derived from the human NT2/D1 cell line. Our results revealed the direct involvement of SOX2 in the reacquisition of proliferation in mature NT2/D1-derived astrocytes, while SOX9 overexpression increased migratory potential and glutamate uptake in these cells. Our results imply that modulation of SOX gene expression may change the functional properties of astrocytes, which holds promise for the discovery of potential therapeutic targets in the development of novel strategies for tissue regeneration and recovery.",
publisher = "MDPI",
journal = "Biomedicines",
title = "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes",
number = "4",
pages = "796",
volume = "12",
doi = "10.3390/biomedicines12040796"
}

Balint, V., Perić, M., Dačić, S., Stanisavljević Ninković, D., Marjanović, J., Popović, J., Stevanović, M.,& Lazić, A.. (2024). The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines
MDPI., 12(4), 796.
https://doi.org/10.3390/biomedicines12040796

Balint V, Perić M, Dačić S, Stanisavljević Ninković D, Marjanović J, Popović J, Stevanović M, Lazić A. The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes. in Biomedicines. 2024;12(4):796.
doi:10.3390/biomedicines12040796 .

Balint, Vanda, Perić, Mina, Dačić, Sanja, Stanisavljević Ninković, Danijela, Marjanović, Jelena, Popović, Jelena, Stevanović, Milena, Lazić, Andrijana, "The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes" in Biomedicines, 12, no. 4 (2024):796,
https://doi.org/10.3390/biomedicines12040796 . .

TY  - JOUR
AU  - Rakonjac, Marijana
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Simeunović, Ivana
AU  - Kostić, Jovana
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2024
UR  - https://www.mdpi.com/2227-9067/11/4/489
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2362
AB  - 22q11.2 deletion syndrome (22q11.2DS), the most frequent microdeletion syndrome in humans, is related to a high risk of developing neurodevelopmental disorders. About 95% of patients with 22q11.2DS have speech and language impairments. Global articulation, story generation, and verbal memory tests were applied to compare articulatory characteristics of speech sounds, spontaneous language abilities, and immediate verbal memory between four groups of Serbian-speaking children: patients with 22q11.2DS, children with clinical presentation of 22q11.2DS that do not have the microdeletion, children with non-syndromic congenital heart defects, and their peers with typical speech–sound development. The obtained results showed that children with this microdeletion have impaired articulation skills and expressive language abilities. However, we did not observe weaker receptive language skills and immediate verbal memory compared to healthy controls. Children with 22q11.2DS should be considered a risk category for the development of speech–sound pathology and expressive language abilities. Since speech intelligibility is an instrument of cognition and adequate peer socialization, and language impairment in school-aged children with 22q11DS might be an indicator of increased risk for later psychotic symptoms, patients with 22q11.2 microdeletion should be included in a program of early stimulation of speech–language development immediately after diagnosis is established.
PB  - MDPI
T2  - Children
T2  - Children
T1  - Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion
IS  - 4
SP  - 489
VL  - 11
DO  - 10.3390/children11040489
ER  - 

@article{
author = "Rakonjac, Marijana and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Simeunović, Ivana and Kostić, Jovana and Stevanović, Milena and Drakulić, Danijela",
year = "2024",
abstract = "22q11.2 deletion syndrome (22q11.2DS), the most frequent microdeletion syndrome in humans, is related to a high risk of developing neurodevelopmental disorders. About 95% of patients with 22q11.2DS have speech and language impairments. Global articulation, story generation, and verbal memory tests were applied to compare articulatory characteristics of speech sounds, spontaneous language abilities, and immediate verbal memory between four groups of Serbian-speaking children: patients with 22q11.2DS, children with clinical presentation of 22q11.2DS that do not have the microdeletion, children with non-syndromic congenital heart defects, and their peers with typical speech–sound development. The obtained results showed that children with this microdeletion have impaired articulation skills and expressive language abilities. However, we did not observe weaker receptive language skills and immediate verbal memory compared to healthy controls. Children with 22q11.2DS should be considered a risk category for the development of speech–sound pathology and expressive language abilities. Since speech intelligibility is an instrument of cognition and adequate peer socialization, and language impairment in school-aged children with 22q11DS might be an indicator of increased risk for later psychotic symptoms, patients with 22q11.2 microdeletion should be included in a program of early stimulation of speech–language development immediately after diagnosis is established.",
publisher = "MDPI",
journal = "Children, Children",
title = "Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion",
number = "4",
pages = "489",
volume = "11",
doi = "10.3390/children11040489"
}

Rakonjac, M., Cuturilo, G., Kovačević-Grujičić, N., Simeunović, I., Kostić, J., Stevanović, M.,& Drakulić, D.. (2024). Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion. in Children
MDPI., 11(4), 489.
https://doi.org/10.3390/children11040489

Rakonjac M, Cuturilo G, Kovačević-Grujičić N, Simeunović I, Kostić J, Stevanović M, Drakulić D. Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion. in Children. 2024;11(4):489.
doi:10.3390/children11040489 .

Rakonjac, Marijana, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Simeunović, Ivana, Kostić, Jovana, Stevanović, Milena, Drakulić, Danijela, "Speech Sounds Production, Narrative Skills, and Verbal Memory of Children with 22q11.2 Microdeletion" in Children, 11, no. 4 (2024):489,
https://doi.org/10.3390/children11040489 . .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Petrakis, Spyros
AU  - Harwood, Adrian J.
AU  - Linden, David
AU  - Lazić, Andrijana
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1325
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2361
AB  - Neurodevelopmental disorders (NDDs) are caused by alterations in early brain development. They are a group of geographically dispersed, complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. In order to build global research activity for study of NDDs, the main goals of the Twinning project STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans. This will be achieved by twinning IMGGE with three top-class research institutions in Europe (Cardiff University, University of Maastricht and Centre for Research and Technology Hellas) with an exceptional expertise in the stem cells based research of NDDs, -OMICS technologies, bioinformatics data analysis and drug testing and through staff exchanges, training, and organization of summer schools, Industry Open Days, symposia and workshops.
C3  - Hemijska industrija (Chemical Industry)
T1  - STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region
EP  - 78
IS  - 1S
SP  - 78
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2361
ER  - 

@conference{
author = "Drakulić, Danijela and Petrakis, Spyros and Harwood, Adrian J. and Linden, David and Lazić, Andrijana and Kovačević-Grujičić, Nataša and Stevanović, Milena",
year = "2024",
abstract = "Neurodevelopmental disorders (NDDs) are caused by alterations in early brain development. They are a group of geographically dispersed, complex and heterogeneous disorders that give rise to the psychiatric conditions such as autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. In order to build global research activity for study of NDDs, the main goals of the Twinning project STREAMLINE are to enhanced strategic networking and reinforce research and innovation potential of the Institute of Molecular Genetics and Genetic Engineering, University of Belgrade (IMGGE) in order to develop IMGGE as a high capacity hub for research of NDDs in the Western Balkans. This will be achieved by twinning IMGGE with three top-class research institutions in Europe (Cardiff University, University of Maastricht and Centre for Research and Technology Hellas) with an exceptional expertise in the stem cells based research of NDDs, -OMICS technologies, bioinformatics data analysis and drug testing and through staff exchanges, training, and organization of summer schools, Industry Open Days, symposia and workshops.",
journal = "Hemijska industrija (Chemical Industry)",
title = "STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region",
pages = "78-78",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2361"
}

Drakulić, D., Petrakis, S., Harwood, A. J., Linden, D., Lazić, A., Kovačević-Grujičić, N.,& Stevanović, M.. (2024). STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region. in Hemijska industrija (Chemical Industry), 78(1S), 78-78.
https://hdl.handle.net/21.15107/rcub_imagine_2361

Drakulić D, Petrakis S, Harwood AJ, Linden D, Lazić A, Kovačević-Grujičić N, Stevanović M. STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region. in Hemijska industrija (Chemical Industry). 2024;78(1S):78-78.
https://hdl.handle.net/21.15107/rcub_imagine_2361 .

Drakulić, Danijela, Petrakis, Spyros, Harwood, Adrian J., Linden, David, Lazić, Andrijana, Kovačević-Grujičić, Nataša, Stevanović, Milena, "STREAMLINE HUB: a high capacity hub for research of neurodevelopmental disorders in the Western Balkan region" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):78-78,
https://hdl.handle.net/21.15107/rcub_imagine_2361 .

TY  - CONF
AU  - Bojić, Luka
AU  - Pejić, Jelena
AU  - Stojkovska, Jasmina
AU  - Stevanović, Milena
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Milivojević, Milena
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1262
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2366
AB  - Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.
C3  - Hemijska industrija (Chemical Industry)
T1  - Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems
EP  - 20
IS  - 1S
SP  - 20
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2366
ER  - 

@conference{
author = "Bojić, Luka and Pejić, Jelena and Stojkovska, Jasmina and Stevanović, Milena and Medić, Aleksandra and Petrović, Isidora and Milivojević, Milena",
year = "2024",
abstract = "Osteosarcoma (OS) is a highly aggressive primary malignant bone tumor that most commonly affects children, adolescents, and young adults. The standard treatment for OS consists of surgical resection and chemotherapy, whereas radiation therapy is recommended for the unresectable tumor. Due to its easy metastasis and recurrence, the 5-year overall survival rate is only 66.5 %. Thus, there is a critical need to recognize the molecular mechanisms underlying OS development and pathogenesis. Traditionally, two-dimensional (2D) cells are widely used in cancer biology and pre-clinical studies. However, 2D models are unable to mimic cell–cell and cell-extracellular matrix interactions which are crucial for adequate cellular function. Three-dimensional (3D) model systems are able to recapitulate key features of human cancer and are recognized as a promising platform for fundamental and translational research. In the present work, we established an osteosarcoma 3D model based on alginate microbeads and studied the effect of combined treatment with doxorubicin (Doxo), widely used chemotherapeutic, and quercetin (Quer), a plant pigment with anticancer properties, on OS model systems.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems",
pages = "20-20",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2366"
}

Bojić, L., Pejić, J., Stojkovska, J., Stevanović, M., Medić, A., Petrović, I.,& Milivojević, M.. (2024). Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry), 78(1S), 20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366

Bojić L, Pejić J, Stojkovska J, Stevanović M, Medić A, Petrović I, Milivojević M. Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems. in Hemijska industrija (Chemical Industry). 2024;78(1S):20-20.
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

Bojić, Luka, Pejić, Jelena, Stojkovska, Jasmina, Stevanović, Milena, Medić, Aleksandra, Petrović, Isidora, Milivojević, Milena, "Doxorubicin and quercetin combined effect on SAOS-2 cells grown in 2D and 3D model systems" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):20-20,
https://hdl.handle.net/21.15107/rcub_imagine_2366 .

TY  - CONF
AU  - Petrović, Jelena
AU  - Pańczyszyn, Elżbieta
AU  - Corazzari, Marco
AU  - Banićević, Ivana
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Stevanović, Milena
AU  - Dragoj, Miodrag
AU  - Pešić, Milica
AU  - Janković, Radmila
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1264
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2364
AB  - The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.
C3  - Hemijska industrija (Chemical Industry)
T1  - Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model
EP  - 21
IS  - 1S
SP  - 21
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2364
ER  - 

@conference{
author = "Petrović, Jelena and Pańczyszyn, Elżbieta and Corazzari, Marco and Banićević, Ivana and Milivojević, Milena and Bojić, Luka and Stevanović, Milena and Dragoj, Miodrag and Pešić, Milica and Janković, Radmila and Obradović, Bojana and Stojkovska, Jasmina",
year = "2024",
abstract = "The slow advance in anticancer drug development can be attributed to the limitations of conventional models, predominantly monolayer cell (2D) cultures and animal models, which inadequately recapitulate the complex nature of human malignant tumors. Three-dimensional (3D) in vitro models are invaluable tools in drug screening; however, creating a universal model for all cancer types poses challenges due to the diverse nature of cancers. The aim of this work was to develop a single, versatile model using alginate microfibers to accommodate cultivation of various cancer cells.",
journal = "Hemijska industrija (Chemical Industry)",
title = "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model",
pages = "21-21",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2364"
}

Petrović, J., Pańczyszyn, E., Corazzari, M., Banićević, I., Milivojević, M., Bojić, L., Stevanović, M., Dragoj, M., Pešić, M., Janković, R., Obradović, B.,& Stojkovska, J.. (2024). Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry), 78(1S), 21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364

Petrović J, Pańczyszyn E, Corazzari M, Banićević I, Milivojević M, Bojić L, Stevanović M, Dragoj M, Pešić M, Janković R, Obradović B, Stojkovska J. Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model. in Hemijska industrija (Chemical Industry). 2024;78(1S):21-21.
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

Petrović, Jelena, Pańczyszyn, Elżbieta, Corazzari, Marco, Banićević, Ivana, Milivojević, Milena, Bojić, Luka, Stevanović, Milena, Dragoj, Miodrag, Pešić, Milica, Janković, Radmila, Obradović, Bojana, Stojkovska, Jasmina, "Adaptable alginate-based microfibers for 3D in vitro cultures of cancer cells: an anticancer drug testing model" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):21-21,
https://hdl.handle.net/21.15107/rcub_imagine_2364 .

TY  - CONF
AU  - Banićević, Ivana
AU  - Milošević, Mia
AU  - Petrović, Jelena
AU  - Menshikh, Ksenia
AU  - Milivojević, Milena
AU  - Stevanović, Milena
AU  - Janković, Radmila
AU  - Cochis, Andrea
AU  - Bella, Elena Della
AU  - Stoddart, Martin
AU  - Rimondini, Lia
AU  - Stojkovska, Jasmina
AU  - Obradović, Bojana
PY  - 2024
UR  - https://www.ache-pub.org.rs/index.php/HemInd/article/view/1261
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2363
AB  - Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.
C3  - Hemijska industrija (Chemical Industry)
T1  - A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research
EP  - 19
IS  - 1S
SP  - 19
VL  - 78
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2363
ER  - 

@conference{
author = "Banićević, Ivana and Milošević, Mia and Petrović, Jelena and Menshikh, Ksenia and Milivojević, Milena and Stevanović, Milena and Janković, Radmila and Cochis, Andrea and Bella, Elena Della and Stoddart, Martin and Rimondini, Lia and Stojkovska, Jasmina and Obradović, Bojana",
year = "2024",
abstract = "Comprehensive research, particularly in evaluating drug efficacy, still heavily relies on the results obtained by the utilization of cell monolayers and animals. However, the inherent limitations of these models such as their physiological disparities from humans pose significant obstacles to acquiring reliable results thus impeding further scientific progression. To address this challenge, 3D in vitro cell culture models emerged as physiologically relevant models having the potential to enhance research and drug discovery. Our study aimed to develop a 3D in vitro cell culture model based on bone-like scaffolds in conjunction with a perfusion bioreactor (“3D Perfuse”, Innovation Center FTM, Belgrade, Serbia) for studying both physiological and pathological (i.e. tumors) bone conditions.",
journal = "Hemijska industrija (Chemical Industry)",
title = "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research",
pages = "19-19",
number = "1S",
volume = "78",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2363"
}

Banićević, I., Milošević, M., Petrović, J., Menshikh, K., Milivojević, M., Stevanović, M., Janković, R., Cochis, A., Bella, E. D., Stoddart, M., Rimondini, L., Stojkovska, J.,& Obradović, B.. (2024). A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry), 78(1S), 19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363

Banićević I, Milošević M, Petrović J, Menshikh K, Milivojević M, Stevanović M, Janković R, Cochis A, Bella ED, Stoddart M, Rimondini L, Stojkovska J, Obradović B. A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research. in Hemijska industrija (Chemical Industry). 2024;78(1S):19-19.
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

Banićević, Ivana, Milošević, Mia, Petrović, Jelena, Menshikh, Ksenia, Milivojević, Milena, Stevanović, Milena, Janković, Radmila, Cochis, Andrea, Bella, Elena Della, Stoddart, Martin, Rimondini, Lia, Stojkovska, Jasmina, Obradović, Bojana, "A 3D in vitro cell culture model based on perfused bone-like scaffolds for healthy and pathological bone research" in Hemijska industrija (Chemical Industry), 78, no. 1S (2024):19-19,
https://hdl.handle.net/21.15107/rcub_imagine_2363 .

TY  - CONF
AU  - Pejić, Jelena
AU  - Mojsin, Marija
AU  - Stojkovska, Jasmina
AU  - Medić, Aleksandra
AU  - Petrović, Isidora
AU  - Stevanović, Milena
AU  - Obradović, Bojana
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2151
AB  - Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds
EP  - 113
SP  - 113
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2151
ER  - 

@conference{
author = "Pejić, Jelena and Mojsin, Marija and Stojkovska, Jasmina and Medić, Aleksandra and Petrović, Isidora and Stevanović, Milena and Obradović, Bojana and Milivojević, Milena",
year = "2023",
abstract = "Introduction: The NT2/D1 embryonal carcinoma cell line represents a well-established in vitro model of
human neurogenesis. It’s widely used for studying neurodevelopmental processes, neurotoxicity, and
neurodegenerative disorders. The utilization of alginate fibers as a 3D cell culture system offers a biocompatible and structurally supportive environment for neural differentiation and maturation of cells,
making it a suitable tool for investigating neurodevelopmental processes.
Methods: In thisstudy, we evaluated the alginate microfibers as a 3D modelsystem for in vitro neural differentiation of NT2/D1 cells.We described the immobilization of NT2/D1 cellsin alginate microfibers and
the effect of propagation in this 3D model on morphological features, viability, and proliferation of immobilized cells. We also assessed the RA-induced initiation of neural differentiation of NT2/D1 cellsin alginate microfibers by comparison with the initiation of neural differentiation in adherent 2D cell culture.
Results: Our results showed that immobilized NT2/D1 acquired morphological features characteristic
of cells propagated in 3D model systems and retain viability, proliferative capacity, and ability to attach
to adherent surfaces. In addition, immobilized NT2/D1 cells preserved neural differentiation capacity.
Upon RA induction we detected a marked decrease in the expression of specific pluripotency-maintaining markers, SOX2, OCT4, and NANOG. Consecutively, the expression of early neural markers, SOX3,
PAX6, and miR219 was significantly increased.
Conclusion: Neural differentiation of NT2/D1 cellsimmobilized within alginate fibersrepresents a highly
promising 3D modelsystem forstudying human neurogenesis and offers a valuable platform forscreening the effect of drugs and bioactive compounds on human neural differentiation.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds",
pages = "113-113",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2151"
}

Pejić, J., Mojsin, M., Stojkovska, J., Medić, A., Petrović, I., Stevanović, M., Obradović, B.,& Milivojević, M.. (2023). Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151

Pejić J, Mojsin M, Stojkovska J, Medić A, Petrović I, Stevanović M, Obradović B, Milivojević M. Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:113-113.
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

Pejić, Jelena, Mojsin, Marija, Stojkovska, Jasmina, Medić, Aleksandra, Petrović, Isidora, Stevanović, Milena, Obradović, Bojana, Milivojević, Milena, "Immobilized NT2/D1 cells in alginate fibers: a promising 3D model system for investigating human neurogenesis and screening the effect of drugs and bioactive compounds" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):113-113,
https://hdl.handle.net/21.15107/rcub_imagine_2151 .

TY  - JOUR
AU  - Kloska, Anna
AU  - Giełczyk, Agata
AU  - Grzybowski, Tomasz
AU  - Płoski, Rafał
AU  - Kloska, Sylwester M.
AU  - Marciniak, Tomasz
AU  - Pałczyński, Krzysztof
AU  - Rogalla-Ładniak, Urszula
AU  - Malyarchuk, Boris A.
AU  - Derenko, Miroslava V.
AU  - Kovačević-Grujičić, Nataša
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
AU  - Davidović, Slobodan
AU  - Spólnicka, Magdalena
AU  - Zubańska, Magdalena
AU  - Woźniak, Marcin
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/20/15095
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2171
AB  - Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.
T2  - International Journal of Molecular Sciences
T1  - A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe
IS  - 20
SP  - 15095
VL  - 24
DO  - 10.3390/ijms242015095
ER  - 

@article{
author = "Kloska, Anna and Giełczyk, Agata and Grzybowski, Tomasz and Płoski, Rafał and Kloska, Sylwester M. and Marciniak, Tomasz and Pałczyński, Krzysztof and Rogalla-Ładniak, Urszula and Malyarchuk, Boris A. and Derenko, Miroslava V. and Kovačević-Grujičić, Nataša and Stevanović, Milena and Drakulić, Danijela and Davidović, Slobodan and Spólnicka, Magdalena and Zubańska, Magdalena and Woźniak, Marcin",
year = "2023",
abstract = "Data obtained with the use of massive parallel sequencing (MPS) can be valuable in population genetics studies. In particular, such data harbor the potential for distinguishing samples from different populations, especially from those coming from adjacent populations of common origin. Machine learning (ML) techniques seem to be especially well suited for analyzing large datasets obtained using MPS. The Slavic populations constitute about a third of the population of Europe and inhabit a large area of the continent, while being relatively closely related in population genetics terms. In this proof-of-concept study, various ML techniques were used to classify DNA samples from Slavic and non-Slavic individuals. The primary objective of this study was to empirically evaluate the feasibility of discerning the genetic provenance of individuals of Slavic descent who exhibit genetic similarity, with the overarching goal of categorizing DNA specimens derived from diverse Slavic population representatives. Raw sequencing data were pre-processed, to obtain a 1200 character-long binary vector. A total of three classifiers were used—Random Forest, Support Vector Machine (SVM), and XGBoost. The most-promising results were obtained using SVM with a linear kernel, with 99.9% accuracy and F1-scores of 0.9846–1.000 for all classes.",
journal = "International Journal of Molecular Sciences",
title = "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe",
number = "20",
pages = "15095",
volume = "24",
doi = "10.3390/ijms242015095"
}

Kloska, A., Giełczyk, A., Grzybowski, T., Płoski, R., Kloska, S. M., Marciniak, T., Pałczyński, K., Rogalla-Ładniak, U., Malyarchuk, B. A., Derenko, M. V., Kovačević-Grujičić, N., Stevanović, M., Drakulić, D., Davidović, S., Spólnicka, M., Zubańska, M.,& Woźniak, M.. (2023). A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences, 24(20), 15095.
https://doi.org/10.3390/ijms242015095

Kloska A, Giełczyk A, Grzybowski T, Płoski R, Kloska SM, Marciniak T, Pałczyński K, Rogalla-Ładniak U, Malyarchuk BA, Derenko MV, Kovačević-Grujičić N, Stevanović M, Drakulić D, Davidović S, Spólnicka M, Zubańska M, Woźniak M. A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe. in International Journal of Molecular Sciences. 2023;24(20):15095.
doi:10.3390/ijms242015095 .

Kloska, Anna, Giełczyk, Agata, Grzybowski, Tomasz, Płoski, Rafał, Kloska, Sylwester M., Marciniak, Tomasz, Pałczyński, Krzysztof, Rogalla-Ładniak, Urszula, Malyarchuk, Boris A., Derenko, Miroslava V., Kovačević-Grujičić, Nataša, Stevanović, Milena, Drakulić, Danijela, Davidović, Slobodan, Spólnicka, Magdalena, Zubańska, Magdalena, Woźniak, Marcin, "A Machine-Learning-Based Approach to Prediction of Biogeographic Ancestry within Europe" in International Journal of Molecular Sciences, 24, no. 20 (2023):15095,
https://doi.org/10.3390/ijms242015095 . .

TY  - CHAP
AU  - Davidović, Slobodan
AU  - Aleksić, Jelena
AU  - Stevanović, Milena
AU  - Kovačević Grujičić, Nataša
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2245
AB  - Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji
Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima
zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne
istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje
visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom
vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog
za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju
unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija
deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje
pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske
i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,
karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i
one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga,
srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama
kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala
da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom
kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo
pre Velike seobe naroda.
AB  - The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for studying
the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including complete
mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data confirmed
that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest percentage
of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phylogenetic
and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lineages, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and
Africa (L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and
H6a2b. In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and
West-Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies
of mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian
population may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-
Slavic populations that inhabited the Balkan Peninsula before the Great Migration.
PB  - Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo
T2  - Trendovi u molekularnoj Biologiji
T1  - Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije
T1  - Mitochondrial gene pool variability of the residents of the Republic of Serbia
EP  - 36
IS  - 3
SP  - 18
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2245
ER  - 

@inbook{
author = "Davidović, Slobodan and Aleksić, Jelena and Stevanović, Milena and Kovačević Grujičić, Nataša",
year = "2023",
abstract = "Mitohondrijska DNK (mtDNK) se odlikuje nizom osobina koje je čine pogodnom za istraživanja evolutivne
istorije ljudskih populacija koja se zasniva na molekularnim markerima ženske linije nasleđivanja. Tokom
poslednje decenije publikovano je više naučnih radova u kojima je analizirana varijabilnost mtDNK u populaciji
Srbije primenom markera različite rezolucije uključujući i kompletne genome. U skladu sa očekivanjima
zasnovanim na istorijskim, arheološkim i drugim izvorima koji govore u prilog veoma kompleksne
istorije populacija na Balkanskom poluostrvu, mtDNK podaci su potvrdili da se srpska populacija odlikuje
visokim nivoom raznovrsnosti mtDNK koji je posledica izuzetno složene dinamike ove populacije tokom
vremena. Današnji mtDNK profil populacije Srbije ne odstupa od matrilinealnog profila karakterističnog
za druge evropske populacije, a genetičke distance pokazuju da ova populacija zauzima centralnu poziciju
unutar grupe južnoslovenskih populacija koje se odlikuju visokom heterogenošću. Srpska populacija
deli najveći procenat mtDNK haplotipova sa geografski bliskim populacijama Balkanskog poluostrva koje
pripadaju južnoslovenskoj grupi, gde su uočeni i potencijalno privatni haplotipovi. Na osnovu filogenetske
i filogeografske analize kompletnih mitogenoma u srpskoj populaciji detektovane su retke mtDNK linije,
karakteristične za druge regione, poput Bliskog istoka (N1b, HV2), istočne Azije (D4) i Afrike (L2a1), kao i
one koje su potencijalno specifične za Balkansko poluostrvo, poput K1a13a1, U4c1b1 i H6a2b. Pored toga,
srpska populacija deli određeni broj mtDNK podhaplogrupa sa istočno- i zapadnoslovenskim populacijama
kao i sa germanskim populacijama severne i srednje Evrope. Istraživanja varijabilnosti mtDNK su pokazala
da se izuzetno velika raznovrsnost mtDNK savremene populacije Srbije može objasniti genetičkim doprinosom
kako slovenskih i germanskih, tako i pre-slovenskih populacija koje su naseljavale Balkansko poluostrvo
pre Velike seobe naroda., The mitochondrial DNA (mtDNA) is characterized by a number of features that make it suitable for studying
the evolutionary history of human populations based on molecular markers with the female-specific
line of inheritance. During the last decade, several scientific papers were published in which the mtDNA
variability in the population of Serbia was analyzed using markers of different resolution including complete
mitogenomes. In accordance with expectations based on historical, archaeological and other sources
that speak in favor of a very complex history of populations on the Balkan Peninsula, mtDNA data confirmed
that Serbian population is characterized by a high level of mtDNA diversity, which is a consequence
of the exceptionally complex dynamics of this population over time. Today’s mtDNA profile of the Serbian
population does not differ from the matrilineal landscape characteristic of other European populations,
and according to genetic distances, this population occupies a central position within the group of South-
Slavic populations characterized by high heterogeneity. The Serbian population shares the highest percentage
of mtDNA haplotypes with the geographically close populations of the Balkan Peninsula
belonging to the South-Slavic group, where potentially private haplotypes were also observed. Phylogenetic
and phylogeographic analysis of complete mitogenomes in the Serbian population revealed rare
mtDNA lineages, characteristic of other regions, such as the Middle East (N1b, HV2), East Asia (D4) and
Africa (L2a1), as well as those that are potentially specific for Balkan Peninsula, like K1a13a1, U4c1b1 and
H6a2b. In addition, Serbian population shares a certain number of mtDNA subhaplogroups with East- and
West-Slavic populations as well as with the Germanic populations of Northern and Central Europe. Studies
of mtDNA variability have shown that the exceptionally high mtDNA diversity in contemporary Serbian
population may be associated with the genetic contribution of both Slavic and Germanic, as well as pre-
Slavic populations that inhabited the Balkan Peninsula before the Great Migration.",
publisher = "Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo",
journal = "Trendovi u molekularnoj Biologiji",
booktitle = "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije, Mitochondrial gene pool variability of the residents of the Republic of Serbia",
pages = "36-18",
number = "3",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2245"
}

Davidović, S., Aleksić, J., Stevanović, M.,& Kovačević Grujičić, N.. (2023). Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trendovi u molekularnoj Biologiji
Beograd : Institut za molekularnu genetiku i genetičko inženjerstvo.(3), 18-36.
https://hdl.handle.net/21.15107/rcub_imagine_2245

Davidović S, Aleksić J, Stevanović M, Kovačević Grujičić N. Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije. in Trendovi u molekularnoj Biologiji. 2023;(3):18-36.
https://hdl.handle.net/21.15107/rcub_imagine_2245 .

Davidović, Slobodan, Aleksić, Jelena, Stevanović, Milena, Kovačević Grujičić, Nataša, "Varijabilnost mitohondrijskog genskog pula stanovnika Republike Srbije" in Trendovi u molekularnoj Biologiji, no. 3 (2023):18-36,
https://hdl.handle.net/21.15107/rcub_imagine_2245 .

TY  - CONF
AU  - Balint, Vanda
AU  - Stanisavljević-Ninković, Danijela
AU  - Lazić, Stefan
AU  - Kovačević-Grujičić, Nataša
AU  - Perić, Mina
AU  - Pejić, Jelena
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2185
AB  - Astrocytes are the main homeostatic cells in the brain with important roles both in
physiological and pathological conditions. They have a unique ability to become
reactivated in response to different types of brain pathologies, which serves as a
compensatory response that modulates tissue damage and recovery. Also, senescent
astrocytes have profound implications in age-related neurodegenerative disorders. The
molecular mechanisms underlying astrocyte reactivation and senescence are still not
well understood. To investigate the roles of SOX2 and SOX9 transcription factors and
miR-21 in these phenotypic alternations of astroglia, astrocytes derived from NT2/D1
cell line (NT2/A) were used as a model system. Western blot analyses showed that the
expression of both SOX2 and SOX9 decreases during the maturation of NT2/A and
they are re-expressed upon in vitro induced injury. Further modulation of the SOX2
and SOX9 expression will reveal their roles in the regulation of astrocytes
reactivation. Down-regulation of mir-21 in both immature and mature NT2/A by
using the antisense technology, induced the decline in cell proliferation revealed by
Ki67 proliferation marker. Also the premature cellular senescence was induced as
indicated by increase in SA-ß-gal activity and the expression of p21 and p53.
Additionally, in silico analysis predicted many of the genes, previously shown to be
upregulated in senescent astrocytes, as miR-21 targets.
Clarifying the roles of SOX genes and miRNAs in astrocyte reactivation and
senescence would contribute to better understanding of the functions of these cells at
the molecular level, which holds promise for development of new therapeutic
strategies.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells
EP  - 99
SP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2185
ER  - 

@conference{
author = "Balint, Vanda and Stanisavljević-Ninković, Danijela and Lazić, Stefan and Kovačević-Grujičić, Nataša and Perić, Mina and Pejić, Jelena and Mojsin, Marija and Stevanović, Milena and Lazić, Andrijana",
year = "2023",
abstract = "Astrocytes are the main homeostatic cells in the brain with important roles both in
physiological and pathological conditions. They have a unique ability to become
reactivated in response to different types of brain pathologies, which serves as a
compensatory response that modulates tissue damage and recovery. Also, senescent
astrocytes have profound implications in age-related neurodegenerative disorders. The
molecular mechanisms underlying astrocyte reactivation and senescence are still not
well understood. To investigate the roles of SOX2 and SOX9 transcription factors and
miR-21 in these phenotypic alternations of astroglia, astrocytes derived from NT2/D1
cell line (NT2/A) were used as a model system. Western blot analyses showed that the
expression of both SOX2 and SOX9 decreases during the maturation of NT2/A and
they are re-expressed upon in vitro induced injury. Further modulation of the SOX2
and SOX9 expression will reveal their roles in the regulation of astrocytes
reactivation. Down-regulation of mir-21 in both immature and mature NT2/A by
using the antisense technology, induced the decline in cell proliferation revealed by
Ki67 proliferation marker. Also the premature cellular senescence was induced as
indicated by increase in SA-ß-gal activity and the expression of p21 and p53.
Additionally, in silico analysis predicted many of the genes, previously shown to be
upregulated in senescent astrocytes, as miR-21 targets.
Clarifying the roles of SOX genes and miRNAs in astrocyte reactivation and
senescence would contribute to better understanding of the functions of these cells at
the molecular level, which holds promise for development of new therapeutic
strategies.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells",
pages = "99-99",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2185"
}

Balint, V., Stanisavljević-Ninković, D., Lazić, S., Kovačević-Grujičić, N., Perić, M., Pejić, J., Mojsin, M., Stevanović, M.,& Lazić, A.. (2023). The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2185

Balint V, Stanisavljević-Ninković D, Lazić S, Kovačević-Grujičić N, Perić M, Pejić J, Mojsin M, Stevanović M, Lazić A. The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells. in 8th Congress of the Serbian Neuroscience Society. 2023;:99-99.
https://hdl.handle.net/21.15107/rcub_imagine_2185 .

Balint, Vanda, Stanisavljević-Ninković, Danijela, Lazić, Stefan, Kovačević-Grujičić, Nataša, Perić, Mina, Pejić, Jelena, Mojsin, Marija, Stevanović, Milena, Lazić, Andrijana, "The role of specific SOX genes and microRNAs in reactivation and senescence of human astrocytes derived from pluripotent NT2/D1 cells" in 8th Congress of the Serbian Neuroscience Society (2023):99-99,
https://hdl.handle.net/21.15107/rcub_imagine_2185 .

TY  - CONF
AU  - Bojić, Luka
AU  - Schwirtlich, Marija
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Balint, Vanda
AU  - Stevanović, Milena
AU  - Milivojević, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2133
AB  - Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro
EP  - 143
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2133
ER  - 

@conference{
author = "Bojić, Luka and Schwirtlich, Marija and Lazić, Stefan and Stanisavljević Ninković, Danijela and Balint, Vanda and Stevanović, Milena and Milivojević, Milena",
year = "2023",
abstract = "Introduction: Prolonged exposure to sunlight, has a harmful effect on skin cells encompassing reduced
viability, morphological changes, and altered gene expression. The two most prevalent types ofskin cancer,squamous cell carcinoma (cSCC) and basal cell carcinoma (BCC), arise from malignant transformation
of keratinocytes. UV radiation, among other factors, serves as the primary cause of these tumors. Previous data hasshown that changesin different SOX genes expression in these cancer types correlates with
disease progression, suggesting their role as oncogenes/tumor suppressors. The presented work is focused on examining the impact of UVB radiation on the expression of SOX2 and SOX9 genesin HaCaT cells
derived from human keratinocytes.
Methods: Using a custom-made UV solarsimulator for the irradiation of HaCaT cells with 150 mJ/cm2 or
300 mJ/cm2
, we analyzed SOX2 and SOX9 gene expression. In order to determine the protective effects
of quercetin, anti-inflammatory bioflavonoid, we treated irradiated HaCaT with quercetin, and analyzed
SOX gene expression.
Results: Our resultsindicate that UVB radiation induces a dose dependent decrease of SOX2 expression
while expression of SOX9 was increased at the dose of 150 mJ/cm2 in HaCaT. Treatment of cells with
quercetin increased the expression of both SOX2 and SOX9 genesin HaCaT cellsfollowing UVB radiation
at both doses compared to irradiated cells.
Conclusions: Further research is needed to understand the molecular mechanisms and significance of
SOX2 and SOX9 in UVB-induced cellular responses, in the context of nonmelanoma cancers with potential implications for targeted therapeutic strategies for nonmelanoma cancers",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro",
pages = "143-143",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2133"
}

Bojić, L., Schwirtlich, M., Lazić, S., Stanisavljević Ninković, D., Balint, V., Stevanović, M.,& Milivojević, M.. (2023). The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2133

Bojić L, Schwirtlich M, Lazić S, Stanisavljević Ninković D, Balint V, Stevanović M, Milivojević M. The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:143-143.
https://hdl.handle.net/21.15107/rcub_imagine_2133 .

Bojić, Luka, Schwirtlich, Marija, Lazić, Stefan, Stanisavljević Ninković, Danijela, Balint, Vanda, Stevanović, Milena, Milivojević, Milena, "The effect of UVB radiation onthe expression of SOX2 and SOX9 genes in human keratinocytes in vitro" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):143-143,
https://hdl.handle.net/21.15107/rcub_imagine_2133 .

TY  - CONF
AU  - Lazić, Stefan
AU  - Stanisavljević Ninković, Danijela
AU  - Petrović, Isidora
AU  - Aleksandra, Medić
AU  - Milivojević, Milena
AU  - Bojić, Luka
AU  - Erceg, Slaven
AU  - Stevanović, Milena
AU  - Švirtlih, Marija
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2287
AB  - Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21
EP  - 96
SP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2287
ER  - 

@conference{
author = "Lazić, Stefan and Stanisavljević Ninković, Danijela and Petrović, Isidora and Aleksandra, Medić and Milivojević, Milena and Bojić, Luka and Erceg, Slaven and Stevanović, Milena and Švirtlih, Marija",
year = "2023",
abstract = "Brain trauma leads to the induction of neural stem cell proliferation and the migration
of young neurons to injured areas. However, these neurons are insufficient to fully
restore neuronal function due to the limited potential of adult neurogenesis. This study
aimed to investigate the effect of hypoxia, a condition that underlines a wide spectrum
of brain pathologies, on pluripotency and the capacity of stem cells to differentiate
into neural progenitors. We analyzed the expression of SOX genes and microRNAs as
they control a variety of cellular processes during neuronal differentiation, including
cell proliferation and cell fate determination. In vitro neuronal differentiation of
human embryonal carcinoma cell line NT2/D1 and induced pluripotent stem cells
were used as a model system of adult neurogenesis. Cobalt chloride was used to
induce hypoxia.
The results of the analysis showed that, following hypoxia, the efficiency of neuronal
induction was significantly decreased, that coincident with decline in mRNA
expression levels of SOXB and SOXC genes. In contrast to that, the expression level of
miR-21 was significantly increased.
Our findings advance the study of SOX TFs, miR-21, and their possible interplay in
ischemia-related pathologies, establishing them as prospective biomarkers and
possible targets for future diagnostic and therapeutic approaches.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21",
pages = "96-96",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2287"
}

Lazić, S., Stanisavljević Ninković, D., Petrović, I., Aleksandra, M., Milivojević, M., Bojić, L., Erceg, S., Stevanović, M.,& Švirtlih, M.. (2023). Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287

Lazić S, Stanisavljević Ninković D, Petrović I, Aleksandra M, Milivojević M, Bojić L, Erceg S, Stevanović M, Švirtlih M. Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21. in 8th Congress of the Serbian Neuroscience Society. 2023;:96-96.
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

Lazić, Stefan, Stanisavljević Ninković, Danijela, Petrović, Isidora, Aleksandra, Medić, Milivojević, Milena, Bojić, Luka, Erceg, Slaven, Stevanović, Milena, Švirtlih, Marija, "Hypoxia preconditioning reduces the differentiation potential of human pluripotent stem cells and alters the expression of SOX genes and miR-21" in 8th Congress of the Serbian Neuroscience Society (2023):96-96,
https://hdl.handle.net/21.15107/rcub_imagine_2287 .

TY  - JOUR
AU  - Marković, Zoran
AU  - Mišović, Aleksandra
AU  - Zmejkoski, Danica
AU  - Zdravković, Nemanja
AU  - Kovač, Janez
AU  - Bajuk-Bogdanović, Danica
AU  - Milivojević, Dušan
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Pavlović, Vladimir
AU  - Todorović Marković, Biljana
PY  - 2023
UR  - https://www.mdpi.com/2079-6382/12/5/919
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1892
AB  - Nowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomaterials family, can potentially be used as a highly promising visible-light-triggered antibacterial agent. In this work, the results of antibacterial and cytotoxic activities of gamma-ray-irradiated carbon quantum dots are presented. Carbon quantum dots (CQDs) were synthesized from citric acid by a pyrolysis procedure and irradiated by gamma rays at different doses (25, 50, 100 and 200 kGy). Structure, chemical composition and optical properties were investigated by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, UV-Vis spectrometry and photoluminescence. Structural analysis showed that CQDs have a spherical-like shape and dose-dependent average diameters and heights. Antibacterial tests showed that all irradiated dots had antibacterial activity but CQDs irradiated with dose of 100 kGy had antibacterial activity against all seven pathogen-reference bacterial strains. Gamma-ray-modified CQDs did not show any cytotoxicity toward human fetal-originated MRC-5 cells. Moreover, fluorescence microscopy showed excellent cellular uptake of CQDs irradiated with doses of 25 and 200 kGy into MRC-5 cells.
T2  - Antibiotics
T1  - Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria
IS  - 5
SP  - 919
VL  - 12
DO  - 10.3390/antibiotics12050919
ER  - 

@article{
author = "Marković, Zoran and Mišović, Aleksandra and Zmejkoski, Danica and Zdravković, Nemanja and Kovač, Janez and Bajuk-Bogdanović, Danica and Milivojević, Dušan and Mojsin, Marija and Stevanović, Milena and Pavlović, Vladimir and Todorović Marković, Biljana",
year = "2023",
abstract = "Nowadays, it is a great challenge to develop new medicines for treating various infectious diseases. The treatment of these diseases is of utmost interest to further prevent the development of multi-drug resistance in different pathogens. Carbon quantum dots, as a new member of the carbon nanomaterials family, can potentially be used as a highly promising visible-light-triggered antibacterial agent. In this work, the results of antibacterial and cytotoxic activities of gamma-ray-irradiated carbon quantum dots are presented. Carbon quantum dots (CQDs) were synthesized from citric acid by a pyrolysis procedure and irradiated by gamma rays at different doses (25, 50, 100 and 200 kGy). Structure, chemical composition and optical properties were investigated by atomic force microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, Raman spectroscopy, UV-Vis spectrometry and photoluminescence. Structural analysis showed that CQDs have a spherical-like shape and dose-dependent average diameters and heights. Antibacterial tests showed that all irradiated dots had antibacterial activity but CQDs irradiated with dose of 100 kGy had antibacterial activity against all seven pathogen-reference bacterial strains. Gamma-ray-modified CQDs did not show any cytotoxicity toward human fetal-originated MRC-5 cells. Moreover, fluorescence microscopy showed excellent cellular uptake of CQDs irradiated with doses of 25 and 200 kGy into MRC-5 cells.",
journal = "Antibiotics",
title = "Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria",
number = "5",
pages = "919",
volume = "12",
doi = "10.3390/antibiotics12050919"
}

Marković, Z., Mišović, A., Zmejkoski, D., Zdravković, N., Kovač, J., Bajuk-Bogdanović, D., Milivojević, D., Mojsin, M., Stevanović, M., Pavlović, V.,& Todorović Marković, B.. (2023). Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria. in Antibiotics, 12(5), 919.
https://doi.org/10.3390/antibiotics12050919

Marković Z, Mišović A, Zmejkoski D, Zdravković N, Kovač J, Bajuk-Bogdanović D, Milivojević D, Mojsin M, Stevanović M, Pavlović V, Todorović Marković B. Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria. in Antibiotics. 2023;12(5):919.
doi:10.3390/antibiotics12050919 .

Marković, Zoran, Mišović, Aleksandra, Zmejkoski, Danica, Zdravković, Nemanja, Kovač, Janez, Bajuk-Bogdanović, Danica, Milivojević, Dušan, Mojsin, Marija, Stevanović, Milena, Pavlović, Vladimir, Todorović Marković, Biljana, "Employing Gamma-Ray-Modified Carbon Quantum Dots to Combat a Wide Range of Bacteria" in Antibiotics, 12, no. 5 (2023):919,
https://doi.org/10.3390/antibiotics12050919 . .

TY  - CONF
AU  - Simeunović, Ivana
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Kostić, Jovana
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2184
AB  - Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
intellectual disability (ID), schizophrenia, and bipolar disorder, are caused by
disruption of brain development. They affect approximately 4% of the European
population. However, molecular mechanisms underlying NDDs are still unknown.
One of the syndromes with a high risk for NDDs is 22q11.2 Deletion Syndrome
(22q11.2DS) caused by microdeletion 22q11.2. 22q11.2DS is the most common
microdeletion in humans; approximately, 25% of patients with 22q11.2DS develop
schizophrenia compared to 1% in the general population, while an ID is detected in
approximately 45% of patients and ASD in 14-50% of cases. We analyzed genomic
and clinical findings in our cohort of 35 patients with 22q11.2DS. The majority of
patients have 3 Mb deletion and nine patients have inherited 22q11.2 microdeletion
from their parents. Twenty-one different clinical presentations are revealed in the
cohort with developmental delay detected in about 50% of patients. Approximately
80% of patients have heart malformations, palatal clefts/velopharyngeal insufficiency
was detected in about 30% of them, facial dysmorphism in approximately 80% and
hypocalcemia was seen in about 20% of patients. Here we presented a cohort of
patients with 22q11.2DS which represents a good system for modeling NDDs in vitro.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia
EP  - 98
SP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2184
ER  - 

@conference{
author = "Simeunović, Ivana and Drakulić, Danijela and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Kostić, Jovana and Stevanović, Milena",
year = "2023",
abstract = "Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
intellectual disability (ID), schizophrenia, and bipolar disorder, are caused by
disruption of brain development. They affect approximately 4% of the European
population. However, molecular mechanisms underlying NDDs are still unknown.
One of the syndromes with a high risk for NDDs is 22q11.2 Deletion Syndrome
(22q11.2DS) caused by microdeletion 22q11.2. 22q11.2DS is the most common
microdeletion in humans; approximately, 25% of patients with 22q11.2DS develop
schizophrenia compared to 1% in the general population, while an ID is detected in
approximately 45% of patients and ASD in 14-50% of cases. We analyzed genomic
and clinical findings in our cohort of 35 patients with 22q11.2DS. The majority of
patients have 3 Mb deletion and nine patients have inherited 22q11.2 microdeletion
from their parents. Twenty-one different clinical presentations are revealed in the
cohort with developmental delay detected in about 50% of patients. Approximately
80% of patients have heart malformations, palatal clefts/velopharyngeal insufficiency
was detected in about 30% of them, facial dysmorphism in approximately 80% and
hypocalcemia was seen in about 20% of patients. Here we presented a cohort of
patients with 22q11.2DS which represents a good system for modeling NDDs in vitro.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia",
pages = "98-98",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2184"
}

Simeunović, I., Drakulić, D., Cuturilo, G., Kovačević-Grujičić, N., Kostić, J.,& Stevanović, M.. (2023). Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2184

Simeunović I, Drakulić D, Cuturilo G, Kovačević-Grujičić N, Kostić J, Stevanović M. Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia. in 8th Congress of the Serbian Neuroscience Society. 2023;:98-98.
https://hdl.handle.net/21.15107/rcub_imagine_2184 .

Simeunović, Ivana, Drakulić, Danijela, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Kostić, Jovana, Stevanović, Milena, "Analysis of cohort of patients with 22q11.2 deletion syndrome - a single-center experience from Serbia" in 8th Congress of the Serbian Neuroscience Society (2023):98-98,
https://hdl.handle.net/21.15107/rcub_imagine_2184 .

TY  - DATA
AU  - Marković, Zoran
AU  - Budimir, Milica
AU  - Danko, Martin
AU  - Milivojević, Dušan
AU  - Kubat, Pavel
AU  - Zmejkoski, Danica
AU  - Pavlović, Vladimir
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Todorović Marković, Biljana
PY  - 2023
UR  - https://www.beilstein-journals.org/bjnano/articles/14/17
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1894
AB  - Figure S1: (a) TEM micrograph of CQDs, (b) top view AFM image of CQDs, (c) height profile of CQDs, and (d) particle size distribution of CQDs.
T2  - Beilstein Journal of Nanotechnology
T2  - Beilstein Journal of NanotechnologyBeilstein J. Nanotechnol.
T1  - Supplementary data for the article: Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174. https://doi.org/10.3762/bjnano.14.17
EP  - 174
IS  - 1
SP  - 165
VL  - 14
DO  - 10.3762/bjnano.14.17
ER  - 

@misc{
author = "Marković, Zoran and Budimir, Milica and Danko, Martin and Milivojević, Dušan and Kubat, Pavel and Zmejkoski, Danica and Pavlović, Vladimir and Mojsin, Marija and Stevanović, Milena and Todorović Marković, Biljana",
year = "2023",
abstract = "Figure S1: (a) TEM micrograph of CQDs, (b) top view AFM image of CQDs, (c) height profile of CQDs, and (d) particle size distribution of CQDs.",
journal = "Beilstein Journal of Nanotechnology, Beilstein Journal of NanotechnologyBeilstein J. Nanotechnol.",
title = "Supplementary data for the article: Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174. https://doi.org/10.3762/bjnano.14.17",
pages = "174-165",
number = "1",
volume = "14",
doi = "10.3762/bjnano.14.17"
}

Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Supplementary data for the article: Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174. https://doi.org/10.3762/bjnano.14.17. in Beilstein Journal of Nanotechnology, 14(1), 165-174.
https://doi.org/10.3762/bjnano.14.17

Marković Z, Budimir M, Danko M, Milivojević D, Kubat P, Zmejkoski D, Pavlović V, Mojsin M, Stevanović M, Todorović Marković B. Supplementary data for the article: Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174. https://doi.org/10.3762/bjnano.14.17. in Beilstein Journal of Nanotechnology. 2023;14(1):165-174.
doi:10.3762/bjnano.14.17 .

Marković, Zoran, Budimir, Milica, Danko, Martin, Milivojević, Dušan, Kubat, Pavel, Zmejkoski, Danica, Pavlović, Vladimir, Mojsin, Marija, Stevanović, Milena, Todorović Marković, Biljana, "Supplementary data for the article: Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174. https://doi.org/10.3762/bjnano.14.17" in Beilstein Journal of Nanotechnology, 14, no. 1 (2023):165-174,
https://doi.org/10.3762/bjnano.14.17 . .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Jovanović, Ida
AU  - Krstić, Aleksandar
AU  - Milivojević, Milena
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2181
AB  - Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.
PB  - Springer Nature
C3  - European Journal of Human Genetics
T1  - Detection rate of 22q11.2 microdeletion using strict diagnostic criteria
EP  - 240
IS  - Suppl 1
SP  - 240
VL  - 31
DO  - 10.1038/s41431-023-01339-3
ER  - 

@conference{
author = "Drakulić, Danijela and Cuturilo, Goran and Jovanović, Ida and Krstić, Aleksandar and Milivojević, Milena and Stevanović, Milena",
year = "2023",
abstract = "Background/Objectives: 22q11.2 microdeletion, detected in
patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most
common microdeletion syndrome in humans. 22q11.2DS has high
risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2
microdeletion applying different recruitment criteria, revealed
detection rate ranging from zero to 34.7%. Here we analyzed the
frequency of 22q11.2 microdeletion among children having at
least two out of five major characteristics of 22q11.2DS: congenital
heart malformations (CHM), facial dysmorphism, immunological
problems, palatal clefts and hypocalcemia.
Methods: Children with clinical characteristics of 22q11.2DS
were analyzed. Fluorescence in situ hybridization and multiplex
ligation-dependent probe amplification analysis were applied for
detection of 22q11.2 microdeletion.
Results: 22q11.2 microdeletion was detected in approximately
40% of children. CHM was found in all patients with 22q11.2
microdeletion. Dysmorphic facial features were present in about
45%, immunological problems in 30%, overt cleft palate in about
4% and hypocalcemia in approximately 60% of patients with
22q11.2 microdeletion.
Conclusion: When at least two major features of 22q11.2DS are
taking into consideration higher detection rate is obtained compared
to one-feature criterion. These criteria could be considered
by centers in low-income countries.",
publisher = "Springer Nature",
journal = "European Journal of Human Genetics",
title = "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria",
pages = "240-240",
number = "Suppl 1",
volume = "31",
doi = "10.1038/s41431-023-01339-3"
}

Drakulić, D., Cuturilo, G., Jovanović, I., Krstić, A., Milivojević, M.,& Stevanović, M.. (2023). Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics
Springer Nature., 31(Suppl 1), 240-240.
https://doi.org/10.1038/s41431-023-01339-3

Drakulić D, Cuturilo G, Jovanović I, Krstić A, Milivojević M, Stevanović M. Detection rate of 22q11.2 microdeletion using strict diagnostic criteria. in European Journal of Human Genetics. 2023;31(Suppl 1):240-240.
doi:10.1038/s41431-023-01339-3 .

Drakulić, Danijela, Cuturilo, Goran, Jovanović, Ida, Krstić, Aleksandar, Milivojević, Milena, Stevanović, Milena, "Detection rate of 22q11.2 microdeletion using strict diagnostic criteria" in European Journal of Human Genetics, 31, no. Suppl 1 (2023):240-240,
https://doi.org/10.1038/s41431-023-01339-3 . .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Lazić, Andrijana
AU  - Schwirtlich, Marija
AU  - Stanisavljević Ninković, Danijela
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/1/851
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1891
AB  - The quest for eternal youth and immortality is as old as humankind. Ageing is an inevitable physiological process accompanied by many functional declines that are driving factors for age-related diseases. Stem cell exhaustion is one of the major hallmarks of ageing. The SOX transcription factors play well-known roles in self-renewal and differentiation of both embryonic and adult stem cells. As a consequence of ageing, the repertoire of adult stem cells present in various organs steadily declines, and their dysfunction/death could lead to reduced regenerative potential and development of age-related diseases. Thus, restoring the function of aged stem cells, inducing their regenerative potential, and slowing down the ageing process are critical for improving the health span and, consequently, the lifespan of humans. Reprograming factors, including SOX family members, emerge as crucial players in rejuvenation. This review focuses on the roles of SOX transcription factors in stem cell exhaustion and age-related diseases, including neurodegenerative diseases, visual deterioration, chronic obstructive pulmonary disease, osteoporosis, and age-related cancers. A better understanding of the molecular mechanisms of ageing and the roles of SOX transcription factors in this process could open new avenues for developing novel strategies that will delay ageing and prevent age-related diseases.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - The Role of SOX Transcription Factors in Ageing and Age-Related Diseases
IS  - 1
SP  - 851
VL  - 24
DO  - 10.3390/ijms24010851
ER  - 

@article{
author = "Stevanović, Milena and Lazić, Andrijana and Schwirtlich, Marija and Stanisavljević Ninković, Danijela",
year = "2023",
abstract = "The quest for eternal youth and immortality is as old as humankind. Ageing is an inevitable physiological process accompanied by many functional declines that are driving factors for age-related diseases. Stem cell exhaustion is one of the major hallmarks of ageing. The SOX transcription factors play well-known roles in self-renewal and differentiation of both embryonic and adult stem cells. As a consequence of ageing, the repertoire of adult stem cells present in various organs steadily declines, and their dysfunction/death could lead to reduced regenerative potential and development of age-related diseases. Thus, restoring the function of aged stem cells, inducing their regenerative potential, and slowing down the ageing process are critical for improving the health span and, consequently, the lifespan of humans. Reprograming factors, including SOX family members, emerge as crucial players in rejuvenation. This review focuses on the roles of SOX transcription factors in stem cell exhaustion and age-related diseases, including neurodegenerative diseases, visual deterioration, chronic obstructive pulmonary disease, osteoporosis, and age-related cancers. A better understanding of the molecular mechanisms of ageing and the roles of SOX transcription factors in this process could open new avenues for developing novel strategies that will delay ageing and prevent age-related diseases.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "The Role of SOX Transcription Factors in Ageing and Age-Related Diseases",
number = "1",
pages = "851",
volume = "24",
doi = "10.3390/ijms24010851"
}

Stevanović, M., Lazić, A., Schwirtlich, M.,& Stanisavljević Ninković, D.. (2023). The Role of SOX Transcription Factors in Ageing and Age-Related Diseases. in International Journal of Molecular Sciences, 24(1), 851.
https://doi.org/10.3390/ijms24010851

Stevanović M, Lazić A, Schwirtlich M, Stanisavljević Ninković D. The Role of SOX Transcription Factors in Ageing and Age-Related Diseases. in International Journal of Molecular Sciences. 2023;24(1):851.
doi:10.3390/ijms24010851 .

Stevanović, Milena, Lazić, Andrijana, Schwirtlich, Marija, Stanisavljević Ninković, Danijela, "The Role of SOX Transcription Factors in Ageing and Age-Related Diseases" in International Journal of Molecular Sciences, 24, no. 1 (2023):851,
https://doi.org/10.3390/ijms24010851 . .

TY  - CONF
AU  - Kostić, Jovana
AU  - Drakulić, Danijela
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Simeunović, Ivana
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2194
AB  - Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
schizophrenia, and intellectual disability, are caused by disruption of early brain
development. NDDs represent important public health challenge in modern societies
with prevalence of about 10 to 15% of all births and the tendency of increasing
worldwide. On the other side, treatments of NDDs are focused on symptoms due to
limited understanding of underlying pathophysiological mechanisms. Individuals with
the 22q11.2 duplication syndrome (22q11.2dup), caused by heterozygous 22q11.2
microduplication, have an elevated risk of developing NDDs. Literature data revealed
that ASD is detected in 14-25% of patients with 22q11.2dup while schizophrenia is
less common in these patients than in the general population, suggesting that
22q11.2dup might be protective against schizophrenia. We investigated genomic and
clinical findings in cohort of 8 patients with 22q11.2dup. The majority of patients
have 3Mb duplication. Five patients have 22q11.2 microduplication inherited from
their parents. Other CNVs or SNVs are detected in 5 out of 8 patients. Common
medical anomalies in our cohort of patients include developmental delay, facial
dysmorphism, heart malformations, anomalies of the skeletal system, and anomalies
affecting the eye. Characterization of a cohort of patients with 22q11.2dup is
important since 22q11.2dup represents a powerful model to get insights into the
molecular mechanisms underlying NDDs.
PB  - Belgrade : Serbian Neuroscience Society
C3  - 8th Congress of the Serbian Neuroscience Society
T1  - Genomic and clinical findings in patients with 22q11.2 duplication syndrome
EP  - 97
SP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2194
ER  - 

@conference{
author = "Kostić, Jovana and Drakulić, Danijela and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Simeunović, Ivana and Stevanović, Milena",
year = "2023",
abstract = "Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD),
schizophrenia, and intellectual disability, are caused by disruption of early brain
development. NDDs represent important public health challenge in modern societies
with prevalence of about 10 to 15% of all births and the tendency of increasing
worldwide. On the other side, treatments of NDDs are focused on symptoms due to
limited understanding of underlying pathophysiological mechanisms. Individuals with
the 22q11.2 duplication syndrome (22q11.2dup), caused by heterozygous 22q11.2
microduplication, have an elevated risk of developing NDDs. Literature data revealed
that ASD is detected in 14-25% of patients with 22q11.2dup while schizophrenia is
less common in these patients than in the general population, suggesting that
22q11.2dup might be protective against schizophrenia. We investigated genomic and
clinical findings in cohort of 8 patients with 22q11.2dup. The majority of patients
have 3Mb duplication. Five patients have 22q11.2 microduplication inherited from
their parents. Other CNVs or SNVs are detected in 5 out of 8 patients. Common
medical anomalies in our cohort of patients include developmental delay, facial
dysmorphism, heart malformations, anomalies of the skeletal system, and anomalies
affecting the eye. Characterization of a cohort of patients with 22q11.2dup is
important since 22q11.2dup represents a powerful model to get insights into the
molecular mechanisms underlying NDDs.",
publisher = "Belgrade : Serbian Neuroscience Society",
journal = "8th Congress of the Serbian Neuroscience Society",
title = "Genomic and clinical findings in patients with 22q11.2 duplication syndrome",
pages = "97-97",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2194"
}

Kostić, J., Drakulić, D., Cuturilo, G., Kovačević-Grujičić, N., Simeunović, I.,& Stevanović, M.. (2023). Genomic and clinical findings in patients with 22q11.2 duplication syndrome. in 8th Congress of the Serbian Neuroscience Society
Belgrade : Serbian Neuroscience Society., 97-97.
https://hdl.handle.net/21.15107/rcub_imagine_2194

Kostić J, Drakulić D, Cuturilo G, Kovačević-Grujičić N, Simeunović I, Stevanović M. Genomic and clinical findings in patients with 22q11.2 duplication syndrome. in 8th Congress of the Serbian Neuroscience Society. 2023;:97-97.
https://hdl.handle.net/21.15107/rcub_imagine_2194 .

Kostić, Jovana, Drakulić, Danijela, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Simeunović, Ivana, Stevanović, Milena, "Genomic and clinical findings in patients with 22q11.2 duplication syndrome" in 8th Congress of the Serbian Neuroscience Society (2023):97-97,
https://hdl.handle.net/21.15107/rcub_imagine_2194 .

TY  - CONF
AU  - Simeunović, Ivana
AU  - Čuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Petter, Olena
AU  - Perić, Mina
AU  - Kostić, Jovana
AU  - Harwood J., Adrian
AU  - Stevanović, Milena
AU  - Drakulić, Danijela
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2138
AB  - Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), intellectual disability (ID),schizophrenia, and bipolar disorder, are caused by the alterationsin early brain development. They affect approximately 4% of the European population and represent a high
socio-economic impact and financial burden. Treatments of NDDs are focused on symptoms since molecular mechanisms underlying NDDs are still unknown. One of the syndromes with a high risk for NDDs
is 22q11.2 Deletion Syndrome (22q11.2DS) caused by microdeletion 22q11.2. 22q11.2 microdeletion is
the most common microdeletion in humans; it is one of the strongest known risk factorsfor development
of psychiatric illness and the highest known genetic risk for schizophrenia (approximately, 25% of patients with 22q11.2DS develop schizophrenia compared to 1% in the general population).
Methods: Genomic and clinical findings in 35 patients with 22q11.2DS were analyzed and peripheral
blood mononuclear cells of patients with 22q11.2DS and healthy controls were reprogrammed.
Results: The majority of patients have 3 Mb deletion and nine of them have inherited 22q11.2 microdeletion from parents. Twenty-one different clinical presentations are revealed in the cohort with developmental delay detected in about 50% of patients. iPSCs were generated from four patients with
22q11.2 microdeletion and five healthy controls.
Conclusion: Cohort of patients with 22q11.2DS isform and iPSCs were generated which enable research
of molecular mechanisms underlying NDDs.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders
EP  - 84
SP  - 84
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2138
ER  - 

@conference{
author = "Simeunović, Ivana and Čuturilo, Goran and Kovačević-Grujičić, Nataša and Petter, Olena and Perić, Mina and Kostić, Jovana and Harwood J., Adrian and Stevanović, Milena and Drakulić, Danijela",
year = "2023",
abstract = "Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), intellectual disability (ID),schizophrenia, and bipolar disorder, are caused by the alterationsin early brain development. They affect approximately 4% of the European population and represent a high
socio-economic impact and financial burden. Treatments of NDDs are focused on symptoms since molecular mechanisms underlying NDDs are still unknown. One of the syndromes with a high risk for NDDs
is 22q11.2 Deletion Syndrome (22q11.2DS) caused by microdeletion 22q11.2. 22q11.2 microdeletion is
the most common microdeletion in humans; it is one of the strongest known risk factorsfor development
of psychiatric illness and the highest known genetic risk for schizophrenia (approximately, 25% of patients with 22q11.2DS develop schizophrenia compared to 1% in the general population).
Methods: Genomic and clinical findings in 35 patients with 22q11.2DS were analyzed and peripheral
blood mononuclear cells of patients with 22q11.2DS and healthy controls were reprogrammed.
Results: The majority of patients have 3 Mb deletion and nine of them have inherited 22q11.2 microdeletion from parents. Twenty-one different clinical presentations are revealed in the cohort with developmental delay detected in about 50% of patients. iPSCs were generated from four patients with
22q11.2 microdeletion and five healthy controls.
Conclusion: Cohort of patients with 22q11.2DS isform and iPSCs were generated which enable research
of molecular mechanisms underlying NDDs.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders",
pages = "84-84",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2138"
}

Simeunović, I., Čuturilo, G., Kovačević-Grujičić, N., Petter, O., Perić, M., Kostić, J., Harwood J., A., Stevanović, M.,& Drakulić, D.. (2023). Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 84-84.
https://hdl.handle.net/21.15107/rcub_imagine_2138

Simeunović I, Čuturilo G, Kovačević-Grujičić N, Petter O, Perić M, Kostić J, Harwood J. A, Stevanović M, Drakulić D. Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:84-84.
https://hdl.handle.net/21.15107/rcub_imagine_2138 .

Simeunović, Ivana, Čuturilo, Goran, Kovačević-Grujičić, Nataša, Petter, Olena, Perić, Mina, Kostić, Jovana, Harwood J., Adrian, Stevanović, Milena, Drakulić, Danijela, "Generation of induced pluripotent stem cells derived from patients with 22q11.2 deletion syndrome as a tool for studying neurodevelopmental disorders" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):84-84,
https://hdl.handle.net/21.15107/rcub_imagine_2138 .

TY  - JOUR
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
AU  - Petrović, Isidora
AU  - Drakulić, Danijela
AU  - Milivojević, Milena
AU  - Mojsin, Marija
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/7/6392
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1890
AB  - Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.
T2  - International Journal of Molecular Sciences
T2  - International Journal of Molecular Sciences
T1  - Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma
IS  - 7
SP  - 6392
VL  - 24
DO  - 10.3390/ijms24076392
ER  - 

@article{
author = "Stevanović, Milena and Kovačević-Grujičić, Nataša and Petrović, Isidora and Drakulić, Danijela and Milivojević, Milena and Mojsin, Marija",
year = "2023",
abstract = "Glioblastoma (GBM) continues to be the most devastating primary brain malignancy. Despite significant advancements in understanding basic GBM biology and enormous efforts in developing new therapeutic approaches, the prognosis for most GBM patients remains poor with a median survival time of 15 months. Recently, the interplay between the SOX (SRY-related HMG-box) genes and lncRNAs (long non-coding RNAs) has become the focus of GBM research. Both classes of molecules have an aberrant expression in GBM and play essential roles in tumor initiation, progression, therapy resistance, and recurrence. In GBM, SOX and lncRNAs crosstalk through numerous functional axes, some of which are part of the complex transcriptional and epigenetic regulatory mechanisms. This review provides a systematic summary of current literature data on the complex interplay between SOX genes and lncRNAs and represents an effort to underscore the effects of SOX/lncRNA crosstalk on the malignant properties of GBM cells. Furthermore, we highlight the significance of this crosstalk in searching for new biomarkers and therapeutic approaches in GBM treatment.",
journal = "International Journal of Molecular Sciences, International Journal of Molecular Sciences",
title = "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma",
number = "7",
pages = "6392",
volume = "24",
doi = "10.3390/ijms24076392"
}

Stevanović, M., Kovačević-Grujičić, N., Petrović, I., Drakulić, D., Milivojević, M.,& Mojsin, M.. (2023). Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences, 24(7), 6392.
https://doi.org/10.3390/ijms24076392

Stevanović M, Kovačević-Grujičić N, Petrović I, Drakulić D, Milivojević M, Mojsin M. Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma. in International Journal of Molecular Sciences. 2023;24(7):6392.
doi:10.3390/ijms24076392 .

Stevanović, Milena, Kovačević-Grujičić, Nataša, Petrović, Isidora, Drakulić, Danijela, Milivojević, Milena, Mojsin, Marija, "Crosstalk between SOX Genes and Long Non-Coding RNAs in Glioblastoma" in International Journal of Molecular Sciences, 24, no. 7 (2023):6392,
https://doi.org/10.3390/ijms24076392 . .

TY  - CONF
AU  - Kostić, Jovana
AU  - Drakulić, Danijela
AU  - Čuturilo, Goran
AU  - Petter, Olena
AU  - Perić, Mina
AU  - Simeunović, Ivana
AU  - Harwood J., Adrian
AU  - Stevanović, Milena
AU  - Kovačević-Grujičić, Nataša
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2122
AB  - Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), schizophrenia, and intellectual disability, represent important public health challenge in modern societies
with a prevalence of about 10 to 15% of all births and the tendency of increasing worldwide. They are
caused by disruption of early brain development. Treatments of NDDs are focused on symptoms due to
a limited understanding of underlying pathophysiological mechanisms. Individuals with the 22q11.2
Duplication Syndrome (22q11.2dup), caused by heterozygous 22q11.2 microduplication, have an elevated risk of developing NDDs. Literature data revealed that ASD is detected in 14-25% of patients with
22q11.2dup while schizophrenia is less common in these patients than in the general population, suggesting that 22q11.2 duplication might be protective against schizophrenia.
Methods: Genomic and clinical findingsin patients with 22q11.2dup were analyzed and peripheral blood
mononuclear cells of patients with 22q11.2dup were reprogrammed.
Results: We formed a cohort of 8 patients with 22q11.2dup. The majority of patientsin our cohort have
microduplication of approximately 3Mb (80%). Also, the majority of them are familial cases and in 67%
of cases, the 22q11.2 microduplication is inherited from the mother. Congenital heart defects were detected in 25% of our patients, while all tested patients have facial dysmorphism. iPSCs were generated
from three patients with a familial form of 22q11.2dup and their mothers.
Conclusion: A cohort of patients with 22q11.2dup is formed and iPSCs were generated which can be
used as a model system for studying NDDs.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders
EP  - 66
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2122
ER  - 

@conference{
author = "Kostić, Jovana and Drakulić, Danijela and Čuturilo, Goran and Petter, Olena and Perić, Mina and Simeunović, Ivana and Harwood J., Adrian and Stevanović, Milena and Kovačević-Grujičić, Nataša",
year = "2023",
abstract = "Introduction: Neurodevelopmental disorders (NDDs), such as autism spectrum disorders (ASD), schizophrenia, and intellectual disability, represent important public health challenge in modern societies
with a prevalence of about 10 to 15% of all births and the tendency of increasing worldwide. They are
caused by disruption of early brain development. Treatments of NDDs are focused on symptoms due to
a limited understanding of underlying pathophysiological mechanisms. Individuals with the 22q11.2
Duplication Syndrome (22q11.2dup), caused by heterozygous 22q11.2 microduplication, have an elevated risk of developing NDDs. Literature data revealed that ASD is detected in 14-25% of patients with
22q11.2dup while schizophrenia is less common in these patients than in the general population, suggesting that 22q11.2 duplication might be protective against schizophrenia.
Methods: Genomic and clinical findingsin patients with 22q11.2dup were analyzed and peripheral blood
mononuclear cells of patients with 22q11.2dup were reprogrammed.
Results: We formed a cohort of 8 patients with 22q11.2dup. The majority of patientsin our cohort have
microduplication of approximately 3Mb (80%). Also, the majority of them are familial cases and in 67%
of cases, the 22q11.2 microduplication is inherited from the mother. Congenital heart defects were detected in 25% of our patients, while all tested patients have facial dysmorphism. iPSCs were generated
from three patients with a familial form of 22q11.2dup and their mothers.
Conclusion: A cohort of patients with 22q11.2dup is formed and iPSCs were generated which can be
used as a model system for studying NDDs.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2122"
}

Kostić, J., Drakulić, D., Čuturilo, G., Petter, O., Perić, M., Simeunović, I., Harwood J., A., Stevanović, M.,& Kovačević-Grujičić, N.. (2023). Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 66-66.
https://hdl.handle.net/21.15107/rcub_imagine_2122

Kostić J, Drakulić D, Čuturilo G, Petter O, Perić M, Simeunović I, Harwood J. A, Stevanović M, Kovačević-Grujičić N. Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:66-66.
https://hdl.handle.net/21.15107/rcub_imagine_2122 .

Kostić, Jovana, Drakulić, Danijela, Čuturilo, Goran, Petter, Olena, Perić, Mina, Simeunović, Ivana, Harwood J., Adrian, Stevanović, Milena, Kovačević-Grujičić, Nataša, "Establishment of induced pluripotent stem cells from patients with 22q11.2 duplication syndrome as a model system for studying neurodevelopmental disorders" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):66-66,
https://hdl.handle.net/21.15107/rcub_imagine_2122 .

TY  - CONF
AU  - Drakulić, Danijela
AU  - Rakonjac, Marijana
AU  - Cuturilo, Goran
AU  - Kovačević-Grujičić, Nataša
AU  - Kušić-Tišma, Jelena
AU  - Morić, Ivana
AU  - Zukić, Branka
AU  - Stevanović, Milena
PY  - 2023
UR  - https://belbi.bg.ac.rs/
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2036
AB  - 22q11.2 deletion syndrome (22q11.2DS is caused by 22q11.2 microdeletion, one of
the strongest known risk factors for development of neurodevelopmental disorders.
About 70% patients with 22q11.2DS have speech and language impairments. In the
literature, there is no data about articulatory characteristics of phonemes of children
with 22q11.2DS, monolingual native speakers of South Slavic languages. Here we, by
applying Global Articulation Test, analyzed articulatory characteristics of phonemes of
children with 22q11.2DS, monolingual native speakers of the Serbian language (group
E1), children with a phenotype resembling 22q11.2DS but without the microdeletion
(group E2), children with non-syndromic congenital heart malformations (since children
with these malformations may exhibit a speech and language impairments) (group
E3) and their peers with typical speech-sound development (group C). Results of PCA
indicated that the groups can be distinguished based on the pronunciation of phonemes,
and that the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩”
contributes the most to the variability between the groups. Results of AHP revealed that
the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩” was
rated the worst in the group E1. In conclusion, obtained results indicate that the presence
of 22q11.2 microdeletion influences articulation skills of carriers.
PB  - Belgrade : Institute of molecular genetics and genetic engineering
C3  - 4th Belgrade Bioinformatics Conference
T1  - Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome
EP  - 91
SP  - 91
VL  - 4
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2036
ER  - 

@conference{
author = "Drakulić, Danijela and Rakonjac, Marijana and Cuturilo, Goran and Kovačević-Grujičić, Nataša and Kušić-Tišma, Jelena and Morić, Ivana and Zukić, Branka and Stevanović, Milena",
year = "2023",
abstract = "22q11.2 deletion syndrome (22q11.2DS is caused by 22q11.2 microdeletion, one of
the strongest known risk factors for development of neurodevelopmental disorders.
About 70% patients with 22q11.2DS have speech and language impairments. In the
literature, there is no data about articulatory characteristics of phonemes of children
with 22q11.2DS, monolingual native speakers of South Slavic languages. Here we, by
applying Global Articulation Test, analyzed articulatory characteristics of phonemes of
children with 22q11.2DS, monolingual native speakers of the Serbian language (group
E1), children with a phenotype resembling 22q11.2DS but without the microdeletion
(group E2), children with non-syndromic congenital heart malformations (since children
with these malformations may exhibit a speech and language impairments) (group
E3) and their peers with typical speech-sound development (group C). Results of PCA
indicated that the groups can be distinguished based on the pronunciation of phonemes,
and that the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩”
contributes the most to the variability between the groups. Results of AHP revealed that
the pronunciation of the phonemes “Č ⟨tʃ⟩”, “Dž ⟨ʤ⟩”, “Š ⟨∫⟩”, “Ž ⟨ʒ⟩”, “R”, and “Lj ⟨ʎ⟩” was
rated the worst in the group E1. In conclusion, obtained results indicate that the presence
of 22q11.2 microdeletion influences articulation skills of carriers.",
publisher = "Belgrade : Institute of molecular genetics and genetic engineering",
journal = "4th Belgrade Bioinformatics Conference",
title = "Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome",
pages = "91-91",
volume = "4",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2036"
}

Drakulić, D., Rakonjac, M., Cuturilo, G., Kovačević-Grujičić, N., Kušić-Tišma, J., Morić, I., Zukić, B.,& Stevanović, M.. (2023). Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome. in 4th Belgrade Bioinformatics Conference
Belgrade : Institute of molecular genetics and genetic engineering., 4, 91-91.
https://hdl.handle.net/21.15107/rcub_imagine_2036

Drakulić D, Rakonjac M, Cuturilo G, Kovačević-Grujičić N, Kušić-Tišma J, Morić I, Zukić B, Stevanović M. Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome. in 4th Belgrade Bioinformatics Conference. 2023;4:91-91.
https://hdl.handle.net/21.15107/rcub_imagine_2036 .

Drakulić, Danijela, Rakonjac, Marijana, Cuturilo, Goran, Kovačević-Grujičić, Nataša, Kušić-Tišma, Jelena, Morić, Ivana, Zukić, Branka, Stevanović, Milena, "Application of principal component analysis (PCA) and analytical hierarchy process (AHP) in analysis of articulatory characteristics of phonemes of children with 22q11.2 Deletion Syndrome" in 4th Belgrade Bioinformatics Conference, 4 (2023):91-91,
https://hdl.handle.net/21.15107/rcub_imagine_2036 .

TY  - JOUR
AU  - Marković, Zoran
AU  - Budimir, Milica
AU  - Danko, Martin
AU  - Milivojević, Dušan
AU  - Kubat, Pavel
AU  - Zmejkoski, Danica
AU  - Pavlović, Vladimir
AU  - Mojsin, Marija
AU  - Stevanović, Milena
AU  - Todorović Marković, Biljana
PY  - 2023
UR  - https://www.beilstein-journals.org/bjnano/articles/14/17
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/1889
AB  - Carbon quantum dots as a novel type of carbon nanomaterials have attracted the attention of many researchers because of their
unique optical, antibacterial, and anticancer properties as well as their biocompatibility. In this study, for the first time, carbon
quantum dots were prepared from o-phenylenediamine dissolved in toluene by a solvothermal route. Subsequently, the prepared
carbon quantum dots were encapsulated into polyurethane films by a swelling–encapsulation–shrink method. Analyses of the
results obtained by different characterization methods (AFM, TEM, EDS, FTIR, photoluminescence, and EPR) indicate the significant influence of the precursor on structural, chemical, and optical properties. Antibacterial and cytotoxicity tests showed that these
dots did not have any antibacterial potential, because of the low extent of reactive oxygen species production, and showed low dark
cytotoxicity. By investigating the cellular uptake, it was established that these dots penetrated the HeLa cells and could be used as
probes for bioimaging.
T2  - Beilstein Journal of Nanotechnology
T2  - Beilstein Journal of NanotechnologyBeilstein J. Nanotechnol.
T1  - Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine
EP  - 174
IS  - 1
SP  - 165
VL  - 14
DO  - 10.3762/bjnano.14.17
ER  - 

@article{
author = "Marković, Zoran and Budimir, Milica and Danko, Martin and Milivojević, Dušan and Kubat, Pavel and Zmejkoski, Danica and Pavlović, Vladimir and Mojsin, Marija and Stevanović, Milena and Todorović Marković, Biljana",
year = "2023",
abstract = "Carbon quantum dots as a novel type of carbon nanomaterials have attracted the attention of many researchers because of their
unique optical, antibacterial, and anticancer properties as well as their biocompatibility. In this study, for the first time, carbon
quantum dots were prepared from o-phenylenediamine dissolved in toluene by a solvothermal route. Subsequently, the prepared
carbon quantum dots were encapsulated into polyurethane films by a swelling–encapsulation–shrink method. Analyses of the
results obtained by different characterization methods (AFM, TEM, EDS, FTIR, photoluminescence, and EPR) indicate the significant influence of the precursor on structural, chemical, and optical properties. Antibacterial and cytotoxicity tests showed that these
dots did not have any antibacterial potential, because of the low extent of reactive oxygen species production, and showed low dark
cytotoxicity. By investigating the cellular uptake, it was established that these dots penetrated the HeLa cells and could be used as
probes for bioimaging.",
journal = "Beilstein Journal of Nanotechnology, Beilstein Journal of NanotechnologyBeilstein J. Nanotechnol.",
title = "Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine",
pages = "174-165",
number = "1",
volume = "14",
doi = "10.3762/bjnano.14.17"
}

Marković, Z., Budimir, M., Danko, M., Milivojević, D., Kubat, P., Zmejkoski, D., Pavlović, V., Mojsin, M., Stevanović, M.,& Todorović Marković, B.. (2023). Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology, 14(1), 165-174.
https://doi.org/10.3762/bjnano.14.17

Marković Z, Budimir M, Danko M, Milivojević D, Kubat P, Zmejkoski D, Pavlović V, Mojsin M, Stevanović M, Todorović Marković B. Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine. in Beilstein Journal of Nanotechnology. 2023;14(1):165-174.
doi:10.3762/bjnano.14.17 .

Marković, Zoran, Budimir, Milica, Danko, Martin, Milivojević, Dušan, Kubat, Pavel, Zmejkoski, Danica, Pavlović, Vladimir, Mojsin, Marija, Stevanović, Milena, Todorović Marković, Biljana, "Structural, optical, and bioimaging characterization of carbon quantum dots solvothermally synthesized from o-phenylenediamine" in Beilstein Journal of Nanotechnology, 14, no. 1 (2023):165-174,
https://doi.org/10.3762/bjnano.14.17 . .

TY  - CONF
AU  - Miletić, Aleksandra
AU  - Cuturilo, Goran
AU  - Ruml Stojanović, Jelena
AU  - Drakulić, Danijela
AU  - Mijović, Marija
AU  - Bosankić, Brankica
AU  - Petrović, Hristina
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2180
AB  - Background/Objectives: Genetic tests may facilitate rapid and
effective diagnostics but unfortunately their high costs usually
limit their application in all patients (1). We aimed to investigate
the utility of rapid, cost effective and high sensitive Multiplex
ligation probe amplification analysis (MLPA) for detection copy
number variants (CNV) in newborns with critical CHD, admitted to
the Neonatal Intensive Care Unit (NICU).
Methods: Study included 100 consecutive newborns admitted
to the NICU, University Children’s Hospital in Belgrade from
August 2014 to September 2019. Patients with viable trisomies
(21, 18 and 13) were excluded. All participants were tested by
MLPA analysis using SALSA MLPA P250-B2 Di George and SALSA
MLPA P311-B1 Congenital Heart Disease probemixes (MRC Holland,
The Netherland).
Results: Pathogenic CNVs were identified in ten (10%) patients.
Nine of them had 22q11.2 deletion detected by both kits while
one patient had 3p25 deletion detected by P311 kit.
Conclusion: Genetic evaluation of all newborns with critical
CHD admitted to the NICU by rapid and inexpensive MLPA analysis
using combination P250 and P311 SALSA probemixes could
contribute to high detection rate of pathogenic variants.
PB  - Springer Nature
C3  - European Journal of Human Genetics
T1  - 22q11.2 microdeletion is the most common genomic abnormality in Serbian newborns with critical congenital heart disease and could be rapidly detected by Multiplex ligation probe amplification analysis
EP  - 140
IS  - Suppl 1
SP  - 140
VL  - 31
DO  - 10.1038/s41431-023-01339-3
ER  - 

@conference{
author = "Miletić, Aleksandra and Cuturilo, Goran and Ruml Stojanović, Jelena and Drakulić, Danijela and Mijović, Marija and Bosankić, Brankica and Petrović, Hristina and Stevanović, Milena",
year = "2023",
abstract = "Background/Objectives: Genetic tests may facilitate rapid and
effective diagnostics but unfortunately their high costs usually
limit their application in all patients (1). We aimed to investigate
the utility of rapid, cost effective and high sensitive Multiplex
ligation probe amplification analysis (MLPA) for detection copy
number variants (CNV) in newborns with critical CHD, admitted to
the Neonatal Intensive Care Unit (NICU).
Methods: Study included 100 consecutive newborns admitted
to the NICU, University Children’s Hospital in Belgrade from
August 2014 to September 2019. Patients with viable trisomies
(21, 18 and 13) were excluded. All participants were tested by
MLPA analysis using SALSA MLPA P250-B2 Di George and SALSA
MLPA P311-B1 Congenital Heart Disease probemixes (MRC Holland,
The Netherland).
Results: Pathogenic CNVs were identified in ten (10%) patients.
Nine of them had 22q11.2 deletion detected by both kits while
one patient had 3p25 deletion detected by P311 kit.
Conclusion: Genetic evaluation of all newborns with critical
CHD admitted to the NICU by rapid and inexpensive MLPA analysis
using combination P250 and P311 SALSA probemixes could
contribute to high detection rate of pathogenic variants.",
publisher = "Springer Nature",
journal = "European Journal of Human Genetics",
title = "22q11.2 microdeletion is the most common genomic abnormality in Serbian newborns with critical congenital heart disease and could be rapidly detected by Multiplex ligation probe amplification analysis",
pages = "140-140",
number = "Suppl 1",
volume = "31",
doi = "10.1038/s41431-023-01339-3"
}

Miletić, A., Cuturilo, G., Ruml Stojanović, J., Drakulić, D., Mijović, M., Bosankić, B., Petrović, H.,& Stevanović, M.. (2023). 22q11.2 microdeletion is the most common genomic abnormality in Serbian newborns with critical congenital heart disease and could be rapidly detected by Multiplex ligation probe amplification analysis. in European Journal of Human Genetics
Springer Nature., 31(Suppl 1), 140-140.
https://doi.org/10.1038/s41431-023-01339-3

Miletić A, Cuturilo G, Ruml Stojanović J, Drakulić D, Mijović M, Bosankić B, Petrović H, Stevanović M. 22q11.2 microdeletion is the most common genomic abnormality in Serbian newborns with critical congenital heart disease and could be rapidly detected by Multiplex ligation probe amplification analysis. in European Journal of Human Genetics. 2023;31(Suppl 1):140-140.
doi:10.1038/s41431-023-01339-3 .

Miletić, Aleksandra, Cuturilo, Goran, Ruml Stojanović, Jelena, Drakulić, Danijela, Mijović, Marija, Bosankić, Brankica, Petrović, Hristina, Stevanović, Milena, "22q11.2 microdeletion is the most common genomic abnormality in Serbian newborns with critical congenital heart disease and could be rapidly detected by Multiplex ligation probe amplification analysis" in European Journal of Human Genetics, 31, no. Suppl 1 (2023):140-140,
https://doi.org/10.1038/s41431-023-01339-3 . .

TY  - CONF
AU  - Lazić, Adrijana
AU  - Drakulić, Danijela
AU  - Kovačević-Grujičić, Nataša
AU  - Perić, Mina
AU  - Petrakis, Spyros
AU  - Linden, David
AU  - Harwood, Adrian
AU  - Stevanović, Milena
PY  - 2023
UR  - https://imagine.imgge.bg.ac.rs/handle/123456789/2110
AB  - Introduction: Neurodevelopmental disorders (NDDs) are a group of complex and heterogeneous disorders that include autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. However, underlying pathophysiological mechanisms are mostly unknown. In order to get better understanding of the underlying mechanisms and to discover potential therapeutics we have focused our research on 22q11.2 Deletion Syndrome (22q11.2DS), caused by microdeletion of the region q11.2 of chromosome 22 and associated with a high risk for NDDs. Methods: To study molecular mechanisms underlying intrafamilial phenotypic variability, we have identified families with the inherited form of 22q11.2DS with the aim of conducting the following analyses: whole genome sequencing in order to detect additional genetic variation(s) present in the affected child; generation of induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells; analysis of the effects of 22q11.2 microdeletion on neural differentiation including organoids as 3D model system; transcriptome analysis of iPSC-derived neurons and astrocytesto determine differentially expressed gene sets and dysregulated pathways; and testing the metabolic changes and drug responsiveness of neurons and astrocytes by high-throughput cell-based assays. Results: Peripheral blood mononuclear cells of the families with inherited form of 22q11.2DS were reprogrammed and established iPSCs were characterized. Generated iPSCs will be subjected to the further analyses. Conclusion: Currently, most of the treatments of NDDs are symptom-based due to limited understanding of underlying pathophysiological mechanisms. It is expected that patient-derived iPSCs will enable a deeper understanding of unique disease mechanisms and may also provide a significant contribution in preclinical drug development.
PB  - Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade
C3  - CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
T1  - 22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders
EP  - 31
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_imagine_2110
ER  - 

@conference{
author = "Lazić, Adrijana and Drakulić, Danijela and Kovačević-Grujičić, Nataša and Perić, Mina and Petrakis, Spyros and Linden, David and Harwood, Adrian and Stevanović, Milena",
year = "2023",
abstract = "Introduction: Neurodevelopmental disorders (NDDs) are a group of complex and heterogeneous disorders that include autism spectrum disorders, intellectual disability, schizophrenia and bipolar disorder. However, underlying pathophysiological mechanisms are mostly unknown. In order to get better understanding of the underlying mechanisms and to discover potential therapeutics we have focused our research on 22q11.2 Deletion Syndrome (22q11.2DS), caused by microdeletion of the region q11.2 of chromosome 22 and associated with a high risk for NDDs. Methods: To study molecular mechanisms underlying intrafamilial phenotypic variability, we have identified families with the inherited form of 22q11.2DS with the aim of conducting the following analyses: whole genome sequencing in order to detect additional genetic variation(s) present in the affected child; generation of induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells; analysis of the effects of 22q11.2 microdeletion on neural differentiation including organoids as 3D model system; transcriptome analysis of iPSC-derived neurons and astrocytesto determine differentially expressed gene sets and dysregulated pathways; and testing the metabolic changes and drug responsiveness of neurons and astrocytes by high-throughput cell-based assays. Results: Peripheral blood mononuclear cells of the families with inherited form of 22q11.2DS were reprogrammed and established iPSCs were characterized. Generated iPSCs will be subjected to the further analyses. Conclusion: Currently, most of the treatments of NDDs are symptom-based due to limited understanding of underlying pathophysiological mechanisms. It is expected that patient-derived iPSCs will enable a deeper understanding of unique disease mechanisms and may also provide a significant contribution in preclinical drug development.",
publisher = "Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade",
journal = "CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia",
title = "22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders",
pages = "31-31",
url = "https://hdl.handle.net/21.15107/rcub_imagine_2110"
}

Lazić, A., Drakulić, D., Kovačević-Grujičić, N., Perić, M., Petrakis, S., Linden, D., Harwood, A.,& Stevanović, M.. (2023). 22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia
Institute of Molecular Genetics and Genetic Engineering (IMGGE), University of Belgrade., 31-31.
https://hdl.handle.net/21.15107/rcub_imagine_2110

Lazić A, Drakulić D, Kovačević-Grujičić N, Perić M, Petrakis S, Linden D, Harwood A, Stevanović M. 22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders. in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia. 2023;:31-31.
https://hdl.handle.net/21.15107/rcub_imagine_2110 .

Lazić, Adrijana, Drakulić, Danijela, Kovačević-Grujičić, Nataša, Perić, Mina, Petrakis, Spyros, Linden, David, Harwood, Adrian, Stevanović, Milena, "22q11.2 Deletion syndrome as a tool for modelling and research of neurodevelopmental disorders" in CoMBoS2 – the Second Congress of Molecular Biologists of Serbia, Abstract Book – Trends in Molecular Biology, Special issue 06-08 October 2023, Belgrade, Serbia (2023):31-31,
https://hdl.handle.net/21.15107/rcub_imagine_2110 .